Cp4.1LG15g08580 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG15g08580
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCytochrome P450 family protein
LocationCp4.1LG15 : 8444664 .. 8448088 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGCGGGAAAATTGTTATAAGCGAGTACGTAGGTTCGCCACTGTACAGGTATGGAGTTTTCCTTTCCGAGAATGATTGAAGTTCCAGGAGTTCAGTCTCTCCCGGTACGTTTTGTTGTTCATTTTTTGAAATTGAGGAGTTGTTTGTTAAGTAATGATCTGTGTTCTTCGTTTAAGTGTTGGTCTTAGAACGATTTAAGTTTCGGGGTTAAGCTTGGATGGGTTCCCCGGGGGAGAGAAATAAGATTCGCAATGGAAACAAGATTTAGTGTCTGGGGCATGTGCGAGGAAATGGGGAATTGGGATGAGGAACAAAGGGAGGTGCCGATGTGGTTGGTGGGGTTCCGTAACAGGGACACGTCTGTCATTGCCTCGTTAGGGTATAATACTCATTTTCCCCCTCATTAGCGTAAACTAGATACGTGGTCATGCAAATGCAACAGATATTTTCGTAGGGAACTTGTGAACTTGTGAAAAGTAAAACGACTTTGCTTCAATTATTGTCTAATTTGATGGTTACCATATAAAGCATAAGATCTTACCCAATTACATTTTCTTTATCAGCTTTTAATTGCTTTCGTTTTCCATCAACATGCTACAGACCAAACAAATATGTGCACAAACTTATATTATTTAAGTTCAACAGCAAATAGAGATTGGAAAATAAGTATTAGAATCGTATCCCAGTCGTCATGTGTTCCTTATTCTAAGGATACATATTGTATTAATGTATAGGATATACACTCTGTGTGCACATGCAAAGCCCACGATTCTAGCCTTACGATAACTTAATTTGTTATGAAAGTCATGCTTCTTCCTCAACTTCAACCCCAAGATTTTGACCATCCATTCATTTAAATATAAATCAAACAGCAATGCTCATTCGTTGACTTTCAACTTGTTCCGTTAAATATGTGGCTTCTCTTCCCAACAAACATAAATCTGTGTATATGGTTCAAGAATTTAGCTGCTTTTTGTTGGCAACAATGTTCTTGTTTTTATCGGCAATCTTAATAATATCCATTATTTTTGTGTTTAGAAGCATAAAGGGAAGCAAAAAGGTACCGCCTGGGCAAATGGGTCTGCCCTGGATTGGCGAAACCATGGCTTTCTACAGAGCCCAACGTAAAAACAGGTTGTTCGAAGAGTTTGTTGGGCCTCGAGTGGCTAAATATGGCAAAATCTTCAAAACAAGCTTGATGGGGTCGCCGACGGTGGTCGTAAATGGAGCTGAAGCCAACAGATTTTTCTTGTCCAATGAGTTTAAGTTGGTGGTAAGCTCATGGCCGTCGGCCTCTGTTCAGCTGATGGGCGAAGAATGCATCATGCAGAAGCAAGGTGAAAGCCACCGATGTGTGCGTGGCCTTATTGCCGCAAGCCTTGCCAGTTCGAGTCTCGAAGCTATGATGCCTAAATTGTGCACGACAATTCAGCTTTATTTGGACACCAAATGGCTTGGCCATGATATAATTAGTCTCTACCACTCGGCAAAAGTATTGACGTTTACCATAGTGTTTGAGTGTTTGATGGGTATCAAAGTGGAGCCACGTATGTTGACGACATTCGAGAGGGTTTTGGAAGGAGTTTTTGCTCCACCGTTCAGGTAGCTATTCAACTATACCCCTAATTCAAAAGTTTGGGCTGATTGATAGGTTATGATAAATTTAAATCTGGTTTGATAACGATTTCATTTTTAAAATTTCTTTCCTATCATATTTACTTTCTTACCTTTTTAATTTCAAACTTGACTCGATTTTTGAAAGTATTAGTAGAATGTGAATAGAGAAATTTATATATGAAATTTGTGTTTATAAACTTGATTTTCAAATACCAAAGGATATTAAATGGAACCCGAAGTTTTAATTATTTGTTCATATTCTTAACCTAGCTTTCCAGGCACACGGTTTTCGAGAGCGAAGAAAGCAAGGATGGAGATAGGGAAAATGCTAAGGGAGGTGGTGAGAGAGAAGAGGAGGAGAATTGAAGAAGAGCCCGAAGAAGAAGAATGTGAGTTGTTGCTTTCAAGGATGGTGAGAGCAATGATAAGAGGGGAGATCAGTGAAGTGGAAGTGGTGGACAATGTGGTTTTGCTTGTTTTTGCAGCTCATGATACAACCTCCTTCGCCATTGCCATGACTTTCAAAATGTTGGCTCAGCAGCCAAACTGCTACTCACTTCTTCTCCAGGGTAATTTTTTTTTTTCTTTAATCTTGTAAATTATATGTCAGTATCATCATCAATCAAAGAGCTGGGCGCCATTTCAACTTCCTTTCTTCAAAATACCATTTTGCTGATCTGAGTGAGAAATAAGATGTGTAAATTTGAGAAGAGAAGCCTCTTTACTTCTTGACAGAACATGCTGACATAGTGAGAAGCAAGGGAGCAGGCCAGAATATGACGATGGAAGATATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGTGAGAGCATTCGACTGTTTCCTCCTATATTTGGTTCATTCAGGAAAGCCATTACAGACATTGAATATGAGGGATTTTTCATTCCCAAGGGGTGGAAGGTGAAAATTTCCATAAATTCTTCTGCTTTTTCTTTACTACTGATACCCTGCAGCGTCAAATTGTAGTCCAGAAATGACCATCGTTTTACCTTGAATCATTTTGGTTGCGTGCAGGTCTTGTGGACGACGTATGGAACGCATCTCGAAGACGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCCCAGTATGTCTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAGCTGGCGAAATTGAACATCCTCATCTTTATACACTTGGTTGTAACTCGTTATGATTGGAGTTTAGTCCACCCAGATGAGACAATCATAATGGATCCCCTCCCTATCCCTTCTCATGGAATGCCAATTAAGATTTCTCCAAAATCAGAAGCTCGCCCCAATGTTCAGCAAATGTAAGCTCAGAAGAGAACAAAGACGATGCACTTAAGAGGATGTAAAACCTCTAGATATCAGACATAGTATACATAATTATGATTCTTTCCAACAGCAGACATAAAGATCTCATAAAAGGAAACCCTTTCTACATTTCACTATTGTTGCCGATCCAAGAATACAGTCTACTATATTTTAACTCAGGTGTATAACCAATAGCTAATGTAATATACGTCCAGTGGACAAAAAGAGTGATTCTTCAGTTTTTCATGATATCTACCGGGCAAAATTTTCAAAAACACTAAAGAGGTTACGGATCTTTAATGCACTGTTTAGTGTCTGGCAATAGGCGATGACTAATAGGTCTTACTCCATGCTTAAGCCAACGTTTCACCAGGAATATTTCTAATTTCCTTACCTGCAACCACAAAAAACCACAGAATAGAGTGAACAATGGTTCAGCATCTCCAATGG

mRNA sequence

TGGCGGGAAAATTGTTATAAGCGAGTACGTAGGTTCGCCACTGTACAGGTATGGAGTTTTCCTTTCCGAGAATGATTGAAGTTCCAGGAGTTCAGTCTCTCCCGAAGCATAAAGGGAAGCAAAAAGGTACCGCCTGGGCAAATGGGTCTGCCCTGGATTGGCGAAACCATGGCTTTCTACAGAGCCCAACGTAAAAACAGGTTGTTCGAAGAGTTTGTTGGGCCTCGAGTGGCTAAATATGGCAAAATCTTCAAAACAAGCTTGATGGGGTCGCCGACGGTGGTCGTAAATGGAGCTGAAGCCAACAGATTTTTCTTGTCCAATGAGTTTAAGTTGGTGGTAAGCTCATGGCCGTCGGCCTCTGTTCAGCTGATGGGCGAAGAATGCATCATGCAGAAGCAAGGTGAAAGCCACCGATGTGTGCGTGGCCTTATTGCCGCAAGCCTTGCCAGTTCGAGTCTCGAAGCTATGATGCCTAAATTGTGCACGACAATTCAGCTTTATTTGGACACCAAATGGCTTGGCCATGATATAATTAGTCTCTACCACTCGGCAAAAGTATTGACGTTTACCATAGTGTTTGAGTGTTTGATGGGTATCAAAGTGGAGCCACGTATGTTGACGACATTCGAGAGGGTTTTGGAAGGAGTTTTTGCTCCACCGTTCAGCTTTCCAGGCACACGGTTTTCGAGAGCGAAGAAAGCAAGGATGGAGATAGGGAAAATGCTAAGGGAGGTGGTGAGAGAGAAGAGGAGGAGAATTGAAGAAGAGCCCGAAGAAGAAGAATGTGAGTTGTTGCTTTCAAGGATGGTGAGAGCAATGATAAGAGGGGAGATCAGTGAAGTGGAAGTGGTGGACAATGTGGTTTTGCTTGTTTTTGCAGCTCATGATACAACCTCCTTCGCCATTGCCATGACTTTCAAAATGTTGGCTCAGCAGCCAAACTGCTACTCACTTCTTCTCCAGGAACATGCTGACATAGTGAGAAGCAAGGGAGCAGGCCAGAATATGACGATGGAAGATATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGTGAGAGCATTCGACTGTTTCCTCCTATATTTGGTTCATTCAGGAAAGCCATTACAGACATTGAATATGAGGGATTTTTCATTCCCAAGGGGTGGAAGGTCTTGTGGACGACGTATGGAACGCATCTCGAAGACGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCCCAGTATGTCTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAGCTGGCGAAATTGAACATCCTCATCTTTATACACTTGGTTGTAACTCGTTATGATTGGAGTTTAGTCCACCCAGATGAGACAATCATAATGGATCCCCTCCCTATCCCTTCTCATGGAATGCCAATTAAGATTTCTCCAAAATCAGAAGCTCGCCCCAATGTTCAGCAAATGTAAGCTCAGAAGAGAACAAAGACGATGCACTTAAGAGGATGTAAAACCTCTAGATATCAGACATAGTATACATAATTATGATTCTTTCCAACAGCAGACATAAAGATCTCATAAAAGGAAACCCTTTCTACATTTCACTATTGTTGCCGATCCAAGAATACAGTCTACTATATTTTAACTCAGGTGTATAACCAATAGCTAATGTAATATACGTCCAGTGGACAAAAAGAGTGATTCTTCAGTTTTTCATGATATCTACCGGGCAAAATTTTCAAAAACACTAAAGAGGTTACGGATCTTTAATGCACTGTTTAGTGTCTGGCAATAGGCGATGACTAATAGGTCTTACTCCATGCTTAAGCCAACGTTTCACCAGGAATATTTCTAATTTCCTTACCTGCAACCACAAAAAACCACAGAATAGAGTGAACAATGGTTCAGCATCTCCAATGG

Coding sequence (CDS)

ATGGGTCTGCCCTGGATTGGCGAAACCATGGCTTTCTACAGAGCCCAACGTAAAAACAGGTTGTTCGAAGAGTTTGTTGGGCCTCGAGTGGCTAAATATGGCAAAATCTTCAAAACAAGCTTGATGGGGTCGCCGACGGTGGTCGTAAATGGAGCTGAAGCCAACAGATTTTTCTTGTCCAATGAGTTTAAGTTGGTGGTAAGCTCATGGCCGTCGGCCTCTGTTCAGCTGATGGGCGAAGAATGCATCATGCAGAAGCAAGGTGAAAGCCACCGATGTGTGCGTGGCCTTATTGCCGCAAGCCTTGCCAGTTCGAGTCTCGAAGCTATGATGCCTAAATTGTGCACGACAATTCAGCTTTATTTGGACACCAAATGGCTTGGCCATGATATAATTAGTCTCTACCACTCGGCAAAAGTATTGACGTTTACCATAGTGTTTGAGTGTTTGATGGGTATCAAAGTGGAGCCACGTATGTTGACGACATTCGAGAGGGTTTTGGAAGGAGTTTTTGCTCCACCGTTCAGCTTTCCAGGCACACGGTTTTCGAGAGCGAAGAAAGCAAGGATGGAGATAGGGAAAATGCTAAGGGAGGTGGTGAGAGAGAAGAGGAGGAGAATTGAAGAAGAGCCCGAAGAAGAAGAATGTGAGTTGTTGCTTTCAAGGATGGTGAGAGCAATGATAAGAGGGGAGATCAGTGAAGTGGAAGTGGTGGACAATGTGGTTTTGCTTGTTTTTGCAGCTCATGATACAACCTCCTTCGCCATTGCCATGACTTTCAAAATGTTGGCTCAGCAGCCAAACTGCTACTCACTTCTTCTCCAGGAACATGCTGACATAGTGAGAAGCAAGGGAGCAGGCCAGAATATGACGATGGAAGATATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGTGAGAGCATTCGACTGTTTCCTCCTATATTTGGTTCATTCAGGAAAGCCATTACAGACATTGAATATGAGGGATTTTTCATTCCCAAGGGGTGGAAGGTCTTGTGGACGACGTATGGAACGCATCTCGAAGACGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCCCAGTATGTCTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAGCTGGCGAAATTGAACATCCTCATCTTTATACACTTGGTTGTAACTCGTTATGATTGGAGTTTAGTCCACCCAGATGAGACAATCATAATGGATCCCCTCCCTATCCCTTCTCATGGAATGCCAATTAAGATTTCTCCAAAATCAGAAGCTCGCCCCAATGTTCAGCAAATGTAA

Protein sequence

MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM
Homology
[Close]
BLAST of Cp4.1LG15g08580 vs. Swiss-Prot
Match: T5H_TAXCU (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata PE=1 SV=2)

HSP 1 Score: 348.2 bits (892), Expect = 1.3e-94
Identity = 181/443 (40.86%), Postives = 282/443 (63.66%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           +G+P+IGE+  F RA R N L E+F   RV K+G +FKTSL+G PTVV+ G   NR  LS
Sbjct: 57  LGIPFIGESFIFLRALRSNSL-EQFFDERVKKFGLVFKTSLIGHPTVVLCGPAGNRLILS 116

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NE KLV  SWP+  ++LMGE  +  ++GE H  +R  +A      +L++ + K+ T IQ 
Sbjct: 117 NEEKLVQMSWPAQFMKLMGENSVATRRGEDHIVMRSALAGFFGPGALQSYIGKMNTEIQS 176

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTI---VFECLMGIKVEPRMLTTFERVLEGVFAPPFSF 180
           +++ KW G D +++    + L F I   +F  +   + + R+    E +L G FA P   
Sbjct: 177 HINEKWKGKDEVNVLPLVRELVFNISAILFFNIYDKQEQDRLHKLLETILVGSFALPIDL 236

Query: 181 PGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGE-ISEVE 240
           PG  F RA + R ++ K++  ++++++  ++        +LL   +     +G  ++  E
Sbjct: 237 PGFGFHRALQGRAKLNKIMLSLIKKRKEDLQSGSATATQDLLSVLLTFRDDKGTPLTNDE 296

Query: 241 VVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIK 300
           ++DN   L+ A++DTT+  +A+ FK+L+  P CY  ++QE  +I+ +K  G+ +T +D+K
Sbjct: 297 ILDNFSSLLHASYDTTTSPMALIFKLLSSNPECYQKVVQEQLEILSNKEEGEEITWKDLK 356

Query: 301 KMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDP 360
            MKYTWQVA+E++R+FPP+FG+FRKAITDI+Y+G+ IPKGWK+LWTTY TH +D YF +P
Sbjct: 357 AMKYTWQVAQETLRMFPPVFGTFRKAITDIQYDGYTIPKGWKLLWTTYSTHPKDLYFNEP 416

Query: 361 LSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILIFI-HLVVTRYDWSLVHPD 420
             F PSRF++    V+ Y ++PFGGG R+C G++ +K+ IL+F+ H V T   ++ V PD
Sbjct: 417 EKFMPSRFDQEGKHVAPYTFLPFGGGQRSCVGWEFSKMEILLFVHHFVKTFSSYTPVDPD 476

Query: 421 ETIIMDPL-PIPSHGMPIKISPK 435
           E I  DPL P+PS G  IK+ P+
Sbjct: 477 EKISGDPLPPLPSKGFSIKLFPR 498

[Close]
BLAST of Cp4.1LG15g08580 vs. Swiss-Prot
Match: T13H_TAXCU (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata GN=CYP725A2 PE=1 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 5.4e-88
Identity = 170/444 (38.29%), Postives = 270/444 (60.81%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           +G P +GET+ F R+   +   ++F+  R++K+G +FKTS++G PTVV+ G   NR  LS
Sbjct: 43  LGFPLVGETLQFVRSLGSSTP-QQFIEERMSKFGDVFKTSIIGHPTVVLCGPAGNRLVLS 102

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NE KLV  SWPS+ ++L+GE+C+  K GE HR VR  +   L   +L+    K+ + IQ 
Sbjct: 103 NENKLVQMSWPSSMMKLIGEDCLGGKTGEQHRIVRAALTRFLGPQALQNHFAKMSSGIQR 162

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVE---PRMLTTFERVLEGVFAPPFSF 180
           +++ KW G D  ++    K L F++      GI  E    ++    E +L G F+ P + 
Sbjct: 163 HINEKWKGKDEATVLPLVKDLVFSVASRLFFGITEEHLQEQLHNLLEVILVGSFSVPLNI 222

Query: 181 PGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGE-ISEVE 240
           PG  + +A +AR  +  ++  ++ ++R  +      E  +LL   +     RG  +++ E
Sbjct: 223 PGFSYHKAIQARATLADIMTHLIEKRRNELRAGTASENQDLLSVLLTFTDERGNSLADKE 282

Query: 241 VVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIK 300
           ++DN  +L+  ++D+T+  + M  K+LA  P  Y  + QE   I+ +K  G+ +  +D+K
Sbjct: 283 ILDNFSMLLHGSYDSTNSPLTMLIKVLASHPESYEKVAQEQFGILSTKMEGEEIAWKDLK 342

Query: 301 KMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDP 360
           +MKY+WQV +E++R++PPIFG+FRKAITDI Y G+ IPKGWK+LWTTY T  ++EYF+D 
Sbjct: 343 EMKYSWQVVQETLRMYPPIFGTFRKAITDIHYNGYTIPKGWKLLWTTYSTQTKEEYFKDA 402

Query: 361 LSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD-WSLVHPD 420
             FKPSRFEE    V+ Y Y+PFGGG R C G++ AK+  L+F+H  V  +     + P+
Sbjct: 403 DQFKPSRFEEEGKHVTPYTYLPFGGGMRVCPGWEFAKMETLLFLHHFVKAFSGLKAIDPN 462

Query: 421 ETIIMDPL-PIPSHGMPIKISPKS 436
           E +   PL P+P +G+PIK+  +S
Sbjct: 463 EKLSGKPLPPLPVNGLPIKLYSRS 485

[Close]
BLAST of Cp4.1LG15g08580 vs. Swiss-Prot
Match: C16B2_PICSI (Cytochrome P450 716B2 OS=Picea sitchensis GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 9.2e-88
Identity = 176/437 (40.27%), Postives = 262/437 (59.95%), Query Frame = 1 

Query: 2   GLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSN 61
           G P IGET+ F   QRK     +F   R  KYG +F TSL+G PTVV    E NRF  SN
Sbjct: 57  GWPLIGETLEFLGCQRKGNP-RDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSN 116

Query: 62  EFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLY 121
           E KLVV+SWPS SV  +    ++   G+  + +R ++   L   +L   + ++ +  + +
Sbjct: 117 ENKLVVNSWPS-SVGNLFRSSLITTVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRH 176

Query: 122 LDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEP---RMLTTFERVLEGVFAPPFSFP 181
           L   W+G D ++     K  TF++  +    I  +    R+   F   ++GV   P   P
Sbjct: 177 LAEHWIGKDEVTALPLLKRYTFSLACDLFASINNKDDLGRLWLHFMVFVKGVMQIPIDLP 236

Query: 182 GTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGE-ISEVEV 241
           GTR+++AK A   I + L  ++ E++  +E      E +LL   +     +GE +++ E+
Sbjct: 237 GTRYNKAKHAANAIRQQLGSIINERKIGLEAGNASPEQDLLSFLLSNVDEQGESLTDNEI 296

Query: 242 VDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKK 301
            DN++LL++A HDT+S  + +  K LA+ P+CY  +L+E  DI  SK AGQ +  ED+++
Sbjct: 297 QDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQR 356

Query: 302 MKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPL 361
           MKY+W+VA+E++RLFP   GSFRKAI +  Y+GF IPKGWK+ WT   TH + EYF +P 
Sbjct: 357 MKYSWRVAQEALRLFPAAQGSFRKAIKEFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPE 416

Query: 362 SFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETII 421
           +F PSRFE E    Y +VPFGGGPR C G + A+L IL+F+H +V    W+LV+P E +I
Sbjct: 417 TFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARLEILVFLHNIVKNCKWNLVNPGEKVI 476

Query: 422 MDPLPIPSHGMPIKISP 434
           +DP+P P +G+PIK+ P
Sbjct: 477 VDPMPAPVNGLPIKLVP 491

[Close]
BLAST of Cp4.1LG15g08580 vs. Swiss-Prot
Match: C16B1_PICSI (Cytochrome P450 716B1 OS=Picea sitchensis GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 7.8e-87
Identity = 173/437 (39.59%), Postives = 261/437 (59.73%), Query Frame = 1 

Query: 2   GLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSN 61
           G P IGET+ F   QRK     +F   R  KYG +F TSL+G PTVV    E NRF  SN
Sbjct: 57  GWPLIGETLEFLGCQRKGNP-RDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSN 116

Query: 62  EFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLY 121
           E KLVV+SWPS SV  +    ++   G+  + +R ++   L   +L   + ++ +  + +
Sbjct: 117 ENKLVVNSWPS-SVGNLFRSSLITTVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRH 176

Query: 122 LDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEP---RMLTTFERVLEGVFAPPFSFP 181
           L   W+G D +      K  TF++  +    I  +    R+   F   ++GV   P   P
Sbjct: 177 LAEHWIGKDEVMALPLLKRYTFSLACDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLP 236

Query: 182 GTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGE-ISEVEV 241
           GTR+++ K A   I + L  ++ E++  +E      E +LL   +     +GE +++ E+
Sbjct: 237 GTRYNKTKHAANAIRQQLGSIINERKIALEAGNASPEQDLLSFLLSNVDEQGESLTDNEI 296

Query: 242 VDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKK 301
            DN++LL++A HDT+S  + +  K LA+ P+CY  +L+E  +I  SK  GQ +  ED+++
Sbjct: 297 QDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQR 356

Query: 302 MKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPL 361
           MKY+W+VA+E++RLFP + GSFRKAI +  Y+GF IPKGWK+ WT   TH + EYF +P 
Sbjct: 357 MKYSWRVAQEALRLFPAVQGSFRKAIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPE 416

Query: 362 SFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETII 421
            F PSRFE E    Y +VPFGGGPR C G + A++ ILIF+H +V  ++W+LV+P E +I
Sbjct: 417 KFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARMEILIFLHNIVKNFNWNLVNPLEKVI 476

Query: 422 MDPLPIPSHGMPIKISP 434
           +DP+P P +G+PIK+ P
Sbjct: 477 VDPMPAPVNGLPIKLVP 491

[Close]
BLAST of Cp4.1LG15g08580 vs. Swiss-Prot
Match: T14H_TAXCU (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 8.6e-86
Identity = 173/444 (38.96%), Postives = 267/444 (60.14%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           +G P+IGE++ F +A R N + E+F+  RV  +G +FKTSL+G PTVV+ G   NR  L+
Sbjct: 55  LGYPFIGESLLFLKALRSNTV-EQFLDERVKNFGNVFKTSLIGHPTVVLCGPAGNRLILA 114

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NE KLV  SWP +S++LMGE+ I  K+GE H  +R  +    +  +L+  + ++  TI+ 
Sbjct: 115 NEEKLVQMSWPKSSMKLMGEKSITAKRGEGHMIIRSALQGFFSPGALQKYIGQMSKTIEN 174

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVE---PRMLTTFERVLEGVFAPPFSF 180
           +++ KW G+D +S+      L F I       I  +    R+    E +  GV A P   
Sbjct: 175 HINEKWKGNDQVSVVALVGDLVFDISACLFFNINEKHERERLFELLEIIAVGVLAVPVDL 234

Query: 181 PGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGE-ISEVE 240
           PG  + RA +AR ++  +L  ++ +++  +         +LL   +     RG   S+ E
Sbjct: 235 PGFAYHRALQARSKLNAILSGLIEKRKMDLSSGLATSNQDLLSVFLTFKDDRGNPCSDEE 294

Query: 241 VVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIK 300
           ++DN   L+  ++DTT  A+A  FK+L+  P CY  ++QE   I+ +K  G  +T +D+K
Sbjct: 295 ILDNFSGLLHGSYDTTVSAMACVFKLLSSNPECYEKVVQEQLGILSNKLEGDEITWKDVK 354

Query: 301 KMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDP 360
            MKYTWQV +E++RL+P IFGSFR+AITDI Y G+ IPKGWK+LWT Y TH ++ YF +P
Sbjct: 355 SMKYTWQVVQETLRLYPSIFGSFRQAITDIHYNGYIIPKGWKLLWTPYTTHPKEMYFSEP 414

Query: 361 LSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILIFI-HLVVTRYDWSLVHPD 420
             F PSRF++    V+ Y ++PFGGG R+C G++ +K+ IL+ + H V T   ++ V P 
Sbjct: 415 EKFLPSRFDQEGKLVAPYTFLPFGGGQRSCPGWEFSKMEILLSVHHFVKTFSTFTPVDPA 474

Query: 421 ETIIMDPL-PIPSHGMPIKISPKS 436
           E I  D L P+PS+G  +K+ P+S
Sbjct: 475 EIIARDSLCPLPSNGFSVKLFPRS 497

[Close]
BLAST of Cp4.1LG15g08580 vs. TrEMBL
Match: A0A0A0KEB2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G423370 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 2.2e-221
Identity = 381/444 (85.81%), Postives = 410/444 (92.34%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 1   MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HRCVRGLIAA+L++SSL+ MMPKLC TIQ+
Sbjct: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV 120

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           YLDTKW+G D I L+HSAKVLTFTIVFECL+GI+VEPR+LT FERVLEGVFAPPF FPGT
Sbjct: 121 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT 180

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDN 240
           RFSRAKKAR+EI   LREVVREKRR+IE + EEEEC  LLSRMVRAMI GEISE+EV+DN
Sbjct: 181 RFSRAKKARLEIETTLREVVREKRRKIEGKLEEEECGSLLSRMVRAMIGGEISEMEVIDN 240

Query: 241 VVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKY 300
           +VLLVFAAHDTTSFAIAMTFKML+Q  NCY+LLLQEH DI+RSKG GQN+TMEDIKKMKY
Sbjct: 241 IVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKY 300

Query: 301 TWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFK 360
           TWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH  +EYFEDPLSFK
Sbjct: 301 TWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFK 360

Query: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPL 420
           PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF+H VVTRYDWSLVHPDE IIMDPL
Sbjct: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPL 420

Query: 421 PIPSHGMPIKISPKSEARPNVQQM 445
           P+P HGMPIKISPKS+   NV QM
Sbjct: 421 PVPYHGMPIKISPKSQVCSNVHQM 444

[Close]
BLAST of Cp4.1LG15g08580 vs. TrEMBL
Match: V4TFN7_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031591mg PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 2.7e-187
Identity = 321/434 (73.96%), Postives = 376/434 (86.64%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLP+IGET+ F++AQR NRLF++FV PRV KYGKIFKT L+GSPTVVVNGAEANRFFLS
Sbjct: 1   MGLPFIGETIEFFKAQRNNRLFDDFVQPRVTKYGKIFKTRLLGSPTVVVNGAEANRFFLS 60

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLV+SSWPS+SVQLMG E IMQKQGE HRC+RG++A+ L ++ L+A++PK+C ++QL
Sbjct: 61  NEFKLVISSWPSSSVQLMGNESIMQKQGEQHRCIRGILASCLHNAGLDALVPKICNSVQL 120

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           +LDT W G D +SLY S K+LTFTIVFECL+GI+VEP ML TFERVLEGVFAP   FPG+
Sbjct: 121 HLDTHWHGQDSLSLYRSTKILTFTIVFECLLGIRVEPGMLNTFERVLEGVFAPAIKFPGS 180

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDN 240
           RFSRAKKAR EI KML +VVREKR  +E   E+E   +LLS++V  MIRG+I+E EV DN
Sbjct: 181 RFSRAKKARQEIEKMLVKVVREKRNEMEFGNEQEG--MLLSQLVAGMIRGDITEAEVTDN 240

Query: 241 VVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKY 300
           VVLLVFAAHDTTSFAIAMTFKMLAQ P+CYSLLLQEH +I+ +K AG+ +T+ED+KK+KY
Sbjct: 241 VVLLVFAAHDTTSFAIAMTFKMLAQHPDCYSLLLQEHVNIMNNKRAGEILTLEDMKKIKY 300

Query: 301 TWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFK 360
           TWQVARES+RLFPPIFGSFRKA++DIEYEGF IPKGWKVLWT +GTH   EYFEDPLSF 
Sbjct: 301 TWQVARESMRLFPPIFGSFRKAVSDIEYEGFTIPKGWKVLWTAFGTHSNPEYFEDPLSFD 360

Query: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPL 420
           P RFEE V  YVY+PFGGGPR CAGYQLAKLNI+IF+H VVTRYDWSL++P+E+I MDPL
Sbjct: 361 PRRFEESVPPYVYLPFGGGPRLCAGYQLAKLNIIIFVHYVVTRYDWSLINPNESITMDPL 420

Query: 421 PIPSHGMPIKISPK 435
           P PS GMPIKISPK
Sbjct: 421 PFPSQGMPIKISPK 432

[Close]
BLAST of Cp4.1LG15g08580 vs. TrEMBL
Match: M5WZ42_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017753mg PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 2.3e-186
Identity = 323/436 (74.08%), Postives = 371/436 (85.09%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLPWIG+TM FY+AQRKN LF++F+ PRV KYGKIF T LMGSPTV+VNGA AN+F LS
Sbjct: 1   MGLPWIGQTMEFYKAQRKNSLFDDFIQPRVTKYGKIFSTHLMGSPTVIVNGANANKFLLS 60

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLVVSSWPSASVQLMG++ IM+KQGE HRC+RGLI +SL  + LE ++PK+C ++QL
Sbjct: 61  NEFKLVVSSWPSASVQLMGKDSIMEKQGERHRCLRGLIGSSLGQAGLETLVPKICNSVQL 120

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           +LDTKW G D ISLY S KVLTFTIVFECL+G KVE  ML TFERVLEGVFA P   PG+
Sbjct: 121 HLDTKWKGQDKISLYRSTKVLTFTIVFECLLGNKVEAEMLETFERVLEGVFAAPVMIPGS 180

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEE--EECELLLSRMVRAMIRGEISEVEVV 240
           RF RAKKAR  I KML EVVREKR ++E + EE  E+  +LLSR+V  MIRGEI++ EVV
Sbjct: 181 RFWRAKKARKTIEKMLVEVVREKRMQMEGKLEEQGEQGGMLLSRLVAGMIRGEITKEEVV 240

Query: 241 DNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKM 300
           DNVVLLVFAAHDTTSFAIAMTFKMLAQ P C+SLLL+EHA+I+RSK  G+N+T++D KKM
Sbjct: 241 DNVVLLVFAAHDTTSFAIAMTFKMLAQNPYCHSLLLKEHAEIMRSKRPGENLTLDDTKKM 300

Query: 301 KYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLS 360
           KYTWQVARES+RLFPPIFGSFRKAI DIEYEGF IP+GWKVLWTTYGTH   EYF+DPL 
Sbjct: 301 KYTWQVARESMRLFPPIFGSFRKAIADIEYEGFTIPRGWKVLWTTYGTHYNSEYFQDPLR 360

Query: 361 FKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMD 420
           F PSRF+E V  YV++ FGGGPR CAGYQLAKLNILIF+H VVTRYDWSL++PDE + MD
Sbjct: 361 FNPSRFDESVPPYVFLAFGGGPRVCAGYQLAKLNILIFVHFVVTRYDWSLLYPDEPVTMD 420

Query: 421 PLPIPSHGMPIKISPK 435
           PLP PSHGMPIKI+PK
Sbjct: 421 PLPFPSHGMPIKITPK 436

[Close]
BLAST of Cp4.1LG15g08580 vs. TrEMBL
Match: A0A067FBT4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g035672mg PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 8.7e-186
Identity = 318/434 (73.27%), Postives = 374/434 (86.18%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MG P+IGET+ F++AQR NRLF++FV PRV KYGKIFKT L+GSPTVVVNGAEANRFFLS
Sbjct: 1   MGHPFIGETIEFFKAQRNNRLFDDFVQPRVTKYGKIFKTRLLGSPTVVVNGAEANRFFLS 60

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLV+SSWPS+SVQLMG E IMQKQGE HRC+RG++A+ L ++ L+A++PK+C ++QL
Sbjct: 61  NEFKLVISSWPSSSVQLMGNESIMQKQGEQHRCIRGILASCLHNAGLDALVPKICNSVQL 120

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           +LD  W G D +SLY S K+LTFTIVFECL+GI+VEP ML TFERVLEGVFAP   FPG+
Sbjct: 121 HLDAHWHGQDSLSLYRSTKILTFTIVFECLLGIRVEPGMLNTFERVLEGVFAPAIKFPGS 180

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDN 240
           RFSRAKKAR EI KML +VVREKR  +E   E+E   +LLS++V  MIRG+I+E EV DN
Sbjct: 181 RFSRAKKARQEIEKMLVKVVREKRNEMEFGNEQEG--MLLSQLVAGMIRGDITEAEVTDN 240

Query: 241 VVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKY 300
           +VLLVFAAHDTTSFAIAMTFKMLAQ P+CYSLLLQEH +I+ +K AG+ +T+ED+KK+KY
Sbjct: 241 IVLLVFAAHDTTSFAIAMTFKMLAQHPDCYSLLLQEHVNIMNNKRAGEILTLEDMKKIKY 300

Query: 301 TWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFK 360
           TWQVARES+RLFPPIFGSFRKA++DIEYEGF IPKGWKVLWT +GTH   EYFEDPLSF 
Sbjct: 301 TWQVARESMRLFPPIFGSFRKAVSDIEYEGFTIPKGWKVLWTAFGTHSNPEYFEDPLSFD 360

Query: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPL 420
           P RFEE V  YVY+PFGGGPR CAGYQLAKLNI+IF+H VVTRYDWSL++P+E+I MDPL
Sbjct: 361 PRRFEESVPPYVYLPFGGGPRLCAGYQLAKLNIIIFVHYVVTRYDWSLINPNESITMDPL 420

Query: 421 PIPSHGMPIKISPK 435
           P PS GMPIKISPK
Sbjct: 421 PFPSQGMPIKISPK 432

[Close]
BLAST of Cp4.1LG15g08580 vs. TrEMBL
Match: B9GT44_POPTR (Cytochrome P450 family protein OS=Populus trichocarpa GN=POPTR_0002s06150g PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 2.1e-184
Identity = 320/435 (73.56%), Postives = 367/435 (84.37%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGET+ FYR+QR N+LFEEFV PR+ KYGKIFKT LMGSPT++VNGAEANRFFLS
Sbjct: 25  MGLPWIGETIEFYRSQRDNQLFEEFVQPRITKYGKIFKTRLMGSPTIIVNGAEANRFFLS 84

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLVVSSWP+ASVQLMG   IM+KQGE HRC+RG+IA SL  + LE ++PK+C ++QL
Sbjct: 85  NEFKLVVSSWPTASVQLMGINSIMEKQGEKHRCLRGIIATSLGPAGLEILVPKICDSVQL 144

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           YLD  W   + ISLYHS K LTFTIV ECL+G+  EP  L TFERVLEGVFAPP SFPG+
Sbjct: 145 YLDKNWNVREEISLYHSTKALTFTIVLECLLGLNFEPGTLNTFERVLEGVFAPPISFPGS 204

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDN 240
           +FSRAKKAR EI +ML +VVREKR+++E     +E  +L S++V  MIRGEISE EVVDN
Sbjct: 205 KFSRAKKARREIKEMLIKVVREKRKKMESGLGGDE-GMLFSQLVSGMIRGEISEEEVVDN 264

Query: 241 VVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKY 300
           VVLLVFAAHDTTSFAIAMTFKMLA+ P+C+SLLLQEH DI+  + +G+N+TMED KKMKY
Sbjct: 265 VVLLVFAAHDTTSFAIAMTFKMLAEHPDCHSLLLQEHDDIMNKRRSGENLTMEDTKKMKY 324

Query: 301 TWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFK 360
           TWQVARES+RLFPPIFGSFRKAI DIEYEGF IPKGWKVLWTTYGTH  +EYF+DPL+F 
Sbjct: 325 TWQVARESMRLFPPIFGSFRKAIADIEYEGFTIPKGWKVLWTTYGTHYNEEYFKDPLTFN 384

Query: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPL 420
           P RFEEP+  Y Y+PFGGGPR CAG QLAKLNILIFIH VVTRY+WSL+ P E I MDPL
Sbjct: 385 PRRFEEPIPPYAYLPFGGGPRLCAGNQLAKLNILIFIHYVVTRYNWSLLCPGEQITMDPL 444

Query: 421 PIPSHGMPIKISPKS 436
           P PSHGMPIK+S KS
Sbjct: 445 PFPSHGMPIKVSLKS 458

[Close]
BLAST of Cp4.1LG15g08580 vs. TAIR10
Match: AT2G42850.1 (AT2G42850.1 cytochrome P450, family 718)

HSP 1 Score: 601.7 bits (1550), Expect = 3.7e-172
Identity = 289/434 (66.59%), Postives = 353/434 (81.34%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FY+AQ+ NR+FE+FV PR+ K+G IFKT +MGSPT+VVNGAEANR  LS
Sbjct: 50  MGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKHGNIFKTRIMGSPTIVVNGAEANRLILS 109

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEF LVVSSWPS+SVQLMG  CIM KQGE HR +RG++A SL+   LE+++PKLC T++ 
Sbjct: 110 NEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKF 169

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           + +T+W G + ISLY SAKVLTFT+VFECL GIKVE  ML  FERVLEGVFA P  FP +
Sbjct: 170 HHETEWRGKEEISLYRSAKVLTFTVVFECLYGIKVEIGMLEVFERVLEGVFALPVEFPCS 229

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDN 240
           +F+RAKKAR+EI   L   VREKRR +E+E  E+    L SR+V  +I+G I+E EVVDN
Sbjct: 230 KFARAKKARLEIETFLVGKVREKRREMEKEGAEKPNTTLFSRLVEELIKGVITEEEVVDN 289

Query: 241 VVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKY 300
           +VLLVFAAHDTTS+A++MTFKMLAQ P C   LLQEHA I  +KG G+ +T+ED+KKMKY
Sbjct: 290 MVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKY 349

Query: 301 TWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFK 360
           +WQV RE++RL PPIFGSFRKA+ DI+Y G+ IPKGWK+LWTTYGTH   E F+DP+SF 
Sbjct: 350 SWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFD 409

Query: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPL 420
           P+RF++P+  Y Y+PFGGGPR CAG+QLAK++IL+F+H VVT +DWSLV+PDETI MDPL
Sbjct: 410 PTRFDKPIQAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPL 469

Query: 421 PIPSHGMPIKISPK 435
           P PS GMPIKISPK
Sbjct: 470 PFPSLGMPIKISPK 483

[Close]
BLAST of Cp4.1LG15g08580 vs. TAIR10
Match: AT5G36110.1 (AT5G36110.1 cytochrome P450, family 716, subfamily A, polypeptide 1)

HSP 1 Score: 299.3 bits (765), Expect = 4.0e-81
Identity = 164/443 (37.02%), Postives = 252/443 (56.88%), Query Frame = 1 

Query: 2   GLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGK----IFKTSLMGSPTVVVNGAEANRF 61
           GLP IGE+ +F  A R+    E+F+  RV ++      +FKT L GSPT VV GA  N+F
Sbjct: 40  GLPLIGESFSFLSAGRQGHP-EKFITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKF 99

Query: 62  FLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTT 121
             +NE KLVVS WP  SV  +    +     E  R +R L++  +   +L   +  +   
Sbjct: 100 LFTNENKLVVSWWPD-SVNKIFPSSMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEI 159

Query: 122 IQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTT---FERVLEGVFAPP 181
            Q + +T+W   D + ++   K  TF+I     + ++   R+      F  V  G+F+ P
Sbjct: 160 AQRHFETEWANQDQVIVFPLTKKFTFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIP 219

Query: 182 FSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISE 241
              PGTRF+RA KA   + K +  +VR+++  ++     EE ++L   ++     GE  +
Sbjct: 220 IDLPGTRFNRAIKASRLLRKEVSAIVRQRKEELKAGKALEEHDILSHMLMNI---GETKD 279

Query: 242 VEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMED 301
            ++ D ++ L+   HDT S         LA+ P+ Y  +LQE  +I++ K   + +  ED
Sbjct: 280 EDLADKIIGLLIGGHDTASIVCTFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWED 339

Query: 302 IKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFE 361
           I+KM+Y+W VA E +R+ PP+ G+FR+AI    ++GF+IPKGWK+ W+   TH+  +YF 
Sbjct: 340 IEKMRYSWNVACEVMRIVPPLSGTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFP 399

Query: 362 DPLSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDE 421
           +P  F+P+RFE      Y YVPFGGGPR C G + A+L ILIF+H +V R+ W  V P+E
Sbjct: 400 EPERFEPNRFEGSGPKPYTYVPFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNE 459

Query: 422 T-IIMDPLPIPSHGMPIKISPKS 436
             I++DPLPIP  G+PI+I P+S
Sbjct: 460 NKIVVDPLPIPDKGLPIRIFPQS 477

[Close]
BLAST of Cp4.1LG15g08580 vs. TAIR10
Match: AT2G29090.1 (AT2G29090.1 cytochrome P450, family 707, subfamily A, polypeptide 2)

HSP 1 Score: 242.7 bits (618), Expect = 4.4e-64
Identity = 144/438 (32.88%), Postives = 230/438 (52.51%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLP+IGET+  Y     +     F   R  KYG IFKT ++G P V+++  EA R  L 
Sbjct: 54  MGLPYIGETLRLYTENPNS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLV 113

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           ++  L   ++P +  +++G E +   QG  H  ++ L+ +S   S+L   +  +   + L
Sbjct: 114 SKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHI-ELLVL 173

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTT----FERVLEGVFAPPFS 180
              + W     I+     K   F +      G K EP  +      ++R+  G  + P  
Sbjct: 174 QTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLD 233

Query: 181 FPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVE 240
            PGT F ++ KAR+E+ + LR+V+ EKRR   E   EE   L +    +   R  +S+ +
Sbjct: 234 LPGTLFHKSMKARIELSEELRKVI-EKRR---ENGREEGGLLGVLLGAKDQKRNGLSDSQ 293

Query: 241 VVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRS-KGAGQNMTMEDI 300
           + DN++ ++FAA DTT+  +    K L   PN    + +E   I +  K   + ++ ED 
Sbjct: 294 IADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDT 353

Query: 301 KKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFED 360
           +KM  T +V +E++R    +  +FR+A+ D+EY+G+ IPKGWKVL      H   E+F D
Sbjct: 354 RKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPD 413

Query: 361 PLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETI 420
           P  F PSRFE     Y Y+PFG G  +C G +LAKL +LI +H + T + W ++  +E I
Sbjct: 414 PEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGI 473

Query: 421 IMDPLPIPSHGMPIKISP 434
              P P+P  G+PI+++P
Sbjct: 474 QYGPFPVPKKGLPIRVTP 481

[Close]
BLAST of Cp4.1LG15g08580 vs. TAIR10
Match: AT5G45340.1 (AT5G45340.1 cytochrome P450, family 707, subfamily A, polypeptide 3)

HSP 1 Score: 237.3 bits (604), Expect = 1.9e-62
Identity = 137/437 (31.35%), Postives = 235/437 (53.78%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MG P++GET   Y +Q  N  F      +  +YG +FKT ++G P V+++  EA +F L 
Sbjct: 41  MGYPYVGETFQLY-SQDPNVFF----AAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLV 100

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
            +  L   ++P++  +++G++ I   QG+ H  +R L+  +    ++  M+P + +  Q 
Sbjct: 101 TKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQE 160

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMG---IKVEPRMLTTFERVLEGVFAPPFSF 180
            L++ W G  + + Y   K  TF +    ++G   +     +   +  + +G  + P + 
Sbjct: 161 SLNS-WDGTQL-NTYQEMKTYTFNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINL 220

Query: 181 PGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEV 240
           PGT F +A KAR E+ ++L  ++ ++R+           +LL S M     +  +++ ++
Sbjct: 221 PGTLFHKAMKARKELAQILANILSKRRQN-----PSSHTDLLGSFMED---KAGLTDEQI 280

Query: 241 VDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKK 300
            DN++ ++FAA DTT+  +    K LA  P     + +E   I + K  G+++T ED KK
Sbjct: 281 ADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKK 340

Query: 301 MKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPL 360
           M  T++V +E++R    +  +FR+A+ D+EYEG+ IPKGWKVL      H   + F DP 
Sbjct: 341 MPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPG 400

Query: 361 SFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIM 420
            F PSRFE       ++PFG G  +C G +LAKL I + IH + T+Y WS+V P + I  
Sbjct: 401 KFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQY 460

Query: 421 DPLPIPSHGMPIKISPK 435
            P  +P +G+PI +  K
Sbjct: 461 GPFALPQNGLPIALERK 462

[Close]
BLAST of Cp4.1LG15g08580 vs. TAIR10
Match: AT3G50660.1 (AT3G50660.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 220.3 bits (560), Expect = 2.3e-57
Identity = 141/468 (30.13%), Postives = 241/468 (51.50%), Query Frame = 1 

Query: 2   GLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSN 61
           G P++GET+ + +      L  +F+   V+KYGKI++++L G PT+V   A  NRF L N
Sbjct: 45  GWPFLGETIGYLKPYTATTL-GDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNRFILQN 104

Query: 62  EFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLY 121
           E +L   S+P +   ++G+  ++   G+ HR +R +    L+ + L  ++ K      L+
Sbjct: 105 EGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVERHTLF 164

Query: 122 LDTKWLGHDIISLYHSAKVLTFTIVFECLM----GIKVEPRMLTTFERVLEGVFAPPFSF 181
           +   W  + I S    AK  TF ++ + +M    G +   ++   +   ++GV + P + 
Sbjct: 165 VLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVSAPLNL 224

Query: 182 PGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEE-------ECELLLSRMVR----- 241
           PGT + +A ++R  I K +   + E++  I+EE +EE       E E+  S  VR     
Sbjct: 225 PGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTD 284

Query: 242 ------AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHAD 301
                  +    +S  +++D ++ L+FA H+T+S AIA+    L   P     L +EH +
Sbjct: 285 DDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEELREEHLE 344

Query: 302 IVRSKG--AGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGW 361
           I R+K       +  +D KKM +T  V  E++RL   +    RKA+ D+ Y+G+ IP GW
Sbjct: 345 IARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGW 404

Query: 362 KVLWTTYGTHLEDEYFEDPLSFKPSRFEEPVSQYV-------------YVPFGGGPRACA 421
           KVL      HL++  ++ P  F P R+++  +                Y+PFGGGPR CA
Sbjct: 405 KVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSGSFSTWGNNYMPFGGGPRLCA 464

Query: 422 GYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKIS 433
           G +LAKL + +FIH +V +++W L   D+      +  P +G+PI++S
Sbjct: 465 GSELAKLEMAVFIHHLVLKFNWELAEDDKPFAFPFVDFP-NGLPIRVS 510

[Close]
BLAST of Cp4.1LG15g08580 vs. NCBI nr
Match: gi|659097243|ref|XP_008449518.1| (PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis melo])

HSP 1 Score: 778.5 bits (2009), Expect = 6.3e-222
Identity = 388/445 (87.19%), Postives = 412/445 (92.58%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETMAFYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 32  MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 91

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HRCVRGLIA++L++SSLE MMPKLC TIQ+
Sbjct: 92  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 151

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           YLDTKW+  D + L+ SAKVLTFTIVFECL+GIKVEPR+LTTFERVLEGVFAPPF FPGT
Sbjct: 152 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIKVEPRVLTTFERVLEGVFAPPFRFPGT 211

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEE-CELLLSRMVRAMIRGEISEVEVVD 240
           RFSRAKKAR+EI  MLREVVREKRR+IE + EEEE C  LLSRMVRAMI GEISE+EVVD
Sbjct: 212 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 271

Query: 241 NVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMK 300
           N+VLLVFAAHDTTSFAIAMTFKML+Q  NCYSLLLQEHADIVRSKG GQN+TMEDIKKMK
Sbjct: 272 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 331

Query: 301 YTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH  +EYF DPLSF
Sbjct: 332 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 391

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSLVHPDE IIMDP
Sbjct: 392 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 451

Query: 421 LPIPSHGMPIKISPKSEARPNVQQM 445
           LP+P HGMPIKISPKSE  PNV QM
Sbjct: 452 LPVPCHGMPIKISPKSEICPNVHQM 476

[Close]
BLAST of Cp4.1LG15g08580 vs. NCBI nr
Match: gi|449444975|ref|XP_004140249.1| (PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis sativus])

HSP 1 Score: 776.2 bits (2003), Expect = 3.1e-221
Identity = 381/444 (85.81%), Postives = 410/444 (92.34%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 32  MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 91

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HRCVRGLIAA+L++SSL+ MMPKLC TIQ+
Sbjct: 92  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV 151

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           YLDTKW+G D I L+HSAKVLTFTIVFECL+GI+VEPR+LT FERVLEGVFAPPF FPGT
Sbjct: 152 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT 211

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDN 240
           RFSRAKKAR+EI   LREVVREKRR+IE + EEEEC  LLSRMVRAMI GEISE+EV+DN
Sbjct: 212 RFSRAKKARLEIETTLREVVREKRRKIEGKLEEEECGSLLSRMVRAMIGGEISEMEVIDN 271

Query: 241 VVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKY 300
           +VLLVFAAHDTTSFAIAMTFKML+Q  NCY+LLLQEH DI+RSKG GQN+TMEDIKKMKY
Sbjct: 272 IVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKY 331

Query: 301 TWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFK 360
           TWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH  +EYFEDPLSFK
Sbjct: 332 TWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFK 391

Query: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPL 420
           PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF+H VVTRYDWSLVHPDE IIMDPL
Sbjct: 392 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPL 451

Query: 421 PIPSHGMPIKISPKSEARPNVQQM 445
           P+P HGMPIKISPKS+   NV QM
Sbjct: 452 PVPYHGMPIKISPKSQVCSNVHQM 475

[Close]
BLAST of Cp4.1LG15g08580 vs. NCBI nr
Match: gi|700192791|gb|KGN47995.1| (hypothetical protein Csa_6G423370 [Cucumis sativus])

HSP 1 Score: 776.2 bits (2003), Expect = 3.1e-221
Identity = 381/444 (85.81%), Postives = 410/444 (92.34%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 1   MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HRCVRGLIAA+L++SSL+ MMPKLC TIQ+
Sbjct: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV 120

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           YLDTKW+G D I L+HSAKVLTFTIVFECL+GI+VEPR+LT FERVLEGVFAPPF FPGT
Sbjct: 121 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT 180

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDN 240
           RFSRAKKAR+EI   LREVVREKRR+IE + EEEEC  LLSRMVRAMI GEISE+EV+DN
Sbjct: 181 RFSRAKKARLEIETTLREVVREKRRKIEGKLEEEECGSLLSRMVRAMIGGEISEMEVIDN 240

Query: 241 VVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKY 300
           +VLLVFAAHDTTSFAIAMTFKML+Q  NCY+LLLQEH DI+RSKG GQN+TMEDIKKMKY
Sbjct: 241 IVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKY 300

Query: 301 TWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFK 360
           TWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH  +EYFEDPLSFK
Sbjct: 301 TWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFK 360

Query: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPL 420
           PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF+H VVTRYDWSLVHPDE IIMDPL
Sbjct: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPL 420

Query: 421 PIPSHGMPIKISPKSEARPNVQQM 445
           P+P HGMPIKISPKS+   NV QM
Sbjct: 421 PVPYHGMPIKISPKSQVCSNVHQM 444

[Close]
BLAST of Cp4.1LG15g08580 vs. NCBI nr
Match: gi|645246652|ref|XP_008229454.1| (PREDICTED: taxadiene 5-alpha hydroxylase [Prunus mume])

HSP 1 Score: 662.9 bits (1709), Expect = 3.9e-187
Identity = 322/436 (73.85%), Postives = 374/436 (85.78%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLPWIG+TM FY+AQRKNRLF++FV PR+ KYGKIF T LMGSPTV+VNGA AN+F LS
Sbjct: 44  MGLPWIGQTMEFYKAQRKNRLFDDFVQPRITKYGKIFSTHLMGSPTVIVNGANANKFLLS 103

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLVVSSWPSASVQLMG++ IM+KQGE HRC+RGLI +SL  + LE ++PK+C ++QL
Sbjct: 104 NEFKLVVSSWPSASVQLMGKDSIMEKQGERHRCLRGLIGSSLGQAGLETLVPKICNSVQL 163

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           +LDTKW G D ISLYHS K+LTFTIVFECL+GIKVE  ML TFERVLEGVFA P   PG+
Sbjct: 164 HLDTKWKGQDKISLYHSTKILTFTIVFECLLGIKVEAEMLETFERVLEGVFAAPVMIPGS 223

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECE--LLLSRMVRAMIRGEISEVEVV 240
            F RAKKAR  I KML EVVREKR ++E + EE+E +  +LLSR+V  MIRGEI++ EVV
Sbjct: 224 SFWRAKKARKTIEKMLVEVVREKRMQMEGKLEEQEEQGGMLLSRLVAGMIRGEITKEEVV 283

Query: 241 DNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKM 300
           DNVVLLVFAAHDTTSFAIAMTFKMLAQ P C+SLLL+EHA+I+RSK  G+N+T++D KK+
Sbjct: 284 DNVVLLVFAAHDTTSFAIAMTFKMLAQNPYCHSLLLKEHAEIMRSKRPGENLTLDDTKKL 343

Query: 301 KYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLS 360
           KYTWQVARES+RLFPPIFGSFRKAI DIEYEGF IP+GWKVLWTTYGTH   EYF+DPLS
Sbjct: 344 KYTWQVARESMRLFPPIFGSFRKAIADIEYEGFTIPRGWKVLWTTYGTHYNSEYFQDPLS 403

Query: 361 FKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMD 420
           F PSRF+E V  YV++ FGGGPR CAGYQLAKLNILIF+H VVT YDWSL++PDE + MD
Sbjct: 404 FNPSRFDESVPPYVFLAFGGGPRLCAGYQLAKLNILIFVHFVVTLYDWSLLYPDEPVTMD 463

Query: 421 PLPIPSHGMPIKISPK 435
           PLP PSHGMPIK++PK
Sbjct: 464 PLPFPSHGMPIKMTPK 479

[Close]
BLAST of Cp4.1LG15g08580 vs. NCBI nr
Match: gi|567889346|ref|XP_006437195.1| (hypothetical protein CICLE_v10031591mg [Citrus clementina])

HSP 1 Score: 662.9 bits (1709), Expect = 3.9e-187
Identity = 321/434 (73.96%), Postives = 376/434 (86.64%), Query Frame = 1 

Query: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60
           MGLP+IGET+ F++AQR NRLF++FV PRV KYGKIFKT L+GSPTVVVNGAEANRFFLS
Sbjct: 1   MGLPFIGETIEFFKAQRNNRLFDDFVQPRVTKYGKIFKTRLLGSPTVVVNGAEANRFFLS 60

Query: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120
           NEFKLV+SSWPS+SVQLMG E IMQKQGE HRC+RG++A+ L ++ L+A++PK+C ++QL
Sbjct: 61  NEFKLVISSWPSSSVQLMGNESIMQKQGEQHRCIRGILASCLHNAGLDALVPKICNSVQL 120

Query: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180
           +LDT W G D +SLY S K+LTFTIVFECL+GI+VEP ML TFERVLEGVFAP   FPG+
Sbjct: 121 HLDTHWHGQDSLSLYRSTKILTFTIVFECLLGIRVEPGMLNTFERVLEGVFAPAIKFPGS 180

Query: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDN 240
           RFSRAKKAR EI KML +VVREKR  +E   E+E   +LLS++V  MIRG+I+E EV DN
Sbjct: 181 RFSRAKKARQEIEKMLVKVVREKRNEMEFGNEQEG--MLLSQLVAGMIRGDITEAEVTDN 240

Query: 241 VVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKY 300
           VVLLVFAAHDTTSFAIAMTFKMLAQ P+CYSLLLQEH +I+ +K AG+ +T+ED+KK+KY
Sbjct: 241 VVLLVFAAHDTTSFAIAMTFKMLAQHPDCYSLLLQEHVNIMNNKRAGEILTLEDMKKIKY 300

Query: 301 TWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFK 360
           TWQVARES+RLFPPIFGSFRKA++DIEYEGF IPKGWKVLWT +GTH   EYFEDPLSF 
Sbjct: 301 TWQVARESMRLFPPIFGSFRKAVSDIEYEGFTIPKGWKVLWTAFGTHSNPEYFEDPLSFD 360

Query: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPL 420
           P RFEE V  YVY+PFGGGPR CAGYQLAKLNI+IF+H VVTRYDWSL++P+E+I MDPL
Sbjct: 361 PRRFEESVPPYVYLPFGGGPRLCAGYQLAKLNIIIFVHYVVTRYDWSLINPNESITMDPL 420

Query: 421 PIPSHGMPIKISPK 435
           P PS GMPIKISPK
Sbjct: 421 PFPSQGMPIKISPK 432

The following BLAST results are available for this feature:
BLAST of Cp4.1LG15g08580 vs. Swiss-Prot
Match NameE-valueIdentityDescription
T5H_TAXCU1.3e-9440.86Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata PE=1 SV=2[more]
T13H_TAXCU5.4e-8838.29Taxane 13-alpha-hydroxylase OS=Taxus cuspidata GN=CYP725A2 PE=1 SV=1[more]
C16B2_PICSI9.2e-8840.27Cytochrome P450 716B2 OS=Picea sitchensis GN=CYP716B2 PE=2 SV=1[more]
C16B1_PICSI7.8e-8739.59Cytochrome P450 716B1 OS=Picea sitchensis GN=CYP716B1 PE=2 SV=1[more]
T14H_TAXCU8.6e-8638.96Taxoid 14-beta-hydroxylase OS=Taxus cuspidata PE=1 SV=1[more]
BLAST of Cp4.1LG15g08580 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KEB2_CUCSA2.2e-22185.81Uncharacterized protein OS=Cucumis sativus GN=Csa_6G423370 PE=3 SV=1[more]
V4TFN7_9ROSI2.7e-18773.96Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031591mg PE=3 SV=1[more]
M5WZ42_PRUPE2.3e-18674.08Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017753mg PE=3 SV=1[more]
A0A067FBT4_CITSI8.7e-18673.27Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g035672mg PE=3 SV=1[more]
B9GT44_POPTR2.1e-18473.56Cytochrome P450 family protein OS=Populus trichocarpa GN=POPTR_0002s06150g PE=3 ... [more]
BLAST of Cp4.1LG15g08580 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G42850.13.7e-17266.59 cytochrome P450, family 718[more]
AT5G36110.14.0e-8137.02 cytochrome P450, family 716, subfamily A, polypeptide 1[more]
AT2G29090.14.4e-6432.88 cytochrome P450, family 707, subfamily A, polypeptide 2[more]
AT5G45340.11.9e-6231.35 cytochrome P450, family 707, subfamily A, polypeptide 3[more]
AT3G50660.12.3e-5730.13 Cytochrome P450 superfamily protein[more]
BLAST of Cp4.1LG15g08580 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659097243|ref|XP_008449518.1|6.3e-22287.19PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis melo][more]
gi|449444975|ref|XP_004140249.1|3.1e-22185.81PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis sativus][more]
gi|700192791|gb|KGN47995.1|3.1e-22185.81hypothetical protein Csa_6G423370 [Cucumis sativus][more]
gi|645246652|ref|XP_008229454.1|3.9e-18773.85PREDICTED: taxadiene 5-alpha hydroxylase [Prunus mume][more]
gi|567889346|ref|XP_006437195.1|3.9e-18773.96hypothetical protein CICLE_v10031591mg [Citrus clementina][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0010295 (+)-abscisic acid 8'-hydroxylase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG15g08580.1Cp4.1LG15g08580.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 374..383
score: 1.3E-7coord: 303..314
score: 1.3E-7coord: 383..394
score: 1.3E-7coord: 247..264
score: 1.
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 2..436
score: 7.7
IPR001128Cytochrome P450PFAMPF00067p450coord: 27..418
score: 1.9
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 2..433
score: 1.44
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 342..366
score: 1.2E-15coord: 373..383
score: 1.2E-15coord: 236..253
score: 1.2E-15coord: 383..406
score: 1.2E-15coord: 30..49
score: 1.2
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 376..385
scor
NoneNo IPR availablePANTHERPTHR24286FAMILY NOT NAMEDcoord: 1..440
score: 2.8E
NoneNo IPR availablePANTHERPTHR24286:SF43SUBFAMILY NOT NAMEDcoord: 1..440
score: 2.8E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG15g08580Cucsa.185370Cucumber (Gy14) v1cgycpeB0504
Cp4.1LG15g08580CmaCh19G010660Cucurbita maxima (Rimu)cmacpeB522
Cp4.1LG15g08580Cla005532Watermelon (97103) v1cpewmB234
Cp4.1LG15g08580Csa6G423370Cucumber (Chinese Long) v2cpecuB247
Cp4.1LG15g08580MELO3C014463Melon (DHL92) v3.5.1cpemeB236
Cp4.1LG15g08580ClCG09G022360Watermelon (Charleston Gray)cpewcgB215
Cp4.1LG15g08580CSPI06G22890Wild cucumber (PI 183967)cpecpiB249
Cp4.1LG15g08580Lsi11G016440Bottle gourd (USVL1VR-Ls)cpelsiB201
Cp4.1LG15g08580MELO3C014463.2Melon (DHL92) v3.6.1cpemedB277
Cp4.1LG15g08580CsaV3_6G038010Cucumber (Chinese Long) v3cpecucB0312
Cp4.1LG15g08580Bhi05G001776Wax gourdcpewgoB0328
Cp4.1LG15g08580CsGy6G022100Cucumber (Gy14) v2cgybcpeB748
Cp4.1LG15g08580Carg15690Silver-seed gourdcarcpeB0751
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG15g08580Cucumber (Chinese Long) v3cpecucB0314
Cp4.1LG15g08580Wax gourdcpewgoB0337
Cp4.1LG15g08580Cucurbita pepo (Zucchini)cpecpeB256
Cp4.1LG15g08580Cucurbita pepo (Zucchini)cpecpeB267
Cp4.1LG15g08580Cucurbita pepo (Zucchini)cpecpeB277
Cp4.1LG15g08580Cucurbita maxima (Rimu)cmacpeB115
Cp4.1LG15g08580Cucurbita maxima (Rimu)cmacpeB558
Cp4.1LG15g08580Cucurbita moschata (Rifu)cmocpeB093
Cp4.1LG15g08580Cucurbita moschata (Rifu)cmocpeB481
Cp4.1LG15g08580Cucurbita moschata (Rifu)cmocpeB512
Cp4.1LG15g08580Cucurbita moschata (Rifu)cmocpeB567
Cp4.1LG15g08580Bottle gourd (USVL1VR-Ls)cpelsiB198
Cp4.1LG15g08580Watermelon (Charleston Gray)cpewcgB230
Cp4.1LG15g08580Watermelon (97103) v1cpewmB265
Cp4.1LG15g08580Melon (DHL92) v3.5.1cpemeB211
Cp4.1LG15g08580Melon (DHL92) v3.6.1cpemedB252
Cp4.1LG15g08580Silver-seed gourdcarcpeB0042
Cp4.1LG15g08580Silver-seed gourdcarcpeB0464
Cp4.1LG15g08580Silver-seed gourdcarcpeB0541