Cp4.1LG12g04200 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG12g04200 vs. Swiss-Prot
Match: INV1_DAUCA (Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota GN=INV1 PE=1 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 8.2e-230
Identity = 385/576 (66.84%), Postives = 461/576 (80.03%), Query Frame = 1

Query: 5   TLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRH 64
           +LLAILL  +  +L I+     V+A H+I+  LQS+   N    +KQ+HRTG+HFQP ++
Sbjct: 20  SLLAILL-VTTTTLHING----VEAFHEIHYNLQSVGAEN----VKQVHRTGYHFQPKQN 79

Query: 65  WMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDIN 124
           W+NDPNGPMY+KG+YHLFYQYNP+GAVWGNIVWAHS+S DLINW  L+PA+ PSKPFD  
Sbjct: 80  WINDPNGPMYYKGVYHLFYQYNPKGAVWGNIVWAHSVSTDLINWTPLEPAIFPSKPFDKY 139

Query: 125 GCWSGSATVLPGLKPVILYTGI---DSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDP 184
           GC SGSAT+LPG KPVILYTGI     +N QVQNYAIPANLSDP+L +W+KPDNNP+V  
Sbjct: 140 GCRSGSATILPGNKPVILYTGIVEGPPKNVQVQNYAIPANLSDPYLRKWIKPDNNPLVVA 199

Query: 185 GTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPN 244
               N++AFRDP+TAWL  +GHWK ++GS+  + G+AYLYRSKDF+ WTK + P+HS  N
Sbjct: 200 NNGENATAFRDPTTAWLDKSGHWKMLVGSKRNRRGIAYLYRSKDFIKWTKAKHPIHSQAN 259

Query: 245 TGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPD 304
           TGMWECPDF+PVS  G  GLDTSVTG  VKHVLKVSL++TRYEYYTVG Y    D+Y+PD
Sbjct: 260 TGMWECPDFFPVSLKGLNGLDTSVTGESVKHVLKVSLDLTRYEYYTVGTYLTDKDRYIPD 319

Query: 305 NTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRT 364
           NTS DGW+GLRYDYGNFYASK+F+DP K RR+LWGWANESDS   DV+KGWAGIQ IPRT
Sbjct: 320 NTSVDGWAGLRYDYGNFYASKTFFDPSKNRRILWGWANESDSTAHDVAKGWAGIQLIPRT 379

Query: 365 VWLDPNGKQLLQWPIMELNRLR-RKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSS 424
           +WLDP+GKQL+QWPI EL  LR  K+     Q L KG  VE+KGITAAQ    + FSF S
Sbjct: 380 LWLDPSGKQLMQWPIEELETLRGSKVKFSRKQDLSKGILVEVKGITAAQADVEVTFSFKS 439

Query: 425 LDNAEAFDPSWV--DAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETN 484
           L   E FDP W+  DA+ +CS  GST QGGVGPFGLLTLASE LEE+TPVFFR+FKA+  
Sbjct: 440 LAKREPFDPKWLEYDAEKICSLKGSTVQGGVGPFGLLTLASEKLEEYTPVFFRVFKAQNT 499

Query: 485 HVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSS-KKLPLRSLIDHSVIESFGGGGKTCIT 544
           H VLMCSDA  SSLK+GLY+P+FAGFVD+DL++ KK+ LRSLID+SV+ESFG  GKTCI+
Sbjct: 500 HKVLMCSDATRSSLKEGLYRPSFAGFVDVDLATDKKISLRSLIDNSVVESFGAKGKTCIS 559

Query: 545 SRVYPTKAVFEDAHLHVFNNGTESITVEYLSAWSMR 571
           SRVYPT AV+E+AHL+VFNNG+E+ITVE L AWSM+
Sbjct: 560 SRVYPTLAVYENAHLYVFNNGSETITVENLDAWSMK 586

BLAST of Cp4.1LG12g04200 vs. Swiss-Prot
Match: INV3_DAUCA (Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota GN=INV3 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 7.9e-225
Identity = 376/574 (65.51%), Postives = 446/574 (77.70%), Query Frame = 1

Query: 7   LAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWM 66
           L +L  FSF  L I+     V + H+++PELQS+       ++K +HRTG+HFQP +HW+
Sbjct: 15  LFLLSIFSFIFLNING----VDSTHRVFPELQSIS----AVDVKLVHRTGYHFQPQKHWI 74

Query: 67  NDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGC 126
           NDPNGPM++KG YHLFYQYNP+G+VWGNIVWAHS+SKDLINW +L+PA+ PSKPFD  GC
Sbjct: 75  NDPNGPMFYKGYYHLFYQYNPKGSVWGNIVWAHSVSKDLINWIALEPAIFPSKPFDQYGC 134

Query: 127 WSGSATVLPGLKPVILYTGI---DSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGT 186
           WSGSAT+LPG KPVILYTGI   D +N QVQNYA+PAN SDP L EWVKPDNNP+V   T
Sbjct: 135 WSGSATILPGNKPVILYTGIVSPDPENAQVQNYAVPANYSDPFLREWVKPDNNPLVGVHT 194

Query: 187 EVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTG 246
           E N SAFRDP+TAW    GHWK ++GS  +  G+AYLYRSKDF  W +   P+H+   TG
Sbjct: 195 E-NPSAFRDPTTAWFD-GGHWKMLVGSSRKHRGIAYLYRSKDFKKWKRSPHPIHTKAETG 254

Query: 247 MWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNT 306
           MWECPDFYPVS     GLD S  G  +KHVLKVSL  TRYEYYT+G+Y  + D YVPDNT
Sbjct: 255 MWECPDFYPVSPRSEDGLDNSKMGRGIKHVLKVSLNSTRYEYYTIGRYNRVRDFYVPDNT 314

Query: 307 SADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVW 366
           S DGW+GLRYDYGNFYASK+FYDP+KKRR+LWGWANESDS  DDV KGWAGIQ IPR +W
Sbjct: 315 SVDGWAGLRYDYGNFYASKTFYDPIKKRRILWGWANESDSQIDDVQKGWAGIQLIPRRIW 374

Query: 367 LDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQVII---FSFSSLDN 426
           LDP+G+QL+QWPI E+  LR   + M NQKL  G +VE+ GITAAQ  +   FSF SLD 
Sbjct: 375 LDPSGRQLVQWPIEEVEGLRGSELHMRNQKLDMGVHVEVTGITAAQADVDATFSFKSLDK 434

Query: 427 AEAFDPSWV--DAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHV- 486
           AE+FDP W+  DAQ VC  +GST QGG+GPFGLLTLAS++LEE+TPVFFRIFKAE   + 
Sbjct: 435 AESFDPEWINLDAQDVCDSMGSTIQGGLGPFGLLTLASKDLEEYTPVFFRIFKAEDQKLK 494

Query: 487 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRV 546
           VLMCSDA+ SSL +GLYKP+F GFVD+DLS KK+ LRSLID+SV+ESFG   K  I+SRV
Sbjct: 495 VLMCSDAKRSSLAEGLYKPSFRGFVDVDLSDKKISLRSLIDNSVVESFGAQRKNLISSRV 554

Query: 547 YPTKAVFEDAHLHVFNNGTESITVEYLSAWSMRS 572
           YPT A++ +AHL VFNNGTE ITV+ L AWSM S
Sbjct: 555 YPTLAIYNNAHLFVFNNGTEPITVDNLDAWSMNS 578

BLAST of Cp4.1LG12g04200 vs. Swiss-Prot
Match: INV2_DAUCA (Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota GN=INV2 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 6.7e-224
Identity = 370/561 (65.95%), Postives = 448/561 (79.86%), Query Frame = 1

Query: 20  IDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIY 79
           +D  +  V+A H+++PELQS+   N    ++ +HRTG+HFQP +HW+NDPNGPMY+KG Y
Sbjct: 31  LDVNIRGVEASHQVFPELQSVSAVN----VQLVHRTGYHFQPKKHWINDPNGPMYYKGFY 90

Query: 80  HLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKP 139
           HLFYQYNP+GAVWGNIVWAHSISKDLINW +L+PA+ PSKPFD  GCWSGSATVLPG KP
Sbjct: 91  HLFYQYNPKGAVWGNIVWAHSISKDLINWVALEPAIFPSKPFDKYGCWSGSATVLPGGKP 150

Query: 140 VILYTGIDSQ---NRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTA 199
           VI+YTGI +    N QVQN+A+PAN SDP+L EW+KPDNNPIV   +E NSS+FRDP+TA
Sbjct: 151 VIMYTGIVTPSPVNTQVQNFAVPANYSDPYLREWIKPDNNPIVRARSE-NSSSFRDPTTA 210

Query: 200 WLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHF 259
           W     HWK ++GSR +  G+AYLYRS++FL WTK + PLHS   TGMWEC DFYPV+  
Sbjct: 211 WFD-GVHWKILVGSRRKHRGIAYLYRSRNFLKWTKAKHPLHSKDRTGMWECLDFYPVAPK 270

Query: 260 GRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYG 319
           G  GLDTSVTG  +KHVLKVSL  TRYEYYTVG+Y    D YVPDNTS DGW+GLRYDYG
Sbjct: 271 GMNGLDTSVTGQDIKHVLKVSLYSTRYEYYTVGEYDRDNDIYVPDNTSVDGWAGLRYDYG 330

Query: 320 NFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPI 379
           NFYASK+F+DP K+RR+LWGWANESDS QDDV KGWAGIQ IPR +WLDPNGKQL+QWPI
Sbjct: 331 NFYASKTFFDPDKQRRILWGWANESDSKQDDVQKGWAGIQLIPRKLWLDPNGKQLIQWPI 390

Query: 380 MELNRLRRKIVMMINQKLVKGQNVEIKGITAAQVII---FSFSSLDNAEAFDPSW--VDA 439
            E+  LR + V M +Q L  G+++E+KG+TAAQ  +   FSF SLD AE FDP+W  +DA
Sbjct: 391 EEIQLLRGQNVHMGSQVLNTGEHIEVKGVTAAQADVDATFSFKSLDRAEWFDPNWAKLDA 450

Query: 440 QAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCSDA-RSSSLK 499
             VC  +GST +GG+GPFG LTLASE LEE+TPVFFR+FK +    VLMCSDA RSS+  
Sbjct: 451 LDVCDWMGSTVRGGLGPFGFLTLASEKLEEYTPVFFRVFKTKDKLKVLMCSDAKRSSTTA 510

Query: 500 DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEDAHLH 559
           +GLYKP FAG+VD+DLS KK+ LRSLID+SV+ESFG  G+TCITSRVYP  A++ +AH+ 
Sbjct: 511 EGLYKPPFAGYVDVDLSDKKISLRSLIDNSVVESFGAHGRTCITSRVYPKIAIYNNAHVF 570

Query: 560 VFNNGTESITVEYLSAWSMRS 572
           VFNNGTE+IT++ L AWSM++
Sbjct: 571 VFNNGTEAITIDSLDAWSMKA 585

BLAST of Cp4.1LG12g04200 vs. Swiss-Prot
Match: INV4_ARATH (Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana GN=CWINV4 PE=2 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 1.3e-203
Identity = 333/579 (57.51%), Postives = 428/579 (73.92%), Query Frame = 1

Query: 6   LLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHW 65
           LL +L+  S      +  +  + A H+IY ELQS         +  LHR  FHFQP +HW
Sbjct: 12  LLLVLINLS------NQNIKGIDAFHQIYEELQS----ESVESVNHLHRPSFHFQPPKHW 71

Query: 66  MNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDING 125
           +NDPNGP+Y+KG+YHLFYQYN +GAVWGNI+WAHS+SKDL+NW++L+PALSPSK FDI G
Sbjct: 72  INDPNGPVYYKGLYHLFYQYNTKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGG 131

Query: 126 CWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEV 185
            WSGS T++PG  P+ILYTG++    Q+QNYAIP + SDP+L +W+KPD+NPI  P   +
Sbjct: 132 TWSGSITIVPGKGPIILYTGVNQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTM 191

Query: 186 NSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMW 245
           N SAFRDP+TAW S +GHW+T++GS+ ++ G+AY+YRS+DF +W K + P+HS  +TGMW
Sbjct: 192 NGSAFRDPTTAWFSKDGHWRTVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMW 251

Query: 246 ECPDFYPVSHFG-RFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTS 305
           ECPDF+PVS    R GLD    G   KHVLKVSL++TRYEYYT+GKY    D+Y+PD  +
Sbjct: 252 ECPDFFPVSLTDFRNGLDLDYVGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNT 311

Query: 306 ADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWL 365
            DGW GLR+DYGNFYASK+F+D  K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV L
Sbjct: 312 PDGWEGLRFDYGNFYASKTFFDYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLL 371

Query: 366 DPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSSLDNA 425
           D + KQL+ WP+ E+  LR   V M N  +  GQ +E+KGIT AQ    + F   SL+ A
Sbjct: 372 DSSKKQLVFWPVEEIESLRGNYVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKA 431

Query: 426 EAFDPSWV-DAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNH--VV 485
           E FDPS+      +C+  GS  +GGVGPFGL+TLA+ +LEE+TPVFFR+F     H   V
Sbjct: 432 EIFDPSFTWKPLELCNIKGSNVRGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPKV 491

Query: 486 LMCSDARSSSL--------KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGK 545
           LMCSDAR SSL        KD +YKP+FAGFVD+D++  ++ LRSLIDHSV+ESFG  GK
Sbjct: 492 LMCSDARPSSLKQDTGLLAKDRMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGK 551

Query: 546 TCITSRVYPTKAVFEDAHLHVFNNGTESITVEYLSAWSM 570
           T ITSRVYP KAV E+AHL+VFNNGT+++T+E L+AW+M
Sbjct: 552 TVITSRVYPVKAVKENAHLYVFNNGTQTVTIESLNAWNM 580

BLAST of Cp4.1LG12g04200 vs. Swiss-Prot
Match: INV2_ARATH (Beta-fructofuranosidase, insoluble isoenzyme CWINV2 OS=Arabidopsis thaliana GN=CWINV2 PE=2 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 2.6e-199
Identity = 339/584 (58.05%), Postives = 426/584 (72.95%), Query Frame = 1

Query: 3   FFTLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPH 62
           +  LL +L+  S      +NG   V A HK++ +LQS     ++  +  LHRT +HFQP 
Sbjct: 8   YVLLLIVLINIS------NNG---VDAFHKVFKKLQSKSTSLES--VSPLHRTAYHFQPP 67

Query: 63  RHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFD 122
           RHW+NDPN PM +KG+YHLFYQYNP+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FD
Sbjct: 68  RHWINDPNAPMLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFD 127

Query: 123 INGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG 182
           ING WSGSAT +PG  PVILYTGI     Q+QNYAIP +LSDP+L  W+KPD+NPIV P 
Sbjct: 128 INGTWSGSATHVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPD 187

Query: 183 TEVNSSAFRDPSTAWLS-LNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPN 242
              N SAFRDP+TAW +  +G+W+ ++GS+ +  G+AY+Y+S+DF  W K ++P+HS   
Sbjct: 188 NGENGSAFRDPTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKK 247

Query: 243 TGMWECPDFYPVSHFG-RFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 302
           TGMWECPDF+PVS    + GLD S  G   KHVLKVSL++TRYEYYT+G Y    D+Y P
Sbjct: 248 TGMWECPDFFPVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRP 307

Query: 303 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 362
           D  + DGW GLR+DYGN+YASK+F+D    RR+LWGWANESD+VQDD  KGWAGIQ IPR
Sbjct: 308 DGYTPDGWDGLRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPR 367

Query: 363 TVWLDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSS 422
           T+ LD +GKQL+ WPI E+  LR K V M NQK+  GQ  E++GIT AQV   + F+  +
Sbjct: 368 TILLDSSGKQLVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGN 427

Query: 423 LDNAEAFDPSWVDAQ-AVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFK--AET 482
           L+ AE FD S+      +C+  GS   GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A  
Sbjct: 428 LEKAEKFDESFATKPLELCNLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASN 487

Query: 483 NHVVLMCSDARSSSL--------KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFG 542
              VLMCSDA+ SSL        K+ +YKP+FAGFVD+ L   K+ LRSLIDHSV+ESFG
Sbjct: 488 KPKVLMCSDAKPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFG 547

Query: 543 GGGKTCITSRVYPTKAVFEDAHLHVFNNGTESITVEYLSAWSMR 571
             GKT ITSRVYPTKAV E AHL VFNNG++ +TVE L+AW+M+
Sbjct: 548 AKGKTVITSRVYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNMQ 580

BLAST of Cp4.1LG12g04200 vs. TrEMBL
Match: A0A0A0LAY1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G777580 PE=3 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 3.8e-282
Identity = 462/579 (79.79%), Postives = 511/579 (88.26%), Query Frame = 1

Query: 1   MNFFTLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MN FT L++LLGFSF SLFI+NG+V V ALHKIYPELQS++   D + IK LHRT FHFQ
Sbjct: 1   MNLFTFLSLLLGFSFFSLFINNGVVFVDALHKIYPELQSIQT--DASVIKPLHRTRFHFQ 60

Query: 61  PHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           P R+W+NDPNGPMYFKGIYHLFYQYNP+GAVWGNIVWAHSIS+DLINWK LKPA+ PSKP
Sbjct: 61  PRRNWINDPNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKPAIYPSKP 120

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLPG KPVILYTGID QNRQVQNYAIPANLSDP+LTEW+KPDNNPIVD
Sbjct: 121 FDINGCWSGSATVLPGDKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDNNPIVD 180

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVP 240
           PG  VN+SAFRDP+TAWLS +GHWKT+IGS+ ++ GMAYLYRS+DF+ WTK + PLHS  
Sbjct: 181 PGPGVNASAFRDPTTAWLSKSGHWKTVIGSKRKKRGMAYLYRSRDFVKWTKAKHPLHSAA 240

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPV   G+ GLD SVTGNWVKHV KVSL++TRYEYYTVGKY+P  DKYVP
Sbjct: 241 NTGMWECPDFYPVPLRGKLGLDPSVTGNWVKHVFKVSLDLTRYEYYTVGKYFPKKDKYVP 300

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTS DGWSGLRYDYGNFYASKSFYDP+KKRRVLWGWANESDS QDDVSKGWAGIQ IPR
Sbjct: 301 DNTSVDGWSGLRYDYGNFYASKSFYDPLKKRRVLWGWANESDSAQDDVSKGWAGIQLIPR 360

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSS 420
           TVWLD N +QL QWP+ ELN LR K V++ +QKL+KG  VE+KGITAAQ    ++FSFSS
Sbjct: 361 TVWLDHNQRQLKQWPVKELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVMFSFSS 420

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHV 480
           LD A+ FDP WVDAQA C QLG+ +QGGVGPFGLLTLASENL+EFTPVFFRIFKA   HV
Sbjct: 421 LDKADPFDPRWVDAQAACDQLGN-SQGGVGPFGLLTLASENLDEFTPVFFRIFKAHHKHV 480

Query: 481 VLMCSDARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSR 540
           VLMCSDARSSSLK +GLYKP FAG+VDIDLS KKL LRSLIDHSV+ESFGGGGKTCITSR
Sbjct: 481 VLMCSDARSSSLKEEGLYKPAFAGYVDIDLSRKKLSLRSLIDHSVVESFGGGGKTCITSR 540

Query: 541 VYPTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSAWVN 576
           VYPTK VF+DAHLHVFNNGTE+ITVEYL AWSM+SA +N
Sbjct: 541 VYPTKGVFDDAHLHVFNNGTEAITVEYLRAWSMKSARMN 576

BLAST of Cp4.1LG12g04200 vs. TrEMBL
Match: A0A059DIE8_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02647 PE=3 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 3.6e-240
Identity = 392/577 (67.94%), Postives = 463/577 (80.24%), Query Frame = 1

Query: 4   FTLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHR 63
           F LL +++ F F      +    V+A HK+YPE QSL        +K  HRTG+HFQP +
Sbjct: 15  FYLLLVIVAFDF-----GDENCAVEARHKVYPEYQSLP----AVSVKYAHRTGYHFQPPK 74

Query: 64  HWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDI 123
           HW+NDPNGPM++KG YHLFYQYNP+GAVWGNIVWAHS+SKDL+NW++L PA+ P+KPFDI
Sbjct: 75  HWINDPNGPMHYKGWYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWRALPPAIYPTKPFDI 134

Query: 124 NGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGT 183
           NGCWSGSATVLPG +P+ILYTGID++N QVQNYA+PAN SDP+L EW KPDNNPIVDPG 
Sbjct: 135 NGCWSGSATVLPGNRPIILYTGIDTKNHQVQNYAVPANASDPYLREWTKPDNNPIVDPGP 194

Query: 184 EVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTG 243
            VN+SAFRDP+TAW   +GHWK ++GSR +  GMAYLYRS+DF++WTK + PLHS P TG
Sbjct: 195 GVNASAFRDPTTAWFE-DGHWKILVGSRRKHRGMAYLYRSRDFIHWTKAKHPLHSAPKTG 254

Query: 244 MWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNT 303
           MWECPDF+PVS     GLDTS  G  VKHVLKVSL++TRYEYYT+G Y+P  D+Y+PDNT
Sbjct: 255 MWECPDFFPVSKNNANGLDTSALGQGVKHVLKVSLDLTRYEYYTIGTYHPDKDRYMPDNT 314

Query: 304 SADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVW 363
           SADGWSGLRYDYGNFYASK+F+DP+K RR+LWGWANESD+VQ DV KGWAGIQ IPR +W
Sbjct: 315 SADGWSGLRYDYGNFYASKTFFDPVKNRRLLWGWANESDAVQSDVQKGWAGIQTIPRKIW 374

Query: 364 LDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSSLDN 423
           LD  GKQLLQWP+ EL  LR + V M + KL  G++VEIKGITAAQ    ++FSFSSLD 
Sbjct: 375 LDSYGKQLLQWPVEELETLRGQNVHMSSVKLNNGEHVEIKGITAAQADVDVVFSFSSLDG 434

Query: 424 AEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKA--ETNHVV 483
           AE FD SW++AQ +C   GS   G VGPFGLLTLASE+LEEFTPVFFRIFKA  +  H V
Sbjct: 435 AEQFDSSWLNAQDLCGLKGSGVPGNVGPFGLLTLASEHLEEFTPVFFRIFKADDQNKHKV 494

Query: 484 LMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVY 543
           LMCSDARSSSLK G+YKP+FAGFVD+DLS  K+ LRSLIDHSV+ESFG GGKTCITSRVY
Sbjct: 495 LMCSDARSSSLKSGIYKPSFAGFVDVDLSDGKISLRSLIDHSVVESFGAGGKTCITSRVY 554

Query: 544 PTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSAWVN 576
           PT A+  +A L  FNNGT++IT+E L AWSM    +N
Sbjct: 555 PTLALLGNARLFTFNNGTQTITIETLDAWSMNRPVMN 581

BLAST of Cp4.1LG12g04200 vs. TrEMBL
Match: A0A067FSV9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g008189mg PE=3 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 3.7e-237
Identity = 385/550 (70.00%), Postives = 455/550 (82.73%), Query Frame = 1

Query: 27  VQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 86
           V+A HKIYPE QSL       ++KQLHRT FHFQP +HW+N   GPMY+KGIYHLFYQYN
Sbjct: 25  VEASHKIYPEFQSLA----AVKVKQLHRTAFHFQPPKHWIN---GPMYYKGIYHLFYQYN 84

Query: 87  PEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGI 146
           P+GAVWGNIVWAHS+SKDLINW++L+PAL PSKPFDINGCWSGSATVLPG KP+ILYTG+
Sbjct: 85  PKGAVWGNIVWAHSVSKDLINWEALEPALYPSKPFDINGCWSGSATVLPGNKPIILYTGV 144

Query: 147 DSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLSLNGHWKT 206
           D + RQVQNYA+PAN SDP+L +W+KPDNNP+V PG +VN+SAFRDP+TAW S +GHW+ 
Sbjct: 145 DHKERQVQNYAVPANPSDPYLRKWIKPDNNPVVFPGPDVNASAFRDPTTAWWSNDGHWRM 204

Query: 207 IIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFGRFGLDTSVT 266
           ++GSR +  GMAYLYRS+DF+ WTK + P+HS+ NTGMWECPDFYPVS  G+ GLDTS  
Sbjct: 205 LVGSRRKHRGMAYLYRSRDFMKWTKAKHPIHSLANTGMWECPDFYPVSISGKNGLDTSFA 264

Query: 267 GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYD 326
           G   K VLKVSL++TRY+YYT+G Y    D+YVPD  S DGW GLRYDYGNFYASK+F+D
Sbjct: 265 GGNEKFVLKVSLDLTRYDYYTIGTYNRDKDRYVPDKDSVDGWRGLRYDYGNFYASKTFFD 324

Query: 327 PMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRRKI 386
             K RR+LWGWANESDS  DD++KGWAG+Q IPR VWLD +GKQLLQWPI EL  LR K 
Sbjct: 325 SRKNRRILWGWANESDSTFDDMAKGWAGVQTIPREVWLDGSGKQLLQWPIEELETLRGKN 384

Query: 387 VMMINQKLVKGQNVEIKGITAAQV---IIFSFSSLDNAEAFDPSW--VDAQAVCSQLGST 446
           V + NQ+L KG +VE+KG+TAAQ    + F   SLD AE FDPSW  +DA+ +C ++GS 
Sbjct: 385 VHISNQELKKGHHVEVKGVTAAQADVEVTFKLPSLDKAEKFDPSWKNLDAEHLCGKIGSK 444

Query: 447 AQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCSDARSSSLK-DGLYKPTFAG 506
            QGGVGPFGLLTLAS+NLEEFTPVFFRIFK    H+VLMCSDA SS+LK +GLYKP+FAG
Sbjct: 445 VQGGVGPFGLLTLASKNLEEFTPVFFRIFKDGHKHLVLMCSDASSSTLKTEGLYKPSFAG 504

Query: 507 FVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEDAHLHVFNNGTESIT 566
           FV++DLS KKL LRSLIDHSV+ESFG GGKTCITSRVYPT AVF+DAHL+ FNNGTE++T
Sbjct: 505 FVNVDLSDKKLSLRSLIDHSVVESFGAGGKTCITSRVYPTLAVFDDAHLYAFNNGTETVT 564

Query: 567 VEYLSAWSMR 571
           VE L+AWSM+
Sbjct: 565 VEKLNAWSMK 567

BLAST of Cp4.1LG12g04200 vs. TrEMBL
Match: A0A061EUI0_THECC (Cell wall invertase 2 isoform 1 OS=Theobroma cacao GN=TCM_022641 PE=3 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 4.1e-236
Identity = 380/573 (66.32%), Postives = 464/573 (80.98%), Query Frame = 1

Query: 7   LAILLGFSFCSLF-IDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHW 66
           L IL+ F    +  ++NG   V A HK++P+ QSL    +   ++Q+HRTG+HFQP ++W
Sbjct: 9   LCILITFLCVDIINLNNG--GVDASHKVFPQFQSL----NAVSVQQIHRTGYHFQPPKNW 68

Query: 67  MNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDING 126
           +NDPN PMY+ GIYH FYQYNP+GAVWGNIVWAHS+SKD+INW++L+PAL PS+ FDING
Sbjct: 69  INDPNAPMYYDGIYHFFYQYNPKGAVWGNIVWAHSVSKDMINWQALEPALYPSQHFDING 128

Query: 127 CWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEV 186
           CWSGSATVLP  KP+ILYTG+D ++RQ+QNYA+PANLSDP+L +W+KP NNP+VDP   V
Sbjct: 129 CWSGSATVLPSNKPIILYTGLDPKDRQIQNYAVPANLSDPYLRKWIKPGNNPVVDPEQGV 188

Query: 187 NSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMW 246
           N+SAFRDP+TAW  LNGHW+ ++GS+    GMAYLYRS+DF+ WTK + PLHS P+TGMW
Sbjct: 189 NASAFRDPTTAWW-LNGHWRMLVGSKRNHRGMAYLYRSRDFIYWTKAKHPLHSAPHTGMW 248

Query: 247 ECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSA 306
           ECPDFYPV   G+ GLD SV G  VKHVLKVSL++TRY+YYTVG Y    D+YVPDNTS 
Sbjct: 249 ECPDFYPVLLSGKKGLDPSVLGKHVKHVLKVSLDVTRYDYYTVGTYLSDKDRYVPDNTSV 308

Query: 307 DGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLD 366
           DGW+GLRYDYGNFYASK+F+DP KKRR+LWGW NESD+ +DDV KGWAG+QAIPR VWLD
Sbjct: 309 DGWAGLRYDYGNFYASKTFFDPAKKRRILWGWVNESDTTEDDVKKGWAGLQAIPRIVWLD 368

Query: 367 PNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSSLDNAE 426
           PN +QL+QWPI EL  LR K V M +Q+L KG+++E+KGITAAQ    + F   +LD AE
Sbjct: 369 PNQRQLMQWPIEELETLRGKNVKMSHQQLKKGEHIEVKGITAAQADVDVTFHIPNLDKAE 428

Query: 427 AFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCS 486
            FD SW DAQ +C + GS  +G VGPFGLLTLAS+NLEE+TPV FR+F+    HVVL+CS
Sbjct: 429 PFDHSWTDAQELCVKKGSKVKGEVGPFGLLTLASKNLEEYTPVSFRVFQTSKKHVVLLCS 488

Query: 487 DARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKA 546
           DARSSSLKDGLYKP+FAGFVD+DL+ KKL LRSLIDHSV+ESFG GGKTCITSRVYPT A
Sbjct: 489 DARSSSLKDGLYKPSFAGFVDVDLADKKLSLRSLIDHSVVESFGAGGKTCITSRVYPTLA 548

Query: 547 VFEDAHLHVFNNGTESITVEYLSAWSMRSAWVN 576
           V ++AHL+ FN+GTE I VE L+AWSM+   +N
Sbjct: 549 VLDNAHLYAFNHGTEPINVENLNAWSMKKPRMN 574

BLAST of Cp4.1LG12g04200 vs. TrEMBL
Match: A0A0N6XFM9_RHUCH (Cell wall invertase OS=Rhus chinensis PE=2 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 5.3e-236
Identity = 386/574 (67.25%), Postives = 461/574 (80.31%), Query Frame = 1

Query: 7   LAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWM 66
           LAI L  +   L  ++G   ++A H +YPE QSL        +KQ HRTGFHFQP +HW+
Sbjct: 7   LAISLFLASLFLITNHG---IEASHVVYPEFQSLA----AVTVKQTHRTGFHFQPPKHWI 66

Query: 67  NDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGC 126
           NDPNGPMY+ GIYH FYQYNP+GAVWGNIVWAHS+S+D+INW++L+PA+ PSKPFDI GC
Sbjct: 67  NDPNGPMYYNGIYHFFYQYNPKGAVWGNIVWAHSVSRDMINWQALEPAIYPSKPFDIKGC 126

Query: 127 WSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVN 186
           WSGS T+LPG KP+ILYTGIDS+ RQ+Q+YA+P NLSDP+L EWVKPDNNP+V P   VN
Sbjct: 127 WSGSVTILPGNKPIILYTGIDSEERQLQDYAVPKNLSDPYLREWVKPDNNPLVFPHG-VN 186

Query: 187 SSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWE 246
           +SAFRDP+TAW   +GHW+ ++GSR +Q G+AYLYRSKDF+ WTK + P+HS   TGMWE
Sbjct: 187 ASAFRDPTTAWWEDDGHWRMLVGSRRKQRGIAYLYRSKDFMKWTKAKHPIHSKEKTGMWE 246

Query: 247 CPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSAD 306
           CPDF+PVS  G  GLDTS  G  +K+V K+SL+MTRY+YYTVG Y+   D+YVPDNTS D
Sbjct: 247 CPDFFPVSLSGTNGLDTSYRGPNLKYVFKISLDMTRYDYYTVGSYFRDKDRYVPDNTSVD 306

Query: 307 GWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDP 366
           GW GLRYDYGNFYASK+F+D  K RR+LWGWANESDS  DDV KGWAG+QAIPR++WLDP
Sbjct: 307 GWDGLRYDYGNFYASKTFFDAGKNRRILWGWANESDSKDDDVDKGWAGVQAIPRSLWLDP 366

Query: 367 NGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEI-KGITAAQV---IIFSFSSLDNAE 426
           +GKQ+LQWPI EL  LR K   + N+ L  G+NVE+ +GITAAQ    + FSF SLD AE
Sbjct: 367 SGKQVLQWPIQELETLRGKNAEISNKVLQSGENVEVGQGITAAQADVEVTFSFPSLDKAE 426

Query: 427 AFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCS 486
            FDPSWV+A+ +C Q GST +GGVGPFGLLTLASE LEE+TPVFFRIFKA   HVVLMCS
Sbjct: 427 PFDPSWVNAEKLCGQKGSTVKGGVGPFGLLTLASEKLEEYTPVFFRIFKAPNKHVVLMCS 486

Query: 487 DARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTK 546
           DA SSSLK  GLYKP+FAGFVD+DL+ KKL LRSLIDHSV+ESFG GGKTCITSRVYPT 
Sbjct: 487 DATSSSLKTTGLYKPSFAGFVDVDLADKKLSLRSLIDHSVVESFGAGGKTCITSRVYPTL 546

Query: 547 AVFEDAHLHVFNNGTESITVEYLSAWSMRSAWVN 576
           AVF +AHL  FNNGTE++T++ L+AWSM    +N
Sbjct: 547 AVFSNAHLFAFNNGTETVTIDKLNAWSMNKPQMN 572

BLAST of Cp4.1LG12g04200 vs. TAIR10
Match: AT2G36190.1 (AT2G36190.1 cell wall invertase 4)

HSP 1 Score: 710.7 bits (1833), Expect = 7.4e-205
Identity = 333/579 (57.51%), Postives = 428/579 (73.92%), Query Frame = 1

Query: 6   LLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHW 65
           LL +L+  S      +  +  + A H+IY ELQS         +  LHR  FHFQP +HW
Sbjct: 12  LLLVLINLS------NQNIKGIDAFHQIYEELQS----ESVESVNHLHRPSFHFQPPKHW 71

Query: 66  MNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDING 125
           +NDPNGP+Y+KG+YHLFYQYN +GAVWGNI+WAHS+SKDL+NW++L+PALSPSK FDI G
Sbjct: 72  INDPNGPVYYKGLYHLFYQYNTKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGG 131

Query: 126 CWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEV 185
            WSGS T++PG  P+ILYTG++    Q+QNYAIP + SDP+L +W+KPD+NPI  P   +
Sbjct: 132 TWSGSITIVPGKGPIILYTGVNQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTM 191

Query: 186 NSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMW 245
           N SAFRDP+TAW S +GHW+T++GS+ ++ G+AY+YRS+DF +W K + P+HS  +TGMW
Sbjct: 192 NGSAFRDPTTAWFSKDGHWRTVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMW 251

Query: 246 ECPDFYPVSHFG-RFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTS 305
           ECPDF+PVS    R GLD    G   KHVLKVSL++TRYEYYT+GKY    D+Y+PD  +
Sbjct: 252 ECPDFFPVSLTDFRNGLDLDYVGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNT 311

Query: 306 ADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWL 365
            DGW GLR+DYGNFYASK+F+D  K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV L
Sbjct: 312 PDGWEGLRFDYGNFYASKTFFDYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLL 371

Query: 366 DPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSSLDNA 425
           D + KQL+ WP+ E+  LR   V M N  +  GQ +E+KGIT AQ    + F   SL+ A
Sbjct: 372 DSSKKQLVFWPVEEIESLRGNYVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKA 431

Query: 426 EAFDPSWV-DAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNH--VV 485
           E FDPS+      +C+  GS  +GGVGPFGL+TLA+ +LEE+TPVFFR+F     H   V
Sbjct: 432 EIFDPSFTWKPLELCNIKGSNVRGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPKV 491

Query: 486 LMCSDARSSSL--------KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGK 545
           LMCSDAR SSL        KD +YKP+FAGFVD+D++  ++ LRSLIDHSV+ESFG  GK
Sbjct: 492 LMCSDARPSSLKQDTGLLAKDRMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGK 551

Query: 546 TCITSRVYPTKAVFEDAHLHVFNNGTESITVEYLSAWSM 570
           T ITSRVYP KAV E+AHL+VFNNGT+++T+E L+AW+M
Sbjct: 552 TVITSRVYPVKAVKENAHLYVFNNGTQTVTIESLNAWNM 580

BLAST of Cp4.1LG12g04200 vs. TAIR10
Match: AT3G52600.1 (AT3G52600.1 cell wall invertase 2)

HSP 1 Score: 696.4 bits (1796), Expect = 1.4e-200
Identity = 339/584 (58.05%), Postives = 426/584 (72.95%), Query Frame = 1

Query: 3   FFTLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPH 62
           +  LL +L+  S      +NG   V A HK++ +LQS     ++  +  LHRT +HFQP 
Sbjct: 8   YVLLLIVLINIS------NNG---VDAFHKVFKKLQSKSTSLES--VSPLHRTAYHFQPP 67

Query: 63  RHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFD 122
           RHW+NDPN PM +KG+YHLFYQYNP+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FD
Sbjct: 68  RHWINDPNAPMLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFD 127

Query: 123 INGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG 182
           ING WSGSAT +PG  PVILYTGI     Q+QNYAIP +LSDP+L  W+KPD+NPIV P 
Sbjct: 128 INGTWSGSATHVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPD 187

Query: 183 TEVNSSAFRDPSTAWLS-LNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPN 242
              N SAFRDP+TAW +  +G+W+ ++GS+ +  G+AY+Y+S+DF  W K ++P+HS   
Sbjct: 188 NGENGSAFRDPTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKK 247

Query: 243 TGMWECPDFYPVSHFG-RFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 302
           TGMWECPDF+PVS    + GLD S  G   KHVLKVSL++TRYEYYT+G Y    D+Y P
Sbjct: 248 TGMWECPDFFPVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRP 307

Query: 303 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 362
           D  + DGW GLR+DYGN+YASK+F+D    RR+LWGWANESD+VQDD  KGWAGIQ IPR
Sbjct: 308 DGYTPDGWDGLRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPR 367

Query: 363 TVWLDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSS 422
           T+ LD +GKQL+ WPI E+  LR K V M NQK+  GQ  E++GIT AQV   + F+  +
Sbjct: 368 TILLDSSGKQLVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGN 427

Query: 423 LDNAEAFDPSWVDAQ-AVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFK--AET 482
           L+ AE FD S+      +C+  GS   GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A  
Sbjct: 428 LEKAEKFDESFATKPLELCNLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASN 487

Query: 483 NHVVLMCSDARSSSL--------KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFG 542
              VLMCSDA+ SSL        K+ +YKP+FAGFVD+ L   K+ LRSLIDHSV+ESFG
Sbjct: 488 KPKVLMCSDAKPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFG 547

Query: 543 GGGKTCITSRVYPTKAVFEDAHLHVFNNGTESITVEYLSAWSMR 571
             GKT ITSRVYPTKAV E AHL VFNNG++ +TVE L+AW+M+
Sbjct: 548 AKGKTVITSRVYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNMQ 580

BLAST of Cp4.1LG12g04200 vs. TAIR10
Match: AT3G13790.1 (AT3G13790.1 Glycosyl hydrolases family 32 protein)

HSP 1 Score: 656.4 bits (1692), Expect = 1.7e-188
Identity = 314/571 (54.99%), Postives = 407/571 (71.28%), Query Frame = 1

Query: 17  SLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNDPNGPMYFK 76
           +L I N +V ++A H +Y  L    N   +  + Q +RTGFHFQP ++WMNDPNGPM +K
Sbjct: 16  TLLIGNYVVNLEASHHVYKRLTQSTN-TKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIYK 75

Query: 77  GIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPG 136
           GIYHLFYQ+NP+GAVWGNIVWAHS S DLINW    PA+ PS PFDINGCWSGSAT+LP 
Sbjct: 76  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 135

Query: 137 LKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG--TEVNSSAFRDPS 196
            KPVILYTGID +N+QVQN A P NLSDP+L EW K   NP++ P     +N+S+FRDP+
Sbjct: 136 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 195

Query: 197 TAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYPVS 256
           TAWL  +  W+ IIGS+  + G+A  Y SKDFL W K  +PLH    +GMWECPDF+PV+
Sbjct: 196 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 255

Query: 257 HFGRFGLDTSVTG---NWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGL 316
            FG  G++TS  G     +KHVLK+SL+ T+++YYT+G Y  + DK+VPDN      +  
Sbjct: 256 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 315

Query: 317 RYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQL 376
           RYDYG +YASK+F+D  K RR+LWGW NES SV+DDV KGW+GIQ IPR +WLD +GKQL
Sbjct: 316 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 375

Query: 377 LQWPIMELNRLRRKIVMMINQKLVK-GQNVEIKGITAAQV---IIFSFSSLDNAEAFDPS 436
           +QWP+ E+ RLR K V  +  K++K G  +E+ G+TAAQ    ++F    L+ A+  +PS
Sbjct: 376 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 435

Query: 437 WVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETN---HVVLMCSDA 496
           W D Q +CS++  + + G+GPFGL+ LAS+NLEE+T V+FRIFKA  N   +VVLMCSD 
Sbjct: 436 WTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQ 495

Query: 497 RSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVF 556
             SSLK+   K T+  FVDI+   + L LR+LIDHSV+ESFGG G+ CITSRVYP  A+ 
Sbjct: 496 SRSSLKEDNDKTTYGAFVDIN-PHQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIG 555

Query: 557 EDAHLHVFNNGTESITVEYLSAWSMRSAWVN 576
           + +HL  FN G +S+ V  L+AWSM SA ++
Sbjct: 556 KSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of Cp4.1LG12g04200 vs. TAIR10
Match: AT1G55120.1 (AT1G55120.1 beta-fructofuranosidase 5)

HSP 1 Score: 623.6 bits (1607), Expect = 1.2e-178
Identity = 295/542 (54.43%), Postives = 381/542 (70.30%), Query Frame = 1

Query: 48  EIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWG-NIVWAHSISKDLI 107
           ++ Q +RTG+HFQP ++WMNDPNGPM +KGIYHLFYQYNP GAVW   IVW HS S DL+
Sbjct: 33  DLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLV 92

Query: 108 NWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPH 167
           NW S  PA +PS+P DINGCWSGS T+LP  KPVILYTGID    QVQN A+P N+SDP+
Sbjct: 93  NWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPY 152

Query: 168 LTEWVKPDNNPIVDPGTE--VNSSAFRDPSTAWLSLNGHWKTIIGSRSE-QTGMAYLYRS 227
           L EW KP  NP++       +N   FRDP+TAWL  +G W+ I+GS ++ + G+A LY+S
Sbjct: 153 LREWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKS 212

Query: 228 KDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFGRFGLDTSVTG-NWVKHVLKVSLEMTR 287
           +DF NWT+  +PLH    TGMWECPDF+PVS  G  G++TS  G N +KHVLKVSL  T 
Sbjct: 213 RDFFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETL 272

Query: 288 YEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESD 347
           ++YYT+G Y    D YVPD       S  R DYG +YASK+FYD +KKRR+LWGW NES 
Sbjct: 273 HDYYTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESS 332

Query: 348 SVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEI 407
             +DD+ KGW+G+Q+ PR +WLD +GK+LLQWPI E+  LR + V    + L  G  +++
Sbjct: 333 PAKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQV 392

Query: 408 KGITAAQV---IIFSFSSLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENL 467
            G+TAAQ    + F    L+ A+  +PSW D Q +CSQ   +   G+GPFGL+ LAS ++
Sbjct: 393 HGVTAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDM 452

Query: 468 EEFTPVFFRIFKA------ETNHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPL 527
           EE+T V+FRIFK+      +T +VVLMCSD   SSL D   K TF  FV ID S + + L
Sbjct: 453 EEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISL 512

Query: 528 RSLIDHSVIESFGGGGKTCITSRVYPTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSAW 576
           R+LIDHS++ES+GGGG+TCITSRVYP  A+ E+A+L VFN GT+S+ +  LSAWS++SA 
Sbjct: 513 RTLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQ 572

BLAST of Cp4.1LG12g04200 vs. TAIR10
Match: AT3G13784.1 (AT3G13784.1 cell wall invertase 5)

HSP 1 Score: 576.2 bits (1484), Expect = 2.2e-164
Identity = 293/574 (51.05%), Postives = 378/574 (65.85%), Query Frame = 1

Query: 13  FSFCSLFI-DNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNDPNG 72
           F   SLF+ D+ +VV+ AL  +   +++           Q +RTG+HFQP ++WMN   G
Sbjct: 12  FLLVSLFLTDDAVVVLDALDNVPNNIKN-----------QPYRTGYHFQPPKNWMN---G 71

Query: 73  PMYFKGIYHLFYQYNPEGAVW--GNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSG 132
           PM +KGIYHLFYQ+N  GAV      VW H+ S DLINW +L PA+ PS+P DINGCWSG
Sbjct: 72  PMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINWITLSPAIRPSRPSDINGCWSG 131

Query: 133 SATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG--TEVNS 192
           S T+LP  KPVILYTG D  NRQVQN   P NL+DP+L  W K   NP+V P     +NS
Sbjct: 132 SVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLRHWTKSPENPLVTPSPVNHINS 191

Query: 193 SAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWEC 252
           SAFRDP+TAW   +G W+   GS+  + G+A L+ SKDF+ W +  +PLH    TGMWEC
Sbjct: 192 SAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDFVIWKQSPKPLHYHDGTGMWEC 251

Query: 253 PDFYPVSHFGRFGLDTSVT-GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSAD 312
           PDF+PV+     GLDTS + G  VKHVLKVSL  T  +YYT+G Y  + D YVPD     
Sbjct: 252 PDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFNDYYTIGTYDEVRDVYVPDKGFVQ 311

Query: 313 GWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDP 372
             +  RYDYG FYASK+FYD + +RR+LWGW NES   +D++ KGWAG+QAIPR VWLD 
Sbjct: 312 DETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPEKDNIKKGWAGLQAIPRKVWLDE 371

Query: 373 NGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFS--SLDNA 432
           +GK+L+QWP+ E+ RLR   V   N+ L  G  +E+ G+TA Q    + F  S   L+ A
Sbjct: 372 SGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHGVTAPQADVEVFFKVSGFDLEKA 431

Query: 433 EAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETN---HVV 492
           +  +P W D Q +CSQ    A  G+GPFGL+ LAS+N+EE+T V  RIF+A  N   HVV
Sbjct: 432 DVIEPGWTDPQLICSQ--RNASSGLGPFGLMVLASKNMEEYTSVNIRIFRAGENSKEHVV 491

Query: 493 LMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVY 552
           +MCSD  +SSL+ G  K T+  F+DI    + + LR+LID S++ESFGG GKTCITSRVY
Sbjct: 492 VMCSDQSTSSLEKGNDKTTYGAFLDIS-PYQPISLRTLIDKSIVESFGGKGKTCITSRVY 551

Query: 553 PTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSA 573
           P  A+ E  HL  FN G++++ V  LSAWSM+S+
Sbjct: 552 PKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568

BLAST of Cp4.1LG12g04200 vs. NCBI nr
Match: gi|449462946|ref|XP_004149196.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucumis sativus])

HSP 1 Score: 978.4 bits (2528), Expect = 5.4e-282
Identity = 462/579 (79.79%), Postives = 511/579 (88.26%), Query Frame = 1

Query: 1   MNFFTLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MN FT L++LLGFSF SLFI+NG+V V ALHKIYPELQS++   D + IK LHRT FHFQ
Sbjct: 1   MNLFTFLSLLLGFSFFSLFINNGVVFVDALHKIYPELQSIQT--DASVIKPLHRTRFHFQ 60

Query: 61  PHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           P R+W+NDPNGPMYFKGIYHLFYQYNP+GAVWGNIVWAHSIS+DLINWK LKPA+ PSKP
Sbjct: 61  PRRNWINDPNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKPAIYPSKP 120

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLPG KPVILYTGID QNRQVQNYAIPANLSDP+LTEW+KPDNNPIVD
Sbjct: 121 FDINGCWSGSATVLPGDKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDNNPIVD 180

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVP 240
           PG  VN+SAFRDP+TAWLS +GHWKT+IGS+ ++ GMAYLYRS+DF+ WTK + PLHS  
Sbjct: 181 PGPGVNASAFRDPTTAWLSKSGHWKTVIGSKRKKRGMAYLYRSRDFVKWTKAKHPLHSAA 240

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPV   G+ GLD SVTGNWVKHV KVSL++TRYEYYTVGKY+P  DKYVP
Sbjct: 241 NTGMWECPDFYPVPLRGKLGLDPSVTGNWVKHVFKVSLDLTRYEYYTVGKYFPKKDKYVP 300

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTS DGWSGLRYDYGNFYASKSFYDP+KKRRVLWGWANESDS QDDVSKGWAGIQ IPR
Sbjct: 301 DNTSVDGWSGLRYDYGNFYASKSFYDPLKKRRVLWGWANESDSAQDDVSKGWAGIQLIPR 360

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSS 420
           TVWLD N +QL QWP+ ELN LR K V++ +QKL+KG  VE+KGITAAQ    ++FSFSS
Sbjct: 361 TVWLDHNQRQLKQWPVKELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVMFSFSS 420

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHV 480
           LD A+ FDP WVDAQA C QLG+ +QGGVGPFGLLTLASENL+EFTPVFFRIFKA   HV
Sbjct: 421 LDKADPFDPRWVDAQAACDQLGN-SQGGVGPFGLLTLASENLDEFTPVFFRIFKAHHKHV 480

Query: 481 VLMCSDARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSR 540
           VLMCSDARSSSLK +GLYKP FAG+VDIDLS KKL LRSLIDHSV+ESFGGGGKTCITSR
Sbjct: 481 VLMCSDARSSSLKEEGLYKPAFAGYVDIDLSRKKLSLRSLIDHSVVESFGGGGKTCITSR 540

Query: 541 VYPTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSAWVN 576
           VYPTK VF+DAHLHVFNNGTE+ITVEYL AWSM+SA +N
Sbjct: 541 VYPTKGVFDDAHLHVFNNGTEAITVEYLRAWSMKSARMN 576

BLAST of Cp4.1LG12g04200 vs. NCBI nr
Match: gi|659084391|ref|XP_008442862.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucumis melo])

HSP 1 Score: 976.9 bits (2524), Expect = 1.6e-281
Identity = 464/575 (80.70%), Postives = 507/575 (88.17%), Query Frame = 1

Query: 5   TLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRH 64
           T L++LLGFSF SLFI+NG   V+ALHKIYPELQS++   D + +K LHRT FHFQP R+
Sbjct: 6   TFLSLLLGFSFFSLFINNG---VEALHKIYPELQSIQT--DASVVKPLHRTRFHFQPRRN 65

Query: 65  WMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDIN 124
           W+NDPNGPMYF GIYHLFYQYNP+GAVWGNIVWAHSIS+DLINWK LK A+ PSKPFDIN
Sbjct: 66  WINDPNGPMYFNGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKAAIYPSKPFDIN 125

Query: 125 GCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTE 184
           GCWSGSATVLPG KPVILYTGID QNRQVQNYAIPANLSDP+LTEW+KPD+NPIVDPG  
Sbjct: 126 GCWSGSATVLPGNKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDHNPIVDPGPG 185

Query: 185 VNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGM 244
           VN+SAFRDP+TAWLS +GHWKTIIGS+ ++ GMAYLYRS+DF+ WTK + PLHS  NTGM
Sbjct: 186 VNASAFRDPTTAWLSKSGHWKTIIGSKRKRRGMAYLYRSRDFVKWTKAKHPLHSAANTGM 245

Query: 245 WECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTS 304
           WECPDFYPV   GR GLD SVTGNWVKHVLKVSL++TRYEYYTVGKY+P  DKYVPDNTS
Sbjct: 246 WECPDFYPVPLRGRLGLDPSVTGNWVKHVLKVSLDLTRYEYYTVGKYFPKKDKYVPDNTS 305

Query: 305 ADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWL 364
            DGWSGLRYDYGNFYASKSFYDP++KRRVLWGWANESDS QDDVSKGWAGIQ IPRTVWL
Sbjct: 306 VDGWSGLRYDYGNFYASKSFYDPLRKRRVLWGWANESDSAQDDVSKGWAGIQLIPRTVWL 365

Query: 365 DPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSSLDNA 424
           D N KQL+QWP+ ELN LR K V++ +QKL+KG  VE+KGITAAQ    +IFSFSSLD A
Sbjct: 366 DHNQKQLIQWPVEELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVIFSFSSLDKA 425

Query: 425 EAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMC 484
           E FDP WVDAQA C QLG  +QGGVGPFGLLTLASENLEEFTPVFFRIFKA   HVVLMC
Sbjct: 426 EPFDPRWVDAQAACDQLGLKSQGGVGPFGLLTLASENLEEFTPVFFRIFKAHHKHVVLMC 485

Query: 485 SDARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPT 544
           SDARSSSLK DGLYKP FAG+VDIDLSSKKL LRSLIDHSV+ESFGGGGKTCITSRVYPT
Sbjct: 486 SDARSSSLKEDGLYKPAFAGYVDIDLSSKKLSLRSLIDHSVVESFGGGGKTCITSRVYPT 545

Query: 545 KAVFEDAHLHVFNNGTESITVEYLSAWSMRSAWVN 576
           K VF DAHLHVFNNGTE+ITVEYLSAWSM+SA VN
Sbjct: 546 KGVFNDAHLHVFNNGTEAITVEYLSAWSMKSARVN 575

BLAST of Cp4.1LG12g04200 vs. NCBI nr
Match: gi|568856346|ref|XP_006481745.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like [Citrus sinensis])

HSP 1 Score: 842.0 bits (2174), Expect = 6.0e-241
Identity = 388/550 (70.55%), Postives = 458/550 (83.27%), Query Frame = 1

Query: 27  VQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 86
           V+A HKIYPE QSL       ++KQLHRT FHFQP +HW+NDPNGPMY+KGIYHLFYQYN
Sbjct: 25  VEASHKIYPEFQSLA----AVKVKQLHRTAFHFQPPKHWINDPNGPMYYKGIYHLFYQYN 84

Query: 87  PEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGI 146
           P+GAVWGNIVWAHS+SKDLINW++L+PAL PSKPFDINGCWSGSATVLPG KP+ILYTG+
Sbjct: 85  PKGAVWGNIVWAHSVSKDLINWEALEPALYPSKPFDINGCWSGSATVLPGNKPIILYTGV 144

Query: 147 DSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLSLNGHWKT 206
           D + RQVQNYA+PAN SDP+L +W+KPDNNP+V PG +VN+SAFRDP+TAW S +GHW+ 
Sbjct: 145 DHKERQVQNYAVPANPSDPYLRKWIKPDNNPVVFPGPDVNASAFRDPTTAWWSNDGHWRM 204

Query: 207 IIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFGRFGLDTSVT 266
           ++GSR +  GMAYLYRS+DF+ WTK + P+HS+ NTGMWECPDFYPVS  G+ GLDTS  
Sbjct: 205 LVGSRRKHRGMAYLYRSRDFMKWTKAKHPIHSLANTGMWECPDFYPVSISGKNGLDTSFA 264

Query: 267 GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYD 326
           G   K VLKVSL++TRY+YYT+G Y    D+YVPD  S DGW GLRYDYGNFYASK+F+D
Sbjct: 265 GGNEKFVLKVSLDLTRYDYYTIGTYNRDKDRYVPDKDSVDGWRGLRYDYGNFYASKTFFD 324

Query: 327 PMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRRKI 386
             K RR+LWGWANESDS  DD++KGWAG+Q IPR VWLD +GKQLLQWPI EL  LR K 
Sbjct: 325 SRKNRRILWGWANESDSTFDDMAKGWAGVQTIPREVWLDGSGKQLLQWPIEELETLRGKN 384

Query: 387 VMMINQKLVKGQNVEIKGITAAQV---IIFSFSSLDNAEAFDPSW--VDAQAVCSQLGST 446
           V + NQ+L KG +VE+KG+TAAQ    + F   SLD AE FDPSW  +DA+ +C ++GS 
Sbjct: 385 VHISNQELKKGHHVEVKGVTAAQADVEVTFKLPSLDKAEKFDPSWKNLDAEHLCGKIGSK 444

Query: 447 AQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCSDARSSSLK-DGLYKPTFAG 506
            QGGVGPFGLLTLAS+NLEEFTPVFFRIFK    H+VLMCSDA SS+LK +GLYKP+FAG
Sbjct: 445 VQGGVGPFGLLTLASKNLEEFTPVFFRIFKDGHKHLVLMCSDASSSTLKTEGLYKPSFAG 504

Query: 507 FVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEDAHLHVFNNGTESIT 566
           FV++DLS KKL LRSLIDHSV+ESFG GGKTCITSRVYPT AVF+DAHL+ FNNGTE++T
Sbjct: 505 FVNVDLSDKKLSLRSLIDHSVVESFGAGGKTCITSRVYPTLAVFDDAHLYAFNNGTETVT 564

Query: 567 VEYLSAWSMR 571
           VE L+AWSM+
Sbjct: 565 VEKLNAWSMK 570

BLAST of Cp4.1LG12g04200 vs. NCBI nr
Match: gi|657989615|ref|XP_008387018.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like isoform X1 [Malus domestica])

HSP 1 Score: 842.0 bits (2174), Expect = 6.0e-241
Identity = 390/569 (68.54%), Postives = 461/569 (81.02%), Query Frame = 1

Query: 13  FSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNDPNGP 72
           F  C +F  N +  V+ALH +YPE QSL       ++KQ+HRT FHFQP R+W+NDPNGP
Sbjct: 15  FLACCVFAINNITGVEALHNVYPEFQSLA----AVKVKQIHRTAFHFQPPRNWINDPNGP 74

Query: 73  MYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSAT 132
           MYF G+YHLFYQYNPEGAVWGNIVWAHS+SKDLINW++L+PA+ PSKPFD+NGCWSGSAT
Sbjct: 75  MYFGGLYHLFYQYNPEGAVWGNIVWAHSVSKDLINWEALEPAIYPSKPFDVNGCWSGSAT 134

Query: 133 VLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRD 192
            LPG KP++LYTG+DSQNRQVQNYAIP N SDP+L +W+KPDNNP+V P T +N++AFRD
Sbjct: 135 XLPGNKPIMLYTGVDSQNRQVQNYAIPKNASDPYLRKWIKPDNNPLVIPDTGINATAFRD 194

Query: 193 PSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTGMWECPDFYP 252
           P+TAW   NGHWK ++G + +  GMA+LYRSKDF++W K   PLHS P TGMWECPDFYP
Sbjct: 195 PTTAWWG-NGHWKMLVGGKRKHRGMAHLYRSKDFVHWVKAHHPLHSAPQTGMWECPDFYP 254

Query: 253 VSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLR 312
           V   G+ GLDTS  G  VKHVLKVSL+ TRYEYYTVGKY+P  D YVPD    DGWSGLR
Sbjct: 255 VPLVGKLGLDTSKVGGDVKHVLKVSLDETRYEYYTVGKYFPEKDTYVPDKELVDGWSGLR 314

Query: 313 YDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLL 372
           YDYGNFYASK+F+DP KKRR+LWGWANESD+  +DV+KGWAGIQ IPR VWL P+GKQLL
Sbjct: 315 YDYGNFYASKTFFDPAKKRRILWGWANESDTASNDVAKGWAGIQTIPRDVWLSPDGKQLL 374

Query: 373 QWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSSLDNAEAFDPSW- 432
           QWP++EL  LR K V + NQ L +G +VE+KG+TAAQ    +IFS  SL  AE F+PSW 
Sbjct: 375 QWPVVELETLRGKKVGINNQILQQGSHVEVKGLTAAQADVEVIFSLPSLAKAEEFNPSWS 434

Query: 433 -VDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHVVLMCSDARSS 492
            +DAQ +C + GS  QGG+GPFGLLTLASENLEEFTPVFFR+FKA+  HVVLMCS A SS
Sbjct: 435 NLDAQTLCGKKGSKIQGGIGPFGLLTLASENLEEFTPVFFRVFKAKGRHVVLMCSXATSS 494

Query: 493 SLKDGLYKPTFAGFVDIDLS-SKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFED 552
           SL+D LY+P+FAGFVD+DLS SKKL LRSLIDHSV+ES+G GGKTCITSRVYPT AV   
Sbjct: 495 SLQDNLYRPSFAGFVDVDLSHSKKLSLRSLIDHSVVESYGAGGKTCITSRVYPTLAVNNG 554

Query: 553 AHLHVFNNGTESITVEYLSAWSMRSAWVN 576
           AHL+VFNNGT+ +T E L+AWSM +  +N
Sbjct: 555 AHLYVFNNGTQPVTAESLNAWSMNAPTMN 578

BLAST of Cp4.1LG12g04200 vs. NCBI nr
Match: gi|702249497|ref|XP_010059629.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 3-like [Eucalyptus grandis])

HSP 1 Score: 839.0 bits (2166), Expect = 5.1e-240
Identity = 392/577 (67.94%), Postives = 463/577 (80.24%), Query Frame = 1

Query: 4   FTLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHR 63
           F LL +++ F F      +    V+A HK+YPE QSL        +K  HRTG+HFQP +
Sbjct: 15  FYLLLVIVAFDF-----GDENCAVEARHKVYPEYQSLP----AVSVKYAHRTGYHFQPPK 74

Query: 64  HWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDI 123
           HW+NDPNGPM++KG YHLFYQYNP+GAVWGNIVWAHS+SKDL+NW++L PA+ P+KPFDI
Sbjct: 75  HWINDPNGPMHYKGWYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWRALPPAIYPTKPFDI 134

Query: 124 NGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGT 183
           NGCWSGSATVLPG +P+ILYTGID++N QVQNYA+PAN SDP+L EW KPDNNPIVDPG 
Sbjct: 135 NGCWSGSATVLPGNRPIILYTGIDTKNHQVQNYAVPANASDPYLREWTKPDNNPIVDPGP 194

Query: 184 EVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVPNTG 243
            VN+SAFRDP+TAW   +GHWK ++GSR +  GMAYLYRS+DF++WTK + PLHS P TG
Sbjct: 195 GVNASAFRDPTTAWFE-DGHWKILVGSRRKHRGMAYLYRSRDFIHWTKAKHPLHSAPKTG 254

Query: 244 MWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNT 303
           MWECPDF+PVS     GLDTS  G  VKHVLKVSL++TRYEYYT+G Y+P  D+Y+PDNT
Sbjct: 255 MWECPDFFPVSKNNANGLDTSALGQGVKHVLKVSLDLTRYEYYTIGTYHPDKDRYMPDNT 314

Query: 304 SADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVW 363
           SADGWSGLRYDYGNFYASK+F+DP+K RR+LWGWANESD+VQ DV KGWAGIQ IPR +W
Sbjct: 315 SADGWSGLRYDYGNFYASKTFFDPVKNRRLLWGWANESDAVQSDVQKGWAGIQTIPRKIW 374

Query: 364 LDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQV---IIFSFSSLDN 423
           LD  GKQLLQWP+ EL  LR + V M + KL  G++VEIKGITAAQ    ++FSFSSLD 
Sbjct: 375 LDSYGKQLLQWPVEELETLRGQNVHMSSVKLNNGEHVEIKGITAAQADVDVVFSFSSLDG 434

Query: 424 AEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKA--ETNHVV 483
           AE FD SW++AQ +C   GS   G VGPFGLLTLASE+LEEFTPVFFRIFKA  +  H V
Sbjct: 435 AEQFDSSWLNAQDLCGLKGSGVPGNVGPFGLLTLASEHLEEFTPVFFRIFKADDQNKHKV 494

Query: 484 LMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVY 543
           LMCSDARSSSLK G+YKP+FAGFVD+DLS  K+ LRSLIDHSV+ESFG GGKTCITSRVY
Sbjct: 495 LMCSDARSSSLKSGIYKPSFAGFVDVDLSDGKISLRSLIDHSVVESFGAGGKTCITSRVY 554

Query: 544 PTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSAWVN 576
           PT A+  +A L  FNNGT++IT+E L AWSM    +N
Sbjct: 555 PTLALLGNARLFTFNNGTQTITIETLDAWSMNRPVMN 581