Cp4.1LG12g10100 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG12g10100
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Cytochrome P450
Location	Cp4.1LG12 : 9683025 .. 9684893 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGGTTATTGAGTATTTCTTTAATTAAAAAAAAAAAAAAAATTAAGGATATTTTTGAAAAGTTAATTATTATTATTATTATTATTATAGAAAAAAAGAAGTTACTGTTAATTAATAATCTGCGTTGTTGTTATATAGAGTTGTTGGAGCAGGAACCCATGTCATCCACAATTAAGTGAGCATCCATGGAAACCAGCAGCTATGTTGCAGCGCTGATTACTTTCTTGGCTGTGGGTTCCATTTTTTTGTTGCAGAGAAGAAAAAACAGAGCCAAAAATCTCCCGCCGACTCCCTGGGCTCTGCCCATCCTGGGTCATCTTCATCTCCTCAAGCACCCAGTCCACAGAACTTTACAGTCCCTCGCCCAAAAACACGGCCCAATTTTCACTCTCCGCCTCGGCTCCCGTCTCGCCGTCGTTGTATCATCGGCACCAGCCGTCGAAGAATGCTTCACCACAAACGACATCGTTTTCGCTAACCGCCCTCAATTCGTTTCCGGCATGTACCTCAGCTACGGCAACACCACGATGGGGGCTGCCCCGTACGGCGATCACTGGCGCAATCTACGGCGCTTGAGCTCCACAGAAATCTTGTCGTCGATCCGCCTCAACATGTCGGCCGGTATCCGGAAGGAAGAAGTCAATATTTTACTCCGCAAATTGTATCAATTTTCGAGGCATGATTTTGGGAAAGTGAAATTGAAAACCCTGTTTTCGGAGCTGACTTTCAACATAATTATGAGAATGGTGGCCGGAAAACGGTATTACGGCGATGAAGTGACGGAATTGGAAGAGGCGAAAGAGTTCCGACAAATTATGGAGACGTCGTTTCGATTGGGGTCGTACCCTGGTGATTTCTTACCGTTCCTGAAGTGGGTTGATCGGCAATACCCCAAAAGGATGAAGAGTCTGGGCAGAGCCATTGATTCGTTGCTGCAGAATTTGGTGGATGAACACCGGAGATATCAAAATGAAGGGAAAAAGCTTGATACCATGATCAGCCATCTGCTCTGTTTGCAGGAGTCTCAGCCCGAGTATTACTCAGATGAAATTATCAAAGGCCTCATCGTTGTAAGTAAATTCTCACCCTTAAACTCATAATACTCTGTTTCTCTCAAGAACATGTTTTGATTTGTTGAATTTGCAGACAATTTTAACAGCAGCGACGGAAACATCGTCTGTGACGATAGAATGGGCAATGTCCAATCTACTCAACCATCCAGAGGCATTGAACAAGGCCAGGGCTGAAGTTGACGCACAGATTGGGCAAGATCGGTTACTGGAAGAAGAAGACATCCCCAATCTGCCATACCTCCAAAACGTGATCTCTGAGACACTGCGCTTGTACCCAGCAGCTCCACTCTTGGCGCCACATTTTTCATCCGAGAGTTGTTCTCTGGGGGGATACCATATTCCTGCTGGCACTACCTTGTTGGTGAATGCGTGGGCGGTGCAAAGGGATCCCGAAGTTTGGGAGGACCCGACAAGGTTTAAACCTGAGAGGTTTGAGAGCGGGGGAGTGGCTGGCAACGCTTACAAATTCTTGCCGTTTGGATTGGGGAGGAGGGCTTGTCCTGGGATGGGCATGGCGAACCGTGTGGTGGGCTTGGCTTTGGGGTCGCTGATTCAGAGCTTTGAGTGGAAGAGGGTGAGTGACGAGGAATTGGACATGACTGAAAGCCAAGGGATCTCTATGTATAAAGTTGAGCCATTGGAGGCCTTATGTAGAGCTCGCCCCATCATCAAAAACCTTGCATTTCATTGATACCTCTACCTAGGAATGCCCTTTAGATTATTATTAGTTTTTTTCTTTTTTTTTTTTTAATGTCAGCTGCACTTTGATGTCGCGAATAAATCGAGATTTTGA

mRNA sequence

ATGAATGAGAAGAAAAAACAGAGCCAAAAATCTCCCGCCGACTCCCTGGGCTCTGCCCATCCTGGGTCATCTTCATCTCCTCAAGCACCCATGAAATTGAAAACCCTGTTTTCGGAGCTGACTTTCAACATAATTATGAGAATGGTGGCCGGAAAACGGTATTACGGCGATGAAGTGACGGAATTGGAAGAGGCGAAAGAGTTCCGACAAATTATGGAGACGTCGTTTCGATTGGGGTCGTACCCTGGTGATTTCTTACCGTTCCTGAAGTGGGTTGATCGGCAATACCCCAAAAGGATGAAGAGTCTGGGCAGAGCCATTGATTCGTTGCTGCAGAATTTGGTGGATGAACACCGGAGATATCAAAATGAAGGGAAAAAGCTTGATACCATGATCAGCCATCTGCTCTGTTTGCAGGAGTCTCAGCCCGAGTATTACTCAGATGAAATTATCAAAGGCCTCATCGTTCTGCACTTTGATGTCGCGAATAAATCGAGATTTTGA

Coding sequence (CDS)

ATGAATGAGAAGAAAAAACAGAGCCAAAAATCTCCCGCCGACTCCCTGGGCTCTGCCCATCCTGGGTCATCTTCATCTCCTCAAGCACCCATGAAATTGAAAACCCTGTTTTCGGAGCTGACTTTCAACATAATTATGAGAATGGTGGCCGGAAAACGGTATTACGGCGATGAAGTGACGGAATTGGAAGAGGCGAAAGAGTTCCGACAAATTATGGAGACGTCGTTTCGATTGGGGTCGTACCCTGGTGATTTCTTACCGTTCCTGAAGTGGGTTGATCGGCAATACCCCAAAAGGATGAAGAGTCTGGGCAGAGCCATTGATTCGTTGCTGCAGAATTTGGTGGATGAACACCGGAGATATCAAAATGAAGGGAAAAAGCTTGATACCATGATCAGCCATCTGCTCTGTTTGCAGGAGTCTCAGCCCGAGTATTACTCAGATGAAATTATCAAAGGCCTCATCGTTCTGCACTTTGATGTCGCGAATAAATCGAGATTTTGA

Protein sequence

MNEKKKQSQKSPADSLGSAHPGSSSSPQAPMKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPGDFLPFLKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSDEIIKGLIVLHFDVANKSRF

Homology

[Close]

BLAST of Cp4.1LG12g10100 vs. Swiss-Prot
Match: C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.9e-28
Identity = 66/132 (50.00%), Postives = 94/132 (71.21%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDE--VTELEEAKEFRQIMETSFRLG--SYPGDFL 90
           ++L+ +FSE+TFN IMRMV+GKRYYG++  V+++EEA+ FR I++    LG  +  GDFL
Sbjct: 170 VELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFL 229

Query: 91  PFLKWVDRQ-YPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEY 150
            FL+W D     KR+K + +  D+ LQ L+DEHR  +      +TMI HLL  Q+SQPEY
Sbjct: 230 GFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFGKRNS---NTMIDHLLTQQQSQPEY 289

Query: 151 YSDEIIKGLIVL 158
           Y+D+IIKGL+V+
Sbjct: 290 YTDQIIKGLMVV 298

[Close]

BLAST of Cp4.1LG12g10100 vs. Swiss-Prot
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 1.1e-25
Identity = 57/128 (44.53%), Postives = 91/128 (71.09%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIME--TSFRLGSYPGDFLPF 90
           ++L+ L S+LTFN I+RMV GKRYYGD+V   EEA+ F++++     +   ++  D+LP 
Sbjct: 172 IELEPLLSDLTFNNIVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPI 231

Query: 91  LKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSD 150
           LK    ++ K +K++G+++D +LQ L+DE RR     K+ +TM++HL+ LQ+ QPEYY+D
Sbjct: 232 LKLFGNKFEKEVKAIGKSMDDILQRLLDECRR----DKEGNTMVNHLISLQQQQPEYYTD 291

Query: 151 EIIKGLIV 157
            IIKGL++
Sbjct: 292 VIIKGLMM 295

[Close]

BLAST of Cp4.1LG12g10100 vs. Swiss-Prot
Match: C81F3_ARATH (Cytochrome P450 81F3 OS=Arabidopsis thaliana GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.2e-25
Identity = 58/128 (45.31%), Postives = 88/128 (68.75%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIME--TSFRLGSYPGDFLPF 90
           ++L+ L S+LTFN I+RMV GKRYYGDEV   EEA  F++++          +PGD+LPF
Sbjct: 167 IELEPLLSDLTFNNIVRMVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPF 226

Query: 91  LKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSD 150
           +K     + K++K+L  A+D +LQ L++E +R     K  +TM++HLL LQ+++PEYY+D
Sbjct: 227 MKMFGGSFEKKVKALAEAMDEILQRLLEECKR----DKDGNTMVNHLLSLQQNEPEYYTD 286

Query: 151 EIIKGLIV 157
             IKGL++
Sbjct: 287 VTIKGLML 290

[Close]

BLAST of Cp4.1LG12g10100 vs. Swiss-Prot
Match: C81F2_ARATH (Cytochrome P450 81F2 OS=Arabidopsis thaliana GN=CYP81F2 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.2e-25
Identity = 62/128 (48.44%), Postives = 90/128 (70.31%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIM-ETSFRLG-SYPGDFLPF 90
           ++L+ L ++LTFN I+RMV G+RYYGD+V   EEA  F++++ + +   G S+PGD+LP 
Sbjct: 167 VELEPLLADLTFNNIVRMVTGRRYYGDQVHNKEEANLFKKLVTDINDNSGASHPGDYLPI 226

Query: 91  LKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSD 150
           LK     Y K++K+LG A+D+ LQ L+DE R       + +TM+SHLL LQ  QP+YYSD
Sbjct: 227 LKVFGHGYEKKVKALGEAMDAFLQRLLDECR----INGESNTMVSHLLSLQLDQPKYYSD 286

Query: 151 EIIKGLIV 157
            IIKGL++
Sbjct: 287 VIIKGLML 290

[Close]

BLAST of Cp4.1LG12g10100 vs. Swiss-Prot
Match: C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 5.4e-25
Identity = 63/130 (48.46%), Postives = 88/130 (67.69%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDE--VTELEEAKEFRQIMETSFRL--GSYPGDFL 90
           ++L+   +E+TFN +MRM++GKRYYGD+  V+++EEAK+FR+I+     L   +  GDFL
Sbjct: 171 VELRPRLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFL 230

Query: 91  PFLKWVDRQ-YPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEY 150
           P L+ VD     KR K + +  ++ L+ L++EHRR  N      TMI HLL L ESQPEY
Sbjct: 231 PLLRVVDLDNLEKRCKRIAKRSNAFLEGLIEEHRR-GNIHSDGGTMIDHLLKLSESQPEY 290

Query: 151 YSDEIIKGLI 156
           YSD +IKGLI
Sbjct: 291 YSDHLIKGLI 299

[Close]

BLAST of Cp4.1LG12g10100 vs. TrEMBL
Match: A0A0A0K9E5_CUCSA (Cytochrome P450 OS=Cucumis sativus GN=Csa6G088180 PE=2 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 8.9e-51
Identity = 98/135 (72.59%), Postives = 119/135 (88.15%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPGDFLPFLK 90
           +KLK+LFSELTFNIIMRM+AGKRYYG+EV+ELEEAK+FR+IME +F+LGSYPGDFLPFLK
Sbjct: 169 VKLKSLFSELTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLK 228

Query: 91  WVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSDEI 150
           WVD QY KR++SLG+  D  LQNLVDEHRR + EG+K +TMISHLLCLQ++QP+YY+DEI
Sbjct: 229 WVDWQYTKRVESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEI 288

Query: 151 IKGLIVLHFDVANKS 166
           IKGLIV     A ++
Sbjct: 289 IKGLIVTILSAATET 303

[Close]

BLAST of Cp4.1LG12g10100 vs. TrEMBL
Match: A0A061DI90_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 5, putative OS=Theobroma cacao GN=TCM_000715 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.0e-35
Identity = 79/132 (59.85%), Postives = 100/132 (75.76%), Query Frame = 1


Query: 29  APMKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRL--GSYPGDFL 88
           A ++LKTLF+ELTFNIIMR+VAGKRYYGDEV   EEA+ FR ++E  F L   SYPGDFL
Sbjct: 196 AKVELKTLFAELTFNIIMRIVAGKRYYGDEVRGSEEARGFRAMIEELFELTVSSYPGDFL 255

Query: 89  PFLKWVDRQ-YPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEY 148
           P L WVD   Y K++K + +  D+  Q L+DEHRR +   +  +TMISHLL LQ+SQPEY
Sbjct: 256 PLLSWVDYNGYIKKVKLVAKNADAFTQGLIDEHRRNKGNLELKNTMISHLLTLQKSQPEY 315

Query: 149 YSDEIIKGLIVL 158
           Y+DEIIKGL+++
Sbjct: 316 YTDEIIKGLVLV 327

[Close]

BLAST of Cp4.1LG12g10100 vs. TrEMBL
Match: M5W5F9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004635mg PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.6e-34
Identity = 80/133 (60.15%), Postives = 102/133 (76.69%), Query Frame = 1


Query: 29  APMKLKTLFSELTFNIIMRMVAGKRYYGDEVT-ELEEAKEFRQIMETSFRLGSY--PGDF 88
           A ++LK++F+ELTFNIIM MVAGKRYYGD+++ + EEAK+FRQIM  +F  GS   P DF
Sbjct: 169 AKVELKSMFNELTFNIIMTMVAGKRYYGDDMSVDKEEAKQFRQIMSDAFLYGSAANPADF 228

Query: 89  LPFLKWVDRQ-YPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPE 148
           LP L WV R  Y K++K+L +  D+ LQ L+DEHRR   +GK   TMI HLL LQESQPE
Sbjct: 229 LPILNWVGRGGYEKKVKTLAKRTDAFLQGLIDEHRR---KGKNGTTMIDHLLSLQESQPE 288

Query: 149 YYSDEIIKGLIVL 158
           YY+D+IIKGLI++
Sbjct: 289 YYNDQIIKGLILV 298

[Close]

BLAST of Cp4.1LG12g10100 vs. TrEMBL
Match: A0A0D2QME7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G027500 PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 9.9e-34
Identity = 76/132 (57.58%), Postives = 99/132 (75.00%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYG--DEVTELEEAKEFRQIMETSFRLG--SYPGDFL 90
           +++K++FSEL FNI+MR+VAGKRYYG  DE + +EEAKEFR+++E    L   SYPGDFL
Sbjct: 173 VEMKSMFSELVFNIVMRLVAGKRYYGGGDEASGIEEAKEFRELIEELCELAVSSYPGDFL 232

Query: 91  PFLKWVDRQ-YPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEY 150
           P L+W+D   + KR+K  G   D  LQ LVDEHR  ++  KK +TM+SHLL LQES+PEY
Sbjct: 233 PVLQWIDYNGHIKRLKKAGSKADKFLQGLVDEHRSNKDAFKKKNTMLSHLLTLQESEPEY 292

Query: 151 YSDEIIKGLIVL 158
           YSDE IK LI++
Sbjct: 293 YSDETIKALILV 304

[Close]

BLAST of Cp4.1LG12g10100 vs. TrEMBL
Match: A0A061DGU7_THECC (Cytochrome P450, family 81, subfamily H, polypeptide 1, putative OS=Theobroma cacao GN=TCM_000714 PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.1e-32
Identity = 76/136 (55.88%), Postives = 102/136 (75.00%), Query Frame = 1


Query: 24  SSSPQAPMKLKTLFSELTFNIIMRMVAGKRYYGDEVT-ELEEAKEFRQIMETSFRLG--S 83
           S++  A ++LK+LFSELTFNIIMRM+AGKRY+GD+V+   EE + FR+I++  F L   S
Sbjct: 160 SANGFAKVELKSLFSELTFNIIMRMIAGKRYFGDDVSGNYEEGRRFREIIKERFELAVSS 219

Query: 84  YPGDFLPFLKWVDRQ-YPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQ 143
           YPGDF+P L+ VD   Y  R+  LG   D L+Q ++DEHRR + + K  +TMI+HLL LQ
Sbjct: 220 YPGDFVPILQLVDYNGYISRITRLGNKADELMQGMIDEHRRNKGDLKIKNTMITHLLSLQ 279

Query: 144 ESQPEYYSDEIIKGLI 156
           ESQP+YY+DEIIKGL+
Sbjct: 280 ESQPDYYTDEIIKGLV 295

[Close]

BLAST of Cp4.1LG12g10100 vs. TAIR10
Match: AT4G37430.1 (AT4G37430.1 cytochrome P450, family 91, subfamily A, polypeptide 2)

HSP 1 Score: 117.9 bits (294), Expect = 6.2e-27
Identity = 57/128 (44.53%), Postives = 91/128 (71.09%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIME--TSFRLGSYPGDFLPF 90
           ++L+ L S+LTFN I+RMV GKRYYGD+V   EEA+ F++++     +   ++  D+LP 
Sbjct: 172 IELEPLLSDLTFNNIVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPI 231

Query: 91  LKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSD 150
           LK    ++ K +K++G+++D +LQ L+DE RR     K+ +TM++HL+ LQ+ QPEYY+D
Sbjct: 232 LKLFGNKFEKEVKAIGKSMDDILQRLLDECRR----DKEGNTMVNHLISLQQQQPEYYTD 291

Query: 151 EIIKGLIV 157
            IIKGL++
Sbjct: 292 VIIKGLMM 295

[Close]

BLAST of Cp4.1LG12g10100 vs. TAIR10
Match: AT4G37400.1 (AT4G37400.1 cytochrome P450, family 81, subfamily F, polypeptide 3)

HSP 1 Score: 116.3 bits (290), Expect = 1.8e-26
Identity = 58/128 (45.31%), Postives = 88/128 (68.75%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIME--TSFRLGSYPGDFLPF 90
           ++L+ L S+LTFN I+RMV GKRYYGDEV   EEA  F++++          +PGD+LPF
Sbjct: 167 IELEPLLSDLTFNNIVRMVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPF 226

Query: 91  LKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSD 150
           +K     + K++K+L  A+D +LQ L++E +R     K  +TM++HLL LQ+++PEYY+D
Sbjct: 227 MKMFGGSFEKKVKALAEAMDEILQRLLEECKR----DKDGNTMVNHLLSLQQNEPEYYTD 286

Query: 151 EIIKGLIV 157
             IKGL++
Sbjct: 287 VTIKGLML 290

[Close]

BLAST of Cp4.1LG12g10100 vs. TAIR10
Match: AT5G57220.1 (AT5G57220.1 cytochrome P450, family 81, subfamily F, polypeptide 2)

HSP 1 Score: 116.3 bits (290), Expect = 1.8e-26
Identity = 62/128 (48.44%), Postives = 90/128 (70.31%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIM-ETSFRLG-SYPGDFLPF 90
           ++L+ L ++LTFN I+RMV G+RYYGD+V   EEA  F++++ + +   G S+PGD+LP 
Sbjct: 167 VELEPLLADLTFNNIVRMVTGRRYYGDQVHNKEEANLFKKLVTDINDNSGASHPGDYLPI 226

Query: 91  LKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSD 150
           LK     Y K++K+LG A+D+ LQ L+DE R       + +TM+SHLL LQ  QP+YYSD
Sbjct: 227 LKVFGHGYEKKVKALGEAMDAFLQRLLDECR----INGESNTMVSHLLSLQLDQPKYYSD 286

Query: 151 EIIKGLIV 157
            IIKGL++
Sbjct: 287 VIIKGLML 290

[Close]

BLAST of Cp4.1LG12g10100 vs. TAIR10
Match: AT4G37340.1 (AT4G37340.1 cytochrome P450, family 81, subfamily D, polypeptide 3)

HSP 1 Score: 114.4 bits (285), Expect = 6.8e-26
Identity = 61/137 (44.53%), Postives = 89/137 (64.96%), Query Frame = 1


Query: 23  SSSSPQAPMKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETS---FRLG 82
           +SS     +++K++FS LTFN I+RM+AGK YYGD   +  EAK  R+++      F  G
Sbjct: 163 NSSLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAG 222

Query: 83  SYPGDFLPFLKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQ 142
           +   D+LP L W+     KR+K +   +D  LQ LVDE R  + + K+ +TM+ HLLCLQ
Sbjct: 223 N-TADYLPILTWITGS-EKRIKKIASRLDEFLQGLVDERR--EGKEKRQNTMVDHLLCLQ 282

Query: 143 ESQPEYYSDEIIKGLIV 157
           E+QPEYY+D IIKG+++
Sbjct: 283 ETQPEYYTDNIIKGIML 295

[Close]

BLAST of Cp4.1LG12g10100 vs. TAIR10
Match: AT4G37360.1 (AT4G37360.1 cytochrome P450, family 81, subfamily D, polypeptide 2)

HSP 1 Score: 110.5 bits (275), Expect = 9.8e-25
Identity = 62/137 (45.26%), Postives = 87/137 (63.50%), Query Frame = 1


Query: 23  SSSSPQAPMKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYP 82
           +SS     +++K++FS+LTFN I+RM+AGK YYGD   +  EAK  R ++  +    S P
Sbjct: 163 NSSYGFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMS-SSGP 222

Query: 83  G---DFLPFLKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQ 142
           G   D++P L W+      R+K L   +D  LQ LVDE R  + + KK +TM+ HLLCLQ
Sbjct: 223 GNAADYIPILTWITYS-ETRIKKLAGRLDEFLQGLVDEKR--EGKEKKENTMVDHLLCLQ 282

Query: 143 ESQPEYYSDEIIKGLIV 157
           E+QPEYY D IIKG ++
Sbjct: 283 ETQPEYYMDRIIKGTML 295

[Close]

BLAST of Cp4.1LG12g10100 vs. NCBI nr
Match: gi|449445804|ref|XP_004140662.1| (PREDICTED: cytochrome P450 81E8 [Cucumis sativus])

HSP 1 Score: 208.0 bits (528), Expect = 1.3e-50
Identity = 98/135 (72.59%), Postives = 119/135 (88.15%), Query Frame = 1


Query: 31  MKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPGDFLPFLK 90
           +KLK+LFSELTFNIIMRM+AGKRYYG+EV+ELEEAK+FR+IME +F+LGSYPGDFLPFLK
Sbjct: 169 VKLKSLFSELTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLK 228

Query: 91  WVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSDEI 150
           WVD QY KR++SLG+  D  LQNLVDEHRR + EG+K +TMISHLLCLQ++QP+YY+DEI
Sbjct: 229 WVDWQYTKRVESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEI 288

Query: 151 IKGLIVLHFDVANKS 166
           IKGLIV     A ++
Sbjct: 289 IKGLIVTILSAATET 303

[Close]

BLAST of Cp4.1LG12g10100 vs. NCBI nr
Match: gi|659119967|ref|XP_008459939.1| (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 198.7 bits (504), Expect = 7.8e-48
Identity = 96/142 (67.61%), Postives = 119/142 (83.80%), Query Frame = 1


Query: 24  SSSPQAPMKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPG 83
           SS+    +KLK+LFSELTFN+IM MVAGKRYYG+EV+ELEEAK+FR+IME +F+LGSYPG
Sbjct: 165 SSTDFGKVKLKSLFSELTFNMIMTMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPG 224

Query: 84  DFLPFLKWVDRQYPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQP 143
           DFLPFLKWVD QY KR++SLG+  D  LQNLVDEHR  + EG+K +TM+SHLL LQ++QP
Sbjct: 225 DFLPFLKWVDWQYTKRVESLGKDTDWFLQNLVDEHRSNEEEGEKGNTMVSHLLRLQKTQP 284

Query: 144 EYYSDEIIKGLIVLHFDVANKS 166
           +YY+DEIIKGLIV     A ++
Sbjct: 285 DYYTDEIIKGLIVTILSAATET 306

[Close]

BLAST of Cp4.1LG12g10100 vs. NCBI nr
Match: gi|645220091|ref|XP_008238686.1| (PREDICTED: cytochrome P450 81D11-like [Prunus mume])

HSP 1 Score: 156.8 bits (395), Expect = 3.4e-35
Identity = 82/133 (61.65%), Postives = 103/133 (77.44%), Query Frame = 1


Query: 29  APMKLKTLFSELTFNIIMRMVAGKRYYGDEVT-ELEEAKEFRQIMETSFRLGSY--PGDF 88
           A ++LK++F+ELTFNIIM MVAGKRYYGD+V+ + EEAK+FRQIM  +F  GS   P DF
Sbjct: 169 AKVELKSMFNELTFNIIMTMVAGKRYYGDDVSVDKEEAKQFRQIMSDAFLYGSAANPADF 228

Query: 89  LPFLKWVDRQ-YPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPE 148
           LP L WV R  Y K++K+LG+  D+ LQ L+DEHRR   +GK   TMI HLL LQESQPE
Sbjct: 229 LPILNWVGRGGYEKKVKTLGKRTDAFLQGLIDEHRR---KGKNGTTMIDHLLSLQESQPE 288

Query: 149 YYSDEIIKGLIVL 158
           YY+D+IIKGLI++
Sbjct: 289 YYNDQIIKGLILV 298

[Close]

BLAST of Cp4.1LG12g10100 vs. NCBI nr
Match: gi|590705372|ref|XP_007047419.1| (Cytochrome P450, family 81, subfamily D, polypeptide 5, putative [Theobroma cacao])

HSP 1 Score: 156.0 bits (393), Expect = 5.8e-35
Identity = 79/132 (59.85%), Postives = 100/132 (75.76%), Query Frame = 1


Query: 29  APMKLKTLFSELTFNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRL--GSYPGDFL 88
           A ++LKTLF+ELTFNIIMR+VAGKRYYGDEV   EEA+ FR ++E  F L   SYPGDFL
Sbjct: 196 AKVELKTLFAELTFNIIMRIVAGKRYYGDEVRGSEEARGFRAMIEELFELTVSSYPGDFL 255

Query: 89  PFLKWVDRQ-YPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEY 148
           P L WVD   Y K++K + +  D+  Q L+DEHRR +   +  +TMISHLL LQ+SQPEY
Sbjct: 256 PLLSWVDYNGYIKKVKLVAKNADAFTQGLIDEHRRNKGNLELKNTMISHLLTLQKSQPEY 315

Query: 149 YSDEIIKGLIVL 158
           Y+DEIIKGL+++
Sbjct: 316 YTDEIIKGLVLV 327

[Close]

BLAST of Cp4.1LG12g10100 vs. NCBI nr
Match: gi|595837015|ref|XP_007207372.1| (hypothetical protein PRUPE_ppa004635mg [Prunus persica])

HSP 1 Score: 153.3 bits (386), Expect = 3.8e-34
Identity = 80/133 (60.15%), Postives = 102/133 (76.69%), Query Frame = 1


Query: 29  APMKLKTLFSELTFNIIMRMVAGKRYYGDEVT-ELEEAKEFRQIMETSFRLGSY--PGDF 88
           A ++LK++F+ELTFNIIM MVAGKRYYGD+++ + EEAK+FRQIM  +F  GS   P DF
Sbjct: 169 AKVELKSMFNELTFNIIMTMVAGKRYYGDDMSVDKEEAKQFRQIMSDAFLYGSAANPADF 228

Query: 89  LPFLKWVDRQ-YPKRMKSLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPE 148
           LP L WV R  Y K++K+L +  D+ LQ L+DEHRR   +GK   TMI HLL LQESQPE
Sbjct: 229 LPILNWVGRGGYEKKVKTLAKRTDAFLQGLIDEHRR---KGKNGTTMIDHLLSLQESQPE 288

Query: 149 YYSDEIIKGLIVL 158
           YY+D+IIKGLI++
Sbjct: 289 YYNDQIIKGLILV 298

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g10100 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C81E8_MEDTR	8.9e-28	50.00	Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1	[more]
C81F1_ARATH	1.1e-25	44.53	Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2	[more]
C81F3_ARATH	3.2e-25	45.31	Cytochrome P450 81F3 OS=Arabidopsis thaliana GN=CYP81F3 PE=2 SV=1	[more]
C81F2_ARATH	3.2e-25	48.44	Cytochrome P450 81F2 OS=Arabidopsis thaliana GN=CYP81F2 PE=2 SV=1	[more]
C81E9_MEDTR	5.4e-25	48.46	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1	[more]

BLAST of Cp4.1LG12g10100 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K9E5_CUCSA	8.9e-51	72.59	Cytochrome P450 OS=Cucumis sativus GN=Csa6G088180 PE=2 SV=1	[more]
A0A061DI90_THECC	4.0e-35	59.85	Cytochrome P450, family 81, subfamily D, polypeptide 5, putative OS=Theobroma ca...	[more]
M5W5F9_PRUPE	2.6e-34	60.15	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004635mg PE=3 SV=1	[more]
A0A0D2QME7_GOSRA	9.9e-34	57.58	Uncharacterized protein OS=Gossypium raimondii GN=B456_003G027500 PE=3 SV=1	[more]
A0A061DGU7_THECC	1.1e-32	55.88	Cytochrome P450, family 81, subfamily H, polypeptide 1, putative OS=Theobroma ca...	[more]

BLAST of Cp4.1LG12g10100 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G37430.1	6.2e-27	44.53	cytochrome P450, family 91, subfamily A, polypeptide 2	[more]
AT4G37400.1	1.8e-26	45.31	cytochrome P450, family 81, subfamily F, polypeptide 3	[more]
AT5G57220.1	1.8e-26	48.44	cytochrome P450, family 81, subfamily F, polypeptide 2	[more]
AT4G37340.1	6.8e-26	44.53	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT4G37360.1	9.8e-25	45.26	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]

BLAST of Cp4.1LG12g10100 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449445804\|ref\|XP_004140662.1\|	1.3e-50	72.59	PREDICTED: cytochrome P450 81E8 [Cucumis sativus]	[more]
gi\|659119967\|ref\|XP_008459939.1\|	7.8e-48	67.61	PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo]	[more]
gi\|645220091\|ref\|XP_008238686.1\|	3.4e-35	61.65	PREDICTED: cytochrome P450 81D11-like [Prunus mume]	[more]
gi\|590705372\|ref\|XP_007047419.1\|	5.8e-35	59.85	Cytochrome P450, family 81, subfamily D, polypeptide 5, putative [Theobroma caca...	[more]
gi\|595837015\|ref\|XP_007207372.1\|	3.8e-34	60.15	hypothetical protein PRUPE_ppa004635mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g10100.1	Cp4.1LG12g10100.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 27..158 score: 4.0
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 28..152 score: 5.
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 27..159 score: 1.06
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 29..155 score: 1.5
None	No IPR available	PANTHER	PTHR24298:SF116	SUBFAMILY NOT NAMED	coord: 29..155 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG12g10100	Cucsa.349040	Cucumber (Gy14) v1	cgycpeB0907
Cp4.1LG12g10100	CmoCh17G013400	Cucurbita moschata (Rifu)	cmocpeB335
Cp4.1LG12g10100	Csa6G088180	Cucumber (Chinese Long) v2	cpecuB142
Cp4.1LG12g10100	CSPI06G07160	Wild cucumber (PI 183967)	cpecpiB144
Cp4.1LG12g10100	MELO3C022375.2	Melon (DHL92) v3.6.1	cpemedB140
Cp4.1LG12g10100	CsaV3_6G007560	Cucumber (Chinese Long) v3	cpecucB0166
Cp4.1LG12g10100	Bhi12G002101	Wax gourd	cpewgoB0175
Cp4.1LG12g10100	CsGy6G007170	Cucumber (Gy14) v2	cgybcpeB722
Cp4.1LG12g10100	Carg03795	Silver-seed gourd	carcpeB1355
Cp4.1LG12g10100	Carg11551	Silver-seed gourd	carcpeB0018

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG12g10100	Cp4.1LG17g09460	Cucurbita pepo (Zucchini)	cpecpeB170

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG12g10100	Cucurbita maxima (Rimu)	cmacpeB371
Cp4.1LG12g10100	Cucurbita maxima (Rimu)	cmacpeB905
Cp4.1LG12g10100	Cucurbita moschata (Rifu)	cmocpeB841
Cp4.1LG12g10100	Bottle gourd (USVL1VR-Ls)	cpelsiB103
Cp4.1LG12g10100	Watermelon (Charleston Gray)	cpewcgB140
Cp4.1LG12g10100	Watermelon (97103) v1	cpewmB156
Cp4.1LG12g10100	Melon (DHL92) v3.5.1	cpemeB114