Cp4.1LG15g02830 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG15g02830
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionRNA 2'-phosphotransferase, Tpt1 / KptA family
LocationCp4.1LG15 : 3127711 .. 3132681 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCATAGCATGTGATTGCTAATTCCACCGTCGTCTTCAACCTTCCCTCCCTTACCGATGTGGGCTTCTGTTGTTACCATCTGCGGCATCAGATTTATGCGCTGCCGTCCTCTTTTGCTTCCAAGAATCTCTCCTTCTCTATTTTCCATGGATAGGAGCAATACCTCCTCCTTTTCTTCTTTTGCTCATTCCAGTAAAAGGTAATTATGCTCCTTCCTTCATCATCATCTTTGAAACTGGCGGAATATCGAAGGAAATCTAAATCGTTTTCTCTGCGCCGTTTTTCATATTTTAGAATCTTGAGTTCGTATGGTTGTTTTTTGAAATCGTAGAGATGGTTATTGATTCTGTTCTTTGTTTTGATTTTTTCAATTAGCGGTGGAAGAGGAAGAGGTTTGGACATGAGGAATGACAGAGAGAGCACGAGAGGGCGCGGTGGCTCTGGTAAAGACAAAATTGATGCTCTTGGTAGACTCTTGTAAGCATATTTCGTCCATTGGTATCTTGTTTAGATGTTAATTGTCTCGGATTAGTTGTCAAACTCATTGTTTATTAGATTAAGCTTATGAAAGACTGTGTGCATGCTCTTTTATTTTGAGTTATGTTATTTATGGCGAAACATAGGTAATATGTACGTTTGGTGATTCATGAAGCTGAAGCTTAATTCCCTAGAATGGAGATACACAGGATAGGAAGATCAAGCTTTAAATGGAAACCTTAAATCAAATGTTCTGATCAGTTATGCACGTTGAACTTGAGAAAATTCCAAGGGGAGTAGAATACAGTCTCATGGTCTTAATGTTATATTCATTTCTAGTAGTGCTGTTACTTGACACCATATCTTTATAAATTTGGGTGGTTTTTTTTTAACAATTTTGAGTTAAGTTGTTGTGAGGGTTCCTATATCTTACTGTTTATGATTGGCTGGTGCAAAATGAATAAGCCCGAGTAAATGATTGAGAGGAATTCAAAAGTTATGCATAATGCATATACGATTTGTTCTCTATAGCAGTGTGATCTGGTTTATATGTTTATCGATTGATATTTGCCCCTTTTATCTTCAGAACACGCATCTTGCGACATATGGCTTCTGAGTTAAACTTGAATATGAGGAGTGATGGATATGTCAAACTTCAGGATTTGCTAAAGTTGAATTTGAAAACATTTGCCAATATCCCATTGAGATCGCACACGATTGATGACATAAGGGAGGTTGGTCGGAGAATTTTCTTCTTTAGTTGCTTTCGCATGACTTTAATATGTAGATTCAAACTGATATGTAACTTAGGCTTTCCATTGCNCTTTCGTATGACTTCAATATGTAGATTCAAACTGATGTGTAACTTATGCTTTCCATTGCTCAACAATGCTGTTAAAGGCTGTTTGTTCTATTTATCTTGATCCGTTTATCCTTGCTTTTTCTCTCAACTAGTTCCACTATTGTATTTCCTCTTCCACGACTTGTAAATTTCAGTTCAGTTTTGTTTCTGTAAGCATCTAGTATTGATGAGTAGTTTTTTTTTCCCTTCAATGCCAGGCTGTTCGGAAGGATAATAAGCAGCGTTTTAGCCTCCTGGAAGACAATGGAGAGCTCTTCATTCGAGCAAACCAAGGCCATACAGTCACGGTTATTTTCTTTCTTTATAAACCATCAAATCCATTACTGTTACAAAGTCTTGGGGGATTGGCAGTCAAGATCATTCATTGCAAAATTGTTGATTGCTTCACATTAGGTCAAATGCATTTGATTCTTCTTTACATTGACTTATTAGTAATATACGAAATGAAAAATCCGTTGCCGCTTGTTTTCTGTTGTTACTCTATTTTGTGACAATCGATCAGCCTTGTCAGATGAGTGTAGTGTTGTATAAAACTTCCTCAACTCTTGTACGATTGTGATACTGGAATCAGATTGACTATTTTGTGTACACCATCATAAATGGAAAAAACGTCCTTTGCAGGTGGTTGAAACTGAAAGTTTATTAAAACCGATTCTTTCGGCTGAGGAGGTTCCAGGTACTGCGGTTTGATGCCTCAACTTTCCTACTTTTTTCACATATCATCCTTTCCTCCTTGATGTGCATGATGGGAGTATCCATGCATTTTAAACTTCCAATTTTGTTATTCAATTAATCACTTTTTTTCTCCCTCTCTTTATGACAGTTTGCGTACATGGTACTTATAAAACGAATTTGGAATCAATTCTGGCATCTGGTTTGAAACGCATGAAAAGATTGCATGTCCACTTCTCATGTGGTTTGCCAACAGATGGAGAAGTTATCAGTGGTAAGTTAAGAAAATCTAATTGTTACGCTTTCTTAAGAATGTTTCCGATAAAACCATGACGTCACTTTGATCAGCACTTGCTACTTGAGTAAATGTACATTTATATTTTGTAGGGAATAGAAACTGGTTTCTTTGATTGATGAAAATGATTACAAAAATTCCTACCATACATAATTACAAACACACCTCCAATGTGTCTCGAGGGACGATACACTATAGTTACAAATGACGAGATCGTTTTCCATGTTTAAGCAAGAAAAGATTATATGCGAAGTTTAGGTGCTTTGTTGCTTATCTGTGAAGATCTGTGCATTTCTTTCCTGCCATAAGTTCCAATAGAAAGCCCAAATTAATCTCTTTCATAGGATCTTTGTCTCTTTCTTGAATGGATGGCTGATGAGGGACGCATCAAGGAGGGTGAAAGTATCTCCTGGTCTTGTAAAACTCCAACCAAAGGCATCTTGTAAGAAGTTCCAGAATGTTGAATTGTAGGTAGACTGGCTAGATAAATGAATTCGTCTCATGATTGTTCATACATTAGTGGCAGCAACTTGGAGAAATTGTAATCCATGGAGATTTTCAAAGTAGTCTGTCAAAAGTGTTAATGCAAGCTTGACTTAGCTCTCAAATAAGGAACTTGACATCCTTGAGAGATTGCCCCCTCCGTAATAGTTTGTAGAATTTGAATTTTACCATATTGCCGGTAGATGCAAGCTGTTTGGTCAAATAACCTGTGGTAAAAGTCCCACTTTTCTGAAAAGTTCACACCCACCAGTTCCCTTTCTCCGACTGAGTAACTCTGCAAAAGGTGGGATAAAGATGCCCATTCATCCACTTCCATGCAGTATATATTTCACCTCAAGAGTAAATTCCATGATTCAGTATTCCAATTCCACAACTCTGTAATTGATAGGGAGAGATGGTAAAGAATAGGATATTCTTCCAATTGCATATCTTCACCCTCTTTCATTTTCGTTCCATTTCTGGTTTATTTCGTCATTTTCATAAAAATTGAGCATACTGTTCATCATGCTTATTATCAATAAAATTAGCTCATTTCGGTTCTAAACTTTCATATCATGCTTCCTATAACATGCTCCAATACGTACTACAAGCTCACCTTAATTTGAAGTTCCTAGGTGCTACTTATTTAGTTCGGTTGTTACTTTGATGGTCGGTTCGGGTCCTTAATGAGTTTCAAATTTCTTCTAACTTTTTAAATAGGGTTCTAAATTAAGAGAAAATATTAATACCATTAAAATTGGATCGGAAATTGGACAAAAACCGGCCAAATTGGGTGAAAATGTCAATGTTTTGTACATAAATAGTGAAGTATCATTCCTTACTCCTAACCTCCAGCCATGAACAGTATTTTGTATTTTGAACCCCCCAGTTTCCTCAACTCTGAACAAGGCCTTACACACCTCAATCCTTTACACTTTGGACAAGAATCATGCAATTTCATTAAGAATAGAAGAAACTATTGATTTTCGAAGTCTCGAGAAAACTCAAGAACGAACCTTCAATCTCTCAACTTGATCTCTCCCTACAAGTACGTTCAAGTCTCAGTTCCTTCACAAACTGTGTAAAGCTTCCTCACACAAAACTATACCCCCTGAAATCATGGAAATCCATTAACATTCGAGGTAAAATCACTTGAAGAAGATTTGGTTCATAGCCACGAACACTTACCTCAAATTCGAATTCGAATCCCTCCTTCTCATGCCTCTTTGCTCCAGTTCTTTCCTCAATCCCTTACCGTAGGATCCTCCAAGTTTCAAGTCCAGAGCTGGCCCCAATCATCACACATCTCTCCTCAAAGATCGTTGGCTGTTCTTCTGCCTTCTCTTTTATTTATTTATTTGTTATTTGTCATTTGTTTATTATTTATTTTGGTTCACTCTAAGGCTAACTTGTTTCCTGTTTCTGGAAATGGACAGGTATGAGACGCAACGTTAATGTTTTAATCTTTCTTGATGTCAATAAAGCTCTGAATGGTACTGCTGAACCCTCTTTTATTTTCATTTGTATAATACTGATGATCTCTAATGCCTGTTTCTTGCTAAAACATTGTATTTCAGATGGAATGAAGCTTTACATGTCGGACAACAAGGTCATTCTGACCGAAGGTTTCGACGGAGTGGTGGCAGTCAAATACTTTCAAAAAATCGAGTCATGGCCAAATCGACAACCAATTCCATTTTGAGCCTGGATGAGATGGTGTACATGATCAATCATTTCATGAAAATATTGAATTGTGTTTAGATCTAGAGACAAGATATTAACACAATCATAACTGGAAATTTCTTATTGGATTTGCACCAGATTCTCGTATGCTAACTTTTATAGCTCGGGGTTAGCTCGGGTTTCATATGAATTTCAAACTTGGGGAGTCTTTGCATTTAAGTTGGCAGCAAACAGTTCTGTCCTTCTTACGAACATGATGGTGGATCTTTCATGAAGATTTGGCAACAAGTTTAGATTTGTTCATGAAGCGATGAAAGTTAGTATCCAAGTCTGTGGAGAGTGATATGAGAAAGCAGCCTCTGAAGAAGTGTATGATGATATTTCTTCATTTTTGATTCAGTTTGTATTGGTGAGTGAAGAGTATGGTTAACAATTCTGATGTAATGCAACCTGTACCACAGGACTTGCTTTATGAAATTTCTGATAATAATAATAATAA

mRNA sequence

CGCATAGCATGTGATTGCTAATTCCACCGTCGTCTTCAACCTTCCCTCCCTTACCGATGTGGGCTTCTGTTGTTACCATCTGCGGCATCAGATTTATGCGCTGCCGTCCTCTTTTGCTTCCAAGAATCTCTCCTTCTCTATTTTCCATGGATAGGAGCAATACCTCCTCCTTTTCTTCTTTTGCTCATTCCAGTAAAAGCGGTGGAAGAGGAAGAGGTTTGGACATGAGGAATGACAGAGAGAGCACGAGAGGGCGCGGTGGCTCTGGTAAAGACAAAATTGATGCTCTTGGTAGACTCTTAACACGCATCTTGCGACATATGGCTTCTGAGTTAAACTTGAATATGAGGAGTGATGGATATGTCAAACTTCAGGATTTGCTAAAGTTGAATTTGAAAACATTTGCCAATATCCCATTGAGATCGCACACGATTGATGACATAAGGGAGGCTGTTCGGAAGGATAATAAGCAGCGTTTTAGCCTCCTGGAAGACAATGGAGAGCTCTTCATTCGAGCAAACCAAGGCCATACAGTCACGGTGGTTGAAACTGAAAGTTTATTAAAACCGATTCTTTCGGCTGAGGAGGTTCCAGTTTGCGTACATGGTACTTATAAAACGAATTTGGAATCAATTCTGGCATCTGGTTTGAAACGCATGAAAAGATTGCATGTCCACTTCTCATGTGGTTTGCCAACAGATGGAGAAGTTATCAGTGGTATGAGACGCAACGTTAATGTTTTAATCTTTCTTGATGTCAATAAAGCTCTGAATGATGGAATGAAGCTTTACATGTCGGACAACAAGGTCATTCTGACCGAAGGTTTCGACGGAGTGGTGGCAGTCAAATACTTTCAAAAAATCGAGTCATGGCCAAATCGACAACCAATTCCATTTTGAGCCTGGATGAGATGATTCTCGTATGCTAACTTTTATAGCTCGGGGTTAGCTCGGGTTTCATATGAATTTCAAACTTGGGGAGTCTTTGCATTTAAGTTGGCAGCAAACAGTTCTGTCCTTCTTACGAACATGATGGTGGATCTTTCATGAAGATTTGGCAACAAGTTTAGATTTGTTCATGAAGCGATGAAAGTTAGTATCCAAGTCTGTGGAGAGTGATATGAGAAAGCAGCCTCTGAAGAAGTGTATGATGATATTTCTTCATTTTTGATTCAGTTTGTATTGGTGAGTGAAGAGTATGGTTAACAATTCTGATGTAATGCAACCTGTACCACAGGACTTGCTTTATGAAATTTCTGATAATAATAATAATAA

Coding sequence (CDS)

ATGTGGGCTTCTGTTGTTACCATCTGCGGCATCAGATTTATGCGCTGCCGTCCTCTTTTGCTTCCAAGAATCTCTCCTTCTCTATTTTCCATGGATAGGAGCAATACCTCCTCCTTTTCTTCTTTTGCTCATTCCAGTAAAAGCGGTGGAAGAGGAAGAGGTTTGGACATGAGGAATGACAGAGAGAGCACGAGAGGGCGCGGTGGCTCTGGTAAAGACAAAATTGATGCTCTTGGTAGACTCTTAACACGCATCTTGCGACATATGGCTTCTGAGTTAAACTTGAATATGAGGAGTGATGGATATGTCAAACTTCAGGATTTGCTAAAGTTGAATTTGAAAACATTTGCCAATATCCCATTGAGATCGCACACGATTGATGACATAAGGGAGGCTGTTCGGAAGGATAATAAGCAGCGTTTTAGCCTCCTGGAAGACAATGGAGAGCTCTTCATTCGAGCAAACCAAGGCCATACAGTCACGGTGGTTGAAACTGAAAGTTTATTAAAACCGATTCTTTCGGCTGAGGAGGTTCCAGTTTGCGTACATGGTACTTATAAAACGAATTTGGAATCAATTCTGGCATCTGGTTTGAAACGCATGAAAAGATTGCATGTCCACTTCTCATGTGGTTTGCCAACAGATGGAGAAGTTATCAGTGGTATGAGACGCAACGTTAATGTTTTAATCTTTCTTGATGTCAATAAAGCTCTGAATGATGGAATGAAGCTTTACATGTCGGACAACAAGGTCATTCTGACCGAAGGTTTCGACGGAGTGGTGGCAGTCAAATACTTTCAAAAAATCGAGTCATGGCCAAATCGACAACCAATTCCATTTTGA

Protein sequence

MWASVVTICGIRFMRCRPLLLPRISPSLFSMDRSNTSSFSSFAHSSKSGGRGRGLDMRNDRESTRGRGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKLQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGHTVTVVETESLLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNVNVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPIPF
Homology
[Close]
BLAST of Cp4.1LG15g02830 vs. Swiss-Prot
Match: TRPT1_DANRE (tRNA 2'-phosphotransferase 1 OS=Danio rerio GN=trpt1 PE=2 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 4.8e-34
Identity = 87/230 (37.83%), Postives = 138/230 (60.00%), Query Frame = 1 

Query: 55  LDMRNDRESTRGRGGSGKDKIDA-LGRLLTRILRHMASELNLNMRSDGYVKLQDLLKLNL 114
           +D        RGRG   ++  D  L + L+ +LRH AS++ L M SDG+V +++LL    
Sbjct: 1   MDCETRGRGRRGRGNRNEESRDVRLSKSLSYVLRHGASKMGLQMNSDGFVFVEELL---- 60

Query: 115 KTFANIPLRSHTIDDIREAVRKDNKQRFSLLE--DNGELFIRANQGHTVTVVETESLLKP 174
              A+   RS ++DD+   V  ++KQRF L +  ++  L IRANQGH+V V + E L + 
Sbjct: 61  ---AHQQFRSFSVDDVERVVASNDKQRFKLCKHPEDDRLQIRANQGHSVQVTDLE-LREI 120

Query: 175 ILSAEEVP-VCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNVNVLI 234
               ++ P   VHG+Y  +  SI + GL RM R H+H + GLP +G VISGMR++ ++ +
Sbjct: 121 SQDDQDYPREAVHGSYMKHWPSIRSQGLSRMNRTHIHLAPGLPGEGRVISGMRQSCDLAV 180

Query: 235 FLDVNKALNDGMKLYMSDNKVILTEG-FDGVVAVKYFQKIESWPNRQPIP 280
           ++DV KA++DG+K + S+N V+LT G   G++A  YF + +     +P+P
Sbjct: 181 YIDVTKAMSDGIKFFWSENGVLLTPGDAAGILAPCYFSRAQ---RLKPLP 219

[Close]
BLAST of Cp4.1LG15g02830 vs. Swiss-Prot
Match: TPT1_SCHPO (Putative tRNA 2'-phosphotransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC2C4.12c PE=3 SV=2)

HSP 1 Score: 139.8 bits (351), Expect = 4.5e-32
Identity = 74/192 (38.54%), Postives = 118/192 (61.46%), Query Frame = 1 

Query: 80  RLLTRILRHMASELNLNMRSDGYVKLQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQ 139
           + L+++LRH A    L +R DGY+++  +LKL          R   ++ +   V+ ++K+
Sbjct: 43  KALSKVLRHTAKANGLQIREDGYIEVDSILKLP-------QFRGMGMELLLSIVKGNDKK 102

Query: 140 RFSLLEDNGELFIRANQGHTVTVVETESLLKPILSAEEVPVCVHGTYKTNLESILASGLK 199
           RF++ E  G L+IRANQGH++  V+    +  I +A  +P  VHGT K     I   GL 
Sbjct: 103 RFTMEEVEGVLYIRANQGHSIKAVQVP--MARIDNASSIPKVVHGTKKELWPVISKQGLS 162

Query: 200 RMKRLHVHFSCGLPTDGEVISGMRRNVNVLIFLDVNKALNDGMKLYMSDNKVILTEGFDG 259
           RMKR H+H + GL  D  VISG+R++  + I++D  KA+ DG++ Y S+N VILTEG +G
Sbjct: 163 RMKRNHIHCATGLYGDPGVISGIRKSCTLYIYIDSAKAMQDGVEFYRSENGVILTEGVNG 222

Query: 260 VVAVKYFQKIES 272
           +++ KYF ++E+
Sbjct: 223 LLSSKYFSRVET 225

[Close]
BLAST of Cp4.1LG15g02830 vs. Swiss-Prot
Match: TRPT1_MOUSE (tRNA 2'-phosphotransferase 1 OS=Mus musculus GN=Trpt1 PE=2 SV=2)

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-30
Identity = 89/223 (39.91%), Postives = 129/223 (57.85%), Query Frame = 1 

Query: 61  RESTRGRGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKLQDLLKLNLKTFANIP 120
           +E  R      +D+   L + L+  LRH A +L L MR+DG+V LQ LL+L         
Sbjct: 9   KEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLP-------Q 68

Query: 121 LRSHTIDDIREAVRKDNKQRFSLL--EDNGELFIRANQGHTVTVVETESLLKPILSAEEV 180
             S +I+D++  V  + KQRF+L   E +  L IRANQGH++ V E E  L P+ + + +
Sbjct: 69  FHSFSIEDVQLVVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELE--LTPLETPQAL 128

Query: 181 PVC-VHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNVNVLIFLDVNKA 240
           P+  VHGT+  +  SIL  GL R  R H+H + GLP D  VISG+R N  V +F+D   A
Sbjct: 129 PLTLVHGTFWKHWPSILLKGLSRQGRTHIHLASGLPGDPGVISGIRPNCEVAVFIDGPLA 188

Query: 241 LNDGMKLYMSDNKVILTEG-FDGVVAVKYFQK-IESWPNRQPI 279
           L DG+  + S N VILT G  +G +  KYF++ ++  P R+P+
Sbjct: 189 LTDGIPFFCSANGVILTPGNAEGFLLPKYFKEALQLRPTRKPL 222

[Close]
BLAST of Cp4.1LG15g02830 vs. Swiss-Prot
Match: TRPT1_BOVIN (tRNA 2'-phosphotransferase 1 OS=Bos taurus GN=TRPT1 PE=2 SV=3)

HSP 1 Score: 125.2 bits (313), Expect = 1.1e-27
Identity = 87/232 (37.50%), Postives = 129/232 (55.60%), Query Frame = 1 

Query: 52  GRGLDMRNDRESTRGRGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKLQDLLKL 111
           GR  +    +     R    +D+   L + L+  LRH A +L L M +DG+V L  LL+L
Sbjct: 6   GRRRETAGPKGRRAHRPPQDQDRDVQLSKALSYALRHGALKLGLPMGADGFVPLDALLQL 65

Query: 112 NLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGEL--FIRANQGHTVTVVETESLL 171
                     RS + +D++  V  + KQRF+L   +      IRANQGH++ V E E  L
Sbjct: 66  P-------QFRSFSAEDVQRVVDTNVKQRFALQPGDPSTGPLIRANQGHSLQVPELE--L 125

Query: 172 KPILSAEEVPVC-VHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNVNV 231
           +P+ + + +P+  VHGT++ +  SIL  GL    R H+H + GLP D  VISGMR N  V
Sbjct: 126 EPLETPQALPLMLVHGTFRQHWPSILLKGLSCRGRTHIHLAPGLPGDPGVISGMRPNCEV 185

Query: 232 LIFLDVNKALNDGMKLYMSDNKVILTEG-FDGVVAVKYFQK-IESWPNRQPI 279
            +F++   AL DG+  + S N VILT G  DGV+  KYF++ ++  P R+P+
Sbjct: 186 AVFINGPLALADGIPFFRSTNGVILTPGNADGVLPPKYFKEALQLRPTRKPL 228

[Close]
BLAST of Cp4.1LG15g02830 vs. Swiss-Prot
Match: TPT1_YEAST (tRNA 2'-phosphotransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TPT1 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.4e-19
Identity = 74/205 (36.10%), Postives = 114/205 (55.61%), Query Frame = 1 

Query: 72  KDKIDA-LGRLLTRILRHMASELNLNMRSDGYVKLQDLLKLN-LKTFANIPLRSHTIDDI 131
           KDK D  L + L+ +LRH A +  L + S+GY  L++LL  N LKT         T+DDI
Sbjct: 7   KDKRDVQLSKALSYLLRHTAVKEKLTIDSNGYTPLKELLSHNRLKT------HKCTVDDI 66

Query: 132 REAVRKDNKQRFSLLE-DNGELFIRANQGHTVTVVE-TESLLKPILSAEEVP-VCVHGTY 191
              V++++KQRF +      E +I A QGH++  ++ ++ +L PI  A ++P   +HGT 
Sbjct: 67  HRIVKENDKQRFHIKTLGADEEWICATQGHSIKSIQPSDEVLVPITEASQLPQELIHGTN 126

Query: 192 KTNLESILASG-LKRMKRLHVHFSCGLPTDGEVISGMRRNVNVLIFLDVNKAL-NDGMKL 251
             ++  I+ SG +  M R HVH S G+     VISGMR + NV IF+D +  L    +K+
Sbjct: 127 LQSVIKIIESGAISPMSRNHVHLSPGMLHAKGVISGMRSSSNVYIFIDCHSPLFFQTLKM 186

Query: 252 YMSDNKVILTEGFDGVVAVKYFQKI 270
           + S N V L+      + V+  QK+
Sbjct: 187 FRSLNNVYLSSS----IPVELIQKV 201

[Close]
BLAST of Cp4.1LG15g02830 vs. TrEMBL
Match: A0A0A0L3T7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G126200 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 3.4e-135
Identity = 251/293 (85.67%), Postives = 265/293 (90.44%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPR---------ISPSLFSMDRSNTSSFSSFAHSSKSGGR 60
           MWASVVT CGI+ +RCRPL LPR         ISPSL SMDRSNT+S SSFAHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRPLWLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMRNDRESTRGRGG----SGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKLQD 120
           GR LD+RNDRE TRGRGG    SGKDKIDALGRLLTR+LRHMASELNLNMRSDGYVK++D
Sbjct: 61  GRDLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRVLRHMASELNLNMRSDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFS L++NGEL IRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSFLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNVN 240
           LLKPILSAEEVPVCVHGTYK NLESIL SGLKRMKRLHVHFSCGLPTDGEVISGMRR+VN
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISGMRRDVN 240

Query: 241 VLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPIPF 281
           VLIFLDV KAL DGMKLY+SDN+VILTEGFDGVV +KYFQKIESWPNRQP+PF
Sbjct: 241 VLIFLDVQKALEDGMKLYISDNRVILTEGFDGVVPMKYFQKIESWPNRQPVPF 293

[Close]
BLAST of Cp4.1LG15g02830 vs. TrEMBL
Match: D7U7X7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0048g00300 PE=4 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.4e-117
Identity = 230/302 (76.16%), Postives = 255/302 (84.44%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPRISPS---------------LFSMDRSN--TSSFSSFA 60
           MWASV T  GIR +R   + LP ++P+               L SM+ ++  +SSFS+FA
Sbjct: 1   MWASV-TGNGIRILRYCYVCLPPVTPTPSSLSGLSLLTRFGVLVSMENNSAKSSSFSAFA 60

Query: 61  HSSKSGGRGRGLDMRNDRESTRGRGG-----SGKDKIDALGRLLTRILRHMASELNLNMR 120
           HSS+SGGRGRG  MR+DRE +RGRGG     SGKDKIDALGRLLTRILRHMASELNLNMR
Sbjct: 61  HSSRSGGRGRGGTMRDDRERSRGRGGGGGGSSGKDKIDALGRLLTRILRHMASELNLNMR 120

Query: 121 SDGYVKLQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGH 180
           SDGYV++QDLLKLN+KTFANIPLRSHTIDDIREAVRKDNKQRF LLE+NGEL IRANQGH
Sbjct: 121 SDGYVRVQDLLKLNMKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGH 180

Query: 181 TVTVVETESLLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEV 240
           TVT+VE+ESLLKPILSAEEV VCVHG+YK NLESIL SGLK MKRLHVHFSCGLP DGEV
Sbjct: 181 TVTIVESESLLKPILSAEEVTVCVHGSYKRNLESILESGLKHMKRLHVHFSCGLPMDGEV 240

Query: 241 ISGMRRNVNVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPI 281
           ISGMRR+VN+LIFLDV KAL DGMKLY+SDNKVILTEGFDGVV VKYF+KIESWP+RQ I
Sbjct: 241 ISGMRRDVNLLIFLDVRKALEDGMKLYISDNKVILTEGFDGVVPVKYFEKIESWPDRQTI 300

[Close]
BLAST of Cp4.1LG15g02830 vs. TrEMBL
Match: A0A0B2PYM4_GLYSO (tRNA 2'-phosphotransferase 1 OS=Glycine soja GN=glysoja_008635 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 5.4e-117
Identity = 226/294 (76.87%), Postives = 247/294 (84.01%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPRISPSLFSM-----------DRSNTSSFSSFAHSSKSG 60
           M+ASV T      +R   LL PR++P   S+           + + ++ FSS+AH+S S 
Sbjct: 1   MFASV-TNSSTNILRHCALLRPRVTPIFLSLLHRLGASAPMDNNARSNPFSSYAHASPST 60

Query: 61  GRGRGLDMRNDRESTRGRGG---SGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKLQ 120
           GRG+  DMRNDRE TRGRGG   SGKDKID+LGRLLTRILRHMASELNLNMRSDGYVK+ 
Sbjct: 61  GRGKAFDMRNDRERTRGRGGGSGSGKDKIDSLGRLLTRILRHMASELNLNMRSDGYVKVN 120

Query: 121 DLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGHTVTVVETE 180
           DLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLE+NGEL IRANQGHTV  VETE
Sbjct: 121 DLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVKAVETE 180

Query: 181 SLLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNV 240
           SLLKPILSAEE PVCVHGTY+ NLESIL SGLKRMKRLHVHFSCGLPTDGEVISGMR++V
Sbjct: 181 SLLKPILSAEEFPVCVHGTYRKNLESILGSGLKRMKRLHVHFSCGLPTDGEVISGMRQDV 240

Query: 241 NVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPIPF 281
           NVLIFLDV KAL +GMKLY+SDNKVILTEGFDGVVA KYF+KIESWP RQPIPF
Sbjct: 241 NVLIFLDVRKALEEGMKLYISDNKVILTEGFDGVVAPKYFEKIESWPGRQPIPF 293

[Close]
BLAST of Cp4.1LG15g02830 vs. TrEMBL
Match: A0A151TBS2_CAJCA (tRNA 2'-phosphotransferase 1 OS=Cajanus cajan GN=KK1_019074 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.1e-116
Identity = 230/297 (77.44%), Postives = 245/297 (82.49%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPRIS---PSLF---------SMDRSNTSSFSSFAHSSKS 60
           M+ASV     I   R   L LPR++   PSL          +  R+NT+ FSS AH+S S
Sbjct: 1   MFASVTNSSSIIISRTYALFLPRVTVTFPSLLHRFRASAPMANPRNNTNPFSSHAHASPS 60

Query: 61  GGRGRG--LDMRNDRESTRGRGG---SGKDKIDALGRLLTRILRHMASELNLNMRSDGYV 120
            G GRG   DMRNDRE TRGRGG   SGKDKIDALGRLLTRILRHMASELNLNMRSDG+V
Sbjct: 61  AGSGRGKAFDMRNDRERTRGRGGGSGSGKDKIDALGRLLTRILRHMASELNLNMRSDGFV 120

Query: 121 KLQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGHTVTVV 180
           K+ DLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSL+EDNGEL IRANQGHTVT V
Sbjct: 121 KVNDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLIEDNGELLIRANQGHTVTAV 180

Query: 181 ETESLLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMR 240
           ETESLLKPIL AEEVPVCVHGTY+ NLESIL SGLKRMKRLHVHFSCGLPTDGEVISGMR
Sbjct: 181 ETESLLKPILLAEEVPVCVHGTYRKNLESILGSGLKRMKRLHVHFSCGLPTDGEVISGMR 240

Query: 241 RNVNVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPIPF 281
           R+VNVLIFLDV KAL +GMKLY+SDNKVILTEGFDG V  KYF+KIESWP RQPI F
Sbjct: 241 RDVNVLIFLDVRKALEEGMKLYISDNKVILTEGFDGAVPPKYFEKIESWPGRQPIHF 297

[Close]
BLAST of Cp4.1LG15g02830 vs. TrEMBL
Match: K7MNY6_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_17G254400 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.0e-115
Identity = 223/294 (75.85%), Postives = 246/294 (83.67%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPRISPSLFSM-----------DRSNTSSFSSFAHSSKSG 60
           M+ASV T      +R   LL PR++P   S+           + + ++ FSS+AH+S S 
Sbjct: 1   MFASV-TNSSTNILRHCALLRPRVTPIFLSLLHRLGASAPMDNNARSNPFSSYAHASPST 60

Query: 61  GRGRGLDMRNDRESTRGRGG---SGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKLQ 120
           GRG+  DMRNDRE TRGRGG   SGKDKID+LGRLLTRILRHMASELNLNMRSDGYVK+ 
Sbjct: 61  GRGKAFDMRNDRERTRGRGGGSGSGKDKIDSLGRLLTRILRHMASELNLNMRSDGYVKVN 120

Query: 121 DLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGHTVTVVETE 180
           DLLKLNLKTFANIPL+ HTIDDIREAVRKDNKQRFSLLE+NGEL IRANQGHTV  VETE
Sbjct: 121 DLLKLNLKTFANIPLKLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVKAVETE 180

Query: 181 SLLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNV 240
           SLLKPILSAEE PVCVHGTY+ NLESIL SGLKRMKRLHVHFSCGLPTDGEVISGMR++V
Sbjct: 181 SLLKPILSAEEFPVCVHGTYRKNLESILGSGLKRMKRLHVHFSCGLPTDGEVISGMRQDV 240

Query: 241 NVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPIPF 281
           NVLIFLDV KAL +GMKLY+SDNKVILTEGFDGVV  KYF+KIESWP+RQPIPF
Sbjct: 241 NVLIFLDVRKALEEGMKLYISDNKVILTEGFDGVVPAKYFEKIESWPSRQPIPF 293

[Close]
BLAST of Cp4.1LG15g02830 vs. TAIR10
Match: AT2G45330.1 (AT2G45330.1 RNA 2'-phosphotransferase, Tpt1 / KptA family)

HSP 1 Score: 379.4 bits (973), Expect = 1.9e-105
Identity = 189/249 (75.90%), Postives = 215/249 (86.35%), Query Frame = 1 

Query: 34  SNTSSFSSFAHSSKSGGRGRGLDMRNDRESTRGRG--GSGKDKIDALGRLLTRILRHMAS 93
           S  S+ SSFA SS+SGGRG G +  NDR   +GRG  G GKD+IDALGRLLTRILRHMA+
Sbjct: 41  SRKSNVSSFAQSSRSGGRGGGYERDNDRRRPQGRGDGGGGKDRIDALGRLLTRILRHMAT 100

Query: 94  ELNLNMRSDGYVKLQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELF 153
           EL LNMR DG+VK++DLL LNLKT ANI L+SHTID+IREAVR+DNKQRFSL+++NGEL 
Sbjct: 101 ELRLNMRGDGFVKVEDLLNLNLKTSANIQLKSHTIDEIREAVRRDNKQRFSLIDENGELL 160

Query: 154 IRANQGHTVTVVETESLLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCG 213
           IRANQGH++T VE+E LLKPILS EE PVCVHGTY+ NLESILASGLKRM R+HVHFSCG
Sbjct: 161 IRANQGHSITTVESEKLLKPILSPEEAPVCVHGTYRKNLESILASGLKRMNRMHVHFSCG 220

Query: 214 LPTDGEVISGMRRNVNVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIES 273
           LPTDGEVISGMRRNVNV+IFLD+ KAL DG+  Y+SDNKVILTEG DGV+ V YFQKIES
Sbjct: 221 LPTDGEVISGMRRNVNVIIFLDIKKALEDGIAFYISDNKVILTEGIDGVLPVDYFQKIES 280

Query: 274 WPNRQPIPF 281
           WP+RQ IPF
Sbjct: 281 WPDRQSIPF 289

[Close]
BLAST of Cp4.1LG15g02830 vs. TAIR10
Match: AT5G23600.1 (AT5G23600.1 RNA 2'-phosphotransferase, Tpt1 / KptA family)

HSP 1 Score: 331.3 bits (848), Expect = 5.9e-91
Identity = 158/208 (75.96%), Postives = 184/208 (88.46%), Query Frame = 1 

Query: 73  DKIDALGRLLTRILRHMASELNLNMRSDGYVKLQDLLKLNLKTFANIPLRSHTIDDIREA 132
           D+IDALGR+LTRILRHMA+EL LNMR DG+VK++DLL LNLKT ANI L SHTID+IREA
Sbjct: 5   DRIDALGRILTRILRHMATELRLNMRGDGFVKVEDLLNLNLKTCANIQLNSHTIDEIREA 64

Query: 133 VRKDNKQRFSLLEDNGELFIRANQGHTVTVVETESLLKPILSAEEVPVCVHGTYKTNLES 192
           V +DNK+RFSL++++GEL IRANQGH++T VE+E LLKPILS EE PVCVHGTY+ NLES
Sbjct: 65  VTRDNKKRFSLIDEDGELLIRANQGHSITTVESEKLLKPILSPEEAPVCVHGTYRKNLES 124

Query: 193 ILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNVNVLIFLDVNKALNDGMKLYMSDNKVI 252
           ILASGLKRM R+HVHFSCGLPTDGEVISG+RRNVNV+IFL + KAL DG+  Y+SDNKVI
Sbjct: 125 ILASGLKRMNRMHVHFSCGLPTDGEVISGVRRNVNVIIFLHIKKALEDGIAFYISDNKVI 184

Query: 253 LTEGFDGVVAVKYFQKIESWPNRQPIPF 281
           LT+G  GV+ V YFQKIESWP+RQ IPF
Sbjct: 185 LTQGIVGVLPVDYFQKIESWPDRQSIPF 212

[Close]
BLAST of Cp4.1LG15g02830 vs. NCBI nr
Match: gi|778677447|ref|XP_004133917.2| (PREDICTED: tRNA 2'-phosphotransferase 1 [Cucumis sativus])

HSP 1 Score: 489.2 bits (1258), Expect = 4.8e-135
Identity = 251/293 (85.67%), Postives = 265/293 (90.44%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPR---------ISPSLFSMDRSNTSSFSSFAHSSKSGGR 60
           MWASVVT CGI+ +RCRPL LPR         ISPSL SMDRSNT+S SSFAHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRPLWLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMRNDRESTRGRGG----SGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKLQD 120
           GR LD+RNDRE TRGRGG    SGKDKIDALGRLLTR+LRHMASELNLNMRSDGYVK++D
Sbjct: 61  GRDLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRVLRHMASELNLNMRSDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFS L++NGEL IRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSFLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNVN 240
           LLKPILSAEEVPVCVHGTYK NLESIL SGLKRMKRLHVHFSCGLPTDGEVISGMRR+VN
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISGMRRDVN 240

Query: 241 VLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPIPF 281
           VLIFLDV KAL DGMKLY+SDN+VILTEGFDGVV +KYFQKIESWPNRQP+PF
Sbjct: 241 VLIFLDVQKALEDGMKLYISDNRVILTEGFDGVVPMKYFQKIESWPNRQPVPF 293

[Close]
BLAST of Cp4.1LG15g02830 vs. NCBI nr
Match: gi|659075488|ref|XP_008438171.1| (PREDICTED: tRNA 2'-phosphotransferase 1 [Cucumis melo])

HSP 1 Score: 488.8 bits (1257), Expect = 6.3e-135
Identity = 252/293 (86.01%), Postives = 266/293 (90.78%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPR---------ISPSLFSMDRSNTSSFSSFAHSSKSGGR 60
           MWASVVT CGI+ +RCR LLLPR         ISPSL SMDRSNT+S SSFAHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMRNDRESTRGRGG----SGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKLQD 120
           GRGLD+RNDRE TRGRGG    SGKDKIDALGRLLTRILRHMASELNLNMR+DGYVK++D
Sbjct: 61  GRGLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLL++NGEL IRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGMRRNVN 240
           LLKPILSAEEVPVCVHGTYK NLESIL SGLKRMKRLHVHFSCGLPTDGEVISGMRR+VN
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISGMRRDVN 240

Query: 241 VLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPIPF 281
           VLIFLDV KAL DGMKLY+SDN+VILTEGFDG+V +KYFQKIESWPNRQPI F
Sbjct: 241 VLIFLDVQKALEDGMKLYISDNRVILTEGFDGMVPIKYFQKIESWPNRQPISF 293

[Close]
BLAST of Cp4.1LG15g02830 vs. NCBI nr
Match: gi|731421252|ref|XP_010661668.1| (PREDICTED: tRNA 2'-phosphotransferase 1 isoform X3 [Vitis vinifera])

HSP 1 Score: 435.6 bits (1119), Expect = 6.3e-119
Identity = 231/298 (77.52%), Postives = 258/298 (86.58%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPRISPS------------LFSMDRSN--TSSFSSFAHSS 60
           MWASV T  GIRF+R   + LP ++P+            L SM+ ++  +SSFS+FAHSS
Sbjct: 1   MWASV-TGNGIRFLRYCYVCLPPVTPTPSSLSLLTRFGALVSMENNSAKSSSFSAFAHSS 60

Query: 61  KSGGRGRGLDMRNDRESTRGRGG----SGKDKIDALGRLLTRILRHMASELNLNMRSDGY 120
           +SGGRGRG  MR+DRE +RGRGG    SGKDKIDALGRLLTRILRHMASELNLNMRSDGY
Sbjct: 61  RSGGRGRGGTMRDDRERSRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGY 120

Query: 121 VKLQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGHTVTV 180
           ++++DLLKLN+KTFANIPLRSHTIDDIREAVRKDNKQRFSLLE+NGEL IRANQGHTVT+
Sbjct: 121 IRVEDLLKLNMKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTI 180

Query: 181 VETESLLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISGM 240
           VE+ESLLKPILSAEEV VCVHG+YK NLESIL SGLKRMKRLHVHFSCGLP DGEVISGM
Sbjct: 181 VESESLLKPILSAEEVTVCVHGSYKRNLESILESGLKRMKRLHVHFSCGLPMDGEVISGM 240

Query: 241 RRNVNVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPIPF 281
           RR+VN+LIFLDV KAL DGMKLY+SDNKVILTEGFDGVV VKYF+KIESWP+RQ IPF
Sbjct: 241 RRDVNLLIFLDVRKALEDGMKLYISDNKVILTEGFDGVVPVKYFEKIESWPDRQTIPF 297

[Close]
BLAST of Cp4.1LG15g02830 vs. NCBI nr
Match: gi|731421250|ref|XP_010661667.1| (PREDICTED: tRNA 2'-phosphotransferase 1 isoform X2 [Vitis vinifera])

HSP 1 Score: 432.2 bits (1110), Expect = 7.0e-118
Identity = 231/302 (76.49%), Postives = 256/302 (84.77%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPRISPS------------LFSMDRSN--TSSFSSFAHSS 60
           MWASV T  GIRF+R   + LP ++P+            L SM+ ++  +SSFS+FAHSS
Sbjct: 1   MWASV-TGNGIRFLRYCYVCLPPVTPTPSSLSLLTRFGALVSMENNSAKSSSFSAFAHSS 60

Query: 61  KSGGRGRGLDMRNDRESTRGRGG--------SGKDKIDALGRLLTRILRHMASELNLNMR 120
           +SGGRGRG  MR+DRE +RGRGG        SGKDKIDALGRLLTRILRHMASELNLNMR
Sbjct: 61  RSGGRGRGGTMRDDRERSRGRGGGGGGGGGSSGKDKIDALGRLLTRILRHMASELNLNMR 120

Query: 121 SDGYVKLQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGH 180
           SDGYV++QDLLKLN+KTFANIPLRSHTIDDIREAVRKDNKQRF LLE+NGEL IRANQGH
Sbjct: 121 SDGYVRVQDLLKLNMKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGH 180

Query: 181 TVTVVETESLLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEV 240
           TVT+VE+ESLLKPILSAEEV VCVHG+YK NLESIL SGLK MKRLHVHFSCGLP DGEV
Sbjct: 181 TVTIVESESLLKPILSAEEVTVCVHGSYKRNLESILESGLKHMKRLHVHFSCGLPMDGEV 240

Query: 241 ISGMRRNVNVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPI 281
           ISGMRR+VN+LIFLDV KAL DGMKLY+SDNKVILTEGFDGVV VKYF+KIESWP+RQ I
Sbjct: 241 ISGMRRDVNLLIFLDVRKALEDGMKLYISDNKVILTEGFDGVVPVKYFEKIESWPDRQTI 300

[Close]
BLAST of Cp4.1LG15g02830 vs. NCBI nr
Match: gi|225454024|ref|XP_002274805.1| (PREDICTED: tRNA 2'-phosphotransferase 1 isoform X1 [Vitis vinifera])

HSP 1 Score: 429.9 bits (1104), Expect = 3.5e-117
Identity = 230/302 (76.16%), Postives = 255/302 (84.44%), Query Frame = 1 

Query: 1   MWASVVTICGIRFMRCRPLLLPRISPS---------------LFSMDRSN--TSSFSSFA 60
           MWASV T  GIR +R   + LP ++P+               L SM+ ++  +SSFS+FA
Sbjct: 1   MWASV-TGNGIRILRYCYVCLPPVTPTPSSLSGLSLLTRFGVLVSMENNSAKSSSFSAFA 60

Query: 61  HSSKSGGRGRGLDMRNDRESTRGRGG-----SGKDKIDALGRLLTRILRHMASELNLNMR 120
           HSS+SGGRGRG  MR+DRE +RGRGG     SGKDKIDALGRLLTRILRHMASELNLNMR
Sbjct: 61  HSSRSGGRGRGGTMRDDRERSRGRGGGGGGSSGKDKIDALGRLLTRILRHMASELNLNMR 120

Query: 121 SDGYVKLQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLEDNGELFIRANQGH 180
           SDGYV++QDLLKLN+KTFANIPLRSHTIDDIREAVRKDNKQRF LLE+NGEL IRANQGH
Sbjct: 121 SDGYVRVQDLLKLNMKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGH 180

Query: 181 TVTVVETESLLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEV 240
           TVT+VE+ESLLKPILSAEEV VCVHG+YK NLESIL SGLK MKRLHVHFSCGLP DGEV
Sbjct: 181 TVTIVESESLLKPILSAEEVTVCVHGSYKRNLESILESGLKHMKRLHVHFSCGLPMDGEV 240

Query: 241 ISGMRRNVNVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAVKYFQKIESWPNRQPI 281
           ISGMRR+VN+LIFLDV KAL DGMKLY+SDNKVILTEGFDGVV VKYF+KIESWP+RQ I
Sbjct: 241 ISGMRRDVNLLIFLDVRKALEDGMKLYISDNKVILTEGFDGVVPVKYFEKIESWPDRQTI 300

The following BLAST results are available for this feature:
BLAST of Cp4.1LG15g02830 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TRPT1_DANRE4.8e-3437.83tRNA 2'-phosphotransferase 1 OS=Danio rerio GN=trpt1 PE=2 SV=2[more]
TPT1_SCHPO4.5e-3238.54Putative tRNA 2'-phosphotransferase OS=Schizosaccharomyces pombe (strain 972 / A... [more]
TRPT1_MOUSE1.9e-3039.91tRNA 2'-phosphotransferase 1 OS=Mus musculus GN=Trpt1 PE=2 SV=2[more]
TRPT1_BOVIN1.1e-2737.50tRNA 2'-phosphotransferase 1 OS=Bos taurus GN=TRPT1 PE=2 SV=3[more]
TPT1_YEAST3.4e-1936.10tRNA 2'-phosphotransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S28... [more]
BLAST of Cp4.1LG15g02830 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L3T7_CUCSA3.4e-13585.67Uncharacterized protein OS=Cucumis sativus GN=Csa_3G126200 PE=4 SV=1[more]
D7U7X7_VITVI2.4e-11776.16Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0048g00300 PE=4 SV=... [more]
A0A0B2PYM4_GLYSO5.4e-11776.87tRNA 2'-phosphotransferase 1 OS=Glycine soja GN=glysoja_008635 PE=4 SV=1[more]
A0A151TBS2_CAJCA2.1e-11677.44tRNA 2'-phosphotransferase 1 OS=Cajanus cajan GN=KK1_019074 PE=4 SV=1[more]
K7MNY6_SOYBN1.0e-11575.85Uncharacterized protein OS=Glycine max GN=GLYMA_17G254400 PE=4 SV=1[more]
BLAST of Cp4.1LG15g02830 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G45330.11.9e-10575.90 RNA 2'-phosphotransferase, Tpt1 / KptA family[more]
AT5G23600.15.9e-9175.96 RNA 2'-phosphotransferase, Tpt1 / KptA family[more]
BLAST of Cp4.1LG15g02830 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778677447|ref|XP_004133917.2|4.8e-13585.67PREDICTED: tRNA 2'-phosphotransferase 1 [Cucumis sativus][more]
gi|659075488|ref|XP_008438171.1|6.3e-13586.01PREDICTED: tRNA 2'-phosphotransferase 1 [Cucumis melo][more]
gi|731421252|ref|XP_010661668.1|6.3e-11977.52PREDICTED: tRNA 2'-phosphotransferase 1 isoform X3 [Vitis vinifera][more]
gi|731421250|ref|XP_010661667.1|7.0e-11876.49PREDICTED: tRNA 2'-phosphotransferase 1 isoform X2 [Vitis vinifera][more]
gi|225454024|ref|XP_002274805.1|3.5e-11776.16PREDICTED: tRNA 2'-phosphotransferase 1 isoform X1 [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016772transferase activity, transferring phosphorus-containing groups
Vocabulary: Biological Process
TermDefinition
GO:0006388tRNA splicing, via endonucleolytic cleavage and ligation
Vocabulary: INTERPRO
TermDefinition
IPR002745Ptrans_KptA/Tpt1
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation
cellular_component GO:0005575 cellular_component
molecular_function GO:0000215 tRNA 2'-phosphotransferase activity
molecular_function GO:0016772 transferase activity, transferring phosphorus-containing groups
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG15g02830.1Cp4.1LG15g02830.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002745Phosphotransferase KptA/Tpt1PFAMPF01885PTS_2-RNAcoord: 76..256
score: 1.8
NoneNo IPR availablePANTHERPTHR12684PUTATIVE PHOSPHOTRANSFERASEcoord: 31..280
score: 3.7E
NoneNo IPR availablePANTHERPTHR12684:SF2TRNA 2'-PHOSPHOTRANSFERASE 1coord: 31..280
score: 3.7E
NoneNo IPR availableunknownSSF56399ADP-ribosylationcoord: 78..259
score: 2.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG15g02830CmoCh19G003580Cucurbita moschata (Rifu)cmocpeB481
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG15g02830Cucurbita maxima (Rimu)cmacpeB522