Cp4.1LG12g03650 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG12g03650 vs. Swiss-Prot
Match: PUB33_ARATH (U-box domain-containing protein 33 OS=Arabidopsis thaliana GN=PUB33 PE=2 SV=2)

HSP 1 Score: 711.1 bits (1834), Expect = 1.4e-203
Identity = 381/809 (47.10%), Postives = 539/809 (66.63%), Query Frame = 1

Query: 7   DDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEV 66
           D+ +++AV   V + +  L++A+++ GG++IC++HVH+P+++IP +G +F   ++KE+EV
Sbjct: 44  DEKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEV 103

Query: 67  NAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAA 126
             +RE+ER+  + ILD +L +C Q GVRAEK+++E + I+ GIV+L+S  GIR+L+MGAA
Sbjct: 104 RVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVMGAA 163

Query: 127 ADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPPSPQ- 186
           AD++YSR+MT ++S+KA++V  +AP  CQI F C G LI TREA   +   +   P P  
Sbjct: 164 ADRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPRPSI 223

Query: 187 SQNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPSSSSGC 246
           S +     +   ES   + SR+QS        S+ +LVSN S T        S   S G 
Sbjct: 224 SASDLLQTFSTPESEHQHISRVQSTD------SVQQLVSNGSSTEQ------SGRVSDGS 283

Query: 247 LDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQQDESADDILYIQLEKSITEAA 306
           L+      E D   V  S+ ++        S  H S     +  DD   +++ K+ +EA 
Sbjct: 284 LNTDEEERESDGSEVTGSATVM--------SSGHSSPSSFPDGVDDSFNVKIRKATSEAH 343

Query: 307 NAKREAFREAVKRAQAENELGNAIFRARE-----------RKDVEEALSKEREELENVKN 366
           ++K+EAF E ++R +AE    +AI RA++           RKD E A++KE+E    +KN
Sbjct: 344 SSKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEELKRRKDTEIAVAKEKERFITIKN 403

Query: 367 QVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDVLLKQRDE 426
           +   +MEELQ +  +   LE+QIA SD  +++L QK+  A++LL   +++R+ L  +RD 
Sbjct: 404 EQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIAVKLLQKLRDEREELQTERDR 463

Query: 427 ALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYN 486
           AL+E +++R+   E  T    Q F++FSFSEI EAT  FD +LKIGEG +GS+Y GLL +
Sbjct: 464 ALREAEELRSH-AETSTLQLPQYFTDFSFSEIEEATNHFDSTLKIGEGGYGSIYVGLLRH 523

Query: 487 TEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDR 546
           T+V IKM   +S Q PVE+Q+EVDVLS +RHPNI TLIG CPE   LVY+Y P GSLEDR
Sbjct: 524 TQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLIGACPEGWSLVYEYLPGGSLEDR 583

Query: 547 LACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGF 606
           L CKDNS PLSW+ R+RIA+E+C+AL+F+HSNK   ++HGDLKP N+LLD+N V KL+ F
Sbjct: 584 LTCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSLVHGDLKPANILLDSNLVSKLSDF 643

Query: 607 GSCHFLPHDEKLSYNENIP---AKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYLRM 666
           G+C  L  +   S   ++    A    +++    LT K DV+SFG++LL LLTG+  LR+
Sbjct: 644 GTCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTPKSDVYSFGIILLRLLTGRPALRI 703

Query: 667 KEDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEP 726
             +V++A++   L ++LDP AGDWPFVQAEQLA+LALRCC   S  RPDL ++VWRVLEP
Sbjct: 704 SNEVKYALDNGTLNDLLDPLAGDWPFVQAEQLARLALRCCETVSENRPDLGTEVWRVLEP 763

Query: 727 MKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH 786
           M+AS GGS S  L   + ++  PPYF+CPIFQEVM+DPHVAADGFTYEAEA+R WLDS H
Sbjct: 764 MRASSGGSSSFHLGRNEHRI-APPYFICPIFQEVMQDPHVAADGFTYEAEAIRAWLDSEH 823

Query: 787 NTSPMTNLRLEHQNLVPNRALRSVIQEWL 801
           +TSPMTN++L H +L+ N ALRS IQEWL
Sbjct: 824 DTSPMTNVKLSHTSLIANHALRSAIQEWL 830

BLAST of Cp4.1LG12g03650 vs. Swiss-Prot
Match: PUB32_ARATH (U-box domain-containing protein 32 OS=Arabidopsis thaliana GN=PUB32 PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 2.4e-142
Identity = 300/813 (36.90%), Postives = 475/813 (58.43%), Query Frame = 1

Query: 7   DDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEV 66
           D+ +++AV  DV   +  +++A ++  G++IC+L+VH  A+   +   + +  S K+ +V
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDV 73

Query: 67  NAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAA 126
                 E+   +++++ +L L  +  ++ +KL +    I++ IVEL++ H I+ L+MGAA
Sbjct: 74  KVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAA 133

Query: 127 ADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPPSPQS 186
           +DK+YS KMT ++SKKA++V  KAP  C I F+C G LI TR +     +    PP  Q 
Sbjct: 134 SDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIFTRASNDDSNNRQTMPPLVQL 193

Query: 187 QNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGT--SSPFERSYSPSSSSG 246
            + N          +   S     S +  RL   + +    G   +   ER      +  
Sbjct: 194 DSDN----------ETRKSEKLESSYMRRRLRYWRSLLEQDGEKDTGQLEREKVEPRAPP 253

Query: 247 CLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQQDESADDIL--YIQLEKSIT 306
              + SS +  +  G    SP L+     +S  L+ S  ++ E   D+     + +K++ 
Sbjct: 254 LFSSGSSSSFGEPVGPEPVSPELV-----DSDTLNTSNVEEKEREGDVARKVHRYDKAMH 313

Query: 307 EAANAKREAFREAVKRAQAENELGNAIFRAR-----------ERKDVEEALSKEREELEN 366
           +   + R  + EA K+ + +     A+ +A+           +RK +EE L KE+ E++ 
Sbjct: 314 DIGQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKM 373

Query: 367 VKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDVLLKQ 426
           V  Q N  M+ELQ+ + R L+LE+Q+     + KE  +K  +A+ELL +++  RD +   
Sbjct: 374 VIEQNNGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFRQKRDEIRID 433

Query: 427 RDEALKEVDDIRTRQVEART-KHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKG 486
            + A+KEV+ +R R V+  T + +     ++SF EI EAT +FDPS K+GEG +GS+YKG
Sbjct: 434 HENAVKEVNALR-RLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKG 493

Query: 487 LLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGS 546
            L + +V +KM  S+   N  EF+R V++LS +RHPN+ TL+G CPE+  L+Y Y PNGS
Sbjct: 494 NLQHLQVAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGACPESRSLIYQYIPNGS 553

Query: 547 LEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPK 606
           LED  + ++N   LSW++RIRIASE+CSAL+F+HSN  C IIHG+LKP  +LLD+N V K
Sbjct: 554 LEDCFSSENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTK 613

Query: 607 LAGFGSCHFLPHDEKLSYNENIPAKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYLR 666
           +  +G    +P D     + ++   Y V       +T + D+++FG++LL LLT +    
Sbjct: 614 INDYGISQLIPIDGLDKSDPHVDPHYFVSRE----MTLESDIYAFGIILLQLLTRRPVSG 673

Query: 667 MKEDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLE 726
           +  DV+ A+E   +  VLD  AGDWP  + ++LA +A+RCC +N M RPDL + V R ++
Sbjct: 674 ILRDVKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDL-AVVLRFID 733

Query: 727 PMKA-SCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDS 786
            MKA     S + S +  ++  +PP ++LCPIFQEVM+DP +AADGFTYEAEA+R WL +
Sbjct: 734 RMKAPEVPSSETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLAN 793

Query: 787 GHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQ 803
           GH+TSPMTNL++E  NL+PN AL   IQ+W +Q
Sbjct: 794 GHDTSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of Cp4.1LG12g03650 vs. Swiss-Prot
Match: PUB57_ORYSJ (U-box domain-containing protein 57 OS=Oryza sativa subsp. japonica GN=PUB57 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.7e-87
Identity = 173/431 (40.14%), Postives = 248/431 (57.54%), Query Frame = 1

Query: 381 DQMVKELEQKILSAIELLHNYKNDRDVLLKQRDEALKEVDDIR------TRQVEARTKHT 440
           D+   E  +K+    E +   K+  D  L Q+++A+   D ++       +QV      +
Sbjct: 81  DRKTYEEVRKVHILEEEIVTLKHQADTYLVQKEKAVTAYDQLKHERDNAVQQVNELRDQS 140

Query: 441 AQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQ 500
             +  +FS  ++ +AT  F  + ++G+  +G  YKG+++N +V IK+  S  L     FQ
Sbjct: 141 THIILDFSRKDMEQATEHFKNAREVGDTEYGHTYKGMIHNMKVLIKLSSSQKL-----FQ 200

Query: 501 REVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIAS 560
           +EV +L   RHPNI T IGVC E   LVY++ PNG+LEDR+ C +NS+PLSW  R +I  
Sbjct: 201 QEVSILRQWRHPNIITFIGVCSEVSALVYEWLPNGNLEDRIICTNNSAPLSWYNRTQIIG 260

Query: 561 ELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENIPA 620
           E+C AL+F+HSNK   ++HGDL+P N+L+DANY  K+  FG  +          N     
Sbjct: 261 EICCALLFLHSNKSTALVHGDLRPCNILIDANYRSKICNFGMSNLFLQLGTFPPNLTARL 320

Query: 621 KY-GVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAVEERKLKNVLDPRAG 680
            Y   + N    LTT  DV+S G+++L LLTG   L + E V  A+    L  ++D  AG
Sbjct: 321 PYMDPEFNTTGELTTLSDVYSLGVIILRLLTGMPPLTLSEKVAEALGSDSLHLLIDKSAG 380

Query: 681 DWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQP 740
           DWP+++A+QLA + L C       RPDL+++VW V+EP+      +    L         
Sbjct: 381 DWPYIEAKQLALIGLSCTGMTRKKRPDLLNEVWIVIEPLTRKPPAATWPYLQSASGDSSV 440

Query: 741 PPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALR 800
           P  F+CPI  E+M+DP VA+DGFTYEAEA+R W D G + SPMTNL L + NLVPNR LR
Sbjct: 441 PAAFICPISMEIMKDPQVASDGFTYEAEAIRCWFDRGISRSPMTNLALPNLNLVPNRVLR 500

Query: 801 SVIQEWLHQQQ 805
           S I  +L QQQ
Sbjct: 501 SFIHGYLQQQQ 506

BLAST of Cp4.1LG12g03650 vs. Swiss-Prot
Match: PUB35_ARATH (U-box domain-containing protein 35 OS=Arabidopsis thaliana GN=PUB35 PE=1 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 3.4e-80
Identity = 233/794 (29.35%), Postives = 373/794 (46.98%), Query Frame = 1

Query: 39  ILHVHEPAKLIPF-LGTQFLASSMKEKEVNAYRERERQDANKILDGFLFLCHQAGVRAEK 98
           +LH+H     +P  +G     S +++  V AYR+     + ++L  +  L  +  V  E 
Sbjct: 53  LLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEV 112

Query: 99  LYVESDKIKKGIVELVSLHGIRRLIMGAAADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQ 158
           L +ESD +   I E V+   I R+++G ++  ++SRK        A+      P FC + 
Sbjct: 113 LVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSFFSRKADICSVISALM-----PNFCTVY 172

Query: 159 FVCNGQLIRTREACPQEAHVDIPPPSPQSQNVNGACWRPVESGQ-----FNGSRIQSPSI 218
            V  G+L   R +   + +  I     +  N +     P           + S+ +  S+
Sbjct: 173 VVSKGKLSCVRPS-DSDGNATIREDGSERTNSSSGSSGPTSDSSDVMSSAHDSQSRPLSL 232

Query: 219 VLERLSISKLVSNDSGTSSPFERSYSPSSSSGC--LDAASSRTEEDEYGVGLSSPLLLKD 278
            + R+     ++  +  S P E S   S  + C  LDA  +R   D   +  SS      
Sbjct: 233 PVRRMQHFPAIAGQA--SVPMETSSVGSDETRCMSLDAEEAR---DVSSINRSSTDTTSR 292

Query: 279 VAPNSSPLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELGNAIF 338
             P              S+ +  Y       + +          A ++A   ++  +   
Sbjct: 293 WTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTHSRASQQASNMSDALSEQS 352

Query: 339 RARERKDVEEALSKEREELENVKNQVN-KMMEELQVSRNRG----LELENQIAGSDQMVK 398
               + ++   + K R EL +V+        E    SR  G      LE  I   +  +K
Sbjct: 353 YTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGELNQRRLEEAIKLEELKLK 412

Query: 399 ELEQKILSAIELLHNYKNDRDV----------LLKQRDEALKEVDDIRTRQVEARTKHTA 458
           E E + L+  E  +  K  RD           + ++R+   K   D + ++    T  + 
Sbjct: 413 EYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKSARDTKEKEKLEGTLGSP 472

Query: 459 QV-FSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQ 518
           Q+ +  F++ EI  AT  F   LKIG G +G++YK  L++T   +K+  S   Q   +FQ
Sbjct: 473 QLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQ 532

Query: 519 REVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIAS 578
           +E+++LS +RHP++  L+G CPE   LVY+Y  NGSLEDRL   +NS PL W  R RIA 
Sbjct: 533 QELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAW 592

Query: 579 ELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFL---PHDEKLS-YNE 638
           E+ +AL+F+H +K   IIH DLKP N+LLD N+V K+   G    +   P   K + Y +
Sbjct: 593 EVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQ 652

Query: 639 NIPAKYGVKSNHEFP----LTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAVEER-KLK 698
             P       + E+     +++K D++SFGM+LL LLT K  + +   V+ A++   +  
Sbjct: 653 TSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAIALTHFVESAMDSNDEFL 712

Query: 699 NVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLS 758
            +LD +AG+WP  +  +LA LAL C       RPDL   +   LE +K     +     S
Sbjct: 713 KILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPALENLKKV---AEKARNS 772

Query: 759 FGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQN 800
           F  +  QPP +F+CP+ ++VM +P VAADG+TY+  A+  WL   HNTSPMT+  L  +N
Sbjct: 773 FSGVSTQPPTHFICPLLKDVMNEPCVAADGYTYDRHAIEEWLKE-HNTSPMTDSPLHSKN 831

BLAST of Cp4.1LG12g03650 vs. Swiss-Prot
Match: PUB53_ARATH (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana GN=PUB53 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 1.0e-73
Identity = 197/628 (31.37%), Postives = 318/628 (50.64%), Query Frame = 1

Query: 225 NDSGTSSPFERSYSPSSSSGCLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQ 284
           ND+   S FER+ S  SS                G G +S ++   +  N   L     Q
Sbjct: 214 NDTSIESGFERTSSSCSS----------------GSGANSDVMSNALKSNPHTLSNKRMQ 273

Query: 285 QDESADDILYIQLEKSITEAANAKR---EAFREAVKRAQAENELG---NAIFRA-RERKD 344
              +    + + +E S TE+   K+   +A  EA KR+  E       N  FR   ERKD
Sbjct: 274 NLPTIVRGVSVPMETSSTESDETKKRSSDAAEEASKRSSPETSRSVSWNPQFRDFDERKD 333

Query: 345 VEEALSKERE-------------ELENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMV 404
              ++S   E             + ++  N+++K+  EL+ +       + +   + + +
Sbjct: 334 AMSSMSSNFEYGNVVTPLGHYFTDNQDTLNEISKLRAELRHAHEMYAVAQVETLDASRKL 393

Query: 405 KELEQKILSAIELLHNYKN-DRDVLLKQRDEALKEVDDIRTRQVEARTKHTAQV------ 464
            EL+ + L+ +E  H  K   +    K   +  +E +  + R+ E +  H A+       
Sbjct: 394 NELKFEELTLLE--HETKGIAKKETEKFEQKRREEREAAQRREAEMKATHEAKEKEKLEE 453

Query: 465 ---------FSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQ 524
                    + EF++ EI  AT  F   LKIG G +G +YK  L++T   +K+  S    
Sbjct: 454 SSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLHSAESS 513

Query: 525 NPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKT 584
              +F +E+++LS +RHP++  L+G CP+   LVY+Y  NGSLEDRL   ++S P+ W  
Sbjct: 514 LSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQPIPWFV 573

Query: 585 RIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLS- 644
           R+RIA E+ SAL+F+H +K   IIH DLKP N+LL+ N+V K+   G    +   + LS 
Sbjct: 574 RLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAADPLST 633

Query: 645 ----YNENIPAKYGVKSNHEFP----LTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAV 704
               Y +  P       + E+     ++ K DV++FGM++L LLTG+  + +   V+ A+
Sbjct: 634 KFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTYTVETAM 693

Query: 705 E---ERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASC 764
           E   + +L  +LD +AG+WP  +  QLA LAL+C    S  RPDL   +  VLE +K   
Sbjct: 694 ENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLESLKKVA 753

Query: 765 GGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPM 805
             + +   S      QPP +F CP+ ++VM++P +AADG+TY+  A+  W+++ H TSP+
Sbjct: 754 DKARN---SLSAAPSQPPSHFFCPLLKDVMKEPCIAADGYTYDRRAIEEWMEN-HRTSPV 813

BLAST of Cp4.1LG12g03650 vs. TrEMBL
Match: A0A0A0KD01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G004520 PE=4 SV=1)

HSP 1 Score: 1294.6 bits (3349), Expect = 0.0e+00
Identity = 650/815 (79.75%), Postives = 717/815 (87.98%), Query Frame = 1

Query: 1   MAGSCGDDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASS 60
           MAGS G+D+VY+AVGND+NEC+LNLVYAIKH GGRRICILHVHEPAK IP LGT+FLASS
Sbjct: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60

Query: 61  MKEKEVNAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRR 120
           MKE+EVNAYRE ERQDA+KILD FL LCHQAG RAEKLY ESDKIKKGIVELVSLHGIR 
Sbjct: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120

Query: 121 LIMGAAADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIP 180
           L+MGAAADKYYSRKMTKIRS+KAMYVHL A A CQIQFVC GQLIR REACPQE H DI 
Sbjct: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180

Query: 181 PPSPQSQNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPS 240
           P SPQSQN+NG  WR  + G FNG    SP+IV+ERL+IS+ +SN SG  SPFERSY+ S
Sbjct: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240

Query: 241 SSSGCLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQQDESADDILYIQLEKS 300
            SSG +D ASSRTEED YG+GL+SPLLL + A  +SP H SGFQQD SADD LYIQLEK+
Sbjct: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300

Query: 301 ITEAANAKREAFREAVKRAQAENELGNAIFRA-----------RERKDVEEALSKEREEL 360
           I +AANA+REAFREA+KRA+AE EL  AI  A           R RKD EE LSKEREEL
Sbjct: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360

Query: 361 ENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDVLL 420
           ++VKNQVN+MM+ELQ++RN GL+LENQIA SD+MVKELEQKILSAIELLHNYKNDRD LL
Sbjct: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420

Query: 421 KQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYK 480
           KQRDEALKE+DDIRTRQVEA ++H+AQ+ SEFSFSEI EATRKFDPSLKI    +GSMYK
Sbjct: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480

Query: 481 GLLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNG 540
           GLLYNTEV IKM  SH+LQNPV+FQREVD LS LRHPNIATLIGVCPEAC+LVYDYFPNG
Sbjct: 481 GLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNG 540

Query: 541 SLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVP 600
           +LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHSNKICK +HGD+KP N+LLDANYVP
Sbjct: 541 NLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVP 600

Query: 601 KLAGFGSCHFLPHDEKLSYNENIPAKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYL 660
           KLAGFG+CHFL HDEKLSYNEN+   Y  K NHEFPLTT+LD++SFG VLLSLLTGKSYL
Sbjct: 601 KLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYL 660

Query: 661 RMKEDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVL 720
           RMKEDVQ A+++RKLK+VLDPRAGDWPFVQAEQLAQLALRCC+ +SMYRPDLVSDVWRVL
Sbjct: 661 RMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVL 720

Query: 721 EPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 805
           GH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 813

BLAST of Cp4.1LG12g03650 vs. TrEMBL
Match: M5X7L4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001267mg PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 9.7e-228
Identity = 444/856 (51.87%), Postives = 574/856 (67.06%), Query Frame = 1

Query: 7   DDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEV 66
           +D++Y+AV  DV + +  LV+A+ + GG++IC+ HVH+P++ IP +G  F ASS+K++EV
Sbjct: 17  EDMIYVAVAKDVKDSKSTLVWAVHNSGGKKICLAHVHQPSQKIPCMGGWFPASSLKDEEV 76

Query: 67  NAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAA 126
            AYRE ERQ+ NKIL+ +  +C Q GVRAEKL++E D I+KGIVEL+S HGIR+L+MGAA
Sbjct: 77  RAYREIERQNMNKILEDYFRICRQMGVRAEKLHIEMDCIEKGIVELISQHGIRKLVMGAA 136

Query: 127 ADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIP--PPSP 186
           ADKY+SRKM  ++SKKA+YV  +AP  C IQF+C G LI TRE        D+P   PSP
Sbjct: 137 ADKYHSRKMMDLKSKKAIYVRQQAPVSCHIQFICKGHLIYTREGNSDGVDTDVPLLQPSP 196

Query: 187 QSQNVNGA----CWRPVESGQFNGSRIQSPSIVLERL-----------SISKLVSNDS-- 246
            S              V  GQ N +++ +P+  L R            SI++  S+D   
Sbjct: 197 NSDPEQSPHHFRSRSAVTLGQNNRAKLTNPAQDLYRRVRSANMEKYGGSITEATSSDGTE 256

Query: 247 GTSSP--FERSYSP-----------SSSSGCLDAASS-------RTEEDEYGVGLSSPLL 306
           G S+P  FE   SP           S  S C  A          R E  E G   S  L 
Sbjct: 257 GLSTPSRFEAGGSPDDWDRVSRRSVSGYSSCSSALGDLALVQYDRIEGSENGSTESHALS 316

Query: 307 -LKDVAPNSSPLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELG 366
             K++  +S P  L G     + DD LY  LE+++ EA NAKREAFRE ++R +AE +  
Sbjct: 317 HFKELNHSSPPSVLDG-----NIDDSLYDHLEQAMAEAENAKREAFREGIRRGKAEKDAI 376

Query: 367 NAIFRA-----------RERKDVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELEN 426
           +AI RA           R+RK++EEAL++EREELE +K Q +++MEEL+ + +    LE+
Sbjct: 377 DAIRRAKASELLYNEELRQRKEIEEALAREREELEKMKKQRDEVMEELRAALDHKSLLES 436

Query: 427 QIAGSDQMVKELEQKILSAIELLHNYKNDRDVLLKQRDEALKEVDDIRTRQVEARTKHTA 486
           QIA SDQM   LEQKI+SA+ELL NYK +RD L  +RD AL+E +++R +Q EA + H  
Sbjct: 437 QIAESDQMAVNLEQKIISAVELLQNYKKERDELHVERDNALREAEELRRKQGEASSSHLP 496

Query: 487 QVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQR 546
           Q F+EFSF+EI EATR FDPSLKIGEG +GS++KG L +T+V IK+  +HS+Q P EFQ+
Sbjct: 497 QFFTEFSFTEIEEATRNFDPSLKIGEGGYGSIFKGSLRHTQVAIKLLHAHSMQGPSEFQQ 556

Query: 547 EVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASE 606
           EVDVLS LRH N+ TLIG CPE+  L+Y+Y  NGSLEDRL+CKDN+ PLSW+TRIRIA+E
Sbjct: 557 EVDVLSKLRHSNLVTLIGACPESWTLIYEYLSNGSLEDRLSCKDNTPPLSWQTRIRIATE 616

Query: 607 LCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENIPAK 666
           LCS LIF+HS+K   I+HGDLKP N+LLD N+V KL+ FG    L   E  S N  +  +
Sbjct: 617 LCSVLIFLHSSKPHGIVHGDLKPANILLDDNFVSKLSDFGISRLLSRGEGSSNNTTLYCR 676

Query: 667 YGVKSNHEF---------PLTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAVEERKLKN 726
              K    +          LT K DV+SFG++LL LLTG+  L + ++VQ+A++  KL+ 
Sbjct: 677 TDPKGTFAYIDPEFLSSGELTPKSDVYSFGIILLRLLTGRPALGITKEVQYALDSGKLET 736

Query: 727 VLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSF 786
           +LDP AGDWPFVQAEQLA LA+RCC  +   R DLVSDVWRVL+PM+ SCG S S  L  
Sbjct: 737 LLDPLAGDWPFVQAEQLACLAMRCCEMSRKRRADLVSDVWRVLDPMRVSCGCSSSFRLGT 796

Query: 787 GDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNL 803
            +   QPP YF+CPIFQEVM+DPHVAADGFTYEAEALRGWLDSGH+TSPMTNL+LEH+NL
Sbjct: 797 EE-HFQPPSYFICPIFQEVMQDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLKLEHKNL 856

BLAST of Cp4.1LG12g03650 vs. TrEMBL
Match: B9GQ99_POPTR (U-box domain-containing family protein OS=Populus trichocarpa GN=POPTR_0002s16150g PE=3 SV=2)

HSP 1 Score: 797.0 bits (2057), Expect = 2.2e-227
Identity = 435/816 (53.31%), Postives = 566/816 (69.36%), Query Frame = 1

Query: 7   DDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEV 66
           +D +++AVG  V EC+L L++A+++ GG+RICI+HV +P+++IP +GT+F AS++KE+EV
Sbjct: 44  EDKIFVAVGKSVKECKLMLLWALQNSGGKRICIIHVLQPSQMIPLMGTKFRASALKEQEV 103

Query: 67  NAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAA 126
            AYRE ERQ  +K+LD +L +C + GVRAEKLYVE + I+KGI+EL+S HGI++L+MGAA
Sbjct: 104 RAYREVERQAMHKMLDEYLSICRKMGVRAEKLYVEMESIEKGILELISHHGIKKLVMGAA 163

Query: 127 ADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPPSPQS 186
           ADK +S+ M  I+SKKA+ V L+APA C I F+C G LI TRE        D+   S Q+
Sbjct: 164 ADKRHSKNMMDIKSKKAISVCLQAPASCHIWFICKGHLIHTREGALDGTGTDVGSSSQQT 223

Query: 187 QNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPSSSSGCL 246
                      E+GQ   S ++S SI L +    KL +N +       RS + +   G L
Sbjct: 224 SP-------HTEAGQL--SHMRSQSIALGQNHFVKL-TNPAQDLVRRVRSMNVNGRGGRL 283

Query: 247 DAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQQDESADDILYIQLEKSITEAAN 306
              +S         G S+P    D   +S          D S +D LY QLEK++++A N
Sbjct: 284 STPASPDG------GPSTPSSRSDADGSSDEY------DDGSTEDPLYDQLEKAMSDAEN 343

Query: 307 AKREAFREAVKRAQAENELGNAIFRA-----------RERKDVEEALSKEREELENVKNQ 366
           ++REAF EAV+RA+AE     A  +A           + RK+VEE L+KE+EELE +  +
Sbjct: 344 SRREAFEEAVRRAKAEKYAFEATRKAKASENLYTEESKRRKEVEEELAKEKEELERINRE 403

Query: 367 VNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDVLLKQRDEA 426
            +++MEEL+++ ++   LE QI  SDQMVKELEQKI+SA+ LL NYK ++D L K+RD A
Sbjct: 404 CDEVMEELRIAEDQKSLLEKQIKESDQMVKELEQKIISAVGLLQNYKKEQDELQKERDNA 463

Query: 427 LKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYNT 486
           LKEV+++R  Q EA   H +Q FSEFS SEI EAT+ FDPSLKIGEG +GS+YKG+L  T
Sbjct: 464 LKEVEELRRSQTEASGTHMSQFFSEFSLSEIEEATQHFDPSLKIGEGGYGSIYKGVLRQT 523

Query: 487 EVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRL 546
           +V +KM  S+SLQ P EFQ+EVDVLS +RHPN+ TLIG CPEA  L+Y+Y PNGSLEDRL
Sbjct: 524 QVAVKMLHSNSLQGPAEFQQEVDVLSKMRHPNLITLIGACPEAWTLIYEYLPNGSLEDRL 583

Query: 547 ACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFG 606
           +C+DNS PLSW+TRIRIA+ELCS LIF+HS+K   I+HGDLKP N+LLD N+V KL+ FG
Sbjct: 584 SCRDNSPPLSWQTRIRIAAELCSVLIFLHSSKQHSIVHGDLKPANILLDENFVTKLSDFG 643

Query: 607 SCHFLPHDEKLSYNENI----PAKYGVKSNHEF----PLTTKLDVFSFGMVLLSLLTGKS 666
            C  L H E  S N  I    P       + EF     LT K DV+SFG++LL LLT + 
Sbjct: 644 ICRLLHHKEGSSNNTAICRTDPKGTFSYMDPEFLSTGELTPKSDVYSFGIILLRLLTARQ 703

Query: 667 YLRMKEDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWR 726
            L + ++VQ  +++  LK +LDP AGDWPFVQAEQLA LALRCC  +   RPDL+S+VWR
Sbjct: 704 PLGITKEVQCELDKGNLKTLLDPLAGDWPFVQAEQLAHLALRCCEMSRKNRPDLLSEVWR 763

Query: 727 VLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWL 786
           VLEPMKASCGGS    L   +   QPP YF+CPIFQEVM DPHVAADG+TYEAEAL+GWL
Sbjct: 764 VLEPMKASCGGSSFFQLG-SEEHFQPPSYFICPIFQEVMRDPHVAADGYTYEAEALKGWL 823

Query: 787 DSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQ 804
           DSGH+TSPMTNL+L H++L+PNRALRS IQEWL QQ
Sbjct: 824 DSGHDTSPMTNLKLAHRDLIPNRALRSAIQEWLQQQ 836

BLAST of Cp4.1LG12g03650 vs. TrEMBL
Match: D7UCB8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0046g02480 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 1.7e-224
Identity = 425/846 (50.24%), Postives = 576/846 (68.09%), Query Frame = 1

Query: 7   DDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEV 66
           +D +++AVG +V E +  LV+A+++ GG+RI I+HVH+PA++IP +G +F AS +KE+EV
Sbjct: 45  EDKIFVAVGKEVKESKSILVWALQNSGGKRISIVHVHQPAQMIPIMGGKFPASKLKEQEV 104

Query: 67  NAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAA 126
            AYR+ ERQD +KIL+ +L +C +AGVRAEKLY+ES+ ++KGI+EL+S HGI++L++GAA
Sbjct: 105 KAYRDLERQDMHKILNDYLLICRKAGVRAEKLYIESENVEKGILELISEHGIKKLVVGAA 164

Query: 127 ADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPPSPQS 186
           ADK+YSR+M + +SKKA YV  KAP FC I FVC G LI TRE     A +++  P  Q 
Sbjct: 165 ADKHYSRRMLEPKSKKAAYVRDKAPLFCHIWFVCRGHLIYTREGSLNGADIELRTPPSQQ 224

Query: 187 QNVNGACWRP-------VESGQFNGSRIQSPSIVLER-----LSISKLVSNDS--GTSSP 246
            + N    +        V  GQ + S++ +P   L R     + I+ L S D   G S+P
Sbjct: 225 ASPNNETGQSNTFRSMSVSLGQNHPSKLVNPGQDLPRTMSVPVRITVLSSPDGTGGVSAP 284

Query: 247 FER--------------SYSPSSSSGCLDAASSRT--EEDEYGVGLSSPLLLKDVAPNSS 306
           + R                SPS +SG    +S     E +E G+   +  + K    +SS
Sbjct: 285 WSRMGREGSSDYWDGISKRSPSQASGFSTCSSGDMAGEVNEDGLESRASPVAKQALHHSS 344

Query: 307 PLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELGNAIFRARE-- 366
           P  +         ++ +Y QLE+++ EA N++REAF+E+++R++AE +   AI RA+E  
Sbjct: 345 PPSV--------LEENIYDQLEQAMVEAENSRREAFQESLRRSKAEKDAIEAIRRAKEAE 404

Query: 367 ---------RKDVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVK 426
                    R+D+EEAL  + +ELE+++N+  ++MEEL++S N    LENQIA S+Q+VK
Sbjct: 405 RSFSEELKLRRDIEEALQAQGKELESLRNKQQEIMEELKISLNHKSLLENQIADSEQVVK 464

Query: 427 ELEQKILSAIELLHNYKNDRDVLLKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSE 486
           ELE+KI++A+ELL NYK +RD L  +RD A+K  ++++ +   A T HT Q F+EFSF+E
Sbjct: 465 ELEEKIIAAVELLQNYKKERDELQIERDNAIKTAEELKKKG--ASTSHTPQYFAEFSFAE 524

Query: 487 IAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRH 546
           I +AT+ FDPS+KIGEG +GS+YKG L +T+V IKM  S S Q P EFQ+EVD+LS LRH
Sbjct: 525 IEKATQNFDPSVKIGEGGYGSIYKGCLRHTQVAIKMLHSDSFQGPTEFQQEVDILSKLRH 584

Query: 547 PNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHS 606
           PN+ TLIG CPEA  L+Y+Y PNGSLEDRL C+DN+ PLSW+ RIRIA+ELCS LIF+HS
Sbjct: 585 PNLVTLIGACPEAWALIYEYLPNGSLEDRLNCRDNTPPLSWQARIRIAAELCSVLIFLHS 644

Query: 607 NKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENIPAKYGVKSNHEF- 666
           N    I+HGDLKP N+LLDAN+  KL+ FG C  + HD   S +  +  + G K    + 
Sbjct: 645 NNPDSIVHGDLKPSNILLDANFGSKLSDFGICRVISHDGNSSNSATMCCRTGPKGTFAYM 704

Query: 667 --------PLTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAVEERKLKNVLDPRAGDWP 726
                    LT K DV+SFG++LL LLTGK  + + ++VQ A+++  L  +LDP AGDWP
Sbjct: 705 DPEFLSSGELTVKSDVYSFGIILLRLLTGKPAIGITKEVQHALDQGNLNTLLDPLAGDWP 764

Query: 727 FVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPPPY 786
           FVQA+QLA +ALRCC  N   RPDLVS+VWRVLEPMK SCG S S      + + Q PPY
Sbjct: 765 FVQAKQLALMALRCCEMNRKSRPDLVSEVWRVLEPMKVSCGASSSSFRVGSEERGQIPPY 824

Query: 787 FLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRSVI 803
           F+CPIFQE+M+DP VAADGFTYEAEALRGWLD GH+TSPMTNL+L H NLVPNRALRS I
Sbjct: 825 FICPIFQEIMQDPCVAADGFTYEAEALRGWLDGGHSTSPMTNLKLGHLNLVPNRALRSAI 880

BLAST of Cp4.1LG12g03650 vs. TrEMBL
Match: W9QPA1_9ROSA (U-box domain-containing protein 33 OS=Morus notabilis GN=L484_012411 PE=4 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 1.1e-223
Identity = 445/857 (51.93%), Postives = 567/857 (66.16%), Query Frame = 1

Query: 7   DDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEV 66
           ++ +Y+A+G DV E +  LV+A+++ GG++ICILHVH PA++IP LGT+F ASS+K++EV
Sbjct: 43  ENTIYVAIGKDVKESKSTLVWALQNSGGKKICILHVHVPAQMIPLLGTRFPASSLKDQEV 102

Query: 67  NAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAA 126
            AYRE ERQ+ NKILD +L LC + GV+AEKL++E D I+KGI+EL+S H IR+L+MGAA
Sbjct: 103 RAYREIERQNMNKILDEYLLLCRRMGVQAEKLFIEKDCIEKGIIELISQHNIRKLVMGAA 162

Query: 127 ADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPPSP-- 186
           ADKYYSRK+  ++SKKA++V  +AP  C I F C G LI TR     E       PSP  
Sbjct: 163 ADKYYSRKVMDLKSKKAIFVREQAPGSCHINFTCKGHLIHTRSGDDIEFRSLSVQPSPNT 222

Query: 187 --------QSQNVN-GACWRPV--ESGQFNGSRIQSPSIVLERLSISKLVSNDS--GTSS 246
                   +SQ+VN     RP      Q    R++S +I     +++ + S D   G S+
Sbjct: 223 ETGQINHLRSQSVNLRQNSRPALTSPAQELFRRVRSANIEKSGGTMTDVSSLDGTEGLST 282

Query: 247 PFERS----------------------YSPSSSSGCLDAAS---SRTEEDEYGVGLSSPL 306
           P  R+                      YS  SS+  LD A    +R E  E G  +S+  
Sbjct: 283 PRNRTDSVGSYDEWYGLPRGSPSPGSGYSTCSSTPMLDVALVPFARNEGSETGSEISALT 342

Query: 307 LLK-DVAPNSSPLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENEL 366
             K D+  +S P  L G     S DD LY  LE+S+ EA NAKREAF+EAV+R +AE + 
Sbjct: 343 HSKEDINHSSPPSVLEG-----SIDDTLYDHLEQSLAEAENAKREAFQEAVRRGKAEKDA 402

Query: 367 GNAIFRARE-----------RKDVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELE 426
            NAI RA+E           RKD EEAL +EREEL  +K Q +++MEEL+ ++++   LE
Sbjct: 403 INAIRRAKESEILYAEELKHRKDTEEALRREREELNKMKKQRDEVMEELRAAQSQKTSLE 462

Query: 427 NQIAGSDQMVKELEQKILSAIELLHNYKNDRDVLLKQRDEALKEVDDIRTRQVEARTKHT 486
           NQIA SD+MVK LEQKI+SA++LL +YK +R+    +RD AL+E +++R +Q E  +   
Sbjct: 463 NQIAESDKMVKNLEQKIISAVDLLQSYKKEREEFQMERDNALREAEELRRKQGEDTSTQM 522

Query: 487 AQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQ 546
            Q FSEFS SEI EAT  F+PSLKIGEG +GS+YKGLL +T+V                 
Sbjct: 523 PQFFSEFSCSEIEEATDNFNPSLKIGEGGYGSIYKGLLRHTQV----------------- 582

Query: 547 REVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIAS 606
              DVLS LRHPN+ TL+G CPEA  LVY+Y PNGSLEDRL CK+N+ PLSW+ RIRIA+
Sbjct: 583 ---DVLSKLRHPNLVTLVGACPEAWTLVYEYLPNGSLEDRLCCKENTPPLSWQARIRIAT 642

Query: 607 ELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENIPA 666
           ELCS LIF+HS+K   I+HGDLKP N+LLDAN+V KL+ FG C  L H E  S N  I  
Sbjct: 643 ELCSVLIFLHSSKPHSIVHGDLKPANILLDANFVSKLSDFGICRLLSHGESSSNNTTICC 702

Query: 667 KYGVKSNHEF---------PLTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAVEERKLK 726
           +   K    +          LT+K DV+SFG++LL LLTG+  L + ++VQ+A++  KLK
Sbjct: 703 RTDPKGTFAYMDPEFLSTGELTSKSDVYSFGIILLRLLTGRPALGITKEVQYALDNGKLK 762

Query: 727 NVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLS 786
            +LDP AGDWPFVQAEQLA+LALRCC  N   R DL SDVWRVLEPM+ASCGGS S  L 
Sbjct: 763 TLLDPLAGDWPFVQAEQLARLALRCCEMNRKSRADLGSDVWRVLEPMRASCGGSPSFRLG 822

Query: 787 FGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQN 803
            G+   QPPPYF+CPIFQEVM+DPHVAADGFTYEAEALRGWLDSGH+TSPMTN +LEH N
Sbjct: 823 SGE-HFQPPPYFICPIFQEVMQDPHVAADGFTYEAEALRGWLDSGHDTSPMTNHKLEHCN 873

BLAST of Cp4.1LG12g03650 vs. TAIR10
Match: AT2G45910.1 (AT2G45910.1 U-box domain-containing protein kinase family protein)

HSP 1 Score: 711.1 bits (1834), Expect = 7.9e-205
Identity = 381/809 (47.10%), Postives = 539/809 (66.63%), Query Frame = 1

Query: 7   DDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEV 66
           D+ +++AV   V + +  L++A+++ GG++IC++HVH+P+++IP +G +F   ++KE+EV
Sbjct: 44  DEKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEV 103

Query: 67  NAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAA 126
             +RE+ER+  + ILD +L +C Q GVRAEK+++E + I+ GIV+L+S  GIR+L+MGAA
Sbjct: 104 RVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVMGAA 163

Query: 127 ADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPPSPQ- 186
           AD++YSR+MT ++S+KA++V  +AP  CQI F C G LI TREA   +   +   P P  
Sbjct: 164 ADRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPRPSI 223

Query: 187 SQNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPSSSSGC 246
           S +     +   ES   + SR+QS        S+ +LVSN S T        S   S G 
Sbjct: 224 SASDLLQTFSTPESEHQHISRVQSTD------SVQQLVSNGSSTEQ------SGRVSDGS 283

Query: 247 LDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQQDESADDILYIQLEKSITEAA 306
           L+      E D   V  S+ ++        S  H S     +  DD   +++ K+ +EA 
Sbjct: 284 LNTDEEERESDGSEVTGSATVM--------SSGHSSPSSFPDGVDDSFNVKIRKATSEAH 343

Query: 307 NAKREAFREAVKRAQAENELGNAIFRARE-----------RKDVEEALSKEREELENVKN 366
           ++K+EAF E ++R +AE    +AI RA++           RKD E A++KE+E    +KN
Sbjct: 344 SSKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEELKRRKDTEIAVAKEKERFITIKN 403

Query: 367 QVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDVLLKQRDE 426
           +   +MEELQ +  +   LE+QIA SD  +++L QK+  A++LL   +++R+ L  +RD 
Sbjct: 404 EQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIAVKLLQKLRDEREELQTERDR 463

Query: 427 ALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYN 486
           AL+E +++R+   E  T    Q F++FSFSEI EAT  FD +LKIGEG +GS+Y GLL +
Sbjct: 464 ALREAEELRSH-AETSTLQLPQYFTDFSFSEIEEATNHFDSTLKIGEGGYGSIYVGLLRH 523

Query: 487 TEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDR 546
           T+V IKM   +S Q PVE+Q+EVDVLS +RHPNI TLIG CPE   LVY+Y P GSLEDR
Sbjct: 524 TQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLIGACPEGWSLVYEYLPGGSLEDR 583

Query: 547 LACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGF 606
           L CKDNS PLSW+ R+RIA+E+C+AL+F+HSNK   ++HGDLKP N+LLD+N V KL+ F
Sbjct: 584 LTCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSLVHGDLKPANILLDSNLVSKLSDF 643

Query: 607 GSCHFLPHDEKLSYNENIP---AKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYLRM 666
           G+C  L  +   S   ++    A    +++    LT K DV+SFG++LL LLTG+  LR+
Sbjct: 644 GTCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTPKSDVYSFGIILLRLLTGRPALRI 703

Query: 667 KEDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEP 726
             +V++A++   L ++LDP AGDWPFVQAEQLA+LALRCC   S  RPDL ++VWRVLEP
Sbjct: 704 SNEVKYALDNGTLNDLLDPLAGDWPFVQAEQLARLALRCCETVSENRPDLGTEVWRVLEP 763

Query: 727 MKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH 786
           M+AS GGS S  L   + ++  PPYF+CPIFQEVM+DPHVAADGFTYEAEA+R WLDS H
Sbjct: 764 MRASSGGSSSFHLGRNEHRI-APPYFICPIFQEVMQDPHVAADGFTYEAEAIRAWLDSEH 823

Query: 787 NTSPMTNLRLEHQNLVPNRALRSVIQEWL 801
           +TSPMTN++L H +L+ N ALRS IQEWL
Sbjct: 824 DTSPMTNVKLSHTSLIANHALRSAIQEWL 830

BLAST of Cp4.1LG12g03650 vs. TAIR10
Match: AT3G49060.1 (AT3G49060.1 U-box domain-containing protein kinase family protein)

HSP 1 Score: 507.7 bits (1306), Expect = 1.3e-143
Identity = 300/813 (36.90%), Postives = 475/813 (58.43%), Query Frame = 1

Query: 7   DDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEV 66
           D+ +++AV  DV   +  +++A ++  G++IC+L+VH  A+   +   + +  S K+ +V
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDV 73

Query: 67  NAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAA 126
                 E+   +++++ +L L  +  ++ +KL +    I++ IVEL++ H I+ L+MGAA
Sbjct: 74  KVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAA 133

Query: 127 ADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPPSPQS 186
           +DK+YS KMT ++SKKA++V  KAP  C I F+C G LI TR +     +    PP  Q 
Sbjct: 134 SDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIFTRASNDDSNNRQTMPPLVQL 193

Query: 187 QNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGT--SSPFERSYSPSSSSG 246
            + N          +   S     S +  RL   + +    G   +   ER      +  
Sbjct: 194 DSDN----------ETRKSEKLESSYMRRRLRYWRSLLEQDGEKDTGQLEREKVEPRAPP 253

Query: 247 CLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQQDESADDIL--YIQLEKSIT 306
              + SS +  +  G    SP L+     +S  L+ S  ++ E   D+     + +K++ 
Sbjct: 254 LFSSGSSSSFGEPVGPEPVSPELV-----DSDTLNTSNVEEKEREGDVARKVHRYDKAMH 313

Query: 307 EAANAKREAFREAVKRAQAENELGNAIFRAR-----------ERKDVEEALSKEREELEN 366
           +   + R  + EA K+ + +     A+ +A+           +RK +EE L KE+ E++ 
Sbjct: 314 DIGQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKM 373

Query: 367 VKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDVLLKQ 426
           V  Q N  M+ELQ+ + R L+LE+Q+     + KE  +K  +A+ELL +++  RD +   
Sbjct: 374 VIEQNNGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFRQKRDEIRID 433

Query: 427 RDEALKEVDDIRTRQVEART-KHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKG 486
            + A+KEV+ +R R V+  T + +     ++SF EI EAT +FDPS K+GEG +GS+YKG
Sbjct: 434 HENAVKEVNALR-RLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKG 493

Query: 487 LLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGS 546
            L + +V +KM  S+   N  EF+R V++LS +RHPN+ TL+G CPE+  L+Y Y PNGS
Sbjct: 494 NLQHLQVAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGACPESRSLIYQYIPNGS 553

Query: 547 LEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPK 606
           LED  + ++N   LSW++RIRIASE+CSAL+F+HSN  C IIHG+LKP  +LLD+N V K
Sbjct: 554 LEDCFSSENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTK 613

Query: 607 LAGFGSCHFLPHDEKLSYNENIPAKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYLR 666
           +  +G    +P D     + ++   Y V       +T + D+++FG++LL LLT +    
Sbjct: 614 INDYGISQLIPIDGLDKSDPHVDPHYFVSRE----MTLESDIYAFGIILLQLLTRRPVSG 673

Query: 667 MKEDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLE 726
           +  DV+ A+E   +  VLD  AGDWP  + ++LA +A+RCC +N M RPDL + V R ++
Sbjct: 674 ILRDVKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDL-AVVLRFID 733

Query: 727 PMKA-SCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDS 786
            MKA     S + S +  ++  +PP ++LCPIFQEVM+DP +AADGFTYEAEA+R WL +
Sbjct: 734 RMKAPEVPSSETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLAN 793

Query: 787 GHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQ 803
           GH+TSPMTNL++E  NL+PN AL   IQ+W +Q
Sbjct: 794 GHDTSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of Cp4.1LG12g03650 vs. TAIR10
Match: AT4G25160.1 (AT4G25160.1 U-box domain-containing protein kinase family protein)

HSP 1 Score: 301.2 bits (770), Expect = 1.9e-81
Identity = 233/794 (29.35%), Postives = 373/794 (46.98%), Query Frame = 1

Query: 39  ILHVHEPAKLIPF-LGTQFLASSMKEKEVNAYRERERQDANKILDGFLFLCHQAGVRAEK 98
           +LH+H     +P  +G     S +++  V AYR+     + ++L  +  L  +  V  E 
Sbjct: 53  LLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEV 112

Query: 99  LYVESDKIKKGIVELVSLHGIRRLIMGAAADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQ 158
           L +ESD +   I E V+   I R+++G ++  ++SRK        A+      P FC + 
Sbjct: 113 LVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSFFSRKADICSVISALM-----PNFCTVY 172

Query: 159 FVCNGQLIRTREACPQEAHVDIPPPSPQSQNVNGACWRPVESGQ-----FNGSRIQSPSI 218
            V  G+L   R +   + +  I     +  N +     P           + S+ +  S+
Sbjct: 173 VVSKGKLSCVRPS-DSDGNATIREDGSERTNSSSGSSGPTSDSSDVMSSAHDSQSRPLSL 232

Query: 219 VLERLSISKLVSNDSGTSSPFERSYSPSSSSGC--LDAASSRTEEDEYGVGLSSPLLLKD 278
            + R+     ++  +  S P E S   S  + C  LDA  +R   D   +  SS      
Sbjct: 233 PVRRMQHFPAIAGQA--SVPMETSSVGSDETRCMSLDAEEAR---DVSSINRSSTDTTSR 292

Query: 279 VAPNSSPLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELGNAIF 338
             P              S+ +  Y       + +          A ++A   ++  +   
Sbjct: 293 WTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTHSRASQQASNMSDALSEQS 352

Query: 339 RARERKDVEEALSKEREELENVKNQVN-KMMEELQVSRNRG----LELENQIAGSDQMVK 398
               + ++   + K R EL +V+        E    SR  G      LE  I   +  +K
Sbjct: 353 YTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGELNQRRLEEAIKLEELKLK 412

Query: 399 ELEQKILSAIELLHNYKNDRDV----------LLKQRDEALKEVDDIRTRQVEARTKHTA 458
           E E + L+  E  +  K  RD           + ++R+   K   D + ++    T  + 
Sbjct: 413 EYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKSARDTKEKEKLEGTLGSP 472

Query: 459 QV-FSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQ 518
           Q+ +  F++ EI  AT  F   LKIG G +G++YK  L++T   +K+  S   Q   +FQ
Sbjct: 473 QLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQ 532

Query: 519 REVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIAS 578
           +E+++LS +RHP++  L+G CPE   LVY+Y  NGSLEDRL   +NS PL W  R RIA 
Sbjct: 533 QELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAW 592

Query: 579 ELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFL---PHDEKLS-YNE 638
           E+ +AL+F+H +K   IIH DLKP N+LLD N+V K+   G    +   P   K + Y +
Sbjct: 593 EVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQ 652

Query: 639 NIPAKYGVKSNHEFP----LTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAVEER-KLK 698
             P       + E+     +++K D++SFGM+LL LLT K  + +   V+ A++   +  
Sbjct: 653 TSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAIALTHFVESAMDSNDEFL 712

Query: 699 NVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLS 758
            +LD +AG+WP  +  +LA LAL C       RPDL   +   LE +K     +     S
Sbjct: 713 KILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPALENLKKV---AEKARNS 772

Query: 759 FGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQN 800
           F  +  QPP +F+CP+ ++VM +P VAADG+TY+  A+  WL   HNTSPMT+  L  +N
Sbjct: 773 FSGVSTQPPTHFICPLLKDVMNEPCVAADGYTYDRHAIEEWLKE-HNTSPMTDSPLHSKN 831

BLAST of Cp4.1LG12g03650 vs. TAIR10
Match: AT5G51270.1 (AT5G51270.1 U-box domain-containing protein kinase family protein)

HSP 1 Score: 279.6 bits (714), Expect = 5.9e-75
Identity = 197/628 (31.37%), Postives = 318/628 (50.64%), Query Frame = 1

Query: 225 NDSGTSSPFERSYSPSSSSGCLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQ 284
           ND+   S FER+ S  SS                G G +S ++   +  N   L     Q
Sbjct: 214 NDTSIESGFERTSSSCSS----------------GSGANSDVMSNALKSNPHTLSNKRMQ 273

Query: 285 QDESADDILYIQLEKSITEAANAKR---EAFREAVKRAQAENELG---NAIFRA-RERKD 344
              +    + + +E S TE+   K+   +A  EA KR+  E       N  FR   ERKD
Sbjct: 274 NLPTIVRGVSVPMETSSTESDETKKRSSDAAEEASKRSSPETSRSVSWNPQFRDFDERKD 333

Query: 345 VEEALSKERE-------------ELENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMV 404
              ++S   E             + ++  N+++K+  EL+ +       + +   + + +
Sbjct: 334 AMSSMSSNFEYGNVVTPLGHYFTDNQDTLNEISKLRAELRHAHEMYAVAQVETLDASRKL 393

Query: 405 KELEQKILSAIELLHNYKN-DRDVLLKQRDEALKEVDDIRTRQVEARTKHTAQV------ 464
            EL+ + L+ +E  H  K   +    K   +  +E +  + R+ E +  H A+       
Sbjct: 394 NELKFEELTLLE--HETKGIAKKETEKFEQKRREEREAAQRREAEMKATHEAKEKEKLEE 453

Query: 465 ---------FSEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQ 524
                    + EF++ EI  AT  F   LKIG G +G +YK  L++T   +K+  S    
Sbjct: 454 SSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLHSAESS 513

Query: 525 NPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKT 584
              +F +E+++LS +RHP++  L+G CP+   LVY+Y  NGSLEDRL   ++S P+ W  
Sbjct: 514 LSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQPIPWFV 573

Query: 585 RIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLS- 644
           R+RIA E+ SAL+F+H +K   IIH DLKP N+LL+ N+V K+   G    +   + LS 
Sbjct: 574 RLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAADPLST 633

Query: 645 ----YNENIPAKYGVKSNHEFP----LTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAV 704
               Y +  P       + E+     ++ K DV++FGM++L LLTG+  + +   V+ A+
Sbjct: 634 KFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTYTVETAM 693

Query: 705 E---ERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASC 764
           E   + +L  +LD +AG+WP  +  QLA LAL+C    S  RPDL   +  VLE +K   
Sbjct: 694 ENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLESLKKVA 753

Query: 765 GGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPM 805
             + +   S      QPP +F CP+ ++VM++P +AADG+TY+  A+  W+++ H TSP+
Sbjct: 754 DKARN---SLSAAPSQPPSHFFCPLLKDVMKEPCIAADGYTYDRRAIEEWMEN-HRTSPV 813

BLAST of Cp4.1LG12g03650 vs. TAIR10
Match: AT5G61560.1 (AT5G61560.1 U-box domain-containing protein kinase family protein)

HSP 1 Score: 278.1 bits (710), Expect = 1.7e-74
Identity = 225/790 (28.48%), Postives = 381/790 (48.23%), Query Frame = 1

Query: 57  LASSMKEKEVNAYRER-ERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSL 116
           ++++ K+   + Y++  +R+    +L       H+  V+ + + +ESD I   I + V  
Sbjct: 51  VSTTRKDLTTSVYKKDVDRKTREMLLPSRDMFVHRE-VQLDIMVLESDDIADAISKAVQD 110

Query: 117 HGIRRLIMGAAADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREAC--PQ 176
           HGI  L++GA++   +S K+   RS  +  +    P FC +  +  G+L+  R++    +
Sbjct: 111 HGISELVIGASSSIIFSWKLK--RSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTE 170

Query: 177 EAHVDIPPPSPQSQNVNGACWRPVESGQFNGS-----RIQSPSIVLERLSISKLVSNDSG 236
            +  D    S  S + +        S QF+ +     RIQ+ + V +++  +    N+  
Sbjct: 171 TSIADDRSESRFSSDSHSGTVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNEP 230

Query: 237 TSSPFERS----------------YSPSSSS---GCLDAASSRTEEDEYGVGLSSPLLLK 296
                 R+                Y  SSS    G  D +S R+ + E     SS     
Sbjct: 231 HHHHHNRAGSLDVDESKLLNQKGFYRTSSSGIGYGGSDISSWRSSQMEEA---SSSSTYS 290

Query: 297 DVAPNSSPLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELGNAI 356
           D   +SS +H                +LEK   E  + K          A A++E+ +A 
Sbjct: 291 DPTSSSSQIHKD-------------FELEKLKIELRHIKG-------MYAVAQSEVIDA- 350

Query: 357 FRARERKDVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQ 416
             +++ +D+ +  S+E   L+N+  +  +  E +++ R R  + EN+     + ++   +
Sbjct: 351 --SKKMQDLNQRRSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECIERETE 410

Query: 417 KILSAIELLHNYKNDRDVLLKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEA 476
           + L A       + +     KQR E   E   ++ +Q           + +F + EI EA
Sbjct: 411 ERLEAEARAEEVRKE-----KQRLEDALEGGPLQRQQ-----------YMKFEWEEIVEA 470

Query: 477 TRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIA 536
           T  F   LKIG G +GS+Y+  L++T V +K+  S       +F +E+++LS +RHP++ 
Sbjct: 471 TSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQFHQELEILSKIRHPHLL 530

Query: 537 TLIGVCPEACVLVYDYFPNGSLEDRLACK------DNSSPLSWKTRIRIASELCSALIFI 596
            L+G CPE   LVY+Y  NGSLE+RL  +          PL W  R RIA E+ SAL F+
Sbjct: 531 LLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRWFERFRIAWEIASALYFL 590

Query: 597 HSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFL---PHDEKLSYNENIPAKYGVKS 656
           H+N+   I+H DLKP N+LLD N V K+   G    +   P      +NE  P       
Sbjct: 591 HTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSHASTVFNETGPVGTFFYI 650

Query: 657 NHEFP----LTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAVEER--KLKNVLDPRAGD 716
           + E+     +T + D+++FG++LL L+T +S + +   ++ A+ ++  K   +LD  AGD
Sbjct: 651 DPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSIEKALRDQTGKFTEILDKTAGD 710

Query: 717 WPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMK--ASCGGSVSVSLSFGDLQVQ 776
           WP  +A+++  + LRC       RPDL  ++  VLE +K  AS   ++    +  D    
Sbjct: 711 WPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERLKEVASIARNMFAD-NLIDHHHN 770

Query: 777 PPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRAL 803
            P +F CPI ++VME+P VA+DG+TYE  A++ WL   H  SPMT+L     +L+PN +L
Sbjct: 771 APTHFYCPITKDVMENPCVASDGYTYEKRAIKEWLQKNHK-SPMTDLPFPSDSLLPNHSL 793

BLAST of Cp4.1LG12g03650 vs. NCBI nr
Match: gi|700190457|gb|KGN45661.1| (hypothetical protein Csa_6G004520 [Cucumis sativus])

HSP 1 Score: 1294.6 bits (3349), Expect = 0.0e+00
Identity = 650/815 (79.75%), Postives = 717/815 (87.98%), Query Frame = 1

Query: 1   MAGSCGDDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASS 60
           MAGS G+D+VY+AVGND+NEC+LNLVYAIKH GGRRICILHVHEPAK IP LGT+FLASS
Sbjct: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60

Query: 61  MKEKEVNAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRR 120
           MKE+EVNAYRE ERQDA+KILD FL LCHQAG RAEKLY ESDKIKKGIVELVSLHGIR 
Sbjct: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120

Query: 121 LIMGAAADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIP 180
           L+MGAAADKYYSRKMTKIRS+KAMYVHL A A CQIQFVC GQLIR REACPQE H DI 
Sbjct: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180

Query: 181 PPSPQSQNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPS 240
           P SPQSQN+NG  WR  + G FNG    SP+IV+ERL+IS+ +SN SG  SPFERSY+ S
Sbjct: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240

Query: 241 SSSGCLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQQDESADDILYIQLEKS 300
            SSG +D ASSRTEED YG+GL+SPLLL + A  +SP H SGFQQD SADD LYIQLEK+
Sbjct: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGFQQDGSADDSLYIQLEKA 300

Query: 301 ITEAANAKREAFREAVKRAQAENELGNAIFRA-----------RERKDVEEALSKEREEL 360
           I +AANA+REAFREA+KRA+AE EL  AI  A           R RKD EE LSKEREEL
Sbjct: 301 IADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREEL 360

Query: 361 ENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDVLL 420
           ++VKNQVN+MM+ELQ++RN GL+LENQIA SD+MVKELEQKILSAIELLHNYKNDRD LL
Sbjct: 361 DDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDELL 420

Query: 421 KQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYK 480
           KQRDEALKE+DDIRTRQVEA ++H+AQ+ SEFSFSEI EATRKFDPSLKI    +GSMYK
Sbjct: 421 KQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMYK 480

Query: 481 GLLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNG 540
           GLLYNTEV IKM  SH+LQNPV+FQREVD LS LRHPNIATLIGVCPEAC+LVYDYFPNG
Sbjct: 481 GLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPNG 540

Query: 541 SLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVP 600
           +LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHSNKICK +HGD+KP N+LLDANYVP
Sbjct: 541 NLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYVP 600

Query: 601 KLAGFGSCHFLPHDEKLSYNENIPAKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYL 660
           KLAGFG+CHFL HDEKLSYNEN+   Y  K NHEFPLTT+LD++SFG VLLSLLTGKSYL
Sbjct: 601 KLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYL 660

Query: 661 RMKEDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVL 720
           RMKEDVQ A+++RKLK+VLDPRAGDWPFVQAEQLAQLALRCC+ +SMYRPDLVSDVWRVL
Sbjct: 661 RMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVL 720

Query: 721 EPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 805
           GH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 813

BLAST of Cp4.1LG12g03650 vs. NCBI nr
Match: gi|659116727|ref|XP_008458227.1| (PREDICTED: U-box domain-containing protein 33-like [Cucumis melo])

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 649/815 (79.63%), Postives = 718/815 (88.10%), Query Frame = 1

Query: 1   MAGSCGDDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASS 60
           MAGS G+D VY+AVGND+NEC+LNLVYAIKH GGRRICILHVHEPAK IP LGT+FLASS
Sbjct: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60

Query: 61  MKEKEVNAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRR 120
           M+E+EVNAYRE ERQDANKILD FL LCHQAG +AEKLY ESDKIKKGIVELVSLHGIR 
Sbjct: 61  MEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDKIKKGIVELVSLHGIRS 120

Query: 121 LIMGAAADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIP 180
           L+MGAAADKYYSRKMTKIRS+KAMYVHL A A CQIQFVC GQLIR REACPQEAH DI 
Sbjct: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQEAHADIS 180

Query: 181 PPSPQSQNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPS 240
           P SPQSQN+NG  WR  + GQFNG    SPSIV+ERL+IS+ +SN SGT SPFERSY+ S
Sbjct: 181 PSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLTISETISNASGTWSPFERSYATS 240

Query: 241 SSSGCLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSGFQQDESADDILYIQLEKS 300
            SSGC D ASSR+EED YG+GLSSPL L + APN+SP HLSGFQQD +A+D LYIQLEK+
Sbjct: 241 PSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSP-HLSGFQQDGNANDSLYIQLEKA 300

Query: 301 ITEAANAKREAFREAVKRAQAENELGNAIFRA-----------RERKDVEEALSKEREEL 360
           IT+AANA+REAFREA+KRA+AE EL  AI  A           R RK+ EEALSKEREEL
Sbjct: 301 ITDAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKEAEEALSKEREEL 360

Query: 361 ENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDVLL 420
           +NVKNQVN+MM++LQ+++N  L+LENQIA SD+MVKELEQKILSAI+LLHNYKNDRD LL
Sbjct: 361 DNVKNQVNEMMKDLQIAQNNELKLENQIAQSDEMVKELEQKILSAIQLLHNYKNDRDELL 420

Query: 421 KQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMYK 480
           KQRDEAL+E+DDIR RQVEAR++ +AQ+ SEFSFSEI EATRKFDPSLKI EG +GSMYK
Sbjct: 421 KQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATRKFDPSLKIVEGENGSMYK 480

Query: 481 GLLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPNG 540
           GLLYNTEV IKM  SH+LQNPVEFQREVD LS LRHPN+ATLIGVCPEAC+LVYDYFPNG
Sbjct: 481 GLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPEACILVYDYFPNG 540

Query: 541 SLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYVP 600
           +LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHSNKICKI+HGD+KP NVLLDANYVP
Sbjct: 541 NLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMKPSNVLLDANYVP 600

Query: 601 KLAGFGSCHFLPHDEKLSYNENIPAKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYL 660
           KLAGFG CHFL HDEK SYNEN+   +    NHEFPLTT  DV+SFGMVLLSLLTG+SYL
Sbjct: 601 KLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTTAFDVYSFGMVLLSLLTGQSYL 660

Query: 661 RMKEDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVL 720
           RM+EDVQ A+++RKLK+VLDPRAGDWPFVQAEQLAQLALRCCN +S YRPDLVSD WRVL
Sbjct: 661 RMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTDSTYRPDLVSDAWRVL 720

Query: 721 EPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780
           EPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDS
Sbjct: 721 EPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDS 780

Query: 781 GHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 805
           GH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 GHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814

BLAST of Cp4.1LG12g03650 vs. NCBI nr
Match: gi|778721943|ref|XP_011658378.1| (PREDICTED: U-box domain-containing protein 33-like [Cucumis sativus])

HSP 1 Score: 1285.4 bits (3325), Expect = 0.0e+00
Identity = 650/828 (78.50%), Postives = 717/828 (86.59%), Query Frame = 1

Query: 1   MAGSCGDDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASS 60
           MAGS G+D+VY+AVGND+NEC+LNLVYAIKH GGRRICILHVHEPAK IP LGT+FLASS
Sbjct: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASS 60

Query: 61  MKEKEVNAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRR 120
           MKE+EVNAYRE ERQDA+KILD FL LCHQAG RAEKLY ESDKIKKGIVELVSLHGIR 
Sbjct: 61  MKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRS 120

Query: 121 LIMGAAADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIP 180
           L+MGAAADKYYSRKMTKIRS+KAMYVHL A A CQIQFVC GQLIR REACPQE H DI 
Sbjct: 121 LVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADIS 180

Query: 181 PPSPQSQNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPS 240
           P SPQSQN+NG  WR  + G FNG    SP+IV+ERL+IS+ +SN SG  SPFERSY+ S
Sbjct: 181 PSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATS 240

Query: 241 SSSGCLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLSG-------------FQQDE 300
            SSG +D ASSRTEED YG+GL+SPLLL + A  +SP H SG             FQQD 
Sbjct: 241 PSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFSGTQCFYFGLSVFQGFQQDG 300

Query: 301 SADDILYIQLEKSITEAANAKREAFREAVKRAQAENELGNAIFRA-----------RERK 360
           SADD LYIQLEK+I +AANA+REAFREA+KRA+AE EL  AI  A           R RK
Sbjct: 301 SADDSLYIQLEKAIADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRK 360

Query: 361 DVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIE 420
           D EE LSKEREEL++VKNQVN+MM+ELQ++RN GL+LENQIA SD+MVKELEQKILSAIE
Sbjct: 361 DAEEELSKEREELDDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIE 420

Query: 421 LLHNYKNDRDVLLKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPS 480
           LLHNYKNDRD LLKQRDEALKE+DDIRTRQVEA ++H+AQ+ SEFSFSEI EATRKFDPS
Sbjct: 421 LLHNYKNDRDELLKQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPS 480

Query: 481 LKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCP 540
           LKI    +GSMYKGLLYNTEV IKM  SH+LQNPV+FQREVD LS LRHPNIATLIGVCP
Sbjct: 481 LKIVTDANGSMYKGLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCP 540

Query: 541 EACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDL 600
           EAC+LVYDYFPNG+LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHSNKICK +HGD+
Sbjct: 541 EACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDM 600

Query: 601 KPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENIPAKYGVKSNHEFPLTTKLDVFSFG 660
           KP N+LLDANYVPKLAGFG+CHFL HDEKLSYNEN+   Y  K NHEFPLTT+LD++SFG
Sbjct: 601 KPSNILLDANYVPKLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFG 660

Query: 661 MVLLSLLTGKSYLRMKEDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSM 720
            VLLSLLTGKSYLRMKEDVQ A+++RKLK+VLDPRAGDWPFVQAEQLAQLALRCC+ +SM
Sbjct: 661 TVLLSLLTGKSYLRMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSM 720

Query: 721 YRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGF 780
           YRPDLVSDVWRVLEPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEVMEDPHVAADGF
Sbjct: 721 YRPDLVSDVWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGF 780

Query: 781 TYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 805
           TYEAEALRGWLDSGH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 TYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 826

BLAST of Cp4.1LG12g03650 vs. NCBI nr
Match: gi|802695257|ref|XP_012083383.1| (PREDICTED: U-box domain-containing protein 33-like isoform X4 [Jatropha curcas])

HSP 1 Score: 812.0 bits (2096), Expect = 9.4e-232
Identity = 443/845 (52.43%), Postives = 585/845 (69.23%), Query Frame = 1

Query: 7   DDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEV 66
           ++ VY+AVG +V E +  L++A+++ GG++ICI+HVH+PA++IP +GT+F A+S+KE+EV
Sbjct: 44  EEKVYVAVGKNVKEYKSLLLWALQNSGGKKICIIHVHQPAQMIPLMGTKFHATSLKEQEV 103

Query: 67  NAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAA 126
            AYRE ERQ  ++ILD ++ LC Q GVRAEKL++E + I+KGI+EL+S H IR+L+MGAA
Sbjct: 104 RAYREIERQAMHEILDEYILLCWQMGVRAEKLHIEMESIEKGILELISSHCIRKLVMGAA 163

Query: 127 ADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPPSPQS 186
           ADK YSR M  I+SKKA++V L+APA CQI+F+C   +I T+E       ++I  PS Q 
Sbjct: 164 ADKRYSRNMMDIKSKKAIFVRLQAPASCQIKFICKQHVIYTKEGASDANDIEI-LPSLQR 223

Query: 187 QNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVS----NDSGTSSPFERSYSPSSS 246
             +N       E+GQ     +++ SI   RL    + S    N  G S+   R  +  +S
Sbjct: 224 NILN------TENGQ--SIHLRAQSITFGRLGGKSIPSTSPDNVGGPSTSQNRPDAEGAS 283

Query: 247 SG---CLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLS--GFQ------------- 306
           S      D + + +    +G  LS+      V+    PL  S  G +             
Sbjct: 284 SASPVSSDESDALSRTTSHGSLLSTCSSTGLVSVGLGPLIRSEGGLEISTLHQPKLDLHH 343

Query: 307 -------QDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELGNAIFRA----- 366
                  Q+ S +D LY QLE+ +TEAAN +REAF EAV+RA+AE +   AI RA     
Sbjct: 344 SSTSSVLQEGSIEDPLYDQLEQVMTEAANLRREAFEEAVRRAKAERDAIEAIRRAKASEG 403

Query: 367 ------RERKDVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKE 426
                 R RK++EE L+KE+E+LE +K++ +++MEEL ++ ++   LE+QIA ++QMV+E
Sbjct: 404 LYAEEVRRRKEIEETLAKEKEKLEKMKSERDEVMEELHIALDQKKILESQIADAEQMVRE 463

Query: 427 LEQKILSAIELLHNYKNDRDVLLKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEI 486
           LEQKI+SA+ELL NYK +RD L  +RD ALKE +++R  Q EA + H  Q FS+FSFSE+
Sbjct: 464 LEQKIISAVELLQNYKKERDELQMERDNALKEAEELRKSQAEASSSHMPQFFSDFSFSEV 523

Query: 487 AEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRHP 546
            EAT  F+PSLKIGEG +GS+YKGLL+ T+V IK+  SHSLQ P EFQ+EVDVLS +RHP
Sbjct: 524 EEATDNFNPSLKIGEGGYGSIYKGLLHYTQVAIKVLHSHSLQGPAEFQQEVDVLSKMRHP 583

Query: 547 NIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSN 606
           N+ TLIG CPEA  L+Y+Y PNGSLEDRL C+DNS PLSW+TRIRIA+ELCS LIF+HS+
Sbjct: 584 NLVTLIGACPEAWTLIYEYLPNGSLEDRLCCRDNSPPLSWQTRIRIATELCSVLIFLHSS 643

Query: 607 KICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENI-----PAKYGVKSN 666
           K   I+HGDLKP N+LLDAN+V KL+ FG C  L  +E  S N  +     P    V  +
Sbjct: 644 KPHSIVHGDLKPANILLDANFVSKLSDFGICRLLSQNEGSSNNTTMCCRTDPKGTFVYMD 703

Query: 667 HEF----PLTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAVEERKLKNVLDPRAGDWPF 726
            EF     LT K DV+SFG++LL LLTG+  L + ++VQFA+++  LKN+LDP AGDWPF
Sbjct: 704 PEFLASGELTPKSDVYSFGIILLRLLTGRPALGITKEVQFALDKGNLKNLLDPLAGDWPF 763

Query: 727 VQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPPPYF 786
           VQAEQLA LALRCC  N   RPDL ++VWRVLEPMKASCGGS    L   ++  QPPPYF
Sbjct: 764 VQAEQLAHLALRCCEMNRKSRPDLAAEVWRVLEPMKASCGGSSCFRLG-SEVHFQPPPYF 823

Query: 787 LCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRSVIQ 803
           +CPIFQE+M+DPHVAADG+TYEAEALRGWLDSGH+TSPM NL+L H NL+PN ALRS IQ
Sbjct: 824 ICPIFQEIMQDPHVAADGYTYEAEALRGWLDSGHDTSPMNNLKLAHNNLIPNHALRSAIQ 878

BLAST of Cp4.1LG12g03650 vs. NCBI nr
Match: gi|764625156|ref|XP_011469012.1| (PREDICTED: U-box domain-containing protein 33 isoform X1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 810.8 bits (2093), Expect = 2.1e-231
Identity = 441/853 (51.70%), Postives = 582/853 (68.23%), Query Frame = 1

Query: 8   DVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMKEKEVN 67
           D++Y+ VG  V E + NL++ +++ GG+R+CI+HVH+PA++IP +GT+F AS MK++EV 
Sbjct: 42  DMIYVTVGTSVKESKANLIWTLQNSGGKRVCIVHVHQPAQMIPMMGTKFPASQMKDQEVR 101

Query: 68  AYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLIMGAAA 127
           AYRE ERQ+  KIL+ +L +C Q G+RAEK+++E D I+KGIVEL+S HGI +L+MGAAA
Sbjct: 102 AYREIERQNMQKILEDYLRICRQMGIRAEKVHIEMDCIEKGIVELISQHGISKLVMGAAA 161

Query: 128 DKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQ--EAHVDIPPPSPQ 187
           DK +SRKMT ++SKKA+YV   AP  C IQFVC G LI TREA     +A V +PPP P 
Sbjct: 162 DKSHSRKMTDLKSKKAIYVREHAPVSCHIQFVCKGHLIHTREADLDAVDAQVALPPPRPS 221

Query: 188 SQNVNGACW----RPVESGQFNGSRIQSPSIVLER--------------LSISKLVSNDS 247
           + +            V  GQ N   + +P+  L R                +++ +S  S
Sbjct: 222 TNSERSPLHLRSRSSVAFGQNNRPSLSNPAQDLFRRVRSSKVDKYGGSTTDVTEGISTPS 281

Query: 248 GTSSPFERS------YSPSSSSGCLDAASS-----------RTEEDEYGVGLSSPLL-LK 307
            +    E S       S  S+SG    +SS           RTE  E G G SS L  LK
Sbjct: 282 SSRLEAELSADEWDRVSRRSTSGYSSCSSSALGDLPLIQFERTEGSENGSGNSSTLTHLK 341

Query: 308 DVAPNSSPLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELGNAI 367
           D+  +S P  L    QD + DD LY  L++ + EA NAKREAF E+++R +AE +  +AI
Sbjct: 342 DLNHSSPPSVL----QDGNVDDTLYDHLDQVMAEAENAKREAFEESIRRGKAEKDAIDAI 401

Query: 368 FRA-----------RERKDVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELENQIA 427
            RA           R+RK++E+ +++ERE LE +K Q +++ME+L+ +  + L+LE+QI+
Sbjct: 402 RRAKASEFLYNEELRQRKEIEDTVAREREVLEKMKRQRDEVMEDLRAAVEQKLQLESQIS 461

Query: 428 GSDQMVKELEQKILSAIELLHNYKNDRDVLLKQRDEALKEVDDIRTRQVEARTKHTAQVF 487
            SDQ+V+ LEQKI+SA++LL +Y+ +RD L  +RD AL+E +++R RQ EA + H  Q F
Sbjct: 462 ESDQLVQSLEQKIISAVDLLQSYRRERDELHVERDNALREAEELRKRQGEASSAHMPQFF 521

Query: 488 SEFSFSEIAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHSLQNPVEFQREVD 547
           S+FSF EI EAT+ FDPSLKIGEG +GS++KG L +TEV IKM  +HSLQ P EFQ+EVD
Sbjct: 522 SDFSFPEIEEATQNFDPSLKIGEGGYGSIFKGFLRHTEVAIKMLNAHSLQGPSEFQQEVD 581

Query: 548 VLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCS 607
           +LS LRH N+ TLIG CPEA  L+Y+Y PNGSLEDRL CKDN+SPLSW+ RIRIA+ELCS
Sbjct: 582 ILSKLRHTNLVTLIGACPEAWTLIYEYLPNGSLEDRLTCKDNTSPLSWQARIRIATELCS 641

Query: 608 ALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENIPAKYGV 667
            LIF+HS+K   I+HGDLKP N+LLDA++V KL+ FG    L   E  S N  +  +   
Sbjct: 642 VLIFLHSSKPHSIVHGDLKPSNILLDAHFVCKLSDFGISRLLSRGEGSSNNTTLCCRTDP 701

Query: 668 KSNHEF---------PLTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAVEERKLKNVLD 727
           K    +          LT K D++SFG++LL LLTG+  L + ++VQ+A++  KL+ +LD
Sbjct: 702 KGTFTYMDPEFLSSGELTPKSDIYSFGIILLRLLTGRPALGITKEVQYALDSGKLETLLD 761

Query: 728 PRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDL 787
           P AGDWPFVQAEQLA+LALRCC  +   R DLVSDVWRVLEPM+ASCG S S  L   + 
Sbjct: 762 PLAGDWPFVQAEQLARLALRCCEMSRKCRADLVSDVWRVLEPMRASCGSSSSFRLGTEE- 821

Query: 788 QVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPN 803
             QPP YF+CPIFQEVM+DPHVAADGFTYEAEALRGW+DSGH+TSPMTNL+LEH+NLVPN
Sbjct: 822 HFQPPTYFICPIFQEVMQDPHVAADGFTYEAEALRGWMDSGHDTSPMTNLKLEHKNLVPN 881