Cp4.1LG12g04140 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG12g04140 vs. Swiss-Prot
Match: INV1_DAUCA (Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota GN=INV1 PE=1 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 8.4e-224
Identity = 370/557 (66.43%), Postives = 438/557 (78.64%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 78
           V+A H+I+  LQS+   N    +KQ+HRTG+HFQP ++W+NDPNGPMY+KG+YHLFYQYN
Sbjct: 37  VEAFHEIHYNLQSVGAEN----VKQVHRTGYHFQPKQNWINDPNGPMYYKGVYHLFYQYN 96

Query: 79  PEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGI 138
           P+GAVWGNIVWAHS+S DLINW  L+PA+ PSKPFD  GC SGSAT+LPG KPVILYTGI
Sbjct: 97  PKGAVWGNIVWAHSVSTDLINWTPLEPAIFPSKPFDKYGCRSGSATILPGNKPVILYTGI 156

Query: 139 ---DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFKGH 198
                +N QVQNYAIPANL DP+L +W+KPDNNP+VV   G N++AFRDP+TAWL   GH
Sbjct: 157 VEGPPKNVQVQNYAIPANLSDPYLRKWIKPDNNPLVVANNGENATAFRDPTTAWLDKSGH 216

Query: 199 WKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFGRFGLDT 258
           WK ++GSK  + GIAYLY+SKDF+ WTK + P+HS  N+GMWECPDF+PVS  G  GLDT
Sbjct: 217 WKMLVGSKRNRRGIAYLYRSKDFIKWTKAKHPIHSQANTGMWECPDFFPVSLKGLNGLDT 276

Query: 259 SVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKS 318
           SVTG  VKHVLKVSL++TRYEYYTVG Y    D+Y+PDNTS DGW+GLRYDYGNFYASK+
Sbjct: 277 SVTGESVKHVLKVSLDLTRYEYYTVGTYLTDKDRYIPDNTSVDGWAGLRYDYGNFYASKT 336

Query: 319 FYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLR 378
           F+DP K RR+LWGWANESDS   DV+KGWAGIQ IPRT+WLDPSGKQL+QWP+ EL  LR
Sbjct: 337 FFDPSKNRRILWGWANESDSTAHDVAKGWAGIQLIPRTLWLDPSGKQLMQWPIEELETLR 396

Query: 379 -------RK-----------------RADVEVIFSFSSLDKAEVFDSSWV--DAQAVCSQ 438
                  RK                 +ADVEV FSF SL K E FD  W+  DA+ +CS 
Sbjct: 397 GSKVKFSRKQDLSKGILVEVKGITAAQADVEVTFSFKSLAKREPFDPKWLEYDAEKICSL 456

Query: 439 LGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSLKDGLY 498
            GST QGGVGPFGLLTLASE LEE+TPVFFR+FK    +  H VLMCSDA  SSLK+GLY
Sbjct: 457 KGSTVQGGVGPFGLLTLASEKLEEYTPVFFRVFKA---QNTHKVLMCSDATRSSLKEGLY 516

Query: 499 KPTFAGFVDIDLSS-KKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHLHVFN 546
           +P+FAGFVD+DL++ KK+ LRSLID+SV+ESFG  GKTCI+SRVYPT AV+E AHL+VFN
Sbjct: 517 RPSFAGFVDVDLATDKKISLRSLIDNSVVESFGAKGKTCISSRVYPTLAVYENAHLYVFN 576

BLAST of Cp4.1LG12g04140 vs. Swiss-Prot
Match: INV3_DAUCA (Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota GN=INV3 PE=3 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 4.2e-215
Identity = 358/570 (62.81%), Postives = 426/570 (74.74%), Query Frame = 1

Query: 5   FSFYSLFIDNGPVVVQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGP 64
           FSF  L I+     V + H+++PELQS+      +++K +HRTG+HFQP +HW+NDPNGP
Sbjct: 21  FSFIFLNING----VDSTHRVFPELQSIS----AVDVKLVHRTGYHFQPQKHWINDPNGP 80

Query: 65  MYFKGIYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSAT 124
           M++KG YHLFYQYNP+G+VWGNIVWAHS+S+DLINW +L+PA+ PSKPFD  GCWSGSAT
Sbjct: 81  MFYKGYYHLFYQYNPKGSVWGNIVWAHSVSKDLINWIALEPAIFPSKPFDQYGCWSGSAT 140

Query: 125 VLPGFKPVILYTGI---DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSA 184
           +LPG KPVILYTGI   D +N QVQNYA+PAN  DP L EWVKPDNNP+V   T  N SA
Sbjct: 141 ILPGNKPVILYTGIVSPDPENAQVQNYAVPANYSDPFLREWVKPDNNPLVGVHT-ENPSA 200

Query: 185 FRDPSTAWLSFKGHWKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPD 244
           FRDP+TAW    GHWK ++GS  +  GIAYLY+SKDF  W +   P+H+   +GMWECPD
Sbjct: 201 FRDPTTAWFD-GGHWKMLVGSSRKHRGIAYLYRSKDFKKWKRSPHPIHTKAETGMWECPD 260

Query: 245 FYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWS 304
           FYPVS     GLD S  G  +KHVLKVSL  TRYEYYT+G+Y  + D YVPDNTS DGW+
Sbjct: 261 FYPVSPRSEDGLDNSKMGRGIKHVLKVSLNSTRYEYYTIGRYNRVRDFYVPDNTSVDGWA 320

Query: 305 GLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGK 364
           GLRYDYGNFYASK+FYDP+KKRR+LWGWANESDS  DDV KGWAGIQ IPR +WLDPSG+
Sbjct: 321 GLRYDYGNFYASKTFYDPIKKRRILWGWANESDSQIDDVQKGWAGIQLIPRRIWLDPSGR 380

Query: 365 QLLQWPVMELNRLR-----------------------RKRADVEVIFSFSSLDKAEVFDS 424
           QL+QWP+ E+  LR                         +ADV+  FSF SLDKAE FD 
Sbjct: 381 QLVQWPIEEVEGLRGSELHMRNQKLDMGVHVEVTGITAAQADVDATFSFKSLDKAESFDP 440

Query: 425 SWV--DAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMC 484
            W+  DAQ VC  +GST QGG+GPFGLLTLAS++LEE+TPVFFRIFK E  +    VLMC
Sbjct: 441 EWINLDAQDVCDSMGSTIQGGLGPFGLLTLASKDLEEYTPVFFRIFKAEDQKLK--VLMC 500

Query: 485 SDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTK 544
           SDA+ SSL +GLYKP+F GFVD+DLS KK+ LRSLID+SV+ESFG   K  I+SRVYPT 
Sbjct: 501 SDAKRSSLAEGLYKPSFRGFVDVDLSDKKISLRSLIDNSVVESFGAQRKNLISSRVYPTL 560

Query: 545 AVFEAAHLHVFNNGTESITVEYLSAWSMRS 547
           A++  AHL VFNNGTE ITV+ L AWSM S
Sbjct: 561 AIYNNAHLFVFNNGTEPITVDNLDAWSMNS 578

BLAST of Cp4.1LG12g04140 vs. Swiss-Prot
Match: INV2_DAUCA (Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota GN=INV2 PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 3.3e-212
Identity = 353/558 (63.26%), Postives = 428/558 (76.70%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 78
           V+A H+++PELQS+      + ++ +HRTG+HFQP +HW+NDPNGPMY+KG YHLFYQYN
Sbjct: 38  VEASHQVFPELQSV----SAVNVQLVHRTGYHFQPKKHWINDPNGPMYYKGFYHLFYQYN 97

Query: 79  PEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGI 138
           P+GAVWGNIVWAHSIS+DLINW +L+PA+ PSKPFD  GCWSGSATVLPG KPVI+YTGI
Sbjct: 98  PKGAVWGNIVWAHSISKDLINWVALEPAIFPSKPFDKYGCWSGSATVLPGGKPVIMYTGI 157

Query: 139 ---DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFKG- 198
                 N QVQN+A+PAN  DP+L EW+KPDNNPI V     NSS+FRDP+TAW  F G 
Sbjct: 158 VTPSPVNTQVQNFAVPANYSDPYLREWIKPDNNPI-VRARSENSSSFRDPTTAW--FDGV 217

Query: 199 HWKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFGRFGLD 258
           HWK ++GS+ +  GIAYLY+S++FL WTK + PLHS   +GMWEC DFYPV+  G  GLD
Sbjct: 218 HWKILVGSRRKHRGIAYLYRSRNFLKWTKAKHPLHSKDRTGMWECLDFYPVAPKGMNGLD 277

Query: 259 TSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASK 318
           TSVTG  +KHVLKVSL  TRYEYYTVG+Y    D YVPDNTS DGW+GLRYDYGNFYASK
Sbjct: 278 TSVTGQDIKHVLKVSLYSTRYEYYTVGEYDRDNDIYVPDNTSVDGWAGLRYDYGNFYASK 337

Query: 319 SFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRL 378
           +F+DP K+RR+LWGWANESDS QDDV KGWAGIQ IPR +WLDP+GKQL+QWP+ E+  L
Sbjct: 338 TFFDPDKQRRILWGWANESDSKQDDVQKGWAGIQLIPRKLWLDPNGKQLIQWPIEEIQLL 397

Query: 379 RRK-----------------------RADVEVIFSFSSLDKAEVFDSSW--VDAQAVCSQ 438
           R +                       +ADV+  FSF SLD+AE FD +W  +DA  VC  
Sbjct: 398 RGQNVHMGSQVLNTGEHIEVKGVTAAQADVDATFSFKSLDRAEWFDPNWAKLDALDVCDW 457

Query: 439 LGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDA-RSSSLKDGL 498
           +GST +GG+GPFG LTLASE LEE+TPVFFR+FK    +    VLMCSDA RSS+  +GL
Sbjct: 458 MGSTVRGGLGPFGFLTLASEKLEEYTPVFFRVFK---TKDKLKVLMCSDAKRSSTTAEGL 517

Query: 499 YKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHLHVFN 547
           YKP FAG+VD+DLS KK+ LRSLID+SV+ESFG  G+TCITSRVYP  A++  AH+ VFN
Sbjct: 518 YKPPFAGYVDVDLSDKKISLRSLIDNSVVESFGAHGRTCITSRVYPKIAIYNNAHVFVFN 577

BLAST of Cp4.1LG12g04140 vs. Swiss-Prot
Match: INV4_ARATH (Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana GN=CWINV4 PE=2 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 5.1e-197
Identity = 323/560 (57.68%), Postives = 414/560 (73.93%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIE-IKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQY 78
           + A H+IY ELQS     +++E +  LHR  FHFQP +HW+NDPNGP+Y+KG+YHLFYQY
Sbjct: 27  IDAFHQIYEELQS-----ESVESVNHLHRPSFHFQPPKHWINDPNGPVYYKGLYHLFYQY 86

Query: 79  NPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTG 138
           N +GAVWGNI+WAHS+S+DL+NW++L+PALSPSK FDI G WSGS T++PG  P+ILYTG
Sbjct: 87  NTKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTG 146

Query: 139 IDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFKGHWK 198
           ++    Q+QNYAIP +  DP+L +W+KPD+NPI +P   +N SAFRDP+TAW S  GHW+
Sbjct: 147 VNQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTMNGSAFRDPTTAWFSKDGHWR 206

Query: 199 TIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFG-RFGLDTS 258
           T++GSK ++ GIAY+Y+S+DF +W K + P+HS  ++GMWECPDF+PVS    R GLD  
Sbjct: 207 TVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMWECPDFFPVSLTDFRNGLDLD 266

Query: 259 VTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSF 318
             G   KHVLKVSL++TRYEYYT+GKY    D+Y+PD  + DGW GLR+DYGNFYASK+F
Sbjct: 267 YVGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNTPDGWEGLRFDYGNFYASKTF 326

Query: 319 YDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRR 378
           +D  K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV LD S KQL+ WPV E+  LR 
Sbjct: 327 FDYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLLDSSKKQLVFWPVEEIESLRG 386

Query: 379 K-----------------------RADVEVIFSFSSLDKAEVFDSSWV-DAQAVCSQLGS 438
                                   +ADVEV F   SL+KAE+FD S+      +C+  GS
Sbjct: 387 NYVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEIFDPSFTWKPLELCNIKGS 446

Query: 439 TAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSL-------- 498
             +GGVGPFGL+TLA+ +LEE+TPVFFR+F  +T      VLMCSDAR SSL        
Sbjct: 447 NVRGGVGPFGLITLATPDLEEYTPVFFRVF-NDTKTHKPKVLMCSDARPSSLKQDTGLLA 506

Query: 499 KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHL 545
           KD +YKP+FAGFVD+D++  ++ LRSLIDHSV+ESFG  GKT ITSRVYP KAV E AHL
Sbjct: 507 KDRMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGKTVITSRVYPVKAVKENAHL 566

BLAST of Cp4.1LG12g04140 vs. Swiss-Prot
Match: INV2_ARATH (Beta-fructofuranosidase, insoluble isoenzyme CWINV2 OS=Arabidopsis thaliana GN=CWINV2 PE=2 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 2.0e-193
Identity = 325/561 (57.93%), Postives = 403/561 (71.84%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 78
           V A HK++ +LQS  K      +  LHRT +HFQP RHW+NDPN PM +KG+YHLFYQYN
Sbjct: 23  VDAFHKVFKKLQS--KSTSLESVSPLHRTAYHFQPPRHWINDPNAPMLYKGVYHLFYQYN 82

Query: 79  PEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGI 138
           P+GAVWGNIVWAHS+S+DLINW++L+PA+ PSK FDING WSGSAT +PG  PVILYTGI
Sbjct: 83  PKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTGI 142

Query: 139 DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFK-GHWK 198
                Q+QNYAIP +L DP+L  W+KPD+NPIV P  G N SAFRDP+TAW + K G+W+
Sbjct: 143 TENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRDPTTAWFNKKDGYWR 202

Query: 199 TIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFG-RFGLDTS 258
            ++GSK +  GIAY+YKS+DF  W K ++P+HS   +GMWECPDF+PVS    + GLD S
Sbjct: 203 MLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFFPVSVTDKKNGLDFS 262

Query: 259 VTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSF 318
             G   KHVLKVSL++TRYEYYT+G Y    D+Y PD  + DGW GLR+DYGN+YASK+F
Sbjct: 263 YDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDGLRFDYGNYYASKTF 322

Query: 319 YDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRR 378
           +D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT+ LD SGKQL+ WP+ E+  LR 
Sbjct: 323 FDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQLVFWPIEEIESLRG 382

Query: 379 K-----------------------RADVEVIFSFSSLDKAEVFDSSWVDAQ-AVCSQLGS 438
           K                       + DV+V F+  +L+KAE FD S+      +C+  GS
Sbjct: 383 KNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDESFATKPLELCNLKGS 442

Query: 439 TAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSL-------- 498
              GGVGPFGL+TLA+ +LEE+TPVFFR+FK +       VLMCSDA+ SSL        
Sbjct: 443 NVNGGVGPFGLITLATSDLEEYTPVFFRVFK-DAASNKPKVLMCSDAKPSSLKKDTGTDA 502

Query: 499 KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHL 546
           K+ +YKP+FAGFVD+ L   K+ LRSLIDHSV+ESFG  GKT ITSRVYPTKAV E AHL
Sbjct: 503 KERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSRVYPTKAVGEKAHL 562

BLAST of Cp4.1LG12g04140 vs. TrEMBL
Match: A0A0A0LAY1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G777580 PE=3 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 7.3e-267
Identity = 443/574 (77.18%), Postives = 485/574 (84.49%), Query Frame = 1

Query: 1   ILLGFSFYSLFIDNGPVVVQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMND 60
           +LLGFSF+SLFI+NG V V ALHKIYPELQS++   D   IK LHRT FHFQP R+W+ND
Sbjct: 9   LLLGFSFFSLFINNGVVFVDALHKIYPELQSIQ--TDASVIKPLHRTRFHFQPRRNWIND 68

Query: 61  PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWS 120
           PNGPMYFKGIYHLFYQYNP+GAVWGNIVWAHSISRDLINWK LKPA+ PSKPFDINGCWS
Sbjct: 69  PNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKPAIYPSKPFDINGCWS 128

Query: 121 GSATVLPGFKPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSS 180
           GSATVLPG KPVILYTGID QNRQVQNYAIPANL DP+LTEW+KPDNNPIV PG GVN+S
Sbjct: 129 GSATVLPGDKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDNNPIVDPGPGVNAS 188

Query: 181 AFRDPSTAWLSFKGHWKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECP 240
           AFRDP+TAWLS  GHWKT+IGSK ++ G+AYLY+S+DF+ WTK + PLHS  N+GMWECP
Sbjct: 189 AFRDPTTAWLSKSGHWKTVIGSKRKKRGMAYLYRSRDFVKWTKAKHPLHSAANTGMWECP 248

Query: 241 DFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGW 300
           DFYPV   G+ GLD SVTGNWVKHV KVSL++TRYEYYTVGKY+P  DKYVPDNTS DGW
Sbjct: 249 DFYPVPLRGKLGLDPSVTGNWVKHVFKVSLDLTRYEYYTVGKYFPKKDKYVPDNTSVDGW 308

Query: 301 SGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSG 360
           SGLRYDYGNFYASKSFYDP+KKRRVLWGWANESDS QDDVSKGWAGIQ IPRTVWLD + 
Sbjct: 309 SGLRYDYGNFYASKSFYDPLKKRRVLWGWANESDSAQDDVSKGWAGIQLIPRTVWLDHNQ 368

Query: 361 KQLLQWPVMELNRLRRKR-----------------------ADVEVIFSFSSLDKAEVFD 420
           +QL QWPV ELN LR K+                       ADVEV+FSFSSLDKA+ FD
Sbjct: 369 RQLKQWPVKELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVMFSFSSLDKADPFD 428

Query: 421 SSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCS 480
             WVDAQA C QLG++ QGGVGPFGLLTLASENL+EFTPVFFRIFK       HVVLMCS
Sbjct: 429 PRWVDAQAACDQLGNS-QGGVGPFGLLTLASENLDEFTPVFFRIFKA---HHKHVVLMCS 488

Query: 481 DARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTK 540
           DARSSSLK +GLYKP FAG+VDIDLS KKL LRSLIDHSV+ESFGGGGKTCITSRVYPTK
Sbjct: 489 DARSSSLKEEGLYKPAFAGYVDIDLSRKKLSLRSLIDHSVVESFGGGGKTCITSRVYPTK 548

Query: 541 AVFEAAHLHVFNNGTESITVEYLSAWSMRSAWVN 551
            VF+ AHLHVFNNGTE+ITVEYL AWSM+SA +N
Sbjct: 549 GVFDDAHLHVFNNGTEAITVEYLRAWSMKSARMN 576

BLAST of Cp4.1LG12g04140 vs. TrEMBL
Match: A0A059DIE8_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02647 PE=3 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 8.4e-231
Identity = 371/555 (66.85%), Postives = 441/555 (79.46%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 78
           V+A HK+YPE QSL      + +K  HRTG+HFQP +HW+NDPNGPM++KG YHLFYQYN
Sbjct: 33  VEARHKVYPEYQSLP----AVSVKYAHRTGYHFQPPKHWINDPNGPMHYKGWYHLFYQYN 92

Query: 79  PEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGI 138
           P+GAVWGNIVWAHS+S+DL+NW++L PA+ P+KPFDINGCWSGSATVLPG +P+ILYTGI
Sbjct: 93  PKGAVWGNIVWAHSVSKDLVNWRALPPAIYPTKPFDINGCWSGSATVLPGNRPIILYTGI 152

Query: 139 DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFKGHWKT 198
           D++N QVQNYA+PAN  DP+L EW KPDNNPIV PG GVN+SAFRDP+TAW    GHWK 
Sbjct: 153 DTKNHQVQNYAVPANASDPYLREWTKPDNNPIVDPGPGVNASAFRDPTTAWFE-DGHWKI 212

Query: 199 IIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFGRFGLDTSVT 258
           ++GS+ +  G+AYLY+S+DF++WTK + PLHS P +GMWECPDF+PVS     GLDTS  
Sbjct: 213 LVGSRRKHRGMAYLYRSRDFIHWTKAKHPLHSAPKTGMWECPDFFPVSKNNANGLDTSAL 272

Query: 259 GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYD 318
           G  VKHVLKVSL++TRYEYYT+G Y+P  D+Y+PDNTSADGWSGLRYDYGNFYASK+F+D
Sbjct: 273 GQGVKHVLKVSLDLTRYEYYTIGTYHPDKDRYMPDNTSADGWSGLRYDYGNFYASKTFFD 332

Query: 319 PMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRRK- 378
           P+K RR+LWGWANESD+VQ DV KGWAGIQ IPR +WLD  GKQLLQWPV EL  LR + 
Sbjct: 333 PVKNRRLLWGWANESDAVQSDVQKGWAGIQTIPRKIWLDSYGKQLLQWPVEELETLRGQN 392

Query: 379 ----------------------RADVEVIFSFSSLDKAEVFDSSWVDAQAVCSQLGSTAQ 438
                                 +ADV+V+FSFSSLD AE FDSSW++AQ +C   GS   
Sbjct: 393 VHMSSVKLNNGEHVEIKGITAAQADVDVVFSFSSLDGAEQFDSSWLNAQDLCGLKGSGVP 452

Query: 439 GGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSLKDGLYKPTFAG 498
           G VGPFGLLTLASE+LEEFTPVFFRIFK + ++  H VLMCSDARSSSLK G+YKP+FAG
Sbjct: 453 GNVGPFGLLTLASEHLEEFTPVFFRIFKAD-DQNKHKVLMCSDARSSSLKSGIYKPSFAG 512

Query: 499 FVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHLHVFNNGTESIT 551
           FVD+DLS  K+ LRSLIDHSV+ESFG GGKTCITSRVYPT A+   A L  FNNGT++IT
Sbjct: 513 FVDVDLSDGKISLRSLIDHSVVESFGAGGKTCITSRVYPTLALLGNARLFTFNNGTQTIT 572

BLAST of Cp4.1LG12g04140 vs. TrEMBL
Match: A0A0N6XFM9_RHUCH (Cell wall invertase OS=Rhus chinensis PE=2 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 2.1e-229
Identity = 371/557 (66.61%), Postives = 438/557 (78.64%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 78
           ++A H +YPE QSL      + +KQ HRTGFHFQP +HW+NDPNGPMY+ GIYH FYQYN
Sbjct: 24  IEASHVVYPEFQSLA----AVTVKQTHRTGFHFQPPKHWINDPNGPMYYNGIYHFFYQYN 83

Query: 79  PEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGI 138
           P+GAVWGNIVWAHS+SRD+INW++L+PA+ PSKPFDI GCWSGS T+LPG KP+ILYTGI
Sbjct: 84  PKGAVWGNIVWAHSVSRDMINWQALEPAIYPSKPFDIKGCWSGSVTILPGNKPIILYTGI 143

Query: 139 DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFKGHWKT 198
           DS+ RQ+Q+YA+P NL DP+L EWVKPDNNP+V P  GVN+SAFRDP+TAW    GHW+ 
Sbjct: 144 DSEERQLQDYAVPKNLSDPYLREWVKPDNNPLVFPH-GVNASAFRDPTTAWWEDDGHWRM 203

Query: 199 IIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFGRFGLDTSVT 258
           ++GS+ +Q GIAYLY+SKDF+ WTK + P+HS   +GMWECPDF+PVS  G  GLDTS  
Sbjct: 204 LVGSRRKQRGIAYLYRSKDFMKWTKAKHPIHSKEKTGMWECPDFFPVSLSGTNGLDTSYR 263

Query: 259 GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYD 318
           G  +K+V K+SL+MTRY+YYTVG Y+   D+YVPDNTS DGW GLRYDYGNFYASK+F+D
Sbjct: 264 GPNLKYVFKISLDMTRYDYYTVGSYFRDKDRYVPDNTSVDGWDGLRYDYGNFYASKTFFD 323

Query: 319 PMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRRK- 378
             K RR+LWGWANESDS  DDV KGWAG+QAIPR++WLDPSGKQ+LQWP+ EL  LR K 
Sbjct: 324 AGKNRRILWGWANESDSKDDDVDKGWAGVQAIPRSLWLDPSGKQVLQWPIQELETLRGKN 383

Query: 379 -----------------------RADVEVIFSFSSLDKAEVFDSSWVDAQAVCSQLGSTA 438
                                  +ADVEV FSF SLDKAE FD SWV+A+ +C Q GST 
Sbjct: 384 AEISNKVLQSGENVEVGQGITAAQADVEVTFSFPSLDKAEPFDPSWVNAEKLCGQKGSTV 443

Query: 439 QGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSLK-DGLYKPTF 498
           +GGVGPFGLLTLASE LEE+TPVFFRIFK       HVVLMCSDA SSSLK  GLYKP+F
Sbjct: 444 KGGVGPFGLLTLASEKLEEYTPVFFRIFKA---PNKHVVLMCSDATSSSLKTTGLYKPSF 503

Query: 499 AGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHLHVFNNGTES 551
           AGFVD+DL+ KKL LRSLIDHSV+ESFG GGKTCITSRVYPT AVF  AHL  FNNGTE+
Sbjct: 504 AGFVDVDLADKKLSLRSLIDHSVVESFGAGGKTCITSRVYPTLAVFSNAHLFAFNNGTET 563

BLAST of Cp4.1LG12g04140 vs. TrEMBL
Match: A0A067FSV9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g008189mg PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 5.3e-225
Identity = 369/553 (66.73%), Postives = 439/553 (79.39%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 78
           V+A HKIYPE QSL      +++KQLHRT FHFQP +HW+N   GPMY+KGIYHLFYQYN
Sbjct: 25  VEASHKIYPEFQSLA----AVKVKQLHRTAFHFQPPKHWIN---GPMYYKGIYHLFYQYN 84

Query: 79  PEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGI 138
           P+GAVWGNIVWAHS+S+DLINW++L+PAL PSKPFDINGCWSGSATVLPG KP+ILYTG+
Sbjct: 85  PKGAVWGNIVWAHSVSKDLINWEALEPALYPSKPFDINGCWSGSATVLPGNKPIILYTGV 144

Query: 139 DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFKGHWKT 198
           D + RQVQNYA+PAN  DP+L +W+KPDNNP+V PG  VN+SAFRDP+TAW S  GHW+ 
Sbjct: 145 DHKERQVQNYAVPANPSDPYLRKWIKPDNNPVVFPGPDVNASAFRDPTTAWWSNDGHWRM 204

Query: 199 IIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFGRFGLDTSVT 258
           ++GS+ +  G+AYLY+S+DF+ WTK + P+HS+ N+GMWECPDFYPVS  G+ GLDTS  
Sbjct: 205 LVGSRRKHRGMAYLYRSRDFMKWTKAKHPIHSLANTGMWECPDFYPVSISGKNGLDTSFA 264

Query: 259 GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYD 318
           G   K VLKVSL++TRY+YYT+G Y    D+YVPD  S DGW GLRYDYGNFYASK+F+D
Sbjct: 265 GGNEKFVLKVSLDLTRYDYYTIGTYNRDKDRYVPDKDSVDGWRGLRYDYGNFYASKTFFD 324

Query: 319 PMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRRK- 378
             K RR+LWGWANESDS  DD++KGWAG+Q IPR VWLD SGKQLLQWP+ EL  LR K 
Sbjct: 325 SRKNRRILWGWANESDSTFDDMAKGWAGVQTIPREVWLDGSGKQLLQWPIEELETLRGKN 384

Query: 379 ----------------------RADVEVIFSFSSLDKAEVFDSSW--VDAQAVCSQLGST 438
                                 +ADVEV F   SLDKAE FD SW  +DA+ +C ++GS 
Sbjct: 385 VHISNQELKKGHHVEVKGVTAAQADVEVTFKLPSLDKAEKFDPSWKNLDAEHLCGKIGSK 444

Query: 439 AQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSLK-DGLYKPT 498
            QGGVGPFGLLTLAS+NLEEFTPVFFRIFK   +   H+VLMCSDA SS+LK +GLYKP+
Sbjct: 445 VQGGVGPFGLLTLASKNLEEFTPVFFRIFK---DGHKHLVLMCSDASSSTLKTEGLYKPS 504

Query: 499 FAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHLHVFNNGTE 546
           FAGFV++DLS KKL LRSLIDHSV+ESFG GGKTCITSRVYPT AVF+ AHL+ FNNGTE
Sbjct: 505 FAGFVNVDLSDKKLSLRSLIDHSVVESFGAGGKTCITSRVYPTLAVFDDAHLYAFNNGTE 564

BLAST of Cp4.1LG12g04140 vs. TrEMBL
Match: A0A061EUI0_THECC (Cell wall invertase 2 isoform 1 OS=Theobroma cacao GN=TCM_022641 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 7.6e-224
Identity = 357/555 (64.32%), Postives = 437/555 (78.74%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 78
           V A HK++P+ QSL    + + ++Q+HRTG+HFQP ++W+NDPN PMY+ GIYH FYQYN
Sbjct: 28  VDASHKVFPQFQSL----NAVSVQQIHRTGYHFQPPKNWINDPNAPMYYDGIYHFFYQYN 87

Query: 79  PEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGI 138
           P+GAVWGNIVWAHS+S+D+INW++L+PAL PS+ FDINGCWSGSATVLP  KP+ILYTG+
Sbjct: 88  PKGAVWGNIVWAHSVSKDMINWQALEPALYPSQHFDINGCWSGSATVLPSNKPIILYTGL 147

Query: 139 DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFKGHWKT 198
           D ++RQ+QNYA+PANL DP+L +W+KP NNP+V P  GVN+SAFRDP+TAW    GHW+ 
Sbjct: 148 DPKDRQIQNYAVPANLSDPYLRKWIKPGNNPVVDPEQGVNASAFRDPTTAWW-LNGHWRM 207

Query: 199 IIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFGRFGLDTSVT 258
           ++GSK    G+AYLY+S+DF+ WTK + PLHS P++GMWECPDFYPV   G+ GLD SV 
Sbjct: 208 LVGSKRNHRGMAYLYRSRDFIYWTKAKHPLHSAPHTGMWECPDFYPVLLSGKKGLDPSVL 267

Query: 259 GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYD 318
           G  VKHVLKVSL++TRY+YYTVG Y    D+YVPDNTS DGW+GLRYDYGNFYASK+F+D
Sbjct: 268 GKHVKHVLKVSLDVTRYDYYTVGTYLSDKDRYVPDNTSVDGWAGLRYDYGNFYASKTFFD 327

Query: 319 PMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRRK- 378
           P KKRR+LWGW NESD+ +DDV KGWAG+QAIPR VWLDP+ +QL+QWP+ EL  LR K 
Sbjct: 328 PAKKRRILWGWVNESDTTEDDVKKGWAGLQAIPRIVWLDPNQRQLMQWPIEELETLRGKN 387

Query: 379 ----------------------RADVEVIFSFSSLDKAEVFDSSWVDAQAVCSQLGSTAQ 438
                                 +ADV+V F   +LDKAE FD SW DAQ +C + GS  +
Sbjct: 388 VKMSHQQLKKGEHIEVKGITAAQADVDVTFHIPNLDKAEPFDHSWTDAQELCVKKGSKVK 447

Query: 439 GGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSLKDGLYKPTFAG 498
           G VGPFGLLTLAS+NLEE+TPV FR+F+       HVVL+CSDARSSSLKDGLYKP+FAG
Sbjct: 448 GEVGPFGLLTLASKNLEEYTPVSFRVFQ---TSKKHVVLLCSDARSSSLKDGLYKPSFAG 507

Query: 499 FVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHLHVFNNGTESIT 551
           FVD+DL+ KKL LRSLIDHSV+ESFG GGKTCITSRVYPT AV + AHL+ FN+GTE I 
Sbjct: 508 FVDVDLADKKLSLRSLIDHSVVESFGAGGKTCITSRVYPTLAVLDNAHLYAFNHGTEPIN 567

BLAST of Cp4.1LG12g04140 vs. TAIR10
Match: AT2G36190.1 (AT2G36190.1 cell wall invertase 4)

HSP 1 Score: 688.7 bits (1776), Expect = 2.9e-198
Identity = 323/560 (57.68%), Postives = 414/560 (73.93%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIE-IKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQY 78
           + A H+IY ELQS     +++E +  LHR  FHFQP +HW+NDPNGP+Y+KG+YHLFYQY
Sbjct: 27  IDAFHQIYEELQS-----ESVESVNHLHRPSFHFQPPKHWINDPNGPVYYKGLYHLFYQY 86

Query: 79  NPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTG 138
           N +GAVWGNI+WAHS+S+DL+NW++L+PALSPSK FDI G WSGS T++PG  P+ILYTG
Sbjct: 87  NTKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTG 146

Query: 139 IDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFKGHWK 198
           ++    Q+QNYAIP +  DP+L +W+KPD+NPI +P   +N SAFRDP+TAW S  GHW+
Sbjct: 147 VNQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTMNGSAFRDPTTAWFSKDGHWR 206

Query: 199 TIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFG-RFGLDTS 258
           T++GSK ++ GIAY+Y+S+DF +W K + P+HS  ++GMWECPDF+PVS    R GLD  
Sbjct: 207 TVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMWECPDFFPVSLTDFRNGLDLD 266

Query: 259 VTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSF 318
             G   KHVLKVSL++TRYEYYT+GKY    D+Y+PD  + DGW GLR+DYGNFYASK+F
Sbjct: 267 YVGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNTPDGWEGLRFDYGNFYASKTF 326

Query: 319 YDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRR 378
           +D  K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV LD S KQL+ WPV E+  LR 
Sbjct: 327 FDYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLLDSSKKQLVFWPVEEIESLRG 386

Query: 379 K-----------------------RADVEVIFSFSSLDKAEVFDSSWV-DAQAVCSQLGS 438
                                   +ADVEV F   SL+KAE+FD S+      +C+  GS
Sbjct: 387 NYVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEIFDPSFTWKPLELCNIKGS 446

Query: 439 TAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSL-------- 498
             +GGVGPFGL+TLA+ +LEE+TPVFFR+F  +T      VLMCSDAR SSL        
Sbjct: 447 NVRGGVGPFGLITLATPDLEEYTPVFFRVF-NDTKTHKPKVLMCSDARPSSLKQDTGLLA 506

Query: 499 KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHL 545
           KD +YKP+FAGFVD+D++  ++ LRSLIDHSV+ESFG  GKT ITSRVYP KAV E AHL
Sbjct: 507 KDRMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGKTVITSRVYPVKAVKENAHL 566

BLAST of Cp4.1LG12g04140 vs. TAIR10
Match: AT3G52600.1 (AT3G52600.1 cell wall invertase 2)

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-194
Identity = 325/561 (57.93%), Postives = 403/561 (71.84%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 78
           V A HK++ +LQS  K      +  LHRT +HFQP RHW+NDPN PM +KG+YHLFYQYN
Sbjct: 23  VDAFHKVFKKLQS--KSTSLESVSPLHRTAYHFQPPRHWINDPNAPMLYKGVYHLFYQYN 82

Query: 79  PEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGI 138
           P+GAVWGNIVWAHS+S+DLINW++L+PA+ PSK FDING WSGSAT +PG  PVILYTGI
Sbjct: 83  PKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTGI 142

Query: 139 DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFK-GHWK 198
                Q+QNYAIP +L DP+L  W+KPD+NPIV P  G N SAFRDP+TAW + K G+W+
Sbjct: 143 TENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRDPTTAWFNKKDGYWR 202

Query: 199 TIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFG-RFGLDTS 258
            ++GSK +  GIAY+YKS+DF  W K ++P+HS   +GMWECPDF+PVS    + GLD S
Sbjct: 203 MLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFFPVSVTDKKNGLDFS 262

Query: 259 VTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSF 318
             G   KHVLKVSL++TRYEYYT+G Y    D+Y PD  + DGW GLR+DYGN+YASK+F
Sbjct: 263 YDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDGLRFDYGNYYASKTF 322

Query: 319 YDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRR 378
           +D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT+ LD SGKQL+ WP+ E+  LR 
Sbjct: 323 FDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQLVFWPIEEIESLRG 382

Query: 379 K-----------------------RADVEVIFSFSSLDKAEVFDSSWVDAQ-AVCSQLGS 438
           K                       + DV+V F+  +L+KAE FD S+      +C+  GS
Sbjct: 383 KNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDESFATKPLELCNLKGS 442

Query: 439 TAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSL-------- 498
              GGVGPFGL+TLA+ +LEE+TPVFFR+FK +       VLMCSDA+ SSL        
Sbjct: 443 NVNGGVGPFGLITLATSDLEEYTPVFFRVFK-DAASNKPKVLMCSDAKPSSLKKDTGTDA 502

Query: 499 KDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHL 546
           K+ +YKP+FAGFVD+ L   K+ LRSLIDHSV+ESFG  GKT ITSRVYPTKAV E AHL
Sbjct: 503 KERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSRVYPTKAVGEKAHL 562

BLAST of Cp4.1LG12g04140 vs. TAIR10
Match: AT3G13790.1 (AT3G13790.1 Glycosyl hydrolases family 32 protein)

HSP 1 Score: 650.6 bits (1677), Expect = 8.7e-187
Identity = 312/571 (54.64%), Postives = 394/571 (69.00%), Query Frame = 1

Query: 9   SLFIDNGPVVVQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFK 68
           +L I N  V ++A H +Y  L        +  + Q +RTGFHFQP ++WMNDPNGPM +K
Sbjct: 16  TLLIGNYVVNLEASHHVYKRLTQ-STNTKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIYK 75

Query: 69  GIYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPG 128
           GIYHLFYQ+NP+GAVWGNIVWAHS S DLINW    PA+ PS PFDINGCWSGSAT+LP 
Sbjct: 76  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 135

Query: 129 FKPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPG--TGVNSSAFRDPS 188
            KPVILYTGID +N+QVQN A P NL DP+L EW K   NP++ P    G+N+S+FRDP+
Sbjct: 136 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 195

Query: 189 TAWLSFKGHWKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVS 248
           TAWL     W+ IIGSK  + G+A  Y SKDFL W K  +PLH    SGMWECPDF+PV+
Sbjct: 196 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 255

Query: 249 HFGRFGLDTSVTG---NWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGL 308
            FG  G++TS  G     +KHVLK+SL+ T+++YYT+G Y  + DK+VPDN      +  
Sbjct: 256 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 315

Query: 309 RYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQL 368
           RYDYG +YASK+F+D  K RR+LWGW NES SV+DDV KGW+GIQ IPR +WLD SGKQL
Sbjct: 316 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 375

Query: 369 LQWPVMELNRLRRK------------------------RADVEVIFSFSSLDKAEVFDSS 428
           +QWPV E+ RLR K                        +ADVEV+F    L+KA+V + S
Sbjct: 376 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 435

Query: 429 WVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDA 488
           W D Q +CS++  + + G+GPFGL+ LAS+NLEE+T V+FRIFK   N   +VVLMCSD 
Sbjct: 436 WTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQ 495

Query: 489 RSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVF 548
             SSLK+   K T+  FVDI+   + L LR+LIDHSV+ESFGG G+ CITSRVYP  A+ 
Sbjct: 496 SRSSLKEDNDKTTYGAFVDIN-PHQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIG 555

Query: 549 EAAHLHVFNNGTESITVEYLSAWSMRSAWVN 551
           +++HL  FN G +S+ V  L+AWSM SA ++
Sbjct: 556 KSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of Cp4.1LG12g04140 vs. TAIR10
Match: AT1G55120.1 (AT1G55120.1 beta-fructofuranosidase 5)

HSP 1 Score: 615.1 bits (1585), Expect = 4.1e-176
Identity = 295/542 (54.43%), Postives = 374/542 (69.00%), Query Frame = 1

Query: 40  EIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWG-NIVWAHSISRDLI 99
           ++ Q +RTG+HFQP ++WMNDPNGPM +KGIYHLFYQYNP GAVW   IVW HS S DL+
Sbjct: 33  DLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLV 92

Query: 100 NWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGIDSQNRQVQNYAIPANLFDPH 159
           NW S  PA +PS+P DINGCWSGS T+LP  KPVILYTGID    QVQN A+P N+ DP+
Sbjct: 93  NWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPY 152

Query: 160 LTEWVKPDNNPIVVPGT--GVNSSAFRDPSTAWLSFKGHWKTIIGSKSE-QTGIAYLYKS 219
           L EW KP  NP++      G+N   FRDP+TAWL   G W+ I+GS ++ + G+A LYKS
Sbjct: 153 LREWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKS 212

Query: 220 KDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFGRFGLDTSVTG-NWVKHVLKVSLEMTR 279
           +DF NWT+  +PLH    +GMWECPDF+PVS  G  G++TS  G N +KHVLKVSL  T 
Sbjct: 213 RDFFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETL 272

Query: 280 YEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESD 339
           ++YYT+G Y    D YVPD       S  R DYG +YASK+FYD +KKRR+LWGW NES 
Sbjct: 273 HDYYTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESS 332

Query: 340 SVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRRK----------------- 399
             +DD+ KGW+G+Q+ PR +WLD SGK+LLQWP+ E+  LR +                 
Sbjct: 333 PAKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQV 392

Query: 400 ------RADVEVIFSFSSLDKAEVFDSSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENL 459
                 +ADVEV F    L+KA+V + SW D Q +CSQ   +   G+GPFGL+ LAS ++
Sbjct: 393 HGVTAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDM 452

Query: 460 EEFTPVFFRIFKG--ETN-ETNHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPL 519
           EE+T V+FRIFK   +TN +T +VVLMCSD   SSL D   K TF  FV ID S + + L
Sbjct: 453 EEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISL 512

Query: 520 RSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHLHVFNNGTESITVEYLSAWSMRSAW 551
           R+LIDHS++ES+GGGG+TCITSRVYP  A+ E A+L VFN GT+S+ +  LSAWS++SA 
Sbjct: 513 RTLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQ 572

BLAST of Cp4.1LG12g04140 vs. TAIR10
Match: AT3G13784.1 (AT3G13784.1 cell wall invertase 5)

HSP 1 Score: 568.5 bits (1464), Expect = 4.3e-162
Identity = 291/573 (50.79%), Postives = 367/573 (64.05%), Query Frame = 1

Query: 5   FSFYSLFIDNGPVVVQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGP 64
           F   SLF+ +  VVV         L +L+   + I+  Q +RTG+HFQP ++WMN   GP
Sbjct: 12  FLLVSLFLTDDAVVV---------LDALDNVPNNIK-NQPYRTGYHFQPPKNWMN---GP 71

Query: 65  MYFKGIYHLFYQYNPEGAVW--GNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGS 124
           M +KGIYHLFYQ+N  GAV      VW H+ S DLINW +L PA+ PS+P DINGCWSGS
Sbjct: 72  MIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINWITLSPAIRPSRPSDINGCWSGS 131

Query: 125 ATVLPGFKPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPG--TGVNSS 184
            T+LP  KPVILYTG D  NRQVQN   P NL DP+L  W K   NP+V P     +NSS
Sbjct: 132 VTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLRHWTKSPENPLVTPSPVNHINSS 191

Query: 185 AFRDPSTAWLSFKGHWKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECP 244
           AFRDP+TAW    G W+   GS+  + G+A L+ SKDF+ W +  +PLH    +GMWECP
Sbjct: 192 AFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDFVIWKQSPKPLHYHDGTGMWECP 251

Query: 245 DFYPVSHFGRFGLDTSVT-GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADG 304
           DF+PV+     GLDTS + G  VKHVLKVSL  T  +YYT+G Y  + D YVPD      
Sbjct: 252 DFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFNDYYTIGTYDEVRDVYVPDKGFVQD 311

Query: 305 WSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPS 364
            +  RYDYG FYASK+FYD + +RR+LWGW NES   +D++ KGWAG+QAIPR VWLD S
Sbjct: 312 ETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPEKDNIKKGWAGLQAIPRKVWLDES 371

Query: 365 GKQLLQWPVMELNRLR-----------------------RKRADVEVIFSFS--SLDKAE 424
           GK+L+QWPV E+ RLR                         +ADVEV F  S   L+KA+
Sbjct: 372 GKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHGVTAPQADVEVFFKVSGFDLEKAD 431

Query: 425 VFDSSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVL 484
           V +  W D Q +CSQ    A  G+GPFGL+ LAS+N+EE+T V  RIF+   N   HVV+
Sbjct: 432 VIEPGWTDPQLICSQ--RNASSGLGPFGLMVLASKNMEEYTSVNIRIFRAGENSKEHVVV 491

Query: 485 MCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYP 544
           MCSD  +SSL+ G  K T+  F+DI    + + LR+LID S++ESFGG GKTCITSRVYP
Sbjct: 492 MCSDQSTSSLEKGNDKTTYGAFLDIS-PYQPISLRTLIDKSIVESFGGKGKTCITSRVYP 551

Query: 545 TKAVFEAAHLHVFNNGTESITVEYLSAWSMRSA 548
             A+ E  HL  FN G++++ V  LSAWSM+S+
Sbjct: 552 KLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568

BLAST of Cp4.1LG12g04140 vs. NCBI nr
Match: gi|659084391|ref|XP_008442862.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucumis melo])

HSP 1 Score: 931.4 bits (2406), Expect = 7.2e-268
Identity = 448/574 (78.05%), Postives = 485/574 (84.49%), Query Frame = 1

Query: 1   ILLGFSFYSLFIDNGPVVVQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMND 60
           +LLGFSF+SLFI+NG   V+ALHKIYPELQS++   D   +K LHRT FHFQP R+W+ND
Sbjct: 10  LLLGFSFFSLFINNG---VEALHKIYPELQSIQ--TDASVVKPLHRTRFHFQPRRNWIND 69

Query: 61  PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWS 120
           PNGPMYF GIYHLFYQYNP+GAVWGNIVWAHSISRDLINWK LK A+ PSKPFDINGCWS
Sbjct: 70  PNGPMYFNGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKAAIYPSKPFDINGCWS 129

Query: 121 GSATVLPGFKPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSS 180
           GSATVLPG KPVILYTGID QNRQVQNYAIPANL DP+LTEW+KPD+NPIV PG GVN+S
Sbjct: 130 GSATVLPGNKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDHNPIVDPGPGVNAS 189

Query: 181 AFRDPSTAWLSFKGHWKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECP 240
           AFRDP+TAWLS  GHWKTIIGSK ++ G+AYLY+S+DF+ WTK + PLHS  N+GMWECP
Sbjct: 190 AFRDPTTAWLSKSGHWKTIIGSKRKRRGMAYLYRSRDFVKWTKAKHPLHSAANTGMWECP 249

Query: 241 DFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGW 300
           DFYPV   GR GLD SVTGNWVKHVLKVSL++TRYEYYTVGKY+P  DKYVPDNTS DGW
Sbjct: 250 DFYPVPLRGRLGLDPSVTGNWVKHVLKVSLDLTRYEYYTVGKYFPKKDKYVPDNTSVDGW 309

Query: 301 SGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSG 360
           SGLRYDYGNFYASKSFYDP++KRRVLWGWANESDS QDDVSKGWAGIQ IPRTVWLD + 
Sbjct: 310 SGLRYDYGNFYASKSFYDPLRKRRVLWGWANESDSAQDDVSKGWAGIQLIPRTVWLDHNQ 369

Query: 361 KQLLQWPVMELNRLRRKR-----------------------ADVEVIFSFSSLDKAEVFD 420
           KQL+QWPV ELN LR K+                       ADVEVIFSFSSLDKAE FD
Sbjct: 370 KQLIQWPVEELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVIFSFSSLDKAEPFD 429

Query: 421 SSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCS 480
             WVDAQA C QLG  +QGGVGPFGLLTLASENLEEFTPVFFRIFK       HVVLMCS
Sbjct: 430 PRWVDAQAACDQLGLKSQGGVGPFGLLTLASENLEEFTPVFFRIFKA---HHKHVVLMCS 489

Query: 481 DARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTK 540
           DARSSSLK DGLYKP FAG+VDIDLSSKKL LRSLIDHSV+ESFGGGGKTCITSRVYPTK
Sbjct: 490 DARSSSLKEDGLYKPAFAGYVDIDLSSKKLSLRSLIDHSVVESFGGGGKTCITSRVYPTK 549

Query: 541 AVFEAAHLHVFNNGTESITVEYLSAWSMRSAWVN 551
            VF  AHLHVFNNGTE+ITVEYLSAWSM+SA VN
Sbjct: 550 GVFNDAHLHVFNNGTEAITVEYLSAWSMKSARVN 575

BLAST of Cp4.1LG12g04140 vs. NCBI nr
Match: gi|449462946|ref|XP_004149196.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucumis sativus])

HSP 1 Score: 927.5 bits (2396), Expect = 1.0e-266
Identity = 443/574 (77.18%), Postives = 485/574 (84.49%), Query Frame = 1

Query: 1   ILLGFSFYSLFIDNGPVVVQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMND 60
           +LLGFSF+SLFI+NG V V ALHKIYPELQS++   D   IK LHRT FHFQP R+W+ND
Sbjct: 9   LLLGFSFFSLFINNGVVFVDALHKIYPELQSIQ--TDASVIKPLHRTRFHFQPRRNWIND 68

Query: 61  PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWS 120
           PNGPMYFKGIYHLFYQYNP+GAVWGNIVWAHSISRDLINWK LKPA+ PSKPFDINGCWS
Sbjct: 69  PNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKPAIYPSKPFDINGCWS 128

Query: 121 GSATVLPGFKPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSS 180
           GSATVLPG KPVILYTGID QNRQVQNYAIPANL DP+LTEW+KPDNNPIV PG GVN+S
Sbjct: 129 GSATVLPGDKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDNNPIVDPGPGVNAS 188

Query: 181 AFRDPSTAWLSFKGHWKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECP 240
           AFRDP+TAWLS  GHWKT+IGSK ++ G+AYLY+S+DF+ WTK + PLHS  N+GMWECP
Sbjct: 189 AFRDPTTAWLSKSGHWKTVIGSKRKKRGMAYLYRSRDFVKWTKAKHPLHSAANTGMWECP 248

Query: 241 DFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGW 300
           DFYPV   G+ GLD SVTGNWVKHV KVSL++TRYEYYTVGKY+P  DKYVPDNTS DGW
Sbjct: 249 DFYPVPLRGKLGLDPSVTGNWVKHVFKVSLDLTRYEYYTVGKYFPKKDKYVPDNTSVDGW 308

Query: 301 SGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSG 360
           SGLRYDYGNFYASKSFYDP+KKRRVLWGWANESDS QDDVSKGWAGIQ IPRTVWLD + 
Sbjct: 309 SGLRYDYGNFYASKSFYDPLKKRRVLWGWANESDSAQDDVSKGWAGIQLIPRTVWLDHNQ 368

Query: 361 KQLLQWPVMELNRLRRKR-----------------------ADVEVIFSFSSLDKAEVFD 420
           +QL QWPV ELN LR K+                       ADVEV+FSFSSLDKA+ FD
Sbjct: 369 RQLKQWPVKELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVMFSFSSLDKADPFD 428

Query: 421 SSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCS 480
             WVDAQA C QLG++ QGGVGPFGLLTLASENL+EFTPVFFRIFK       HVVLMCS
Sbjct: 429 PRWVDAQAACDQLGNS-QGGVGPFGLLTLASENLDEFTPVFFRIFKA---HHKHVVLMCS 488

Query: 481 DARSSSLK-DGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTK 540
           DARSSSLK +GLYKP FAG+VDIDLS KKL LRSLIDHSV+ESFGGGGKTCITSRVYPTK
Sbjct: 489 DARSSSLKEEGLYKPAFAGYVDIDLSRKKLSLRSLIDHSVVESFGGGGKTCITSRVYPTK 548

Query: 541 AVFEAAHLHVFNNGTESITVEYLSAWSMRSAWVN 551
            VF+ AHLHVFNNGTE+ITVEYL AWSM+SA +N
Sbjct: 549 GVFDDAHLHVFNNGTEAITVEYLRAWSMKSARMN 576

BLAST of Cp4.1LG12g04140 vs. NCBI nr
Match: gi|657989615|ref|XP_008387018.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like isoform X1 [Malus domestica])

HSP 1 Score: 808.5 bits (2087), Expect = 7.1e-231
Identity = 376/567 (66.31%), Postives = 444/567 (78.31%), Query Frame = 1

Query: 10  LFIDNGPVVVQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKG 69
           +F  N    V+ALH +YPE QSL      +++KQ+HRT FHFQP R+W+NDPNGPMYF G
Sbjct: 20  VFAINNITGVEALHNVYPEFQSLA----AVKVKQIHRTAFHFQPPRNWINDPNGPMYFGG 79

Query: 70  IYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGF 129
           +YHLFYQYNPEGAVWGNIVWAHS+S+DLINW++L+PA+ PSKPFD+NGCWSGSAT LPG 
Sbjct: 80  LYHLFYQYNPEGAVWGNIVWAHSVSKDLINWEALEPAIYPSKPFDVNGCWSGSATXLPGN 139

Query: 130 KPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAW 189
           KP++LYTG+DSQNRQVQNYAIP N  DP+L +W+KPDNNP+V+P TG+N++AFRDP+TAW
Sbjct: 140 KPIMLYTGVDSQNRQVQNYAIPKNASDPYLRKWIKPDNNPLVIPDTGINATAFRDPTTAW 199

Query: 190 LSFKGHWKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFG 249
               GHWK ++G K +  G+A+LY+SKDF++W K   PLHS P +GMWECPDFYPV   G
Sbjct: 200 WG-NGHWKMLVGGKRKHRGMAHLYRSKDFVHWVKAHHPLHSAPQTGMWECPDFYPVPLVG 259

Query: 250 RFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGN 309
           + GLDTS  G  VKHVLKVSL+ TRYEYYTVGKY+P  D YVPD    DGWSGLRYDYGN
Sbjct: 260 KLGLDTSKVGGDVKHVLKVSLDETRYEYYTVGKYFPEKDTYVPDKELVDGWSGLRYDYGN 319

Query: 310 FYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVM 369
           FYASK+F+DP KKRR+LWGWANESD+  +DV+KGWAGIQ IPR VWL P GKQLLQWPV+
Sbjct: 320 FYASKTFFDPAKKRRILWGWANESDTASNDVAKGWAGIQTIPRDVWLSPDGKQLLQWPVV 379

Query: 370 ELNRLRRKR-----------------------ADVEVIFSFSSLDKAEVFDSSW--VDAQ 429
           EL  LR K+                       ADVEVIFS  SL KAE F+ SW  +DAQ
Sbjct: 380 ELETLRGKKVGINNQILQQGSHVEVKGLTAAQADVEVIFSLPSLAKAEEFNPSWSNLDAQ 439

Query: 430 AVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSL 489
            +C + GS  QGG+GPFGLLTLASENLEEFTPVFFR+FK    +  HVVLMCS A SSSL
Sbjct: 440 TLCGKKGSKIQGGIGPFGLLTLASENLEEFTPVFFRVFKA---KGRHVVLMCSXATSSSL 499

Query: 490 KDGLYKPTFAGFVDIDLS-SKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAH 549
           +D LY+P+FAGFVD+DLS SKKL LRSLIDHSV+ES+G GGKTCITSRVYPT AV   AH
Sbjct: 500 QDNLYRPSFAGFVDVDLSHSKKLSLRSLIDHSVVESYGAGGKTCITSRVYPTLAVNNGAH 559

Query: 550 LHVFNNGTESITVEYLSAWSMRSAWVN 551
           L+VFNNGT+ +T E L+AWSM +  +N
Sbjct: 560 LYVFNNGTQPVTAESLNAWSMNAPTMN 578

BLAST of Cp4.1LG12g04140 vs. NCBI nr
Match: gi|702249497|ref|XP_010059629.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 3-like [Eucalyptus grandis])

HSP 1 Score: 807.7 bits (2085), Expect = 1.2e-230
Identity = 371/555 (66.85%), Postives = 441/555 (79.46%), Query Frame = 1

Query: 19  VQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKGIYHLFYQYN 78
           V+A HK+YPE QSL      + +K  HRTG+HFQP +HW+NDPNGPM++KG YHLFYQYN
Sbjct: 33  VEARHKVYPEYQSLP----AVSVKYAHRTGYHFQPPKHWINDPNGPMHYKGWYHLFYQYN 92

Query: 79  PEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGFKPVILYTGI 138
           P+GAVWGNIVWAHS+S+DL+NW++L PA+ P+KPFDINGCWSGSATVLPG +P+ILYTGI
Sbjct: 93  PKGAVWGNIVWAHSVSKDLVNWRALPPAIYPTKPFDINGCWSGSATVLPGNRPIILYTGI 152

Query: 139 DSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAWLSFKGHWKT 198
           D++N QVQNYA+PAN  DP+L EW KPDNNPIV PG GVN+SAFRDP+TAW    GHWK 
Sbjct: 153 DTKNHQVQNYAVPANASDPYLREWTKPDNNPIVDPGPGVNASAFRDPTTAWFE-DGHWKI 212

Query: 199 IIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFGRFGLDTSVT 258
           ++GS+ +  G+AYLY+S+DF++WTK + PLHS P +GMWECPDF+PVS     GLDTS  
Sbjct: 213 LVGSRRKHRGMAYLYRSRDFIHWTKAKHPLHSAPKTGMWECPDFFPVSKNNANGLDTSAL 272

Query: 259 GNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYD 318
           G  VKHVLKVSL++TRYEYYT+G Y+P  D+Y+PDNTSADGWSGLRYDYGNFYASK+F+D
Sbjct: 273 GQGVKHVLKVSLDLTRYEYYTIGTYHPDKDRYMPDNTSADGWSGLRYDYGNFYASKTFFD 332

Query: 319 PMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVMELNRLRRK- 378
           P+K RR+LWGWANESD+VQ DV KGWAGIQ IPR +WLD  GKQLLQWPV EL  LR + 
Sbjct: 333 PVKNRRLLWGWANESDAVQSDVQKGWAGIQTIPRKIWLDSYGKQLLQWPVEELETLRGQN 392

Query: 379 ----------------------RADVEVIFSFSSLDKAEVFDSSWVDAQAVCSQLGSTAQ 438
                                 +ADV+V+FSFSSLD AE FDSSW++AQ +C   GS   
Sbjct: 393 VHMSSVKLNNGEHVEIKGITAAQADVDVVFSFSSLDGAEQFDSSWLNAQDLCGLKGSGVP 452

Query: 439 GGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSLKDGLYKPTFAG 498
           G VGPFGLLTLASE+LEEFTPVFFRIFK + ++  H VLMCSDARSSSLK G+YKP+FAG
Sbjct: 453 GNVGPFGLLTLASEHLEEFTPVFFRIFKAD-DQNKHKVLMCSDARSSSLKSGIYKPSFAG 512

Query: 499 FVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAHLHVFNNGTESIT 551
           FVD+DLS  K+ LRSLIDHSV+ESFG GGKTCITSRVYPT A+   A L  FNNGT++IT
Sbjct: 513 FVDVDLSDGKISLRSLIDHSVVESFGAGGKTCITSRVYPTLALLGNARLFTFNNGTQTIT 572

BLAST of Cp4.1LG12g04140 vs. NCBI nr
Match: gi|694394216|ref|XP_009372510.1| (PREDICTED: beta-fructofuranosidase, insoluble isoenzyme 1-like [Pyrus x bretschneideri])

HSP 1 Score: 807.4 bits (2084), Expect = 1.6e-230
Identity = 375/567 (66.14%), Postives = 444/567 (78.31%), Query Frame = 1

Query: 10  LFIDNGPVVVQALHKIYPELQSLEKGNDTIEIKQLHRTGFHFQPHRHWMNDPNGPMYFKG 69
           +F  N    V+ALH +YPE QSL      +++KQ+HRT FHFQP R+W+NDPNGPMYF G
Sbjct: 21  VFAINNITGVEALHNVYPEFQSLA----AVKVKQVHRTAFHFQPPRNWINDPNGPMYFGG 80

Query: 70  IYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKPFDINGCWSGSATVLPGF 129
           +YHLFYQYNPEGAVWGNIVWAHS+S+DLINW++L+PA+ PSKPFD+NGCWSGSATVLPG 
Sbjct: 81  LYHLFYQYNPEGAVWGNIVWAHSVSKDLINWEALEPAIHPSKPFDVNGCWSGSATVLPGN 140

Query: 130 KPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVVPGTGVNSSAFRDPSTAW 189
           KP+ILYTGIDSQNRQVQNYAIP N  DP+L +W+KPDNNP+V+P  G+N++AFRDP+TAW
Sbjct: 141 KPIILYTGIDSQNRQVQNYAIPENASDPYLRKWIKPDNNPLVIPDLGINATAFRDPTTAW 200

Query: 190 LSFKGHWKTIIGSKSEQTGIAYLYKSKDFLNWTKVEQPLHSVPNSGMWECPDFYPVSHFG 249
               GHWK ++G K +  G+AYLY+SKDF++W K   PLHS P +GMWECPDFYPV   G
Sbjct: 201 WG-NGHWKMLVGGKRKHRGMAYLYRSKDFVHWVKAHHPLHSAPQTGMWECPDFYPVPLVG 260

Query: 250 RFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGN 309
           + GLDTS  G  VKHVLKVSL+ TRYEYYTVG Y+P  D+YVPD    DGWSGLRYDYGN
Sbjct: 261 KLGLDTSKVGRDVKHVLKVSLDETRYEYYTVGTYFPEKDRYVPDKELVDGWSGLRYDYGN 320

Query: 310 FYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPSGKQLLQWPVM 369
           FYASK+F+DP KKRR+LWGWANESD+  +D +KGWAGIQ IPR VWL P GKQLL+WPV+
Sbjct: 321 FYASKTFFDPAKKRRILWGWANESDTASNDAAKGWAGIQTIPRDVWLSPDGKQLLKWPVV 380

Query: 370 ELNRLRRKR-----------------------ADVEVIFSFSSLDKAEVFDSSW--VDAQ 429
           EL  LR K+                       ADVEVIF+  SL KAE F+ SW  +DAQ
Sbjct: 381 ELETLRGKKVGINKQILKQGDHVEVKGITAAQADVEVIFTLPSLAKAEEFNPSWSNLDAQ 440

Query: 430 AVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNETNHVVLMCSDARSSSL 489
            +C + GS  QGG+GPFGLLTLAS+NLEEFTPVFFR+FK    +  HVVLMCSDA SSSL
Sbjct: 441 TLCGKKGSKIQGGIGPFGLLTLASKNLEEFTPVFFRVFKA---KGRHVVLMCSDATSSSL 500

Query: 490 KDGLYKPTFAGFVDIDLS-SKKLPLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFEAAH 549
           +D LY+P+FAGFVD+DLS SKKL LRSLIDHSV+ES+G GGKTCITSRVYPT AV   AH
Sbjct: 501 QDNLYRPSFAGFVDVDLSHSKKLSLRSLIDHSVVESYGAGGKTCITSRVYPTLAVNNGAH 560

Query: 550 LHVFNNGTESITVEYLSAWSMRSAWVN 551
           L+VFNNGT+ +T E L+AWSM +  +N
Sbjct: 561 LYVFNNGTQPVTAESLNAWSMNAPTMN 579