Cp4.1LG13g10950 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG13g10950
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionAspartic proteinase nepenthesin-1
LocationCp4.1LG13 : 8895481 .. 8896767 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTCTTCCTCCGCCTGCTGCAGCTTCTTATCTGCTGCGTCTCTTTCAAACAGGGCCTCTGTTTTTCTGCGACTCAGACCATGGTTTTGCCCCTCAAAACACAGATGGGTGTCACTTCTCGGCCTTCCAATAAGCTCAGTTTTCACCATAATGTCACTTTGACTGTTTCCTTAACGCTTGGCTCGCCTCCTCAACCCGTTACTATGGTTCTCGATACAGGGAGTGAACTCTCATGGCTTCACTGCAAAAAAACCCCAAATTTGAACTCTGTTTTTAACCCACTTTCTTCCTCTTCTTACTCGCCAGTCCCCTGTGCTTCCCCTGTTTGCCGGACCCGAACCCGAGATTTACCCAACCCGGTTACCTGCGACCCAAAGAAACTCTGCCACGTCTTTGTCTCTTATGCCGACGCCTCGTCGCTCGAGGGCAATCTCGCGTCGGATACGTTTCGAGTCGGGTCATCGGCTCAACCCGGAACTTTTTTTGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAGGAGGACGCGAAGACCACTGGGCTGATGGGGATGAACAGGGGCTCGCTCTCGTTTGTCACCCAATTGGGTTTGCCCAAATTCTCTTATTGCATATCGGGTCGTGATTCTTCTGGGGTTCTGCTTTTCGGCGACGTGAGTCTTTCTTGGCTTGGGAATTTGACCTACACGCCTTTGGTTCAAATGTCTACGCCATTGCCGTATTACGACCGAGTAGCCTACACGGTCCAACTAGACGGAATCAGAGTAGGGAACAAAATTCTGGCACTCCCGAAGTCAATATTCGCACCGGATCACACCGGCGCCGGGCAAACCATGGTAGATTCAGGGACCCAGTTCACGTTTCTTCTGGGACCAGTGTACACGGCTTTAAAGAACGAGTTTCTGGTACAAACAAAGGGCATTTTGGTCCCACTGGGTGATCCAAATTTCGTGTTTCAAGGAGCGATGGACTTGTGCTACAGTGTACCCGAGAAACAGGGGAAACTGCCGCCACTGCCGGTAGTGAGTCTGATGTTCCGTGGGGCGGAGATGGTGGTTGGCGGGGAGGTGCTAATGTATAAAGTACCGGGAATGGTGAGGGGTGGTGACCAAGTGCATTGCTTGACGTTTGGGAATTCGGATTTGTTAGGAATAGAAGCATTTGTGATTGGGCATCATCATCAACAAAACGTGTGGATGGAATTCGACTTGGCGAAATCAAGGGTTGGATTTGTAGAGACGAGGTGTGATTTGGCGGGTGAGCGGCTGGGGCTGGGGCCTTAA

mRNA sequence

ATGGCCTTCTTCCTCCGCCTGCTGCAGCTTCTTATCTGCTGCGTCTCTTTCAAACAGGGCCTCTGTTTTTCTGCGACTCAGACCATGGTTTTGCCCCTCAAAACACAGATGGGTGTCACTTCTCGGCCTTCCAATAAGCTCAGTTTTCACCATAATGTCACTTTGACTGTTTCCTTAACGCTTGGCTCGCCTCCTCAACCCGTTACTATGGTTCTCGATACAGGGAGTGAACTCTCATGGCTTCACTGCAAAAAAACCCCAAATTTGAACTCTGTTTTTAACCCACTTTCTTCCTCTTCTTACTCGCCAGTCCCCTGTGCTTCCCCTGTTTGCCGGACCCGAACCCGAGATTTACCCAACCCGGTTACCTGCGACCCAAAGAAACTCTGCCACGTCTTTGTCTCTTATGCCGACGCCTCGTCGCTCGAGGGCAATCTCGCGTCGGATACGTTTCGAGTCGGGTCATCGGCTCAACCCGGAACTTTTTTTGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAGGAGGACGCGAAGACCACTGGGCTGATGGGGATGAACAGGGGCTCGCTCTCGTTTGTCACCCAATTGGGTTTGCCCAAATTCTCTTATTGCATATCGGGTCGTGATTCTTCTGGGGTTCTGCTTTTCGGCGACGTGAGTCTTTCTTGGCTTGGGAATTTGACCTACACGCCTTTGGTTCAAATGTCTACGCCATTGCCGTATTACGACCGAGTAGCCTACACGGTCCAACTAGACGGAATCAGAGTAGGGAACAAAATTCTGGCACTCCCGAAGTCAATATTCGCACCGGATCACACCGGCGCCGGGCAAACCATGGTAGATTCAGGGACCCAGTTCACGTTTCTTCTGGGACCAGTGTACACGGCTTTAAAGAACGAGTTTCTGGTACAAACAAAGGGCATTTTGGTCCCACTGGGTGATCCAAATTTCGTGTTTCAAGGAGCGATGGACTTGTGCTACAGTGTACCCGAGAAACAGGGGAAACTGCCGCCACTGCCGGTAGTGAGTCTGATGTTCCGTGGGGCGGAGATGGTGGTTGGCGGGGAGGTGCTAATGTATAAAGTACCGGGAATGGTGAGGGGTGGTGACCAAGTGCATTGCTTGACGTTTGGGAATTCGGATTTGTTAGGAATAGAAGCATTTGTGATTGGGCATCATCATCAACAAAACGTGTGGATGGAATTCGACTTGGCGAAATCAAGGGTTGGATTTGTAGAGACGAGGTGTGATTTGGCGGGTGAGCGGCTGGGGCTGGGGCCTTAA

Coding sequence (CDS)

ATGGCCTTCTTCCTCCGCCTGCTGCAGCTTCTTATCTGCTGCGTCTCTTTCAAACAGGGCCTCTGTTTTTCTGCGACTCAGACCATGGTTTTGCCCCTCAAAACACAGATGGGTGTCACTTCTCGGCCTTCCAATAAGCTCAGTTTTCACCATAATGTCACTTTGACTGTTTCCTTAACGCTTGGCTCGCCTCCTCAACCCGTTACTATGGTTCTCGATACAGGGAGTGAACTCTCATGGCTTCACTGCAAAAAAACCCCAAATTTGAACTCTGTTTTTAACCCACTTTCTTCCTCTTCTTACTCGCCAGTCCCCTGTGCTTCCCCTGTTTGCCGGACCCGAACCCGAGATTTACCCAACCCGGTTACCTGCGACCCAAAGAAACTCTGCCACGTCTTTGTCTCTTATGCCGACGCCTCGTCGCTCGAGGGCAATCTCGCGTCGGATACGTTTCGAGTCGGGTCATCGGCTCAACCCGGAACTTTTTTTGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAGGAGGACGCGAAGACCACTGGGCTGATGGGGATGAACAGGGGCTCGCTCTCGTTTGTCACCCAATTGGGTTTGCCCAAATTCTCTTATTGCATATCGGGTCGTGATTCTTCTGGGGTTCTGCTTTTCGGCGACGTGAGTCTTTCTTGGCTTGGGAATTTGACCTACACGCCTTTGGTTCAAATGTCTACGCCATTGCCGTATTACGACCGAGTAGCCTACACGGTCCAACTAGACGGAATCAGAGTAGGGAACAAAATTCTGGCACTCCCGAAGTCAATATTCGCACCGGATCACACCGGCGCCGGGCAAACCATGGTAGATTCAGGGACCCAGTTCACGTTTCTTCTGGGACCAGTGTACACGGCTTTAAAGAACGAGTTTCTGGTACAAACAAAGGGCATTTTGGTCCCACTGGGTGATCCAAATTTCGTGTTTCAAGGAGCGATGGACTTGTGCTACAGTGTACCCGAGAAACAGGGGAAACTGCCGCCACTGCCGGTAGTGAGTCTGATGTTCCGTGGGGCGGAGATGGTGGTTGGCGGGGAGGTGCTAATGTATAAAGTACCGGGAATGGTGAGGGGTGGTGACCAAGTGCATTGCTTGACGTTTGGGAATTCGGATTTGTTAGGAATAGAAGCATTTGTGATTGGGCATCATCATCAACAAAACGTGTGGATGGAATTCGACTTGGCGAAATCAAGGGTTGGATTTGTAGAGACGAGGTGTGATTTGGCGGGTGAGCGGCTGGGGCTGGGGCCTTAA

Protein sequence

MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLAGERLGLGP
BLAST of Cp4.1LG13g10950 vs. Swiss-Prot
Match: PCS1L_ARATH (Aspartic proteinase PCS1 OS=Arabidopsis thaliana GN=PCS1 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 4.8e-150
Identity = 258/420 (61.43%), Postives = 320/420 (76.19%), Query Frame = 1

Query: 16  SFKQGLCFSATQTMVLPLKTQMGVTS-RPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDT 75
           SF      S++QT+VLPLKT++  T  RP++KL FHHNVTLTV+LT+G+PPQ ++MV+DT
Sbjct: 33  SFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDT 92

Query: 76  GSELSWLHCKKTPNLNSV--FNPLSSSSYSPVPCASPVCRTRTRDLPNPVTCDPKKLCHV 135
           GSELSWL C ++ N N V  F+P  SSSYSP+PC+SP CRTRTRD   P +CD  KLCH 
Sbjct: 93  GSELSWLRCNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHA 152

Query: 136 FVSYADASSLEGNLASDTFRVGSSAQPGTF-FGCMDSGFSSNSEEDAKTTGLMGMNRGSL 195
            +SYADASS EGNLA++ F  G+S       FGCM S   S+ EED KTTGL+GMNRGSL
Sbjct: 153 TLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSL 212

Query: 196 SFVTQLGLPKFSYCISGRDS-SGVLLFGDVSLSWLGNLTYTPLVQMSTPLPYYDRVAYTV 255
           SF++Q+G PKFSYCISG D   G LL GD + +WL  L YTPL+++STPLPY+DRVAYTV
Sbjct: 213 SFISQMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTV 272

Query: 256 QLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGIL 315
           QL GI+V  K+L +PKS+  PDHTGAGQTMVDSGTQFTFLLGPVYTAL++ FL +T GIL
Sbjct: 273 QLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGIL 332

Query: 316 VPLGDPNFVFQGAMDLCYSVPE---KQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMV 375
               DP+FVFQG MDLCY +     + G L  LP VSL+F GAE+ V G+ L+Y+VP + 
Sbjct: 333 TVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLT 392

Query: 376 RGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLAGERLGLG 428
            G D V+C TFGNSDL+G+EA+VIGHHHQQN+W+EFDL +SR+G     CD++G+RLG+G
Sbjct: 393 VGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

BLAST of Cp4.1LG13g10950 vs. Swiss-Prot
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.3e-38
Identity = 114/367 (31.06%), Postives = 172/367 (46.87%), Query Frame = 1

Query: 57  VSLTLGSPPQPVTMVLDTGSELSWLHCKKTPNLNS----VFNPLSSSSYSPVPCASPVCR 116
           +++ +G+P    + ++DTGS+L W  C+      S    +FNP  SSS+S +PC S  C+
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQ 157

Query: 117 TRTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSS 176
               DLP+  TC+  + C     Y D S+ +G +A++TF   +S+ P   FGC   G  +
Sbjct: 158 ----DLPSE-TCNNNE-CQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGC---GEDN 217

Query: 177 NSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCIS--GRDSSGVLLFGDVSLSWLGNLTY 236
                    GL+GM  G LS  +QLG+ +FSYC++  G  S   L  G  +         
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS 277

Query: 237 TPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFL 296
           T L+  S    Y     Y + L GI VG   L +P S F     G G  ++DSGT  T+L
Sbjct: 278 TTLIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 337

Query: 297 LGPVYTALKNEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGA 356
               Y A+   F   T  I +P  D +      +  C+  P   G    +P +S+ F G 
Sbjct: 338 PQDAYNAVAQAF---TDQINLPTVDES---SSGLSTCFQQP-SDGSTVQVPEISMQFDGG 397

Query: 357 EMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRV 416
            + +G + +      ++   + V CL  G+S  LGI  F  G+  QQ   + +DL    V
Sbjct: 398 VLNLGEQNI------LISPAEGVICLAMGSSSQLGISIF--GNIQQQETQVLYDLQNLAV 435

Query: 417 GFVETRC 418
            FV T+C
Sbjct: 458 SFVPTQC 435

BLAST of Cp4.1LG13g10950 vs. Swiss-Prot
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 6.4e-38
Identity = 111/368 (30.16%), Postives = 182/368 (49.46%), Query Frame = 1

Query: 57  VSLTLGSPPQPVTMVLDTGSELSWLHCKKTPNL----NSVFNPLSSSSYSPVPCASPVCR 116
           ++L++G+P QP + ++DTGS+L W  C+           +FNP  SSS+S +PC+S +C+
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQ 156

Query: 117 TRTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSS 176
                L +P TC     C     Y D S  +G++ ++T   GS + P   FGC   G ++
Sbjct: 157 A----LSSP-TCS-NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC---GENN 216

Query: 177 NSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCIS--GRDSSGVLLFGDVSLSWLGNLTY 236
                    GL+GM RG LS  +QL + KFSYC++  G  +   LL G ++ S       
Sbjct: 217 QGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPN 276

Query: 237 TPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFA-PDHTGAGQTMVDSGTQFTF 296
           T L+Q S+ +P +    Y + L+G+ VG+  L +  S FA   + G G  ++DSGT  T+
Sbjct: 277 TTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTY 336

Query: 297 LLGPVYTALKNEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRG 356
            +   Y +++ EF+ Q   I +P+ + +       DLC+  P     L  +P   + F G
Sbjct: 337 FVNNAYQSVRQEFISQ---INLPVVNGS---SSGFDLCFQTPSDPSNL-QIPTFVMHFDG 396

Query: 357 AEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSR 416
            ++ +  E         +   + + CL  G+S   G+  F  G+  QQN+ + +D   S 
Sbjct: 397 GDLELPSENY------FISPSNGLICLAMGSSS-QGMSIF--GNIQQQNMLVVYDTGNSV 434

Query: 417 VGFVETRC 418
           V F   +C
Sbjct: 457 VSFASAQC 434

BLAST of Cp4.1LG13g10950 vs. Swiss-Prot
Match: APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.0e-32
Identity = 118/370 (31.89%), Postives = 173/370 (46.76%), Query Frame = 1

Query: 59  LTLGSPPQPVTMVLDTGSELSWLHCKKTPNLNS----VFNPLSSSSYSPVPCASPVCRTR 118
           L +G+P + V MVLDTGS++ WL C       S    +F+P  S +Y+ +PC+SP CR  
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRR- 205

Query: 119 TRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNS 178
              L +      +K C   VSY D S   G+ +++T     +   G   GC       N 
Sbjct: 206 ---LDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGC----GHDNE 265

Query: 179 EEDAKTTGLMGMNRGSLSFVTQLG---LPKFSYCISGRDSS---GVLLFGDVSLSWLGNL 238
                  GL+G+ +G LSF  Q G     KFSYC+  R +S     ++FG+ ++S +   
Sbjct: 266 GLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIAR- 325

Query: 239 TYTPLVQMSTPLPYYDRVAYTVQLDGIRV-GNKILALPKSIFAPDHTGAGQTMVDSGTQF 298
            +TPL+      P  D   Y V L GI V G ++  +  S+F  D  G G  ++DSGT  
Sbjct: 326 -FTPLLSN----PKLD-TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSV 385

Query: 299 TFLLGPVYTALKNEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMF 358
           T L+ P Y A+++ F V  K +      P+F      D C+ +         +P V L F
Sbjct: 386 TRLIRPAYIAMRDAFRVGAKTL---KRAPDF---SLFDTCFDLSNMNE--VKVPTVVLHF 445

Query: 359 RGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAK 418
           RGA+  V      Y +P    G     C  F  + + G+   +IG+  QQ   + +DLA 
Sbjct: 446 RGAD--VSLPATNYLIPVDTNG---KFCFAFAGT-MGGLS--IIGNIQQQGFRVVYDLAS 484

BLAST of Cp4.1LG13g10950 vs. Swiss-Prot
Match: AP25_ORYSJ (Aspartyl protease 25 OS=Oryza sativa subsp. japonica GN=AP25 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.8e-30
Identity = 123/378 (32.54%), Postives = 181/378 (47.88%), Query Frame = 1

Query: 61  LGSPPQPVTMVLDTGSELSWLHCKK--TPNLNSVFNPLSSSSYSPVPCASPVCRT-RTRD 120
           LGSP Q + + LDT ++ +W HC    T   +S+F P +SSSY+ +PC+S  C   + + 
Sbjct: 85  LGSPSQQLLLALDTSADATWAHCSPCGTCPSSSLFAPANSSSYASLPCSSSWCPLFQGQA 144

Query: 121 LPNP-----VTCDPKKL--CHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDS-- 180
            P P         P  L  C     +ADAS  +  LASDT R+G  A P   FGC+ S  
Sbjct: 145 CPAPQGGGDAAPPPATLPTCAFSKPFADAS-FQAALASDTLRLGKDAIPNYTFGCVSSVT 204

Query: 181 GFSSNSEEDAKTTGLMGMNRGSLSFVTQLGL---PKFSYCISGRDS---SGVLLFGDVSL 240
           G ++N        GL+G+ RG ++ ++Q G      FSYC+    S   SG L  G    
Sbjct: 205 GPTTNMPRQ----GLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFSGSLRLG-AGG 264

Query: 241 SWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDH-TGAGQTMV 300
               ++ YTP+++     P+   + Y V + G+ VG+  + +P   FA D  TGAG T+V
Sbjct: 265 GQPRSVRYTPMLRN----PHRSSLYY-VNVTGLSVGHAWVKVPAGSFAFDAATGAG-TVV 324

Query: 301 DSGTQFTFLLGPVYTALKNEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLP 360
           DSGT  T    PVY AL+ EF  Q   +  P G   +   GA D C++  E      P  
Sbjct: 325 DSGTVITRWTAPVYAALREEFRRQ---VAAPSG---YTSLGAFDTCFNTDEVAAGGAPAV 384

Query: 361 VVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNS-DLLGIEAFVIGHHHQQNVW 419
            V  M  G ++ +  E  +     +      + CL    +   +     VI +  QQN+ 
Sbjct: 385 TVH-MDGGVDLALPMENTL-----IHSSATPLACLAMAEAPQNVNSVVNVIANLQQQNIR 438

BLAST of Cp4.1LG13g10950 vs. TrEMBL
Match: E5GC71_CUCME (Aspartic proteinase nepenthesin-1 OS=Cucumis melo subsp. melo PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 8.6e-215
Identity = 368/411 (89.54%), Postives = 386/411 (93.92%), Query Frame = 1

Query: 18  KQGLCFSATQT-MVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDTGS 77
           KQ LCFSAT T MVLPL+TQMG+ S+PSNKLSFHHNVTLTVSLT+GSPPQ VTMVLDTGS
Sbjct: 2   KQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS 61

Query: 78  ELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPVTCDPKKLCHVFVSY 137
           ELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SPVCRTRTRDLPNPVTCDPKKLCH  VSY
Sbjct: 62  ELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKKLCHAIVSY 121

Query: 138 ADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 197
           ADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ
Sbjct: 122 ADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 181

Query: 198 LGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIR 257
           LGLPKFSYCISGRDSSGVLLFGD  LSWLGNLTYTPLVQ+STPLPY+DRVAYTVQLDGIR
Sbjct: 182 LGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPLPYFDRVAYTVQLDGIR 241

Query: 258 VGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGILVPLGDP 317
           VGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+NEFL QTKG+L PLGDP
Sbjct: 242 VGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRNEFLEQTKGVLAPLGDP 301

Query: 318 NFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCL 377
           NFVFQGAMDLCY VP   GKLP LP VSLMFRGAEMVVGGEVL+YKVPGM++G + V+CL
Sbjct: 302 NFVFQGAMDLCYRVP-AGGKLPELPAVSLMFRGAEMVVGGEVLLYKVPGMMKGKEWVYCL 361

Query: 378 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLAGERLGLG 428
           TFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGFVETRCDLAG+RLGLG
Sbjct: 362 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLG 411

BLAST of Cp4.1LG13g10950 vs. TrEMBL
Match: A0A0A0K8C7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G008640 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 4.3e-214
Identity = 370/428 (86.45%), Postives = 393/428 (91.82%), Query Frame = 1

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQT-MVLPLKTQMGVTSRPSNKLSFHHNVTLTVSL 60
           MAFF      L+ C+S KQ LCFSAT T MVLPL TQMG+ S+PSNKLSFHHNVTLTVSL
Sbjct: 1   MAFFFFFF--LLVCLSLKQSLCFSATPTSMVLPLNTQMGLISQPSNKLSFHHNVTLTVSL 60

Query: 61  TLGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLP 120
           T+GSPPQ VTMVLDTGSELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SP+CRTRTRDLP
Sbjct: 61  TVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLP 120

Query: 121 NPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAK 180
           NPVTCDPKKLCH  VSYADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAK
Sbjct: 121 NPVTCDPKKLCHAIVSYADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGD+ LSWLGNLTYTPLVQ+STP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDLHLSWLGNLTYTPLVQISTP 240

Query: 241 LPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPY+DRVAYTVQLDGIRVGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+
Sbjct: 241 LPYFDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALR 300

Query: 301 NEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVL 360
           NEFL QTKG+L PLGDPNFVFQGAMDLCYSV    GKLP LP VSLMFRGAEMVVGGEVL
Sbjct: 301 NEFLEQTKGVLAPLGDPNFVFQGAMDLCYSV-AAGGKLPTLPSVSLMFRGAEMVVGGEVL 360

Query: 361 MYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDL 420
           +Y+VP M++G + V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGFVETRCDL
Sbjct: 361 LYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDL 420

Query: 421 AGERLGLG 428
           A +RLG+G
Sbjct: 421 ASQRLGVG 425

BLAST of Cp4.1LG13g10950 vs. TrEMBL
Match: M5XJM3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006030mg PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 4.4e-187
Identity = 321/423 (75.89%), Postives = 357/423 (84.40%), Query Frame = 1

Query: 10  LLICCVSFKQGLCFSATQ--TMVLPLKTQM---GVTSRPSNKLSFHHNVTLTVSLTLGSP 69
           LL+ C      LCFSA++  T++LPLKT+    G     +NKLSFHHNVTLT+ L++GSP
Sbjct: 8   LLLLCFFTHCTLCFSASKPRTLILPLKTETLPHGFAPHSTNKLSFHHNVTLTIQLSVGSP 67

Query: 70  PQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPVTC 129
           PQ VTMVLDTGSELSWLHCKK PNLNSVFNPL+S SYS +PC+SPVCRTRTRD   PV+C
Sbjct: 68  PQNVTMVLDTGSELSWLHCKKAPNLNSVFNPLASKSYSTIPCSSPVCRTRTRDFSIPVSC 127

Query: 130 DPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLM 189
           DPKKLCH  +SYADASS+EGNLAS+TF +G S QPGT FGCMDSGFSSNSEEDAKTTGLM
Sbjct: 128 DPKKLCHATLSYADASSIEGNLASETFVLGLSTQPGTIFGCMDSGFSSNSEEDAKTTGLM 187

Query: 190 GMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPLPYYD 249
           GMNRGSLSFVTQ+G PKFSYCISGRDSSG+LLFG+   +WL  L YTPLVQ+STPLPY+D
Sbjct: 188 GMNRGSLSFVTQMGFPKFSYCISGRDSSGILLFGEAKFAWLRPLNYTPLVQISTPLPYFD 247

Query: 250 RVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLV 309
           RVAYTVQL+GIRV  K+L LPKS+F PDHTGAGQTMVDSGTQFTFLLGPVYTALKNEF+ 
Sbjct: 248 RVAYTVQLEGIRVSGKVLPLPKSVFVPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFIQ 307

Query: 310 QTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVP 369
           QTK +L  L DPNFVFQGAMDLCY VP  +  LP LP V+LMF+GAEM V GE L+Y+VP
Sbjct: 308 QTKPVLRVLNDPNFVFQGAMDLCYQVPMNRPSLPQLPTVTLMFQGAEMSVSGERLLYRVP 367

Query: 370 GMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLAGERL 428
           GMVRG D V+C TFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVG  E RCDLA +RL
Sbjct: 368 GMVRGSDSVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLEKSRVGVAEVRCDLASQRL 427

BLAST of Cp4.1LG13g10950 vs. TrEMBL
Match: A5BM56_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_016757 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 7.1e-185
Identity = 319/430 (74.19%), Postives = 364/430 (84.65%), Query Frame = 1

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQM---GVTSRPSNKLSFHHNVTLTV 60
           M  FL LL L    +SF   LC ++T  ++LPLKTQ+   G   RPS+KLSFHHNV+LTV
Sbjct: 1   MLSFLLLLLLSQLFLSFT--LCLASTPAVILPLKTQVLPSGSVPRPSSKLSFHHNVSLTV 60

Query: 61  SLTLGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRD 120
           SLT+GSPPQ VTMVLDTGSELSWLHCKK PNL+SVF+PL SSSYSP+PC SP CRTRTRD
Sbjct: 61  SLTVGSPPQTVTMVLDTGSELSWLHCKKAPNLHSVFDPLRSSSYSPIPCTSPTCRTRTRD 120

Query: 121 LPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEED 180
              PV+CD KKLCH  +SYADASS+EGNLASDTF +G+SA P T FGCMDSGFSSNS+ED
Sbjct: 121 FSIPVSCDKKKLCHAIISYADASSIEGNLASDTFHIGNSAIPATIFGCMDSGFSSNSDED 180

Query: 181 AKTTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMS 240
           +KTTGL+GMNRGSLSFVTQ+GL KFSYCISG+DSSG+LLFG+ S SWL  L YTPLVQ+S
Sbjct: 181 SKTTGLIGMNRGSLSFVTQMGLQKFSYCISGQDSSGILLFGESSFSWLKALKYTPLVQIS 240

Query: 241 TPLPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTA 300
           TPLPY+DRVAYTVQL+GI+V N +L LPKS++APDHTGAGQTMVDSGTQFTFLLGPVYTA
Sbjct: 241 TPLPYFDRVAYTVQLEGIKVANSMLQLPKSVYAPDHTGAGQTMVDSGTQFTFLLGPVYTA 300

Query: 301 LKNEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGE 360
           LKNEF+ QTK  L  L DPNFVFQGAMDLCY VP  +  LPPLP V+LMFRGAEM V  E
Sbjct: 301 LKNEFVRQTKASLKVLEDPNFVFQGAMDLCYRVPLTRRTLPPLPTVTLMFRGAEMSVSAE 360

Query: 361 VLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRC 420
            LMY+VPG++RG D V+C TFGNS+LLG+E+++IGHHHQQNVWMEFDLAKSRVGF E RC
Sbjct: 361 RLMYRVPGVIRGSDSVYCFTFGNSELLGVESYIIGHHHQQNVWMEFDLAKSRVGFAEVRC 420

Query: 421 DLAGERLGLG 428
            LAG+RLG+G
Sbjct: 421 XLAGQRLGVG 428

BLAST of Cp4.1LG13g10950 vs. TrEMBL
Match: F6HNV7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g01980 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 2.1e-184
Identity = 310/407 (76.17%), Postives = 354/407 (86.98%), Query Frame = 1

Query: 24  SATQTMVLPLKTQM---GVTSRPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDTGSELSW 83
           ++T  ++LPLKTQ+   G   RPS+KLSFHHNV+LTVSLT+GSPPQ VTMVLDTGSELSW
Sbjct: 356 TSTPAVILPLKTQVLPSGSVPRPSSKLSFHHNVSLTVSLTVGSPPQTVTMVLDTGSELSW 415

Query: 84  LHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPVTCDPKKLCHVFVSYADAS 143
           LHCKK PNL+SVF+PL SSSYSP+PC SP CRTRTRD   PV+CD KKLCH  +SYADAS
Sbjct: 416 LHCKKAPNLHSVFDPLRSSSYSPIPCTSPTCRTRTRDFSIPVSCDKKKLCHAIISYADAS 475

Query: 144 SLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLGLP 203
           S+EGNLASDTF +G+SA P T FGCMDSGFSSNS+ED+KTTGL+GMNRGSLSFVTQ+GL 
Sbjct: 476 SIEGNLASDTFHIGNSAIPATIFGCMDSGFSSNSDEDSKTTGLIGMNRGSLSFVTQMGLQ 535

Query: 204 KFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNK 263
           KFSYCISG+DSSG+LLFG+ S SWL  L YTPLVQ+STPLPY+DRVAYTVQL+GI+V N 
Sbjct: 536 KFSYCISGQDSSGILLFGESSFSWLKALKYTPLVQISTPLPYFDRVAYTVQLEGIKVANS 595

Query: 264 ILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGILVPLGDPNFVF 323
           +L LPKS++APDHTGAGQTMVDSGTQFTFLLGPVYTALKNEF+ QTK  L  L DPNFVF
Sbjct: 596 MLQLPKSVYAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFVRQTKASLKVLEDPNFVF 655

Query: 324 QGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGN 383
           QGAMDLCY VP  +  LPPLP V+LMFRGAEM V  E LMY+VPG++RG D V+C TFGN
Sbjct: 656 QGAMDLCYRVPLTRRTLPPLPTVTLMFRGAEMSVSAERLMYRVPGVIRGSDSVYCFTFGN 715

Query: 384 SDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLAGERLGLG 428
           S+LLG+E+++IGHHHQQNVWMEFDLAKSRVGF E RCDLAG+RLG+G
Sbjct: 716 SELLGVESYIIGHHHQQNVWMEFDLAKSRVGFAEVRCDLAGQRLGVG 762

BLAST of Cp4.1LG13g10950 vs. TAIR10
Match: AT2G39710.1 (AT2G39710.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 598.2 bits (1541), Expect = 4.0e-171
Identity = 303/429 (70.63%), Postives = 346/429 (80.65%), Query Frame = 1

Query: 4   FLRLLQLLICCVSFKQGLC--FSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTL 63
           FLR+  LL+    F    C   S  QT++  LKTQ  +    S+KLSF HNVTLTV+L +
Sbjct: 16  FLRISVLLLI---FPLTFCKTSSTNQTLLFSLKTQK-LPQSSSDKLSFRHNVTLTVTLAV 75

Query: 64  GSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNP 123
           G PPQ ++MVLDTGSELSWLHCKK+PNL SVFNP+SSS+YSPVPC+SP+CRTRTRDLP P
Sbjct: 76  GDPPQNISMVLDTGSELSWLHCKKSPNLGSVFNPVSSSTYSPVPCSSPICRTRTRDLPIP 135

Query: 124 VTCDPKK-LCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 183
            +CDPK  LCHV +SYADA+S+EGNLA +TF +GS  +PGT FGCMDSG SSNSEEDAK+
Sbjct: 136 ASCDPKTHLCHVAISYADATSIEGNLAHETFVIGSVTRPGTLFGCMDSGLSSNSEEDAKS 195

Query: 184 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPL 243
           TGLMGMNRGSLSFV QLG  KFSYCISG DSSG LL GD S SWLG + YTPLV  STPL
Sbjct: 196 TGLMGMNRGSLSFVNQLGFSKFSYCISGSDSSGFLLLGDASYSWLGPIQYTPLVLQSTPL 255

Query: 244 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 303
           PY+DRVAYTVQL+GIRVG+KIL+LPKS+F PDHTGAGQTMVDSGTQFTFL+GPVYTALKN
Sbjct: 256 PYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKN 315

Query: 304 EFLVQTKGILVPLGDPNFVFQGAMDLCYSV-PEKQGKLPPLPVVSLMFRGAEMVVGGEVL 363
           EF+ QTK +L  + DP+FVFQG MDLCY V    +     LP+VSLMFRGAEM V G+ L
Sbjct: 316 EFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAEMSVSGQKL 375

Query: 364 MYKVPGM-VRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFV-ETRC 423
           +Y+V G    G ++V+C TFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGF    RC
Sbjct: 376 LYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAGNVRC 435

Query: 424 DLAGERLGL 427
           DLA +RLGL
Sbjct: 436 DLASQRLGL 440

BLAST of Cp4.1LG13g10950 vs. TAIR10
Match: AT5G02190.1 (AT5G02190.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 532.3 bits (1370), Expect = 2.7e-151
Identity = 258/420 (61.43%), Postives = 320/420 (76.19%), Query Frame = 1

Query: 16  SFKQGLCFSATQTMVLPLKTQMGVTS-RPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDT 75
           SF      S++QT+VLPLKT++  T  RP++KL FHHNVTLTV+LT+G+PPQ ++MV+DT
Sbjct: 33  SFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDT 92

Query: 76  GSELSWLHCKKTPNLNSV--FNPLSSSSYSPVPCASPVCRTRTRDLPNPVTCDPKKLCHV 135
           GSELSWL C ++ N N V  F+P  SSSYSP+PC+SP CRTRTRD   P +CD  KLCH 
Sbjct: 93  GSELSWLRCNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHA 152

Query: 136 FVSYADASSLEGNLASDTFRVGSSAQPGTF-FGCMDSGFSSNSEEDAKTTGLMGMNRGSL 195
            +SYADASS EGNLA++ F  G+S       FGCM S   S+ EED KTTGL+GMNRGSL
Sbjct: 153 TLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSL 212

Query: 196 SFVTQLGLPKFSYCISGRDS-SGVLLFGDVSLSWLGNLTYTPLVQMSTPLPYYDRVAYTV 255
           SF++Q+G PKFSYCISG D   G LL GD + +WL  L YTPL+++STPLPY+DRVAYTV
Sbjct: 213 SFISQMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTV 272

Query: 256 QLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGIL 315
           QL GI+V  K+L +PKS+  PDHTGAGQTMVDSGTQFTFLLGPVYTAL++ FL +T GIL
Sbjct: 273 QLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGIL 332

Query: 316 VPLGDPNFVFQGAMDLCYSVPE---KQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMV 375
               DP+FVFQG MDLCY +     + G L  LP VSL+F GAE+ V G+ L+Y+VP + 
Sbjct: 333 TVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLT 392

Query: 376 RGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLAGERLGLG 428
            G D V+C TFGNSDL+G+EA+VIGHHHQQN+W+EFDL +SR+G     CD++G+RLG+G
Sbjct: 393 VGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

BLAST of Cp4.1LG13g10950 vs. TAIR10
Match: AT5G37540.1 (AT5G37540.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 243.0 bits (619), Expect = 3.3e-64
Identity = 150/402 (37.31%), Postives = 215/402 (53.48%), Query Frame = 1

Query: 41  SRPSNKLSFHHNV----TLTVSLTLGSPPQPVTMVLDTGSELSWLHC------KKTPNLN 100
           S PS+  +F  N+     L +SL +G+P Q   +VLDTGS+LSW+ C      K  P   
Sbjct: 62  SPPSSPYTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPT 121

Query: 101 SVFNPLSSSSYSPVPCASPVCRTRTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDT 160
           + F+P  SSS+S +PC+ P+C+ R  D   P +CD  +LCH    YAD +  EGNL  + 
Sbjct: 122 TSFDPSLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEK 181

Query: 161 FRVGSS-AQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCISGR 220
           F   +S   P    GC        ++E     G++GMN G LSF++Q  + KFSYCI  R
Sbjct: 182 FTFSNSQTTPPLILGC--------AKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTR 241

Query: 221 D------SSGVLLFGDVSLS----WLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGN 280
                  S+G    GD   S    ++  LT+      S  +P  D +AYTV L GIR+G 
Sbjct: 242 SNRPGLASTGSFYLGDNPNSRGFKYVSLLTFP----QSQRMPNLDPLAYTVPLQGIRIGQ 301

Query: 281 KILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGILVPLGDPNFV 340
           K L +P S+F PD  G+GQTMVDSG++FT L+   Y  +K E +V+  G  +  G   +V
Sbjct: 302 KRLNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEE-IVRLVGSRLKKG---YV 361

Query: 341 FQGAMDLCYSVPEKQ--GKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLT 400
           +    D+C+        G+L    +  L+F   E   G E+L+ K   +V  G  +HC+ 
Sbjct: 362 YGSTADMCFDGNHSMEIGRL----IGDLVF---EFGRGVEILVEKQSLLVNVGGGIHCVG 421

Query: 401 FGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDL 420
            G S +LG  + +IG+ HQQN+W+EFD+   RVGF +  C L
Sbjct: 422 IGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAECRL 440

BLAST of Cp4.1LG13g10950 vs. TAIR10
Match: AT1G66180.1 (AT1G66180.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 238.0 bits (606), Expect = 1.0e-62
Identity = 145/396 (36.62%), Postives = 210/396 (53.03%), Query Frame = 1

Query: 40  TSRPSNKLS-FHHNVTLTVSLTLGSPPQPVTMVLDTGSELSWLHC---KKTPNLNSVFNP 99
           +S P N  S F +++ L +SL +G+PPQ   MVLDTGS+LSW+ C   K  P   + F+P
Sbjct: 56  SSPPYNFRSRFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTSFDP 115

Query: 100 LSSSSYSPVPCASPVCRTRTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGS 159
             SSS+S +PC+ P+C+ R  D   P +CD  +LCH    YAD +  EGNL  +     +
Sbjct: 116 SLSSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSN 175

Query: 160 SA-QPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCISGRDS--- 219
           +   P    GC        + E +   G++GMNRG LSFV+Q  + KFSYCI  + +   
Sbjct: 176 TEITPPLILGC--------ATESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPG 235

Query: 220 ---SGVLLFGDVSLSWLGNLTYTPLVQM--STPLPYYDRVAYTVQLDGIRVGNKILALPK 279
              +G    GD   S      Y  L+    S  +P  D +AYTV + GIR G K L +  
Sbjct: 236 FTPTGSFYLGDNPNSH--GFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISG 295

Query: 280 SIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGILVPLGDPNFVFQGAMDL 339
           S+F PD  G+GQTMVDSG++FT L+   Y  ++ E + +    L       +V+ G  D+
Sbjct: 296 SVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRL----KKGYVYGGTADM 355

Query: 340 CYS-----VPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNS 399
           C+      +P   G L     V +  RG E++V  E ++  V      G  +HC+  G S
Sbjct: 356 CFDGNVAMIPRLIGDL-----VFVFTRGVEILVPKERVLVNV------GGGIHCVGIGRS 415

Query: 400 DLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRC 418
            +LG  + +IG+ HQQN+W+EFD+   RVGF +  C
Sbjct: 416 SMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

BLAST of Cp4.1LG13g10950 vs. TAIR10
Match: AT2G03200.1 (AT2G03200.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-38
Identity = 120/377 (31.83%), Postives = 182/377 (48.28%), Query Frame = 1

Query: 57  VSLTLGSPPQPVTMVLDTGSELSWLHCKKTPNL----NSVFNPLSSSSYSPVPCASPVCR 116
           + L++G+P    + ++DTGS+L W  CK           +F+P  SSSYS V C+S +C 
Sbjct: 109 MELSIGNPAVKYSAIVDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCN 168

Query: 117 TRTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRV-GSSAQPGTFFGCMDSGFS 176
                LP     + K  C    +Y D SS  G LA++TF     ++  G  FGC   G  
Sbjct: 169 A----LPRSNCNEDKDACEYLYTYGDYSSTRGLLATETFTFEDENSISGIGFGC---GVE 228

Query: 177 SNSEEDAKTTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSS--------GVLLFGDVS-- 236
           +  +  ++ +GL+G+ RG LS ++QL   KFSYC++  + S        G L  G V+  
Sbjct: 229 NEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKT 288

Query: 237 -LSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTM 296
             S  G +T T  +  +   P +    Y ++L GI VG K L++ KS F     G G  +
Sbjct: 289 GASLDGEVTKTMSLLRNPDQPSF----YYLELQGITVGAKRLSVEKSTFELAEDGTGGMI 348

Query: 297 VDSGTQFTFLLGPVYTALKNEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPL 356
           +DSGT  T+L    +  LK EF   T  + +P+ D        +DLC+ +P+   K   +
Sbjct: 349 IDSGTTITYLEETAFKVLKEEF---TSRMSLPVDDSG---STGLDLCFKLPD-AAKNIAV 408

Query: 357 PVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVW 416
           P +   F+GA++ + GE  M     +      V CL  G+S+ + I     G+  QQN  
Sbjct: 409 PKMIFHFKGADLELPGENYM-----VADSSTGVLCLAMGSSNGMSI----FGNVQQQNFN 458

Query: 417 MEFDLAKSRVGFVETRC 418
           +  DL K  V FV T C
Sbjct: 469 VLHDLEKETVSFVPTEC 458

BLAST of Cp4.1LG13g10950 vs. NCBI nr
Match: gi|659081534|ref|XP_008441381.1| (PREDICTED: aspartic proteinase PCS1 [Cucumis melo])

HSP 1 Score: 755.4 bits (1949), Expect = 5.5e-215
Identity = 373/428 (87.15%), Postives = 394/428 (92.06%), Query Frame = 1

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQT-MVLPLKTQMGVTSRPSNKLSFHHNVTLTVSL 60
           M+FF      L+  +S KQ LCFSAT T MVLPL+TQMG+ S+PSNKLSFHHNVTLTVSL
Sbjct: 1   MSFFFFFF-FLVVSLSMKQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSL 60

Query: 61  TLGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLP 120
           T+GSPPQ VTMVLDTGSELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SPVCRTRTRDLP
Sbjct: 61  TVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLP 120

Query: 121 NPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAK 180
           NPVTCDPKKLCH  VSYADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAK
Sbjct: 121 NPVTCDPKKLCHAIVSYADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGD  LSWLGNLTYTPLVQ+STP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTP 240

Query: 241 LPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPY+DRVAYTVQLDGIRVGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+
Sbjct: 241 LPYFDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALR 300

Query: 301 NEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVL 360
           NEFL QTKG+L PLGDPNFVFQGAMDLCY VP   GKLP LP VSLMFRGAEMVVGGEVL
Sbjct: 301 NEFLEQTKGVLAPLGDPNFVFQGAMDLCYRVP-AGGKLPELPAVSLMFRGAEMVVGGEVL 360

Query: 361 MYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDL 420
           +YKVPGM++G + V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGFVETRCDL
Sbjct: 361 LYKVPGMMKGKEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDL 420

Query: 421 AGERLGLG 428
           AG+RLGLG
Sbjct: 421 AGQRLGLG 426

BLAST of Cp4.1LG13g10950 vs. NCBI nr
Match: gi|307136234|gb|ADN34070.1| (aspartic proteinase nepenthesin-1 precursor [Cucumis melo subsp. melo])

HSP 1 Score: 754.2 bits (1946), Expect = 1.2e-214
Identity = 368/411 (89.54%), Postives = 386/411 (93.92%), Query Frame = 1

Query: 18  KQGLCFSATQT-MVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDTGS 77
           KQ LCFSAT T MVLPL+TQMG+ S+PSNKLSFHHNVTLTVSLT+GSPPQ VTMVLDTGS
Sbjct: 2   KQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS 61

Query: 78  ELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPVTCDPKKLCHVFVSY 137
           ELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SPVCRTRTRDLPNPVTCDPKKLCH  VSY
Sbjct: 62  ELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKKLCHAIVSY 121

Query: 138 ADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 197
           ADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ
Sbjct: 122 ADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 181

Query: 198 LGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIR 257
           LGLPKFSYCISGRDSSGVLLFGD  LSWLGNLTYTPLVQ+STPLPY+DRVAYTVQLDGIR
Sbjct: 182 LGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPLPYFDRVAYTVQLDGIR 241

Query: 258 VGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGILVPLGDP 317
           VGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+NEFL QTKG+L PLGDP
Sbjct: 242 VGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRNEFLEQTKGVLAPLGDP 301

Query: 318 NFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCL 377
           NFVFQGAMDLCY VP   GKLP LP VSLMFRGAEMVVGGEVL+YKVPGM++G + V+CL
Sbjct: 302 NFVFQGAMDLCYRVP-AGGKLPELPAVSLMFRGAEMVVGGEVLLYKVPGMMKGKEWVYCL 361

Query: 378 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLAGERLGLG 428
           TFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGFVETRCDLAG+RLGLG
Sbjct: 362 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLG 411

BLAST of Cp4.1LG13g10950 vs. NCBI nr
Match: gi|778709340|ref|XP_011656388.1| (PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus])

HSP 1 Score: 751.9 bits (1940), Expect = 6.1e-214
Identity = 370/428 (86.45%), Postives = 393/428 (91.82%), Query Frame = 1

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQT-MVLPLKTQMGVTSRPSNKLSFHHNVTLTVSL 60
           MAFF      L+ C+S KQ LCFSAT T MVLPL TQMG+ S+PSNKLSFHHNVTLTVSL
Sbjct: 1   MAFFFFFF--LLVCLSLKQSLCFSATPTSMVLPLNTQMGLISQPSNKLSFHHNVTLTVSL 60

Query: 61  TLGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLP 120
           T+GSPPQ VTMVLDTGSELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SP+CRTRTRDLP
Sbjct: 61  TVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLP 120

Query: 121 NPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAK 180
           NPVTCDPKKLCH  VSYADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAK
Sbjct: 121 NPVTCDPKKLCHAIVSYADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGD+ LSWLGNLTYTPLVQ+STP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDLHLSWLGNLTYTPLVQISTP 240

Query: 241 LPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPY+DRVAYTVQLDGIRVGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+
Sbjct: 241 LPYFDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALR 300

Query: 301 NEFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVL 360
           NEFL QTKG+L PLGDPNFVFQGAMDLCYSV    GKLP LP VSLMFRGAEMVVGGEVL
Sbjct: 301 NEFLEQTKGVLAPLGDPNFVFQGAMDLCYSV-AAGGKLPTLPSVSLMFRGAEMVVGGEVL 360

Query: 361 MYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDL 420
           +Y+VP M++G + V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGFVETRCDL
Sbjct: 361 LYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDL 420

Query: 421 AGERLGLG 428
           A +RLG+G
Sbjct: 421 ASQRLGVG 425

BLAST of Cp4.1LG13g10950 vs. NCBI nr
Match: gi|645255765|ref|XP_008233645.1| (PREDICTED: aspartic proteinase PCS1 [Prunus mume])

HSP 1 Score: 671.0 bits (1730), Expect = 1.4e-189
Identity = 326/423 (77.07%), Postives = 359/423 (84.87%), Query Frame = 1

Query: 10  LLICCVSFKQGLCFSA--TQTMVLPLKTQM---GVTSRPSNKLSFHHNVTLTVSLTLGSP 69
           LL+ C      LCFSA  TQT++LPLKT+    G     +NKLSFHHNVTLT+ L++GSP
Sbjct: 7   LLLLCFFTHCTLCFSASKTQTLILPLKTETLPHGFAPHSTNKLSFHHNVTLTIQLSVGSP 66

Query: 70  PQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPVTC 129
           PQ VTMVLDTGSELSWLHCKK PNLNSVFNPL+S SYSP+PC+SPVCRTRTRD   PV+C
Sbjct: 67  PQKVTMVLDTGSELSWLHCKKAPNLNSVFNPLASKSYSPIPCSSPVCRTRTRDFSIPVSC 126

Query: 130 DPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLM 189
           DPKKLCH  +SYADASS+EGNLAS+TF +G S QPGT FGCMDSGFSSNSEEDAKTTGLM
Sbjct: 127 DPKKLCHATLSYADASSIEGNLASETFGLGLSTQPGTIFGCMDSGFSSNSEEDAKTTGLM 186

Query: 190 GMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPLPYYD 249
           GMNRGSLSFVTQ+GLPKFSYCISGRDSSGVLLFG+   +WL  L YTPLVQ+STPLPY+D
Sbjct: 187 GMNRGSLSFVTQMGLPKFSYCISGRDSSGVLLFGEAKFAWLRPLNYTPLVQISTPLPYFD 246

Query: 250 RVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLV 309
           RVAYTVQL+GIRV  K+L LPKS+F PDHTGAGQTMVDSGTQFTFLLGPVYTALKNEF+ 
Sbjct: 247 RVAYTVQLEGIRVSGKVLPLPKSVFVPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFIQ 306

Query: 310 QTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVP 369
           QTK +L  L DPNFVFQGAMDLCY VP  +  LP LP V+LMF+GAEM V GE L+Y+VP
Sbjct: 307 QTKPVLRVLNDPNFVFQGAMDLCYQVPMNRQSLPQLPTVTLMFQGAEMSVSGERLLYRVP 366

Query: 370 GMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLAGERL 428
           GMVRG D V+C TFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVG  E RCDLA +RL
Sbjct: 367 GMVRGSDSVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLEKSRVGVAEVRCDLASQRL 426

BLAST of Cp4.1LG13g10950 vs. NCBI nr
Match: gi|595999949|ref|XP_007218036.1| (hypothetical protein PRUPE_ppa006030mg [Prunus persica])

HSP 1 Score: 662.1 bits (1707), Expect = 6.4e-187
Identity = 321/423 (75.89%), Postives = 357/423 (84.40%), Query Frame = 1

Query: 10  LLICCVSFKQGLCFSATQ--TMVLPLKTQM---GVTSRPSNKLSFHHNVTLTVSLTLGSP 69
           LL+ C      LCFSA++  T++LPLKT+    G     +NKLSFHHNVTLT+ L++GSP
Sbjct: 8   LLLLCFFTHCTLCFSASKPRTLILPLKTETLPHGFAPHSTNKLSFHHNVTLTIQLSVGSP 67

Query: 70  PQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPVTC 129
           PQ VTMVLDTGSELSWLHCKK PNLNSVFNPL+S SYS +PC+SPVCRTRTRD   PV+C
Sbjct: 68  PQNVTMVLDTGSELSWLHCKKAPNLNSVFNPLASKSYSTIPCSSPVCRTRTRDFSIPVSC 127

Query: 130 DPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLM 189
           DPKKLCH  +SYADASS+EGNLAS+TF +G S QPGT FGCMDSGFSSNSEEDAKTTGLM
Sbjct: 128 DPKKLCHATLSYADASSIEGNLASETFVLGLSTQPGTIFGCMDSGFSSNSEEDAKTTGLM 187

Query: 190 GMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPLPYYD 249
           GMNRGSLSFVTQ+G PKFSYCISGRDSSG+LLFG+   +WL  L YTPLVQ+STPLPY+D
Sbjct: 188 GMNRGSLSFVTQMGFPKFSYCISGRDSSGILLFGEAKFAWLRPLNYTPLVQISTPLPYFD 247

Query: 250 RVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLV 309
           RVAYTVQL+GIRV  K+L LPKS+F PDHTGAGQTMVDSGTQFTFLLGPVYTALKNEF+ 
Sbjct: 248 RVAYTVQLEGIRVSGKVLPLPKSVFVPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFIQ 307

Query: 310 QTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVP 369
           QTK +L  L DPNFVFQGAMDLCY VP  +  LP LP V+LMF+GAEM V GE L+Y+VP
Sbjct: 308 QTKPVLRVLNDPNFVFQGAMDLCYQVPMNRPSLPQLPTVTLMFQGAEMSVSGERLLYRVP 367

Query: 370 GMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLAGERL 428
           GMVRG D V+C TFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVG  E RCDLA +RL
Sbjct: 368 GMVRGSDSVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLEKSRVGVAEVRCDLASQRL 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PCS1L_ARATH4.8e-15061.43Aspartic proteinase PCS1 OS=Arabidopsis thaliana GN=PCS1 PE=2 SV=1[more]
NEP2_NEPGR1.3e-3831.06Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1[more]
NEP1_NEPGR6.4e-3830.16Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1[more]
APF2_ARATH4.0e-3231.89Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1[more]
AP25_ORYSJ3.8e-3032.54Aspartyl protease 25 OS=Oryza sativa subsp. japonica GN=AP25 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
E5GC71_CUCME8.6e-21589.54Aspartic proteinase nepenthesin-1 OS=Cucumis melo subsp. melo PE=3 SV=1[more]
A0A0A0K8C7_CUCSA4.3e-21486.45Uncharacterized protein OS=Cucumis sativus GN=Csa_6G008640 PE=3 SV=1[more]
M5XJM3_PRUPE4.4e-18775.89Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006030mg PE=3 SV=1[more]
A5BM56_VITVI7.1e-18574.19Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_016757 PE=3 SV=1[more]
F6HNV7_VITVI2.1e-18476.17Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g01980 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT2G39710.14.0e-17170.63 Eukaryotic aspartyl protease family protein[more]
AT5G02190.12.7e-15161.43 Eukaryotic aspartyl protease family protein[more]
AT5G37540.13.3e-6437.31 Eukaryotic aspartyl protease family protein[more]
AT1G66180.11.0e-6236.62 Eukaryotic aspartyl protease family protein[more]
AT2G03200.11.4e-3831.83 Eukaryotic aspartyl protease family protein[more]
Match NameE-valueIdentityDescription
gi|659081534|ref|XP_008441381.1|5.5e-21587.15PREDICTED: aspartic proteinase PCS1 [Cucumis melo][more]
gi|307136234|gb|ADN34070.1|1.2e-21489.54aspartic proteinase nepenthesin-1 precursor [Cucumis melo subsp. melo][more]
gi|778709340|ref|XP_011656388.1|6.1e-21486.45PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus][more]
gi|645255765|ref|XP_008233645.1|1.4e-18977.07PREDICTED: aspartic proteinase PCS1 [Prunus mume][more]
gi|595999949|ref|XP_007218036.1|6.4e-18775.89hypothetical protein PRUPE_ppa006030mg [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR021109Peptidase_aspartic_dom_sf
IPR001969Aspartic_peptidase_AS
IPR001461Aspartic_peptidase_A1
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0012501 programmed cell death
cellular_component GO:0005575 cellular_component
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g10950.1Cp4.1LG13g10950.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 61..81
score: 2.5E-5coord: 213..226
score: 2.5E-5coord: 279..290
score: 2.
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 3..425
score: 6.3E
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 70..81
scor
IPR021109Aspartic peptidase domainGENE3DG3DSA:2.40.70.10coord: 56..219
score: 2.0E-35coord: 226..418
score: 5.7
IPR021109Aspartic peptidase domainunknownSSF50630Acid proteasescoord: 55..417
score: 4.78
NoneNo IPR availablePANTHERPTHR13683:SF347CYSTEINE-RICH PEPTIDE FAMILY PROTEINcoord: 3..425
score: 6.3E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG13g10950Cp4.1LG01g25520Cucurbita pepo (Zucchini)cpecpeB199
Cp4.1LG13g10950Cp4.1LG03g02600Cucurbita pepo (Zucchini)cpecpeB216
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG13g10950Cucurbita pepo (Zucchini)cpecpeB153
Cp4.1LG13g10950Cucurbita maxima (Rimu)cmacpeB911
Cp4.1LG13g10950Cucumber (Gy14) v2cgybcpeB908
Cp4.1LG13g10950Silver-seed gourdcarcpeB0019
Cp4.1LG13g10950Silver-seed gourdcarcpeB0123