Cp4.1LG14g03010 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG14g03010
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG14 : 2627811 .. 2629761 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCATAATAACCTCCTCGTTAACATTGCCCTCTCGTCTCTCGTTCCTCTTTTTCTCGCTTTGCTCCTCGTCGTCATTCCTACCCCTCCTTTCCTCACTGTCCCCGACGATTCCCTCAGCTGCCACCACCACCACCGTAAACCCTCAGCTGATGAAGAAGACCGAGCGAGTATTGGTTCCATTTGTGACACCTTCCCGATTGCCCTTCTCCCACCAGAAACTGCCAACACTTCTGTCTTATGCGTTGACCAAAATGGATGCTGCAACTTCACTACTGTACAGGCAGCTGTCGATGCTGTGTTGAATATGAGCTCCAAACGAAGCTTAATATGGATCAACACTGGAATCTACTTGTAAACTCTTTATATAAATAACACCTGTTGTAAATTACAAAAATACCCTTTGACTTTGTTTTTGTTTTATAACTACCCTTATTTATAGATGATAAGACGAGATTGACCTACATGGAACGTGATGTTTTGTTGTTTTTTTTTAGTGAGAAGGTGATGGTTCCGAGATTGAAACCGAACGTGACATTTCAAGGACAAGGCTACACAACCACGGCTATTGTTTGGAACGACACCGCCAATTCTTCGCATGGCACATTTTACAGCGGCTCCGTCCAAGTCTTCTCTTCCAATTTTATTGCCAAGAACATAAGCTTCATGGTAATTTCTAATTAATTATAAATTATAACTTTTTTTAATCTTAGATTATAAACTCATGATCATCTTCTAAATTAGCTGAGGTGGGACTTTTATCATCCAACCCCTCCCCTTAAACAAAGTATGTCTCCCCTTAGTCGAGGCTGGGCTCCTTTGAAGTCTTAGTTATTTTTTACTGCCTTTGAGTAGGCTTGACTCCTTTTCTTTTGGAGCCATTTGTTCGATATTTGAGAATTTACCAATCTATTGGCACGAGTAAGTATAGAGCTTGACTCTAATACTATGTCAGACCAAGATAACTCTCATGTCTTTGCTTTGAGCTTCCTAAAACAATGGGCATTGGATTTTCATCCTCCAATGTGTATTAAATTAACCAATAAATTTTAAATCTAAAAAAGAAATTCCCAAACCCATAGAAAGCAAAATAAATACTCCCCGCGAAATTATAAGTAAACATTTTGGTGCCTTGGCAGAATGTGGCGCCAATTCCAGGGCCGGGCGTCATCGGAGCACAGGCGGTGGCGATTCGGATCGGCGGAGACCAGGCGGCGTTTTGGAACTGCGGATTCTTTGGCGCTCAAGACACTCTCCACGATGACCGCGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGAAACGGAAGATCCTACTACGAGGTAAAAATCGGAGCCCTAGTTTTTTCGCCGATTTCGATTTGACGATAAATTTCTGAATCGCGATTATGAAAACCTTGCCGCATTGTAGAGTTGCCAGATGATTTCGATGGCGAATCCAGTTCCGCAAGGATCAAAGTTCGTGAACGGAGCCGTGACGGCGCACGGCAGAGCCTCCGCAGACGAGAACTCTGGATTTGCTTTTGTTAATTGTAGCATCGGTGGAACCGGCCGAGTGTGGCTCGGTCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTTGCTAACACCGTCATGACGGATATTATTGCGTCGGAGGGATGGAATGACTTTAATGACCCCGCAAGGGACCAGTACGTTTTCTTTTTTCTAATTACGAAATTATCCACTAAATTTAAAACCATAATTATTATTTAAGGAATTTTGATAATGGAAAAAAATTGAAATTTGCAGGACGATCTTCTATGGGGAGTACAACTGCAGTGGAGCAGGAGCCAACATGAGCGCAAGGGCTCCCTATGTTCAGAGGCTCAATGATACTCAAGCTTCCCCTTTCCTCAACCTCTCCTTCATTGATGCTGACCAATGGCTTCAACCCTATTAGTTCT

mRNA sequence

ATGAATCATAATAACCTCCTCGTTAACATTGCCCTCTCGTCTCTCGTTCCTCTTTTTCTCGCTTTGCTCCTCGTCGTCATTCCTACCCCTCCTTTCCTCACTGTCCCCGACGATTCCCTCAGCTGCCACCACCACCACCGTAAACCCTCAGCTGATGAAGAAGACCGAGCGAGTATTGGTTCCATTTGTGACACCTTCCCGATTGCCCTTCTCCCACCAGAAACTGCCAACACTTCTGTCTTATGCGTTGACCAAAATGGATGCTGCAACTTCACTACTGTACAGGCAGCTGTCGATGCTGTGTTGAATATGAGCTCCAAACGAAGCTTAATATGGATCAACACTGGAATCTACTTTGAGAAGGTGATGGTTCCGAGATTGAAACCGAACGTGACATTTCAAGGACAAGGCTACACAACCACGGCTATTGTTTGGAACGACACCGCCAATTCTTCGCATGGCACATTTTACAGCGGCTCCGTCCAAAATGTGGCGCCAATTCCAGGGCCGGGCGTCATCGGAGCACAGGCGGTGGCGATTCGGATCGGCGGAGACCAGGCGGCGTTTTGGAACTGCGGATTCTTTGGCGCTCAAGACACTCTCCACGATGACCGCGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGAAACGGAAGATCCTACTACGAGAGTTGCCAGATGATTTCGATGGCGAATCCAGTTCCGCAAGGATCAAAGTTCGTGAACGGAGCCGTGACGGCGCACGGCAGAGCCTCCGCAGACGAGAACTCTGGATTTGCTTTTGTTAATTGTAGCATCGGTGGAACCGGCCGAGTGTGGCTCGGTCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTTGCTAACACCGTCATGACGGATATTATTGCGTCGGAGGGATGGAATGACTTTAATGACCCCGCAAGGGACCAGACGATCTTCTATGGGGAGTACAACTGCAGTGGAGCAGGAGCCAACATGAGCGCAAGGGCTCCCTATGTTCAGAGGCTCAATGATACTCAAGCTTCCCCTTTCCTCAACCTCTCCTTCATTGATGCTGACCAATGGCTTCAACCCTATTAGTTCT

Coding sequence (CDS)

ATGAATCATAATAACCTCCTCGTTAACATTGCCCTCTCGTCTCTCGTTCCTCTTTTTCTCGCTTTGCTCCTCGTCGTCATTCCTACCCCTCCTTTCCTCACTGTCCCCGACGATTCCCTCAGCTGCCACCACCACCACCGTAAACCCTCAGCTGATGAAGAAGACCGAGCGAGTATTGGTTCCATTTGTGACACCTTCCCGATTGCCCTTCTCCCACCAGAAACTGCCAACACTTCTGTCTTATGCGTTGACCAAAATGGATGCTGCAACTTCACTACTGTACAGGCAGCTGTCGATGCTGTGTTGAATATGAGCTCCAAACGAAGCTTAATATGGATCAACACTGGAATCTACTTTGAGAAGGTGATGGTTCCGAGATTGAAACCGAACGTGACATTTCAAGGACAAGGCTACACAACCACGGCTATTGTTTGGAACGACACCGCCAATTCTTCGCATGGCACATTTTACAGCGGCTCCGTCCAAAATGTGGCGCCAATTCCAGGGCCGGGCGTCATCGGAGCACAGGCGGTGGCGATTCGGATCGGCGGAGACCAGGCGGCGTTTTGGAACTGCGGATTCTTTGGCGCTCAAGACACTCTCCACGATGACCGCGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGAAACGGAAGATCCTACTACGAGAGTTGCCAGATGATTTCGATGGCGAATCCAGTTCCGCAAGGATCAAAGTTCGTGAACGGAGCCGTGACGGCGCACGGCAGAGCCTCCGCAGACGAGAACTCTGGATTTGCTTTTGTTAATTGTAGCATCGGTGGAACCGGCCGAGTGTGGCTCGGTCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTTGCTAACACCGTCATGACGGATATTATTGCGTCGGAGGGATGGAATGACTTTAATGACCCCGCAAGGGACCAGACGATCTTCTATGGGGAGTACAACTGCAGTGGAGCAGGAGCCAACATGAGCGCAAGGGCTCCCTATGTTCAGAGGCTCAATGATACTCAAGCTTCCCCTTTCCTCAACCTCTCCTTCATTGATGCTGACCAATGGCTTCAACCCTATTAG

Protein sequence

MNHNNLLVNIALSSLVPLFLALLLVVIPTPPFLTVPDDSLSCHHHHRKPSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY
BLAST of Cp4.1LG14g03010 vs. Swiss-Prot
Match: PME8_ARATH (Probable pectinesterase 8 OS=Arabidopsis thaliana GN=PME8 PE=2 SV=2)

HSP 1 Score: 483.8 bits (1244), Expect = 1.7e-135
Identity = 227/337 (67.36%), Postives = 270/337 (80.12%), Query Frame = 1

Query: 43  HHHHRKPSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVL 102
           HHHHR   +D + + SI   CD FP  + P +T  TS LCVD+NGCCNFTTVQ+AVDAV 
Sbjct: 56  HHHHRHKPSDTKRKVSI---CDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVG 115

Query: 103 NMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 162
           N S +R++IWIN+G+Y+EKV++P+ KPN+T QGQG+  TAI WNDTA S++GTFY  +VQ
Sbjct: 116 NFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQ 175

Query: 163 --------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRH 222
                         NVAPIP PG +GAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRH
Sbjct: 176 VFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRH 235

Query: 223 YFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFA 282
           YFKDCYIQGSIDFIFGN +S Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGF+
Sbjct: 236 YFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFS 295

Query: 283 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCS 342
           FVNC+IGGTG VWLGRAWRP+SRVVF +T MTD+IA EGWN+FNDP+RD TIFYGEYNCS
Sbjct: 296 FVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCS 355

Query: 343 GAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQ 366
           G GA+MS RAPYVQ+LN+TQ +  +N SFID DQWLQ
Sbjct: 356 GPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

BLAST of Cp4.1LG14g03010 vs. Swiss-Prot
Match: PME15_ARATH (Probable pectinesterase 15 OS=Arabidopsis thaliana GN=PME15 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 5.6e-83
Identity = 166/381 (43.57%), Postives = 234/381 (61.42%), Query Frame = 1

Query: 12  LSSLVPLFLALLLVVIPTPPFLTVPDDSLSC-------------HHHHRKPSADEEDRAS 71
           L +L+ + LAL  + +P+   L+    ++               HHHH            
Sbjct: 10  LIALLSIILALYTIQLPSSRSLSSIATTIGALAKYSTFFGHKHHHHHHHHHHHHHYHHHE 69

Query: 72  IGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIY 131
               C+ +  + L  +   + VL VD +GC NF+ VQ+A+D V ++SS ++LI +N+G Y
Sbjct: 70  PIKCCEKWT-SRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSY 129

Query: 132 FEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTF--------------YSGSVQNV 191
            EKV V   K N+  QG+GY  T+I WNDTA S+  T               Y+ S +N 
Sbjct: 130 REKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNN 189

Query: 192 APIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFG 251
           AP P PG   AQAVA+RI GDQAAF+ CGF+GAQDTL DD+GRH+FK+C+IQGSIDFIFG
Sbjct: 190 APEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFG 249

Query: 252 NGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGR 311
           NGRS Y+ C + S+A     G   V G++TA GR S DE SGF+FVNC I G+G + LGR
Sbjct: 250 NGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGR 309

Query: 312 AWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRL 366
           AW  ++ VVF+NT M+ II  EGWN++ D  +++T+ +GE+ C G GA+   R  + ++L
Sbjct: 310 AWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQL 369

BLAST of Cp4.1LG14g03010 vs. Swiss-Prot
Match: PME14_ARATH (Putative pectinesterase 14 OS=Arabidopsis thaliana GN=PME14 PE=2 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.6e-82
Identity = 148/306 (48.37%), Postives = 201/306 (65.69%), Query Frame = 1

Query: 73  PETANTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVT 132
           P    T VL V  NGC  F  VQ A+DA +  S  ++LI I+ GIY E+ +V   K N+ 
Sbjct: 34  PTKGFTMVLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLV 93

Query: 133 FQGQGYTTTAIVWNDTANSSHGTFYSGSV--------------QNVAPIPGPGVIGAQAV 192
            QG GY+ T+I WN+T  SS+GTF S SV              +N AP P PG + AQAV
Sbjct: 94  VQGMGYSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAV 153

Query: 193 AIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISM 252
           A+++ GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNGRS YE C + S+
Sbjct: 154 ALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSI 213

Query: 253 ANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTV 312
           A       +   G +TA+G+ +  + +GF FVNC I G+ RVWLGRAWRP++RV+F+ T 
Sbjct: 214 AK------ENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTY 273

Query: 313 MTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQASPFLNLSFI 365
           M+ +++ +GWND  DP   +T++YGE+ C G GAN S R  Y + L+D +A+PF N+SFI
Sbjct: 274 MSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFI 333

BLAST of Cp4.1LG14g03010 vs. Swiss-Prot
Match: PME53_ARATH (Probable pectinesterase 53 OS=Arabidopsis thaliana GN=PME53 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.4e-73
Identity = 134/296 (45.27%), Postives = 190/296 (64.19%), Query Frame = 1

Query: 90  NFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTA 149
           +FT +Q A+D++  ++  R +I ++ G+Y EKV +P LK  +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 150 NSSH------GTFYSGSV--------------QNVAPIPGPGVIGAQAVAIRIGGDQAAF 209
            +        GT+ S S               +N  P+P PG +G QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 210 WNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFV 269
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 270 NGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWN 329
            GAVTA GR+S  E++GF+FV C + GTG ++LGRAW PFSRVVFA T M +II   GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 330 DFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQ 366
           ++ DP+R+ T+FYG+Y C+GAGAN   R  + + L D +A PFL+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Cp4.1LG14g03010 vs. Swiss-Prot
Match: PME68_ARATH (Probable pectinesterase 68 OS=Arabidopsis thaliana GN=PME68 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.8e-65
Identity = 145/354 (40.96%), Postives = 199/354 (56.21%), Query Frame = 1

Query: 37  DDSLSCHHHHRK---PSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTT 96
           DD    HHHHRK   PS  +                          V+ V  NG   F +
Sbjct: 40  DDQQIQHHHHRKWVGPSGHK--------------------------VITVSLNGHAQFRS 99

Query: 97  VQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANS-- 156
           VQ AVD++   ++K   I I  G Y EKV+VP  KP +TF+G G   TAI W+D A+   
Sbjct: 100 VQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRASDLG 159

Query: 157 SHG----TFYSGSVQ--------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCG 216
           ++G    T+ + SV               N AP P PG+ G QAVA RI GD+A F  CG
Sbjct: 160 ANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFSGCG 219

Query: 217 FFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAV 276
           F+GAQDTL DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C++ S+A      S+F  G++
Sbjct: 220 FYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA------SRF--GSI 279

Query: 277 TAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFND 336
            AHGR   +E +GFAFV C + GTG +++GRA   +SR+V+A T    ++A  GW+D++ 
Sbjct: 280 AAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDH 339

Query: 337 PA-RDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQP 367
            + + +T F+G YNC G GA  +    + + L+   A PF+  SF++   W+ P
Sbjct: 340 KSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIAP 359

BLAST of Cp4.1LG14g03010 vs. TrEMBL
Match: A0A0A0KVA9_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G056710 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 7.5e-175
Identity = 305/390 (78.21%), Postives = 332/390 (85.13%), Query Frame = 1

Query: 1   MNHNNLLVNIALSSLVPLFLALLLVVIPTPPFLT---VPDDSLSC------HHHHRKPSA 60
           MN+N+ L+N+ LSSL+PL LA L ++ PTP FL+   V  D + C      HHHH  P  
Sbjct: 5   MNYNHFLINLTLSSLLPL-LAFLFMINPTPSFLSNLVVFGDPVDCPHHHHPHHHHENP-- 64

Query: 61  DEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLI 120
           DE+D++ I SICD FP AL+P ET NTS+ CVDQN CCNFTT+QAAVDAV N+S KR++I
Sbjct: 65  DEDDQSVISSICDNFPTALVPLETTNTSIFCVDQNSCCNFTTIQAAVDAVPNLSIKRNII 124

Query: 121 WINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ--------- 180
           WIN GIY+EKVMVP+ K NVTFQGQGYTTTAIVWNDTANSSHGTFYS SVQ         
Sbjct: 125 WINAGIYYEKVMVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFIAK 184

Query: 181 -----NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQG 240
                NVAPIP PG +GAQ VAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQG
Sbjct: 185 NLSFMNVAPIPAPGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQG 244

Query: 241 SIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGT 300
           SIDFIFGNGRS+YESCQ++SMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGT
Sbjct: 245 SIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGT 304

Query: 301 GRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSAR 360
           GRVWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSGAGANMS+R
Sbjct: 305 GRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANMSSR 364

Query: 361 APYVQRLNDTQASPFLNLSFIDADQWLQPY 368
           APYVQRLNDTQ S FLNLSFIDADQWLQPY
Sbjct: 365 APYVQRLNDTQVSSFLNLSFIDADQWLQPY 391

BLAST of Cp4.1LG14g03010 vs. TrEMBL
Match: A0A068UK07_COFCA (Pectinesterase OS=Coffea canephora GN=GSCOC_T00027982001 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 4.9e-150
Identity = 249/339 (73.45%), Postives = 284/339 (83.78%), Query Frame = 1

Query: 43  HHHHRKPSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVL 102
           HHHH      ++ +  I SICD FP    PP+T  TS +CVD NGCCNFTTVQAAVDAV 
Sbjct: 61  HHHHH-----DDKKRKIVSICDDFPPDFPPPDTNTTSTICVDHNGCCNFTTVQAAVDAVE 120

Query: 103 NMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 162
            +SSKR++IWIN GIYFEKV++P+ KPN+TFQGQG+T+TAIVWNDTANSSHGTFYSGSVQ
Sbjct: 121 VLSSKRTIIWINNGIYFEKVIIPKTKPNITFQGQGFTSTAIVWNDTANSSHGTFYSGSVQ 180

Query: 163 --------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRH 222
                         NVAPIPGPG +GAQAVA+RI GDQAAFW CGFFGAQDTLHDDRGRH
Sbjct: 181 VFAANFIAKNISFMNVAPIPGPGAMGAQAVAMRIAGDQAAFWGCGFFGAQDTLHDDRGRH 240

Query: 223 YFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFA 282
           YFK+CYIQGSIDF+FGNG+S+YE+CQ+ S+ANPV  G++ + GAVTAHGRAS DENSG+A
Sbjct: 241 YFKECYIQGSIDFVFGNGKSFYENCQLTSIANPVAVGARAITGAVTAHGRASKDENSGYA 300

Query: 283 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCS 342
           FVNCSIGGTGR+WLGRAWRPFS VVFA T M+DIIASEGWNDFNDP+RDQT+FYGEYNCS
Sbjct: 301 FVNCSIGGTGRIWLGRAWRPFSTVVFAYTSMSDIIASEGWNDFNDPSRDQTVFYGEYNCS 360

Query: 343 GAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 368
           G GAN + R PY Q+LNDTQA+PFLN+SFID DQWLQPY
Sbjct: 361 GNGANTTLRVPYAQKLNDTQATPFLNISFIDGDQWLQPY 394

BLAST of Cp4.1LG14g03010 vs. TrEMBL
Match: A0A0D2QHG2_GOSRA (Pectinesterase OS=Gossypium raimondii GN=B456_001G093600 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 2.4e-149
Identity = 257/347 (74.06%), Postives = 285/347 (82.13%), Query Frame = 1

Query: 35  VPDDSLSCHHHHRKPSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTV 94
           +P D      H + P     D+    S+CD FP  + PPET  T  LCVDQNGCCNFTTV
Sbjct: 52  IPFDFSDFCRHRKHP-----DKGKPVSVCDDFPPNIPPPETNTTLTLCVDQNGCCNFTTV 111

Query: 95  QAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHG 154
           QAAVDAV N+S KRS+IWINTGIY+EKV++P+ KPN+TFQGQGYT+TAI WNDTANS++G
Sbjct: 112 QAAVDAVPNLSMKRSVIWINTGIYYEKVIIPKTKPNITFQGQGYTSTAIAWNDTANSANG 171

Query: 155 TFYSGSVQ--------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDT 214
           TFYSGSVQ              NVAP+P PG +GAQAVAIRI GDQAAFW CGF+GAQDT
Sbjct: 172 TFYSGSVQVFSTNFIAKNMSFMNVAPLPRPGDVGAQAVAIRIAGDQAAFWGCGFYGAQDT 231

Query: 215 LHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRAS 274
           LHDDRGRHYFKDCYIQGSIDFIFGNG+S YE CQ+ISMAN V  GSK +NGAVTAHGRAS
Sbjct: 232 LHDDRGRHYFKDCYIQGSIDFIFGNGKSLYEDCQLISMANQVAPGSKSINGAVTAHGRAS 291

Query: 275 ADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTI 334
           ADE+SGFAFVNCSIGGTGR+WLGRAWRP+SRVVFA T MTDIIA EGWNDFNDP+RDQTI
Sbjct: 292 ADEDSGFAFVNCSIGGTGRIWLGRAWRPYSRVVFALTSMTDIIAPEGWNDFNDPSRDQTI 351

Query: 335 FYGEYNCSGAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 368
           FYGEYNC+GAGANM+ RAPYVQ+LNDTQAS FL  SFIDADQWLQ Y
Sbjct: 352 FYGEYNCTGAGANMNGRAPYVQKLNDTQASLFLTASFIDADQWLQSY 393

BLAST of Cp4.1LG14g03010 vs. TrEMBL
Match: A0A0B0MV28_GOSAR (Pectinesterase OS=Gossypium arboreum GN=F383_27254 PE=4 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 5.4e-149
Identity = 257/347 (74.06%), Postives = 284/347 (81.84%), Query Frame = 1

Query: 35  VPDDSLSCHHHHRKPSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTV 94
           +P D      H + P     D+    S+CD FP    PPET  T  LCVD+NGCCNFTTV
Sbjct: 4   IPFDFSDFCRHRKHP-----DKGKPVSVCDDFPPNFPPPETNTTLTLCVDRNGCCNFTTV 63

Query: 95  QAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHG 154
           QAAVDAV N+S KRS+IWINTGIY+EKV++P+ KPN+TFQGQGYT+TAI WNDTANS++G
Sbjct: 64  QAAVDAVPNLSMKRSVIWINTGIYYEKVIIPKTKPNITFQGQGYTSTAIAWNDTANSANG 123

Query: 155 TFYSGSVQ--------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDT 214
           TFYSGSVQ              NVAP+P PG +GAQAVAIRI GDQAAFW CGF+GAQDT
Sbjct: 124 TFYSGSVQVFSTNFIAKNISFMNVAPLPRPGDVGAQAVAIRIAGDQAAFWGCGFYGAQDT 183

Query: 215 LHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRAS 274
           LHDDRGRHYFKDCYIQGSIDFIFGNG+S YE CQ+ISMAN V  GSK +NGAVTAHGRAS
Sbjct: 184 LHDDRGRHYFKDCYIQGSIDFIFGNGKSLYEDCQLISMANQVAPGSKSINGAVTAHGRAS 243

Query: 275 ADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTI 334
           ADE+SGFAFVNCSIGGTGR+WLGRAWRP+SRVVFA T MTDIIA EGWNDFNDP+RDQTI
Sbjct: 244 ADEDSGFAFVNCSIGGTGRIWLGRAWRPYSRVVFALTSMTDIIAPEGWNDFNDPSRDQTI 303

Query: 335 FYGEYNCSGAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 368
           FYGEYNC+GAGANMS RAPYVQ+LNDTQAS FL  SFIDADQWLQ Y
Sbjct: 304 FYGEYNCTGAGANMSGRAPYVQKLNDTQASLFLTTSFIDADQWLQSY 345

BLAST of Cp4.1LG14g03010 vs. TrEMBL
Match: A0A061DLD1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_002054 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 1.2e-148
Identity = 253/341 (74.19%), Postives = 281/341 (82.40%), Query Frame = 1

Query: 41  SCHHHHRKPSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDA 100
           S H HHRK      D+   GSICD FP    PPET  T  LCVD+NGCCNFTTVQ+AVDA
Sbjct: 58  SLHRHHRK----HPDKGKTGSICDDFPPNFPPPETNTTLTLCVDRNGCCNFTTVQSAVDA 117

Query: 101 VLNMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGS 160
           V N S +R++IWIN+GIY+EKV +P+ KPN+TFQGQGYT+TA+VWNDTANS++GTFYSGS
Sbjct: 118 VANFSMRRTVIWINSGIYYEKVTIPKTKPNITFQGQGYTSTAVVWNDTANSANGTFYSGS 177

Query: 161 VQ--------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRG 220
           VQ              NVAPI  PG +GAQAVAIRI GDQAAFW CGFFGAQDTLHDDRG
Sbjct: 178 VQVFSANFIAKNISFMNVAPIARPGDVGAQAVAIRIAGDQAAFWGCGFFGAQDTLHDDRG 237

Query: 221 RHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSG 280
           RHYFKDCYIQGSIDFIFGNGRS YE+CQ++SMANPV  GSK +NGAVTAHGRAS DEN+G
Sbjct: 238 RHYFKDCYIQGSIDFIFGNGRSLYENCQLVSMANPVAPGSKGINGAVTAHGRASGDENTG 297

Query: 281 FAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYN 340
           FAFVNCS+GGTGR+WLGRAWRPFSRVVFA T MTDIIA EGWNDFNDPARDQTI YGEYN
Sbjct: 298 FAFVNCSVGGTGRIWLGRAWRPFSRVVFALTSMTDIIAPEGWNDFNDPARDQTISYGEYN 357

Query: 341 CSGAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 368
           C+G GAN + RAPYVQ+L+DTQAS FLN S+ID DQWLQ Y
Sbjct: 358 CTGDGANTTLRAPYVQKLDDTQASIFLNTSYIDGDQWLQSY 394

BLAST of Cp4.1LG14g03010 vs. TAIR10
Match: AT1G05310.1 (AT1G05310.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 483.8 bits (1244), Expect = 9.4e-137
Identity = 227/337 (67.36%), Postives = 270/337 (80.12%), Query Frame = 1

Query: 43  HHHHRKPSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVL 102
           HHHHR   +D + + SI   CD FP  + P +T  TS LCVD+NGCCNFTTVQ+AVDAV 
Sbjct: 56  HHHHRHKPSDTKRKVSI---CDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVG 115

Query: 103 NMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 162
           N S +R++IWIN+G+Y+EKV++P+ KPN+T QGQG+  TAI WNDTA S++GTFY  +VQ
Sbjct: 116 NFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQ 175

Query: 163 --------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRH 222
                         NVAPIP PG +GAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRH
Sbjct: 176 VFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRH 235

Query: 223 YFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFA 282
           YFKDCYIQGSIDFIFGN +S Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGF+
Sbjct: 236 YFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFS 295

Query: 283 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCS 342
           FVNC+IGGTG VWLGRAWRP+SRVVF +T MTD+IA EGWN+FNDP+RD TIFYGEYNCS
Sbjct: 296 FVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCS 355

Query: 343 GAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQ 366
           G GA+MS RAPYVQ+LN+TQ +  +N SFID DQWLQ
Sbjct: 356 GPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

BLAST of Cp4.1LG14g03010 vs. TAIR10
Match: AT2G36710.1 (AT2G36710.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 309.3 bits (791), Expect = 3.2e-84
Identity = 166/381 (43.57%), Postives = 234/381 (61.42%), Query Frame = 1

Query: 12  LSSLVPLFLALLLVVIPTPPFLTVPDDSLSC-------------HHHHRKPSADEEDRAS 71
           L +L+ + LAL  + +P+   L+    ++               HHHH            
Sbjct: 10  LIALLSIILALYTIQLPSSRSLSSIATTIGALAKYSTFFGHKHHHHHHHHHHHHHYHHHE 69

Query: 72  IGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIY 131
               C+ +  + L  +   + VL VD +GC NF+ VQ+A+D V ++SS ++LI +N+G Y
Sbjct: 70  PIKCCEKWT-SRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSY 129

Query: 132 FEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTF--------------YSGSVQNV 191
            EKV V   K N+  QG+GY  T+I WNDTA S+  T               Y+ S +N 
Sbjct: 130 REKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNN 189

Query: 192 APIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFG 251
           AP P PG   AQAVA+RI GDQAAF+ CGF+GAQDTL DD+GRH+FK+C+IQGSIDFIFG
Sbjct: 190 APEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFG 249

Query: 252 NGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGR 311
           NGRS Y+ C + S+A     G   V G++TA GR S DE SGF+FVNC I G+G + LGR
Sbjct: 250 NGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGR 309

Query: 312 AWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRL 366
           AW  ++ VVF+NT M+ II  EGWN++ D  +++T+ +GE+ C G GA+   R  + ++L
Sbjct: 310 AWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQL 369

BLAST of Cp4.1LG14g03010 vs. TAIR10
Match: AT2G36700.1 (AT2G36700.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 307.8 bits (787), Expect = 9.2e-84
Identity = 148/306 (48.37%), Postives = 201/306 (65.69%), Query Frame = 1

Query: 73  PETANTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVT 132
           P    T VL V  NGC  F  VQ A+DA +  S  ++LI I+ GIY E+ +V   K N+ 
Sbjct: 34  PTKGFTMVLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLV 93

Query: 133 FQGQGYTTTAIVWNDTANSSHGTFYSGSV--------------QNVAPIPGPGVIGAQAV 192
            QG GY+ T+I WN+T  SS+GTF S SV              +N AP P PG + AQAV
Sbjct: 94  VQGMGYSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAV 153

Query: 193 AIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISM 252
           A+++ GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNGRS YE C + S+
Sbjct: 154 ALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSI 213

Query: 253 ANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTV 312
           A       +   G +TA+G+ +  + +GF FVNC I G+ RVWLGRAWRP++RV+F+ T 
Sbjct: 214 AK------ENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTY 273

Query: 313 MTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQASPFLNLSFI 365
           M+ +++ +GWND  DP   +T++YGE+ C G GAN S R  Y + L+D +A+PF N+SFI
Sbjct: 274 MSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFI 333

BLAST of Cp4.1LG14g03010 vs. TAIR10
Match: AT5G19730.1 (AT5G19730.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 277.3 bits (708), Expect = 1.3e-74
Identity = 134/296 (45.27%), Postives = 190/296 (64.19%), Query Frame = 1

Query: 90  NFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTA 149
           +FT +Q A+D++  ++  R +I ++ G+Y EKV +P LK  +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 150 NSSH------GTFYSGSV--------------QNVAPIPGPGVIGAQAVAIRIGGDQAAF 209
            +        GT+ S S               +N  P+P PG +G QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 210 WNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFV 269
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 270 NGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWN 329
            GAVTA GR+S  E++GF+FV C + GTG ++LGRAW PFSRVVFA T M +II   GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 330 DFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQ 366
           ++ DP+R+ T+FYG+Y C+GAGAN   R  + + L D +A PFL+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Cp4.1LG14g03010 vs. TAIR10
Match: AT5G47500.1 (AT5G47500.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 251.1 bits (640), Expect = 1.0e-66
Identity = 145/354 (40.96%), Postives = 199/354 (56.21%), Query Frame = 1

Query: 37  DDSLSCHHHHRK---PSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTT 96
           DD    HHHHRK   PS  +                          V+ V  NG   F +
Sbjct: 40  DDQQIQHHHHRKWVGPSGHK--------------------------VITVSLNGHAQFRS 99

Query: 97  VQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANS-- 156
           VQ AVD++   ++K   I I  G Y EKV+VP  KP +TF+G G   TAI W+D A+   
Sbjct: 100 VQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRASDLG 159

Query: 157 SHG----TFYSGSVQ--------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCG 216
           ++G    T+ + SV               N AP P PG+ G QAVA RI GD+A F  CG
Sbjct: 160 ANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFSGCG 219

Query: 217 FFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAV 276
           F+GAQDTL DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C++ S+A      S+F  G++
Sbjct: 220 FYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA------SRF--GSI 279

Query: 277 TAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFND 336
            AHGR   +E +GFAFV C + GTG +++GRA   +SR+V+A T    ++A  GW+D++ 
Sbjct: 280 AAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDH 339

Query: 337 PA-RDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQP 367
            + + +T F+G YNC G GA  +    + + L+   A PF+  SF++   W+ P
Sbjct: 340 KSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIAP 359

BLAST of Cp4.1LG14g03010 vs. NCBI nr
Match: gi|659101970|ref|XP_008451885.1| (PREDICTED: probable pectinesterase 8 [Cucumis melo])

HSP 1 Score: 630.6 bits (1625), Expect = 1.8e-177
Identity = 306/385 (79.48%), Postives = 333/385 (86.49%), Query Frame = 1

Query: 3   HNNLLVNIALSSLVPLFLALLLVVIPTPPFLT---VPDDSLSC---HHHHRKPSADEEDR 62
           +NN L+N+ LSSL+PL LA L ++ PTP +L+   V  D + C   HHHH     DE+D+
Sbjct: 8   YNNFLINLTLSSLLPL-LAFLFMINPTPSYLSNLVVFGDPVDCPHPHHHHHHEDPDEDDQ 67

Query: 63  ASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTG 122
           + I SICD FP  L+PPET NTSV CVDQNGCCNFTTVQAAVDAV N+S KR++IWIN G
Sbjct: 68  SVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVPNLSIKRNIIWINAG 127

Query: 123 IYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ-------------- 182
           IY+EKVMVP+ KPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ              
Sbjct: 128 IYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFM 187

Query: 183 NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFI 242
           NVAPIPGPG +GAQAVAIR+GGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFI
Sbjct: 188 NVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFI 247

Query: 243 FGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWL 302
           FGNGRS+YESCQ++SMANPVPQGSKFVNGAVTAHGRASA+ENSGF+FVNCSIGGTGRVWL
Sbjct: 248 FGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSFVNCSIGGTGRVWL 307

Query: 303 GRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQ 362
           GRAWRPFSRVVFANTVMTDIIA EGWNDFNDP RDQTIFYGEYNCSGAGANMS+RAPYVQ
Sbjct: 308 GRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSGAGANMSSRAPYVQ 367

Query: 363 RLNDTQASPFLNLSFIDADQWLQPY 368
           RLNDTQ S FLNLSFIDADQWLQPY
Sbjct: 368 RLNDTQVSSFLNLSFIDADQWLQPY 391

BLAST of Cp4.1LG14g03010 vs. NCBI nr
Match: gi|449460078|ref|XP_004147773.1| (PREDICTED: probable pectinesterase 8 [Cucumis sativus])

HSP 1 Score: 621.3 bits (1601), Expect = 1.1e-174
Identity = 305/390 (78.21%), Postives = 332/390 (85.13%), Query Frame = 1

Query: 1   MNHNNLLVNIALSSLVPLFLALLLVVIPTPPFLT---VPDDSLSC------HHHHRKPSA 60
           MN+N+ L+N+ LSSL+PL LA L ++ PTP FL+   V  D + C      HHHH  P  
Sbjct: 5   MNYNHFLINLTLSSLLPL-LAFLFMINPTPSFLSNLVVFGDPVDCPHHHHPHHHHENP-- 64

Query: 61  DEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLI 120
           DE+D++ I SICD FP AL+P ET NTS+ CVDQN CCNFTT+QAAVDAV N+S KR++I
Sbjct: 65  DEDDQSVISSICDNFPTALVPLETTNTSIFCVDQNSCCNFTTIQAAVDAVPNLSIKRNII 124

Query: 121 WINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ--------- 180
           WIN GIY+EKVMVP+ K NVTFQGQGYTTTAIVWNDTANSSHGTFYS SVQ         
Sbjct: 125 WINAGIYYEKVMVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFIAK 184

Query: 181 -----NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQG 240
                NVAPIP PG +GAQ VAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQG
Sbjct: 185 NLSFMNVAPIPAPGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQG 244

Query: 241 SIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGT 300
           SIDFIFGNGRS+YESCQ++SMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGT
Sbjct: 245 SIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGT 304

Query: 301 GRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSAR 360
           GRVWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSGAGANMS+R
Sbjct: 305 GRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANMSSR 364

Query: 361 APYVQRLNDTQASPFLNLSFIDADQWLQPY 368
           APYVQRLNDTQ S FLNLSFIDADQWLQPY
Sbjct: 365 APYVQRLNDTQVSSFLNLSFIDADQWLQPY 391

BLAST of Cp4.1LG14g03010 vs. NCBI nr
Match: gi|1009127798|ref|XP_015880886.1| (PREDICTED: probable pectinesterase 8 [Ziziphus jujuba])

HSP 1 Score: 546.6 bits (1407), Expect = 3.3e-152
Identity = 255/342 (74.56%), Postives = 284/342 (83.04%), Query Frame = 1

Query: 40  LSCHHHHRKPSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVD 99
           +S HHHH      + D     SICD FP    PP+T  TS+LCVD+NGCCNFTTVQAAVD
Sbjct: 62  ISRHHHHHHHHKKKPDGGKNVSICDDFPPNFPPPDTDTTSILCVDRNGCCNFTTVQAAVD 121

Query: 100 AVLNMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSG 159
           AV N S KR++IWIN GIY+EKV+VP +KPN+TFQGQG+T+TAIVWNDTANSSHGTFYSG
Sbjct: 122 AVSNSSQKRNIIWINVGIYYEKVIVPIIKPNITFQGQGFTSTAIVWNDTANSSHGTFYSG 181

Query: 160 SVQ--------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDR 219
           SVQ              NVAPIPGPG +GAQAVAIRI GDQAAFW CGFFGAQDTLHDD+
Sbjct: 182 SVQVFSDNFIAKNISFMNVAPIPGPGYMGAQAVAIRIAGDQAAFWGCGFFGAQDTLHDDK 241

Query: 220 GRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENS 279
           GRHYFK CYIQGSIDFIFGN RS YE CQ++SMANPV  GSK ++G+VTAHGR S DEN+
Sbjct: 242 GRHYFKQCYIQGSIDFIFGNARSLYEDCQVVSMANPVAAGSKSIDGSVTAHGRTSKDENT 301

Query: 280 GFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEY 339
           GF FVNC++GGTGRVWLGRAWRPFSRVVFA+T M+DIIA EGWNDFNDP RDQ+IFYGEY
Sbjct: 302 GFVFVNCTVGGTGRVWLGRAWRPFSRVVFASTTMSDIIAPEGWNDFNDPTRDQSIFYGEY 361

Query: 340 NCSGAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 368
           NC+G GANMS RAPYVQRLNDTQA+PFLNLSFID DQWLQP+
Sbjct: 362 NCTGPGANMSKRAPYVQRLNDTQAAPFLNLSFIDGDQWLQPF 403

BLAST of Cp4.1LG14g03010 vs. NCBI nr
Match: gi|747053882|ref|XP_011073122.1| (PREDICTED: probable pectinesterase 8 [Sesamum indicum])

HSP 1 Score: 538.9 bits (1387), Expect = 7.0e-150
Identity = 260/379 (68.60%), Postives = 296/379 (78.10%), Query Frame = 1

Query: 3   HNNLLVNIALSSLVPLFLALLLVVIPTPPFLTVPDDSLSCHHHHRKPSADEEDRASIGSI 62
           H   L ++ +       L+ L  ++PTP    +P      HHHH    +DE  R +  +I
Sbjct: 28  HTLFLKHLLIHFNPQTLLSSLPFIVPTP----IPFGCRHSHHHHHHHPSDE--RKTTKTI 87

Query: 63  CDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKV 122
           CD FP    PP+T  TS+ CVD NGCCNFTTVQAAVDAV  MS KR++IWINTGIYFEKV
Sbjct: 88  CDDFPPDFPPPDTNTTSIFCVDSNGCCNFTTVQAAVDAVGVMSPKRTVIWINTGIYFEKV 147

Query: 123 MVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ--------------NVAPIP 182
           ++P+ KPN+TFQGQGYT+TAIVWNDTANSSHGTFYSGSVQ              NVAPIP
Sbjct: 148 IIPKTKPNITFQGQGYTSTAIVWNDTANSSHGTFYSGSVQVFAPNFIAKNISFMNVAPIP 207

Query: 183 GPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 242
           GPGV+GAQAVA+RI GDQAAFW CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN +S
Sbjct: 208 GPGVVGAQAVALRIAGDQAAFWGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKS 267

Query: 243 YYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 302
           +YE C + S+A+PV  G++ +NGAVTAHGRAS DENSGFAFVNCSIGGTGR+WLGRAWRP
Sbjct: 268 FYEGCHITSIASPVAPGARVINGAVTAHGRASKDENSGFAFVNCSIGGTGRIWLGRAWRP 327

Query: 303 FSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQ 362
           FS VVF+ T MTDIIA EGWNDFNDP RD +IFYGEYNC+G GAN + R PY QRLNDTQ
Sbjct: 328 FSTVVFSYTSMTDIIAPEGWNDFNDPTRDLSIFYGEYNCTGDGANTTLRVPYAQRLNDTQ 387

Query: 363 ASPFLNLSFIDADQWLQPY 368
           ASPFLN+SF+DADQWLQ Y
Sbjct: 388 ASPFLNVSFVDADQWLQSY 400

BLAST of Cp4.1LG14g03010 vs. NCBI nr
Match: gi|661887734|emb|CDP08865.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 538.9 bits (1387), Expect = 7.0e-150
Identity = 249/339 (73.45%), Postives = 284/339 (83.78%), Query Frame = 1

Query: 43  HHHHRKPSADEEDRASIGSICDTFPIALLPPETANTSVLCVDQNGCCNFTTVQAAVDAVL 102
           HHHH      ++ +  I SICD FP    PP+T  TS +CVD NGCCNFTTVQAAVDAV 
Sbjct: 61  HHHHH-----DDKKRKIVSICDDFPPDFPPPDTNTTSTICVDHNGCCNFTTVQAAVDAVE 120

Query: 103 NMSSKRSLIWINTGIYFEKVMVPRLKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 162
            +SSKR++IWIN GIYFEKV++P+ KPN+TFQGQG+T+TAIVWNDTANSSHGTFYSGSVQ
Sbjct: 121 VLSSKRTIIWINNGIYFEKVIIPKTKPNITFQGQGFTSTAIVWNDTANSSHGTFYSGSVQ 180

Query: 163 --------------NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRH 222
                         NVAPIPGPG +GAQAVA+RI GDQAAFW CGFFGAQDTLHDDRGRH
Sbjct: 181 VFAANFIAKNISFMNVAPIPGPGAMGAQAVAMRIAGDQAAFWGCGFFGAQDTLHDDRGRH 240

Query: 223 YFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFA 282
           YFK+CYIQGSIDF+FGNG+S+YE+CQ+ S+ANPV  G++ + GAVTAHGRAS DENSG+A
Sbjct: 241 YFKECYIQGSIDFVFGNGKSFYENCQLTSIANPVAVGARAITGAVTAHGRASKDENSGYA 300

Query: 283 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCS 342
           FVNCSIGGTGR+WLGRAWRPFS VVFA T M+DIIASEGWNDFNDP+RDQT+FYGEYNCS
Sbjct: 301 FVNCSIGGTGRIWLGRAWRPFSTVVFAYTSMSDIIASEGWNDFNDPSRDQTVFYGEYNCS 360

Query: 343 GAGANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 368
           G GAN + R PY Q+LNDTQA+PFLN+SFID DQWLQPY
Sbjct: 361 GNGANTTLRVPYAQKLNDTQATPFLNISFIDGDQWLQPY 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME8_ARATH1.7e-13567.36Probable pectinesterase 8 OS=Arabidopsis thaliana GN=PME8 PE=2 SV=2[more]
PME15_ARATH5.6e-8343.57Probable pectinesterase 15 OS=Arabidopsis thaliana GN=PME15 PE=2 SV=1[more]
PME14_ARATH1.6e-8248.37Putative pectinesterase 14 OS=Arabidopsis thaliana GN=PME14 PE=2 SV=1[more]
PME53_ARATH2.4e-7345.27Probable pectinesterase 53 OS=Arabidopsis thaliana GN=PME53 PE=2 SV=1[more]
PME68_ARATH1.8e-6540.96Probable pectinesterase 68 OS=Arabidopsis thaliana GN=PME68 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KVA9_CUCSA7.5e-17578.21Pectinesterase OS=Cucumis sativus GN=Csa_4G056710 PE=4 SV=1[more]
A0A068UK07_COFCA4.9e-15073.45Pectinesterase OS=Coffea canephora GN=GSCOC_T00027982001 PE=4 SV=1[more]
A0A0D2QHG2_GOSRA2.4e-14974.06Pectinesterase OS=Gossypium raimondii GN=B456_001G093600 PE=4 SV=1[more]
A0A0B0MV28_GOSAR5.4e-14974.06Pectinesterase OS=Gossypium arboreum GN=F383_27254 PE=4 SV=1[more]
A0A061DLD1_THECC1.2e-14874.19Pectinesterase OS=Theobroma cacao GN=TCM_002054 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G05310.19.4e-13767.36 Pectin lyase-like superfamily protein[more]
AT2G36710.13.2e-8443.57 Pectin lyase-like superfamily protein[more]
AT2G36700.19.2e-8448.37 Pectin lyase-like superfamily protein[more]
AT5G19730.11.3e-7445.27 Pectin lyase-like superfamily protein[more]
AT5G47500.11.0e-6640.96 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659101970|ref|XP_008451885.1|1.8e-17779.48PREDICTED: probable pectinesterase 8 [Cucumis melo][more]
gi|449460078|ref|XP_004147773.1|1.1e-17478.21PREDICTED: probable pectinesterase 8 [Cucumis sativus][more]
gi|1009127798|ref|XP_015880886.1|3.3e-15274.56PREDICTED: probable pectinesterase 8 [Ziziphus jujuba][more]
gi|747053882|ref|XP_011073122.1|7.0e-15068.60PREDICTED: probable pectinesterase 8 [Sesamum indicum][more]
gi|661887734|emb|CDP08865.1|7.0e-15073.45unnamed protein product [Coffea canephora][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g03010.1Cp4.1LG14g03010.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 82..360
score: 2.5
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 78..366
score: 4.32
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 79..366
score: 1.0
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 31..366
score: 3.7E
NoneNo IPR availablePANTHERPTHR31321:SF8PECTINESTERASE 8-RELATEDcoord: 31..366
score: 3.7E