Cp4.1LG12g04220 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG12g04220 vs. Swiss-Prot
Match: INV1_DAUCA (Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota GN=INV1 PE=1 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.0e-232
Identity = 386/575 (67.13%), Postives = 464/575 (80.70%), Query Frame = 1

Query: 5   TLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHW 64
           +LLAILL  +  +L ++     V+A H+ +  LQS+   N   +KQ+HRTG+HFQP ++W
Sbjct: 20  SLLAILL-VTTTTLHING----VEAFHEIHYNLQSVGAEN---VKQVHRTGYHFQPKQNW 79

Query: 65  MNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDING 124
           +NDPNGPMY+KG+YHLFYQYNP+GAVWGNIVWAHS+S DLINW  L+PA+ PSKPFD  G
Sbjct: 80  INDPNGPMYYKGVYHLFYQYNPKGAVWGNIVWAHSVSTDLINWTPLEPAIFPSKPFDKYG 139

Query: 125 CWSGSATVLPGLKPVILYTGI---DSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG 184
           C SGSAT+LPG KPVILYTGI     +N QVQNYAIPANLSDP+L +W+KPDNNP+V   
Sbjct: 140 CRSGSATILPGNKPVILYTGIVEGPPKNVQVQNYAIPANLSDPYLRKWIKPDNNPLVVAN 199

Query: 185 TEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNT 244
              N++AFRDP+TAWL  +GHWK ++GS+  + G+AYLYRSKDF+ WTK + P+HS  NT
Sbjct: 200 NGENATAFRDPTTAWLDKSGHWKMLVGSKRNRRGIAYLYRSKDFIKWTKAKHPIHSQANT 259

Query: 245 GMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDN 304
           GMWECPDF+PVS  G  GLDTSVTG  VKHVLKVSL++TRYEYYTVG Y    D+Y+PDN
Sbjct: 260 GMWECPDFFPVSLKGLNGLDTSVTGESVKHVLKVSLDLTRYEYYTVGTYLTDKDRYIPDN 319

Query: 305 TSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTV 364
           TS DGW+GLRYDYGNFYASK+F+DP K RR+LWGWANESDS   DV+KGWAGIQ IPRT+
Sbjct: 320 TSVDGWAGLRYDYGNFYASKTFFDPSKNRRILWGWANESDSTAHDVAKGWAGIQLIPRTL 379

Query: 365 WLDPNGKQLLQWPIMELNRLR-RKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSL 424
           WLDP+GKQL+QWPI EL  LR  K+     Q + KG  VE+KGITAAQADVEV FSF SL
Sbjct: 380 WLDPSGKQLMQWPIEELETLRGSKVKFSRKQDLSKGILVEVKGITAAQADVEVTFSFKSL 439

Query: 425 DNAETFDPSWV--DAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNH 484
              E FDP W+  DA+ +CS  GST QGGVGPFGLLTLASE LEE+TPVFFR+FKA+  H
Sbjct: 440 AKREPFDPKWLEYDAEKICSLKGSTVQGGVGPFGLLTLASEKLEEYTPVFFRVFKAQNTH 499

Query: 485 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSS-KKLPLRSLIDHSVIESFGGGGKTCITS 544
            VLMCSDA  SSLK+GLY+P+FAGFVD+DL++ KK+ LRSLID+SV+ESFG  GKTCI+S
Sbjct: 500 KVLMCSDATRSSLKEGLYRPSFAGFVDVDLATDKKISLRSLIDNSVVESFGAKGKTCISS 559

Query: 545 RVYPTKAVFEDAHLHVFNNGTESITVDYLSAWSMR 573
           RVYPT AV+E+AHL+VFNNG+E+ITV+ L AWSM+
Sbjct: 560 RVYPTLAVYENAHLYVFNNGSETITVENLDAWSMK 586

BLAST of Cp4.1LG12g04220 vs. Swiss-Prot
Match: INV3_DAUCA (Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota GN=INV3 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 1.7e-227
Identity = 377/573 (65.79%), Postives = 449/573 (78.36%), Query Frame = 1

Query: 7   LAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHWMN 66
           L +L  FSF  L ++     V + H+ +P+LQS+   +  ++K +HRTG+HFQP +HW+N
Sbjct: 15  LFLLSIFSFIFLNING----VDSTHRVFPELQSI---SAVDVKLVHRTGYHFQPQKHWIN 74

Query: 67  DPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCW 126
           DPNGPM++KG YHLFYQYNP+G+VWGNIVWAHS+SKDLINW +L+PA+ PSKPFD  GCW
Sbjct: 75  DPNGPMFYKGYYHLFYQYNPKGSVWGNIVWAHSVSKDLINWIALEPAIFPSKPFDQYGCW 134

Query: 127 SGSATVLPGLKPVILYTGI---DSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTE 186
           SGSAT+LPG KPVILYTGI   D +N QVQNYA+PAN SDP L EWVKPDNNP+V   TE
Sbjct: 135 SGSATILPGNKPVILYTGIVSPDPENAQVQNYAVPANYSDPFLREWVKPDNNPLVGVHTE 194

Query: 187 VNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGM 246
            N SAFRDP+TAW    GHWK ++GS  +  G+AYLYRSKDF  W +   P+H+   TGM
Sbjct: 195 -NPSAFRDPTTAWFD-GGHWKMLVGSSRKHRGIAYLYRSKDFKKWKRSPHPIHTKAETGM 254

Query: 247 WECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTS 306
           WECPDFYPVS     GLD S  G  +KHVLKVSL  TRYEYYT+G+Y  + D YVPDNTS
Sbjct: 255 WECPDFYPVSPRSEDGLDNSKMGRGIKHVLKVSLNSTRYEYYTIGRYNRVRDFYVPDNTS 314

Query: 307 ADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWL 366
            DGW+GLRYDYGNFYASK+FYDP+KKRR+LWGWANESDS  DDV KGWAGIQ IPR +WL
Sbjct: 315 VDGWAGLRYDYGNFYASKTFYDPIKKRRILWGWANESDSQIDDVQKGWAGIQLIPRRIWL 374

Query: 367 DPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSLDNA 426
           DP+G+QL+QWPI E+  LR   + M NQK+  G +VE+ GITAAQADV+  FSF SLD A
Sbjct: 375 DPSGRQLVQWPIEEVEGLRGSELHMRNQKLDMGVHVEVTGITAAQADVDATFSFKSLDKA 434

Query: 427 ETFDPSWV--DAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHV-V 486
           E+FDP W+  DAQ VC  +GST QGG+GPFGLLTLAS++LEE+TPVFFRIFKAE   + V
Sbjct: 435 ESFDPEWINLDAQDVCDSMGSTIQGGLGPFGLLTLASKDLEEYTPVFFRIFKAEDQKLKV 494

Query: 487 LMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVY 546
           LMCSDA+ SSL +GLYKP+F GFVD+DLS KK+ LRSLID+SV+ESFG   K  I+SRVY
Sbjct: 495 LMCSDAKRSSLAEGLYKPSFRGFVDVDLSDKKISLRSLIDNSVVESFGAQRKNLISSRVY 554

Query: 547 PTKAVFEDAHLHVFNNGTESITVDYLSAWSMRS 574
           PT A++ +AHL VFNNGTE ITVD L AWSM S
Sbjct: 555 PTLAIYNNAHLFVFNNGTEPITVDNLDAWSMNS 578

BLAST of Cp4.1LG12g04220 vs. Swiss-Prot
Match: INV2_DAUCA (Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota GN=INV2 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 1.9e-226
Identity = 371/553 (67.09%), Postives = 447/553 (80.83%), Query Frame = 1

Query: 27  VQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYNP 86
           V+A H+ +P+LQS+   N   ++ +HRTG+HFQP +HW+NDPNGPMY+KG YHLFYQYNP
Sbjct: 38  VEASHQVFPELQSVSAVN---VQLVHRTGYHFQPKKHWINDPNGPMYYKGFYHLFYQYNP 97

Query: 87  EGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGID 146
           +GAVWGNIVWAHSISKDLINW +L+PA+ PSKPFD  GCWSGSATVLPG KPVI+YTGI 
Sbjct: 98  KGAVWGNIVWAHSISKDLINWVALEPAIFPSKPFDKYGCWSGSATVLPGGKPVIMYTGIV 157

Query: 147 SQ---NRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLSLNGHW 206
           +    N QVQN+A+PAN SDP+L EW+KPDNNPIV   +E NSS+FRDP+TAW     HW
Sbjct: 158 TPSPVNTQVQNFAVPANYSDPYLREWIKPDNNPIVRARSE-NSSSFRDPTTAWFD-GVHW 217

Query: 207 KTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFGRFGLDTS 266
           K ++GSR +  G+AYLYRS++FL WTK + PLHS   TGMWEC DFYPV+  G  GLDTS
Sbjct: 218 KILVGSRRKHRGIAYLYRSRNFLKWTKAKHPLHSKDRTGMWECLDFYPVAPKGMNGLDTS 277

Query: 267 VTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSF 326
           VTG  +KHVLKVSL  TRYEYYTVG+Y    D YVPDNTS DGW+GLRYDYGNFYASK+F
Sbjct: 278 VTGQDIKHVLKVSLYSTRYEYYTVGEYDRDNDIYVPDNTSVDGWAGLRYDYGNFYASKTF 337

Query: 327 YDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRR 386
           +DP K+RR+LWGWANESDS QDDV KGWAGIQ IPR +WLDPNGKQL+QWPI E+  LR 
Sbjct: 338 FDPDKQRRILWGWANESDSKQDDVQKGWAGIQLIPRKLWLDPNGKQLIQWPIEEIQLLRG 397

Query: 387 KIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSLDNAETFDPSW--VDAQAVCSQLG 446
           + V M +Q +  G+++E+KG+TAAQADV+  FSF SLD AE FDP+W  +DA  VC  +G
Sbjct: 398 QNVHMGSQVLNTGEHIEVKGVTAAQADVDATFSFKSLDRAEWFDPNWAKLDALDVCDWMG 457

Query: 447 STTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCSDA-RSSSLKDGLYKPTF 506
           ST +GG+GPFG LTLASE LEE+TPVFFR+FK +    VLMCSDA RSS+  +GLYKP F
Sbjct: 458 STVRGGLGPFGFLTLASEKLEEYTPVFFRVFKTKDKLKVLMCSDAKRSSTTAEGLYKPPF 517

Query: 507 AGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEDAHLHVFNNGTES 566
           AG+VD+DLS KK+ LRSLID+SV+ESFG  G+TCITSRVYP  A++ +AH+ VFNNGTE+
Sbjct: 518 AGYVDVDLSDKKISLRSLIDNSVVESFGAHGRTCITSRVYPKIAIYNNAHVFVFNNGTEA 577

Query: 567 ITVDYLSAWSMRS 574
           IT+D L AWSM++
Sbjct: 578 ITIDSLDAWSMKA 585

BLAST of Cp4.1LG12g04220 vs. Swiss-Prot
Match: INV4_ARATH (Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana GN=CWINV4 PE=2 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 2.8e-206
Identity = 331/557 (59.43%), Postives = 424/557 (76.12%), Query Frame = 1

Query: 27  VQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYNP 86
           + A H+ Y +LQS    +   +  LHR  FHFQP +HW+NDPNGP+Y+KG+YHLFYQYN 
Sbjct: 27  IDAFHQIYEELQS---ESVESVNHLHRPSFHFQPPKHWINDPNGPVYYKGLYHLFYQYNT 86

Query: 87  EGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGID 146
           +GAVWGNI+WAHS+SKDL+NW++L+PALSPSK FDI G WSGS T++PG  P+ILYTG++
Sbjct: 87  KGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTGVN 146

Query: 147 SQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLSLNGHWKTI 206
               Q+QNYAIP + SDP+L +W+KPD+NPI  P   +N SAFRDP+TAW S +GHW+T+
Sbjct: 147 QNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTMNGSAFRDPTTAWFSKDGHWRTV 206

Query: 207 IGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDTSVT 266
           +GS+ ++ G+AY+YRS+DF +W K + P+HS  +TGMWECPDF+PVS    R GLD    
Sbjct: 207 VGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMWECPDFFPVSLTDFRNGLDLDYV 266

Query: 267 GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYD 326
           G   KHVLKVSL++TRYEYYT+GKY    D+Y+PD  + DGW GLR+DYGNFYASK+F+D
Sbjct: 267 GPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNTPDGWEGLRFDYGNFYASKTFFD 326

Query: 327 PMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRRKI 386
             K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV LD + KQL+ WP+ E+  LR   
Sbjct: 327 YKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLLDSSKKQLVFWPVEEIESLRGNY 386

Query: 387 VLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSLDNAETFDPSWV-DAQAVCSQLGSTT 446
           V M N  +  GQ +E+KGIT AQADVEV F   SL+ AE FDPS+      +C+  GS  
Sbjct: 387 VRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEIFDPSFTWKPLELCNIKGSNV 446

Query: 447 QGGVGPFGLLTLASENLEEFTPVFFRIFKAETNH--VVLMCSDARSSSL--------KDG 506
           +GGVGPFGL+TLA+ +LEE+TPVFFR+F     H   VLMCSDAR SSL        KD 
Sbjct: 447 RGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPKVLMCSDARPSSLKQDTGLLAKDR 506

Query: 507 LYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEDAHLHVF 566
           +YKP+FAGFVD+D++  ++ LRSLIDHSV+ESFG  GKT ITSRVYP KAV E+AHL+VF
Sbjct: 507 MYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGKTVITSRVYPVKAVKENAHLYVF 566

Query: 567 NNGTESITVDYLSAWSM 572
           NNGT+++T++ L+AW+M
Sbjct: 567 NNGTQTVTIESLNAWNM 580

BLAST of Cp4.1LG12g04220 vs. Swiss-Prot
Match: INV2_ARATH (Beta-fructofuranosidase, insoluble isoenzyme CWINV2 OS=Arabidopsis thaliana GN=CWINV2 PE=2 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 1.6e-201
Identity = 341/583 (58.49%), Postives = 427/583 (73.24%), Query Frame = 1

Query: 3   FFTLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHR 62
           +  LL +L+  S      +NG   V A HK + KLQS     ++ +  LHRT +HFQP R
Sbjct: 8   YVLLLIVLINIS------NNG---VDAFHKVFKKLQSKSTSLES-VSPLHRTAYHFQPPR 67

Query: 63  HWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDI 122
           HW+NDPN PM +KG+YHLFYQYNP+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FDI
Sbjct: 68  HWINDPNAPMLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDI 127

Query: 123 NGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGT 182
           NG WSGSAT +PG  PVILYTGI     Q+QNYAIP +LSDP+L  W+KPD+NPIV P  
Sbjct: 128 NGTWSGSATHVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDN 187

Query: 183 EVNSSAFRDPSTAWLS-LNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNT 242
             N SAFRDP+TAW +  +G+W+ ++GS+ +  G+AY+Y+S+DF  W K ++P+HS   T
Sbjct: 188 GENGSAFRDPTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKT 247

Query: 243 GMWECPDFYPVSHFG-RFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPD 302
           GMWECPDF+PVS    + GLD S  G   KHVLKVSL++TRYEYYT+G Y    D+Y PD
Sbjct: 248 GMWECPDFFPVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPD 307

Query: 303 NTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRT 362
             + DGW GLR+DYGN+YASK+F+D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT
Sbjct: 308 GYTPDGWDGLRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRT 367

Query: 363 VWLDPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSL 422
           + LD +GKQL+ WPI E+  LR K V M NQK+  GQ  E++GIT AQ DV+V F+  +L
Sbjct: 368 ILLDSSGKQLVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNL 427

Query: 423 DNAETFDPSWVDAQ-AVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFK--AETN 482
           + AE FD S+      +C+  GS   GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A   
Sbjct: 428 EKAEKFDESFATKPLELCNLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNK 487

Query: 483 HVVLMCSDARSSSL--------KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGG 542
             VLMCSDA+ SSL        K+ +YKP+FAGFVD+ L   K+ LRSLIDHSV+ESFG 
Sbjct: 488 PKVLMCSDAKPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGA 547

Query: 543 GGKTCITSRVYPTKAVFEDAHLHVFNNGTESITVDYLSAWSMR 573
            GKT ITSRVYPTKAV E AHL VFNNG++ +TV+ L+AW+M+
Sbjct: 548 KGKTVITSRVYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNMQ 580

BLAST of Cp4.1LG12g04220 vs. TrEMBL
Match: A0A0A0LAY1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G777580 PE=3 SV=1)

HSP 1 Score: 980.3 bits (2533), Expect = 9.9e-283
Identity = 460/578 (79.58%), Postives = 512/578 (88.58%), Query Frame = 1

Query: 1   MNFFTLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQP 60
           MN FT L++LL FSF SLF++NG V V ALHK YP+LQS++  + + IK LHRT FHFQP
Sbjct: 1   MNLFTFLSLLLGFSFFSLFINNGVVFVDALHKIYPELQSIQT-DASVIKPLHRTRFHFQP 60

Query: 61  HRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPF 120
            R+W+NDPNGPMYFKGIYHLFYQYNP+GAVWGNIVWAHSIS+DLINWK LKPA+ PSKPF
Sbjct: 61  RRNWINDPNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKPAIYPSKPF 120

Query: 121 DINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDP 180
           DINGCWSGSATVLPG KPVILYTGID QNRQVQNYAIPANLSDP+LTEW+KPDNNPIVDP
Sbjct: 121 DINGCWSGSATVLPGDKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDNNPIVDP 180

Query: 181 GTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPN 240
           G  VN+SAFRDP+TAWLS +GHWKT+IGS+ ++ GMAYLYRS+DF+ WTK + PLHS  N
Sbjct: 181 GPGVNASAFRDPTTAWLSKSGHWKTVIGSKRKKRGMAYLYRSRDFVKWTKAKHPLHSAAN 240

Query: 241 TGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPD 300
           TGMWECPDFYPV   G+ GLD SVTGNWVKHV KVSL++TRYEYYTVGKY+P  DKYVPD
Sbjct: 241 TGMWECPDFYPVPLRGKLGLDPSVTGNWVKHVFKVSLDLTRYEYYTVGKYFPKKDKYVPD 300

Query: 301 NTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRT 360
           NTS DGWSGLRYDYGNFYASKSFYDP+KKRRVLWGWANESDS QDDVSKGWAGIQ IPRT
Sbjct: 301 NTSVDGWSGLRYDYGNFYASKSFYDPLKKRRVLWGWANESDSAQDDVSKGWAGIQLIPRT 360

Query: 361 VWLDPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSL 420
           VWLD N +QL QWP+ ELN LR K V++ +QK++KG  VE+KGITAAQADVEV+FSFSSL
Sbjct: 361 VWLDHNQRQLKQWPVKELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVMFSFSSL 420

Query: 421 DNAETFDPSWVDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVV 480
           D A+ FDP WVDAQA C QLG+ +QGGVGPFGLLTLASENL+EFTPVFFRIFKA   HVV
Sbjct: 421 DKADPFDPRWVDAQAACDQLGN-SQGGVGPFGLLTLASENLDEFTPVFFRIFKAHHKHVV 480

Query: 481 LMCSDARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRV 540
           LMCSDARSSSLK +GLYKP FAG+VDIDLS KKL LRSLIDHSV+ESFGGGGKTCITSRV
Sbjct: 481 LMCSDARSSSLKEEGLYKPAFAGYVDIDLSRKKLSLRSLIDHSVVESFGGGGKTCITSRV 540

Query: 541 YPTKAVFEDAHLHVFNNGTESITVDYLSAWSMRSAWVN 578
           YPTK VF+DAHLHVFNNGTE+ITV+YL AWSM+SA +N
Sbjct: 541 YPTKGVFDDAHLHVFNNGTEAITVEYLRAWSMKSARMN 576

BLAST of Cp4.1LG12g04220 vs. TrEMBL
Match: A0A059DIE8_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02647 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 3.8e-242
Identity = 393/576 (68.23%), Postives = 466/576 (80.90%), Query Frame = 1

Query: 4   FTLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRH 63
           F LL +++ F F      +    V+A HK YP+ QSL       +K  HRTG+HFQP +H
Sbjct: 15  FYLLLVIVAFDF-----GDENCAVEARHKVYPEYQSLPA---VSVKYAHRTGYHFQPPKH 74

Query: 64  WMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDIN 123
           W+NDPNGPM++KG YHLFYQYNP+GAVWGNIVWAHS+SKDL+NW++L PA+ P+KPFDIN
Sbjct: 75  WINDPNGPMHYKGWYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWRALPPAIYPTKPFDIN 134

Query: 124 GCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTE 183
           GCWSGSATVLPG +P+ILYTGID++N QVQNYA+PAN SDP+L EW KPDNNPIVDPG  
Sbjct: 135 GCWSGSATVLPGNRPIILYTGIDTKNHQVQNYAVPANASDPYLREWTKPDNNPIVDPGPG 194

Query: 184 VNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGM 243
           VN+SAFRDP+TAW   +GHWK ++GSR +  GMAYLYRS+DF++WTK + PLHS P TGM
Sbjct: 195 VNASAFRDPTTAWFE-DGHWKILVGSRRKHRGMAYLYRSRDFIHWTKAKHPLHSAPKTGM 254

Query: 244 WECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTS 303
           WECPDF+PVS     GLDTS  G  VKHVLKVSL++TRYEYYT+G Y+P  D+Y+PDNTS
Sbjct: 255 WECPDFFPVSKNNANGLDTSALGQGVKHVLKVSLDLTRYEYYTIGTYHPDKDRYMPDNTS 314

Query: 304 ADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWL 363
           ADGWSGLRYDYGNFYASK+F+DP+K RR+LWGWANESD+VQ DV KGWAGIQ IPR +WL
Sbjct: 315 ADGWSGLRYDYGNFYASKTFFDPVKNRRLLWGWANESDAVQSDVQKGWAGIQTIPRKIWL 374

Query: 364 DPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSLDNA 423
           D  GKQLLQWP+ EL  LR + V M + K+  G++VEIKGITAAQADV+V+FSFSSLD A
Sbjct: 375 DSYGKQLLQWPVEELETLRGQNVHMSSVKLNNGEHVEIKGITAAQADVDVVFSFSSLDGA 434

Query: 424 ETFDPSWVDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKA--ETNHVVL 483
           E FD SW++AQ +C   GS   G VGPFGLLTLASE+LEEFTPVFFRIFKA  +  H VL
Sbjct: 435 EQFDSSWLNAQDLCGLKGSGVPGNVGPFGLLTLASEHLEEFTPVFFRIFKADDQNKHKVL 494

Query: 484 MCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYP 543
           MCSDARSSSLK G+YKP+FAGFVD+DLS  K+ LRSLIDHSV+ESFG GGKTCITSRVYP
Sbjct: 495 MCSDARSSSLKSGIYKPSFAGFVDVDLSDGKISLRSLIDHSVVESFGAGGKTCITSRVYP 554

Query: 544 TKAVFEDAHLHVFNNGTESITVDYLSAWSMRSAWVN 578
           T A+  +A L  FNNGT++IT++ L AWSM    +N
Sbjct: 555 TLALLGNARLFTFNNGTQTITIETLDAWSMNRPVMN 581

BLAST of Cp4.1LG12g04220 vs. TrEMBL
Match: A0A061EUI0_THECC (Cell wall invertase 2 isoform 1 OS=Theobroma cacao GN=TCM_022641 PE=3 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 4.0e-239
Identity = 383/572 (66.96%), Postives = 467/572 (81.64%), Query Frame = 1

Query: 7   LAILLQFSFCSLF-VDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHWM 66
           L IL+ F    +  ++NG V   A HK +P+ QSL   N   ++Q+HRTG+HFQP ++W+
Sbjct: 9   LCILITFLCVDIINLNNGGV--DASHKVFPQFQSL---NAVSVQQIHRTGYHFQPPKNWI 68

Query: 67  NDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGC 126
           NDPN PMY+ GIYH FYQYNP+GAVWGNIVWAHS+SKD+INW++L+PAL PS+ FDINGC
Sbjct: 69  NDPNAPMYYDGIYHFFYQYNPKGAVWGNIVWAHSVSKDMINWQALEPALYPSQHFDINGC 128

Query: 127 WSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVN 186
           WSGSATVLP  KP+ILYTG+D ++RQ+QNYA+PANLSDP+L +W+KP NNP+VDP   VN
Sbjct: 129 WSGSATVLPSNKPIILYTGLDPKDRQIQNYAVPANLSDPYLRKWIKPGNNPVVDPEQGVN 188

Query: 187 SSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWE 246
           +SAFRDP+TAW  LNGHW+ ++GS+    GMAYLYRS+DF+ WTK + PLHS P+TGMWE
Sbjct: 189 ASAFRDPTTAWW-LNGHWRMLVGSKRNHRGMAYLYRSRDFIYWTKAKHPLHSAPHTGMWE 248

Query: 247 CPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSAD 306
           CPDFYPV   G+ GLD SV G  VKHVLKVSL++TRY+YYTVG Y    D+YVPDNTS D
Sbjct: 249 CPDFYPVLLSGKKGLDPSVLGKHVKHVLKVSLDVTRYDYYTVGTYLSDKDRYVPDNTSVD 308

Query: 307 GWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDP 366
           GW+GLRYDYGNFYASK+F+DP KKRR+LWGW NESD+ +DDV KGWAG+QAIPR VWLDP
Sbjct: 309 GWAGLRYDYGNFYASKTFFDPAKKRRILWGWVNESDTTEDDVKKGWAGLQAIPRIVWLDP 368

Query: 367 NGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSLDNAET 426
           N +QL+QWPI EL  LR K V M +Q++ KG+++E+KGITAAQADV+V F   +LD AE 
Sbjct: 369 NQRQLMQWPIEELETLRGKNVKMSHQQLKKGEHIEVKGITAAQADVDVTFHIPNLDKAEP 428

Query: 427 FDPSWVDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCSD 486
           FD SW DAQ +C + GS  +G VGPFGLLTLAS+NLEE+TPV FR+F+    HVVL+CSD
Sbjct: 429 FDHSWTDAQELCVKKGSKVKGEVGPFGLLTLASKNLEEYTPVSFRVFQTSKKHVVLLCSD 488

Query: 487 ARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAV 546
           ARSSSLKDGLYKP+FAGFVD+DL+ KKL LRSLIDHSV+ESFG GGKTCITSRVYPT AV
Sbjct: 489 ARSSSLKDGLYKPSFAGFVDVDLADKKLSLRSLIDHSVVESFGAGGKTCITSRVYPTLAV 548

Query: 547 FEDAHLHVFNNGTESITVDYLSAWSMRSAWVN 578
            ++AHL+ FN+GTE I V+ L+AWSM+   +N
Sbjct: 549 LDNAHLYAFNHGTEPINVENLNAWSMKKPRMN 574

BLAST of Cp4.1LG12g04220 vs. TrEMBL
Match: A0A0N6XFM9_RHUCH (Cell wall invertase OS=Rhus chinensis PE=2 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 4.0e-239
Identity = 390/573 (68.06%), Postives = 464/573 (80.98%), Query Frame = 1

Query: 7   LAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHWMN 66
           LAI L  +   L  ++G   ++A H  YP+ QSL       +KQ HRTGFHFQP +HW+N
Sbjct: 7   LAISLFLASLFLITNHG---IEASHVVYPEFQSLAA---VTVKQTHRTGFHFQPPKHWIN 66

Query: 67  DPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCW 126
           DPNGPMY+ GIYH FYQYNP+GAVWGNIVWAHS+S+D+INW++L+PA+ PSKPFDI GCW
Sbjct: 67  DPNGPMYYNGIYHFFYQYNPKGAVWGNIVWAHSVSRDMINWQALEPAIYPSKPFDIKGCW 126

Query: 127 SGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNS 186
           SGS T+LPG KP+ILYTGIDS+ RQ+Q+YA+P NLSDP+L EWVKPDNNP+V P   VN+
Sbjct: 127 SGSVTILPGNKPIILYTGIDSEERQLQDYAVPKNLSDPYLREWVKPDNNPLVFPHG-VNA 186

Query: 187 SAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWEC 246
           SAFRDP+TAW   +GHW+ ++GSR +Q G+AYLYRSKDF+ WTK + P+HS   TGMWEC
Sbjct: 187 SAFRDPTTAWWEDDGHWRMLVGSRRKQRGIAYLYRSKDFMKWTKAKHPIHSKEKTGMWEC 246

Query: 247 PDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADG 306
           PDF+PVS  G  GLDTS  G  +K+V K+SL+MTRY+YYTVG Y+   D+YVPDNTS DG
Sbjct: 247 PDFFPVSLSGTNGLDTSYRGPNLKYVFKISLDMTRYDYYTVGSYFRDKDRYVPDNTSVDG 306

Query: 307 WSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPN 366
           W GLRYDYGNFYASK+F+D  K RR+LWGWANESDS  DDV KGWAG+QAIPR++WLDP+
Sbjct: 307 WDGLRYDYGNFYASKTFFDAGKNRRILWGWANESDSKDDDVDKGWAGVQAIPRSLWLDPS 366

Query: 367 GKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEI-KGITAAQADVEVIFSFSSLDNAET 426
           GKQ+LQWPI EL  LR K   + N+ +  G+NVE+ +GITAAQADVEV FSF SLD AE 
Sbjct: 367 GKQVLQWPIQELETLRGKNAEISNKVLQSGENVEVGQGITAAQADVEVTFSFPSLDKAEP 426

Query: 427 FDPSWVDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCSD 486
           FDPSWV+A+ +C Q GST +GGVGPFGLLTLASE LEE+TPVFFRIFKA   HVVLMCSD
Sbjct: 427 FDPSWVNAEKLCGQKGSTVKGGVGPFGLLTLASEKLEEYTPVFFRIFKAPNKHVVLMCSD 486

Query: 487 ARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKA 546
           A SSSLK  GLYKP+FAGFVD+DL+ KKL LRSLIDHSV+ESFG GGKTCITSRVYPT A
Sbjct: 487 ATSSSLKTTGLYKPSFAGFVDVDLADKKLSLRSLIDHSVVESFGAGGKTCITSRVYPTLA 546

Query: 547 VFEDAHLHVFNNGTESITVDYLSAWSMRSAWVN 578
           VF +AHL  FNNGTE++T+D L+AWSM    +N
Sbjct: 547 VFSNAHLFAFNNGTETVTIDKLNAWSMNKPQMN 572

BLAST of Cp4.1LG12g04220 vs. TrEMBL
Match: A0A067FSV9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g008189mg PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 1.1e-238
Identity = 393/575 (68.35%), Postives = 469/575 (81.57%), Query Frame = 1

Query: 1   MNFFTLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQP 60
           ++ F L+A L   S      +NG   V+A HK YP+ QSL      ++KQLHRT FHFQP
Sbjct: 7   LSIFMLIAYLWVISN-----NNG---VEASHKIYPEFQSLAA---VKVKQLHRTAFHFQP 66

Query: 61  HRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPF 120
            +HW+N   GPMY+KGIYHLFYQYNP+GAVWGNIVWAHS+SKDLINW++L+PAL PSKPF
Sbjct: 67  PKHWIN---GPMYYKGIYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPALYPSKPF 126

Query: 121 DINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDP 180
           DINGCWSGSATVLPG KP+ILYTG+D + RQVQNYA+PAN SDP+L +W+KPDNNP+V P
Sbjct: 127 DINGCWSGSATVLPGNKPIILYTGVDHKERQVQNYAVPANPSDPYLRKWIKPDNNPVVFP 186

Query: 181 GTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPN 240
           G +VN+SAFRDP+TAW S +GHW+ ++GSR +  GMAYLYRS+DF+ WTK + P+HS+ N
Sbjct: 187 GPDVNASAFRDPTTAWWSNDGHWRMLVGSRRKHRGMAYLYRSRDFMKWTKAKHPIHSLAN 246

Query: 241 TGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPD 300
           TGMWECPDFYPVS  G+ GLDTS  G   K VLKVSL++TRY+YYT+G Y    D+YVPD
Sbjct: 247 TGMWECPDFYPVSISGKNGLDTSFAGGNEKFVLKVSLDLTRYDYYTIGTYNRDKDRYVPD 306

Query: 301 NTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRT 360
             S DGW GLRYDYGNFYASK+F+D  K RR+LWGWANESDS  DD++KGWAG+Q IPR 
Sbjct: 307 KDSVDGWRGLRYDYGNFYASKTFFDSRKNRRILWGWANESDSTFDDMAKGWAGVQTIPRE 366

Query: 361 VWLDPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSL 420
           VWLD +GKQLLQWPI EL  LR K V + NQ++ KG +VE+KG+TAAQADVEV F   SL
Sbjct: 367 VWLDGSGKQLLQWPIEELETLRGKNVHISNQELKKGHHVEVKGVTAAQADVEVTFKLPSL 426

Query: 421 DNAETFDPSW--VDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNH 480
           D AE FDPSW  +DA+ +C ++GS  QGGVGPFGLLTLAS+NLEEFTPVFFRIFK    H
Sbjct: 427 DKAEKFDPSWKNLDAEHLCGKIGSKVQGGVGPFGLLTLASKNLEEFTPVFFRIFKDGHKH 486

Query: 481 VVLMCSDARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITS 540
           +VLMCSDA SS+LK +GLYKP+FAGFV++DLS KKL LRSLIDHSV+ESFG GGKTCITS
Sbjct: 487 LVLMCSDASSSTLKTEGLYKPSFAGFVNVDLSDKKLSLRSLIDHSVVESFGAGGKTCITS 546

Query: 541 RVYPTKAVFEDAHLHVFNNGTESITVDYLSAWSMR 573
           RVYPT AVF+DAHL+ FNNGTE++TV+ L+AWSM+
Sbjct: 547 RVYPTLAVFDDAHLYAFNNGTETVTVEKLNAWSMK 567

BLAST of Cp4.1LG12g04220 vs. TAIR10
Match: AT2G36190.1 (AT2G36190.1 cell wall invertase 4)

HSP 1 Score: 719.5 bits (1856), Expect = 1.6e-207
Identity = 331/557 (59.43%), Postives = 424/557 (76.12%), Query Frame = 1

Query: 27  VQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYNP 86
           + A H+ Y +LQS    +   +  LHR  FHFQP +HW+NDPNGP+Y+KG+YHLFYQYN 
Sbjct: 27  IDAFHQIYEELQS---ESVESVNHLHRPSFHFQPPKHWINDPNGPVYYKGLYHLFYQYNT 86

Query: 87  EGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGID 146
           +GAVWGNI+WAHS+SKDL+NW++L+PALSPSK FDI G WSGS T++PG  P+ILYTG++
Sbjct: 87  KGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTGVN 146

Query: 147 SQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLSLNGHWKTI 206
               Q+QNYAIP + SDP+L +W+KPD+NPI  P   +N SAFRDP+TAW S +GHW+T+
Sbjct: 147 QNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTMNGSAFRDPTTAWFSKDGHWRTV 206

Query: 207 IGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDTSVT 266
           +GS+ ++ G+AY+YRS+DF +W K + P+HS  +TGMWECPDF+PVS    R GLD    
Sbjct: 207 VGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMWECPDFFPVSLTDFRNGLDLDYV 266

Query: 267 GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYD 326
           G   KHVLKVSL++TRYEYYT+GKY    D+Y+PD  + DGW GLR+DYGNFYASK+F+D
Sbjct: 267 GPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNTPDGWEGLRFDYGNFYASKTFFD 326

Query: 327 PMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRRKI 386
             K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV LD + KQL+ WP+ E+  LR   
Sbjct: 327 YKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLLDSSKKQLVFWPVEEIESLRGNY 386

Query: 387 VLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSLDNAETFDPSWV-DAQAVCSQLGSTT 446
           V M N  +  GQ +E+KGIT AQADVEV F   SL+ AE FDPS+      +C+  GS  
Sbjct: 387 VRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEIFDPSFTWKPLELCNIKGSNV 446

Query: 447 QGGVGPFGLLTLASENLEEFTPVFFRIFKAETNH--VVLMCSDARSSSL--------KDG 506
           +GGVGPFGL+TLA+ +LEE+TPVFFR+F     H   VLMCSDAR SSL        KD 
Sbjct: 447 RGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPKVLMCSDARPSSLKQDTGLLAKDR 506

Query: 507 LYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEDAHLHVF 566
           +YKP+FAGFVD+D++  ++ LRSLIDHSV+ESFG  GKT ITSRVYP KAV E+AHL+VF
Sbjct: 507 MYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGKTVITSRVYPVKAVKENAHLYVF 566

Query: 567 NNGTESITVDYLSAWSM 572
           NNGT+++T++ L+AW+M
Sbjct: 567 NNGTQTVTIESLNAWNM 580

BLAST of Cp4.1LG12g04220 vs. TAIR10
Match: AT3G52600.1 (AT3G52600.1 cell wall invertase 2)

HSP 1 Score: 703.7 bits (1815), Expect = 9.1e-203
Identity = 341/583 (58.49%), Postives = 427/583 (73.24%), Query Frame = 1

Query: 3   FFTLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHR 62
           +  LL +L+  S      +NG   V A HK + KLQS     ++ +  LHRT +HFQP R
Sbjct: 8   YVLLLIVLINIS------NNG---VDAFHKVFKKLQSKSTSLES-VSPLHRTAYHFQPPR 67

Query: 63  HWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDI 122
           HW+NDPN PM +KG+YHLFYQYNP+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FDI
Sbjct: 68  HWINDPNAPMLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDI 127

Query: 123 NGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGT 182
           NG WSGSAT +PG  PVILYTGI     Q+QNYAIP +LSDP+L  W+KPD+NPIV P  
Sbjct: 128 NGTWSGSATHVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDN 187

Query: 183 EVNSSAFRDPSTAWLS-LNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNT 242
             N SAFRDP+TAW +  +G+W+ ++GS+ +  G+AY+Y+S+DF  W K ++P+HS   T
Sbjct: 188 GENGSAFRDPTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKT 247

Query: 243 GMWECPDFYPVSHFG-RFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPD 302
           GMWECPDF+PVS    + GLD S  G   KHVLKVSL++TRYEYYT+G Y    D+Y PD
Sbjct: 248 GMWECPDFFPVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPD 307

Query: 303 NTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRT 362
             + DGW GLR+DYGN+YASK+F+D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT
Sbjct: 308 GYTPDGWDGLRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRT 367

Query: 363 VWLDPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSL 422
           + LD +GKQL+ WPI E+  LR K V M NQK+  GQ  E++GIT AQ DV+V F+  +L
Sbjct: 368 ILLDSSGKQLVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNL 427

Query: 423 DNAETFDPSWVDAQ-AVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFK--AETN 482
           + AE FD S+      +C+  GS   GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A   
Sbjct: 428 EKAEKFDESFATKPLELCNLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNK 487

Query: 483 HVVLMCSDARSSSL--------KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGG 542
             VLMCSDA+ SSL        K+ +YKP+FAGFVD+ L   K+ LRSLIDHSV+ESFG 
Sbjct: 488 PKVLMCSDAKPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGA 547

Query: 543 GGKTCITSRVYPTKAVFEDAHLHVFNNGTESITVDYLSAWSMR 573
            GKT ITSRVYPTKAV E AHL VFNNG++ +TV+ L+AW+M+
Sbjct: 548 KGKTVITSRVYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNMQ 580

BLAST of Cp4.1LG12g04220 vs. TAIR10
Match: AT3G13790.1 (AT3G13790.1 Glycosyl hydrolases family 32 protein)

HSP 1 Score: 672.5 bits (1734), Expect = 2.2e-193
Identity = 319/570 (55.96%), Postives = 409/570 (71.75%), Query Frame = 1

Query: 17  SLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHWMNDPNGPMYFKG 76
           +L + N  V ++A H  Y +L    N     + Q +RTGFHFQP ++WMNDPNGPM +KG
Sbjct: 16  TLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIYKG 75

Query: 77  IYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGL 136
           IYHLFYQ+NP+GAVWGNIVWAHS S DLINW    PA+ PS PFDINGCWSGSAT+LP  
Sbjct: 76  IYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPNG 135

Query: 137 KPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG--TEVNSSAFRDPST 196
           KPVILYTGID +N+QVQN A P NLSDP+L EW K   NP++ P     +N+S+FRDP+T
Sbjct: 136 KPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPTT 195

Query: 197 AWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYPVSH 256
           AWL  +  W+ IIGS+  + G+A  Y SKDFL W K  +PLH    +GMWECPDF+PV+ 
Sbjct: 196 AWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVTR 255

Query: 257 FGRFGLDTSVTG---NWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLR 316
           FG  G++TS  G     +KHVLK+SL+ T+++YYT+G Y  + DK+VPDN      +  R
Sbjct: 256 FGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAPR 315

Query: 317 YDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLL 376
           YDYG +YASK+F+D  K RR+LWGW NES SV+DDV KGW+GIQ IPR +WLD +GKQL+
Sbjct: 316 YDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLI 375

Query: 377 QWPIMELNRLRRKIVLMINQKVVK-GQNVEIKGITAAQADVEVIFSFSSLDNAETFDPSW 436
           QWP+ E+ RLR K V  +  KV+K G  +E+ G+TAAQADVEV+F    L+ A+  +PSW
Sbjct: 376 QWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPSW 435

Query: 437 VDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETN---HVVLMCSDAR 496
            D Q +CS++  + + G+GPFGL+ LAS+NLEE+T V+FRIFKA  N   +VVLMCSD  
Sbjct: 436 TDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQS 495

Query: 497 SSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFE 556
            SSLK+   K T+  FVDI+   + L LR+LIDHSV+ESFGG G+ CITSRVYP  A+ +
Sbjct: 496 RSSLKEDNDKTTYGAFVDIN-PHQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIGK 555

Query: 557 DAHLHVFNNGTESITVDYLSAWSMRSAWVN 578
            +HL  FN G +S+ V  L+AWSM SA ++
Sbjct: 556 SSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of Cp4.1LG12g04220 vs. TAIR10
Match: AT1G55120.1 (AT1G55120.1 beta-fructofuranosidase 5)

HSP 1 Score: 638.3 bits (1645), Expect = 4.7e-183
Identity = 301/552 (54.53%), Postives = 389/552 (70.47%), Query Frame = 1

Query: 37  LQSLENGNDTEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWG-NIV 96
           +  LE  +  ++ Q +RTG+HFQP ++WMNDPNGPM +KGIYHLFYQYNP GAVW   IV
Sbjct: 23  ITDLEASSHQDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIV 82

Query: 97  WAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNY 156
           W HS S DL+NW S  PA +PS+P DINGCWSGS T+LP  KPVILYTGID    QVQN 
Sbjct: 83  WGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNV 142

Query: 157 AIPANLSDPHLTEWVKPDNNPIVDPGTE--VNSSAFRDPSTAWLSLNGHWKTIIGSRSE- 216
           A+P N+SDP+L EW KP  NP++       +N   FRDP+TAWL  +G W+ I+GS ++ 
Sbjct: 143 AVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDD 202

Query: 217 QTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFGRFGLDTSVTG-NWVKH 276
           + G+A LY+S+DF NWT+  +PLH    TGMWECPDF+PVS  G  G++TS  G N +KH
Sbjct: 203 RRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKH 262

Query: 277 VLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYDPMKKRR 336
           VLKVSL  T ++YYT+G Y    D YVPD       S  R DYG +YASK+FYD +KKRR
Sbjct: 263 VLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRR 322

Query: 337 VLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRRKIVLMINQ 396
           +LWGW NES   +DD+ KGW+G+Q+ PR +WLD +GK+LLQWPI E+  LR + V    +
Sbjct: 323 ILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKK 382

Query: 397 KVVKGQNVEIKGITAAQADVEVIFSFSSLDNAETFDPSWVDAQAVCSQLGSTTQGGVGPF 456
            +  G  +++ G+TAAQADVEV F    L+ A+  +PSW D Q +CSQ   +   G+GPF
Sbjct: 383 VLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQGDLSVMSGLGPF 442

Query: 457 GLLTLASENLEEFTPVFFRIFKA------ETNHVVLMCSDARSSSLKDGLYKPTFAGFVD 516
           GL+ LAS ++EE+T V+FRIFK+      +T +VVLMCSD   SSL D   K TF  FV 
Sbjct: 443 GLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVA 502

Query: 517 IDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEDAHLHVFNNGTESITVDY 576
           ID S + + LR+LIDHS++ES+GGGG+TCITSRVYP  A+ E+A+L VFN GT+S+ +  
Sbjct: 503 IDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILT 562

Query: 577 LSAWSMRSAWVN 578
           LSAWS++SA +N
Sbjct: 563 LSAWSLKSAQIN 574

BLAST of Cp4.1LG12g04220 vs. TAIR10
Match: AT3G13784.1 (AT3G13784.1 cell wall invertase 5)

HSP 1 Score: 589.0 bits (1517), Expect = 3.3e-168
Identity = 297/575 (51.65%), Postives = 383/575 (66.61%), Query Frame = 1

Query: 10  LLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHWMNDPN 69
           +  F   SLF+ +  VVV         L +L+N  +    Q +RTG+HFQP ++WMN   
Sbjct: 9   IAMFLLVSLFLTDDAVVV---------LDALDNVPNNIKNQPYRTGYHFQPPKNWMN--- 68

Query: 70  GPMYFKGIYHLFYQYNPEGAVW--GNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWS 129
           GPM +KGIYHLFYQ+N  GAV      VW H+ S DLINW +L PA+ PS+P DINGCWS
Sbjct: 69  GPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINWITLSPAIRPSRPSDINGCWS 128

Query: 130 GSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG--TEVN 189
           GS T+LP  KPVILYTG D  NRQVQN   P NL+DP+L  W K   NP+V P     +N
Sbjct: 129 GSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLRHWTKSPENPLVTPSPVNHIN 188

Query: 190 SSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWE 249
           SSAFRDP+TAW   +G W+   GS+  + G+A L+ SKDF+ W +  +PLH    TGMWE
Sbjct: 189 SSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDFVIWKQSPKPLHYHDGTGMWE 248

Query: 250 CPDFYPVSHFGRFGLDTSVT-GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSA 309
           CPDF+PV+     GLDTS + G  VKHVLKVSL  T  +YYT+G Y  + D YVPD    
Sbjct: 249 CPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFNDYYTIGTYDEVRDVYVPDKGFV 308

Query: 310 DGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLD 369
              +  RYDYG FYASK+FYD + +RR+LWGW NES   +D++ KGWAG+QAIPR VWLD
Sbjct: 309 QDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPEKDNIKKGWAGLQAIPRKVWLD 368

Query: 370 PNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFS--SLDN 429
            +GK+L+QWP+ E+ RLR   V   N+ +  G  +E+ G+TA QADVEV F  S   L+ 
Sbjct: 369 ESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHGVTAPQADVEVFFKVSGFDLEK 428

Query: 430 AETFDPSWVDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETN---HV 489
           A+  +P W D Q +CSQ  +++  G+GPFGL+ LAS+N+EE+T V  RIF+A  N   HV
Sbjct: 429 ADVIEPGWTDPQLICSQRNASS--GLGPFGLMVLASKNMEEYTSVNIRIFRAGENSKEHV 488

Query: 490 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRV 549
           V+MCSD  +SSL+ G  K T+  F+DI    + + LR+LID S++ESFGG GKTCITSRV
Sbjct: 489 VVMCSDQSTSSLEKGNDKTTYGAFLDIS-PYQPISLRTLIDKSIVESFGGKGKTCITSRV 548

Query: 550 YPTKAVFEDAHLHVFNNGTESITVDYLSAWSMRSA 575
           YP  A+ E  HL  FN G++++ V  LSAWSM+S+
Sbjct: 549 YPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568

BLAST of Cp4.1LG12g04220 vs. NCBI nr
Match: gi|449462946|ref|XP_004149196.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucumis sativus])

HSP 1 Score: 980.3 bits (2533), Expect = 1.4e-282
Identity = 460/578 (79.58%), Postives = 512/578 (88.58%), Query Frame = 1

Query: 1   MNFFTLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQP 60
           MN FT L++LL FSF SLF++NG V V ALHK YP+LQS++  + + IK LHRT FHFQP
Sbjct: 1   MNLFTFLSLLLGFSFFSLFINNGVVFVDALHKIYPELQSIQT-DASVIKPLHRTRFHFQP 60

Query: 61  HRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPF 120
            R+W+NDPNGPMYFKGIYHLFYQYNP+GAVWGNIVWAHSIS+DLINWK LKPA+ PSKPF
Sbjct: 61  RRNWINDPNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKPAIYPSKPF 120

Query: 121 DINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDP 180
           DINGCWSGSATVLPG KPVILYTGID QNRQVQNYAIPANLSDP+LTEW+KPDNNPIVDP
Sbjct: 121 DINGCWSGSATVLPGDKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDNNPIVDP 180

Query: 181 GTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPN 240
           G  VN+SAFRDP+TAWLS +GHWKT+IGS+ ++ GMAYLYRS+DF+ WTK + PLHS  N
Sbjct: 181 GPGVNASAFRDPTTAWLSKSGHWKTVIGSKRKKRGMAYLYRSRDFVKWTKAKHPLHSAAN 240

Query: 241 TGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPD 300
           TGMWECPDFYPV   G+ GLD SVTGNWVKHV KVSL++TRYEYYTVGKY+P  DKYVPD
Sbjct: 241 TGMWECPDFYPVPLRGKLGLDPSVTGNWVKHVFKVSLDLTRYEYYTVGKYFPKKDKYVPD 300

Query: 301 NTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRT 360
           NTS DGWSGLRYDYGNFYASKSFYDP+KKRRVLWGWANESDS QDDVSKGWAGIQ IPRT
Sbjct: 301 NTSVDGWSGLRYDYGNFYASKSFYDPLKKRRVLWGWANESDSAQDDVSKGWAGIQLIPRT 360

Query: 361 VWLDPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSL 420
           VWLD N +QL QWP+ ELN LR K V++ +QK++KG  VE+KGITAAQADVEV+FSFSSL
Sbjct: 361 VWLDHNQRQLKQWPVKELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVMFSFSSL 420

Query: 421 DNAETFDPSWVDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVV 480
           D A+ FDP WVDAQA C QLG+ +QGGVGPFGLLTLASENL+EFTPVFFRIFKA   HVV
Sbjct: 421 DKADPFDPRWVDAQAACDQLGN-SQGGVGPFGLLTLASENLDEFTPVFFRIFKAHHKHVV 480

Query: 481 LMCSDARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRV 540
           LMCSDARSSSLK +GLYKP FAG+VDIDLS KKL LRSLIDHSV+ESFGGGGKTCITSRV
Sbjct: 481 LMCSDARSSSLKEEGLYKPAFAGYVDIDLSRKKLSLRSLIDHSVVESFGGGGKTCITSRV 540

Query: 541 YPTKAVFEDAHLHVFNNGTESITVDYLSAWSMRSAWVN 578
           YPTK VF+DAHLHVFNNGTE+ITV+YL AWSM+SA +N
Sbjct: 541 YPTKGVFDDAHLHVFNNGTEAITVEYLRAWSMKSARMN 576

BLAST of Cp4.1LG12g04220 vs. NCBI nr
Match: gi|659084391|ref|XP_008442862.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucumis melo])

HSP 1 Score: 979.2 bits (2530), Expect = 3.2e-282
Identity = 462/574 (80.49%), Postives = 509/574 (88.68%), Query Frame = 1

Query: 5   TLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRHW 64
           T L++LL FSF SLF++NG   V+ALHK YP+LQS++  + + +K LHRT FHFQP R+W
Sbjct: 6   TFLSLLLGFSFFSLFINNG---VEALHKIYPELQSIQT-DASVVKPLHRTRFHFQPRRNW 65

Query: 65  MNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDING 124
           +NDPNGPMYF GIYHLFYQYNP+GAVWGNIVWAHSIS+DLINWK LK A+ PSKPFDING
Sbjct: 66  INDPNGPMYFNGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKAAIYPSKPFDING 125

Query: 125 CWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEV 184
           CWSGSATVLPG KPVILYTGID QNRQVQNYAIPANLSDP+LTEW+KPD+NPIVDPG  V
Sbjct: 126 CWSGSATVLPGNKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDHNPIVDPGPGV 185

Query: 185 NSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMW 244
           N+SAFRDP+TAWLS +GHWKTIIGS+ ++ GMAYLYRS+DF+ WTK + PLHS  NTGMW
Sbjct: 186 NASAFRDPTTAWLSKSGHWKTIIGSKRKRRGMAYLYRSRDFVKWTKAKHPLHSAANTGMW 245

Query: 245 ECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSA 304
           ECPDFYPV   GR GLD SVTGNWVKHVLKVSL++TRYEYYTVGKY+P  DKYVPDNTS 
Sbjct: 246 ECPDFYPVPLRGRLGLDPSVTGNWVKHVLKVSLDLTRYEYYTVGKYFPKKDKYVPDNTSV 305

Query: 305 DGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLD 364
           DGWSGLRYDYGNFYASKSFYDP++KRRVLWGWANESDS QDDVSKGWAGIQ IPRTVWLD
Sbjct: 306 DGWSGLRYDYGNFYASKSFYDPLRKRRVLWGWANESDSAQDDVSKGWAGIQLIPRTVWLD 365

Query: 365 PNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSLDNAE 424
            N KQL+QWP+ ELN LR K V++ +QK++KG  VE+KGITAAQADVEVIFSFSSLD AE
Sbjct: 366 HNQKQLIQWPVEELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVIFSFSSLDKAE 425

Query: 425 TFDPSWVDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCS 484
            FDP WVDAQA C QLG  +QGGVGPFGLLTLASENLEEFTPVFFRIFKA   HVVLMCS
Sbjct: 426 PFDPRWVDAQAACDQLGLKSQGGVGPFGLLTLASENLEEFTPVFFRIFKAHHKHVVLMCS 485

Query: 485 DARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTK 544
           DARSSSLK DGLYKP FAG+VDIDLSSKKL LRSLIDHSV+ESFGGGGKTCITSRVYPTK
Sbjct: 486 DARSSSLKEDGLYKPAFAGYVDIDLSSKKLSLRSLIDHSVVESFGGGGKTCITSRVYPTK 545

Query: 545 AVFEDAHLHVFNNGTESITVDYLSAWSMRSAWVN 578
            VF DAHLHVFNNGTE+ITV+YLSAWSM+SA VN
Sbjct: 546 GVFNDAHLHVFNNGTEAITVEYLSAWSMKSARVN 575

BLAST of Cp4.1LG12g04220 vs. NCBI nr
Match: gi|657989615|ref|XP_008387018.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like isoform X1 [Malus domestica])

HSP 1 Score: 847.8 bits (2189), Expect = 1.1e-242
Identity = 394/578 (68.17%), Postives = 469/578 (81.14%), Query Frame = 1

Query: 5   TLLAILLQFSF--CSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHR 64
           T + +L++  F  C +F  N    V+ALH  YP+ QSL      ++KQ+HRT FHFQP R
Sbjct: 5   TAIKLLVEVIFLACCVFAINNITGVEALHNVYPEFQSLAA---VKVKQIHRTAFHFQPPR 64

Query: 65  HWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDI 124
           +W+NDPNGPMYF G+YHLFYQYNPEGAVWGNIVWAHS+SKDLINW++L+PA+ PSKPFD+
Sbjct: 65  NWINDPNGPMYFGGLYHLFYQYNPEGAVWGNIVWAHSVSKDLINWEALEPAIYPSKPFDV 124

Query: 125 NGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGT 184
           NGCWSGSAT LPG KP++LYTG+DSQNRQVQNYAIP N SDP+L +W+KPDNNP+V P T
Sbjct: 125 NGCWSGSATXLPGNKPIMLYTGVDSQNRQVQNYAIPKNASDPYLRKWIKPDNNPLVIPDT 184

Query: 185 EVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTG 244
            +N++AFRDP+TAW   NGHWK ++G + +  GMA+LYRSKDF++W K   PLHS P TG
Sbjct: 185 GINATAFRDPTTAWWG-NGHWKMLVGGKRKHRGMAHLYRSKDFVHWVKAHHPLHSAPQTG 244

Query: 245 MWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNT 304
           MWECPDFYPV   G+ GLDTS  G  VKHVLKVSL+ TRYEYYTVGKY+P  D YVPD  
Sbjct: 245 MWECPDFYPVPLVGKLGLDTSKVGGDVKHVLKVSLDETRYEYYTVGKYFPEKDTYVPDKE 304

Query: 305 SADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVW 364
             DGWSGLRYDYGNFYASK+F+DP KKRR+LWGWANESD+  +DV+KGWAGIQ IPR VW
Sbjct: 305 LVDGWSGLRYDYGNFYASKTFFDPAKKRRILWGWANESDTASNDVAKGWAGIQTIPRDVW 364

Query: 365 LDPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSLDN 424
           L P+GKQLLQWP++EL  LR K V + NQ + +G +VE+KG+TAAQADVEVIFS  SL  
Sbjct: 365 LSPDGKQLLQWPVVELETLRGKKVGINNQILQQGSHVEVKGLTAAQADVEVIFSLPSLAK 424

Query: 425 AETFDPSW--VDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVV 484
           AE F+PSW  +DAQ +C + GS  QGG+GPFGLLTLASENLEEFTPVFFR+FKA+  HVV
Sbjct: 425 AEEFNPSWSNLDAQTLCGKKGSKIQGGIGPFGLLTLASENLEEFTPVFFRVFKAKGRHVV 484

Query: 485 LMCSDARSSSLKDGLYKPTFAGFVDIDLS-SKKLPLRSLIDHSVIESFGGGGKTCITSRV 544
           LMCS A SSSL+D LY+P+FAGFVD+DLS SKKL LRSLIDHSV+ES+G GGKTCITSRV
Sbjct: 485 LMCSXATSSSLQDNLYRPSFAGFVDVDLSHSKKLSLRSLIDHSVVESYGAGGKTCITSRV 544

Query: 545 YPTKAVFEDAHLHVFNNGTESITVDYLSAWSMRSAWVN 578
           YPT AV   AHL+VFNNGT+ +T + L+AWSM +  +N
Sbjct: 545 YPTLAVNNGAHLYVFNNGTQPVTAESLNAWSMNAPTMN 578

BLAST of Cp4.1LG12g04220 vs. NCBI nr
Match: gi|568856346|ref|XP_006481745.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like [Citrus sinensis])

HSP 1 Score: 846.7 bits (2186), Expect = 2.5e-242
Identity = 396/575 (68.87%), Postives = 472/575 (82.09%), Query Frame = 1

Query: 1   MNFFTLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQP 60
           ++ F L+A L   S      +NG   V+A HK YP+ QSL      ++KQLHRT FHFQP
Sbjct: 7   LSIFMLIAYLWVISN-----NNG---VEASHKIYPEFQSLAA---VKVKQLHRTAFHFQP 66

Query: 61  HRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPF 120
            +HW+NDPNGPMY+KGIYHLFYQYNP+GAVWGNIVWAHS+SKDLINW++L+PAL PSKPF
Sbjct: 67  PKHWINDPNGPMYYKGIYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPALYPSKPF 126

Query: 121 DINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDP 180
           DINGCWSGSATVLPG KP+ILYTG+D + RQVQNYA+PAN SDP+L +W+KPDNNP+V P
Sbjct: 127 DINGCWSGSATVLPGNKPIILYTGVDHKERQVQNYAVPANPSDPYLRKWIKPDNNPVVFP 186

Query: 181 GTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPN 240
           G +VN+SAFRDP+TAW S +GHW+ ++GSR +  GMAYLYRS+DF+ WTK + P+HS+ N
Sbjct: 187 GPDVNASAFRDPTTAWWSNDGHWRMLVGSRRKHRGMAYLYRSRDFMKWTKAKHPIHSLAN 246

Query: 241 TGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPD 300
           TGMWECPDFYPVS  G+ GLDTS  G   K VLKVSL++TRY+YYT+G Y    D+YVPD
Sbjct: 247 TGMWECPDFYPVSISGKNGLDTSFAGGNEKFVLKVSLDLTRYDYYTIGTYNRDKDRYVPD 306

Query: 301 NTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRT 360
             S DGW GLRYDYGNFYASK+F+D  K RR+LWGWANESDS  DD++KGWAG+Q IPR 
Sbjct: 307 KDSVDGWRGLRYDYGNFYASKTFFDSRKNRRILWGWANESDSTFDDMAKGWAGVQTIPRE 366

Query: 361 VWLDPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSL 420
           VWLD +GKQLLQWPI EL  LR K V + NQ++ KG +VE+KG+TAAQADVEV F   SL
Sbjct: 367 VWLDGSGKQLLQWPIEELETLRGKNVHISNQELKKGHHVEVKGVTAAQADVEVTFKLPSL 426

Query: 421 DNAETFDPSW--VDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNH 480
           D AE FDPSW  +DA+ +C ++GS  QGGVGPFGLLTLAS+NLEEFTPVFFRIFK    H
Sbjct: 427 DKAEKFDPSWKNLDAEHLCGKIGSKVQGGVGPFGLLTLASKNLEEFTPVFFRIFKDGHKH 486

Query: 481 VVLMCSDARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITS 540
           +VLMCSDA SS+LK +GLYKP+FAGFV++DLS KKL LRSLIDHSV+ESFG GGKTCITS
Sbjct: 487 LVLMCSDASSSTLKTEGLYKPSFAGFVNVDLSDKKLSLRSLIDHSVVESFGAGGKTCITS 546

Query: 541 RVYPTKAVFEDAHLHVFNNGTESITVDYLSAWSMR 573
           RVYPT AVF+DAHL+ FNNGTE++TV+ L+AWSM+
Sbjct: 547 RVYPTLAVFDDAHLYAFNNGTETVTVEKLNAWSMK 570

BLAST of Cp4.1LG12g04220 vs. NCBI nr
Match: gi|702249497|ref|XP_010059629.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 3-like [Eucalyptus grandis])

HSP 1 Score: 845.5 bits (2183), Expect = 5.5e-242
Identity = 393/576 (68.23%), Postives = 466/576 (80.90%), Query Frame = 1

Query: 4   FTLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGNDTEIKQLHRTGFHFQPHRH 63
           F LL +++ F F      +    V+A HK YP+ QSL       +K  HRTG+HFQP +H
Sbjct: 15  FYLLLVIVAFDF-----GDENCAVEARHKVYPEYQSLPA---VSVKYAHRTGYHFQPPKH 74

Query: 64  WMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDIN 123
           W+NDPNGPM++KG YHLFYQYNP+GAVWGNIVWAHS+SKDL+NW++L PA+ P+KPFDIN
Sbjct: 75  WINDPNGPMHYKGWYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWRALPPAIYPTKPFDIN 134

Query: 124 GCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTE 183
           GCWSGSATVLPG +P+ILYTGID++N QVQNYA+PAN SDP+L EW KPDNNPIVDPG  
Sbjct: 135 GCWSGSATVLPGNRPIILYTGIDTKNHQVQNYAVPANASDPYLREWTKPDNNPIVDPGPG 194

Query: 184 VNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGM 243
           VN+SAFRDP+TAW   +GHWK ++GSR +  GMAYLYRS+DF++WTK + PLHS P TGM
Sbjct: 195 VNASAFRDPTTAWFE-DGHWKILVGSRRKHRGMAYLYRSRDFIHWTKAKHPLHSAPKTGM 254

Query: 244 WECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTS 303
           WECPDF+PVS     GLDTS  G  VKHVLKVSL++TRYEYYT+G Y+P  D+Y+PDNTS
Sbjct: 255 WECPDFFPVSKNNANGLDTSALGQGVKHVLKVSLDLTRYEYYTIGTYHPDKDRYMPDNTS 314

Query: 304 ADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWL 363
           ADGWSGLRYDYGNFYASK+F+DP+K RR+LWGWANESD+VQ DV KGWAGIQ IPR +WL
Sbjct: 315 ADGWSGLRYDYGNFYASKTFFDPVKNRRLLWGWANESDAVQSDVQKGWAGIQTIPRKIWL 374

Query: 364 DPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSSLDNA 423
           D  GKQLLQWP+ EL  LR + V M + K+  G++VEIKGITAAQADV+V+FSFSSLD A
Sbjct: 375 DSYGKQLLQWPVEELETLRGQNVHMSSVKLNNGEHVEIKGITAAQADVDVVFSFSSLDGA 434

Query: 424 ETFDPSWVDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKA--ETNHVVL 483
           E FD SW++AQ +C   GS   G VGPFGLLTLASE+LEEFTPVFFRIFKA  +  H VL
Sbjct: 435 EQFDSSWLNAQDLCGLKGSGVPGNVGPFGLLTLASEHLEEFTPVFFRIFKADDQNKHKVL 494

Query: 484 MCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYP 543
           MCSDARSSSLK G+YKP+FAGFVD+DLS  K+ LRSLIDHSV+ESFG GGKTCITSRVYP
Sbjct: 495 MCSDARSSSLKSGIYKPSFAGFVDVDLSDGKISLRSLIDHSVVESFGAGGKTCITSRVYP 554

Query: 544 TKAVFEDAHLHVFNNGTESITVDYLSAWSMRSAWVN 578
           T A+  +A L  FNNGT++IT++ L AWSM    +N
Sbjct: 555 TLALLGNARLFTFNNGTQTITIETLDAWSMNRPVMN 581