Cp4.1LG12g10390 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG12g10390
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Gibberellin 2-beta-dioxygenase
Location	Cp4.1LG12 : 9437210 .. 9438589 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGATAGTGGAAGTTCCAGTGATAGACATTAGCTGTCAGAGCTTGACCGAATCCGCTGCATCTTCTCTACTTAGAGCGTGCAACGAATTTGGATTCTTCTTCATTAGAAATCATGGCCTTTTAAAGGAGTTCTACCAAAAACTCATATCCGGCGCCGACCAACTTTTTTCTTCACCAGAGGAGACCAAGCAAAAACTGACAATGGGAGTTTCCTCTTACACCCCGAGATTTGAGGTGTCGCCTTTCTTCGAAAGCTTCAAGGTTTCGGGACCCAACTTCTCTGCCTCGGCTGCTGTGCAGGGTTTCACTCAATCCCTATTCGGGCACCAAGCCACTGAATTCAGGTCATCTTTCTTTTTTCCTTTCTTCTTCTTCTAAGAATGTTAGCTATTGAAATTTGAATGTCTTCTCACATATTTAACGATTTTAAGAGAAATTATTCTAAGAAGTTTATACATCATGAGTGCTATACGGTTGGATCTATGATTTAATTTAAATTTTTGAGGTTTTAGATAAACTTTTACATTTTTGTACTTTTAAGGAGTTTTTTTGATAGAGATTAATCCCTAAAACCCTGAAATGCTTAATTTTGAATAATTTAAAGATAGGTTAAGTTATAAGATAACTTCTTGAAATTTTTAGAATTGAGTACTAGAGTTGAGTCGCTGAAGATTCAACGTGTTCACTAAAATTCTAAATTTTCGATTTTGTGTTCAATATATTAATGCGTGAAAGAATATAATAATCAATCATTTTTCGTCTTCTTCTTTATTTGGAGTTTGTATGTTCGAGTTTATTTGCAGCAATCTTCTAGATGAATATGGAAGCAAAATGAAAGAAGTATCGAAAAGAGTAGTGAAGTTATTACTAAACATCTTGGGGGATAATATTGAAAACAAATTCTATGATTCCGAGTTCAGCAACGGCTTCACTCCTCGAGACATGAATGAAGAAGAAGAAGAAGAAGTGTTCAAGCAGCACACTGACCTCAGCTATGTGACAATAGTGTTCCAAAATGACATTAGAGGGCTACATATGAGATTGAAAGGAGGAGATTGGATGGACGTAAGGCCGAGTGAGGACGATAACCTTGTGATGAACATTGGGGATTTTATGGAGGCTTGGAGCAATGGGAGGCTAAGATCTGCCGTACATAGAGTTGTGTTAAGGAAAAATAATATGAAGAGATTCTCTATGGCTTTCTTTTTGACACCCGAAGATAATGATAAAGAAATATATGCACCGAGTGAGGTTGAGGTTGTTGGAAAAGGGAAATCAAGACTTTATCGCCCATTTTCACCCCAACAATATAGGCTTTTTAAGGAACAGAATTATGAGAAAGGGAAATACCTGATCAAGGATATTATTGGAACATAA

mRNA sequence

ATGTCGATAGTGGAAGTTCCAGTGATAGACATTAGCTGTCAGAGCTTGACCGAATCCGCTGCATCTTCTCTACTTAGAGCGTGCAACGAATTTGGATTCTTCTTCATTAGAAATCATGGCCTTTTAAAGGAGTTCTACCAAAAACTCATATCCGGCGCCGACCAACTTTTTTCTTCACCAGAGGAGACCAAGCAAAAACTGACAATGGGAGTTTCCTCTTACACCCCGAGATTTGAGGTGTCGCCTTTCTTCGAAAGCTTCAAGGTTTCGGGACCCAACTTCTCTGCCTCGGCTGCTGTGCAGGGTTTCACTCAATCCCTATTCGGGCACCAAGCCACTGAATTCAGCAATCTTCTAGATGAATATGGAAGCAAAATGAAAGAAGTATCGAAAAGAGTAGTGAAGTTATTACTAAACATCTTGGGGGATAATATTGAAAACAAATTCTATGATTCCGAGTTCAGCAACGGCTTCACTCCTCGAGACATGAATGAAGAAGAAGAAGAAGAAGTGTTCAAGCAGCACACTGACCTCAGCTATGTGACAATAGTGTTCCAAAATGACATTAGAGGGCTACATATGAGATTGAAAGGAGGAGATTGGATGGACGTAAGGCCGAGTGAGGACGATAACCTTGTGATGAACATTGGGGATTTTATGGAGGCTTGGAGCAATGGGAGGCTAAGATCTGCCGTACATAGAGTTGTGTTAAGGAAAAATAATATGAAGAGATTCTCTATGGCTTTCTTTTTGACACCCGAAGATAATGATAAAGAAATATATGCACCGAGTGAGGTTGAGGTTGTTGGAAAAGGGAAATCAAGACTTTATCGCCCATTTTCACCCCAACAATATAGGCTTTTTAAGGAACAGAATTATGAGAAAGGGAAATACCTGATCAAGGATATTATTGGAACATAA

Coding sequence (CDS)

ATGTCGATAGTGGAAGTTCCAGTGATAGACATTAGCTGTCAGAGCTTGACCGAATCCGCTGCATCTTCTCTACTTAGAGCGTGCAACGAATTTGGATTCTTCTTCATTAGAAATCATGGCCTTTTAAAGGAGTTCTACCAAAAACTCATATCCGGCGCCGACCAACTTTTTTCTTCACCAGAGGAGACCAAGCAAAAACTGACAATGGGAGTTTCCTCTTACACCCCGAGATTTGAGGTGTCGCCTTTCTTCGAAAGCTTCAAGGTTTCGGGACCCAACTTCTCTGCCTCGGCTGCTGTGCAGGGTTTCACTCAATCCCTATTCGGGCACCAAGCCACTGAATTCAGCAATCTTCTAGATGAATATGGAAGCAAAATGAAAGAAGTATCGAAAAGAGTAGTGAAGTTATTACTAAACATCTTGGGGGATAATATTGAAAACAAATTCTATGATTCCGAGTTCAGCAACGGCTTCACTCCTCGAGACATGAATGAAGAAGAAGAAGAAGAAGTGTTCAAGCAGCACACTGACCTCAGCTATGTGACAATAGTGTTCCAAAATGACATTAGAGGGCTACATATGAGATTGAAAGGAGGAGATTGGATGGACGTAAGGCCGAGTGAGGACGATAACCTTGTGATGAACATTGGGGATTTTATGGAGGCTTGGAGCAATGGGAGGCTAAGATCTGCCGTACATAGAGTTGTGTTAAGGAAAAATAATATGAAGAGATTCTCTATGGCTTTCTTTTTGACACCCGAAGATAATGATAAAGAAATATATGCACCGAGTGAGGTTGAGGTTGTTGGAAAAGGGAAATCAAGACTTTATCGCCCATTTTCACCCCAACAATATAGGCTTTTTAAGGAACAGAATTATGAGAAAGGGAAATACCTGATCAAGGATATTATTGGAACATAA

Protein sequence

MSIVEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEETKQKLTMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDEYGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSNGFTPRDMNEEEEEEVFKQHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAVHRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQNYEKGKYLIKDIIGT

Homology

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BLAST of Cp4.1LG12g10390 vs. Swiss-Prot
Match: GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.7e-22
Identity = 92/306 (30.07%), Postives = 145/306 (47.39%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           + VP ID+S Q  T  A   +  AC + GFF + NHG+ +          +  F  P   
Sbjct: 61  LNVPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAG 120

Query: 64  KQKLTMG-------VSSYTPRFEVS-PFFE--SFKVSGPNFSASAAVQGFTQSLFGHQAT 123
           KQK            SS+T RF    P+ E  SF+ S  N S S  VQ +     G +  
Sbjct: 121 KQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDN-SGSRTVQDYFSDTLGQEFE 180

Query: 124 EFSNLLDEYGSKMKEVSKRVVKLLLNILGDNIE--NKFYDSEFS----NGFTPRDMNEEE 183
           +F  +  +Y   M  +S ++++LL   LG N +    F++   S    N + P      +
Sbjct: 181 QFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPP--CQTPD 240

Query: 184 EEEVFKQHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGR 243
                  H D S +TI+ Q+ + GL + +    W  +RP+     V+NIGD   A SNG 
Sbjct: 241 LTLGTGPHCDPSSLTILHQDHVNGLQVFVDN-QWQSIRPNPKA-FVVNIGDTFMALSNGI 300

Query: 244 LRSAVHRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRL 294
            +S +HR V+ + + ++ SMAFFL P+  DK +  PS  +++ K K+R Y  F+   +  
Sbjct: 301 FKSCLHRAVVNRESARK-SMAFFLCPK-KDKVVKPPS--DILEKMKTRKYPDFTWSMFLE 357

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BLAST of Cp4.1LG12g10390 vs. Swiss-Prot
Match: G2OX3_ARATH (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.5e-20
Identity = 87/304 (28.62%), Postives = 140/304 (46.05%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           V +PVID++       A + +++AC EFGFF + NHG+  +   +L   A   F+     
Sbjct: 25  VLIPVIDLT----DSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSL 84

Query: 64  KQKLTMGVSSYTPRFEVSPFFESFKVSGPN----------FSASAAVQGF-TQSLFGHQA 123
           K K               PF    K  GPN           +A+  ++   T ++F H  
Sbjct: 85  KDKAG----------PPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTP 144

Query: 124 TEFSNLLDEYGSKMKEVSKRVVKLL---LNI-----LGDNIENKFYDSEF-SNGFTPRDM 183
             F   ++EY  +MK +S + ++++   L I     L   ++ K  DS    N +  ++ 
Sbjct: 145 AIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEE 204

Query: 184 NEEEEEEVFKQHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAW 243
              +EE  F +HTD   ++++  ND  GL + +K G W+DV P      V+ +GD ++  
Sbjct: 205 TPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVL-VGDTLQVM 264

Query: 244 SNGRLRSAVHRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQ 288
           +NGR +S  HRVV       R SM +F  P  ++K   AP    +V K    LY  F+  
Sbjct: 265 TNGRFKSVKHRVV-TNTKRSRISMIYFAGPPLSEK--IAPLSC-LVPKQDDCLYNEFTWS 309

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BLAST of Cp4.1LG12g10390 vs. Swiss-Prot
Match: AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.6e-20
Identity = 79/293 (26.96%), Postives = 140/293 (47.78%), Query Frame = 1


Query: 5   EVPVIDISCQSLT------ESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFS 64
           ++PVID S Q+L       +   + +L+A  ++G F      L  E  + +    + LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 65  SPEETKQKLTMGVSSYTPR-FEVSPFFESFKVSGPNFSASAAVQGFTQSLF-GHQATEFS 124
            P  TKQ+    VSS     +     +ES  ++  N      V  FTQ L+  H     S
Sbjct: 73  LPIPTKQR---NVSSKPFHGYLCHNLYESLGINDANVLEK--VNDFTQQLWPDHGNKSIS 132

Query: 125 NLLDEYGSKMKEVSKRVVKLLL------NILGDNIENKFYDSEFSNGFTPRDMNEEEEEE 184
             +  +  ++ E+   V ++++      N + +++ + +Y +      +P D +++++EE
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 185 V---FKQHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGR 244
                + HTD + +TI+ Q  + GL ++ K   W+ V+PS+D  LVM +GD + A  NGR
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVM-VGDSLCALLNGR 252

Query: 245 LRSAVHRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPF 281
           L S  HRV++      R+S   F  P+     +   S  E+V K   R+++PF
Sbjct: 253 LHSPYHRVIM-TGKKTRYSTGLFSIPKTG---VIIDSPEELVDKEHPRIFKPF 295

[Close]

BLAST of Cp4.1LG12g10390 vs. Swiss-Prot
Match: G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.6e-20
Identity = 85/307 (27.69%), Postives = 144/307 (46.91%), Query Frame = 1


Query: 2   SIVEVPVIDISCQSLTESAASSLLR-ACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSP 61
           S   VPVID     L +  AS L+  AC  +G + + NHG+     + +      LFS P
Sbjct: 52  STTTVPVID-----LNDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLP 111

Query: 62  EETKQKLTM---GVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSN 121
              K K T    GVS Y     +S FF    +    F+   +     + L+    T F +
Sbjct: 112 SHQKHKATRSPDGVSGYGIA-RISSFFPKL-MWYEGFTIVGSPLDHFRELWPQDYTRFCD 171

Query: 122 LLDEYGSKMKEVSKRVVKLLLNILG---DNIENKFYDSEFSNGFTPRDMN------EEEE 181
           ++ +Y   MK+++  ++ L+L+ LG   ++I+     ++F        +N      + + 
Sbjct: 172 IVVQYDETMKKLAGTLMCLMLDSLGITKEDIKWAGSKAQFEKACAALQLNSYPSCPDPDH 231

Query: 182 EEVFKQHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRL 241
                 HTD +++TI+ QNDI GL +  +G  W+ V P +   LV+N+GD     SNG  
Sbjct: 232 AMGLAPHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQ-GGLVVNVGDLFHILSNGLY 291

Query: 242 RSAVHRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLF 296
            S +HRV++ +   +RFS+A+   P  N   +      +++G  K  LYR  +  +Y   
Sbjct: 292 PSVLHRVLVNRTR-QRFSVAYLYGPPSN---VEICPHAKLIGPTKPPLYRSVTWNEYLGT 346

[Close]

BLAST of Cp4.1LG12g10390 vs. Swiss-Prot
Match: AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 9.6e-20
Identity = 77/290 (26.55%), Postives = 137/290 (47.24%), Query Frame = 1


Query: 5   EVPVIDISCQSLT------ESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFS 64
           ++PVID S Q+L       +   + +L+A  ++G F      L  E  + +    + LF 
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 65  SPEETKQKLTMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLF-GHQATEFSN 124
            P  TKQ+     S     +     +ES  ++  N      V  FTQ L+  H     S 
Sbjct: 66  LPIPTKQRNVS--SKLFHGYLCHNLYESLGINDANVLEK--VNDFTQQLWPDHGNKSISE 125

Query: 125 LLDEYGSKMKEVSKRVVKLLLNILG------DNIENKFYDSEFSNGFTPRDMNEEEEEEV 184
            +  +  ++ E+   V ++++   G      +++ + +Y +      +P D +++EE ++
Sbjct: 126 TIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETKL 185

Query: 185 -FKQHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRS 244
             + HTD + +TI+ Q  + GL ++ K   W+ V+PS+D  LVM +GD + A  NGRL S
Sbjct: 186 GLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVM-VGDSLCALLNGRLHS 245

Query: 245 AVHRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPF 281
             HRV++      R+S   F  P+     +   S  E+V K   R+++PF
Sbjct: 246 PYHRVIM-TGKKTRYSTGLFSIPKTG---VIIDSPEELVDKEHPRIFKPF 286

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BLAST of Cp4.1LG12g10390 vs. TrEMBL
Match: A0A0A0KW97_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.9e-83
Identity = 169/303 (55.78%), Postives = 230/303 (75.91%), Query Frame = 1


Query: 5   EVPVIDI-SCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSS-PEE 64
           E+ V+D+ SC+ L+ES+  SL +ACNE+G F+I+NHG+ KE YQKL +  D+L ++ PEE
Sbjct: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70

Query: 65  TKQ-KLTMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 124
           TK+ KL +G S YTPRF +SP+ ESFK  GPNFS  A+  GFT+ +FG + T+F  LLDE
Sbjct: 71  TKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDE 130

Query: 125 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSN--------GFTPRDMNEEEEEEVFK 184
           YGS M E+S+R++KLLL  +GDN+E+KFY+SEFSN         + PR+ NEE E   F 
Sbjct: 131 YGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIE--AFG 190

Query: 185 QHTDLSYVTIVFQNDIRGLHMRLKGGD-WMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAV 244
           +HTD+S VTI+FQ++I G+ M+ K GD W+DVRP E D L++NIGDF++AWSN RLRSA 
Sbjct: 191 KHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLE-DALLVNIGDFLQAWSNERLRSAE 250

Query: 245 HRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQN 296
           HRVVL K ++KRFS+AFFL  +D+D+E+YAPS  EVVG+G +R+Y+PFS ++YR ++E N
Sbjct: 251 HRVVL-KQDVKRFSLAFFLIFKDDDRELYAPS--EVVGEGNTRIYKPFSTKEYRAYRENN 307

[Close]

BLAST of Cp4.1LG12g10390 vs. TrEMBL
Match: B9R9V9_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1500930 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.1e-66
Identity = 153/305 (50.16%), Postives = 206/305 (67.54%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           +++P +DIS Q L++S+ SSL  AC E+GFF I NHG+ K+ Y+KL   AD LF+ P E+
Sbjct: 8   LQLPALDIS-QPLSQSSLSSLSSACKEWGFFRIINHGVSKDLYRKLYFLADHLFNLPCES 67

Query: 64  KQKL--TMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 123
           K K+  +  + +YTP F  SPFFES +VSGP+F ASA  Q  +Q LF H   EFS  + E
Sbjct: 68  KIKIGPSSSIKTYTPHFIASPFFESLRVSGPDFFASA--QTSSQVLFHHPNPEFSEAMSE 127

Query: 124 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFS--------NGFTPRDMNEEEEEEVFK 183
           YG+KM E+SKR+VK +L  +GD+   KFY+S+F+        N ++P +    EE E   
Sbjct: 128 YGNKMSELSKRIVKAVLISMGDDFAEKFYESDFNKCHGYMRINKYSPAE--SAEEVEGLG 187

Query: 184 QHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAVH 243
            HTD+S +TIV Q+DI GL +R K G WMD+ P E D LV+NIGD M AWSNG+LRS+ H
Sbjct: 188 MHTDMSCMTIVCQDDIGGLQVRSKLGKWMDINPCE-DTLVVNIGDLMHAWSNGKLRSSEH 247

Query: 244 RVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQNY 299
           RVVLR+  + RFS+AFF   ED DK I+AP+  EVVG+G  R+Y+PF  + Y  F+E + 
Sbjct: 248 RVVLRR-QVHRFSLAFFWCFED-DKVIFAPN--EVVGEGNLRMYKPFVCRDYLNFRESS- 301

[Close]

BLAST of Cp4.1LG12g10390 vs. TrEMBL
Match: A0A061FZ75_THECC (2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao GN=TCM_014890 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 3.1e-65
Identity = 154/318 (48.43%), Postives = 206/318 (64.78%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           VE+PV D+S Q L+ S+ SSL  AC E+GFF I NHG+ K+ Y+KL S ++ LFS P+E+
Sbjct: 8   VELPVFDLS-QPLSPSSLSSLSLACREWGFFRITNHGVSKDLYRKLYSLSNHLFSLPDES 67

Query: 64  KQKL--TMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 123
           K KL  T  + +YTP F  SPFFES +VSGP+F ASA  Q     LF    +EFS +L E
Sbjct: 68  KLKLGPTSSLKTYTPHFIASPFFESLRVSGPDFFASA--QSSVDVLFEQPNSEFSEVLKE 127

Query: 124 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSN--------GFTPRDMNEEEEEEVFK 183
           YGS M ++SK +V+ +L  LG+ +E K ++SEF N         +TP    +EEE +   
Sbjct: 128 YGSLMTKLSKEIVEAILKSLGEELEKKSFESEFGNCHGYLRINNYTPPKSMKEEEVDGLG 187

Query: 184 QHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAVH 243
            HTD+S VTIV+Q++I GL +R K G WMD+ P E D LV+NIGD M AWSNG+L S+ H
Sbjct: 188 MHTDMSCVTIVYQDEIGGLQVRSKEGKWMDINPCE-DTLVVNIGDLMHAWSNGKLSSSEH 247

Query: 244 RVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQNY 303
           RVVL K  + RFS+AFF   ED +K I+AP+  EVVG+   R+Y+PF    Y  F+ Q+ 
Sbjct: 248 RVVL-KRIVNRFSLAFFWCFED-EKVIFAPN--EVVGEDNLRIYKPFVCADYLKFR-QSS 307

Query: 304 EKGK-----YLIKDIIGT 307
           EKG+     + +KD  GT
Sbjct: 308 EKGRFDKVGFTVKDFAGT 316

[Close]

BLAST of Cp4.1LG12g10390 vs. TrEMBL
Match: D7TVY9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03440 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 4.0e-65
Identity = 145/313 (46.33%), Postives = 205/313 (65.50%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           +++PV+D+S Q +  S  +SL +AC E+GFF + NHG+ ++ + K+   +  +FS P ++
Sbjct: 8   IQLPVLDLS-QPVHPSFLTSLSQACEEWGFFHVMNHGIPQDLFSKVCLLSKHIFSLPADS 67

Query: 64  KQKL--TMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 123
           K KL  +  + +YTPRF  SPFFES +++GP+F ASA  +     LF  Q +EFS  L E
Sbjct: 68  KLKLGPSSSIKTYTPRFIASPFFESLRIAGPDFLASA--KSSVAELFDQQNSEFSETLQE 127

Query: 124 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSN--------GFTPRDMNEEEEEEVFK 183
           YGSKM E+SKR+V+ +L  LGD  E KF +SEF+N         ++P +  E+EE E   
Sbjct: 128 YGSKMVELSKRIVEAVLMSLGDGYERKFNESEFNNCQGYLRLVNYSPPECVEDEEVEGLG 187

Query: 184 QHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAVH 243
            HTD+S +TIV+Q++I GL MR KGG+W+D+ P E   LV+NIGD M+ WSNGRLRS+ H
Sbjct: 188 MHTDMSCITIVYQDEIGGLQMRSKGGEWIDITPCEG-TLVVNIGDLMQGWSNGRLRSSEH 247

Query: 244 RVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQN- 303
           RVVL K  + R S+AFF   ED +K + AP   E+VG+G SR+Y+PF   +Y  F+E N 
Sbjct: 248 RVVL-KRFVNRLSLAFFWCFED-EKVVLAPD--EIVGEGNSRIYKPFVCLEYLKFRESNE 307

[Close]

BLAST of Cp4.1LG12g10390 vs. TrEMBL
Match: B9RDS1_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1615250 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.2e-64
Identity = 147/316 (46.52%), Postives = 204/316 (64.56%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           VE+P++DIS + + +S+ SSL  AC E+GFF I NHG+ K+ Y K+   +  +FS P E 
Sbjct: 8   VELPILDIS-KPINQSSLSSLAEACKEWGFFNITNHGISKQLYNKIYLLSQNIFSLPSEI 67

Query: 64  KQKL--TMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 123
           K KL  +  + +YTP F  SP+FES +VSGPNF ASA  Q     LF  Q +EFS++L E
Sbjct: 68  KFKLGPSSSIKTYTPHFIASPYFESLRVSGPNFLASA--QSSADVLFNQQNSEFSDVLQE 127

Query: 124 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSN--------GFTPRDMNEEEEEEVFK 183
           YGSKM E+SKR+V++LL  LGD ++ KFY++EF+N         +TP   N E+E E   
Sbjct: 128 YGSKMTELSKRIVEMLLLSLGDGLDKKFYETEFNNCHGYLRIVNYTP-PKNLEDEVEGLG 187

Query: 184 QHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAVH 243
            HTD+S +TIV+Q++I GL ++ + G WMD+ P E + LV+NIGD ++AWSN + RS+ H
Sbjct: 188 MHTDMSCITIVYQDEIGGLQVKSREGKWMDISPCE-ETLVVNIGDMLQAWSNEKFRSSEH 247

Query: 244 RVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQN- 303
           +VVL K +  R S+AFF   ED  K I AP   EVVG+G  R+Y+PF    Y  F+E N 
Sbjct: 248 QVVL-KQSANRLSLAFFWCFED-QKVISAPD--EVVGEGCGRIYKPFVCSDYLKFRESNE 307

Query: 304 ---YEKGKYLIKDIIG 306
              +EK  + +KD  G
Sbjct: 308 RGKFEKVGFTVKDFAG 314

[Close]

BLAST of Cp4.1LG12g10390 vs. TAIR10
Match: AT4G23340.1 (AT4G23340.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 238.4 bits (607), Expect = 5.7e-63
Identity = 145/320 (45.31%), Postives = 201/320 (62.81%), Query Frame = 1


Query: 2   SIVEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPE 61
           S +++PV+D++ Q +  S  SSL  AC E+GFF++ NHG+ KE + K+ S +  +F +P 
Sbjct: 6   SSLQLPVLDLT-QPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKAPL 65

Query: 62  ETKQKLTMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLF-GHQATEFSNLLD 121
           E+K KL  G  SYTPR+  SP+FES  VSGP+FS SA        LF  H   E    + 
Sbjct: 66  ESKLKL--GPISYTPRYIASPYFESLVVSGPDFSDSAKASA--DVLFQDHHKPELRETMQ 125

Query: 122 EYGSKMKEVSKRVVKLLLNI-LGDNIENKFYDSEFSN--------GFTPRDMNEEEEE-- 181
           EYG+KM E+SKR++K+LL + LGD    + Y ++FSN         +TP    E++EE  
Sbjct: 126 EYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELV 185

Query: 182 EVFKQHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLR 241
           E    HTD+S +TIV+Q+ + GL MR K G W+D+ P  +D LV+NIGD M+AWSNGRLR
Sbjct: 186 EGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC-NDFLVVNIGDLMQAWSNGRLR 245

Query: 242 SAVHRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFK 301
           S+ HRVVLRK  + R S+AFFL  ED +K I AP   E+VG+GK R Y+ F   +Y  F+
Sbjct: 246 SSEHRVVLRK-LVNRVSLAFFLCFED-EKVILAPQ--EIVGEGKQRSYKSFKCSEYLKFR 305

Query: 302 EQN----YEKGKYLIKDIIG 306
           + N    +EK  + +KD  G
Sbjct: 306 QSNEEGKFEKIGFTVKDFAG 315

[Close]

BLAST of Cp4.1LG12g10390 vs. TAIR10
Match: AT5G51310.1 (AT5G51310.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 220.3 bits (560), Expect = 1.6e-57
Identity = 136/316 (43.04%), Postives = 202/316 (63.92%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           +E+PV DIS + L+ES+ +SL  AC E+GFF++ NHG+ ++ Y+KL   +  +F   +E 
Sbjct: 3   LELPVFDIS-KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 62

Query: 64  KQKLTMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQAT--EFSNLLDE 123
           K K  MG S+YTPRF  SPFFES +VSGP+F ASA     +   F  QAT  EFS L+ E
Sbjct: 63  KMK--MGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLMKE 122

Query: 124 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSN--GF-------TPRDMNEE----EE 183
           YG KM ++ ++++K +L+  GD++ +K+Y+SEF N  G+        P D  ++    +E
Sbjct: 123 YGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDE 182

Query: 184 EEVFK---QHTDLSYVTIVFQNDIRGLHMRLKGG-DWMDVRPSEDDNLVMNIGDFMEAWS 243
           +++ +    HTD+S +TIV Q+DI GL +R + G   MD+ P +D+ LV+N+GD + AW+
Sbjct: 183 QDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINP-KDEALVVNVGDLLHAWT 242

Query: 244 NGRLRSAVHRVVLRKNNM--KRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSP 299
           NGRLRS+ HRV+L++      RFS+AFF   +D  K ++AP EV V G    R++R F  
Sbjct: 243 NGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDG-KVVFAPDEV-VGGCEGMRVFRSFKC 302

[Close]

BLAST of Cp4.1LG12g10390 vs. TAIR10
Match: AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)

HSP 1 Score: 107.5 bits (267), Expect = 1.5e-23
Identity = 92/306 (30.07%), Postives = 145/306 (47.39%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           + VP ID+S Q  T  A   +  AC + GFF + NHG+ +          +  F  P   
Sbjct: 61  LNVPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAG 120

Query: 64  KQKLTMG-------VSSYTPRFEVS-PFFE--SFKVSGPNFSASAAVQGFTQSLFGHQAT 123
           KQK            SS+T RF    P+ E  SF+ S  N S S  VQ +     G +  
Sbjct: 121 KQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDN-SGSRTVQDYFSDTLGQEFE 180

Query: 124 EFSNLLDEYGSKMKEVSKRVVKLLLNILGDNIE--NKFYDSEFS----NGFTPRDMNEEE 183
           +F  +  +Y   M  +S ++++LL   LG N +    F++   S    N + P      +
Sbjct: 181 QFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPP--CQTPD 240

Query: 184 EEEVFKQHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGR 243
                  H D S +TI+ Q+ + GL + +    W  +RP+     V+NIGD   A SNG 
Sbjct: 241 LTLGTGPHCDPSSLTILHQDHVNGLQVFVDN-QWQSIRPNPKA-FVVNIGDTFMALSNGI 300

Query: 244 LRSAVHRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRL 294
            +S +HR V+ + + ++ SMAFFL P+  DK +  PS  +++ K K+R Y  F+   +  
Sbjct: 301 FKSCLHRAVVNRESARK-SMAFFLCPK-KDKVVKPPS--DILEKMKTRKYPDFTWSMFLE 357

[Close]

BLAST of Cp4.1LG12g10390 vs. TAIR10
Match: AT1G78550.1 (AT1G78550.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 102.8 bits (255), Expect = 3.8e-22
Identity = 88/293 (30.03%), Postives = 148/293 (50.51%), Query Frame = 1


Query: 5   EVPVIDIS--CQ-SLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPE 64
           E+PVID++  C  S  +S    L  AC ++GFF + NHG+   F +KL +   + F+ P 
Sbjct: 52  EIPVIDMTRLCSVSAMDSELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLPM 111

Query: 65  ETKQKLTMGVSSYTPRFEVSPFFESFKVS-GPNF-SASAAVQGFTQSLFGHQATEFSNLL 124
           + KQKL      +    +V+   E+ K+  G  F   +  ++     LF      F   L
Sbjct: 112 KEKQKLWQRSGEFEGFGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETL 171

Query: 125 DEYGSKMKEVSKRV---VKLLLNILGDNIENKFYD---SEFSNGFTPRDMNEEEEEEVFK 184
           + Y S++K ++K +   +  +L I  + +E+ F D   S   N + P    + ++     
Sbjct: 172 ETYSSEVKSIAKILFAKMASVLEIKHEEMEDLFDDVWQSIKINYYPP--CPQPDQVMGLT 231

Query: 185 QHTDLSYVTIVFQ-NDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAV 244
           QH+D + +TI+ Q N + GL ++ K G W+ V+P   D LV+N+G+ +E  +NGR RS  
Sbjct: 232 QHSDAAGLTILLQVNQVEGLQIK-KDGKWVVVKPLR-DALVVNVGEILEIITNGRYRSIE 291

Query: 245 HRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQY 286
           HR V+  +  +R S+A F +P    KE        +V + K  L++  S Q+Y
Sbjct: 292 HRAVV-NSEKERLSVAMFHSP---GKETIIRPAKSLVDRQKQCLFKSMSTQEY 336

[Close]

BLAST of Cp4.1LG12g10390 vs. TAIR10
Match: AT2G34555.1 (AT2G34555.1 gibberellin 2-oxidase 3)

HSP 1 Score: 100.9 bits (250), Expect = 1.4e-21
Identity = 87/304 (28.62%), Postives = 140/304 (46.05%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           V +PVID++       A + +++AC EFGFF + NHG+  +   +L   A   F+     
Sbjct: 25  VLIPVIDLT----DSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSL 84

Query: 64  KQKLTMGVSSYTPRFEVSPFFESFKVSGPN----------FSASAAVQGF-TQSLFGHQA 123
           K K               PF    K  GPN           +A+  ++   T ++F H  
Sbjct: 85  KDKAG----------PPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTP 144

Query: 124 TEFSNLLDEYGSKMKEVSKRVVKLL---LNI-----LGDNIENKFYDSEF-SNGFTPRDM 183
             F   ++EY  +MK +S + ++++   L I     L   ++ K  DS    N +  ++ 
Sbjct: 145 AIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEE 204

Query: 184 NEEEEEEVFKQHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAW 243
              +EE  F +HTD   ++++  ND  GL + +K G W+DV P      V+ +GD ++  
Sbjct: 205 TPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVL-VGDTLQVM 264

Query: 244 SNGRLRSAVHRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQ 288
           +NGR +S  HRVV       R SM +F  P  ++K   AP    +V K    LY  F+  
Sbjct: 265 TNGRFKSVKHRVV-TNTKRSRISMIYFAGPPLSEK--IAPLSC-LVPKQDDCLYNEFTWS 309

[Close]

BLAST of Cp4.1LG12g10390 vs. NCBI nr
Match: gi|449458351|ref|XP_004146911.1| (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus])

HSP 1 Score: 317.4 bits (812), Expect = 2.8e-83
Identity = 169/303 (55.78%), Postives = 230/303 (75.91%), Query Frame = 1


Query: 5   EVPVIDI-SCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSS-PEE 64
           E+ V+D+ SC+ L+ES+  SL +ACNE+G F+I+NHG+ KE YQKL +  D+L ++ PEE
Sbjct: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70

Query: 65  TKQ-KLTMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 124
           TK+ KL +G S YTPRF +SP+ ESFK  GPNFS  A+  GFT+ +FG + T+F  LLDE
Sbjct: 71  TKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDE 130

Query: 125 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSN--------GFTPRDMNEEEEEEVFK 184
           YGS M E+S+R++KLLL  +GDN+E+KFY+SEFSN         + PR+ NEE E   F 
Sbjct: 131 YGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIE--AFG 190

Query: 185 QHTDLSYVTIVFQNDIRGLHMRLKGGD-WMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAV 244
           +HTD+S VTI+FQ++I G+ M+ K GD W+DVRP E D L++NIGDF++AWSN RLRSA 
Sbjct: 191 KHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLE-DALLVNIGDFLQAWSNERLRSAE 250

Query: 245 HRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQN 296
           HRVVL K ++KRFS+AFFL  +D+D+E+YAPS  EVVG+G +R+Y+PFS ++YR ++E N
Sbjct: 251 HRVVL-KQDVKRFSLAFFLIFKDDDRELYAPS--EVVGEGNTRIYKPFSTKEYRAYRENN 307

[Close]

BLAST of Cp4.1LG12g10390 vs. NCBI nr
Match: gi|659107869|ref|XP_008453897.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo])

HSP 1 Score: 314.3 bits (804), Expect = 2.3e-82
Identity = 169/303 (55.78%), Postives = 229/303 (75.58%), Query Frame = 1


Query: 5   EVPVIDISC-QSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSS-PEE 64
           E+PV+D++C + L+ES+  SL +ACNE+G F+I+NHG+ KE Y KL +  D+L ++ PEE
Sbjct: 11  ELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAVPEE 70

Query: 65  TKQ-KLTMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 124
            K+ KL +GVS YTPRF++SP+ ESFK  GP FS SA+  GFT+ +FGH+ T+F NLLDE
Sbjct: 71  AKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQFRNLLDE 130

Query: 125 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSN--------GFTPRDMNEEEEEEVFK 184
           YGS M E+S+R++KLLL ++GD+ E+KFY+SEFSN         + PR+ NEE E   F 
Sbjct: 131 YGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEEIE--AFG 190

Query: 185 QHTDLSYVTIVFQNDIRGLHMRLKGGD-WMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAV 244
           +HTD+S VTI+FQ++I G+ M+ K GD W+DVRP E D L++NIGDF++AWSN RLRSA 
Sbjct: 191 KHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLE-DALLVNIGDFLQAWSNERLRSAE 250

Query: 245 HRVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQN 296
           HRVVL K N KRF++ FFL   D+D+E+YAPS  EVVG+G  R+Y+PFS ++YR F+E N
Sbjct: 251 HRVVL-KQNAKRFTLGFFLVFGDDDRELYAPS--EVVGEGNRRVYKPFSTKEYRAFRENN 307

[Close]

BLAST of Cp4.1LG12g10390 vs. NCBI nr
Match: gi|255539839|ref|XP_002510984.1| (PREDICTED: gibberellin 2-beta-dioxygenase 7 [Ricinus communis])

HSP 1 Score: 258.8 bits (660), Expect = 1.2e-65
Identity = 153/305 (50.16%), Postives = 206/305 (67.54%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           +++P +DIS Q L++S+ SSL  AC E+GFF I NHG+ K+ Y+KL   AD LF+ P E+
Sbjct: 8   LQLPALDIS-QPLSQSSLSSLSSACKEWGFFRIINHGVSKDLYRKLYFLADHLFNLPCES 67

Query: 64  KQKL--TMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 123
           K K+  +  + +YTP F  SPFFES +VSGP+F ASA  Q  +Q LF H   EFS  + E
Sbjct: 68  KIKIGPSSSIKTYTPHFIASPFFESLRVSGPDFFASA--QTSSQVLFHHPNPEFSEAMSE 127

Query: 124 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFS--------NGFTPRDMNEEEEEEVFK 183
           YG+KM E+SKR+VK +L  +GD+   KFY+S+F+        N ++P +    EE E   
Sbjct: 128 YGNKMSELSKRIVKAVLISMGDDFAEKFYESDFNKCHGYMRINKYSPAE--SAEEVEGLG 187

Query: 184 QHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAVH 243
            HTD+S +TIV Q+DI GL +R K G WMD+ P E D LV+NIGD M AWSNG+LRS+ H
Sbjct: 188 MHTDMSCMTIVCQDDIGGLQVRSKLGKWMDINPCE-DTLVVNIGDLMHAWSNGKLRSSEH 247

Query: 244 RVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQNY 299
           RVVLR+  + RFS+AFF   ED DK I+AP+  EVVG+G  R+Y+PF  + Y  F+E + 
Sbjct: 248 RVVLRR-QVHRFSLAFFWCFED-DKVIFAPN--EVVGEGNLRMYKPFVCRDYLNFRESS- 301

[Close]

BLAST of Cp4.1LG12g10390 vs. NCBI nr
Match: gi|590671448|ref|XP_007038334.1| (2-oxoglutarate-dependent dioxygenase [Theobroma cacao])

HSP 1 Score: 256.9 bits (655), Expect = 4.4e-65
Identity = 154/318 (48.43%), Postives = 206/318 (64.78%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           VE+PV D+S Q L+ S+ SSL  AC E+GFF I NHG+ K+ Y+KL S ++ LFS P+E+
Sbjct: 8   VELPVFDLS-QPLSPSSLSSLSLACREWGFFRITNHGVSKDLYRKLYSLSNHLFSLPDES 67

Query: 64  KQKL--TMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 123
           K KL  T  + +YTP F  SPFFES +VSGP+F ASA  Q     LF    +EFS +L E
Sbjct: 68  KLKLGPTSSLKTYTPHFIASPFFESLRVSGPDFFASA--QSSVDVLFEQPNSEFSEVLKE 127

Query: 124 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSN--------GFTPRDMNEEEEEEVFK 183
           YGS M ++SK +V+ +L  LG+ +E K ++SEF N         +TP    +EEE +   
Sbjct: 128 YGSLMTKLSKEIVEAILKSLGEELEKKSFESEFGNCHGYLRINNYTPPKSMKEEEVDGLG 187

Query: 184 QHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAVH 243
            HTD+S VTIV+Q++I GL +R K G WMD+ P E D LV+NIGD M AWSNG+L S+ H
Sbjct: 188 MHTDMSCVTIVYQDEIGGLQVRSKEGKWMDINPCE-DTLVVNIGDLMHAWSNGKLSSSEH 247

Query: 244 RVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQNY 303
           RVVL K  + RFS+AFF   ED +K I+AP+  EVVG+   R+Y+PF    Y  F+ Q+ 
Sbjct: 248 RVVL-KRIVNRFSLAFFWCFED-EKVIFAPN--EVVGEDNLRIYKPFVCADYLKFR-QSS 307

Query: 304 EKGK-----YLIKDIIGT 307
           EKG+     + +KD  GT
Sbjct: 308 EKGRFDKVGFTVKDFAGT 316

[Close]

BLAST of Cp4.1LG12g10390 vs. NCBI nr
Match: gi|297742515|emb|CBI34664.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 256.5 bits (654), Expect = 5.8e-65
Identity = 145/313 (46.33%), Postives = 205/313 (65.50%), Query Frame = 1


Query: 4   VEVPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLLKEFYQKLISGADQLFSSPEET 63
           +++PV+D+S Q +  S  +SL +AC E+GFF + NHG+ ++ + K+   +  +FS P ++
Sbjct: 8   IQLPVLDLS-QPVHPSFLTSLSQACEEWGFFHVMNHGIPQDLFSKVCLLSKHIFSLPADS 67

Query: 64  KQKL--TMGVSSYTPRFEVSPFFESFKVSGPNFSASAAVQGFTQSLFGHQATEFSNLLDE 123
           K KL  +  + +YTPRF  SPFFES +++GP+F ASA  +     LF  Q +EFS  L E
Sbjct: 68  KLKLGPSSSIKTYTPRFIASPFFESLRIAGPDFLASA--KSSVAELFDQQNSEFSETLQE 127

Query: 124 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSN--------GFTPRDMNEEEEEEVFK 183
           YGSKM E+SKR+V+ +L  LGD  E KF +SEF+N         ++P +  E+EE E   
Sbjct: 128 YGSKMVELSKRIVEAVLMSLGDGYERKFNESEFNNCQGYLRLVNYSPPECVEDEEVEGLG 187

Query: 184 QHTDLSYVTIVFQNDIRGLHMRLKGGDWMDVRPSEDDNLVMNIGDFMEAWSNGRLRSAVH 243
            HTD+S +TIV+Q++I GL MR KGG+W+D+ P E   LV+NIGD M+ WSNGRLRS+ H
Sbjct: 188 MHTDMSCITIVYQDEIGGLQMRSKGGEWIDITPCEG-TLVVNIGDLMQGWSNGRLRSSEH 247

Query: 244 RVVLRKNNMKRFSMAFFLTPEDNDKEIYAPSEVEVVGKGKSRLYRPFSPQQYRLFKEQN- 303
           RVVL K  + R S+AFF   ED +K + AP   E+VG+G SR+Y+PF   +Y  F+E N 
Sbjct: 248 RVVL-KRFVNRLSLAFFWCFED-EKVVLAPD--EIVGEGNSRIYKPFVCLEYLKFRESNE 307

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g10390 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GAOX2_ARATH	2.7e-22	30.07	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1	[more]
G2OX3_ARATH	2.5e-20	28.62	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA2OX3 PE=2 SV=1	[more]
AOP1C_ARATH	5.6e-20	26.96	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AO...	[more]
G3OX_PEA	5.6e-20	27.69	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1	[more]
AOP1V_ARATH	9.6e-20	26.55	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]

BLAST of Cp4.1LG12g10390 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KW97_CUCSA	1.9e-83	55.78	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G022900 PE=3 SV=1	[more]
B9R9V9_RICCO	8.1e-66	50.16	Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1500930 PE=3 SV=1	[more]
A0A061FZ75_THECC	3.1e-65	48.43	2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao GN=TCM_014890 PE=3 SV=1	[more]
D7TVY9_VITVI	4.0e-65	46.33	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03440 PE=3 SV=...	[more]
B9RDS1_RICCO	1.2e-64	46.52	Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1615250 PE=3 SV=1	[more]

BLAST of Cp4.1LG12g10390 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G23340.1	5.7e-63	45.31	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G51310.1	1.6e-57	43.04	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G51810.1	1.5e-23	30.07	gibberellin 20 oxidase 2	[more]
AT1G78550.1	3.8e-22	30.03	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT2G34555.1	1.4e-21	28.62	gibberellin 2-oxidase 3	[more]

BLAST of Cp4.1LG12g10390 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449458351\|ref\|XP_004146911.1\|	2.8e-83	55.78	PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus]	[more]
gi\|659107869\|ref\|XP_008453897.1\|	2.3e-82	55.78	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo]	[more]
gi\|255539839\|ref\|XP_002510984.1\|	1.2e-65	50.16	PREDICTED: gibberellin 2-beta-dioxygenase 7 [Ricinus communis]	[more]
gi\|590671448\|ref\|XP_007038334.1\|	4.4e-65	48.43	2-oxoglutarate-dependent dioxygenase [Theobroma cacao]	[more]
gi\|297742515\|emb\|CBI34664.3\|	5.8e-65	46.33	unnamed protein product [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR026992	DIOX_N
IPR005123	Oxoglu/Fe-dep_dioxygenase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g10390.1	Cp4.1LG12g10390.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 171..253 score: 3.8
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 156..253 score: 9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 6..69 score: 2.2
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 4..299 score: 2.1
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 4..299 score: 1.6
None	No IPR available	PANTHER	PTHR10209:SF151	SUBFAMILY NOT NAMED	coord: 4..299 score: 1.6
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 2..293 score: 1.17

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG12g10390	CmaCh17G013390	Cucurbita maxima (Rimu)	cmacpeB371
Cp4.1LG12g10390	CmoCh17G013040	Cucurbita moschata (Rifu)	cmocpeB335
Cp4.1LG12g10390	Cla014839	Watermelon (97103) v1	cpewmB135
Cp4.1LG12g10390	ClCG09G007850	Watermelon (Charleston Gray)	cpewcgB129
Cp4.1LG12g10390	Lsi02G022030	Bottle gourd (USVL1VR-Ls)	cpelsiB111
Cp4.1LG12g10390	Bhi09G000975	Wax gourd	cpewgoB0209

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG12g10390	Melon (DHL92) v3.6.1	cpemedB146
Cp4.1LG12g10390	Silver-seed gourd	carcpeB0018
Cp4.1LG12g10390	Silver-seed gourd	carcpeB0116
Cp4.1LG12g10390	Cucumber (Chinese Long) v3	cpecucB0182
Cp4.1LG12g10390	Cucurbita pepo (Zucchini)	cpecpeB173
Cp4.1LG12g10390	Cucumber (Gy14) v1	cgycpeB1027
Cp4.1LG12g10390	Cucurbita maxima (Rimu)	cmacpeB896
Cp4.1LG12g10390	Cucurbita moschata (Rifu)	cmocpeB834
Cp4.1LG12g10390	Wild cucumber (PI 183967)	cpecpiB159
Cp4.1LG12g10390	Cucumber (Chinese Long) v2	cpecuB157
Cp4.1LG12g10390	Melon (DHL92) v3.5.1	cpemeB120
Cp4.1LG12g10390	Cucumber (Gy14) v2	cgybcpeB900