Cp4.1LG12g06210 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG12g06210 vs. Swiss-Prot
Match: STSYN_PEA (Stachyose synthase OS=Pisum sativum GN=STS1 PE=1 SV=1)

HSP 1 Score: 1221.1 bits (3158), Expect = 0.0e+00
Identity = 567/847 (66.94%), Postives = 693/847 (81.82%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSSD- 60
           MAPP ++ T  S+++K++S+    DLS+ K  V G PL  DVP NV F  FSSIC+ S+ 
Sbjct: 1   MAPPLNSTT--SNLIKTESI---FDLSERKFKVKGFPLFHDVPENVSFRSFSSICKPSES 60

Query: 61  -APLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGN 120
            AP  LLQ+V   SHKGGF GF    PSDRLMNS+G F G++F+S+FRFKTWWST W+G 
Sbjct: 61  NAPPSLLQKVLAYSHKGGFFGFSHETPSDRLMNSIGSFNGKDFLSIFRFKTWWSTQWIGK 120

Query: 121 SGSDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASS 180
           SGSDLQMETQW+++ VPE  SYV+IIPIIE  FRSAL PG +  V I AESGST VK S+
Sbjct: 121 SGSDLQMETQWILIEVPETKSYVVIIPIIEKCFRSALFPGFNDHVKIIAESGSTKVKEST 180

Query: 181 FDAIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDP 240
           F++IAYVH S+NPY LMKEAY+A RVHLN+FRLLEEK + +LVDKFGWCTWDAFYLTV+P
Sbjct: 181 FNSIAYVHFSENPYDLMKEAYSAIRVHLNSFRLLEEKTIPNLVDKFGWCTWDAFYLTVNP 240

Query: 241 VGIWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEEC 300
           +GI++G++DF +GG+ PRF+IIDDGWQSI+ DG DP  DAKNLVLGG QM+ RL+RF+EC
Sbjct: 241 IGIFHGLDDFSKGGVEPRFVIIDDGWQSISFDGYDPNEDAKNLVLGGEQMSGRLHRFDEC 300

Query: 301 ERFRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQ 360
            +FRKY+ G LLGPN+P +DP     LI K IE +++ + R++AI S  +D++E E+KI+
Sbjct: 301 YKFRKYESGLLLGPNSPPYDPNNFTDLILKGIEHEKLRKKREEAISSKSSDLAEIESKIK 360

Query: 361 KLKAELNEIFGNQEEETSSSKDDDDDSGLKAFTKDLRTKFKGLDDVFVWHALAGAWGGVR 420
           K+  E++++FG ++  +    +   + GLKAFTKDLRTKFKGLDDV+VWHAL GAWGGVR
Sbjct: 361 KVVKEIDDLFGGEQFSSGEKSEMKSEYGLKAFTKDLRTKFKGLDDVYVWHALCGAWGGVR 420

Query: 421 PGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGI 480
           P +THL++KI+PCKLSPGLDGTMEDLAVV+I + S+GLVHP QA++ +DSMHSYL++ GI
Sbjct: 421 PETTHLDTKIVPCKLSPGLDGTMEDLAVVEISKASLGLVHPSQANELYDSMHSYLAESGI 480

Query: 481 TGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYLGT 540
           TGVKVDVIH+LEYV +EYGGRVDLAK YY+GLT S+VKNF G G+ +SMQ CNDFF+LGT
Sbjct: 481 TGVKVDVIHSLEYVCDEYGGRVDLAKVYYEGLTKSIVKNFNGNGMIASMQHCNDFFFLGT 540

Query: 541 KQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCA 600
           KQ S+GRVGDDFWFQDP GDPMG +WLQGVHMIHC+YNS+WMGQ+IQPDWDMFQSDH+CA
Sbjct: 541 KQISMGRVGDDFWFQDPNGDPMGSFWLQGVHMIHCSYNSLWMGQMIQPDWDMFQSDHVCA 600

Query: 601 KFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKNPLFDN 660
           KFHAGSRAICGGPIYVSD+VG HDFDL+K+LV+PDGTIP+C YF LPTRDCLFKNPLFD+
Sbjct: 601 KFHAGSRAICGGPIYVSDNVGSHDFDLIKKLVFPDGTIPKCIYFPLPTRDCLFKNPLFDH 660

Query: 661 KTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEWDQKPET 720
            TVLKIWN NKYGGVIGAFNCQGAGWDP  Q+ +G PECYKP+  T+HV  VEWDQK ET
Sbjct: 661 TTVLKIWNFNKYGGVIGAFNCQGAGWDPIMQKFRGFPECYKPIPGTVHVTEVEWDQKEET 720

Query: 721 APMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIKFAPIGL 780
           + +G   EYVVYLNQA+++   T KSEP++ T++PSTFEL++F+P+ KL   IKFAPIGL
Sbjct: 721 SHLGKAEEYVVYLNQAEELSLMTLKSEPIQFTIQPSTFELYSFVPVTKLCGGIKFAPIGL 780

Query: 781 TNMFNSSGTIQHLKYNEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEWSSDGKLSF 840
           TNMFNS GT+  L+Y   G ++KVKG G+FLAYSS SPKK   NG EV+FEW  DGKL  
Sbjct: 781 TNMFNSGGTVIDLEYVGNGAKIKVKGGGSFLAYSSESPKKFQLNGCEVDFEWLGDGKLCV 840

Query: 841 DLPWIEE 846
           ++PWIEE
Sbjct: 841 NVPWIEE 842

BLAST of Cp4.1LG12g06210 vs. Swiss-Prot
Match: RFS4_ARATH (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana GN=RFS4 PE=2 SV=3)

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 515/847 (60.80%), Postives = 654/847 (77.21%), Query Frame = 1

Query: 861  NLIDVSDGKLNVK-GVPLLSDVPTNVFFSPFSSICQSSDAPLPLLQRVHTLSHKGGFLGF 920
            N  ++S+G L  K   P+L DVP NV F+PFSS   S+DAPLP+L RV   +HKGGFLGF
Sbjct: 32   NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 921  HQSNPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVILNVPEINSY 980
             + +PSDRLTNSLG+F+ REF+S+FRFK WWST W+G SGSDLQ ETQWV+L +PEI+SY
Sbjct: 92   TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEIDSY 151

Query: 981  VVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFDAIAYVHVSDNPYNLMKEAYA 1040
            V IIP IEG+FR++L PG  G VLICAESGST VK SSF +IAY+H+ DNPYNLMKEA++
Sbjct: 152  VAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFS 211

Query: 1041 AIRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPAGIWSGLNEFVEGGISPRFLII 1100
            A+RVH+NTF+LLEEK +  +VDKFGWCTWDA YLTVDPA IW+G+ EF +GG+ P+F+II
Sbjct: 212  ALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVII 271

Query: 1101 DDGWQSINMDGDDPTRDTKNLVWGGTQMTSRLYSFEECEKFRKYKGGCLLEPNAPSFDPM 1160
            DDGWQSIN DGD+  +D +NLV GG QMT+RL SF+EC+KFR YKGG  +  +A  F+P+
Sbjct: 272  DDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPL 331

Query: 1161 KPKLLMNKAIE-IDEVGKNRDKAIRSGITDVSQFETKIQKLKEELNEIFERYEEQSSATS 1220
            KPK+L+ KA E I  +   R     SG  D+++ + KI+ L EELN +F+  E++ S  S
Sbjct: 332  KPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLGS 391

Query: 1221 KDGDEFGLKAFTRDLRTRFKGLDDVFVWHALAGAWGGVRPGS-THLNSKLTPCKLSPGLD 1280
             D    G+ AFT+DLR RFK LDD++VWHAL GAW GVRP +   L +K+ P +LSP L 
Sbjct: 392  DDVSGSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAPFELSPSLG 451

Query: 1281 GSMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGVTGVKVDVMHTLEYVSEEYGG 1340
             +M DLAV K++E  IGLVHP +A +F+DSMHSYL+ VGVTG K+DV  TLE ++EE+GG
Sbjct: 452  ATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQTLESLAEEHGG 511

Query: 1341 RVDLAKAYYNGLSNSLLKNFKGTGLFSSMQQCNDFFYLGTKQISIGRVGDDVWVQDPQGD 1400
            RV+LAKAYY+GL+ S++KNF GT + +SMQQCN+FF+L TKQISIGRVGDD W QDP GD
Sbjct: 512  RVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGDDFWWQDPYGD 571

Query: 1401 PMGAYWLQGVHMIHCAYNSMWMGQMIQPDWDMFQSNHLCASFHAASRAICGGPVYVSDSV 1460
            P G YWLQGVHMIHC+YNS+WMGQMIQPDWDMFQS+H+CA +HAASRAICGGPVY+SD +
Sbjct: 572  PQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAICGGPVYLSDHL 631

Query: 1461 G--GHNFDLIKQLVYPDGTIPRCQYFALPTRDCLFKNPLFDNKTILKIWNLNKYGGVIGA 1520
            G   HNFDLIK+L + DGTIPRC ++ALPTRD LFKNPLFD ++ILKI+N NK+GGVIG 
Sbjct: 632  GKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFNFNKFGGVIGT 691

Query: 1521 FNCQGAGWDPKEQRIRGRPECYKPTSTTVHIKDVEWDQKPEAAPMGNFV----EYVVYLN 1580
            FNCQGAGW P+E R +G  ECY   S TVH+ D+EWDQ PEAA  G+ V    +Y+VY  
Sbjct: 692  FNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAA--GSQVTYTGDYLVYKQ 751

Query: 1581 QAGQIVHMTSKSEPLELTLQPSTFELFNFIPIKKL-SSNIKFAPIGLTNMFNSSGTIQHL 1640
            Q+ +I+ M SKSE +++TL+PS F+L +F+P+ +L SS ++FAP+GL NMFN  GT+Q +
Sbjct: 752  QSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFAPLGLINMFNCVGTVQDM 811

Query: 1641 KY-NEKGVELKVKGVGNFLAYSSGSPKKCLSNGMDVKFEWNSD-GKLSFELPWIEEVGGV 1696
            K   +  + + VKG G F+AYSS +P KC  N  + +F+W  + GKLSF +PW+EE GG+
Sbjct: 812  KVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEETGKLSFFVPWVEESGGI 871

BLAST of Cp4.1LG12g06210 vs. Swiss-Prot
Match: RFS5_ARATH (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana GN=RFS5 PE=1 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 6.5e-150
Identity = 257/487 (52.77%), Postives = 340/487 (69.82%), Query Frame = 1

Query: 358 QKLKAELNEIFGNQEEETSSSKDDDDDSGLKAFTKDLRTKFKGLDDVFVWHALAGAWGGV 417
           +++   L +   N + +   S  D +D G+KAF +DL+ +F  +D ++VWHAL G WGG+
Sbjct: 286 EQMPCRLLKFEENHKFKDYVSPKDQNDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGL 345

Query: 418 RPGSTHLN-SKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKV 477
           RP +  L  S II  +LSPGL  TMEDLAV KIIE  IG   PD A +F++ +HS+L   
Sbjct: 346 RPEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNA 405

Query: 478 GITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYL 537
           GI GVKVDVIH LE + ++YGGRVDLAKAY+K LT+S+ K+F G G+ +SM+ CNDF +L
Sbjct: 406 GIDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFL 465

Query: 538 GTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHL 597
           GT+  S+GRVGDDFW  DP GDP G +WLQG HM+HCAYNS+WMG  IQPDWDMFQS H 
Sbjct: 466 GTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHP 525

Query: 598 CAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKNPLF 657
           CA+FHA SRAI GGPIY+SD VG HDFDL+K+LV P+G+I RC+Y+ALPTRD LF++PL 
Sbjct: 526 CAEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLH 585

Query: 658 DNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEWDQKP 717
           D KT+LKIWNLNKY GVIGAFNCQG GW  + +R +   EC   ++ T    +VEW+   
Sbjct: 586 DGKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGS 645

Query: 718 ETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKL-GSNIKFAP 777
               + N  E+ ++L+Q+ ++L  +  ++ LELTLEP  FEL    P+  + G++++FAP
Sbjct: 646 SPISIANVEEFALFLSQSKKLL-LSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAP 705

Query: 778 IGLTNMFNSSGTIQHLKYNEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEWSSDGK 837
           IGL NM N+SG I+ L YN++ VE+ V G G F  Y+S  P  CL +G  VEF +  D  
Sbjct: 706 IGLVNMLNTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGY-EDSM 765

Query: 838 LSFDLPW 843
           +   +PW
Sbjct: 766 VMVQVPW 770

BLAST of Cp4.1LG12g06210 vs. Swiss-Prot
Match: RFS_PEA (Galactinol--sucrose galactosyltransferase OS=Pisum sativum GN=RFS PE=1 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 4.2e-141
Identity = 240/455 (52.75%), Postives = 313/455 (68.79%), Query Frame = 1

Query: 380 DDDDDSGLKAFTKDLRTKFKGLDDVFVWHALAGAWGGVRPGSTHL-NSKIIPCKLSPGLD 439
           D+    GL  F +DL+ +F+ ++ V+VWHAL G WGGVRP    +  +K++  KLSPG+ 
Sbjct: 321 DNGGKKGLVGFVRDLKEEFRSVESVYVWHALCGYWGGVRPKVCGMPEAKVVVPKLSPGVK 380

Query: 440 GTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVIHTLEYVSEEYGG 499
            TMEDLAV KI+E  +GLV P+ A + FD +HS+L   GI GVKVDVIH LE +SEEYGG
Sbjct: 381 MTMEDLAVDKIVENGVGLVPPNLAQEMFDGIHSHLESAGIDGVKVDVIHLLELLSEEYGG 440

Query: 500 RVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYLGTKQNSIGRVGDDFWFQDPLGD 559
           RV+LAKAYYK LT+S+ K+FKG G+ +SM+ CNDFF LGT+  S+GRVGDDFW  DP GD
Sbjct: 441 RVELAKAYYKALTSSVNKHFKGNGVIASMEHCNDFFLLGTEAISLGRVGDDFWCCDPSGD 500

Query: 560 PMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPIYVSDSV 619
           P G YWLQG HM+HCAYNS+WMG  I PDWDMFQS H CA+FHA SRAI GGP+YVSD V
Sbjct: 501 PNGTYWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAEFHAASRAISGGPVYVSDCV 560

Query: 620 GCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKNPLFDNKTVLKIWNLNKYGGVIGAFN 679
           G H+F L+K  V PDG+I RCQ++ALPTRDCLF++PL + KT+LKIWNLNKY GV+G FN
Sbjct: 561 GNHNFKLLKSFVLPDGSILRCQHYALPTRDCLFEDPLHNGKTMLKIWNLNKYAGVLGLFN 620

Query: 680 CQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEWDQKPETAPMGNFVEYVVYLNQADQIL 739
           CQG GW P+ +R K   E    ++      ++EW        +     + VY  + ++ L
Sbjct: 621 CQGGGWCPETRRNKSASEFSHAVTCYASPEDIEWCNGKTPMDIKGVDVFAVYFFK-EKKL 680

Query: 740 HTTPKSEPLELTLEPSTFELFNFIPLKKLGSN-IKFAPIGLTNMFNSSGTIQHLKYNEKG 799
                S+ LE++LEP +FEL    PLK      I+FAPIGL NM NS G +Q L++++  
Sbjct: 681 SLMKCSDRLEVSLEPFSFELMTVSPLKVFSKRLIQFAPIGLVNMLNSGGAVQSLEFDDSA 740

Query: 800 --VELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEW 831
             V++ V+G G    ++S  P  C  +G+ VEF++
Sbjct: 741 SLVKIGVRGCGELSVFASEKPVCCKIDGVSVEFDY 774

BLAST of Cp4.1LG12g06210 vs. Swiss-Prot
Match: RFS_ORYSJ (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica GN=RFS PE=1 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.6e-140
Identity = 238/465 (51.18%), Postives = 313/465 (67.31%), Query Frame = 1

Query: 386 GLKAFTKDLRTKFKGLDDVFVWHALAGAWGGVRPGSTHLN-SKIIPCKLSPGLDGTMEDL 445
           G+  F ++++  F  ++ V+VWHAL G WGG+RPG+  L  +K++  +LSPGL  TMEDL
Sbjct: 312 GMGGFVREMKAAFPTVEQVYVWHALCGYWGGLRPGAPGLPPAKVVAPRLSPGLQRTMEDL 371

Query: 446 AVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVIHTLEYVSEEYGGRVDLAK 505
           AV KI+   +GLV P +A + ++ +HS+L   GI GVKVDVIH LE V EEYGGRV+LAK
Sbjct: 372 AVDKIVNNGVGLVDPRRARELYEGLHSHLQASGIDGVKVDVIHLLEMVCEEYGGRVELAK 431

Query: 506 AYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYLGTKQNSIGRVGDDFWFQDPLGDPMGVYW 565
           AY+ GLT S+ ++F G G+ +SM+ CNDF  LGT+  ++GRVGDDFW  DP GDP G +W
Sbjct: 432 AYFAGLTESVRRHFNGNGVIASMEHCNDFMLLGTEAVALGRVGDDFWCTDPSGDPDGTFW 491

Query: 566 LQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPIYVSDSVGCHDFD 625
           LQG HM+HCAYNS+WMG  I PDWDMFQS H CA FHA SRA+ GGP+YVSD+VGCHDFD
Sbjct: 492 LQGCHMVHCAYNSLWMGAFIHPDWDMFQSTHPCAAFHAASRAVSGGPVYVSDAVGCHDFD 551

Query: 626 LMKQLVYPDGTIPRCQYFALPTRDCLFKNPLFDNKTVLKIWNLNKYGGVIGAFNCQGAGW 685
           L+++L  PDGTI RC+ +ALPTRDCLF +PL D KT+LKIWN+NK+ GV+GAFNCQG GW
Sbjct: 552 LLRRLALPDGTILRCERYALPTRDCLFADPLHDGKTMLKIWNVNKFSGVLGAFNCQGGGW 611

Query: 686 DPKEQRIKGRPECYKPMSTTIHVNNVEWDQKPETAPMGNFVEYVVYLNQADQILHTTPKS 745
             + +R         P++      +VEW         G    + VY  +A + L    + 
Sbjct: 612 SREARRNMCAAGFSVPVTARASPADVEWSHGG-----GGGDRFAVYFVEARK-LQLLRRD 671

Query: 746 EPLELTLEPSTFELFNFIPLKKLGS---NIKFAPIGLTNMFNSSGTIQHL----KYNEKG 805
           E +ELTLEP T+EL    P++ + S    I FAPIGL NM N+ G +Q      K  +  
Sbjct: 672 ESVELTLEPFTYELLVVAPVRAIVSPELGIGFAPIGLANMLNAGGAVQGFEAARKDGDVA 731

Query: 806 VELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEWSSDGKLSFDLPW 843
            E+ VKG G  +AYSS  P+ C  NG + EF++  DG ++ D+PW
Sbjct: 732 AEVAVKGAGEMVAYSSARPRLCKVNGQDAEFKY-EDGIVTVDVPW 769

BLAST of Cp4.1LG12g06210 vs. TrEMBL
Match: B8LG99_CUCSA (Stachyose synthase OS=Cucumis sativus GN=Csa_7G407800 PE=2 SV=1)

HSP 1 Score: 1609.0 bits (4165), Expect = 0.0e+00
Identity = 760/859 (88.47%), Postives = 811/859 (94.41%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSSDA 60
           MAPPND A LN+SVLKSD LENLID SDGKI V GVP+LS+VPTNVFFSPFSSI QSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVH+LS+KGGFLGF Q+ PSDRL NSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180
           SDLQMETQWV+LN+PEI SYV+IIPIIEGSFRSA+HPGTDGQVLICAESGSTHVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPY+LMKEAYAA RVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEECER 300
           IWNGV+DFVEGGISPRFLIIDDGWQSIN DGEDPTRDAKNLVLGGTQMT+RLYRF+ECE+
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAK+IEI+  E++RDKAI SGVT+VS+FETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KAELNEIFGNQEEETSSSKDDD--------DDSGLKAFTKDLRTKFKGLDDVFVWHALAG 420
           K EL+ IFG +EEE SS+ +          D+SG+KAFT+DLRTKFKGLDD+FVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THLNSKI+PCKLSPGLDGTM DLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDV+HTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGP+YVSDSVG H+FDL+KQLVYPDGTIPRCQ+FALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEW 720
           NPLFDNKTVLKIWNLNKYGGVIG FNCQGAGWDPKEQRIKG PECYKPMSTT+HVN++EW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIK 780
           DQKPE APMGNFVEY+VYLNQA+QILHTTPKSEPL+ T++PSTFELFNFIPL+KLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEWSS 840
           FAPIGLTNMFN SGTIQHLKYNE GVELKVKG GNFLAYSSGSPKKC+SNG+EVEFEW S
Sbjct: 781 FAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIEVEFEWKS 840

Query: 841 DGKLSFDLPWIEEVATLNS 852
           DGKLSFDL WIEE   +++
Sbjct: 841 DGKLSFDLHWIEEAGGVSN 859

BLAST of Cp4.1LG12g06210 vs. TrEMBL
Match: A8CM21_CUCSA (Stachyose synthetase variant 1 OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 1608.6 bits (4164), Expect = 0.0e+00
Identity = 760/853 (89.10%), Postives = 808/853 (94.72%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSSDA 60
           MAPPND A LN+SVLKSD LENLID SDGKI V GVP+LS+VPTNVFFSPFSSI QSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVH+LS+KGGFLGF Q+ PSDRL NSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180
           SDLQMETQWV+LN+PEI SYV+IIPIIEGSFRSA+HPGTDGQVLICAESGSTHVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPY+LMKEAYAA RVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEECER 300
           IWNGV+DFVEGGISPRFLIIDDGWQSIN DGEDPTRDAKNLVLGGTQMT+RLYRF+ECE+
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAK+IEI+  E++RDKAI SGVT+VS+FETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KAELNEIFGNQEEETSSSKDDD--------DDSGLKAFTKDLRTKFKGLDDVFVWHALAG 420
           K EL+ IFG +EEE SS+ +          D+SG+KAFT+DLRTKFKGLDD+FVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THLNSKI+PCKLSPGLDGTM DLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDV+HTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGP+YVSDSVG H+FDL+KQLVYPDGTIPRCQ+FALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEW 720
           NPLFDNKTVLKIWNLNKYGGVIG FNCQGAGWDPKEQRIKG PECYKPMSTT+HVN++EW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIK 780
           DQKPE APMGNFVEY+VYLNQA+QILHTTPKSEPL+ T++PSTFELFNFIPL+KLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEWSS 840
           FAPIGLTNMFN SGTIQHLKYNE GVELKVKG GNFLAYSSGSPKKC+SNG+EVEFEW S
Sbjct: 781 FAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIEVEFEWKS 840

Query: 841 DGKLSFDLPWIEE 846
           DGKLSFDL WIEE
Sbjct: 841 DGKLSFDLHWIEE 853

BLAST of Cp4.1LG12g06210 vs. TrEMBL
Match: M5XML9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001276mg PE=4 SV=1)

HSP 1 Score: 1400.6 bits (3624), Expect = 0.0e+00
Identity = 661/865 (76.42%), Postives = 755/865 (87.28%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSSDA 60
           MAPPND  +   S+ +S+SLE   DLS+GK  V GVPLLS+VP+NV F+ F S  QSSDA
Sbjct: 1   MAPPNDPVSPILSLCRSNSLEQYFDLSNGKFSVKGVPLLSEVPSNVTFNHFHSTSQSSDA 60

Query: 61  PLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           P PL QRV  LSHKGGFLGF++  PSDRLMNSLG+F  R+F+S+FRFKTWWSTMWVGNSG
Sbjct: 61  PFPLFQRVRALSHKGGFLGFNKEEPSDRLMNSLGRFSNRDFLSIFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180
           S LQMETQWV+L+VPEI SYVII+PIIEGSFRSALHPGTD  V+ICAESGST VKAS+FD
Sbjct: 121 SSLQMETQWVLLDVPEIKSYVIILPIIEGSFRSALHPGTDDHVMICAESGSTQVKASNFD 180

Query: 181 AIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVH S+NPY LMKEAY+A RVHLNTFRLLEEK + +LVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHASENPYNLMKEAYSALRVHLNTFRLLEEKTIPNLVDKFGWCTWDAFYLTVEPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEECER 300
           +W+GVN+FVEGG+SPRFLIIDDGWQSIN DGED   DAKNLVLGGTQMT+RL+RFEEC++
Sbjct: 241 VWHGVNEFVEGGVSPRFLIIDDGWQSINLDGEDLHEDAKNLVLGGTQMTARLHRFEECKK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQKL 360
           FR Y+GGS+LGP+APSFDPKKPKLLIAK+IEI+  E++RDKAI SGVTD+SEFETKIQKL
Sbjct: 301 FRNYRGGSMLGPDAPSFDPKKPKLLIAKAIEIEHAEKNRDKAIRSGVTDLSEFETKIQKL 360

Query: 361 KAELNEIFGNQEEETSSSKDD-------DDDSGLKAFTKDLRTKFKGLDDVFVWHALAGA 420
           K EL EI G  EEE+S+S +         D+ G+KAFT DLRTKFKGLDD++VWHAL GA
Sbjct: 361 KQELEEIIG--EEESSASNEGCGSCSCGADNYGMKAFTNDLRTKFKGLDDIYVWHALCGA 420

Query: 421 WGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYL 480
           WGGVRPG+THL++K+IPCK+SPGLDGTM DLAVVKI+EG IGLV+PDQAD+ FD+MHSYL
Sbjct: 421 WGGVRPGATHLSAKVIPCKVSPGLDGTMTDLAVVKIVEGGIGLVNPDQADNLFDAMHSYL 480

Query: 481 SKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDF 540
           SKVGITGVKVDVIHTLEYVSEEYGGRV+LAKAYYKGLT+SL KNF GTGL +SM QCNDF
Sbjct: 481 SKVGITGVKVDVIHTLEYVSEEYGGRVELAKAYYKGLTHSLQKNFNGTGLIASMHQCNDF 540

Query: 541 FYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQS 600
           F+LGTKQ SIGRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQII PDWDMFQS
Sbjct: 541 FFLGTKQISIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIHPDWDMFQS 600

Query: 601 DHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKN 660
           DHLCAKFHAGSRAICGGP+YVSDSV  HDFDL+K+LVYPDGTIP+CQ+FALPTRDCLFKN
Sbjct: 601 DHLCAKFHAGSRAICGGPVYVSDSVAGHDFDLIKKLVYPDGTIPKCQHFALPTRDCLFKN 660

Query: 661 PLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEWD 720
           PLFDNKTVLKIWN NKYGGVIGAFNCQGAGWDPKEQRIKG  +CYKP+S ++HV+ +EWD
Sbjct: 661 PLFDNKTVLKIWNFNKYGGVIGAFNCQGAGWDPKEQRIKGYSDCYKPISCSLHVSELEWD 720

Query: 721 QKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIKF 780
           QK E A +    EYVVYLNQA+++   TPKS+ +++T++PSTFELF+F+P+KK+GS+IKF
Sbjct: 721 QKIEAANLCKAEEYVVYLNQAEELRLVTPKSDAIQITIQPSTFELFSFVPIKKIGSSIKF 780

Query: 781 APIGLTNMFNSSGTIQHLKYNEKGVE----LKVKGVGNFLAYSSGSPKKCLSNGMEVEFE 840
           APIGLTNMFNS GT+Q L+Y    VE    +KVKG GNFLAYSS SPKKC  NG EV FE
Sbjct: 781 APIGLTNMFNSGGTVQELEYKTTAVEFSAQMKVKGGGNFLAYSSESPKKCCLNGTEVAFE 840

Query: 841 WSSDGKLSFDLPWIEEVATLNSSVL 855
           WS+DGKL+ +LPW+EE A  NS V+
Sbjct: 841 WSTDGKLTLNLPWVEEAAG-NSDVV 862

BLAST of Cp4.1LG12g06210 vs. TrEMBL
Match: B9I9G9_POPTR (Stachyose synthase family protein OS=Populus trichocarpa GN=POPTR_0014s11370g PE=4 SV=2)

HSP 1 Score: 1371.7 bits (3549), Expect = 0.0e+00
Identity = 641/855 (74.97%), Postives = 740/855 (86.55%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLK-SDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSP-FSSICQSS 60
           MAPPND  +   S+ K  DS +   DLS+GK+ V G PLLS+VP+NV F+P FSSIC+  
Sbjct: 1   MAPPNDPTSPPLSIRKHDDSSDKYFDLSNGKLSVKGFPLLSEVPSNVTFAPLFSSICKPP 60

Query: 61  DAPLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGN 120
           DAPL LLQRV  LSHKGGFLGFH+  PSDRL+NSLGKF GREF+S+FRFKTWWSTMWVGN
Sbjct: 61  DAPLALLQRVQALSHKGGFLGFHKEAPSDRLINSLGKFTGREFLSIFRFKTWWSTMWVGN 120

Query: 121 SGSDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASS 180
           SGSDLQMETQWV+LNVPEI SYVIIIP+I+GSFRSALHPGTDG V+ICAESGST V ASS
Sbjct: 121 SGSDLQMETQWVLLNVPEIRSYVIIIPVIDGSFRSALHPGTDGHVMICAESGSTKVTASS 180

Query: 181 FDAIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDP 240
           FDAIAYVHVS+NPY +M EAY+A RVHLNTF+LLEEK    L+DKFGWCTWDAFYLTV+P
Sbjct: 181 FDAIAYVHVSENPYHIMNEAYSALRVHLNTFKLLEEKAAPSLIDKFGWCTWDAFYLTVEP 240

Query: 241 VGIWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEEC 300
            G+W+GVNDFVEGG+SPRFLIIDDGWQSIN DGE+P  DAKNLVLGGTQMT+RL+R +EC
Sbjct: 241 AGVWHGVNDFVEGGVSPRFLIIDDGWQSINFDGENPNEDAKNLVLGGTQMTARLHRLDEC 300

Query: 301 ERFRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQ 360
           E+FR+YKGGSLLGP  PSFDPKKPK+LI+K+IE++  E+DRDKAI+SGVTD+S FE+KIQ
Sbjct: 301 EKFREYKGGSLLGPRPPSFDPKKPKMLISKAIELEHAEKDRDKAIQSGVTDLSAFESKIQ 360

Query: 361 KLKAELNEIFGNQEEETSSSKDDD-----DDSGLKAFTKDLRTKFKGLDDVFVWHALAGA 420
           KLK EL+ +F   E+  S+          D  G+KAFT+DLRTKFKGLDD++VWHAL GA
Sbjct: 361 KLKQELDVMFCGDEKSVSTGSSGSCSCKADSYGMKAFTRDLRTKFKGLDDIYVWHALCGA 420

Query: 421 WGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYL 480
           WGGVRPG+THLNSKIIPCKLS GLDGTM DLAVVKIIEG IGLV PDQA DF+DSMHSYL
Sbjct: 421 WGGVRPGATHLNSKIIPCKLSAGLDGTMNDLAVVKIIEGGIGLVQPDQAGDFYDSMHSYL 480

Query: 481 SKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDF 540
           + VGITGVKVDVIHTLEYVSEEYGGRV+LAK+YY+GL++SL +NFKG+GL SSM+QCNDF
Sbjct: 481 ASVGITGVKVDVIHTLEYVSEEYGGRVELAKSYYRGLSDSLAENFKGSGLISSMEQCNDF 540

Query: 541 FYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQS 600
           F+LGTKQ S+GRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQS
Sbjct: 541 FFLGTKQISMGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQS 600

Query: 601 DHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKN 660
           DHLCAKFHAGSRAICGGP+YVSDSVG HDF+L+K+LVYPDGTIPRCQ+FALPTRDCLF+N
Sbjct: 601 DHLCAKFHAGSRAICGGPVYVSDSVGGHDFELLKKLVYPDGTIPRCQHFALPTRDCLFRN 660

Query: 661 PLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEWD 720
           PLFD KT+LKIWN NK+GGVIGAFNCQGAGWDPKE+RIKG  ECYK MS ++HV ++EWD
Sbjct: 661 PLFDKKTILKIWNFNKHGGVIGAFNCQGAGWDPKERRIKGYSECYKLMSGSVHVTDIEWD 720

Query: 721 QKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIKF 780
           QK E A MG   EY+++LNQA+ +L  +P+SE +++T+EPS+FE+F+F+P+KKLG++IKF
Sbjct: 721 QKKEAAQMGEAEEYIIHLNQAEDLLLVSPESEAMQITIEPSSFEIFSFVPIKKLGTSIKF 780

Query: 781 APIGLTNMFNSSGTIQHLKY----NEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFE 840
           APIGLTNMFNS GTIQ L Y     E  V++ VKG GNFL+YS+ SPKKC  NG EV FE
Sbjct: 781 APIGLTNMFNSGGTIQELGYFDSEAETCVKIDVKGGGNFLSYSNASPKKCFLNGAEVAFE 840

Query: 841 WSSDGKLSFDLPWIE 845
           W  +GKLS +LPW E
Sbjct: 841 WLDNGKLSLNLPWTE 855

BLAST of Cp4.1LG12g06210 vs. TrEMBL
Match: A0A067JWF2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14052 PE=4 SV=1)

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 647/862 (75.06%), Postives = 740/862 (85.85%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSS-- 60
           MAPPND A     VLKS+SL+   DLS+GK  V G PLLSDVP+NV FSPFSSIC SS  
Sbjct: 1   MAPPNDPANSLPKVLKSESLDKNFDLSNGKFSVAGFPLLSDVPSNVVFSPFSSICNSSES 60

Query: 61  DAPLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGN 120
           DAPLPLLQRV +LS++GGFLGFH+  PSDRLMNSLGKF  R+F+SVFRFKTWWSTMWVG+
Sbjct: 61  DAPLPLLQRVLSLSNRGGFLGFHKDSPSDRLMNSLGKFSHRDFLSVFRFKTWWSTMWVGD 120

Query: 121 SGSDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASS 180
           SGSDLQMETQWV+LNVPEI SYVIIIP IEG+FRSALHPG DG V+ICAESGST VKASS
Sbjct: 121 SGSDLQMETQWVLLNVPEIKSYVIIIPTIEGNFRSALHPGNDGHVMICAESGSTRVKASS 180

Query: 181 FDAIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDP 240
           F+AIAYVHVS+NPY +MKEAY+A RVHLNTFRLLEEK V  L+DKFGWCTWDAFYLTV+P
Sbjct: 181 FNAIAYVHVSENPYNIMKEAYSALRVHLNTFRLLEEKSVPSLIDKFGWCTWDAFYLTVEP 240

Query: 241 VGIWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEEC 300
            GIW GVNDFVEGG+SPRF+IIDDGWQSIN D E P +D KNLVLGGTQMT+RL+R +E 
Sbjct: 241 SGIWYGVNDFVEGGVSPRFVIIDDGWQSINYDSEKPNQDTKNLVLGGTQMTARLHRLDES 300

Query: 301 ERFRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQ 360
           E+FRKYK GS+LGPN P FDPKKPK+LI+K+ E++  E++ DKA +SGVTD+S FE+KIQ
Sbjct: 301 EKFRKYKEGSMLGPNPPKFDPKKPKMLISKATELEHAEKELDKAKQSGVTDLSIFESKIQ 360

Query: 361 KLKAELNEIFGNQE-----EETSSSKDDDDDSGLKAFTKDLRTKFKGLDDVFVWHALAGA 420
           KLK EL+ IFG +E     E   S     D+ G+KA T+DLRTKFKGLDD++VWHA+ GA
Sbjct: 361 KLKKELDAIFGEEEKFYSSEGCGSCSCKRDNYGMKALTRDLRTKFKGLDDIYVWHAIFGA 420

Query: 421 WGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYL 480
           WGGVRPG+THLNSKI+PCKLSPGLDGTMEDLAVVKI+EG IGLV P++A DF+DSMHSYL
Sbjct: 421 WGGVRPGTTHLNSKIVPCKLSPGLDGTMEDLAVVKIVEGGIGLVDPNRAGDFYDSMHSYL 480

Query: 481 SKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDF 540
           S VGITGVKVDVIH+LEYVSE+YGGRV+LAK YYKGL++S+ KNFKGTGL SSMQQCNDF
Sbjct: 481 SNVGITGVKVDVIHSLEYVSEDYGGRVELAKTYYKGLSDSVSKNFKGTGLISSMQQCNDF 540

Query: 541 FYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQS 600
           F+LGTKQ S+GRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQS
Sbjct: 541 FFLGTKQISMGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQS 600

Query: 601 DHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKN 660
           DHLCAKFHAGSRAICGGPIYVSDSV  HDFDL+K+LVYPDGTIP+CQ+FALPTRDCLFKN
Sbjct: 601 DHLCAKFHAGSRAICGGPIYVSDSVDGHDFDLLKKLVYPDGTIPKCQHFALPTRDCLFKN 660

Query: 661 PLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEWD 720
           PLFD KT+LKIWN NKYGGVIGAFNCQGAGWDPKEQRIKG  +CYKPMS ++HV ++EWD
Sbjct: 661 PLFDKKTILKIWNFNKYGGVIGAFNCQGAGWDPKEQRIKGHSDCYKPMSGSVHVTDIEWD 720

Query: 721 QKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIKF 780
           QK E A MG   EY+VYL QA ++L  TP S+ +++TL+PSTFELF+F+P+KKLG  IKF
Sbjct: 721 QKLEAAQMGKAEEYIVYLGQAQELLFMTPNSDAIQVTLKPSTFELFSFVPIKKLGPTIKF 780

Query: 781 APIGLTNMFNSSGTIQHLKY----NEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFE 840
           APIGLTNMFN  GTIQ L+Y    +E GV+++VKG G F+AYS+ SPKKC  NG EV F+
Sbjct: 781 APIGLTNMFNMGGTIQELEYFVSESEIGVKIEVKGEGKFMAYSNVSPKKCFLNGGEVGFD 840

Query: 841 WSSDGKLSFDLPWIEEVATLNS 852
           W +DGKL  DLPW EE   +++
Sbjct: 841 WLADGKLCLDLPWNEEAGGVSN 862

BLAST of Cp4.1LG12g06210 vs. TAIR10
Match: AT4G01970.1 (AT4G01970.1 stachyose synthase)

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 515/847 (60.80%), Postives = 654/847 (77.21%), Query Frame = 1

Query: 861  NLIDVSDGKLNVK-GVPLLSDVPTNVFFSPFSSICQSSDAPLPLLQRVHTLSHKGGFLGF 920
            N  ++S+G L  K   P+L DVP NV F+PFSS   S+DAPLP+L RV   +HKGGFLGF
Sbjct: 32   NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 921  HQSNPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVILNVPEINSY 980
             + +PSDRLTNSLG+F+ REF+S+FRFK WWST W+G SGSDLQ ETQWV+L +PEI+SY
Sbjct: 92   TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEIDSY 151

Query: 981  VVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFDAIAYVHVSDNPYNLMKEAYA 1040
            V IIP IEG+FR++L PG  G VLICAESGST VK SSF +IAY+H+ DNPYNLMKEA++
Sbjct: 152  VAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFS 211

Query: 1041 AIRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPAGIWSGLNEFVEGGISPRFLII 1100
            A+RVH+NTF+LLEEK +  +VDKFGWCTWDA YLTVDPA IW+G+ EF +GG+ P+F+II
Sbjct: 212  ALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVII 271

Query: 1101 DDGWQSINMDGDDPTRDTKNLVWGGTQMTSRLYSFEECEKFRKYKGGCLLEPNAPSFDPM 1160
            DDGWQSIN DGD+  +D +NLV GG QMT+RL SF+EC+KFR YKGG  +  +A  F+P+
Sbjct: 272  DDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPL 331

Query: 1161 KPKLLMNKAIE-IDEVGKNRDKAIRSGITDVSQFETKIQKLKEELNEIFERYEEQSSATS 1220
            KPK+L+ KA E I  +   R     SG  D+++ + KI+ L EELN +F+  E++ S  S
Sbjct: 332  KPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLGS 391

Query: 1221 KDGDEFGLKAFTRDLRTRFKGLDDVFVWHALAGAWGGVRPGS-THLNSKLTPCKLSPGLD 1280
             D    G+ AFT+DLR RFK LDD++VWHAL GAW GVRP +   L +K+ P +LSP L 
Sbjct: 392  DDVSGSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAPFELSPSLG 451

Query: 1281 GSMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGVTGVKVDVMHTLEYVSEEYGG 1340
             +M DLAV K++E  IGLVHP +A +F+DSMHSYL+ VGVTG K+DV  TLE ++EE+GG
Sbjct: 452  ATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQTLESLAEEHGG 511

Query: 1341 RVDLAKAYYNGLSNSLLKNFKGTGLFSSMQQCNDFFYLGTKQISIGRVGDDVWVQDPQGD 1400
            RV+LAKAYY+GL+ S++KNF GT + +SMQQCN+FF+L TKQISIGRVGDD W QDP GD
Sbjct: 512  RVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGDDFWWQDPYGD 571

Query: 1401 PMGAYWLQGVHMIHCAYNSMWMGQMIQPDWDMFQSNHLCASFHAASRAICGGPVYVSDSV 1460
            P G YWLQGVHMIHC+YNS+WMGQMIQPDWDMFQS+H+CA +HAASRAICGGPVY+SD +
Sbjct: 572  PQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAICGGPVYLSDHL 631

Query: 1461 G--GHNFDLIKQLVYPDGTIPRCQYFALPTRDCLFKNPLFDNKTILKIWNLNKYGGVIGA 1520
            G   HNFDLIK+L + DGTIPRC ++ALPTRD LFKNPLFD ++ILKI+N NK+GGVIG 
Sbjct: 632  GKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFNFNKFGGVIGT 691

Query: 1521 FNCQGAGWDPKEQRIRGRPECYKPTSTTVHIKDVEWDQKPEAAPMGNFV----EYVVYLN 1580
            FNCQGAGW P+E R +G  ECY   S TVH+ D+EWDQ PEAA  G+ V    +Y+VY  
Sbjct: 692  FNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAA--GSQVTYTGDYLVYKQ 751

Query: 1581 QAGQIVHMTSKSEPLELTLQPSTFELFNFIPIKKL-SSNIKFAPIGLTNMFNSSGTIQHL 1640
            Q+ +I+ M SKSE +++TL+PS F+L +F+P+ +L SS ++FAP+GL NMFN  GT+Q +
Sbjct: 752  QSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFAPLGLINMFNCVGTVQDM 811

Query: 1641 KY-NEKGVELKVKGVGNFLAYSSGSPKKCLSNGMDVKFEWNSD-GKLSFELPWIEEVGGV 1696
            K   +  + + VKG G F+AYSS +P KC  N  + +F+W  + GKLSF +PW+EE GG+
Sbjct: 812  KVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEETGKLSFFVPWVEESGGI 871

BLAST of Cp4.1LG12g06210 vs. TAIR10
Match: AT5G40390.1 (AT5G40390.1 Raffinose synthase family protein)

HSP 1 Score: 533.9 bits (1374), Expect = 3.7e-151
Identity = 257/487 (52.77%), Postives = 340/487 (69.82%), Query Frame = 1

Query: 358 QKLKAELNEIFGNQEEETSSSKDDDDDSGLKAFTKDLRTKFKGLDDVFVWHALAGAWGGV 417
           +++   L +   N + +   S  D +D G+KAF +DL+ +F  +D ++VWHAL G WGG+
Sbjct: 286 EQMPCRLLKFEENHKFKDYVSPKDQNDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGL 345

Query: 418 RPGSTHLN-SKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKV 477
           RP +  L  S II  +LSPGL  TMEDLAV KIIE  IG   PD A +F++ +HS+L   
Sbjct: 346 RPEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNA 405

Query: 478 GITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYL 537
           GI GVKVDVIH LE + ++YGGRVDLAKAY+K LT+S+ K+F G G+ +SM+ CNDF +L
Sbjct: 406 GIDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFL 465

Query: 538 GTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHL 597
           GT+  S+GRVGDDFW  DP GDP G +WLQG HM+HCAYNS+WMG  IQPDWDMFQS H 
Sbjct: 466 GTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHP 525

Query: 598 CAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKNPLF 657
           CA+FHA SRAI GGPIY+SD VG HDFDL+K+LV P+G+I RC+Y+ALPTRD LF++PL 
Sbjct: 526 CAEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLH 585

Query: 658 DNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEWDQKP 717
           D KT+LKIWNLNKY GVIGAFNCQG GW  + +R +   EC   ++ T    +VEW+   
Sbjct: 586 DGKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGS 645

Query: 718 ETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKL-GSNIKFAP 777
               + N  E+ ++L+Q+ ++L  +  ++ LELTLEP  FEL    P+  + G++++FAP
Sbjct: 646 SPISIANVEEFALFLSQSKKLL-LSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAP 705

Query: 778 IGLTNMFNSSGTIQHLKYNEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEWSSDGK 837
           IGL NM N+SG I+ L YN++ VE+ V G G F  Y+S  P  CL +G  VEF +  D  
Sbjct: 706 IGLVNMLNTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGY-EDSM 765

Query: 838 LSFDLPW 843
           +   +PW
Sbjct: 766 VMVQVPW 770

BLAST of Cp4.1LG12g06210 vs. TAIR10
Match: AT3G57520.1 (AT3G57520.1 seed imbibition 2)

HSP 1 Score: 338.6 bits (867), Expect = 2.3e-92
Identity = 193/542 (35.61%), Postives = 298/542 (54.98%), Query Frame = 1

Query: 1176 KNRDKAIRSGITDVSQFETKIQKLKEELNEIFERYEEQSSATSKDGDEFGLKAFTRDLRT 1235
            +N++K     + + +QF T++  +KE  N  F++ +++ +  S      GLK+   + + 
Sbjct: 244  ENKEKDENCVVQEGAQFATRLVGIKE--NAKFQKSDQKDTQVS------GLKSVVDNAKQ 303

Query: 1236 RFKGLDDVFVWHALAGAWGGVRP---GSTHLNSKLTPCKLSPGLDGSMEDLAVVKIIEGS 1295
            R   +  V+ WHALAG WGGV+P   G  H +S L     SPG+ G+  D+ +  +    
Sbjct: 304  RHN-VKQVYAWHALAGYWGGVKPAASGMEHYDSALAYPVQSPGVLGNQPDIVMDSLAVHG 363

Query: 1296 IGLVHPDQADDFFDSMHSYLSKVGVTGVKVDVMHTLEYVSEEYGGRVDLAKAYYNGLSNS 1355
            +GLV+P +  +F++ +HSYL+  G+ GVKVDV + +E +    GGRV L ++Y   L  S
Sbjct: 364  LGLVNPKKVFNFYNELHSYLASCGIDGVKVDVQNIIETLGAGLGGRVSLTRSYQQALEAS 423

Query: 1356 LLKNFKGTGLFSSMQQCNDFFYLGTKQISIGRVGDDVWVQDPQGDPMGAYWLQGVHMIHC 1415
            + +NF   G  S M    D  Y   KQ +I R  DD + +DP            +H+   
Sbjct: 424  IARNFTDNGCISCMCHNTDGLY-SAKQTAIVRASDDFYPRDPASHT--------IHIASV 483

Query: 1416 AYNSMWMGQMIQPDWDMFQSNHLCASFHAASRAICGGPVYVSDSVGGHNFDLIKQLVYPD 1475
            AYNS+++G+ +QPDWDMF S H  A +HAA+RA+ G  +YVSD  G HNFDL+++LV PD
Sbjct: 484  AYNSLFLGEFMQPDWDMFHSLHPTAEYHAAARAVGGCAIYVSDKPGNHNFDLLRKLVLPD 543

Query: 1476 GTIPRCQYFALPTRDCLFKNPLFDNKTILKIWNLNKYGGVIGAFNCQGAGW--DPKEQRI 1535
            G++ R +    PTRDCLF +P  D  ++LKIWN+NK+ G++G FNCQGAGW  + K+ +I
Sbjct: 544  GSVLRAKLPGRPTRDCLFADPARDGISLLKIWNMNKFTGIVGVFNCQGAGWCKETKKNQI 603

Query: 1536 RGRPECYKPTSTTVHIKDVEWDQKPEAAPMGNFVEYVVYLNQAGQIVHMTSKSEPLELTL 1595
                    P + T  I+  + D   + A      + +VY  ++G++V +  K   + LTL
Sbjct: 604  HDT----SPGTLTGSIRADDADLISQVAGEDWSGDSIVYAYRSGEVVRL-PKGASIPLTL 663

Query: 1596 QPSTFELFNFIPIKKLSSNIKFAPIGLTNMFNSSGTIQHLKYN---EKG----------- 1655
            +   +ELF+  P+K+++ NI FAPIGL +MFNSSG I+ +  N   +K            
Sbjct: 664  KVLEYELFHISPLKEITENISFAPIGLVDMFNSSGAIESIDINHVTDKNPEFFDGEISSA 723

Query: 1656 -------------VELKVKGVGNFLAYSSGSPKKCLSNGMDVKFEWNSD-GKLSFELPWI 1685
                         V + V+G G F AYSS  P KC     +  F ++++ G ++  LP  
Sbjct: 724  SPALSDNRSPTALVSVSVRGCGRFGAYSSQRPLKCAVESTETDFTYDAEVGLVTLNLPVT 762

BLAST of Cp4.1LG12g06210 vs. TAIR10
Match: AT5G20250.4 (AT5G20250.4 Raffinose synthase family protein)

HSP 1 Score: 332.8 bits (852), Expect = 1.2e-90
Identity = 187/506 (36.96%), Postives = 285/506 (56.32%), Query Frame = 1

Query: 338 DRDKAIESGVTDVSEFETKIQKLKAELNEIFGNQEEETSSSKDDDDDSGLKAFTKDLRTK 397
           +RD  +E+G           +K ++ +  + G +E E    KDD +  G+K   K  + K
Sbjct: 345 ERDATVEAGD----------EKKESPIFRLTGIKENEKFKKKDDPN-VGIKNIVKIAKEK 404

Query: 398 FKGLDDVFVWHALAGAWGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLV 457
             GL  V+VWHA+ G WGGVRPG  + +    P      ++        V  ++G +GLV
Sbjct: 405 H-GLKYVYVWHAITGYWGGVRPGEEYGSVMKYPNMSKGVVENDPTWKTDVMTLQG-LGLV 464

Query: 458 HPDQADDFFDSMHSYLSKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKN 517
            P +   F++ +HSYL+  G+ GVKVDV   LE +    GGRV+L + +++ L +S+ KN
Sbjct: 465 SPKKVYKFYNELHSYLADAGVDGVKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKN 524

Query: 518 FKGTGLFSSMQQCNDFFYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNS 577
           F   G  + M    D  Y  +KQ ++ R  DDF+ +DP+           +H+   AYNS
Sbjct: 525 FPDNGCIACMSHNTDALYC-SKQAAVIRASDDFYPRDPVSHT--------IHIASVAYNS 584

Query: 578 MWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIP 637
           +++G+ +QPDWDMF S H  A++HA +RAI GGP+YVSDS G H+F+L+++LV PDG+I 
Sbjct: 585 VFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSIL 644

Query: 638 RCQYFALPTRDCLFKNPLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPEC 697
           R +    PTRDCLF +P  D  ++LKIWN+NKY GV+G +NCQGA W   E++       
Sbjct: 645 RARLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTK 704

Query: 698 YKPMSTTIHVNNVEWDQKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFE 757
              ++ +I   +V    +  T P     +  VY     +++   P +  L ++L+    E
Sbjct: 705 TDSLTGSIRGRDVHSISEASTDPTTWNGDCAVYSQSRGELI-VMPYNVSLPVSLKIREHE 764

Query: 758 LFNFIPLKKLGSNIKFAPIGLTNMFNSSGTIQHLKY--NEKGVELKVKGVGNFLAYSSGS 817
           +F   P+  L   + FAPIGL NM+NS G I+ L+Y   +  V ++VKG G F +YSS  
Sbjct: 765 IFTVSPISHLVDGVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVK 824

Query: 818 PKKCLSNGMEVEFEW-SSDGKLSFDL 841
           PK+C+    E+ FE+ SS G ++F+L
Sbjct: 825 PKRCVVESNEIAFEYDSSSGLVTFEL 827

BLAST of Cp4.1LG12g06210 vs. TAIR10
Match: AT1G55740.1 (AT1G55740.1 seed imbibition 1)

HSP 1 Score: 332.4 bits (851), Expect = 1.6e-90
Identity = 186/510 (36.47%), Postives = 285/510 (55.88%), Query Frame = 1

Query: 1179 DKAIRSGITDVSQFETKIQKLKEELNEIFERYEEQSSATSKDGDEFGLKAFTRDLRTRFK 1238
            + ++     + + F  ++  +KE  N  F++  ++      D     L     D+++   
Sbjct: 247  ETSVEFNADNAANFANRLTHIKE--NHKFQKDGKEGHRV--DDPSLSLGHVITDIKSN-N 306

Query: 1239 GLDDVFVWHALAGAWGGVRPGST---HLNSKLTPCKLSPGLDGSMEDLAVVKIIEGSIGL 1298
             L  V+VWHA+ G WGGV+PG +   H  SK+     SPG+  S     +  I +  +GL
Sbjct: 307  SLKYVYVWHAITGYWGGVKPGVSGMEHYESKVAYPVSSPGVMSSENCGCLESITKNGLGL 366

Query: 1299 VHPDQADDFFDSMHSYLSKVGVTGVKVDVMHTLEYVSEEYGGRVDLAKAYYNGLSNSLLK 1358
            V+P++   F++ +HSYL+ VGV GVKVDV + LE +   +GGRV LAK Y+  L  S+ +
Sbjct: 367  VNPEKVFSFYNDLHSYLASVGVDGVKVDVQNILETLGAGHGGRVKLAKKYHQALEASISR 426

Query: 1359 NFKGTGLFSSMQQCNDFFYLGTKQISIGRVGDDVWVQDPQGDPMGAYWLQGVHMIHCAYN 1418
            NF   G+ S M    D  Y   K+ ++ R  DD W +DP            +H+   AYN
Sbjct: 427  NFPDNGIISCMSHNTDGLY-SAKKTAVIRASDDFWPRDPASHT--------IHIASVAYN 486

Query: 1419 SMWMGQMIQPDWDMFQSNHLCASFHAASRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTI 1478
            ++++G+ +QPDWDMF S H  A +HAA+RA+ G  +YVSD  G H+F+L+++LV  DG+I
Sbjct: 487  TLFLGEFMQPDWDMFHSLHPMAEYHAAARAVGGCAIYVSDKPGQHDFNLLRKLVLRDGSI 546

Query: 1479 PRCQYFALPTRDCLFKNPLFDNKTILKIWNLNKYGGVIGAFNCQGAGWDPKEQRIRGRPE 1538
             R +    PT DC F +P+ DNK++LKIWNLN++ GVIG FNCQGAGW   E+R     +
Sbjct: 547  LRAKLPGRPTSDCFFSDPVRDNKSLLKIWNLNEFTGVIGVFNCQGAGWCKNEKRYLIHDQ 606

Query: 1539 CYKPTSTTVHIKDVEWDQKPEAAPMGNFVEYVVYLNQAGQIVHMTSKSEPLELTLQPSTF 1598
                 S  V   DV +  K  A       + +VY +  G++V++  K   L +TL P  +
Sbjct: 607  EPGTISGCVRTNDVHYLHKVAAFEWTG--DSIVYSHLRGELVYL-PKDTSLPVTLMPREY 666

Query: 1599 ELFNFIPIKKLSSNIKFAPIGLTNMFNSSGTIQHLKYNEKG----VELKVKGVGNFLAYS 1658
            E+F  +P+K+ S   KFAP+GL  MFNS G I  L+Y+++G    V +K++G G    YS
Sbjct: 667  EVFTVVPVKEFSDGSKFAPVGLMEMFNSGGAIVSLRYDDEGTKFVVRMKLRGSGLVGVYS 726

Query: 1659 S-GSPKKCLSNGMDVKFEWNSD-GKLSFEL 1680
            S   P+    +  DV++ +  + G ++F L
Sbjct: 727  SVRRPRSVTVDSDDVEYRYEPESGLVTFTL 739

BLAST of Cp4.1LG12g06210 vs. NCBI nr
Match: gi|525507270|ref|NP_001267675.1| (steryl-sulfatase [Cucumis sativus])

HSP 1 Score: 1609.0 bits (4165), Expect = 0.0e+00
Identity = 760/859 (88.47%), Postives = 811/859 (94.41%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSSDA 60
           MAPPND A LN+SVLKSD LENLID SDGKI V GVP+LS+VPTNVFFSPFSSI QSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVH+LS+KGGFLGF Q+ PSDRL NSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180
           SDLQMETQWV+LN+PEI SYV+IIPIIEGSFRSA+HPGTDGQVLICAESGSTHVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPY+LMKEAYAA RVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEECER 300
           IWNGV+DFVEGGISPRFLIIDDGWQSIN DGEDPTRDAKNLVLGGTQMT+RLYRF+ECE+
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAK+IEI+  E++RDKAI SGVT+VS+FETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KAELNEIFGNQEEETSSSKDDD--------DDSGLKAFTKDLRTKFKGLDDVFVWHALAG 420
           K EL+ IFG +EEE SS+ +          D+SG+KAFT+DLRTKFKGLDD+FVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THLNSKI+PCKLSPGLDGTM DLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDV+HTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGP+YVSDSVG H+FDL+KQLVYPDGTIPRCQ+FALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEW 720
           NPLFDNKTVLKIWNLNKYGGVIG FNCQGAGWDPKEQRIKG PECYKPMSTT+HVN++EW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIK 780
           DQKPE APMGNFVEY+VYLNQA+QILHTTPKSEPL+ T++PSTFELFNFIPL+KLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEWSS 840
           FAPIGLTNMFN SGTIQHLKYNE GVELKVKG GNFLAYSSGSPKKC+SNG+EVEFEW S
Sbjct: 781 FAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIEVEFEWKS 840

Query: 841 DGKLSFDLPWIEEVATLNS 852
           DGKLSFDL WIEE   +++
Sbjct: 841 DGKLSFDLHWIEEAGGVSN 859

BLAST of Cp4.1LG12g06210 vs. NCBI nr
Match: gi|157326771|gb|ABV44498.1| (stachyose synthetase variant 1 [Cucumis sativus])

HSP 1 Score: 1608.6 bits (4164), Expect = 0.0e+00
Identity = 760/853 (89.10%), Postives = 808/853 (94.72%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSSDA 60
           MAPPND A LN+SVLKSD LENLID SDGKI V GVP+LS+VPTNVFFSPFSSI QSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVH+LS+KGGFLGF Q+ PSDRL NSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180
           SDLQMETQWV+LN+PEI SYV+IIPIIEGSFRSA+HPGTDGQVLICAESGSTHVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPY+LMKEAYAA RVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEECER 300
           IWNGV+DFVEGGISPRFLIIDDGWQSIN DGEDPTRDAKNLVLGGTQMT+RLYRF+ECE+
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAK+IEI+  E++RDKAI SGVT+VS+FETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KAELNEIFGNQEEETSSSKDDD--------DDSGLKAFTKDLRTKFKGLDDVFVWHALAG 420
           K EL+ IFG +EEE SS+ +          D+SG+KAFT+DLRTKFKGLDD+FVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THLNSKI+PCKLSPGLDGTM DLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDV+HTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGP+YVSDSVG H+FDL+KQLVYPDGTIPRCQ+FALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEW 720
           NPLFDNKTVLKIWNLNKYGGVIG FNCQGAGWDPKEQRIKG PECYKPMSTT+HVN++EW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIK 780
           DQKPE APMGNFVEY+VYLNQA+QILHTTPKSEPL+ T++PSTFELFNFIPL+KLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEWSS 840
           FAPIGLTNMFN SGTIQHLKYNE GVELKVKG GNFLAYSSGSPKKC+SNG+EVEFEW S
Sbjct: 781 FAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIEVEFEWKS 840

Query: 841 DGKLSFDLPWIEE 846
           DGKLSFDL WIEE
Sbjct: 841 DGKLSFDLHWIEE 853

BLAST of Cp4.1LG12g06210 vs. NCBI nr
Match: gi|659101177|ref|XP_008451468.1| (PREDICTED: stachyose synthase [Cucumis melo])

HSP 1 Score: 1608.6 bits (4164), Expect = 0.0e+00
Identity = 760/859 (88.47%), Postives = 812/859 (94.53%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSSDA 60
           MAPPND A LN+SVLKSD LENLID SDGKI V GVP+LS+VP NVFFSPFSSI QSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVH+LS+KGGFLGF Q+ PSDRL NSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180
           SDLQMETQWV+LN+PEI SYV+IIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPY+LMKEAYAA RVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEECER 300
           IWNGVNDFVEGGISPRFLIIDDGWQSIN DGEDPTRDAKNLVLGGTQMT+RLY F+ECE+
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYSFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQKL 360
           FRKYKGGSL+GPNAPSFDPKKPKLLIAK+IEI+  E++RDKAI SGVT+VS+FETKIQKL
Sbjct: 301 FRKYKGGSLMGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KAELNEIFGNQEEETSSSKDDD--------DDSGLKAFTKDLRTKFKGLDDVFVWHALAG 420
           K EL EIFGN+EEE S++ +          D+SG+KAFT+DLRTKFKGLDD+FVWHALAG
Sbjct: 361 KEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THLNSKIIPCKLSPGLDGTM DLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDV+HTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGP+YVSDSVG H+FDL+KQLVYPDGTIPRCQ+FALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEW 720
           NPLFDNKTVLKIWNLNKYGGVIG FNCQGAGWDPKEQRIKG PECYKPMSTT+HVN++EW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIK 780
           DQKPE APMGNFVEY+VYLNQA+QI+HTTPKSEPL+ T++PSTFELFNFIPL+KLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQIIHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGVGNFLAYSSGSPKKCLSNGMEVEFEWSS 840
           FAPIGLTNMFNSSGTIQHLKYNE GVELKVKG GNFLAYSSGSPKKCLSNG E++F W+S
Sbjct: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGTELKFVWNS 840

Query: 841 DGKLSFDLPWIEEVATLNS 852
           DGKLSFD+ WIEE   +++
Sbjct: 841 DGKLSFDVHWIEEAGGISN 859

BLAST of Cp4.1LG12g06210 vs. NCBI nr
Match: gi|596113381|ref|XP_007221554.1| (hypothetical protein PRUPE_ppa001276mg [Prunus persica])

HSP 1 Score: 1400.6 bits (3624), Expect = 0.0e+00
Identity = 661/865 (76.42%), Postives = 755/865 (87.28%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSSDA 60
           MAPPND  +   S+ +S+SLE   DLS+GK  V GVPLLS+VP+NV F+ F S  QSSDA
Sbjct: 1   MAPPNDPVSPILSLCRSNSLEQYFDLSNGKFSVKGVPLLSEVPSNVTFNHFHSTSQSSDA 60

Query: 61  PLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           P PL QRV  LSHKGGFLGF++  PSDRLMNSLG+F  R+F+S+FRFKTWWSTMWVGNSG
Sbjct: 61  PFPLFQRVRALSHKGGFLGFNKEEPSDRLMNSLGRFSNRDFLSIFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180
           S LQMETQWV+L+VPEI SYVII+PIIEGSFRSALHPGTD  V+ICAESGST VKAS+FD
Sbjct: 121 SSLQMETQWVLLDVPEIKSYVIILPIIEGSFRSALHPGTDDHVMICAESGSTQVKASNFD 180

Query: 181 AIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVH S+NPY LMKEAY+A RVHLNTFRLLEEK + +LVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHASENPYNLMKEAYSALRVHLNTFRLLEEKTIPNLVDKFGWCTWDAFYLTVEPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEECER 300
           +W+GVN+FVEGG+SPRFLIIDDGWQSIN DGED   DAKNLVLGGTQMT+RL+RFEEC++
Sbjct: 241 VWHGVNEFVEGGVSPRFLIIDDGWQSINLDGEDLHEDAKNLVLGGTQMTARLHRFEECKK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQKL 360
           FR Y+GGS+LGP+APSFDPKKPKLLIAK+IEI+  E++RDKAI SGVTD+SEFETKIQKL
Sbjct: 301 FRNYRGGSMLGPDAPSFDPKKPKLLIAKAIEIEHAEKNRDKAIRSGVTDLSEFETKIQKL 360

Query: 361 KAELNEIFGNQEEETSSSKDD-------DDDSGLKAFTKDLRTKFKGLDDVFVWHALAGA 420
           K EL EI G  EEE+S+S +         D+ G+KAFT DLRTKFKGLDD++VWHAL GA
Sbjct: 361 KQELEEIIG--EEESSASNEGCGSCSCGADNYGMKAFTNDLRTKFKGLDDIYVWHALCGA 420

Query: 421 WGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYL 480
           WGGVRPG+THL++K+IPCK+SPGLDGTM DLAVVKI+EG IGLV+PDQAD+ FD+MHSYL
Sbjct: 421 WGGVRPGATHLSAKVIPCKVSPGLDGTMTDLAVVKIVEGGIGLVNPDQADNLFDAMHSYL 480

Query: 481 SKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDF 540
           SKVGITGVKVDVIHTLEYVSEEYGGRV+LAKAYYKGLT+SL KNF GTGL +SM QCNDF
Sbjct: 481 SKVGITGVKVDVIHTLEYVSEEYGGRVELAKAYYKGLTHSLQKNFNGTGLIASMHQCNDF 540

Query: 541 FYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQS 600
           F+LGTKQ SIGRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQII PDWDMFQS
Sbjct: 541 FFLGTKQISIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIHPDWDMFQS 600

Query: 601 DHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKN 660
           DHLCAKFHAGSRAICGGP+YVSDSV  HDFDL+K+LVYPDGTIP+CQ+FALPTRDCLFKN
Sbjct: 601 DHLCAKFHAGSRAICGGPVYVSDSVAGHDFDLIKKLVYPDGTIPKCQHFALPTRDCLFKN 660

Query: 661 PLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEWD 720
           PLFDNKTVLKIWN NKYGGVIGAFNCQGAGWDPKEQRIKG  +CYKP+S ++HV+ +EWD
Sbjct: 661 PLFDNKTVLKIWNFNKYGGVIGAFNCQGAGWDPKEQRIKGYSDCYKPISCSLHVSELEWD 720

Query: 721 QKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIKF 780
           QK E A +    EYVVYLNQA+++   TPKS+ +++T++PSTFELF+F+P+KK+GS+IKF
Sbjct: 721 QKIEAANLCKAEEYVVYLNQAEELRLVTPKSDAIQITIQPSTFELFSFVPIKKIGSSIKF 780

Query: 781 APIGLTNMFNSSGTIQHLKYNEKGVE----LKVKGVGNFLAYSSGSPKKCLSNGMEVEFE 840
           APIGLTNMFNS GT+Q L+Y    VE    +KVKG GNFLAYSS SPKKC  NG EV FE
Sbjct: 781 APIGLTNMFNSGGTVQELEYKTTAVEFSAQMKVKGGGNFLAYSSESPKKCCLNGTEVAFE 840

Query: 841 WSSDGKLSFDLPWIEEVATLNSSVL 855
           WS+DGKL+ +LPW+EE A  NS V+
Sbjct: 841 WSTDGKLTLNLPWVEEAAG-NSDVV 862

BLAST of Cp4.1LG12g06210 vs. NCBI nr
Match: gi|645243138|ref|XP_008227841.1| (PREDICTED: stachyose synthase-like [Prunus mume])

HSP 1 Score: 1385.2 bits (3584), Expect = 0.0e+00
Identity = 653/865 (75.49%), Postives = 752/865 (86.94%), Query Frame = 1

Query: 1   MAPPNDTATLNSSVLKSDSLENLIDLSDGKIHVNGVPLLSDVPTNVFFSPFSSICQSSDA 60
           MAPP+D  +   S+ +S+ LE   DLS+GK  V GVPLLS+VP+NV F+ F S  QSSDA
Sbjct: 1   MAPPDDPVSPILSLCRSNGLEQYFDLSNGKFSVKGVPLLSEVPSNVTFNHFHSTSQSSDA 60

Query: 61  PLPLLQRVHTLSHKGGFLGFHQSHPSDRLMNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           P PL+QRV  LSHKGGFLGF++  PSDRLMNSLG+F  R+F+S+FRFKTWWSTMWVGNSG
Sbjct: 61  PFPLIQRVRALSHKGGFLGFNKEEPSDRLMNSLGRFSNRDFLSIFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVILNVPEINSYVIIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180
           S LQMETQWV+L+VPEI SYVII+PIIEGSFRSALHPGTD  V+ICAESGST V+AS+FD
Sbjct: 121 SSLQMETQWVLLDVPEIKSYVIILPIIEGSFRSALHPGTDDHVMICAESGSTQVQASNFD 180

Query: 181 AIAYVHVSDNPYKLMKEAYAATRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVH S+NPY LMKEAY+A RVHLNT RLLEEK V +LVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHASENPYNLMKEAYSALRVHLNTLRLLEEKTVPNLVDKFGWCTWDAFYLTVEPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINKDGEDPTRDAKNLVLGGTQMTSRLYRFEECER 300
           +W+GV +FVEGG+SPRFLIIDDGWQSIN DGED   DAKNLVL GTQMT+RL+RFEEC++
Sbjct: 241 VWHGVKEFVEGGVSPRFLIIDDGWQSINLDGEDLHEDAKNLVLSGTQMTARLHRFEECKK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKSIEIDRVEQDRDKAIESGVTDVSEFETKIQKL 360
           FR Y+GGS+LGP+APSFDPKKPKLLIAK+IEI+  E++RDKAI+SGVTD+SEFETKIQKL
Sbjct: 301 FRNYRGGSMLGPDAPSFDPKKPKLLIAKAIEIEHAEKNRDKAIQSGVTDLSEFETKIQKL 360

Query: 361 KAELNEIFGNQEEETSSSKDD-------DDDSGLKAFTKDLRTKFKGLDDVFVWHALAGA 420
           K EL EI G  EEE+S+S +         D+ G+KAFT DLRTKFKGLDD++VWHAL GA
Sbjct: 361 KQELKEIIG--EEESSASNEGCGSCSCGADNYGMKAFTSDLRTKFKGLDDIYVWHALCGA 420

Query: 421 WGGVRPGSTHLNSKIIPCKLSPGLDGTMEDLAVVKIIEGSIGLVHPDQADDFFDSMHSYL 480
           WGGVRPG+THL++K+IPCK+SPGLDGTM DLAVVKI+EG IGLV+PDQAD+ FD+MHSYL
Sbjct: 421 WGGVRPGATHLSAKVIPCKVSPGLDGTMTDLAVVKIVEGGIGLVNPDQADNLFDAMHSYL 480

Query: 481 SKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDF 540
           SKVGITGVKVDVIHTLEYVSEEYGGRV+LAKAYYKGLT+SL KNF GTGL +SM QCNDF
Sbjct: 481 SKVGITGVKVDVIHTLEYVSEEYGGRVELAKAYYKGLTHSLQKNFNGTGLIASMHQCNDF 540

Query: 541 FYLGTKQNSIGRVGDDFWFQDPLGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQS 600
           F+LGTKQ SIGRVGDDFWFQDP GDPMGVYWLQGVHMIHCAYNSMWMGQII PDWDMFQS
Sbjct: 541 FFLGTKQISIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIHPDWDMFQS 600

Query: 601 DHLCAKFHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKN 660
           DH CAKFHAGSRAICGGP+YVSDSV  HDF+L+K+LVYPDGTIP+CQ+FALPTRDCLFKN
Sbjct: 601 DHPCAKFHAGSRAICGGPVYVSDSVAGHDFNLIKKLVYPDGTIPKCQHFALPTRDCLFKN 660

Query: 661 PLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTIHVNNVEWD 720
           PLFDNKTVLKIWN NKYGGVIGAFNCQGAGWDPKEQRIKG  +CYKP+S ++HV+ +EWD
Sbjct: 661 PLFDNKTVLKIWNFNKYGGVIGAFNCQGAGWDPKEQRIKGYSDCYKPISCSLHVSEIEWD 720

Query: 721 QKPETAPMGNFVEYVVYLNQADQILHTTPKSEPLELTLEPSTFELFNFIPLKKLGSNIKF 780
           QK E A +    EYVVYLNQA+++   TPKS+ +++T++PSTFELF+F+P+KK+GS+IKF
Sbjct: 721 QKIEAANLCKAEEYVVYLNQAEELRLVTPKSDAIQITIQPSTFELFSFVPIKKIGSSIKF 780

Query: 781 APIGLTNMFNSSGTIQHLKYNEKGVE----LKVKGVGNFLAYSSGSPKKCLSNGMEVEFE 840
           APIGLTNMFNS G++Q L+Y    VE    +KVKG GNFLAYSS SPKKC  NG EV FE
Sbjct: 781 APIGLTNMFNSGGSVQELEYKTTAVEFSAQMKVKGGGNFLAYSSESPKKCCLNGTEVAFE 840

Query: 841 WSSDGKLSFDLPWIEEVATLNSSVL 855
           WS+DGKL+ +LPW+EE A  NS V+
Sbjct: 841 WSTDGKLTLNLPWVEEAAG-NSDVV 862