Cp4.1LG12g11020 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG12g11020
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Description3-methyl-2-oxobutanoate hydroxymethyltransferase
LocationCp4.1LG12 : 8937510 .. 8940417 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAAAAGTTAGTTATATTTTAATTTTCATTCATTTTTTAAATTTTTATTTTAAAGTTTTAAGGATATGCATGAATTTGACACGTGTTGTCGGCGGGAGAATTCCACGTTTGCAAACCTGCAACTAATTCGCAGGCAACAGAGCAGCAACCAACCGATTGTGAAAATCCAATTCATTCGAAGACAAATTTCTCCCCATCAATGGCGCTCTTCAACCTCGTCACCAAGGCCGCAGTTCGTTCCACATCGCAATCGCTGCTCAACACGCTTCGTTGCATGAGCAATGTCCCTGAAAACACGGTCTACGGCGGCCCGACATCGAACACTCCAAACCAGAGAGTCACTCTCACCAATCTGAAACAAAAGTACAAGAAAGGCGAACCGATCACCATGGTCACTGCTTACGATTACCCCTCCGGTGTCCATTTGGATATGGCCGGCATTGATATCGCTTTGGTTGGCGATTCGGCTTCCATGGTTGTACACGGCCACGACACCACGTTGCCTATTACCTTGGATGAAATGCTTGTTCATTGCAGGTCTGTGGCTCGTGGGGCTAGGAGGCCGCTTTTGATCGGCGACTTGCCCTTTGGTACCTACGAATCCAGCTCCGTTCAGGTTATTTTCCTTTTGCCTCTTAGGTGTTCGATGAAATGATAGCCATCTGGATTTATTCAAAATCTAATTTCCATGCTTAGAGATGAGCCGCACGGGAATCTAATAAATTCTTTTTTCTTTTTTCATGCTAGATTGTAAAGGGTAAAAGCAGTTTCAAGCTCTTGTTAGAATTATCGAAAGGATTGAAAGTGGGAGGTCTTAAATTCAAATCTAAACATGTTTCCATTTGATTTCTATGTTAAATTAGGTCACCATCATGTCTCAAAATGCACGAACAGACAGAACTTACTTTTGATAGATGACCTGGTGTAAATGCTCCTTTTAGGATGCTAAAACCTTAAACAAATCAATACCCACTTGACGAAAGAATTGTAGACAACATCTGAATTCTCAAAGTTATTCAGAACAGTGTTGCTTACTATTGTCAGGCTGTTGATACGGCAGTACGAGTTTTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCAGGAATTGCGGTAATGGGGCACGTTGGGCTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAACATCGCCAGTGCTGTTAAGGTCCCCCCCCCCACTCCCCTACTTCCCTCTTTTTATTAGTACTGTTTGCAGTTGTTTCTGGGATACAATTGCTGTTGTTATGTTGCATAGCTTGTCGTGTTCGGTTTGGCTGTGAAATGCTGAATGGTGTATCTTTTGCAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTCGCCGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCAGCTGCTGCTACAGCTGCTCTCCGCATTCCCACCATAGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTTAGTTGATTGGACAGACATCCTTTTACATGCCATATGCACATACACACGTACATACATATAGGACTGCCAACCGTATTTCCTTTCCTTGAGCTATTTGCTAGCTTCTCTTTGTTTGACCTTTGCATATAATTATTCTTTCAAACTATTTCTTTTTCCTCGAGTTCAGGACTCAGAGTTTCTTTCTGAGTTCTCAATTAGAGAAGCATCGAAGGTCATCCTTGTTTAGCTTCCCTATATGATCCTGTTCTATGGTTATTCGATTCTTGCACCATGAAATCCAAACAGACTGTTAAGTGTTTAAATTGTTGTGAAAATCCCTAGTGGTTCATTGTGCTAGACTGAACTGCAACTGTTTAACTTTAAATTGTTTCGAGAATCCCTAAGGAGTGTATGCATCTGAACATAGAGGAATATTCAAGATTAGAGAAATATATCTACTTGGTAACAAGTCATGGAGGCAAATAGCGATTTGTTAATATTTGGGTTCGGATGCCGCTCCCTCGAATTGAAAGGAGCTTAAGAAGAAGAAATAGACCTTGCATTTCACTCAACAAAGAGACGGGGTTTATGATTTATTCAATATTTCAGATAACTTGTTAGTTTTTCTCTTTAAAGAAATGTTGGTTTCATCATCCATACTTGTTTGATAAGCTTGTCCATTGTGCTGCTTGTTTTGTCTTCGATTACTTGACGACAATCATAGAGTCGAGTATCCTCTGCAGCTGGACTAACTAATTTCTTAAACACAGGTGTTAGTTTACCATGATCTACTCGGCATGCTTCAACACCCGCATCATGCTAAGGTTACCATCAGCCTGCACCTTTGCTTCGACTAAAGTTTCTATATGTTTGTTTAAGGTGTTTTAACTCCTGGATTGGATGTGCCATTGCAGGTGACCCCGAAATTCTGTAAGCAATTTGCACAAATTGGAGATGTCATCAACAAAGCTTTATCAGATTACAAGGAAGAGGTATCTAATGGTTCATTTCCCGGACCGCTCCATAGCCCATATAAGATCAGCGACGACGATTACAACGGTTTCTTGAAAGAGTTACGAAGTATGGGATTGGATGGGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCGATGATTAAACTCAGCTGCATTCACTGCCTCGCCCTCTTGGTCGTCTAGTCTTTCGAGTTTTAGATCATTTATGGAATTTATCAGTTGGGACAATTCGTTCTTAGAATCATTAGCTGAAGAACACTTGAACCACTAGAACACTATTATCCCCTTTAGTTATAGCTGATTTGAAGATGTGTTTGGATTCTGAACAAAGCCACTGCCACTGCCACAGCCACCGGATTCTGAACAATAAAAAAGATCTAATTGTATTTTTT

mRNA sequence

TTTAAAAGTTAGTTATATTTTAATTTTCATTCATTTTTTAAATTTTTATTTTAAAGTTTTAAGGATATGCATGAATTTGACACGTGTTGTCGGCGGGAGAATTCCACGTTTGCAAACCTGCAACTAATTCGCAGGCAACAGAGCAGCAACCAACCGATTGTGAAAATCCAATTCATTCGAAGACAAATTTCTCCCCATCAATGGCGCTCTTCAACCTCGTCACCAAGGCCGCAGTTCGTTCCACATCGCAATCGCTGCTCAACACGCTTCGTTGCATGAGCAATGTCCCTGAAAACACGGTCTACGGCGGCCCGACATCGAACACTCCAAACCAGAGAGTCACTCTCACCAATCTGAAACAAAAGTACAAGAAAGGCGAACCGATCACCATGGTCACTGCTTACGATTACCCCTCCGGTGTCCATTTGGATATGGCCGGCATTGATATCGCTTTGGTTGGCGATTCGGCTTCCATGGTTGTACACGGCCACGACACCACGTTGCCTATTACCTTGGATGAAATGCTTGTTCATTGCAGGTCTGTGGCTCGTGGGGCTAGGAGGCCGCTTTTGATCGGCGACTTGCCCTTTGGTACCTACGAATCCAGCTCCGTTCAGGCTGTTGATACGGCAGTACGAGTTTTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCAGGAATTGCGGTAATGGGGCACGTTGGGCTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAACATCGCCAGTGCTGTTAAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTCGCCGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCAGCTGCTGCTACAGCTGCTCTCCGCATTCCCACCATAGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGTTAGTTTACCATGATCTACTCGGCATGCTTCAACACCCGCATCATGCTAAGGTGACCCCGAAATTCTGTAAGCAATTTGCACAAATTGGAGATGTCATCAACAAAGCTTTATCAGATTACAAGGAAGAGGTATCTAATGGTTCATTTCCCGGACCGCTCCATAGCCCATATAAGATCAGCGACGACGATTACAACGGTTTCTTGAAAGAGTTACGAAGTATGGGATTGGATGGGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCGATGATTAAACTCAGCTGCATTCACTGCCTCGCCCTCTTGGTCGTCTAGTCTTTCGAGTTTTAGATCATTTATGGAATTTATCAGTTGGGACAATTCGTTCTTAGAATCATTAGCTGAAGAACACTTGAACCACTAGAACACTATTATCCCCTTTAGTTATAGCTGATTTGAAGATGTGTTTGGATTCTGAACAAAGCCACTGCCACTGCCACAGCCACCGGATTCTGAACAATAAAAAAGATCTAATTGTATTTTTT

Coding sequence (CDS)

ATGGCGCTCTTCAACCTCGTCACCAAGGCCGCAGTTCGTTCCACATCGCAATCGCTGCTCAACACGCTTCGTTGCATGAGCAATGTCCCTGAAAACACGGTCTACGGCGGCCCGACATCGAACACTCCAAACCAGAGAGTCACTCTCACCAATCTGAAACAAAAGTACAAGAAAGGCGAACCGATCACCATGGTCACTGCTTACGATTACCCCTCCGGTGTCCATTTGGATATGGCCGGCATTGATATCGCTTTGGTTGGCGATTCGGCTTCCATGGTTGTACACGGCCACGACACCACGTTGCCTATTACCTTGGATGAAATGCTTGTTCATTGCAGGTCTGTGGCTCGTGGGGCTAGGAGGCCGCTTTTGATCGGCGACTTGCCCTTTGGTACCTACGAATCCAGCTCCGTTCAGGCTGTTGATACGGCAGTACGAGTTTTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCAGGAATTGCGGTAATGGGGCACGTTGGGCTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAACATCGCCAGTGCTGTTAAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTCGCCGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCAGCTGCTGCTACAGCTGCTCTCCGCATTCCCACCATAGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGTTAGTTTACCATGATCTACTCGGCATGCTTCAACACCCGCATCATGCTAAGGTGACCCCGAAATTCTGTAAGCAATTTGCACAAATTGGAGATGTCATCAACAAAGCTTTATCAGATTACAAGGAAGAGGTATCTAATGGTTCATTTCCCGGACCGCTCCATAGCCCATATAAGATCAGCGACGACGATTACAACGGTTTCTTGAAAGAGTTACGAAGTATGGGATTGGATGGGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCGATGA

Protein sequence

MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGARRPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR
Homology
[Close]
BLAST of Cp4.1LG12g11020 vs. Swiss-Prot
Match: PANB2_ARATH (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana GN=KPHMT2 PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.2e-151
Identity = 268/334 (80.24%), Postives = 301/334 (90.12%), Query Frame = 1 

Query: 14  STSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSG 73
           S +   + T+R MSNVPE+TVYGGP     NQRVTLT L+QK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPI+L+EMLVHCR+VARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVRVLKEGGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPF 253
           GGFRPQGKNIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPTIGIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKAL +YKEEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
           PYKIS  D +GFL EL+ +GLD AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of Cp4.1LG12g11020 vs. Swiss-Prot
Match: PANB1_ARATH (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana GN=KPHMT1 PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 4.6e-151
Identity = 265/324 (81.79%), Postives = 298/324 (91.98%), Query Frame = 1 

Query: 22  TLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSGVHLDMAGI 81
           ++R MSN+PENTVYGGP    PNQRVTLT+L+QK+++GEPIT+VTAYDYPS VHLD AGI
Sbjct: 17  SVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGI 76

Query: 82  DIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGARRPLLIGDLPFGTYESSSVQAV 141
           D+ LVGDSASMVVHGHDTTLPI+LDEMLVHCR+VARGA+RPLL+GDLPFGTYESSS QAV
Sbjct: 77  DVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAV 136

Query: 142 DTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 201
           DTAVRVLKEGGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVLGGFRPQG+
Sbjct: 137 DTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGR 196

Query: 202 NIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPFCSGQVLVY 261
           NIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPTIGIGAGPFCSGQVLVY
Sbjct: 197 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVY 256

Query: 262 HDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHSPYKISDDD 321
           HDLLGM+QHPHHAKVTPKFCKQ+A +G+VINKAL +YKEEVS   FPGP HSPYKI+  +
Sbjct: 257 HDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASE 316

Query: 322 YNGFLKELRSMGLDGAASAAAAKA 346
            +GFL EL+ +G D AASAAA  A
Sbjct: 317 LDGFLTELQKLGFDKAASAAALAA 340

[Close]
BLAST of Cp4.1LG12g11020 vs. Swiss-Prot
Match: PANB1_ORYSJ (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativa subsp. japonica GN=KPHMT1 PE=2 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 6.4e-137
Identity = 248/334 (74.25%), Postives = 283/334 (84.73%), Query Frame = 1 

Query: 24  RCMSNVPENTVYGGPTSNT------------PNQRVTLTNLKQKYKKGEPITMVTAYDYP 83
           R +S+VPE+ VYGGP                  +RVT+T L+ K+++GEPIT+VTAYDYP
Sbjct: 18  RPLSHVPESAVYGGPRPQDVGAAAGAGAGAGATRRVTVTTLRGKHRRGEPITVVTAYDYP 77

Query: 84  SGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGARRPLLIGDLPFG 143
           S VH+D AGID+ LVGDSA+MVVHGHDTTLPI+LD ML HCR+VARGA RPLL+GDLPFG
Sbjct: 78  SAVHVDSAGIDVCLVGDSAAMVVHGHDTTLPISLDVMLEHCRAVARGATRPLLVGDLPFG 137

Query: 144 TYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAIS 203
            YESSS +AVD+AVRVLKEGGMDAIKLEGGA SRI+AA+AIVEAGIAVMGHVGLTPQAIS
Sbjct: 138 CYESSSTRAVDSAVRVLKEGGMDAIKLEGGAPSRISAAKAIVEAGIAVMGHVGLTPQAIS 197

Query: 204 VLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAG 263
           VLGGFRPQGK + SAVKVVETA++LQEAGCF+VVLECVP  VAAAAT+AL+IPTIGIGAG
Sbjct: 198 VLGGFRPQGKTVDSAVKVVETALALQEAGCFSVVLECVPAPVAAAATSALQIPTIGIGAG 257

Query: 264 PFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPL 323
           PFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQF  +G VINKALS+YK+EV   SFPGP 
Sbjct: 258 PFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQFGNVGHVINKALSEYKQEVETRSFPGPS 317

Query: 324 HSPYKISDDDYNGFLKELRSMGLDGAASAAAAKA 346
           H+PYKI+  D +GF   L+ MGLD AA+AAAA A
Sbjct: 318 HTPYKIAAADVDGFANALQKMGLDEAANAAAAAA 351

[Close]
BLAST of Cp4.1LG12g11020 vs. Swiss-Prot
Match: PANB2_ORYSJ (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica GN=KPHMT2 PE=3 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.2e-120
Identity = 222/326 (68.10%), Postives = 263/326 (80.67%), Query Frame = 1 

Query: 24  RCMSN-VPENTVYGG------PTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSGVHL 83
           R MSN   E  +YGG        S+   +RVTL  L+ K+++GEPI+MVTAYDYPSGVH+
Sbjct: 54  RAMSNGAAEPAIYGGGGGAQQAASSAAARRVTLATLRGKHRRGEPISMVTAYDYPSGVHV 113

Query: 84  DMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGARRPLLIGDLPFGTYESS 143
           D AG DI LVGDSA+MV HGHD TLPI+LD M+ HCR+VARGA R  L+GDLPFG+YE+S
Sbjct: 114 DAAGFDICLVGDSAAMVAHGHDNTLPISLDLMIEHCRAVARGAARTFLVGDLPFGSYEAS 173

Query: 144 SVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGF 203
           + QAV +AVRV+KEGG+++IKLEG A SRI+AARAIV+AGIAVMGH+GLTPQ++S LGGF
Sbjct: 174 TAQAVGSAVRVMKEGGVNSIKLEGSAPSRISAARAIVDAGIAVMGHIGLTPQSVSALGGF 233

Query: 204 RPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPFCSG 263
           RPQGK + SAVKVVE A++LQEAGCFAVVLECVP  VAAAAT+AL IPTIGIGAGPFCSG
Sbjct: 234 RPQGKTVESAVKVVEAALALQEAGCFAVVLECVPAPVAAAATSALTIPTIGIGAGPFCSG 293

Query: 264 QVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHSPYK 323
           QVLVYHDLLG  Q   HAKV+PKFCKQ+  IGDVIN+ALS YK+EV   SFPGP H+PYK
Sbjct: 294 QVLVYHDLLGTFQ-TSHAKVSPKFCKQYGNIGDVINRALSKYKQEVETQSFPGPSHTPYK 353

Query: 324 ISDDDYNGFLKELRSMGLDGAASAAA 343
           ++  D + FL  L+  GL+ AA AAA
Sbjct: 354 LAATDVDAFLNALKMKGLNVAADAAA 378

[Close]
BLAST of Cp4.1LG12g11020 vs. Swiss-Prot
Match: PANB_HELMI (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) GN=panB PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.5e-72
Identity = 138/269 (51.30%), Postives = 191/269 (71.00%), Query Frame = 1 

Query: 45  QRVTLTNLKQKYKKGEPITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPIT 104
           +++TL   K+  K+G+ + M+TAYDYP    +D + I+I LVGDS  MVV G+D+T+P+T
Sbjct: 3   KKITLPQCKEMKKQGKRLRMITAYDYPFARLVDESEIEIILVGDSLGMVVLGYDSTVPVT 62

Query: 105 LDEMLVHCRSVARGARRPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHS 164
           LDEM+ HC+ V RGA   L++ D+PFG+Y  S   A+  A R+LKE G++A+K+EGG   
Sbjct: 63  LDEMIHHCKPVVRGAPNTLVVADMPFGSYNVSKEDAIRNANRMLKESGIEAVKVEGGTRM 122

Query: 165 RITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAV 224
             T  RA+V+AGI VMGH+GLTPQ  + LGGF+ QGK   +A +++E A++L+ AG F++
Sbjct: 123 APTV-RALVDAGIPVMGHIGLTPQTAAQLGGFKVQGKTEDAAQQLLEDALALESAGAFSI 182

Query: 225 VLECVPPEVAAAATAALRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQF 284
           V+ECVP  +A   TA+L IPTIGIGAGP+C+GQVLV  DLLG+     + +  PKF KQ+
Sbjct: 183 VIECVPAGLARTITASLSIPTIGIGAGPYCNGQVLVIQDLLGI-----YDRFVPKFVKQY 242

Query: 285 AQIGDVINKALSDYKEEVSNGSFPGPLHS 314
           AQ G  I  AL+DY  EV++G FPGP HS
Sbjct: 243 AQTGPAIRAALNDYAREVADGVFPGPEHS 265

[Close]
BLAST of Cp4.1LG12g11020 vs. TrEMBL
Match: A0A0A0K8B2_CUCSA (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus GN=Csa_7G338120 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 5.8e-177
Identity = 318/347 (91.64%), Postives = 334/347 (96.25%), Query Frame = 1 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALF L+T A  R+ +QSLL TLR MSNVPENTVYGGPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPITLDE+LVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAVR+LKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD D+NGFLKEL++MGLD AASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of Cp4.1LG12g11020 vs. TrEMBL
Match: B9I8Q3_POPTR (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Populus trichocarpa GN=POPTR_0014s08620g PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 6.2e-155
Identity = 277/347 (79.83%), Postives = 312/347 (89.91%), Query Frame = 1 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           M L   +TK++      +LL  LR MSNVPENTVYGGP    P+QRVTLT+LKQK+KKG+
Sbjct: 1   MGLLTYLTKSSSTLKPTTLLRHLRFMSNVPENTVYGGPKPQNPHQRVTLTHLKQKHKKGD 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120
           PIT+VTAYDYPS VHLD AGID+ LVGDSASMVVHGHDTTLPI+LDEMLVHCR+VARGA+
Sbjct: 61  PITVVTAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAK 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QAVDTAVR+LKEGGMDAIKLEGG+ SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSNQAVDTAVRILKEGGMDAIKLEGGSPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETA++LQEAGCF+VVLECVP  VAAAAT+A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETALALQEAGCFSVVLECVPAPVAAAATSA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           L+IPTIGIGAGPFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+A +GDVINKAL +YKE
Sbjct: 241 LKIPTIGIGAGPFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAHVGDVINKALVEYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
           EV+NGSFPGP HSPYKIS+ + NGF+ EL+ +GLD AA++ AA A +
Sbjct: 301 EVANGSFPGPAHSPYKISETEINGFMSELQKLGLDKAAASTAAAAEK 347

[Close]
BLAST of Cp4.1LG12g11020 vs. TrEMBL
Match: A0A078K2Y5_BRANA (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Brassica napus GN=BnaAnng40200D PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.2e-153
Identity = 277/343 (80.76%), Postives = 309/343 (90.09%), Query Frame = 1 

Query: 7   VTKAAVRSTSQSL--LNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITM 66
           +  + VRS S+SL  +   R MSNVPENTVYGGP    PNQRVTLT L+QK+KKGE IT+
Sbjct: 1   MASSLVRSISRSLRTVTASRLMSNVPENTVYGGPKPQNPNQRVTLTQLRQKHKKGEAITV 60

Query: 67  VTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGARRPLL 126
           VTAYDYPS VH+D AGID+ LVGDSASMVVHGHDTTLPI+LDEMLVHCR+VARGA+RPLL
Sbjct: 61  VTAYDYPSAVHVDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLL 120

Query: 127 IGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVG 186
           +GDLPFG YESS++QAVD+AVRVLKEGGMDAIKLEGG+ SRITAAR+IVEAGIAVMGHVG
Sbjct: 121 VGDLPFGAYESSTIQAVDSAVRVLKEGGMDAIKLEGGSPSRITAARSIVEAGIAVMGHVG 180

Query: 187 LTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIP 246
           LTPQAISVLGGFRPQG+NIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IP
Sbjct: 181 LTPQAISVLGGFRPQGRNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALHIP 240

Query: 247 TIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSN 306
           TIGIGAGPFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKAL +YKEEVS 
Sbjct: 241 TIGIGAGPFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSK 300

Query: 307 GSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
            SFPGP HSPYKIS +D NGFL EL+ +GLD AASAAAA A +
Sbjct: 301 HSFPGPFHSPYKISSNDLNGFLSELQKLGLDEAASAAAASAEK 343

[Close]
BLAST of Cp4.1LG12g11020 vs. TrEMBL
Match: A0A067KNV3_JATCU (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Jatropha curcas GN=JCGZ_07886 PE=3 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 3.4e-153
Identity = 278/347 (80.12%), Postives = 311/347 (89.63%), Query Frame = 1 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALF  + +++    +  L + LR MSNVPENTVYGGP S  PN+RVTLT LKQK+KKGE
Sbjct: 1   MALFAKLYRSSSVIKAHYLHHCLRSMSNVPENTVYGGPKSQNPNERVTLTQLKQKHKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120
           PIT+VTAYDYPS VHLD AGIDI LVGDSA+MVVHGHDTTLPI+L+EMLVHCR+VARGA+
Sbjct: 61  PITVVTAYDYPSAVHLDQAGIDICLVGDSAAMVVHGHDTTLPISLEEMLVHCRAVARGAK 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESS+ QAVDTAVRVLKEGGMDAIKLEGG+ SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSTKQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI+SAVKVVET+++LQE GCF+VVLECVP  VAAAAT+A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNISSAVKVVETSLALQEVGCFSVVLECVPAPVAAAATSA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQ+AQ+GDVINKAL +YKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQYAQVGDVINKALLEYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
           EV+N SFPGP HSPYKI+  D NG L EL+ +GL+ AASAAAA A +
Sbjct: 301 EVTNSSFPGPAHSPYKINAADINGLLNELQKLGLNKAASAAAAAAEK 347

[Close]
BLAST of Cp4.1LG12g11020 vs. TrEMBL
Match: B9RDY4_RICCO (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Ricinus communis GN=RCOM_1616690 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 4.4e-153
Identity = 279/347 (80.40%), Postives = 308/347 (88.76%), Query Frame = 1 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           M+L   ++K++  +     L+ LR MSN+PENTVYGGP     NQRVTL++LKQK+KKGE
Sbjct: 1   MSLLTKLSKSSSITHPNYFLHCLRYMSNIPENTVYGGPKPQCSNQRVTLSHLKQKHKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120
           PIT+VTAYDY S VHLD AGIDI LVGDSA+MVVHGHDTTLPI+LDEMLVHCRSVARGA 
Sbjct: 61  PITVVTAYDYSSAVHLDTAGIDICLVGDSAAMVVHGHDTTLPISLDEMLVHCRSVARGAN 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESS++QAVDTAVRVLKEGGMDAIKLEGG+ SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSTMQAVDTAVRVLKEGGMDAIKLEGGSSSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI+SAVKVVETA+ LQEAGCF+VVLECVP  VAAAAT+A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNISSAVKVVETALQLQEAGCFSVVLECVPAPVAAAATSA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFA++GDVINKAL +YKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFARVGDVINKALLEYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
           EV NGSFPGP HSPYKIS  D NGFL EL+ +GLD A S AAA A +
Sbjct: 301 EVINGSFPGPAHSPYKISAADMNGFLSELQKLGLDKAVSLAAAAAEK 347

[Close]
BLAST of Cp4.1LG12g11020 vs. TAIR10
Match: AT3G61530.1 (AT3G61530.1 Phosphoenolpyruvate carboxylase family protein)

HSP 1 Score: 537.3 bits (1383), Expect = 6.8e-153
Identity = 268/334 (80.24%), Postives = 301/334 (90.12%), Query Frame = 1 

Query: 14  STSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSG 73
           S +   + T+R MSNVPE+TVYGGP     NQRVTLT L+QK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPI+L+EMLVHCR+VARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVRVLKEGGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPF 253
           GGFRPQGKNIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPTIGIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKAL +YKEEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
           PYKIS  D +GFL EL+ +GLD AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of Cp4.1LG12g11020 vs. TAIR10
Match: AT2G46110.1 (AT2G46110.1 ketopantoate hydroxymethyltransferase 1)

HSP 1 Score: 535.4 bits (1378), Expect = 2.6e-152
Identity = 265/324 (81.79%), Postives = 298/324 (91.98%), Query Frame = 1 

Query: 22  TLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSGVHLDMAGI 81
           ++R MSN+PENTVYGGP    PNQRVTLT+L+QK+++GEPIT+VTAYDYPS VHLD AGI
Sbjct: 17  SVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGI 76

Query: 82  DIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGARRPLLIGDLPFGTYESSSVQAV 141
           D+ LVGDSASMVVHGHDTTLPI+LDEMLVHCR+VARGA+RPLL+GDLPFGTYESSS QAV
Sbjct: 77  DVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAV 136

Query: 142 DTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 201
           DTAVRVLKEGGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVLGGFRPQG+
Sbjct: 137 DTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGR 196

Query: 202 NIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPFCSGQVLVY 261
           NIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPTIGIGAGPFCSGQVLVY
Sbjct: 197 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVY 256

Query: 262 HDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHSPYKISDDD 321
           HDLLGM+QHPHHAKVTPKFCKQ+A +G+VINKAL +YKEEVS   FPGP HSPYKI+  +
Sbjct: 257 HDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASE 316

Query: 322 YNGFLKELRSMGLDGAASAAAAKA 346
            +GFL EL+ +G D AASAAA  A
Sbjct: 317 LDGFLTELQKLGFDKAASAAALAA 340

[Close]
BLAST of Cp4.1LG12g11020 vs. NCBI nr
Match: gi|449443772|ref|XP_004139651.1| (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 628.2 bits (1619), Expect = 8.3e-177
Identity = 318/347 (91.64%), Postives = 334/347 (96.25%), Query Frame = 1 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALF L+T A  R+ +QSLL TLR MSNVPENTVYGGPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPITLDE+LVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAVR+LKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD D+NGFLKEL++MGLD AASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of Cp4.1LG12g11020 vs. NCBI nr
Match: gi|659092903|ref|XP_008447278.1| (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase-like [Cucumis melo])

HSP 1 Score: 620.2 bits (1598), Expect = 2.3e-174
Identity = 317/347 (91.35%), Postives = 330/347 (95.10%), Query Frame = 1 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALF L+T AA R+  QSLL TLR MSNVPENTVY GPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QAVDTAVR+LKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
           EVSN SFPGP HSPYKISD D NGFLKEL++MGLD AASAAAAKANR
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of Cp4.1LG12g11020 vs. NCBI nr
Match: gi|659098169|ref|XP_008450010.1| (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase-like [Cucumis melo])

HSP 1 Score: 592.8 bits (1527), Expect = 3.9e-166
Identity = 303/346 (87.57%), Postives = 327/346 (94.51%), Query Frame = 1 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MA+F L+T  A ++ +QSLL TLR MSNVPENTVYGGPTS TPNQRVTLTNL QKYKKGE
Sbjct: 1   MAVFKLLTWIASQTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLIQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGH++TLPI+LDEMLVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHNSTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RP LI DLPFGTYESS   AVDTAV++LKEG MDAIKLEGGAHSRITAARAIV+AGIAVM
Sbjct: 121 RPFLIVDLPFGTYESS--HAVDTAVKILKEGRMDAIKLEGGAHSRITAARAIVDAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAAT A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATVA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLG+LQHPHHAKVTPKFCKQFAQIGDVIN+ALS+Y+E
Sbjct: 241 LRIPTLGIGAGPFCSGQVLVYHDLLGILQHPHHAKVTPKFCKQFAQIGDVINQALSEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKAN 347
           EVSNGSFPGPLH+PYKISD D NGF+KEL++MGLD AASAA+AKA+
Sbjct: 301 EVSNGSFPGPLHNPYKISDADLNGFMKELQNMGLDEAASAASAKAS 344

[Close]
BLAST of Cp4.1LG12g11020 vs. NCBI nr
Match: gi|224130402|ref|XP_002320828.1| (ketopantoate hydroxymethyltransferase family protein [Populus trichocarpa])

HSP 1 Score: 555.1 bits (1429), Expect = 8.9e-155
Identity = 277/347 (79.83%), Postives = 312/347 (89.91%), Query Frame = 1 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           M L   +TK++      +LL  LR MSNVPENTVYGGP    P+QRVTLT+LKQK+KKG+
Sbjct: 1   MGLLTYLTKSSSTLKPTTLLRHLRFMSNVPENTVYGGPKPQNPHQRVTLTHLKQKHKKGD 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120
           PIT+VTAYDYPS VHLD AGID+ LVGDSASMVVHGHDTTLPI+LDEMLVHCR+VARGA+
Sbjct: 61  PITVVTAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAK 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QAVDTAVR+LKEGGMDAIKLEGG+ SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSNQAVDTAVRILKEGGMDAIKLEGGSPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETA++LQEAGCF+VVLECVP  VAAAAT+A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETALALQEAGCFSVVLECVPAPVAAAATSA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           L+IPTIGIGAGPFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+A +GDVINKAL +YKE
Sbjct: 241 LKIPTIGIGAGPFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAHVGDVINKALVEYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
           EV+NGSFPGP HSPYKIS+ + NGF+ EL+ +GLD AA++ AA A +
Sbjct: 301 EVANGSFPGPAHSPYKISETEINGFMSELQKLGLDKAAASTAAAAEK 347

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BLAST of Cp4.1LG12g11020 vs. NCBI nr
Match: gi|923716262|ref|XP_013663892.1| (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like isoform X2 [Brassica napus])

HSP 1 Score: 550.8 bits (1418), Expect = 1.7e-153
Identity = 277/343 (80.76%), Postives = 309/343 (90.09%), Query Frame = 1 

Query: 7   VTKAAVRSTSQSL--LNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITM 66
           +  + VRS S+SL  +   R MSNVPENTVYGGP    PNQRVTLT L+QK+KKGE IT+
Sbjct: 1   MASSLVRSISRSLRTVTASRLMSNVPENTVYGGPKPQNPNQRVTLTQLRQKHKKGEAITV 60

Query: 67  VTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGARRPLL 126
           VTAYDYPS VH+D AGID+ LVGDSASMVVHGHDTTLPI+LDEMLVHCR+VARGA+RPLL
Sbjct: 61  VTAYDYPSAVHVDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLL 120

Query: 127 IGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVG 186
           +GDLPFG YESS++QAVD+AVRVLKEGGMDAIKLEGG+ SRITAAR+IVEAGIAVMGHVG
Sbjct: 121 VGDLPFGAYESSTIQAVDSAVRVLKEGGMDAIKLEGGSPSRITAARSIVEAGIAVMGHVG 180

Query: 187 LTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIP 246
           LTPQAISVLGGFRPQG+NIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IP
Sbjct: 181 LTPQAISVLGGFRPQGRNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALHIP 240

Query: 247 TIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSN 306
           TIGIGAGPFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKAL +YKEEVS 
Sbjct: 241 TIGIGAGPFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSK 300

Query: 307 GSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 348
            SFPGP HSPYKIS +D NGFL EL+ +GLD AASAAAA A +
Sbjct: 301 HSFPGPFHSPYKISSNDLNGFLSELQKLGLDEAASAAAASAEK 343

The following BLAST results are available for this feature:
BLAST of Cp4.1LG12g11020 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PANB2_ARATH1.2e-15180.243-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis... [more]
PANB1_ARATH4.6e-15181.793-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis... [more]
PANB1_ORYSJ6.4e-13774.253-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativ... [more]
PANB2_ORYSJ4.2e-12068.103-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativ... [more]
PANB_HELMI2.5e-7251.303-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum... [more]
BLAST of Cp4.1LG12g11020 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K8B2_CUCSA5.8e-17791.643-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus GN=Csa_7G338... [more]
B9I8Q3_POPTR6.2e-15579.833-methyl-2-oxobutanoate hydroxymethyltransferase OS=Populus trichocarpa GN=POPTR... [more]
A0A078K2Y5_BRANA1.2e-15380.763-methyl-2-oxobutanoate hydroxymethyltransferase OS=Brassica napus GN=BnaAnng402... [more]
A0A067KNV3_JATCU3.4e-15380.123-methyl-2-oxobutanoate hydroxymethyltransferase OS=Jatropha curcas GN=JCGZ_0788... [more]
B9RDY4_RICCO4.4e-15380.403-methyl-2-oxobutanoate hydroxymethyltransferase OS=Ricinus communis GN=RCOM_161... [more]
BLAST of Cp4.1LG12g11020 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G61530.16.8e-15380.24 Phosphoenolpyruvate carboxylase family protein[more]
AT2G46110.12.6e-15281.79 ketopantoate hydroxymethyltransferase 1[more]
BLAST of Cp4.1LG12g11020 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449443772|ref|XP_004139651.1|8.3e-17791.64PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-lik... [more]
gi|659092903|ref|XP_008447278.1|2.3e-17491.35PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase-like [Cucumis melo][more]
gi|659098169|ref|XP_008450010.1|3.9e-16687.57PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase-like [Cucumis melo][more]
gi|224130402|ref|XP_002320828.1|8.9e-15579.83ketopantoate hydroxymethyltransferase family protein [Populus trichocarpa][more]
gi|923716262|ref|XP_013663892.1|1.7e-15380.76PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-lik... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:00038643-methyl-2-oxobutanoate hydroxymethyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0015940pantothenate biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR015813Pyrv/PenolPyrv_Kinase-like_dom
IPR003700Pantoate_hydroxy_MeTrfase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0015940 pantothenate biosynthetic process
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005575 cellular_component
molecular_function GO:0003864 3-methyl-2-oxobutanoate hydroxymethyltransferase activity
molecular_function GO:0050897 cobalt ion binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g11020.1Cp4.1LG12g11020.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003700Ketopantoate hydroxymethyltransferaseHAMAPMF_00156PanBcoord: 47..314
score: 39
IPR003700Ketopantoate hydroxymethyltransferasePANTHERPTHR208813-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 14..347
score: 9.1E
IPR003700Ketopantoate hydroxymethyltransferasePFAMPF02548Pantoate_transfcoord: 46..309
score: 6.3E
IPR003700Ketopantoate hydroxymethyltransferaseTIGRFAMsTIGR00222TIGR00222coord: 48..313
score: 2.1
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domainGENE3DG3DSA:3.20.20.60coord: 45..313
score: 3.3E
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domainunknownSSF51621Phosphoenolpyruvate/pyruvate domaincoord: 46..312
score: 8.64
NoneNo IPR availablePANTHERPTHR20881:SF03-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 14..347
score: 9.1E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG12g11020Cucsa.078360Cucumber (Gy14) v1cgycpeB0132
Cp4.1LG12g11020CmaCh17G012480Cucurbita maxima (Rimu)cmacpeB371
Cp4.1LG12g11020CmoCh17G012290Cucurbita moschata (Rifu)cmocpeB335
Cp4.1LG12g11020Cla014963Watermelon (97103) v1cpewmB135
Cp4.1LG12g11020Csa7G338120Cucumber (Chinese Long) v2cpecuB157
Cp4.1LG12g11020ClCG09G009090Watermelon (Charleston Gray)cpewcgB129
Cp4.1LG12g11020CSPI07G14130Wild cucumber (PI 183967)cpecpiB159
Cp4.1LG12g11020Lsi02G020760Bottle gourd (USVL1VR-Ls)cpelsiB111
Cp4.1LG12g11020CsaV3_7G025860Cucumber (Chinese Long) v3cpecucB0182
Cp4.1LG12g11020Bhi09G001150Wax gourdcpewgoB0204
Cp4.1LG12g11020CsGy7G012740Cucumber (Gy14) v2cgybcpeB900
Cp4.1LG12g11020Carg25536Silver-seed gourdcarcpeB0597
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG12g11020Cucurbita pepo (Zucchini)cpecpeB165
Cp4.1LG12g11020Cucurbita maxima (Rimu)cmacpeB899
Cp4.1LG12g11020Cucurbita moschata (Rifu)cmocpeB837
Cp4.1LG12g11020Melon (DHL92) v3.5.1cpemeB119
Cp4.1LG12g11020Melon (DHL92) v3.6.1cpemedB146
Cp4.1LG12g11020Silver-seed gourdcarcpeB0118
Cp4.1LG12g11020Wax gourdcpewgoB0209