BLAST of Cp4.1LG12g00680 vs. Swiss-Prot
Match:
AEE19_ARATH (Putative acyl-activating enzyme 19 OS=Arabidopsis thaliana GN=At5g35930 PE=2 SV=1)
HSP 1 Score: 889.0 bits (2296), Expect = 5.1e-257
Identity = 479/1000 (47.90%), Postives = 630/1000 (63.00%), Query Frame = 1
Query: 130 VPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKIDLIIYSGSSF 189
+PK+ +YMPPSVEY+I+V SVLRCG AF+PLDP+WP++R+LS++S+S I L+I G S
Sbjct: 1 MPKVVALYMPPSVEYVISVFSVLRCGEAFLPLDPSWPRERVLSLISSSNISLVIACGLSS 60
Query: 190 CEDGYHLT---------------------DGFRW---LEQIRSCSTFSFTMEENSIPEHN 249
E + + F W E+ R +T P+
Sbjct: 61 VESHWLVERNVCPVLLFSMDEKLSVETGCSSFVWPCKKERQRKFCYLMYTSGSTGKPKGV 120
Query: 250 SAVDLVFPCERLLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFLSAILTSSALIIPP 309
+ + LLNRF WMQEL+P GE+ FKTS+ FIDHIQEFL AIL+S+AL+IPP
Sbjct: 121 CGTE-----QGLLNRFLWMQELYPVVGEQRFAFKTSVGFIDHIQEFLGAILSSTALVIPP 180
Query: 310 MKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQ-RLCVMQNRCSLRLLILSGEIL 369
LKE + S+++F++ YSIS+L AVPS++RA+LP LQ R + + L+L++LSGE
Sbjct: 181 FTLLKENMISIIDFLEEYSISRLLAVPSMIRAILPTLQHRGHNNKLQSCLKLVVLSGEPF 240
Query: 370 PIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEISTVPIGVPISHCD 429
P+ LW++L LLPET LNLYGSTEVSGDCTYFDC +P +L TEEI +VPIG IS+C
Sbjct: 241 PVSLWDSLHSLLPETCFLNLYGSTEVSGDCTYFDCSELPRLLKTEEIGSVPIGKSISNCK 300
Query: 430 VVVVGDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFV--HGGSL-----NAN 489
VV++GD D +GE+ V G C+ GY S I S+ +V H SL N
Sbjct: 301 VVLLGDEDKPYEGEICVSGLCLSQGYMHSS--------IESEGYVKLHNNSLCNHLTNDC 360
Query: 490 CDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDAAVVSR 549
Q+Y R GD+ RQL SGDL+F+GR+DR +K+NG+R+ALEEIE L +PD+ +A V+
Sbjct: 361 GSQLYYRTGDYGRQLSSGDLIFIGRRDRTVKLNGKRMALEEIETTLELNPDIAEAVVLLS 420
Query: 550 RSDRELEYLVAFLVL-KDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSSSGKV 609
R + EL L AF+VL K++ SD ++R+W+ K+ MIPN F ++ +P++SSGKV
Sbjct: 421 RDETELASLKAFVVLNKESNSSDGIIFSIRNWMGGKLPPVMIPNHFVLVEKLPLTSSGKV 480
Query: 610 DYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMIKEISSDDDFFTMGGNSITA 669
DYE L + + + N +Q IKKA DAL++KE+S DDDFF +GG+S+ A
Sbjct: 481 DYEALARLKCPTTGAQDMMQSNGTNSLLQNIKKAVCDALLVKEVSDDDDFFAIGGDSLAA 540
Query: 670 AHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGS-DIDINRDADSRKNLKIDRWNKFSFDD 729
AH+S+ LG+DMR +Y + SP++LL L EK+G D+ + + + KI+ N
Sbjct: 541 AHLSHSLGIDMRLIYQFRSPSRLLIYLSEKEGKLREDMQHNTTQKLDHKIESQNGNGLVS 600
Query: 730 SEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISKHNKEVSDFSINLEDIGQV 789
H + G K Q N+ R I S K KE
Sbjct: 601 RTVPLHSGVTSGPTPSKL-QCEKNNSPKRLKIDYEKFSP--KRMKE-------------- 660
Query: 790 GGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKSPRGENGSMKKLWQVHMES 849
LWDS + + CAFSR NKV E S++ PR + SM+++W+VHMES
Sbjct: 661 -NKLWDSGFSQIQCAFSRCNKVHSPESCSNEEANREYWSLEIPRNQMVSMQEIWKVHMES 720
Query: 850 CVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIECSTAIVGDFSQVV 909
CVDASPLVV K K YLFIGSHS+KF C+DAK+ S+ WE LEGRIE S +VGDFSQVV
Sbjct: 721 CVDASPLVVLKDSKTYLFIGSHSRKFSCIDAKSGSMYWETILEGRIEGSAMVVGDFSQVV 780
Query: 910 VGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDHNLYALDYVRHSC 969
+GCYKGK+YFL+FSTG++ W FQ CGE+K QPVVD LIWCGS+DH LYALDY C
Sbjct: 781 IGCYKGKLYFLDFSTGSLCWKFQACGEIKCQPVVDTSSQLIWCGSHDHTLYALDYRSQCC 840
Query: 970 VYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSAL--------TLWHYDLEAPVFGSLAI 1029
VYKL CGGSI+ SPAID LYVASTSGR A+ TLW ++LEAP+FGSL I
Sbjct: 841 VYKLQCGGSIFASPAIDEGHSSLYVASTSGRVIAVSIKDSPFHTLWLFELEAPIFGSLCI 900
Query: 1030 DPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVVPSQVLICSRNGSIYS 1088
P + NVICCLV+G V+A+ +G++ W+ +TGGPIFAG C+S V+PSQVL+C RNG +YS
Sbjct: 901 TPSTQNVICCLVDGQVIAMSPSGTIIWRYRTGGPIFAGPCMSHVLPSQVLVCCRNGCVYS 960
BLAST of Cp4.1LG12g00680 vs. Swiss-Prot
Match:
TYCC_BREPA (Tyrocidine synthase 3 OS=Brevibacillus parabrevis GN=tycC PE=1 SV=1)
HSP 1 Score: 179.5 bits (454), Expect = 2.0e-43
Identity = 148/553 (26.76%), Postives = 257/553 (46.47%), Query Frame = 1
Query: 133 IFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKIDLII--------- 192
+ GI + S ++ +L+VL+ GGA+ P+DP++P +RI ++S S+ +++
Sbjct: 1552 LIGIMVDRSPGMVVGMLAVLKAGGAYTPIDPSYPPERIQYMLSDSQAPILLTQRHLQELA 1611
Query: 193 -YSGSSFCEDGYHLTDGFRW-LEQIRSCSTFSFTMEENSIPEHNSAVDLVFPCERLLNRF 252
Y G D + G L+ + ++ + + + V + + + N
Sbjct: 1612 AYQGEIIDVDEEAIYTGADTNLDNVAGKDDLAYVIYTSGSTGNPKGV--MISHQAICNHM 1671
Query: 253 QWMQELFPSSGEELLLFKTSISFIDHIQEFLSAILTSSALIIPPMKELKEKLYSVVNFIQ 312
WM+E FP + E+ +L KT SF + EF ++T L++ ++ Y + I+
Sbjct: 1672 LWMRETFPLTTEDAVLQKTPFSFDASVWEFYLPLITGGQLVLAKPDGHRDIAY-MTRLIR 1731
Query: 313 AYSISKLTAVPSLMRALL--PALQRLCVMQNRCSLRLLILSGEILPIQLWNALVKLLPET 372
I+ L VPSL+ ++ P SL+ + GE L ALV ET
Sbjct: 1732 DEKITTLQMVPSLLDLVMTDPGWSACT------SLQRVFCGGEALT----PALVSRFYET 1791
Query: 373 T---VLNLYGSTEVSGDCTYFDCKRMPMILGTEEISTVPIGVPISHCDVVVVGDNDALNQ 432
++NLYG TE + D TY+ C R +E S +PIG PI + + VV ++ L
Sbjct: 1792 QQAQLINLYGPTETTIDATYWPCPRQ------QEYSAIPIGKPIDNVRLYVVNASNQLQP 1851
Query: 433 ----GELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGGSLNANCDQIYIRMGDFVRQL 492
GEL + G + GY+ F GG++ R GD VR L
Sbjct: 1852 VGVAGELCIAGDGLARGYWQREEL--TKASFVDNPFEPGGTM--------YRTGDMVRYL 1911
Query: 493 QSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDAAVVSRRSDRELEYLVAFLVL 552
G + +LGR D +K+ G RI L EIE L +H V V++R+ + L A++V
Sbjct: 1912 PDGHIEYLGRIDHQVKIRGHRIELGEIEATLLQHEAVKAVVVMARQDGKGQNSLYAYVV- 1971
Query: 553 KDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSSSGKVDYEILTHSRPLWEHEH 612
+ D+ + +R++L + M+P++F F++ +P+S++GKVD + L +P
Sbjct: 1972 ---AEQDIQTAELRTYLSATLPAYMVPSAFVFLEQLPLSANGKVDRKAL--PQPEDAAAS 2031
Query: 613 ETIDETWANDYMQVIKKAFSDALMIKEISSDDDFFTMGGNSITAAHV------SYRLGVD 660
+ N++ + + L ++ I D FF +GG+S+ A HV S+++ V
Sbjct: 2032 AAVYVAPRNEWEAKLAAIWESVLGVEPIGVHDHFFELGGHSLKAMHVISLLQRSFQVDVP 2069
BLAST of Cp4.1LG12g00680 vs. Swiss-Prot
Match:
ACSF4_DANRE (Acyl-CoA synthetase family member 4 OS=Danio rerio GN=aasdh PE=3 SV=1)
HSP 1 Score: 177.9 bits (450), Expect = 5.8e-43
Identity = 91/282 (32.27%), Postives = 146/282 (51.77%), Query Frame = 1
Query: 804 SMKKLWQVHMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIEC 863
+++ LW+ CVDASP+++ + +FIGSHS + +D + WE L R+E
Sbjct: 789 ALRVLWRSDTGRCVDASPMLLVAPDRTTVFIGSHSHRLQALDLSRGEVIWERILGDRLES 848
Query: 864 STAIVGDFSQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDH 923
S AI V +GCY ++YFL+ S G+ WTF+T VKS P VDP+ L++ GS+D
Sbjct: 849 SAAISSCGGLVAIGCYDRQMYFLDVSCGDTVWTFETGDVVKSSPTVDPKTGLVFAGSHDG 908
Query: 924 NLYALDYVRHSCVYKLPC-GGSIYGSPAIDGVQHRLYVASTSGRTSAL------TLWHYD 983
++YAL+ + +C ++ C GG+++ SP + +LY +S G L LW Y
Sbjct: 909 HVYALNPLTKTCTWQHYCGGGAVFSSPCVHLSPRQLYCSSLGGHLHCLNPDSGKVLWKYS 968
Query: 984 LEAPVFGSLAIDPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVV---- 1043
AP F S +V VNGH++ + +G+ W T GP+F+ CISS+
Sbjct: 969 SSAPFFSSPHCS--DSSVFIGSVNGHIIGISHSGNTLWDFSTDGPVFSSPCISSLTLLTN 1028
Query: 1044 --------------PSQVLIC-SRNGSIYSFELKSGDLVWEY 1060
P+ ++ C S +G +Y ++G L+W++
Sbjct: 1029 QPPSTTPSSSVTTSPNHIVTCGSHDGHVYCLNAQNGSLLWQF 1068
BLAST of Cp4.1LG12g00680 vs. Swiss-Prot
Match:
LGRC_BREPA (Linear gramicidin synthase subunit C OS=Brevibacillus parabrevis GN=lgrC PE=3 SV=1)
HSP 1 Score: 172.9 bits (437), Expect = 1.9e-41
Identity = 159/597 (26.63%), Postives = 296/597 (49.58%), Query Frame = 1
Query: 85 RLLAILRDPQLIAPTAPHRANDQLVKTCPVANELSEASVELDSSNVPKIFGIYMPPSVEY 144
R+ A+ D QL RAN +AN L + VE + + G+ + S++
Sbjct: 484 RIAAVAGDQQLTYAELEARANQ-------LANYLQKQGVEAGT-----LVGLCVDRSLDM 543
Query: 145 IIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKIDLII---YSGSSFC----EDGYHLT 204
+I +L++L+ GGA++P+DPA+P++R+ +++ +KI +++ + G + Y
Sbjct: 544 LIGLLAILKAGGAYVPIDPAYPEERLAFMLADAKISILLTQKHLGKQWKGRKRRTVYLDR 603
Query: 205 DGFRWLEQIRSCSTFSFTMEENSIPEHNSAVDL----VFPCERLLNRF-QWMQELFPSSG 264
D +W E+ T + + + S V R + R Q + P S
Sbjct: 604 DAKKWAEESPLAPDVDTTKDSLAYVIYTSGSTGTPKGVLAVHRGVVRLGQKTRTTSPISE 663
Query: 265 EELLLFKTSISFIDHIQEFLSAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVP 324
++ L +++SF E A+L + L++ P L S+ +A K+T +
Sbjct: 664 ADVFLQASTVSFDAATFEIWGALLNGAKLVLMP-----PDLPSLDELGEAIVQHKVTTL- 723
Query: 325 SLMRALLPALQRLCVMQNRCSLR---LLILSGEILPIQLWNALVKLLPETTVLNLYGSTE 384
L L + V N LR L++ G+++ + ++ L TV+N YG TE
Sbjct: 724 ----WLTAGLFSIMVDHNADYLRGVRQLLVGGDVVSVPHVRKVLAL-GGVTVINGYGPTE 783
Query: 385 VSGDCTYFDCKRMPMILGTEEISTVPIGVPISHCDVVVVGDNDAL----NQGELWVGGPC 444
+ T+ C P+ +E+I++ PIG PIS+ V V+ + GEL++GG
Sbjct: 784 ---NTTFTCC--YPVTELSEDITSFPIGRPISNTTVYVLDKHKQPVPYGAAGELYIGGDG 843
Query: 445 VCSGYYSDSTFHPLDGKISSQDFVHGGSLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDR 504
+ GY +++ +++++ FV ++Y R GD VR L +G + F+GR D
Sbjct: 844 LALGYLNNA-------ELTAERFVENPFDPQKGSRLY-RTGDLVRYLPNGTIEFIGRIDN 903
Query: 505 IIKVNGQRIALEEIENALREHPDVVDAAVVSRRSDRELEYLVAFLVLKDNKKSDVFRSTV 564
+K+ G RI L E+E AL HP+V + V++R +DR ++L A++ + + +V + +
Sbjct: 904 QVKIRGFRIELGEVEAALALHPEVSETVVMARENDRGEKHLTAYVTVAKDDAPEV--ADL 963
Query: 565 RSWLVEKVLLAMIPNSFFFIDSIPMSSSGKVDYEILTHSRPLWEHEHETIDETW-ANDYM 624
++WL K+ M+P+++ F+D++P++++GK+D L P W + ET T N
Sbjct: 964 QAWLKTKLPEYMVPSAYVFLDAMPLTANGKIDRRRL--PEPEWGNRSETKAYTEPRNQAE 1023
Query: 625 QVIKKAFSDALMIKEISSDDDFFTMGGNSITAAHVSYRL----GVD--MRWLYHYPS 656
++I +S L ++++ D+FF +GG+S+ A V RL GV+ +R ++ +P+
Sbjct: 1024 ELIASIWSQVLGVEKVGIHDNFFELGGHSLLATRVISRLREVFGVEQSVRSIFEHPT 1040
BLAST of Cp4.1LG12g00680 vs. Swiss-Prot
Match:
ACSF4_MOUSE (Acyl-CoA synthetase family member 4 OS=Mus musculus GN=Aasdh PE=2 SV=1)
HSP 1 Score: 165.2 bits (417), Expect = 3.9e-39
Identity = 93/268 (34.70%), Postives = 135/268 (50.37%), Query Frame = 1
Query: 804 SMKKLWQVHMESCVDASPLVVFK----HPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEG 863
++++ W+ CVDASPL+V P ++IGSHS VD + +WE L
Sbjct: 752 ALRERWRSDTGKCVDASPLLVRAAVQDKPSTTVYIGSHSHTVKAVDLSSGETRWEQLLGD 811
Query: 864 RIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCG 923
RIE S + + +VVGCY G +Y L+ ++G WTF T VKS P VDP LI+ G
Sbjct: 812 RIESSACVSKCGNFIVVGCYNGLVYVLKSNSGEKYWTFTTEDAVKSSPAVDPTTGLIYVG 871
Query: 924 SYDHNLYALDYVRHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSAL------TLW 983
S+D + YALD CV+KL C G+++ SP + H LY A+ G AL T+W
Sbjct: 872 SHDQHAYALDIYEKKCVWKLNCEGALFSSPCVSLSPHHLYCATLGGLLLALNPASGSTVW 931
Query: 984 HYDLEAPVFGSLAIDPLSGNVICCL--VNGHVVALDSNGSVSWKCKTGGPIFAGACISSV 1043
P+F S P C+ V+G ++ +G W+ GGPIF+ C+S+
Sbjct: 932 KRSCGKPLFSS----PRCYQQYICIGCVDGSLLCFTHSGEQVWRFAAGGPIFSSPCVSA- 991
Query: 1044 VPSQVLICSRNGSIYSFELKSGDLVWEY 1060
++ S + IY K G L W++
Sbjct: 992 AEQEIFFGSHDCFIYCCS-KEGHLRWKF 1013
BLAST of Cp4.1LG12g00680 vs. TrEMBL
Match:
A0A061F726_THECC (AMP-dependent synthetase and ligase family protein, putative isoform 1 OS=Theobroma cacao GN=TCM_025682 PE=4 SV=1)
HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 591/1145 (51.62%), Postives = 763/1145 (66.64%), Query Frame = 1
Query: 6 CCISHEFQRVALSHPEKIAVIHAS-------GGVQLCRQLHGGGGGGGGDENFFTERAIS 65
CCISHEF R A +PEKIAVIHAS GGVQ+ R+L GGG
Sbjct: 15 CCISHEFYRAASKNPEKIAVIHASSSSKPSAGGVQIDRELIGGGN--------------- 74
Query: 66 AFPSMYDGDQCFTYSQLLASVDSLSSRLLAILR---DPQLIAPTAPHRANDQLVKTCPVA 125
P +Y GDQCFT++ LLASVD LS RL +IL DP LI P D T PV
Sbjct: 75 --PPVYKGDQCFTFATLLASVDCLSFRLRSILEGADDPYLIKSQPP---GDNGKHTVPV- 134
Query: 126 NELSEASV----------ELDSSNVPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAW 185
+ SEAS+ EL+++ +PKI G++MPPSVEY+I+VLSVL+CG AF+PLDP+W
Sbjct: 135 -QTSEASLTFMQEVGRHTELENTYIPKIVGLFMPPSVEYVISVLSVLKCGEAFLPLDPSW 194
Query: 186 PKKRILSVVSTSKIDLIIYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHN 245
P+ RILS+V +S L+I GSSF + G D WL + SC F+MEE+S ++N
Sbjct: 195 PRDRILSIVDSSNAALVIACGSSFGKSGCEPLDQSHWLLECSSCPVLCFSMEESS-EKNN 254
Query: 246 SAVDLVFPCER----------------------------LLNRFQWMQELFPSSGEELLL 305
+PCE LLNRF WMQEL+P GEELLL
Sbjct: 255 IESSFGWPCENERKRLFCYLMYTSGSTGNPKGVCGTEQGLLNRFLWMQELYPMHGEELLL 314
Query: 306 FKTSISFIDHIQEFLSAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRA 365
FKTSISF+DH+QEFL+A LT+ L++PP+ EL++ ++S++ F++AYSI++LTAVPSLMR
Sbjct: 315 FKTSISFVDHLQEFLAASLTACTLVVPPLTELRQNVFSIIEFLEAYSINRLTAVPSLMRV 374
Query: 366 LLPALQRLCVMQNRCSLRLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYF 425
+LPA+Q SLRLL+LSGE+LP+ LWN L LLP+T+VLNLYGSTEVSGDC YF
Sbjct: 375 ILPAMQSQHDNLISSSLRLLVLSGEVLPLALWNMLSSLLPKTSVLNLYGSTEVSGDCMYF 434
Query: 426 DCKRMPMILGTEEISTVPIGVPISHCDVVVVGDNDALNQGELWVGGPCVCSGYYSDSTFH 485
DCKR+P IL + ++TVPIG+PIS C +V+ G+N N+GE++V G CV GY+S++
Sbjct: 435 DCKRLPSILEMQTLTTVPIGLPISKCSIVLNGENSNPNEGEIYVRGLCVSIGYFSENAII 494
Query: 486 PLDGKISSQDFVHGGSLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALE 545
PL+ Q+ + S+ A Q+Y R GDF QL SGDLVFLGRKDR +KVNGQRIALE
Sbjct: 495 PLNNAKLHQNSLCKCSMEACGSQVYFRTGDFAHQLPSGDLVFLGRKDRTVKVNGQRIALE 554
Query: 546 EIENALREHPDVVDAAVVSRRSDRELEYLVAFLVLKDNKKS-DVFRSTVRSWLVEKVLLA 605
E+EN LR H DV+DAAV+S + E +VAF++L++ ++S ++F++++R+W++ K+ A
Sbjct: 555 EVENTLRGHNDVIDAAVISHKDQGEDALIVAFILLREKEESGEMFKTSIRNWMISKLPTA 614
Query: 606 MIPNSFFFIDSIPMSSSGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALM 665
M+P F F+ S+PMS+SGKVDY +L S H + I ++ MQVIKKAF +ALM
Sbjct: 615 MVPTHFVFVKSLPMSASGKVDYTVLVESILSKSHVQDEISNIGPSNLMQVIKKAFCEALM 674
Query: 666 IKEISSDDDFFTMGGNSITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGSDIDINRD 725
++++S DDDFF +GGNSI AAHVS+ LG+DMR LY + +PAKLL L+EKKGS
Sbjct: 675 VEDVSDDDDFFMIGGNSIAAAHVSHNLGIDMRLLYTFSTPAKLLITLVEKKGS------- 734
Query: 726 ADSRKNLKIDRWNKFSFDDSEFLTHFDINEGQNSGKRKQVHP-----NDGFSRAAIPRNN 785
N +I D++E + D +S + + P S RN+
Sbjct: 735 --KNTNFRIK-------DNAELIIQPDKGSAYSSVESETPDPLGSKLQRTLSWTLYERND 794
Query: 786 NSSI-SKHNKEVSDFSINLEDIGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCV 845
+ ++ SK K S+ L+ + G+ W+S SC+FSR NKV+ + N
Sbjct: 795 DQAVRSKRLKVDSNKYYILDPVHLFNGYPWNSASILKSCSFSRCNKVMRAGENEVNDTWQ 854
Query: 846 ETLSVKSPRGENGSMKKLWQVHMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNAS 905
SV+ PR G M++LW+VHMESCVDASPL+VFK IYLF+GSHS KF+CV+A++ S
Sbjct: 855 VAQSVEVPRTRTGYMQELWKVHMESCVDASPLIVFKDSDIYLFVGSHSHKFLCVNAQSGS 914
Query: 906 LQWEIRLEGRIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVD 965
+QWE RL+GR+E S AIVGDFSQVVVGCY G +YFLEF GNI WTF T GEVK QP++D
Sbjct: 915 IQWETRLQGRVEGSAAIVGDFSQVVVGCYDGNLYFLEFLNGNICWTFHTSGEVKCQPIMD 974
Query: 966 PERNLIWCGSYDHNLYALDYVRHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSAL 1025
R LIWCGS+D NLYALDY CV KLPCGGSI+GSPAID V H LY+ASTSGR +A+
Sbjct: 975 NHRGLIWCGSHDRNLYALDYRNRCCVCKLPCGGSIFGSPAIDEVHHALYMASTSGRVTAI 1034
Query: 1026 --------TLWHYDLEAPVFGSLAIDPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPI 1085
TLW Y+LE PVFGSL+I P G VICCLV+GHVVALDS+GS+ WK +TGGPI
Sbjct: 1035 SIKELPFCTLWSYELEVPVFGSLSISPRHGYVICCLVDGHVVALDSSGSIVWKRRTGGPI 1094
Query: 1086 FAGACISSVVPSQVLICSRNGSIYSFELKSGDLVWEYNIGNPITASACVDEQLQLVPETS 1088
FAGACIS +PSQVLICSRNGS+YSFE++ G+L+WE N+G+PITASA VDE LQL+ +
Sbjct: 1095 FAGACISYALPSQVLICSRNGSVYSFEMEKGELLWEINVGDPITASAYVDENLQLISNPT 1120
BLAST of Cp4.1LG12g00680 vs. TrEMBL
Match:
B9IPY7_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0019s06060g PE=4 SV=2)
HSP 1 Score: 1074.7 bits (2778), Expect = 1.2e-310
Identity = 581/1127 (51.55%), Postives = 748/1127 (66.37%), Query Frame = 1
Query: 6 CCISHEFQRVALSHPEKIAVIHASGGVQLCRQLHGGGGGGGGDENFFTERAISAFPSMYD 65
CC+SH F + A +P K+AVI+A+ G E S P +Y+
Sbjct: 26 CCLSHLFLKAAAQNPPKVAVIYAAPSSSSSSS--PAAASSGPQTQISRELITSTTPPIYE 85
Query: 66 GDQCFTYSQLLASVDSLSSRLLAILR---DPQLIAPTAPHRANDQLVKTCPVANELSEAS 125
GDQCFT++ + +SVDSLSSRL +IL DP LI P +P +N +
Sbjct: 86 GDQCFTFANVFSSVDSLSSRLRSILDGADDPHLIKPQSPPGKG---------SNNPGKNQ 145
Query: 126 VELDSSNVPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKIDLI 185
E S+ PKI GIYMPPSVEYII+V S+LRCG AF+P+DP+WP+ R+LS+V+++ LI
Sbjct: 146 AETASAYNPKIVGIYMPPSVEYIISVFSILRCGEAFLPIDPSWPRDRVLSIVASANAALI 205
Query: 186 IYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHNSAVDLVFPCER------ 245
I S SSF + G + WL C F+ME++ S +L +PCE
Sbjct: 206 ITSRSSFGKGGNKDINEADWLVDRSGCRVLCFSMEDSECSGGPS--ELAWPCENEKERLF 265
Query: 246 ----------------------LLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFLSA 305
LLNRF WMQEL+P GEE LLFKTSISFIDH+QEFLSA
Sbjct: 266 CYLMYTSGSTGKPKGVCGTEQGLLNRFWWMQELYPLHGEEALLFKTSISFIDHLQEFLSA 325
Query: 306 ILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRCSL 365
+LT+ L+IPP ELKE +S+VN +QAYSI++LTAVPSLMRA+LP LQR MQ + SL
Sbjct: 326 MLTTCTLVIPPFHELKEYPFSLVNVLQAYSINRLTAVPSLMRAILPVLQRQHSMQIQTSL 385
Query: 366 RLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEISTV 425
+LL+LSGE+ + LW+AL LLP TT+LNLYG+TEVSGDCTYFDCKR+P IL TE ++++
Sbjct: 386 KLLVLSGEVFSLSLWDALSTLLPRTTILNLYGTTEVSGDCTYFDCKRLPAILETEALTSI 445
Query: 426 PIGVPISHCDVVVVGDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGGSL 485
PIG+PIS+CDV ++ ++D N+GE++VGG CV +GYYS+ST D + S+
Sbjct: 446 PIGLPISNCDVALICESDTSNEGEIYVGGLCVSNGYYSESTVTSFISANPHMDNICNSSV 505
Query: 486 NANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDAAV 545
+ Q Y R GDF ++LQ+GDLVFLGR DR +K+NGQRI LEEIEN LR HPDV DAAV
Sbjct: 506 DNWGCQAYYRTGDFAQRLQNGDLVFLGRTDRTVKINGQRIVLEEIENTLRGHPDVADAAV 565
Query: 546 VSRRSDRELEYLVAFLVLKDNKKSDVF--RSTVRSWLVEKVLLAMIPNSFFFIDSIPMSS 605
+SR EL +L A L+ K+ +KS+ F RS++R W+V+KV LAM+PN F +S+PMSS
Sbjct: 566 ISREGPGELLFLDAILLFKEREKSEDFFVRSSIRKWMVDKVPLAMVPNRFVITESLPMSS 625
Query: 606 SGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMIKEISSDDDFFTMGGN 665
+GKVDY +L S+ L H + I +D +Q+IKKAF D LM++E+S DDDFF MGGN
Sbjct: 626 TGKVDYALLARSKFLNLHVQDEIGNA-TSDLLQIIKKAFCDGLMVEEVSCDDDFFAMGGN 685
Query: 666 SITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGS-DIDINRDADSRKNLKIDRWNKF 725
SI+AAHVSY LG++MR LY++P+P+KL ALLEKK S +++ DA+S+ K D
Sbjct: 686 SISAAHVSYNLGINMRLLYNFPTPSKLHAALLEKKESYCMEVRVDANSQLKPKKDS---- 745
Query: 726 SFDDSEFLTH--FDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISKHNKEVSDFSINL 785
D + + + G S K+ +P+ ++++ SK KE D SI+
Sbjct: 746 LVSDMAYSPNPTSPVVPGLKSMKQPSKNPHQN-------NDDHTVASKRFKEDLDISISS 805
Query: 786 EDIGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKSPR-GENGSMKKL 845
+ G S + S+ C+FSR N V+Y+ K PR G+ SM +L
Sbjct: 806 ACVKPSDGQPLSSSI-SMLCSFSRCNTVIYDENCRSRKSHQINQLAKVPRNGKGSSMHEL 865
Query: 846 WQVHMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIECSTAIV 905
W+V+MESCVDASPLVV K +YLFIGSHS KFVCV+A + S+QWE++LEGRIE S AIV
Sbjct: 866 WKVYMESCVDASPLVVVKQQDVYLFIGSHSHKFVCVNALSGSIQWEVKLEGRIESSAAIV 925
Query: 906 GDFSQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDHNLYAL 965
GDFSQVVVGCY GKIYFL+F G+I WTFQTCGEVK QPVVD R LIWCGS+DHNLYAL
Sbjct: 926 GDFSQVVVGCYSGKIYFLDFLDGSICWTFQTCGEVKCQPVVDIHRQLIWCGSHDHNLYAL 985
Query: 966 DYVRHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSAL--------TLWHYDLEAP 1025
DY H C+YKL C GSIYGSPAID V + LYVASTSG +A+ TLW ++L+ P
Sbjct: 986 DYRNHCCIYKLSCDGSIYGSPAIDEVHNTLYVASTSGHVTAISIKALPFNTLWEHELKVP 1045
Query: 1026 VFGSLAIDPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVVPSQVLICS 1085
VFGSL++ P SGNVICCLV+G++V LD GS+ W+C TGGP+FAGACIS V+PSQVLICS
Sbjct: 1046 VFGSLSLCPSSGNVICCLVDGNIVVLDFCGSIIWRCGTGGPVFAGACISCVLPSQVLICS 1105
Query: 1086 RNGSIYSFELKSGDLVWEYNIGNPITASACVDEQLQLVPETSTSSDR 1088
RNG +YSFE+++GDL+W+ PITASA VDE LQL+ + SDR
Sbjct: 1106 RNGRVYSFEMETGDLLWD-----PITASAYVDEHLQLLSDPCLLSDR 1121
BLAST of Cp4.1LG12g00680 vs. TrEMBL
Match:
G7LCR6_MEDTR (AMP-dependent synthetase and ligase family protein OS=Medicago truncatula GN=MTR_8g035620 PE=4 SV=2)
HSP 1 Score: 1056.2 bits (2730), Expect = 2.7e-305
Identity = 565/1129 (50.04%), Postives = 738/1129 (65.37%), Query Frame = 1
Query: 6 CCISHEFQRVALSHPEKIAVIHASGGVQLCRQLHGGGGGGGGDENFFTERAISAFPSMYD 65
CCISHEF + A ++P KIAVIHASG L RQ +R S P Y
Sbjct: 8 CCISHEFFQTATANPNKIAVIHASGVANLSRQNSTSPNFNQDFTTLLQQRVDSTSPPFYH 67
Query: 66 GDQCFTYSQLLASVDSLSSRLLAILR---DPQLIAPTAP-----HRANDQLVKTCPVAN- 125
GD+ FTYSQLL S+ SLSSRL +IL DP LI + HR + K+ + N
Sbjct: 68 GDRSFTYSQLLDSIRSLSSRLSSILHGAHDPHLITAKSQGNDGVHREEGTVQKSESLKNV 127
Query: 126 -ELSEASVELDSSNVPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVS 185
+E++V PKI GIYMPPSVEYIIAVLSVLRCG AF+PLDP WP +RILSV S
Sbjct: 128 KPRAESNVNSIEEYKPKIVGIYMPPSVEYIIAVLSVLRCGEAFLPLDPFWPNERILSVAS 187
Query: 186 TSKIDLIIYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHNSAVDLVFPC- 245
+S +DLII S SSF + D WL ++ SC +++EEN + E +S+ D C
Sbjct: 188 SSNVDLIIGSQSSFSKSNLDRLDESHWLVKLISCPILRYSIEEN-LQECSSSTDFACHCS 247
Query: 246 ---------------------------ERLLNRFQWMQELFPSSGEELLLFKTSISFIDH 305
+ L NRF WMQ ++P +G+ELLLFK+SISFIDH
Sbjct: 248 NEKKRSFCYLMYTSGSSGKPKGVCGTEQGLSNRFLWMQGMYPLTGQELLLFKSSISFIDH 307
Query: 306 IQEFLSAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCV 365
+QEFLS+ILT+ LIIPP ELKE +YS+++F+QAYS+++LTAVPSL+R +LP LQ
Sbjct: 308 LQEFLSSILTACVLIIPPFSELKENVYSIIDFLQAYSVNRLTAVPSLIRTILPVLQTHTD 367
Query: 366 MQNRCSLRLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILG 425
++ SL+LL+LSGE P LW L +LP+T++LNLYGSTEVSGDCTYFDCKR+P++L
Sbjct: 368 LRIESSLKLLVLSGETFPYTLWETLSTILPKTSILNLYGSTEVSGDCTYFDCKRIPLVLK 427
Query: 426 TEEISTVPIGVPISHCDVVVVGDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQD 485
E +++VPIG+PI++C+VV++G+N A N+GEL+VGG C+ GYY +S Q+
Sbjct: 428 EEMLTSVPIGLPITNCNVVLIGENGAPNEGELYVGGSCIFRGYYDESDIMSEGFVKLPQN 487
Query: 486 FVHGGSLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHP 545
+ S++ ++Y R GD V+QL SGD +FLGRKDRI+KV+GQRI+LEE+EN LREHP
Sbjct: 488 YGCENSVDVFQSELYFRTGDLVKQLPSGDFIFLGRKDRIVKVHGQRISLEEVENLLREHP 547
Query: 546 DVVDAAVVSRRSDRELEYLVAFLVLKDNKK-SDVFRSTVRSWLVEKVLLAMIPNSFFFID 605
++ DAAVV R EL ++ AF++LKD ++ ++ +RSW++ K+ +PN F F +
Sbjct: 548 NINDAAVVCRNLQAELVFIEAFIILKDKQQLGELLVPAIRSWMINKLPSVWLPNRFIFTE 607
Query: 606 SIPMSSSGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMIKEISSDDDF 665
S P+SSSGKV+YE+L S L + + + ++ +Q+IKK F DAL+++++ +DDDF
Sbjct: 608 SFPISSSGKVNYELLVSSALLTKSVKDKVGNISCSNLLQLIKKIFHDALLVEKLCNDDDF 667
Query: 666 FTMGGNSITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGSDIDINRDADSRKNLKID 725
F MGGNS++AAHV++ LG+D+R+LY+YPSP KL ALL K+GS ++ D+ L D
Sbjct: 668 FIMGGNSLSAAHVAHNLGIDLRFLYYYPSPFKLCMALLHKRGS-CSLHNRLDNCLQLDTD 727
Query: 726 RWNKFSFDDSEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISKHNKEVSDFS 785
N D S LT + +V F R + R + ++ E
Sbjct: 728 IQNN---DFSSNLTESSFPLESRMIPKDKVDVLFPFKR--LKRGSTDVVTSGGDEPFP-- 787
Query: 786 INLEDIGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKSPRGENGSMK 845
W SL S +FSR NKV+Y+ + T S PRG G MK
Sbjct: 788 -------------WHSLAIFSSSSFSRCNKVLYKGQTSVMDTHQTTWSSNVPRGSRGHMK 847
Query: 846 KLWQVHMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIECSTA 905
W+V+MESCVDASP+VV K +YLFIGSHS KF+C++ ++ S+QWEI+LEGRIEC+ A
Sbjct: 848 SFWKVYMESCVDASPMVVSKGSDLYLFIGSHSHKFLCINVRSGSMQWEIKLEGRIECTAA 907
Query: 906 IVGDFSQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDHNLY 965
IV DFSQ +VGCY GKIYFL+F G+I W FQT GEVKSQP+VD R LIWCGSYDH LY
Sbjct: 908 IVSDFSQAIVGCYMGKIYFLDFWNGHICWIFQTSGEVKSQPIVDTCRQLIWCGSYDHTLY 967
Query: 966 ALDYVRHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSALT--------LWHYDLE 1025
ALDY H CVYKL CGGSIYGSPAID V+ LYVAST GR +A++ LW +LE
Sbjct: 968 ALDYKNHCCVYKLSCGGSIYGSPAIDEVRGLLYVASTGGRITAVSISGSPFSILWLLELE 1027
Query: 1026 APVFGSLAIDPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVVPSQVLI 1085
PVFGSLA+ +G VICCLV+GHV+ALD NGS+ WK TGGPIFAG CI SV P +VL+
Sbjct: 1028 VPVFGSLAVTK-NGTVICCLVDGHVLALDPNGSIVWKKTTGGPIFAGPCIPSVNPHEVLV 1087
Query: 1086 CSRNGSIYSFELKSGDLVWEYNIGNPITASACVDEQLQLVPETSTSSDR 1088
C RNGS+YSF+L+ GDL+WEYN+G+PITASA VDE LQL + S +SDR
Sbjct: 1088 CCRNGSVYSFKLEKGDLIWEYNVGDPITASAYVDEHLQLEADASHTSDR 1113
BLAST of Cp4.1LG12g00680 vs. TrEMBL
Match:
A0A0B2P8T2_GLYSO (Putative acyl-activating enzyme 19 OS=Glycine soja GN=glysoja_036624 PE=4 SV=1)
HSP 1 Score: 1048.9 bits (2711), Expect = 4.3e-303
Identity = 570/1138 (50.09%), Postives = 746/1138 (65.55%), Query Frame = 1
Query: 6 CCISHEFQRVALSHPEKIAVIHASGGVQLCRQLHGGGGG----GGGDENFFTERAISAFP 65
CCISHEF R A ++P KIA IHASG L RQ H G +R S P
Sbjct: 13 CCISHEFFRTASANPNKIAAIHASGVAHLSRQFHRENSTTPNFDGDLATLLEKRVESTSP 72
Query: 66 SMYDGDQCFTYSQLLASVDSLSSRLLAILR---DPQLIAPTAPHRANDQLVKTCPVANEL 125
+Y GD+ FTYS++ +V SLS RL +IL DP LI TA R ND + C
Sbjct: 73 PLYHGDRSFTYSRVSNAVRSLSFRLRSILLGADDPHLI--TAQSRGNDSV--NCEEGTVQ 132
Query: 126 SEASVE--LDSSNV---------PKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPK 185
+ S+E + S V PKI GIYMPPSVEY++AVLSVLRCG AF+PLDP WP
Sbjct: 133 APESLETVMPSEGVMNESSREYRPKIVGIYMPPSVEYVVAVLSVLRCGEAFLPLDPFWPN 192
Query: 186 KRILSVVSTSKIDLIIYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHNSA 245
+RILSV +S +DLII S SSF + D WL + +C +++++EN I +
Sbjct: 193 ERILSVAYSSNVDLIIGSQSSFGKSNLDKLDESHWLVKSINCPVLNYSIDEN-IQVCSGP 252
Query: 246 VDLVFPC----------------------------ERLLNRFQWMQELFPSSGEELLLFK 305
DL +PC + L NRF WMQ ++P +G+ELLLF
Sbjct: 253 TDLTWPCANEKRRSFSYLMYTSGSTGKPKGVCGTEQGLSNRFLWMQGMYPLNGQELLLFN 312
Query: 306 TSISFIDHIQEFLSAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRALL 365
+S+SFIDH+QEFLSAILT+ L+IPP ELKE +YS+++F+QAY +++LT VPSLMR +L
Sbjct: 313 SSVSFIDHLQEFLSAILTACVLVIPPFNELKENIYSIIDFLQAYFVNRLTTVPSLMRTIL 372
Query: 366 PALQRLCVMQNRCSLRLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDC 425
P LQ M SL+LL+LSGE P+ LW L +LP+T++LNLYGSTEVSGDCTYFDC
Sbjct: 373 PGLQTHANMLVENSLKLLVLSGETFPLTLWEMLSTILPKTSILNLYGSTEVSGDCTYFDC 432
Query: 426 KRMPMILGTEEISTVPIGVPISHCDV-VVVGDNDALNQGELWVGGPCVCSGYYSDSTFHP 485
KRMP+IL E++++VPIG+PI++CDV +++ +N A N+GEL+VGG C+ YY++
Sbjct: 433 KRMPLILKEEKLTSVPIGLPITNCDVMMLLNENGASNEGELYVGGSCIFRDYYNEP---- 492
Query: 486 LDGKISSQDFVHGGSLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEE 545
I S F A Q+Y R GD V+QL SGD VFLGRKDRIIK+NGQRIALEE
Sbjct: 493 ---NIMSDAFAKLPRSYACQGQLYFRTGDLVKQLPSGDFVFLGRKDRIIKINGQRIALEE 552
Query: 546 IENALREHPDVVDAAVVSRRSDRELEYLVAFLVLKDNKKS-DVFRSTVRSWLVEKVLLAM 605
+E LREHP + DAAVV R ++ EL L AF++LK ++S ++ +RSW++ K+ +
Sbjct: 553 VEELLREHPYINDAAVVCRNNEAELVLLEAFIILKKKERSGELLIPAIRSWMINKLPSIV 612
Query: 606 IPNSFFFIDSIPMSSSGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMI 665
IPN FFF++S P+S SGKV+YE+L S L ++ + + ++ +Q+IKKAF DALM+
Sbjct: 613 IPNRFFFMESFPVSPSGKVNYELLVGSALLTKNVKDKVSNIDCSNLLQLIKKAFHDALMV 672
Query: 666 KEISSDDDFFTMGGNSITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGSDIDINRDA 725
+++ +DDDFF MGGNS++AAHV+Y LG+DM++LY+YP+P KL ALL+KKGS ++
Sbjct: 673 EKVCNDDDFFMMGGNSLSAAHVAYGLGIDMKFLYYYPTPFKLCMALLQKKGS-CSLHNRL 732
Query: 726 DSRKNLKIDRWNKFSFDDSEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISK 785
D + + DR + H +N +NS + SR + N++ S
Sbjct: 733 DCCRQINTDRQD----------NHISMNHAENSSPLE--------SRMILKDNDHDSFP- 792
Query: 786 HNKEVSDFSINLEDIGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKS 845
+K + I++ G + L S S FSR NKV+Y+ K T S
Sbjct: 793 -SKRLKRGLIDVTSWGDESFPWYSPSLLSFS--FSRCNKVLYKGKQAVIDTNQTTWSANV 852
Query: 846 PRGENGSMKKLWQVHMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRL 905
PRG G M W+V++ESCVDASP++VFK IYLFIGSHS KF+C++A++ S+QWEI+L
Sbjct: 853 PRGSRGHMNNFWKVYLESCVDASPILVFKGTDIYLFIGSHSHKFLCINARSGSVQWEIKL 912
Query: 906 EGRIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIW 965
+GRIEC+ AIV DFSQVVVGCY GKI+FL+F G I W FQT GEVK+QPVVD R LIW
Sbjct: 913 KGRIECTAAIVSDFSQVVVGCYMGKIHFLDFLNGRICWIFQTSGEVKAQPVVDTCRQLIW 972
Query: 966 CGSYDHNLYALDYVRHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSALT------ 1025
CGS+DHNLYALDY +H CVYKL CGGSIYGSPAID V+ LYVAST GR +A++
Sbjct: 973 CGSHDHNLYALDYKKHCCVYKLSCGGSIYGSPAIDEVRGLLYVASTGGRITAISISASPF 1032
Query: 1026 --LWHYDLEAPVFGSLAIDPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACIS 1085
LW ++LE PVFGSLA+ +G VICCLV+GHV+ALD NGS+ WK T GPIFAG CI
Sbjct: 1033 TILWLHELEVPVFGSLAV-AHNGTVICCLVDGHVLALDPNGSIVWKKTTDGPIFAGPCIP 1092
Query: 1086 SVVPSQVLICSRNGSIYSFELKSGDLVWEYNIGNPITASACVDEQLQLVPETSTSSDR 1088
SV+P +VL+CSR+G +YSF+L+ GDL+WEYN+G+PITASA VDE LQL + S SSDR
Sbjct: 1093 SVLPHEVLVCSRSGGVYSFKLEKGDLLWEYNVGDPITASAYVDEHLQLESDASHSSDR 1114
BLAST of Cp4.1LG12g00680 vs. TrEMBL
Match:
K7KCC2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_03G021000 PE=4 SV=1)
HSP 1 Score: 1046.2 bits (2704), Expect = 2.8e-302
Identity = 569/1140 (49.91%), Postives = 743/1140 (65.18%), Query Frame = 1
Query: 6 CCISHEFQRVALSHPEKIAVIHASGGVQLCRQLHGGGGGG----GGDENFFTERAISAFP 65
CCISHEF R A ++P KIA IHASG L RQ H G +R S P
Sbjct: 13 CCISHEFFRTASANPNKIAAIHASGVAHLSRQFHRENSTAPNFDGDLATLLEKRVESTSP 72
Query: 66 SMYDGDQCFTYSQLLASVDSLSSRLLAILR---DPQLIAPTAPHRAN------------- 125
+Y GD+ FTYS++ +V SLS RL +IL DP LI T R N
Sbjct: 73 PLYHGDRSFTYSRVSNAVRSLSFRLRSILLGADDPHLI--TVQSRGNVSVNCEEGTVQTP 132
Query: 126 DQLVKTCPVANELSEASVELDSSNVPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAW 185
+ L P ++E+S E PKI GIYMPPSVEY++AVLSVLRCG AF+PLDP W
Sbjct: 133 ESLETVMPSEGVMNESSREYR----PKIVGIYMPPSVEYVVAVLSVLRCGEAFLPLDPIW 192
Query: 186 PKKRILSVVSTSKIDLIIYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHN 245
P +RILSV +S +DLII S SSF + D WL + SC +++++EN I +
Sbjct: 193 PNERILSVAYSSNVDLIIGSQSSFGKSNLDKLDESHWLVKSISCPVLNYSIDEN-IQVCS 252
Query: 246 SAVDLVFPC----------------------------ERLLNRFQWMQELFPSSGEELLL 305
DL +PC + L NRF WMQ ++P +G+ELLL
Sbjct: 253 GPTDLTWPCANEKRRSFSYLMYTSGSTGKPKGVCGTEQGLSNRFLWMQGMYPLNGQELLL 312
Query: 306 FKTSISFIDHIQEFLSAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRA 365
F +S+SFIDH+QEFLSAILT+ L+IPP ELKE +YS+++F+QAY +++LT VPSLMR
Sbjct: 313 FNSSVSFIDHLQEFLSAILTACVLVIPPFNELKENIYSIIDFLQAYFVNRLTTVPSLMRT 372
Query: 366 LLPALQRLCVMQNRCSLRLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYF 425
+LP LQ M SL+LL+LSGE P+ LW L +LP+T++LNLYGSTEVSGDCTYF
Sbjct: 373 ILPGLQTHANMLVENSLKLLVLSGETFPLTLWEMLSTILPKTSILNLYGSTEVSGDCTYF 432
Query: 426 DCKRMPMILGTEEISTVPIGVPISHCDV-VVVGDNDALNQGELWVGGPCVCSGYYSDSTF 485
DCKRMP+IL E++ +VPIG+PI++CDV +++ +N A N+GEL+VGG C+ YY++
Sbjct: 433 DCKRMPLILKEEKLFSVPIGLPITNCDVMMLLNENGASNEGELYVGGSCIFRDYYNE--- 492
Query: 486 HPLDGKISSQDFVHGGSLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIAL 545
I S F A Q+Y R GD V+QL SGD VFLGRKDRIIK+NGQRIAL
Sbjct: 493 ---PNNIMSDAFAKLPRSYACQGQLYFRTGDLVKQLPSGDFVFLGRKDRIIKINGQRIAL 552
Query: 546 EEIENALREHPDVVDAAVVSRRSDRELEYLVAFLVLKDNKKS-DVFRSTVRSWLVEKVLL 605
EE+E LREHP + DAAVV R ++ EL L AF++LK ++S ++ +RSW++ K+
Sbjct: 553 EEVEELLREHPYINDAAVVCRNNEAELVLLEAFIILKKKERSGELLIPAIRSWMINKLPS 612
Query: 606 AMIPNSFFFIDSIPMSSSGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDAL 665
++PN FFF++S P+S SGKV+YE+L S L ++ + + ++ +Q+IKKAF DAL
Sbjct: 613 IVLPNRFFFMESFPVSPSGKVNYELLVGSALLTKNVKDKVSNIDCSNLLQLIKKAFHDAL 672
Query: 666 MIKEISSDDDFFTMGGNSITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGSDIDINR 725
M++++ +DDDFF MGGNS++AAHV+Y LG+DM++LY+YP+P KL ALL+KKGS ++
Sbjct: 673 MVEKVCNDDDFFMMGGNSLSAAHVAYGLGIDMKFLYYYPTPFKLCMALLQKKGS-CSLHN 732
Query: 726 DADSRKNLKIDRWNKFSFDDSEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSI 785
D + + DR + H +N +NS + SR + N++ S
Sbjct: 733 RLDCCRQINTDRQD----------NHISMNHAENSRPLE--------SRMILKDNDHDSF 792
Query: 786 SKHNKEVSDFSINLEDIGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSV 845
+K + I++ G + L S S FSR NKV+Y+ K T S
Sbjct: 793 P--SKRLKRGLIDVTSWGDESFPWYSPSLLSFS--FSRCNKVLYKGKQAVIDTNQTTWSA 852
Query: 846 KSPRGENGSMKKLWQVHMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEI 905
PRG G M W+V+MESCVDASP++VFK IYLFIGSHS KF+C++A++ S+QWEI
Sbjct: 853 NVPRGSRGHMNNFWKVYMESCVDASPILVFKGTDIYLFIGSHSHKFLCINARSGSVQWEI 912
Query: 906 RLEGRIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNL 965
+L+GRIEC+ AIV DFSQVVVGCY GKI+FL+F G I W FQT GEVK+QPVVD R L
Sbjct: 913 KLKGRIECTAAIVSDFSQVVVGCYMGKIHFLDFLNGRICWIFQTSGEVKAQPVVDTCRQL 972
Query: 966 IWCGSYDHNLYALDYVRHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSALT---- 1025
IWCGS+DHNLYALDY +H CVYKL CGGSIYGSPAID V+ LYVAST GR +A++
Sbjct: 973 IWCGSHDHNLYALDYKKHCCVYKLSCGGSIYGSPAIDEVRGLLYVASTGGRITAISISAS 1032
Query: 1026 ----LWHYDLEAPVFGSLAIDPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGAC 1085
LW ++LE PVFGSLA+ +G VICCLV+GHV+ALD NGS+ WK T GPIFAG C
Sbjct: 1033 PFTILWLHELEVPVFGSLAV-AHNGTVICCLVDGHVLALDPNGSIVWKKTTDGPIFAGPC 1092
Query: 1086 ISSVVPSQVLICSRNGSIYSFELKSGDLVWEYNIGNPITASACVDEQLQLVPETSTSSDR 1088
I SV+P +VL+CSR+G +YSF+L+ GDL+WEYN+G+PITASA VDE LQL + S SSDR
Sbjct: 1093 IPSVLPHEVLVCSRSGGVYSFKLEKGDLLWEYNVGDPITASAYVDEHLQLESDASHSSDR 1115
BLAST of Cp4.1LG12g00680 vs. TAIR10
Match:
AT5G35930.1 (AT5G35930.1 AMP-dependent synthetase and ligase family protein)
HSP 1 Score: 889.0 bits (2296), Expect = 2.9e-258
Identity = 479/1000 (47.90%), Postives = 630/1000 (63.00%), Query Frame = 1
Query: 130 VPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKIDLIIYSGSSF 189
+PK+ +YMPPSVEY+I+V SVLRCG AF+PLDP+WP++R+LS++S+S I L+I G S
Sbjct: 1 MPKVVALYMPPSVEYVISVFSVLRCGEAFLPLDPSWPRERVLSLISSSNISLVIACGLSS 60
Query: 190 CEDGYHLT---------------------DGFRW---LEQIRSCSTFSFTMEENSIPEHN 249
E + + F W E+ R +T P+
Sbjct: 61 VESHWLVERNVCPVLLFSMDEKLSVETGCSSFVWPCKKERQRKFCYLMYTSGSTGKPKGV 120
Query: 250 SAVDLVFPCERLLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFLSAILTSSALIIPP 309
+ + LLNRF WMQEL+P GE+ FKTS+ FIDHIQEFL AIL+S+AL+IPP
Sbjct: 121 CGTE-----QGLLNRFLWMQELYPVVGEQRFAFKTSVGFIDHIQEFLGAILSSTALVIPP 180
Query: 310 MKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQ-RLCVMQNRCSLRLLILSGEIL 369
LKE + S+++F++ YSIS+L AVPS++RA+LP LQ R + + L+L++LSGE
Sbjct: 181 FTLLKENMISIIDFLEEYSISRLLAVPSMIRAILPTLQHRGHNNKLQSCLKLVVLSGEPF 240
Query: 370 PIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEISTVPIGVPISHCD 429
P+ LW++L LLPET LNLYGSTEVSGDCTYFDC +P +L TEEI +VPIG IS+C
Sbjct: 241 PVSLWDSLHSLLPETCFLNLYGSTEVSGDCTYFDCSELPRLLKTEEIGSVPIGKSISNCK 300
Query: 430 VVVVGDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFV--HGGSL-----NAN 489
VV++GD D +GE+ V G C+ GY S I S+ +V H SL N
Sbjct: 301 VVLLGDEDKPYEGEICVSGLCLSQGYMHSS--------IESEGYVKLHNNSLCNHLTNDC 360
Query: 490 CDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDAAVVSR 549
Q+Y R GD+ RQL SGDL+F+GR+DR +K+NG+R+ALEEIE L +PD+ +A V+
Sbjct: 361 GSQLYYRTGDYGRQLSSGDLIFIGRRDRTVKLNGKRMALEEIETTLELNPDIAEAVVLLS 420
Query: 550 RSDRELEYLVAFLVL-KDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSSSGKV 609
R + EL L AF+VL K++ SD ++R+W+ K+ MIPN F ++ +P++SSGKV
Sbjct: 421 RDETELASLKAFVVLNKESNSSDGIIFSIRNWMGGKLPPVMIPNHFVLVEKLPLTSSGKV 480
Query: 610 DYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMIKEISSDDDFFTMGGNSITA 669
DYE L + + + N +Q IKKA DAL++KE+S DDDFF +GG+S+ A
Sbjct: 481 DYEALARLKCPTTGAQDMMQSNGTNSLLQNIKKAVCDALLVKEVSDDDDFFAIGGDSLAA 540
Query: 670 AHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGS-DIDINRDADSRKNLKIDRWNKFSFDD 729
AH+S+ LG+DMR +Y + SP++LL L EK+G D+ + + + KI+ N
Sbjct: 541 AHLSHSLGIDMRLIYQFRSPSRLLIYLSEKEGKLREDMQHNTTQKLDHKIESQNGNGLVS 600
Query: 730 SEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISKHNKEVSDFSINLEDIGQV 789
H + G K Q N+ R I S K KE
Sbjct: 601 RTVPLHSGVTSGPTPSKL-QCEKNNSPKRLKIDYEKFSP--KRMKE-------------- 660
Query: 790 GGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKSPRGENGSMKKLWQVHMES 849
LWDS + + CAFSR NKV E S++ PR + SM+++W+VHMES
Sbjct: 661 -NKLWDSGFSQIQCAFSRCNKVHSPESCSNEEANREYWSLEIPRNQMVSMQEIWKVHMES 720
Query: 850 CVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIECSTAIVGDFSQVV 909
CVDASPLVV K K YLFIGSHS+KF C+DAK+ S+ WE LEGRIE S +VGDFSQVV
Sbjct: 721 CVDASPLVVLKDSKTYLFIGSHSRKFSCIDAKSGSMYWETILEGRIEGSAMVVGDFSQVV 780
Query: 910 VGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDHNLYALDYVRHSC 969
+GCYKGK+YFL+FSTG++ W FQ CGE+K QPVVD LIWCGS+DH LYALDY C
Sbjct: 781 IGCYKGKLYFLDFSTGSLCWKFQACGEIKCQPVVDTSSQLIWCGSHDHTLYALDYRSQCC 840
Query: 970 VYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSAL--------TLWHYDLEAPVFGSLAI 1029
VYKL CGGSI+ SPAID LYVASTSGR A+ TLW ++LEAP+FGSL I
Sbjct: 841 VYKLQCGGSIFASPAIDEGHSSLYVASTSGRVIAVSIKDSPFHTLWLFELEAPIFGSLCI 900
Query: 1030 DPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVVPSQVLICSRNGSIYS 1088
P + NVICCLV+G V+A+ +G++ W+ +TGGPIFAG C+S V+PSQVL+C RNG +YS
Sbjct: 901 TPSTQNVICCLVDGQVIAMSPSGTIIWRYRTGGPIFAGPCMSHVLPSQVLVCCRNGCVYS 960
BLAST of Cp4.1LG12g00680 vs. TAIR10
Match:
AT1G20510.1 (AT1G20510.1 OPC-8:0 CoA ligase1)
HSP 1 Score: 80.9 bits (198), Expect = 5.5e-15
Identity = 80/364 (21.98%), Postives = 153/364 (42.03%), Query Frame = 1
Query: 216 MEENSIPEHNSAVDLVFPCERLLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFLSAI 275
M + I H + + +V + ++NRF GE+ + + I + F + +
Sbjct: 203 MSKGVISSHRNLIAMV---QTIVNRFG------SDDGEQRFICTVPMFHIYGLAAFATGL 262
Query: 276 LTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRCSLR 335
L + II K +++ +++ I Y + L VP ++ A++ ++ + S+
Sbjct: 263 LAYGSTIIVLSKF---EMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMH 322
Query: 336 LLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTE-EISTV 395
++ G L ++ + P +L YG TE +G D GT ++S
Sbjct: 323 TVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSAS 382
Query: 396 PIGVPISHCDVVVVGDNDALNQGELWVGGPCVCSGYYS--DSTFHPLDGKISSQDFVHGG 455
G + ++G GELW+ GP + GY+S ++T LD
Sbjct: 383 MEGRIVDPVTGQILGPKQT---GELWLKGPSIMKGYFSNEEATSSTLDS----------- 442
Query: 456 SLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDA 515
+ ++R GD + G + + R +IK G ++A E+E L HP++ DA
Sbjct: 443 -------EGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDA 502
Query: 516 AVVSRRSDRELEYLVAFLVLKDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSS 575
AV+ ++ +A++V K S + T+ ++ ++V F+ SIP +
Sbjct: 503 AVIPFPDKEVGQFPMAYVVRKTG--SSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNP 531
Query: 576 SGKV 577
SGK+
Sbjct: 563 SGKI 531
BLAST of Cp4.1LG12g00680 vs. TAIR10
Match:
AT4G19010.1 (AT4G19010.1 AMP-dependent synthetase and ligase family protein)
HSP 1 Score: 73.6 bits (179), Expect = 8.7e-13
Identity = 86/344 (25.00%), Postives = 149/344 (43.31%), Query Frame = 1
Query: 237 LLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFLSAILTSSALIIPPMKELKEKLYSV 296
L RF+ Q +P S + L + I + F+ +L+ + I+ MK V
Sbjct: 235 LFVRFEASQYEYPGSSN-VYLAALPLCHIYGLSLFVMGLLSLGSTIVV-MKRFDAS--DV 294
Query: 297 VNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRCSLRLLILSGEILPIQLWNALVKLL 356
VN I+ + I+ VP ++ AL + +C + SL+ + L + ++ L
Sbjct: 295 VNVIERFKITHFPVVPPMLMALTKKAKGVCGEVFK-SLKQVSSGAAPLSRKFIEDFLQTL 354
Query: 357 PETTVLNLYGSTEVSGDCTY-FDCKRMPMILGTEEISTVPIGVPISHCDVVVVGDNDAL- 416
P ++ YG TE + T F+ +++ S+V + P VV L
Sbjct: 355 PHVDLIQGYGMTESTAVGTRGFNSEKL------SRYSSVGLLAPNMQAKVVDWSSGSFLP 414
Query: 417 --NQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGGSLNANCDQIYIRMGDFVRQL 476
N+GELW+ GP V GY ++ K + V + ++R GD
Sbjct: 415 PGNRGELWIQGPGVMKGYLNNP-------KATQMSIV---------EDSWLRTGDIAYFD 474
Query: 477 QSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDAAVVSRRSDRELEYLVAFLVL 536
+ G L + R IIK G +IA ++E L HP ++DAAV + ++ E VAF+V
Sbjct: 475 EDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVR 534
Query: 537 KDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSSSGKV 577
+ +++ + V S++ +V ++SIP S +GK+
Sbjct: 535 R--QETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKI 549
BLAST of Cp4.1LG12g00680 vs. TAIR10
Match:
AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)
HSP 1 Score: 70.1 bits (170), Expect = 9.7e-12
Identity = 71/288 (24.65%), Postives = 123/288 (42.71%), Query Frame = 1
Query: 292 KLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRCSLRLLILSGEILPIQLWNA 351
+L V+ I+ + ++ L VP + AL + Q + + SL+ + L L
Sbjct: 269 ELELVLKNIEKFRVTHLWVVPPVFLAL--SKQSIVKKFDLSSLKYIGSGAAPLGKDLMEE 328
Query: 352 LVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEISTVPIGVPISHCDVVVV--G 411
+ +P ++ YG TE G + D + LG + + P +V V G
Sbjct: 329 CGRNIPNVLLMQGYGMTETCGIVSVEDPR-----LGKRNSGSAGMLAPGVEAQIVSVETG 388
Query: 412 DNDALNQ-GELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGGSLNANCDQIYIRMGDF 471
+ NQ GE+WV GP + GY ++ I + +VH G L Y
Sbjct: 389 KSQPPNQQGEIWVRGPNMMKGYLNNP--QATKETIDKKSWVHTGDLG------YFN---- 448
Query: 472 VRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDAAVVSRRSDRELEYLVA 531
+ G+L + R +IK G ++A E+E L HPD++DA V+ + E +A
Sbjct: 449 ----EDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIA 508
Query: 532 FLVLKDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSSSGKV 577
F+V N S + ++ ++ ++V FI +P S++GK+
Sbjct: 509 FVVRSPN--SSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKI 531
BLAST of Cp4.1LG12g00680 vs. TAIR10
Match:
AT1G20480.1 (AT1G20480.1 AMP-dependent synthetase and ligase family protein)
HSP 1 Score: 68.6 bits (166), Expect = 2.8e-11
Identity = 69/288 (23.96%), Postives = 123/288 (42.71%), Query Frame = 1
Query: 290 KEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRCSLRLLILSGEILPIQLW 349
K + +++ ++ + S L+ VP ++ A++ + + SL ++ G L ++
Sbjct: 286 KFDMAKLLSAVETHRSSYLSLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVT 345
Query: 350 NALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEISTVPIGVPISHCDVV-VV 409
V+ P+ +L YG TE + K G + + I D V+
Sbjct: 346 EKFVENYPKVKILQGYGLTESTAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVL 405
Query: 410 GDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGGSLNANCDQIYIRMGDF 469
G N GELW+ P V GY+ + I S+ ++ G L YI F
Sbjct: 406 GVNQT---GELWIRSPTVMKGYFKNK--EATASTIDSEGWLKTGDL------CYIDGDGF 465
Query: 470 VRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDAAVVSRRSDRELEYLVA 529
V + R +IK NG ++A E+E L HP++ DAAV+ + +Y +A
Sbjct: 466 V--------FVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIPDMKAGQYPMA 525
Query: 530 FLVLKDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSSSGKV 577
++V K S++ S + ++ ++V F+ SIP + SGK+
Sbjct: 526 YIVRKVG--SNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKI 552
BLAST of Cp4.1LG12g00680 vs. NCBI nr
Match:
gi|659114656|ref|XP_008457167.1| (PREDICTED: putative acyl-activating enzyme 19 isoform X1 [Cucumis melo])
HSP 1 Score: 1832.4 bits (4745), Expect = 0.0e+00
Identity = 922/1124 (82.03%), Postives = 984/1124 (87.54%), Query Frame = 1
Query: 1 MRQLPCCISHEFQRVALSHPEKIAVIHASGGVQLCRQLHGGGGGGGGDENFFTERAISAF 60
M+Q CCISHEFQRVALSHP KIAVIHASGGVQL RQLHGGG G D FF RA S F
Sbjct: 1 MKQPLCCISHEFQRVALSHPGKIAVIHASGGVQLFRQLHGGGVGEADD--FFQGRATSDF 60
Query: 61 PSMYDGDQCFTYSQLLASVDSLSSRLLAILRDPQLIAPTAPHRANDQLVKTCPVANELSE 120
P MY+GD+CFTYSQLLASVDSLSSRLLA LR PQL APTAP ANDQ KT PVANELSE
Sbjct: 61 PPMYEGDRCFTYSQLLASVDSLSSRLLATLRRPQLNAPTAPRPANDQPAKTSPVANELSE 120
Query: 121 ASVELDSSNVPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKID 180
AS EL++ N+PKIFGIYMPPSVEYII+VLSVLRCGGAFMPLDPAWPK+RILSVVS+SKID
Sbjct: 121 ASTELETCNIPKIFGIYMPPSVEYIISVLSVLRCGGAFMPLDPAWPKRRILSVVSSSKID 180
Query: 181 LIIYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHNSAVDLVFPCE----- 240
LIIYSGSSFCEDGYH+T+GFRWLE+I ST FTMEE+S+ EHNSAVDLVFPCE
Sbjct: 181 LIIYSGSSFCEDGYHVTEGFRWLEEISGYSTLCFTMEESSVREHNSAVDLVFPCEDEKAR 240
Query: 241 -----------------------RLLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFL 300
LLNRFQWMQE FPSS EELLLFKTSISFIDHIQEFL
Sbjct: 241 LFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQENFPSSREELLLFKTSISFIDHIQEFL 300
Query: 301 SAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRC 360
SAILTSS L+IPPMKELKE L SVVNFIQAYSI+KLTAVPSLMR LLPALQRLC + +C
Sbjct: 301 SAILTSSVLVIPPMKELKENLCSVVNFIQAYSINKLTAVPSLMRTLLPALQRLCGV--KC 360
Query: 361 SLRLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEIS 420
SLRLLILSGE LPIQLW+ALVKLLPETT+LNLYGSTEVSGDCTYFDCK+MPMIL T+ I+
Sbjct: 361 SLRLLILSGETLPIQLWDALVKLLPETTILNLYGSTEVSGDCTYFDCKKMPMILETDAIN 420
Query: 421 TVPIGVPISHCDVVVVGDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGG 480
T+PIGVPISHCDVVVVGDNDALNQGEL VGGPCVCSGYYSDS F PLDG SQDF+H G
Sbjct: 421 TIPIGVPISHCDVVVVGDNDALNQGELCVGGPCVCSGYYSDSIFLPLDGIKFSQDFIHEG 480
Query: 481 SLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDA 540
S N NC QIYIR GDFV+QL+SGDLVFLGRKDRIIKVNGQRI+LEEIE+ALREHPDVVDA
Sbjct: 481 SFNVNCSQIYIRTGDFVQQLRSGDLVFLGRKDRIIKVNGQRISLEEIEDALREHPDVVDA 540
Query: 541 AVVSRRSDRELEYLVAFLVLKDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSS 600
AVVSR+SD ELEYLVAFLVLKDN KS+VFRS VRSW+VEKV LAMIPNSFFFIDSIP ++
Sbjct: 541 AVVSRKSDWELEYLVAFLVLKDNMKSEVFRSPVRSWMVEKVSLAMIPNSFFFIDSIPKTT 600
Query: 601 SGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMIKEISSDDDFFTMGGN 660
SGKVDYEIL HSRPLWEH HE+IDETWAN+++Q+IKKAFSDALM++EISSDDDFFTMGGN
Sbjct: 601 SGKVDYEILMHSRPLWEHVHESIDETWANEFLQIIKKAFSDALMVEEISSDDDFFTMGGN 660
Query: 661 SITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGSD-IDINRDADSRKNLKIDRWNKF 720
SITAA VS+RLGVDMRWLYHYPSPAKLLT +LEKKG D I IN DADSR+NLK DRWNK+
Sbjct: 661 SITAALVSHRLGVDMRWLYHYPSPAKLLTVILEKKGLDIIGINGDADSRRNLKTDRWNKY 720
Query: 721 SFDDSEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISKHNKEVSDFSINLED 780
S +DSEFL HFD+ EG +SGKRKQV PN GFSRA +PRNNNS +SKH K VSD SINLED
Sbjct: 721 SLNDSEFLNHFDLKEGGSSGKRKQVQPNGGFSRAVVPRNNNSLLSKHCKVVSDHSINLED 780
Query: 781 IGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKSPRGENGSMKKLWQV 840
I QVGGHLW+S LTSVSCAFSR NKVVYEHKYIG++ C TLSVKSPRGE GSMKKLWQV
Sbjct: 781 ISQVGGHLWNSPLTSVSCAFSRCNKVVYEHKYIGDNECAGTLSVKSPRGEIGSMKKLWQV 840
Query: 841 HMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIECSTAIVGDF 900
HMESCVDASPL+VFKHP IYLFIGSHS KFVCVDAKNASL WEIRLEGRIECS AIVGDF
Sbjct: 841 HMESCVDASPLLVFKHPNIYLFIGSHSHKFVCVDAKNASLHWEIRLEGRIECSAAIVGDF 900
Query: 901 SQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDHNLYALDYV 960
SQVVVGCYKGKIYFLEFSTG IQWTFQT GEVKSQPVVDP+RNLIWCGSYDHNLYALDYV
Sbjct: 901 SQVVVGCYKGKIYFLEFSTGVIQWTFQTSGEVKSQPVVDPDRNLIWCGSYDHNLYALDYV 960
Query: 961 RHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSAL--------TLWHYDLEAPVFG 1020
RHSCVYKLPCGGS+YGSPAID VQHRLYVASTSGR SAL +LWHYDLEAPVFG
Sbjct: 961 RHSCVYKLPCGGSLYGSPAIDVVQHRLYVASTSGRISALLIKDFPFHSLWHYDLEAPVFG 1020
Query: 1021 SLAIDPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVVPSQVLICSRNG 1080
SLAIDP + NVICCLV+GHVVALDS GSVSWK KTGGPIFAG CIS+ +PSQVLICSRNG
Sbjct: 1021 SLAIDPFTRNVICCLVDGHVVALDSRGSVSWKSKTGGPIFAGPCISTSIPSQVLICSRNG 1080
Query: 1081 SIYSFELKSGDLVWEYNIGNPITASACVDEQLQLVPETSTSSDR 1088
SIYSFEL+SGDLVWEYNIGN ITASACVDE LQLVPETS SSDR
Sbjct: 1081 SIYSFELESGDLVWEYNIGNSITASACVDEHLQLVPETSISSDR 1120
BLAST of Cp4.1LG12g00680 vs. NCBI nr
Match:
gi|778668253|ref|XP_011649066.1| (PREDICTED: putative acyl-activating enzyme 19 isoform X1 [Cucumis sativus])
HSP 1 Score: 1812.7 bits (4694), Expect = 0.0e+00
Identity = 910/1124 (80.96%), Postives = 978/1124 (87.01%), Query Frame = 1
Query: 1 MRQLPCCISHEFQRVALSHPEKIAVIHASGGVQLCRQLHGGGGGGGGDENFFTERAISAF 60
M+Q CCISHEFQRVALSHP KIAVIHASGGVQL RQLHG GGGG D+ FF RA S+F
Sbjct: 1 MKQPLCCISHEFQRVALSHPGKIAVIHASGGVQLFRQLHGAGGGGEADD-FFQGRATSSF 60
Query: 61 PSMYDGDQCFTYSQLLASVDSLSSRLLAILRDPQLIAPTAPHRANDQLVKTCPVANELSE 120
P MY+ D+CFTYSQLLASVDSLSSRLLA +R PQL APTAP ANDQ KT PVA+ELSE
Sbjct: 61 PPMYEADRCFTYSQLLASVDSLSSRLLATVRGPQLNAPTAPRPANDQPAKTSPVASELSE 120
Query: 121 ASVELDSSNVPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKID 180
AS EL+SSN+PKIFGIYMPPSVEYII+VLSVLRCGGAFMPLDPAWPK+RILSVVS+ KID
Sbjct: 121 ASTELESSNIPKIFGIYMPPSVEYIISVLSVLRCGGAFMPLDPAWPKRRILSVVSSLKID 180
Query: 181 LIIYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHNSAVDLVFPCE----- 240
LIIYSGSSFC DGYH+T+GFRWLE+I ST F MEE+S+ EHNSAVDLVFPCE
Sbjct: 181 LIIYSGSSFCVDGYHVTEGFRWLEEISGYSTLCFNMEESSVREHNSAVDLVFPCEDEKAR 240
Query: 241 -----------------------RLLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFL 300
LLNRFQWMQE FPS+ EELLLFKTSISFIDHIQEFL
Sbjct: 241 LFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQENFPSTREELLLFKTSISFIDHIQEFL 300
Query: 301 SAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRC 360
SAILT+S L+ PPMKELKE L SVVNFIQAYSISKLTAVPSLMR LLPALQR C + +C
Sbjct: 301 SAILTASVLVTPPMKELKENLCSVVNFIQAYSISKLTAVPSLMRTLLPALQRFCGV--KC 360
Query: 361 SLRLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEIS 420
SLRLLILSGE LPI LW+ALVKLLPETT+LNLYGSTEVSGDCTYFDCK+MPMIL T+ I
Sbjct: 361 SLRLLILSGETLPILLWDALVKLLPETTILNLYGSTEVSGDCTYFDCKKMPMILETDAIK 420
Query: 421 TVPIGVPISHCDVVVVGDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGG 480
TVPIGVPISHCDVVVVGDNDALN GEL VGGPCVCSGYYSDS F PLDG SQDF+H G
Sbjct: 421 TVPIGVPISHCDVVVVGDNDALNLGELCVGGPCVCSGYYSDSVFLPLDGIKFSQDFIHEG 480
Query: 481 SLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDA 540
S N C QIYIR GDFV+QL+SGDLVFLGRKDRIIKVNGQRI+LEEIE+ALREHPDVVDA
Sbjct: 481 SFNVTCSQIYIRTGDFVQQLRSGDLVFLGRKDRIIKVNGQRISLEEIEDALREHPDVVDA 540
Query: 541 AVVSRRSDRELEYLVAFLVLKDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSS 600
AVVSR+SD ELEYLVAFLVLKDN+KS+VFRSTVRSW+VEKV LAMIPNSFFF DSIPM++
Sbjct: 541 AVVSRKSDWELEYLVAFLVLKDNEKSEVFRSTVRSWMVEKVPLAMIPNSFFFTDSIPMTT 600
Query: 601 SGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMIKEISSDDDFFTMGGN 660
SGKVDYEILTHSRPLWE HE+IDETWAN+++Q+IKKAFSDALM++EISS DDFFTMGGN
Sbjct: 601 SGKVDYEILTHSRPLWEQVHESIDETWANEFIQIIKKAFSDALMVEEISSGDDFFTMGGN 660
Query: 661 SITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGSD-IDINRDADSRKNLKIDRWNKF 720
SITAAHVS+RLG+DMRWLYHYPSPAKLLT +LEKKG D I IN DADSR+NLK DRWNK+
Sbjct: 661 SITAAHVSHRLGIDMRWLYHYPSPAKLLTVILEKKGLDIIRINEDADSRRNLKTDRWNKY 720
Query: 721 SFDDSEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISKHNKEVSDFSINLED 780
S DDSEFL HFD+ EG +SGKRKQV PN FSRA +PRNNNS +SKH K VSD SINLE+
Sbjct: 721 SLDDSEFLNHFDLKEGGSSGKRKQVQPNGDFSRAVVPRNNNSLLSKHYKAVSDCSINLEN 780
Query: 781 IGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKSPRGENGSMKKLWQV 840
I QVGGHLW S LTSVSCAFSR NKVVYE KYIG++ TL VKSPRGENGSMKKLWQV
Sbjct: 781 ISQVGGHLWHSPLTSVSCAFSRCNKVVYERKYIGDNKRAGTLLVKSPRGENGSMKKLWQV 840
Query: 841 HMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIECSTAIVGDF 900
HMESCVDASPL+VFKHP IYLFIGSHS KFVCVDAKNASL+WEIRLEGRIECS AIVGDF
Sbjct: 841 HMESCVDASPLLVFKHPNIYLFIGSHSHKFVCVDAKNASLRWEIRLEGRIECSAAIVGDF 900
Query: 901 SQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDHNLYALDYV 960
SQVVVGCYKG IYFLEFSTG I WTFQT GEVKSQPVVDP+RNLIWCGSYDHNLYALDYV
Sbjct: 901 SQVVVGCYKGNIYFLEFSTGVILWTFQTYGEVKSQPVVDPDRNLIWCGSYDHNLYALDYV 960
Query: 961 RHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSAL--------TLWHYDLEAPVFG 1020
RHSCVYKLPCGGS+YGSPAIDGVQHRLYVAST GR SAL +LWHYDLEAPVFG
Sbjct: 961 RHSCVYKLPCGGSLYGSPAIDGVQHRLYVASTGGRISALLIKDFPFNSLWHYDLEAPVFG 1020
Query: 1021 SLAIDPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVVPSQVLICSRNG 1080
SLAIDP++ NVICCLV+GHVVALDS+GSVSWK KTGGPIFAG CIS+ +PSQVLICSRNG
Sbjct: 1021 SLAIDPVTRNVICCLVDGHVVALDSSGSVSWKSKTGGPIFAGPCISTSIPSQVLICSRNG 1080
Query: 1081 SIYSFELKSGDLVWEYNIGNPITASACVDEQLQLVPETSTSSDR 1088
SIYSFEL+SGDLVWEYNIGNPITASACVDE LQLVPETS SSDR
Sbjct: 1081 SIYSFELESGDLVWEYNIGNPITASACVDEHLQLVPETSISSDR 1121
BLAST of Cp4.1LG12g00680 vs. NCBI nr
Match:
gi|659114658|ref|XP_008457168.1| (PREDICTED: putative acyl-activating enzyme 19 isoform X2 [Cucumis melo])
HSP 1 Score: 1799.6 bits (4660), Expect = 0.0e+00
Identity = 907/1116 (81.27%), Postives = 970/1116 (86.92%), Query Frame = 1
Query: 1 MRQLPCCISHEFQRVALSHPEKIAVIHASGGVQLCRQLHGGGGGGGGDENFFTERAISAF 60
M+Q CCISHEFQRVALSHP KIAVIHASGGVQL RQLHGGG G D FF RA S F
Sbjct: 1 MKQPLCCISHEFQRVALSHPGKIAVIHASGGVQLFRQLHGGGVGEADD--FFQGRATSDF 60
Query: 61 PSMYDGDQCFTYSQLLASVDSLSSRLLAILRDPQLIAPTAPHRANDQLVKTCPVANELSE 120
P MY+GD+CFTYSQLLASVDSLSSRLLA LR PQL APTAP ANDQ KT PVANELSE
Sbjct: 61 PPMYEGDRCFTYSQLLASVDSLSSRLLATLRRPQLNAPTAPRPANDQPAKTSPVANELSE 120
Query: 121 ASVELDSSNVPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKID 180
AS EL++ N+PKIFGIYMPPSVEYII+VLSVLRCGGAFMPLDPAWPK+RILSVVS+SKID
Sbjct: 121 ASTELETCNIPKIFGIYMPPSVEYIISVLSVLRCGGAFMPLDPAWPKRRILSVVSSSKID 180
Query: 181 LIIYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHNSAVDLVFPCE----- 240
LIIYSGSSFCEDGYH+T+GFRWLE+I ST FTMEE+S+ EHNSAVDLVFPCE
Sbjct: 181 LIIYSGSSFCEDGYHVTEGFRWLEEISGYSTLCFTMEESSVREHNSAVDLVFPCEDEKAR 240
Query: 241 -----------------------RLLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFL 300
LLNRFQWMQE FPSS EELLLFKTSISFIDHIQEFL
Sbjct: 241 LFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQENFPSSREELLLFKTSISFIDHIQEFL 300
Query: 301 SAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRC 360
SAILTSS L+IPPMKELKE L SVVNFIQAYSI+KLTAVPSLMR LLPALQRLC + +C
Sbjct: 301 SAILTSSVLVIPPMKELKENLCSVVNFIQAYSINKLTAVPSLMRTLLPALQRLCGV--KC 360
Query: 361 SLRLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEIS 420
SLRLLILSGE LPIQLW+ALVKLLPETT+LNLYGSTEVSGDCTYFDCK+MPMIL T+ I+
Sbjct: 361 SLRLLILSGETLPIQLWDALVKLLPETTILNLYGSTEVSGDCTYFDCKKMPMILETDAIN 420
Query: 421 TVPIGVPISHCDVVVVGDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGG 480
T+PIGVPISHCDVVVVGDNDALNQGEL VGGPCVCSGYYSDS F PLDG SQDF+H G
Sbjct: 421 TIPIGVPISHCDVVVVGDNDALNQGELCVGGPCVCSGYYSDSIFLPLDGIKFSQDFIHEG 480
Query: 481 SLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDA 540
S N NC QIYIR GDFV+QL+SGDLVFLGRKDRIIKVNGQRI+LEEIE+ALREHPDVVDA
Sbjct: 481 SFNVNCSQIYIRTGDFVQQLRSGDLVFLGRKDRIIKVNGQRISLEEIEDALREHPDVVDA 540
Query: 541 AVVSRRSDRELEYLVAFLVLKDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSS 600
AVVSR+SD ELEYLVAFLVLKDN KS+VFRS VRSW+VEKV LAMIPNSFFFIDSIP ++
Sbjct: 541 AVVSRKSDWELEYLVAFLVLKDNMKSEVFRSPVRSWMVEKVSLAMIPNSFFFIDSIPKTT 600
Query: 601 SGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMIKEISSDDDFFTMGGN 660
SGKVDYEIL HSRPLWEH HE+IDETWAN+++Q+IKKAFSDALM++EISSDDDFFTMGGN
Sbjct: 601 SGKVDYEILMHSRPLWEHVHESIDETWANEFLQIIKKAFSDALMVEEISSDDDFFTMGGN 660
Query: 661 SITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGSD-IDINRDADSRKNLKIDRWNKF 720
SITAA VS+RLGVDMRWLYHYPSPAKLLT +LEKKG D I IN DADSR+NLK DRWNK+
Sbjct: 661 SITAALVSHRLGVDMRWLYHYPSPAKLLTVILEKKGLDIIGINGDADSRRNLKTDRWNKY 720
Query: 721 SFDDSEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISKHNKEVSDFSINLED 780
S +DSEFL HFD+ EG +SGKRKQV PN GFSRA +PRNNNS +SKH K VSD SINLED
Sbjct: 721 SLNDSEFLNHFDLKEGGSSGKRKQVQPNGGFSRAVVPRNNNSLLSKHCKVVSDHSINLED 780
Query: 781 IGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKSPRGENGSMKKLWQV 840
I QVGGHLW+S LTSVSCAFSR NKVVYEHKYIG++ C TLSVKSPRGE GSMKKLWQV
Sbjct: 781 ISQVGGHLWNSPLTSVSCAFSRCNKVVYEHKYIGDNECAGTLSVKSPRGEIGSMKKLWQV 840
Query: 841 HMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIECSTAIVGDF 900
HMESCVDASPL+VFKHP IYLFIGSHS KFVCVDAKNASL WEIRLEGRIECS AIVGDF
Sbjct: 841 HMESCVDASPLLVFKHPNIYLFIGSHSHKFVCVDAKNASLHWEIRLEGRIECSAAIVGDF 900
Query: 901 SQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDHNLYALDYV 960
SQVVVGCYKGKIYFLEFSTG IQWTFQT GEVKSQPVVDP+RNLIWCGSYDHNLYALDYV
Sbjct: 901 SQVVVGCYKGKIYFLEFSTGVIQWTFQTSGEVKSQPVVDPDRNLIWCGSYDHNLYALDYV 960
Query: 961 RHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSALTLWHYDLEAPVFGSLAIDPLS 1020
RHSCVYKLPCGGS+YGSPAID V + +LWHYDLEAPVFGSLAIDP +
Sbjct: 961 RHSCVYKLPCGGSLYGSPAID-VDFPFH-----------SLWHYDLEAPVFGSLAIDPFT 1020
Query: 1021 GNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVVPSQVLICSRNGSIYSFELK 1080
NVICCLV+GHVVALDS GSVSWK KTGGPIFAG CIS+ +PSQVLICSRNGSIYSFEL+
Sbjct: 1021 RNVICCLVDGHVVALDSRGSVSWKSKTGGPIFAGPCISTSIPSQVLICSRNGSIYSFELE 1080
Query: 1081 SGDLVWEYNIGNPITASACVDEQLQLVPETSTSSDR 1088
SGDLVWEYNIGN ITASACVDE LQLVPETS SSDR
Sbjct: 1081 SGDLVWEYNIGNSITASACVDEHLQLVPETSISSDR 1100
BLAST of Cp4.1LG12g00680 vs. NCBI nr
Match:
gi|778668256|ref|XP_011649067.1| (PREDICTED: putative acyl-activating enzyme 19 isoform X2 [Cucumis sativus])
HSP 1 Score: 1779.2 bits (4607), Expect = 0.0e+00
Identity = 895/1116 (80.20%), Postives = 963/1116 (86.29%), Query Frame = 1
Query: 1 MRQLPCCISHEFQRVALSHPEKIAVIHASGGVQLCRQLHGGGGGGGGDENFFTERAISAF 60
M+Q CCISHEFQRVALSHP KIAVIHASGGVQL RQLHG GGGG D+ FF RA S+F
Sbjct: 1 MKQPLCCISHEFQRVALSHPGKIAVIHASGGVQLFRQLHGAGGGGEADD-FFQGRATSSF 60
Query: 61 PSMYDGDQCFTYSQLLASVDSLSSRLLAILRDPQLIAPTAPHRANDQLVKTCPVANELSE 120
P MY+ D+CFTYSQLLASVDSLSSRLLA +R PQL APTAP ANDQ KT PVA+ELSE
Sbjct: 61 PPMYEADRCFTYSQLLASVDSLSSRLLATVRGPQLNAPTAPRPANDQPAKTSPVASELSE 120
Query: 121 ASVELDSSNVPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKID 180
AS EL+SSN+PKIFGIYMPPSVEYII+VLSVLRCGGAFMPLDPAWPK+RILSVVS+ KID
Sbjct: 121 ASTELESSNIPKIFGIYMPPSVEYIISVLSVLRCGGAFMPLDPAWPKRRILSVVSSLKID 180
Query: 181 LIIYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHNSAVDLVFPCE----- 240
LIIYSGSSFC DGYH+T+GFRWLE+I ST F MEE+S+ EHNSAVDLVFPCE
Sbjct: 181 LIIYSGSSFCVDGYHVTEGFRWLEEISGYSTLCFNMEESSVREHNSAVDLVFPCEDEKAR 240
Query: 241 -----------------------RLLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFL 300
LLNRFQWMQE FPS+ EELLLFKTSISFIDHIQEFL
Sbjct: 241 LFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQENFPSTREELLLFKTSISFIDHIQEFL 300
Query: 301 SAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRC 360
SAILT+S L+ PPMKELKE L SVVNFIQAYSISKLTAVPSLMR LLPALQR C + +C
Sbjct: 301 SAILTASVLVTPPMKELKENLCSVVNFIQAYSISKLTAVPSLMRTLLPALQRFCGV--KC 360
Query: 361 SLRLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEIS 420
SLRLLILSGE LPI LW+ALVKLLPETT+LNLYGSTEVSGDCTYFDCK+MPMIL T+ I
Sbjct: 361 SLRLLILSGETLPILLWDALVKLLPETTILNLYGSTEVSGDCTYFDCKKMPMILETDAIK 420
Query: 421 TVPIGVPISHCDVVVVGDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGG 480
TVPIGVPISHCDVVVVGDNDALN GEL VGGPCVCSGYYSDS F PLDG SQDF+H G
Sbjct: 421 TVPIGVPISHCDVVVVGDNDALNLGELCVGGPCVCSGYYSDSVFLPLDGIKFSQDFIHEG 480
Query: 481 SLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDA 540
S N C QIYIR GDFV+QL+SGDLVFLGRKDRIIKVNGQRI+LEEIE+ALREHPDVVDA
Sbjct: 481 SFNVTCSQIYIRTGDFVQQLRSGDLVFLGRKDRIIKVNGQRISLEEIEDALREHPDVVDA 540
Query: 541 AVVSRRSDRELEYLVAFLVLKDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSS 600
AVVSR+SD ELEYLVAFLVLKDN+KS+VFRSTVRSW+VEKV LAMIPNSFFF DSIPM++
Sbjct: 541 AVVSRKSDWELEYLVAFLVLKDNEKSEVFRSTVRSWMVEKVPLAMIPNSFFFTDSIPMTT 600
Query: 601 SGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMIKEISSDDDFFTMGGN 660
SGKVDYEILTHSRPLWE HE+IDETWAN+++Q+IKKAFSDALM++EISS DDFFTMGGN
Sbjct: 601 SGKVDYEILTHSRPLWEQVHESIDETWANEFIQIIKKAFSDALMVEEISSGDDFFTMGGN 660
Query: 661 SITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGSD-IDINRDADSRKNLKIDRWNKF 720
SITAAHVS+RLG+DMRWLYHYPSPAKLLT +LEKKG D I IN DADSR+NLK DRWNK+
Sbjct: 661 SITAAHVSHRLGIDMRWLYHYPSPAKLLTVILEKKGLDIIRINEDADSRRNLKTDRWNKY 720
Query: 721 SFDDSEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISKHNKEVSDFSINLED 780
S DDSEFL HFD+ EG +SGKRKQV PN FSRA +PRNNNS +SKH K VSD SINLE+
Sbjct: 721 SLDDSEFLNHFDLKEGGSSGKRKQVQPNGDFSRAVVPRNNNSLLSKHYKAVSDCSINLEN 780
Query: 781 IGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKSPRGENGSMKKLWQV 840
I QVGGHLW S LTSVSCAFSR NKVVYE KYIG++ TL VKSPRGENGSMKKLWQV
Sbjct: 781 ISQVGGHLWHSPLTSVSCAFSRCNKVVYERKYIGDNKRAGTLLVKSPRGENGSMKKLWQV 840
Query: 841 HMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIECSTAIVGDF 900
HMESCVDASPL+VFKHP IYLFIGSHS KFVCVDAKNASL+WEIRLEGRIECS AIVGDF
Sbjct: 841 HMESCVDASPLLVFKHPNIYLFIGSHSHKFVCVDAKNASLRWEIRLEGRIECSAAIVGDF 900
Query: 901 SQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDHNLYALDYV 960
SQVVVGCYKG IYFLEFSTG I WTFQT GEVKSQPVVDP+RNLIWCGSYDHNLYALDYV
Sbjct: 901 SQVVVGCYKGNIYFLEFSTGVILWTFQTYGEVKSQPVVDPDRNLIWCGSYDHNLYALDYV 960
Query: 961 RHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSALTLWHYDLEAPVFGSLAIDPLS 1020
RHSCVYKLPCGGS+YGSPAID G +LWHYDLEAPVFGSLAIDP++
Sbjct: 961 RHSCVYKLPCGGSLYGSPAID------------GDFPFNSLWHYDLEAPVFGSLAIDPVT 1020
Query: 1021 GNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVVPSQVLICSRNGSIYSFELK 1080
NVICCLV+GHVVALDS+GSVSWK KTGGPIFAG CIS+ +PSQVLICSRNGSIYSFEL+
Sbjct: 1021 RNVICCLVDGHVVALDSSGSVSWKSKTGGPIFAGPCISTSIPSQVLICSRNGSIYSFELE 1080
Query: 1081 SGDLVWEYNIGNPITASACVDEQLQLVPETSTSSDR 1088
SGDLVWEYNIGNPITASACVDE LQLVPETS SSDR
Sbjct: 1081 SGDLVWEYNIGNPITASACVDEHLQLVPETSISSDR 1101
BLAST of Cp4.1LG12g00680 vs. NCBI nr
Match:
gi|659114660|ref|XP_008457169.1| (PREDICTED: putative acyl-activating enzyme 19 isoform X3 [Cucumis melo])
HSP 1 Score: 1736.9 bits (4497), Expect = 0.0e+00
Identity = 874/1074 (81.38%), Postives = 936/1074 (87.15%), Query Frame = 1
Query: 1 MRQLPCCISHEFQRVALSHPEKIAVIHASGGVQLCRQLHGGGGGGGGDENFFTERAISAF 60
M+Q CCISHEFQRVALSHP KIAVIHASGGVQL RQLHGGG G D FF RA S F
Sbjct: 1 MKQPLCCISHEFQRVALSHPGKIAVIHASGGVQLFRQLHGGGVGEADD--FFQGRATSDF 60
Query: 61 PSMYDGDQCFTYSQLLASVDSLSSRLLAILRDPQLIAPTAPHRANDQLVKTCPVANELSE 120
P MY+GD+CFTYSQLLASVDSLSSRLLA LR PQL APTAP ANDQ KT PVANELSE
Sbjct: 61 PPMYEGDRCFTYSQLLASVDSLSSRLLATLRRPQLNAPTAPRPANDQPAKTSPVANELSE 120
Query: 121 ASVELDSSNVPKIFGIYMPPSVEYIIAVLSVLRCGGAFMPLDPAWPKKRILSVVSTSKID 180
AS EL++ N+PKIFGIYMPPSVEYII+VLSVLRCGGAFMPLDPAWPK+RILSVVS+SKID
Sbjct: 121 ASTELETCNIPKIFGIYMPPSVEYIISVLSVLRCGGAFMPLDPAWPKRRILSVVSSSKID 180
Query: 181 LIIYSGSSFCEDGYHLTDGFRWLEQIRSCSTFSFTMEENSIPEHNSAVDLVFPCE----- 240
LIIYSGSSFCEDGYH+T+GFRWLE+I ST FTMEE+S+ EHNSAVDLVFPCE
Sbjct: 181 LIIYSGSSFCEDGYHVTEGFRWLEEISGYSTLCFTMEESSVREHNSAVDLVFPCEDEKAR 240
Query: 241 -----------------------RLLNRFQWMQELFPSSGEELLLFKTSISFIDHIQEFL 300
LLNRFQWMQE FPSS EELLLFKTSISFIDHIQEFL
Sbjct: 241 LFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQENFPSSREELLLFKTSISFIDHIQEFL 300
Query: 301 SAILTSSALIIPPMKELKEKLYSVVNFIQAYSISKLTAVPSLMRALLPALQRLCVMQNRC 360
SAILTSS L+IPPMKELKE L SVVNFIQAYSI+KLTAVPSLMR LLPALQRLC + +C
Sbjct: 301 SAILTSSVLVIPPMKELKENLCSVVNFIQAYSINKLTAVPSLMRTLLPALQRLCGV--KC 360
Query: 361 SLRLLILSGEILPIQLWNALVKLLPETTVLNLYGSTEVSGDCTYFDCKRMPMILGTEEIS 420
SLRLLILSGE LPIQLW+ALVKLLPETT+LNLYGSTEVSGDCTYFDCK+MPMIL T+ I+
Sbjct: 361 SLRLLILSGETLPIQLWDALVKLLPETTILNLYGSTEVSGDCTYFDCKKMPMILETDAIN 420
Query: 421 TVPIGVPISHCDVVVVGDNDALNQGELWVGGPCVCSGYYSDSTFHPLDGKISSQDFVHGG 480
T+PIGVPISHCDVVVVGDNDALNQGEL VGGPCVCSGYYSDS F PLDG SQDF+H G
Sbjct: 421 TIPIGVPISHCDVVVVGDNDALNQGELCVGGPCVCSGYYSDSIFLPLDGIKFSQDFIHEG 480
Query: 481 SLNANCDQIYIRMGDFVRQLQSGDLVFLGRKDRIIKVNGQRIALEEIENALREHPDVVDA 540
S N NC QIYIR GDFV+QL+SGDLVFLGRKDRIIKVNGQRI+LEEIE+ALREHPDVVDA
Sbjct: 481 SFNVNCSQIYIRTGDFVQQLRSGDLVFLGRKDRIIKVNGQRISLEEIEDALREHPDVVDA 540
Query: 541 AVVSRRSDRELEYLVAFLVLKDNKKSDVFRSTVRSWLVEKVLLAMIPNSFFFIDSIPMSS 600
AVVSR+SD ELEYLVAFLVLKDN KS+VFRS VRSW+VEKV LAMIPNSFFFIDSIP ++
Sbjct: 541 AVVSRKSDWELEYLVAFLVLKDNMKSEVFRSPVRSWMVEKVSLAMIPNSFFFIDSIPKTT 600
Query: 601 SGKVDYEILTHSRPLWEHEHETIDETWANDYMQVIKKAFSDALMIKEISSDDDFFTMGGN 660
SGKVDYEIL HSRPLWEH HE+IDETWAN+++Q+IKKAFSDALM++EISSDDDFFTMGGN
Sbjct: 601 SGKVDYEILMHSRPLWEHVHESIDETWANEFLQIIKKAFSDALMVEEISSDDDFFTMGGN 660
Query: 661 SITAAHVSYRLGVDMRWLYHYPSPAKLLTALLEKKGSD-IDINRDADSRKNLKIDRWNKF 720
SITAA VS+RLGVDMRWLYHYPSPAKLLT +LEKKG D I IN DADSR+NLK DRWNK+
Sbjct: 661 SITAALVSHRLGVDMRWLYHYPSPAKLLTVILEKKGLDIIGINGDADSRRNLKTDRWNKY 720
Query: 721 SFDDSEFLTHFDINEGQNSGKRKQVHPNDGFSRAAIPRNNNSSISKHNKEVSDFSINLED 780
S +DSEFL HFD+ EG +SGKRKQV PN GFSRA +PRNNNS +SKH K VSD SINLED
Sbjct: 721 SLNDSEFLNHFDLKEGGSSGKRKQVQPNGGFSRAVVPRNNNSLLSKHCKVVSDHSINLED 780
Query: 781 IGQVGGHLWDSLLTSVSCAFSRYNKVVYEHKYIGNSGCVETLSVKSPRGENGSMKKLWQV 840
I QVGGHLW+S LTSVSCAFSR NKVVYEHKYIG++ C TLSVKSPRGE GSMKKLWQV
Sbjct: 781 ISQVGGHLWNSPLTSVSCAFSRCNKVVYEHKYIGDNECAGTLSVKSPRGEIGSMKKLWQV 840
Query: 841 HMESCVDASPLVVFKHPKIYLFIGSHSQKFVCVDAKNASLQWEIRLEGRIECSTAIVGDF 900
HMESCVDASPL+VFKHP IYLFIGSHS KFVCVDAKNASL WEIRLEGRIECS AIVGDF
Sbjct: 841 HMESCVDASPLLVFKHPNIYLFIGSHSHKFVCVDAKNASLHWEIRLEGRIECSAAIVGDF 900
Query: 901 SQVVVGCYKGKIYFLEFSTGNIQWTFQTCGEVKSQPVVDPERNLIWCGSYDHNLYALDYV 960
SQVVVGCYKGKIYFLEFSTG IQWTFQT GEVKSQPVVDP+RNLIWCGSYDHNLYALDYV
Sbjct: 901 SQVVVGCYKGKIYFLEFSTGVIQWTFQTSGEVKSQPVVDPDRNLIWCGSYDHNLYALDYV 960
Query: 961 RHSCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRTSAL--------TLWHYDLEAPVFG 1020
RHSCVYKLPCGGS+YGSPAID VQHRLYVASTSGR SAL +LWHYDLEAPVFG
Sbjct: 961 RHSCVYKLPCGGSLYGSPAIDVVQHRLYVASTSGRISALLIKDFPFHSLWHYDLEAPVFG 1020
Query: 1021 SLAIDPLSGNVICCLVNGHVVALDSNGSVSWKCKTGGPIFAGACISSVVPSQVL 1038
SLAIDP + NVICCLV+GHVVALDS GSVSWK KTGGPIFAG CIS+ +PSQ +
Sbjct: 1021 SLAIDPFTRNVICCLVDGHVVALDSRGSVSWKSKTGGPIFAGPCISTSIPSQAV 1070
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
AEE19_ARATH | 5.1e-257 | 47.90 | Putative acyl-activating enzyme 19 OS=Arabidopsis thaliana GN=At5g35930 PE=2 SV=... | [more] |
TYCC_BREPA | 2.0e-43 | 26.76 | Tyrocidine synthase 3 OS=Brevibacillus parabrevis GN=tycC PE=1 SV=1 | [more] |
ACSF4_DANRE | 5.8e-43 | 32.27 | Acyl-CoA synthetase family member 4 OS=Danio rerio GN=aasdh PE=3 SV=1 | [more] |
LGRC_BREPA | 1.9e-41 | 26.63 | Linear gramicidin synthase subunit C OS=Brevibacillus parabrevis GN=lgrC PE=3 SV... | [more] |
ACSF4_MOUSE | 3.9e-39 | 34.70 | Acyl-CoA synthetase family member 4 OS=Mus musculus GN=Aasdh PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A061F726_THECC | 0.0e+00 | 51.62 | AMP-dependent synthetase and ligase family protein, putative isoform 1 OS=Theobr... | [more] |
B9IPY7_POPTR | 1.2e-310 | 51.55 | Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0019s06060g PE=4 SV=2 | [more] |
G7LCR6_MEDTR | 2.7e-305 | 50.04 | AMP-dependent synthetase and ligase family protein OS=Medicago truncatula GN=MTR... | [more] |
A0A0B2P8T2_GLYSO | 4.3e-303 | 50.09 | Putative acyl-activating enzyme 19 OS=Glycine soja GN=glysoja_036624 PE=4 SV=1 | [more] |
K7KCC2_SOYBN | 2.8e-302 | 49.91 | Uncharacterized protein OS=Glycine max GN=GLYMA_03G021000 PE=4 SV=1 | [more] |