Cp4.1LG16g01080 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG16g01080
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionGamma Purothionin
LocationCp4.1LG16 : 2109294 .. 2109600 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAACAAATCCTTCCTCTCCATGCTCCTATTGGTCCTCCTCATCTTCTCTTCTTCAGGTAATCAAAGTCTAACCCTACCATCTTTGCCATTTCTTCTTTCTTCCTTTCTTCCTAATATGTGTGTTATTTGCAGAAGAGATGATGGGTCGTGGTGCTGAGGCTCGAATATGCAAGTCCAGGAACCACCACTACCATGGCCTCTGCTTTAGCCACAAGTGCGCTACTCGTTGCCGAAAGGAAGGCTACCTTGGCGGTCATTGTCACCACTTCCCCCGCCATTGCTATTGCACTCACAGTTGTTAA

mRNA sequence

ATGATGAACAAATCCTTCCTCTCCATGCTCCTATTGGTCCTCCTCATCTTCTCTTCTTCAGAAGAGATGATGGGTCGTGGTGCTGAGGCTCGAATATGCAAGTCCAGGAACCACCACTACCATGGCCTCTGCTTTAGCCACAAGTGCGCTACTCGTTGCCGAAAGGAAGGCTACCTTGGCGGTCATTGTCACCACTTCCCCCGCCATTGCTATTGCACTCACAGTTGTTAA

Coding sequence (CDS)

ATGATGAACAAATCCTTCCTCTCCATGCTCCTATTGGTCCTCCTCATCTTCTCTTCTTCAGAAGAGATGATGGGTCGTGGTGCTGAGGCTCGAATATGCAAGTCCAGGAACCACCACTACCATGGCCTCTGCTTTAGCCACAAGTGCGCTACTCGTTGCCGAAAGGAAGGCTACCTTGGCGGTCATTGTCACCACTTCCCCCGCCATTGCTATTGCACTCACAGTTGTTAA

Protein sequence

MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLGGHCHHFPRHCYCTHSC
Homology
[Close]
BLAST of Cp4.1LG16g01080 vs. Swiss-Prot
Match: DEF06_ARATH (Defensin-like protein 6 OS=Arabidopsis thaliana GN=PDF2.5 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.2e-11
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 1 

Query: 2  MNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLG 61
          M   F +   L+L++FSS E +   G E R C+S++HH+  +C S H CA  CR EG+ G
Sbjct: 1  MENKFFAAFFLLLVLFSSQEII---GGEGRTCQSKSHHFKYMCTSNHNCAIVCRNEGFSG 60

Query: 62 GHCHHFPRHCYCTHSC 77
          G CH F R CYCT  C
Sbjct: 61 GRCHGFHRRCYCTRLC 73

[Close]
BLAST of Cp4.1LG16g01080 vs. Swiss-Prot
Match: DEF2_CAPAN (Defensin J1-2 OS=Capsicum annuum PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.5e-09
Identity = 31/75 (41.33%), Postives = 47/75 (62.67%), Query Frame = 1 

Query: 3  NKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLGG 62
          +K   ++ L+++L+F++     G  AEAR C+S++H + GLCFS   C + C  EG+ GG
Sbjct: 5  SKVIATIFLMMMLVFAT-----GMVAEARTCESQSHRFKGLCFSKSNCGSVCHTEGFNGG 64

Query: 63 HCHHFPRHCYCTHSC 77
          HC  F R C+CT  C
Sbjct: 65 HCRGFRRRCFCTRHC 74

[Close]
BLAST of Cp4.1LG16g01080 vs. Swiss-Prot
Match: DEF01_ARATH (Defensin-like protein 1 OS=Arabidopsis thaliana GN=PDF2.3 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 6.1e-08
Identity = 32/75 (42.67%), Postives = 43/75 (57.33%), Query Frame = 1 

Query: 6  FLSMLLLVLLIFSSSEEMMGRG---AEARICKSRNHHYHGLCFS-HKCATRCRKEGYLGG 65
          F+S  LL+ ++F ++    G G    EAR C+S++H + G C S H CA  C  EG+ GG
Sbjct: 7  FISAALLLFMVFIAT----GMGPVTVEARTCESKSHRFKGPCVSTHNCANVCHNEGFGGG 66

Query: 66 HCHHFPRHCYCTHSC 77
           C  F R CYCT  C
Sbjct: 67 KCRGFRRRCYCTRHC 77

[Close]
BLAST of Cp4.1LG16g01080 vs. Swiss-Prot
Match: DEF_NELNU (Defensin-like protein OS=Nelumbo nucifera PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.5e-06
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 1 

Query: 6  FLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSH-KCATRCRKEGYLGGHCH 65
          F S++L++LL+  ++ EM  + AEAR C+S++H + G C S   CA+ C+ EG+  G C 
Sbjct: 8  FSSLVLVLLLV--TATEMGPKVAEARTCESQSHRFKGACLSDTNCASVCQTEGFPAGDCK 67

Query: 66 HFPRHCYCTHSC 77
             R C+C   C
Sbjct: 68 GARRRCFCVKPC 77

[Close]
BLAST of Cp4.1LG16g01080 vs. TrEMBL
Match: A0A0A0LN65_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G340400 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.0e-17
Identity = 47/77 (61.04%), Postives = 58/77 (75.32%), Query Frame = 1 

Query: 1   MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCF-SHKCATRCRKEGYL 60
           MMNKS +  LL++LLIFS+SEEMMG GAEAR+C+S++HH+HG C   H CA  CR EG+ 
Sbjct: 31  MMNKSLVPTLLVLLLIFSASEEMMGGGAEARMCQSQSHHFHGPCVRDHNCALVCRTEGFS 90

Query: 61  GGHCHHFPRHCYCTHSC 77
           GG C  F R C+CTH C
Sbjct: 91  GGECIGFRRRCFCTHRC 107

[Close]
BLAST of Cp4.1LG16g01080 vs. TrEMBL
Match: W5RNL9_CITLA (Defensin-like protein 2 OS=Citrullus lanatus GN=PDF2.2 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 5.6e-16
Identity = 48/78 (61.54%), Postives = 56/78 (71.79%), Query Frame = 1 

Query: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRG-AEARICKSRNHHYHGLCF-SHKCATRCRKEGY 60
          MMNKSFL  LLL+LLIFS+SEEMMG G AE R+CKS++H +HG C   H CA  CR EG+
Sbjct: 1  MMNKSFLPTLLLLLLIFSASEEMMGGGRAEGRVCKSQSHSFHGPCMRDHNCAVVCRTEGF 60

Query: 61 LGGHCHHFPRHCYCTHSC 77
           GG C  F R C+CT  C
Sbjct: 61 SGGDCVGFRRRCFCTRLC 78

[Close]
BLAST of Cp4.1LG16g01080 vs. TrEMBL
Match: Q9FQ14_CITPA (Proteinase inhibitor se60-like protein OS=Citrus paradisi PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 9.2e-11
Identity = 36/74 (48.65%), Postives = 50/74 (67.57%), Query Frame = 1 

Query: 4  KSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLGGH 63
          KSF  + LL+L++F+S E M+   AE R+C+S++HH+HG CFS H CA  CR EG+ GG 
Sbjct: 2  KSFFGIFLLLLILFASQEIMVP--AEGRVCQSQSHHFHGACFSHHNCAFVCRNEGFSGGK 61

Query: 64 CHHFPRHCYCTHSC 77
          C    R C+C+  C
Sbjct: 62 CRGVRRRCFCSKLC 73

[Close]
BLAST of Cp4.1LG16g01080 vs. TrEMBL
Match: V4SLE8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026887mg PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 9.2e-11
Identity = 36/74 (48.65%), Postives = 50/74 (67.57%), Query Frame = 1 

Query: 4  KSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLGGH 63
          KSF  + LL+L++F+S E M+   AE R+C+S++HH+HG CFS H CA  CR EG+ GG 
Sbjct: 2  KSFFGIFLLLLILFASQEMMVP--AEGRVCQSQSHHFHGACFSHHNCAFVCRNEGFSGGK 61

Query: 64 CHHFPRHCYCTHSC 77
          C    R C+C+  C
Sbjct: 62 CRGARRRCFCSKLC 73

[Close]
BLAST of Cp4.1LG16g01080 vs. TrEMBL
Match: V4UJZ8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026887mg PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.7e-09
Identity = 35/74 (47.30%), Postives = 48/74 (64.86%), Query Frame = 1 

Query: 4  KSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLGGH 63
          KSF  + LL+L++F+S    M   AE R+C+S++HH+HG CFS H CA  CR EG+ GG 
Sbjct: 2  KSFFGIFLLLLILFASQ---MMVPAEGRVCQSQSHHFHGACFSHHNCAFVCRNEGFSGGK 61

Query: 64 CHHFPRHCYCTHSC 77
          C    R C+C+  C
Sbjct: 62 CRGARRRCFCSKLC 72

[Close]
BLAST of Cp4.1LG16g01080 vs. TAIR10
Match: AT5G63660.1 (AT5G63660.1 Scorpion toxin-like knottin superfamily protein)

HSP 1 Score: 70.1 bits (170), Expect = 6.7e-13
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 1 

Query: 2  MNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLG 61
          M   F +   L+L++FSS E +   G E R C+S++HH+  +C S H CA  CR EG+ G
Sbjct: 1  MENKFFAAFFLLLVLFSSQEII---GGEGRTCQSKSHHFKYMCTSNHNCAIVCRNEGFSG 60

Query: 62 GHCHHFPRHCYCTHSC 77
          G CH F R CYCT  C
Sbjct: 61 GRCHGFHRRCYCTRLC 73

[Close]
BLAST of Cp4.1LG16g01080 vs. TAIR10
Match: AT2G02130.1 (AT2G02130.1 low-molecular-weight cysteine-rich 68)

HSP 1 Score: 57.8 bits (138), Expect = 3.4e-09
Identity = 32/75 (42.67%), Postives = 43/75 (57.33%), Query Frame = 1 

Query: 6  FLSMLLLVLLIFSSSEEMMGRG---AEARICKSRNHHYHGLCFS-HKCATRCRKEGYLGG 65
          F+S  LL+ ++F ++    G G    EAR C+S++H + G C S H CA  C  EG+ GG
Sbjct: 7  FISAALLLFMVFIAT----GMGPVTVEARTCESKSHRFKGPCVSTHNCANVCHNEGFGGG 66

Query: 66 HCHHFPRHCYCTHSC 77
           C  F R CYCT  C
Sbjct: 67 KCRGFRRRCYCTRHC 77

[Close]
BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: gi|700207128|gb|KGN62247.1| (hypothetical protein Csa_2G340400 [Cucumis sativus])

HSP 1 Score: 97.1 bits (240), Expect = 1.5e-17
Identity = 47/77 (61.04%), Postives = 58/77 (75.32%), Query Frame = 1 

Query: 1   MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCF-SHKCATRCRKEGYL 60
           MMNKS +  LL++LLIFS+SEEMMG GAEAR+C+S++HH+HG C   H CA  CR EG+ 
Sbjct: 31  MMNKSLVPTLLVLLLIFSASEEMMGGGAEARMCQSQSHHFHGPCVRDHNCALVCRTEGFS 90

Query: 61  GGHCHHFPRHCYCTHSC 77
           GG C  F R C+CTH C
Sbjct: 91  GGECIGFRRRCFCTHRC 107

[Close]
BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: gi|558067066|gb|AHA50468.1| (defensin-like protein 2 [Citrullus lanatus])

HSP 1 Score: 91.3 bits (225), Expect = 8.0e-16
Identity = 48/78 (61.54%), Postives = 56/78 (71.79%), Query Frame = 1 

Query: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRG-AEARICKSRNHHYHGLCF-SHKCATRCRKEGY 60
          MMNKSFL  LLL+LLIFS+SEEMMG G AE R+CKS++H +HG C   H CA  CR EG+
Sbjct: 1  MMNKSFLPTLLLLLLIFSASEEMMGGGRAEGRVCKSQSHSFHGPCMRDHNCAVVCRTEGF 60

Query: 61 LGGHCHHFPRHCYCTHSC 77
           GG C  F R C+CT  C
Sbjct: 61 SGGDCVGFRRRCFCTRLC 78

[Close]
BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: gi|11596184|gb|AAG38520.1|AF283535_1 (proteinase inhibitor se60-like protein [Citrus x paradisi])

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-10
Identity = 36/74 (48.65%), Postives = 50/74 (67.57%), Query Frame = 1 

Query: 4  KSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLGGH 63
          KSF  + LL+L++F+S E M+   AE R+C+S++HH+HG CFS H CA  CR EG+ GG 
Sbjct: 2  KSFFGIFLLLLILFASQEIMVP--AEGRVCQSQSHHFHGACFSHHNCAFVCRNEGFSGGK 61

Query: 64 CHHFPRHCYCTHSC 77
          C    R C+C+  C
Sbjct: 62 CRGVRRRCFCSKLC 73

[Close]
BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: gi|567867595|ref|XP_006426420.1| (hypothetical protein CICLE_v10026887mg [Citrus clementina])

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-10
Identity = 36/74 (48.65%), Postives = 50/74 (67.57%), Query Frame = 1 

Query: 4  KSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLGGH 63
          KSF  + LL+L++F+S E M+   AE R+C+S++HH+HG CFS H CA  CR EG+ GG 
Sbjct: 2  KSFFGIFLLLLILFASQEMMVP--AEGRVCQSQSHHFHGACFSHHNCAFVCRNEGFSGGK 61

Query: 64 CHHFPRHCYCTHSC 77
          C    R C+C+  C
Sbjct: 62 CRGARRRCFCSKLC 73

[Close]
BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: gi|15242860|ref|NP_201171.1| (defensin-like protein 6 [Arabidopsis thaliana])

HSP 1 Score: 70.1 bits (170), Expect = 1.9e-09
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 1 

Query: 2  MNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLG 61
          M   F +   L+L++FSS E +   G E R C+S++HH+  +C S H CA  CR EG+ G
Sbjct: 1  MENKFFAAFFLLLVLFSSQEII---GGEGRTCQSKSHHFKYMCTSNHNCAIVCRNEGFSG 60

Query: 62 GHCHHFPRHCYCTHSC 77
          G CH F R CYCT  C
Sbjct: 61 GRCHGFHRRCYCTRLC 73

The following BLAST results are available for this feature:
BLAST of Cp4.1LG16g01080 vs. Swiss-Prot
Match NameE-valueIdentityDescription
DEF06_ARATH1.2e-1146.05Defensin-like protein 6 OS=Arabidopsis thaliana GN=PDF2.5 PE=3 SV=1[more]
DEF2_CAPAN1.5e-0941.33Defensin J1-2 OS=Capsicum annuum PE=1 SV=1[more]
DEF01_ARATH6.1e-0842.67Defensin-like protein 1 OS=Arabidopsis thaliana GN=PDF2.3 PE=2 SV=1[more]
DEF_NELNU7.5e-0638.89Defensin-like protein OS=Nelumbo nucifera PE=3 SV=1[more]
BLAST of Cp4.1LG16g01080 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LN65_CUCSA1.0e-1761.04Uncharacterized protein OS=Cucumis sativus GN=Csa_2G340400 PE=3 SV=1[more]
W5RNL9_CITLA5.6e-1661.54Defensin-like protein 2 OS=Citrullus lanatus GN=PDF2.2 PE=2 SV=1[more]
Q9FQ14_CITPA9.2e-1148.65Proteinase inhibitor se60-like protein OS=Citrus paradisi PE=2 SV=1[more]
V4SLE8_9ROSI9.2e-1148.65Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026887mg PE=3 SV=1[more]
V4UJZ8_9ROSI1.7e-0947.30Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026887mg PE=3 SV=1[more]
BLAST of Cp4.1LG16g01080 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G63660.16.7e-1346.05 Scorpion toxin-like knottin superfamily protein[more]
AT2G02130.13.4e-0942.67 low-molecular-weight cysteine-rich 68[more]
BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700207128|gb|KGN62247.1|1.5e-1761.04hypothetical protein Csa_2G340400 [Cucumis sativus][more]
gi|558067066|gb|AHA50468.1|8.0e-1661.54defensin-like protein 2 [Citrullus lanatus][more]
gi|11596184|gb|AAG38520.1|AF283535_11.3e-1048.65proteinase inhibitor se60-like protein [Citrus x paradisi][more]
gi|567867595|ref|XP_006426420.1|1.3e-1048.65hypothetical protein CICLE_v10026887mg [Citrus clementina][more]
gi|15242860|ref|NP_201171.1|1.9e-0946.05defensin-like protein 6 [Arabidopsis thaliana][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006952defense response
Vocabulary: INTERPRO
TermDefinition
IPR008176Defensin_plant
IPR003614Scorpion_toxin-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g01080.1Cp4.1LG16g01080.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003614Knottin, scorpion toxin-likeGENE3DG3DSA:3.30.30.10coord: 31..76
score: 8.0
IPR003614Knottin, scorpion toxin-likeSMARTSM00505gth_4coord: 32..76
score: 4.
IPR003614Knottin, scorpion toxin-likeunknownSSF57095Scorpion toxin-likecoord: 31..76
score: 6.88
IPR008176Defensin, plantPRINTSPR00288PUROTHIONINcoord: 32..47
score: 9.0E-5coord: 61..75
score: 9.
NoneNo IPR availablePANTHERPTHR33147FAMILY NOT NAMEDcoord: 2..76
score: 5.5
NoneNo IPR availablePFAMPF00304Gamma-thionincoord: 31..76
score: 3.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG16g01080CmoCh20G010390Cucurbita moschata (Rifu)cmocpeB517
Cp4.1LG16g01080Cla007847Watermelon (97103) v1cpewmB288
Cp4.1LG16g01080MELO3C023203Melon (DHL92) v3.5.1cpemeB261
Cp4.1LG16g01080ClCG02G000130Watermelon (Charleston Gray)cpewcgB268
Cp4.1LG16g01080Lsi10G007770Bottle gourd (USVL1VR-Ls)cpelsiB222
Cp4.1LG16g01080MELO3C023203.2Melon (DHL92) v3.6.1cpemedB305
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG16g01080Wax gourdcpewgoB0359
Cp4.1LG16g01080Cucumber (Gy14) v1cgycpeB0606
Cp4.1LG16g01080Cucurbita maxima (Rimu)cmacpeB565
Cp4.1LG16g01080Wild cucumber (PI 183967)cpecpiB273
Cp4.1LG16g01080Cucumber (Chinese Long) v2cpecuB271
Cp4.1LG16g01080Bottle gourd (USVL1VR-Ls)cpelsiB225
Cp4.1LG16g01080Watermelon (Charleston Gray)cpewcgB274
Cp4.1LG16g01080Watermelon (97103) v1cpewmB302
Cp4.1LG16g01080Melon (DHL92) v3.5.1cpemeB268
Cp4.1LG16g01080Cucumber (Gy14) v2cgybcpeB194
Cp4.1LG16g01080Cucumber (Gy14) v2cgybcpeB199
Cp4.1LG16g01080Melon (DHL92) v3.6.1cpemedB312
Cp4.1LG16g01080Cucumber (Chinese Long) v3cpecucB0335
Cp4.1LG16g01080Cucumber (Chinese Long) v3cpecucB0338