Cp4.1LG11g05780 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG11g05780
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Gibberellin 3 beta-hydroxylase family protein
Location	Cp4.1LG11 : 3422254 .. 3423429 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGAGCTTCATCCCATAGGTCTTTTGACTCCTCTAGACCTCGGCACAGTTAAGGCTGTTCCTGAATCTCACGTATGGTCTCACTCCGATGAATCATCGCAGAAGATCGAATCAGCCAAATTAGTCTCGATTCCGGTTGTCGATTTTAACGACGATAATGTGTTGGAGCTCATAGGGAAAGCCTGCGAGGAGTGGGGAATGTTTCAGTTGATTAATCATGGTGTCCCGAAAACCTTAACAGCCGAAACCGAGGAGGTGGTTCGATGTCTTTTTGCTCTTCCACAAAGCCAGAAGATGAAGACATTAAATGTTCCCGGAACTGTCAGCGGCTACTGCATGGCCAGATTAACGAAGCATCATGACAAAATGATGTGGCATGAAGGATTCAACGTCATAGGTTCTCCGGTTGATGATTTTAAGAAACTCTGGCCAACAGACTACCAACGATTCTGGTAAATTCATGCAACTTTTTTCATGCATGTACACCATTAGGGTTTAGGGTTTAGGGTTTATGTTTAACTGTTTATTACTCTAAATCCCAACAATCTTATGTTTAACATTTTTTTTATTTTTTTTTTTTAATCAAGTGATATAATGGAAGAATACCAATTCAAAATGAAGGATTTAGCTGATAAGCTAATAAGCTTAATGTTCAAGTTTCTTGGCATCTCCGATGAAGAGATGGTGAAGAAATTGTCTTACATCGACCCGGCCACCGGAAAACCCCACTTGGCTTTGCGCTTGAACTCGTTTCCTCCCTGCCCTGAACCAAGCAAAGTCATTGGCATGGCAGCCCACACTGACACCTCCCTCTTCACCATGCTTCACCAAACACGCAGGGAGGGGCTACAGATCTTGAACGAGAAAGAAGGCTGGCTTCCGCTGGCTCCAAGGAGCGACGCTCTCATTATTAACATCGGCGATTTCCTCCAAATTATATCGAACGGGCGGTTTAGTAGTCTTTCTCACCGGGTGATGATACGAGAGACTGAGAAGACTACGATGTCGATGGCATATTTCTTTCATCCACCACGTCATTTATACGTAGCACCTTATTGTAAGCCATTGAGTGAAACCCTACAGACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCCTCATCAAGTCTCAAAACAACGCGAATGGAATTGCTGCCCTAAAAATATGA

mRNA sequence

ATGGCAGAGCTTCATCCCATAGGTCTTTTGACTCCTCTAGACCTCGGCACAGTTAAGGCTGTTCCTGAATCTCACGTATGGTCTCACTCCGATGAATCATCGCAGAAGATCGAATCAGCCAAATTAGTCTCGATTCCGGTTGTCGATTTTAACGACGATAATGTGTTGGAGCTCATAGGGAAAGCCTGCGAGGAGTGGGGAATGTTTCAGTTGATTAATCATGGTGTCCCGAAAACCTTAACAGCCGAAACCGAGGAGGTGGTTCGATGTCTTTTTGCTCTTCCACAAAGCCAGAAGATGAAGACATTAAATGTTCCCGGAACTGTCAGCGGCTACTGCATGGCCAGATTAACGAAGCATCATGACAAAATGATGTGGCATGAAGGATTCAACGTCATAGAATACCAATTCAAAATGAAGGATTTAGCTGATAAGCTAATAAGCTTAATGTTCAAGTTTCTTGGCATCTCCGATGAAGAGATGGTGAAGAAATTGTCTTACATCGACCCGGCCACCGGAAAACCCCACTTGGCTTTGCGCTTGAACTCGTTTCCTCCCTGCCCTGAACCAAGCAAAGTCATTGGCATGGCAGCCCACACTGACACCTCCCTCTTCACCATGCTTCACCAAACACGCAGGGAGGGGCTACAGATCTTGAACGAGAAAGAAGGCTGGCTTCCGCTGGCTCCAAGGAGCGACGCTCTCATTATTAACATCGGCGATTTCCTCCAAATTATATCGAACGGGCGGTTTAGTAGTCTTTCTCACCGGGTGATGATACGAGAGACTGAGAAGACTACGATGTCGATGGCATATTTCTTTCATCCACCACGTCATTTATACGTAGCACCTTATTGTAAGCCATTGAGTGAAACCCTACAGACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCCTCATCAAGTCTCAAAACAACGCGAATGGAATTGCTGCCCTAAAAATATGA

Coding sequence (CDS)

ATGGCAGAGCTTCATCCCATAGGTCTTTTGACTCCTCTAGACCTCGGCACAGTTAAGGCTGTTCCTGAATCTCACGTATGGTCTCACTCCGATGAATCATCGCAGAAGATCGAATCAGCCAAATTAGTCTCGATTCCGGTTGTCGATTTTAACGACGATAATGTGTTGGAGCTCATAGGGAAAGCCTGCGAGGAGTGGGGAATGTTTCAGTTGATTAATCATGGTGTCCCGAAAACCTTAACAGCCGAAACCGAGGAGGTGGTTCGATGTCTTTTTGCTCTTCCACAAAGCCAGAAGATGAAGACATTAAATGTTCCCGGAACTGTCAGCGGCTACTGCATGGCCAGATTAACGAAGCATCATGACAAAATGATGTGGCATGAAGGATTCAACGTCATAGAATACCAATTCAAAATGAAGGATTTAGCTGATAAGCTAATAAGCTTAATGTTCAAGTTTCTTGGCATCTCCGATGAAGAGATGGTGAAGAAATTGTCTTACATCGACCCGGCCACCGGAAAACCCCACTTGGCTTTGCGCTTGAACTCGTTTCCTCCCTGCCCTGAACCAAGCAAAGTCATTGGCATGGCAGCCCACACTGACACCTCCCTCTTCACCATGCTTCACCAAACACGCAGGGAGGGGCTACAGATCTTGAACGAGAAAGAAGGCTGGCTTCCGCTGGCTCCAAGGAGCGACGCTCTCATTATTAACATCGGCGATTTCCTCCAAATTATATCGAACGGGCGGTTTAGTAGTCTTTCTCACCGGGTGATGATACGAGAGACTGAGAAGACTACGATGTCGATGGCATATTTCTTTCATCCACCACGTCATTTATACGTAGCACCTTATTGTAAGCCATTGAGTGAAACCCTACAGACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCCTCATCAAGTCTCAAAACAACGCGAATGGAATTGCTGCCCTAAAAATATGA

Protein sequence

MAELHPIGLLTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKACEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDKMMWHEGFNVIEYQFKMKDLADKLISLMFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPRHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI

Homology

[Close]

BLAST of Cp4.1LG11g05780 vs. Swiss-Prot
Match: G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.6e-57
Identity = 114/337 (33.83%), Postives = 188/337 (55.79%), Query Frame = 1


Query: 14  DLGTVKAVPESHVWSHSDE----SSQKIESAKLVSIPVVDFNDDNVLELIGKACEEWGMF 73
           D  +++ +PES+ W+H D+     S  I      ++PV+D ND N  +LIG AC+ WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 74  QLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDKMMWHEG 133
           Q++NHG+P +L  + + + + LF+LP  QK K    P  VSGY +AR++    K+MW+EG
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVSGYGIARISSFFPKLMWYEG 140

Query: 134 FN-----------------------VIEYQFKMKDLADKLISLMFKFLGISDEEMVKKLS 193
           F                        V++Y   MK LA  L+ LM   LGI+ E++  K +
Sbjct: 141 FTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLMCLMLDSLGITKEDI--KWA 200

Query: 194 YIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILNEKEGWL 253
                  K   AL+LNS+P CP+P   +G+A HTD++  T+L Q    GLQ+  E  GW+
Sbjct: 201 GSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWI 260

Query: 254 PLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPRHLYVAPYC 313
            + P    L++N+GD   I+SNG + S+ HRV++  T +   S+AY + PP ++ + P+ 
Sbjct: 261 TVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRT-RQRFSVAYLYGPPSNVEICPHA 320

Query: 314 KPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
           K +  T + P+Y+ V   EY   K+++    ++++++
Sbjct: 321 KLIGPT-KPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

[Close]

BLAST of Cp4.1LG11g05780 vs. Swiss-Prot
Match: G3OX2_ARATH (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 8.5e-51
Identity = 114/345 (33.04%), Postives = 181/345 (52.46%), Query Frame = 1


Query: 5   HPIG--LLTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKA 64
           HPI   L  P D    K++P+S+ W+  D+      SA   ++P++D +D +V  L+G A
Sbjct: 12  HPIHIPLSNPPDF---KSLPDSYTWTPKDDLLFSA-SASDETLPLIDLSDIHVATLVGHA 71

Query: 65  CEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHD 124
           C  WG FQ+ NHGVP  L  + E +   LF LP  +K+K       VSGY +AR+    +
Sbjct: 72  CTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFN 131

Query: 125 KMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGISDE 184
           K MW EGF VI                       EY+  M+ LA KL+      LG+ + 
Sbjct: 132 KKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEE- 191

Query: 185 EMVKKLSYIDPATG--KPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQ 244
              K + +  P +        ++LN +P CPEP + +G+AAHTD++L T+L+Q    GLQ
Sbjct: 192 ---KDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQ 251

Query: 245 ILNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPP 304
           +  +  GW+   P   +L++N+GD L I++NG F S+ HR  +    ++  SMAY + PP
Sbjct: 252 VFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV-RSRFSMAYLWGPP 311

Query: 305 RHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALK 323
             + ++P  K L + LQ+P+Y  +  K+Y   K+ +    ++ ++
Sbjct: 312 SDIMISPLPK-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Cp4.1LG11g05780 vs. Swiss-Prot
Match: G3OX1_ARATH (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 107/335 (31.94%), Postives = 180/335 (53.73%), Query Frame = 1


Query: 14  DLGTVKAVPESHVWSHSDE---SSQKIESAKLVSIPVVDFNDDNVLELIGKACEEWGMFQ 73
           D  +++ +P+S+ W+  D+   S+     A   +IP++D +  +    IG AC  WG FQ
Sbjct: 23  DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHACRTWGAFQ 82

Query: 74  LINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDKMMWHEGF 133
           + NHGVP  L  + E +   LF LP  +K+K+      VSGY +AR+    +K MW EGF
Sbjct: 83  ISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFNKQMWSEGF 142

Query: 134 NVI-----------------------EYQFKMKDLADKLISLMFKFLGISDEEMVKKLSY 193
            +                        EY+  MK LA KL+ L    LG+S+E++  + + 
Sbjct: 143 TITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEEDI--EWAS 202

Query: 194 IDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILNEKEGWLP 253
           +         AL+LN +P CPEP + +G+AAHTD++L T+L+Q    GLQ+  +  GW+ 
Sbjct: 203 LSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVT 262

Query: 254 LAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPRHLYVAPYCK 313
           + P   +L++N+GD   I+SNG F S+ HR  + +T +  +S+A+ + P   + ++P  K
Sbjct: 263 VPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK 322

Query: 314 PLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALK 323
            L   +++P+Y+ V  KEY   K+ +    ++ ++
Sbjct: 323 -LVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Cp4.1LG11g05780 vs. Swiss-Prot
Match: G3OX4_ARATH (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 8.8e-48
Identity = 109/343 (31.78%), Postives = 182/343 (53.06%), Query Frame = 1


Query: 14  DLGTVKAVPESHVWSHSDESSQKIESAKLV------SIPVVDFNDDNVLELIGKACEEWG 73
           +LG+++ +PES  W  +   S    S+ +       SIPV+D ++ +V  LIG A + WG
Sbjct: 12  NLGSLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWG 71

Query: 74  MFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDKMMWH 133
            FQ+ NHG+ + L  + E + + LF +P  +K++  +    VSGY   R++   +K MW 
Sbjct: 72  AFQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWS 131

Query: 134 EGFNVI------------------------EYQFKMKDLADKLISLMFKFLGIS--DEEM 193
           EGF +                         EY  +M+ LA +L+  +   LG++  D E 
Sbjct: 132 EGFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEW 191

Query: 194 VKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILNE 253
             KL       G+   A+RLN +P CPEP + +G+AAHTD+++ T+LHQ+   GLQ+  E
Sbjct: 192 AHKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFRE 251

Query: 254 KEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFF-HPPRHL 313
           + GW+ + P    L++NIGD   I+SNG+  S+ HR  +  T ++ +S+AY +  P   +
Sbjct: 252 ESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHT-RSRISIAYLWGGPAGDV 311

Query: 314 YVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
            +AP  K L+   +  +Y+ +  KEY  IK +     + A+++
Sbjct: 312 QIAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of Cp4.1LG11g05780 vs. Swiss-Prot
Match: G3OX3_ARATH (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.8e-46
Identity = 109/334 (32.63%), Postives = 177/334 (52.99%), Query Frame = 1


Query: 10  LTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKACEEWGMF 69
           + PLD    K +P+SHVWS  +  +          IPV+  ++     L+ +ACEEWG+F
Sbjct: 18  IIPLDFTNTKTLPDSHVWSKPEPETTS------GPIPVISLSNPEEHGLLRQACEEWGVF 77

Query: 70  QLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDKMMWHEG 129
            + +HGV  +L    +  ++ LF+LP  +K+  +  P   +GY + R++  +DK+MW EG
Sbjct: 78  HITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWSEG 137

Query: 130 FNVI------------------------EYQFKMKDLADKLISLMFKFLGISDEEMVKKL 189
           F+V+                        EYQ  M DL+ +LIS++   LG++ E+    L
Sbjct: 138 FSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHED----L 197

Query: 190 SYIDP-----ATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILN 249
            ++ P      T      L+LNS+P CP+P   +G+A HTD+SL T+L+Q    GL+I +
Sbjct: 198 GWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIES 257

Query: 250 EKE---GWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPP 309
            +E    W+ + P   +L++ +GD   IISNG+F S  HR ++ +T    +S AYF  PP
Sbjct: 258 PQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH-RVSAAYFAGPP 317

Query: 310 RHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKS 312
           ++L + P     S+    PIY+ +  +EY   K+
Sbjct: 318 KNLQIGPL---TSDKNHPPIYRRLIWEEYLAAKA 337

[Close]

BLAST of Cp4.1LG11g05780 vs. TrEMBL
Match: O24417_CUCMA (Gibberellin 2beta,3beta-hydroxylase OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.1e-161
Identity = 284/346 (82.08%), Postives = 300/346 (86.71%), Query Frame = 1


Query: 1   MAELHPIGLLTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIG 60
           M +L  +GLLTPLDL TV+AVPESHVW H DESSQK +S K VSIPVVDFNDDNVLELIG
Sbjct: 1   MDKLPAVGLLTPLDLNTVEAVPESHVWPHLDESSQKFKSDKTVSIPVVDFNDDNVLELIG 60

Query: 61  KACEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKH 120
           KACEEWGMFQLINHG+PKTLTAETEEV RCLFALP+SQKMKTLNVPGT +GYCMARLTKH
Sbjct: 61  KACEEWGMFQLINHGIPKTLTAETEEVARCLFALPKSQKMKTLNVPGTANGYCMARLTKH 120

Query: 121 HDKMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGIS 180
           HDKMMWHEGF VI                       EYQ KMK LADKLISL+FK+LGIS
Sbjct: 121 HDKMMWHEGFTVIGSPVDDFKKLWPSDYQPFCDKVEEYQLKMKALADKLISLIFKYLGIS 180

Query: 181 DEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQ 240
           DEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKV+G+AAHTDTSLFTMLHQ RREGLQ
Sbjct: 181 DEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVMGLAAHTDTSLFTMLHQARREGLQ 240

Query: 241 ILNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPP 300
           ILNEKEGWLPLAPR DALIINIGDFLQIISNGRF S+ HRVMIRETEKTTMSMAYFFHPP
Sbjct: 241 ILNEKEGWLPLAPRRDALIINIGDFLQIISNGRFHSVPHRVMIRETEKTTMSMAYFFHPP 300

Query: 301 RHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
            HLYVAPYCKPLSETLQTPIYKGVNVKEYF+IK++ +  GIAAL I
Sbjct: 301 GHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKAKASGKGIAALTI 346

[Close]

BLAST of Cp4.1LG11g05780 vs. TrEMBL
Match: Q9LM06_CITLA (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus GN=WGA3H PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.6e-104
Identity = 182/345 (52.75%), Postives = 244/345 (70.72%), Query Frame = 1


Query: 3   ELHPIGLLTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKA 62
           EL P   L PLD  +++ VPESH+W +S E S  I+  + +S+P++DF DD   EL+G+A
Sbjct: 5   ELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASELLGRA 64

Query: 63  CEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPG-TVSGYCMARLTKHH 122
           CE+WGMFQLINHGVPKTL  E EE  R LF+LP  QKMKTL     T +GY MA ++K+H
Sbjct: 65  CEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYH 124

Query: 123 DKMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGISD 182
            K+MWHEGF +                        EYQ +MK L +++I L+FKFL IS+
Sbjct: 125 QKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISE 184

Query: 183 EEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQI 242
           EEM+K L+  D + GKP++ALRLNS+PPCP+PS+V+G+AAHTDTSLFT++HQ R +GLQ+
Sbjct: 185 EEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQV 244

Query: 243 LNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPR 302
             +  GW+PL+P S  LI+N+GD L I+SNGRF S+ HRVMI E ++   S+AYF+ PP 
Sbjct: 245 FKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPPG 304

Query: 303 HLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
            LY++PYCKPLSE+ Q P+Y+ VNVKEYF IK++    G++A+KI
Sbjct: 305 DLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of Cp4.1LG11g05780 vs. TrEMBL
Match: S0BE47_CUCSA (GA 3-oxidase OS=Cucumis sativus GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 8.7e-103
Identity = 182/346 (52.60%), Postives = 240/346 (69.36%), Query Frame = 1


Query: 3   ELHPIGLLTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKA 62
           E+ P   L PLD  +V+ VPESH+W ++ E S  I+  K VS+P++D   D   EL+G+A
Sbjct: 5   EIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASELLGRA 64

Query: 63  CEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPG--TVSGYCMARLTKH 122
           CEEWGMFQLINHG+ KTL  E EE  R LF LP +QKMKTL  PG  T +GY MA ++K+
Sbjct: 65  CEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKY 124

Query: 123 HDKMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGIS 182
           H K+MWHEGF ++                       EYQ +MK L +++I L+ KFLGIS
Sbjct: 125 HPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGIS 184

Query: 183 DEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQ 242
           +EEM+K L+  D + GKPH+ALRLNS+PPCP+P +V+G+AAHTDTSL T+LHQ    GLQ
Sbjct: 185 EEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQ 244

Query: 243 ILNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPP 302
           I  +  GW+PL+P S  LI+NIGD L I+SNGRF S+ HRVMI+E ++   S+AYF+ PP
Sbjct: 245 IFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPP 304

Query: 303 RHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
             +Y++PYCKPLS+T Q P+Y+ VNVKEYF IK++    G+ A+KI
Sbjct: 305 GEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of Cp4.1LG11g05780 vs. TrEMBL
Match: E1AXG4_9ROSI (Gibberellin 3-oxidase 1 (Fragment) OS=Marah macrocarpus PE=2 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 7.4e-94
Identity = 176/343 (51.31%), Postives = 234/343 (68.22%), Query Frame = 1


Query: 6   PIGLLTPLDLGTV-KAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVL-ELIGKAC 65
           P   L PLD  +  + VPESH+W  S E S  ++  + +S+PV+D  DD    ELIGKAC
Sbjct: 11  PSSPLVPLDFSSSHETVPESHIWVDSIELSPAMDLDEKLSLPVIDLLDDTTASELIGKAC 70

Query: 66  EEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDK 125
           ++WGMFQLINHGVPK++ AETE+  R LFALP +QKMKT   PG  +GY M  L+K+H K
Sbjct: 71  QQWGMFQLINHGVPKSIIAETEDEARRLFALPTTQKMKTFG-PGN-TGYGMVPLSKYHSK 130

Query: 126 MMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGISDEE 185
            MWHEGF +                        EYQ KMK LAD+L+ L+ KFL IS+EE
Sbjct: 131 SMWHEGFTIFGSPLDDAKKLWPSDYKRFCDVMEEYQRKMKGLADRLMRLILKFLDISEEE 190

Query: 186 MVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILN 245
           ++K L +    + K + ALRLN +PPCP+P +V+GMA+HTDTS FT++HQ R +GLQI  
Sbjct: 191 IMK-LMFTPEDSSKIYTALRLNLYPPCPDPDRVVGMASHTDTSFFTIIHQARNDGLQIFK 250

Query: 246 EKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPRHL 305
           ++ GW+PL+P S  L++N+GD LQI+SNGRF S+ HRVMI+E  +  +S+AYF+ PP H+
Sbjct: 251 DEAGWVPLSPTSGTLMVNVGDLLQILSNGRFPSILHRVMIQEKMEDRLSLAYFYTPPPHI 310

Query: 306 YVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
           Y+APYCKPLSE+ Q P+Y+ V VKEY   KS NN  G++ +KI
Sbjct: 311 YIAPYCKPLSESPQIPLYRCVTVKEYSTSKSNNNFKGLSTVKI 350

[Close]

BLAST of Cp4.1LG11g05780 vs. TrEMBL
Match: B9IKS1_POPTR (Gibberellin 3 beta-hydroxylase family protein OS=Populus trichocarpa GN=POPTR_0018s01170g PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 9.1e-68
Identity = 138/344 (40.12%), Postives = 209/344 (60.76%), Query Frame = 1


Query: 5   HPIGL--LTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKA 64
           HP+ L  + PLD  +V+ VP+SHVW     +S   ES   +SIP VD  D + ++L+G A
Sbjct: 11  HPLHLHHIIPLDFDSVRTVPDSHVWP----TSHAFESDDQLSIPTVDLMDPDAVKLVGHA 70

Query: 65  CEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHD 124
           CE WG+FQ+INHG+P  +  E E   R LF+LP   K+K L  PG  +GY +AR++    
Sbjct: 71  CETWGVFQVINHGIPLDIIDEVESEARRLFSLPTGHKLKALRSPGGATGYGLARISPFFS 130

Query: 125 KMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGISDE 184
           K MWHEGF V+                       +YQ KMK+LA  L+ L+ K L +S+E
Sbjct: 131 KKMWHEGFTVMGSPVDHARELWPNDYQRFCDVMEDYQKKMKELAITLMHLILKSLDLSEE 190

Query: 185 EMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQIL 244
           E+ K +S      G    AL+LNS+P CP+PS+V+G+A HTDTSL T+L+Q+   GL+I 
Sbjct: 191 EISKVVS-----PGGASTALQLNSYPFCPDPSRVMGLAPHTDTSLLTILYQSTINGLEIF 250

Query: 245 NEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPRH 304
            +  GW+ ++P + +L++N+GD L I+SN +F S+ HRV+++E ++  +S+AYF+ PP  
Sbjct: 251 KDGVGWVLVSPTNGSLVVNVGDLLHILSNAQFPSVLHRVVLKEKQQ-RLSLAYFYSPPTD 310

Query: 305 LYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
             V+P         Q P+Y+ V+V+EY  IK++N    ++ ++I
Sbjct: 311 FDVSPLA---LNPAQIPLYRSVSVREYIHIKAKNVEKALSLIRI 341

[Close]

BLAST of Cp4.1LG11g05780 vs. TAIR10
Match: AT1G80340.1 (AT1G80340.1 gibberellin 3-oxidase 2)

HSP 1 Score: 202.2 bits (513), Expect = 4.8e-52
Identity = 114/345 (33.04%), Postives = 181/345 (52.46%), Query Frame = 1


Query: 5   HPIG--LLTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKA 64
           HPI   L  P D    K++P+S+ W+  D+      SA   ++P++D +D +V  L+G A
Sbjct: 12  HPIHIPLSNPPDF---KSLPDSYTWTPKDDLLFSA-SASDETLPLIDLSDIHVATLVGHA 71

Query: 65  CEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHD 124
           C  WG FQ+ NHGVP  L  + E +   LF LP  +K+K       VSGY +AR+    +
Sbjct: 72  CTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFN 131

Query: 125 KMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGISDE 184
           K MW EGF VI                       EY+  M+ LA KL+      LG+ + 
Sbjct: 132 KKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEE- 191

Query: 185 EMVKKLSYIDPATG--KPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQ 244
              K + +  P +        ++LN +P CPEP + +G+AAHTD++L T+L+Q    GLQ
Sbjct: 192 ---KDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQ 251

Query: 245 ILNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPP 304
           +  +  GW+   P   +L++N+GD L I++NG F S+ HR  +    ++  SMAY + PP
Sbjct: 252 VFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV-RSRFSMAYLWGPP 311

Query: 305 RHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALK 323
             + ++P  K L + LQ+P+Y  +  K+Y   K+ +    ++ ++
Sbjct: 312 SDIMISPLPK-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Cp4.1LG11g05780 vs. TAIR10
Match: AT1G15550.1 (AT1G15550.1 gibberellin 3-oxidase 1)

HSP 1 Score: 201.8 bits (512), Expect = 6.3e-52
Identity = 107/335 (31.94%), Postives = 180/335 (53.73%), Query Frame = 1


Query: 14  DLGTVKAVPESHVWSHSDE---SSQKIESAKLVSIPVVDFNDDNVLELIGKACEEWGMFQ 73
           D  +++ +P+S+ W+  D+   S+     A   +IP++D +  +    IG AC  WG FQ
Sbjct: 23  DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHACRTWGAFQ 82

Query: 74  LINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDKMMWHEGF 133
           + NHGVP  L  + E +   LF LP  +K+K+      VSGY +AR+    +K MW EGF
Sbjct: 83  ISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFNKQMWSEGF 142

Query: 134 NVI-----------------------EYQFKMKDLADKLISLMFKFLGISDEEMVKKLSY 193
            +                        EY+  MK LA KL+ L    LG+S+E++  + + 
Sbjct: 143 TITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEEDI--EWAS 202

Query: 194 IDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILNEKEGWLP 253
           +         AL+LN +P CPEP + +G+AAHTD++L T+L+Q    GLQ+  +  GW+ 
Sbjct: 203 LSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVT 262

Query: 254 LAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPRHLYVAPYCK 313
           + P   +L++N+GD   I+SNG F S+ HR  + +T +  +S+A+ + P   + ++P  K
Sbjct: 263 VPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK 322

Query: 314 PLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALK 323
            L   +++P+Y+ V  KEY   K+ +    ++ ++
Sbjct: 323 -LVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Cp4.1LG11g05780 vs. TAIR10
Match: AT1G80330.1 (AT1G80330.1 gibberellin 3-oxidase 4)

HSP 1 Score: 192.2 bits (487), Expect = 5.0e-49
Identity = 109/343 (31.78%), Postives = 182/343 (53.06%), Query Frame = 1


Query: 14  DLGTVKAVPESHVWSHSDESSQKIESAKLV------SIPVVDFNDDNVLELIGKACEEWG 73
           +LG+++ +PES  W  +   S    S+ +       SIPV+D ++ +V  LIG A + WG
Sbjct: 12  NLGSLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWG 71

Query: 74  MFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDKMMWH 133
            FQ+ NHG+ + L  + E + + LF +P  +K++  +    VSGY   R++   +K MW 
Sbjct: 72  AFQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWS 131

Query: 134 EGFNVI------------------------EYQFKMKDLADKLISLMFKFLGIS--DEEM 193
           EGF +                         EY  +M+ LA +L+  +   LG++  D E 
Sbjct: 132 EGFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEW 191

Query: 194 VKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILNE 253
             KL       G+   A+RLN +P CPEP + +G+AAHTD+++ T+LHQ+   GLQ+  E
Sbjct: 192 AHKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFRE 251

Query: 254 KEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFF-HPPRHL 313
           + GW+ + P    L++NIGD   I+SNG+  S+ HR  +  T ++ +S+AY +  P   +
Sbjct: 252 ESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHT-RSRISIAYLWGGPAGDV 311

Query: 314 YVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
            +AP  K L+   +  +Y+ +  KEY  IK +     + A+++
Sbjct: 312 QIAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of Cp4.1LG11g05780 vs. TAIR10
Match: AT4G21690.1 (AT4G21690.1 gibberellin 3-oxidase 3)

HSP 1 Score: 187.2 bits (474), Expect = 1.6e-47
Identity = 109/334 (32.63%), Postives = 177/334 (52.99%), Query Frame = 1


Query: 10  LTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKACEEWGMF 69
           + PLD    K +P+SHVWS  +  +          IPV+  ++     L+ +ACEEWG+F
Sbjct: 18  IIPLDFTNTKTLPDSHVWSKPEPETTS------GPIPVISLSNPEEHGLLRQACEEWGVF 77

Query: 70  QLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDKMMWHEG 129
            + +HGV  +L    +  ++ LF+LP  +K+  +  P   +GY + R++  +DK+MW EG
Sbjct: 78  HITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWSEG 137

Query: 130 FNVI------------------------EYQFKMKDLADKLISLMFKFLGISDEEMVKKL 189
           F+V+                        EYQ  M DL+ +LIS++   LG++ E+    L
Sbjct: 138 FSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHED----L 197

Query: 190 SYIDP-----ATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILN 249
            ++ P      T      L+LNS+P CP+P   +G+A HTD+SL T+L+Q    GL+I +
Sbjct: 198 GWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIES 257

Query: 250 EKE---GWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPP 309
            +E    W+ + P   +L++ +GD   IISNG+F S  HR ++ +T    +S AYF  PP
Sbjct: 258 PQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH-RVSAAYFAGPP 317

Query: 310 RHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKS 312
           ++L + P     S+    PIY+ +  +EY   K+
Sbjct: 318 KNLQIGPL---TSDKNHPPIYRRLIWEEYLAAKA 337

[Close]

BLAST of Cp4.1LG11g05780 vs. TAIR10
Match: AT1G17020.1 (AT1G17020.1 senescence-related gene 1)

HSP 1 Score: 154.5 bits (389), Expect = 1.1e-37
Identity = 98/323 (30.34%), Postives = 164/323 (50.77%), Query Frame = 1


Query: 17  TVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFND-------DNVLELIGKACEEWGMF 76
           T+  VP  +V S  D++    +    + IP++D          D+ +E +  AC+EWG F
Sbjct: 25  TITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFF 84

Query: 77  QLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDKMMWHEG 136
           QL+NHG+  +   + +  ++  F LP  +K K    P  + G+  A +     K+ W + 
Sbjct: 85  QLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADL 144

Query: 137 F-----------------------NVIE-YQFKMKDLADKLISLMFKFLGISDEEMVKKL 196
           F                       + +E Y  +++ +A  LI+ M + L I  EE+ K  
Sbjct: 145 FFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLF 204

Query: 197 SYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRR-EGLQILNEKEG 256
             +D        ++R+N +PPCP+P +VIG+  H+D+   T+L Q    EGLQI  +K+G
Sbjct: 205 DDVDSVQ-----SMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQI--KKDG 264

Query: 257 -WLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPRHLYVA 307
            W+P+ P  +A I+NIGD L+II+NG + S+ HR ++  +EK  +S+A F +   +  V 
Sbjct: 265 KWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVV-NSEKERLSIATFHNVGMYKEVG 324

[Close]

BLAST of Cp4.1LG11g05780 vs. NCBI nr
Match: gi|2289032|gb|AAB64347.1| (gibberellin 2beta,3beta-hydroxylase [Cucurbita maxima])

HSP 1 Score: 577.4 bits (1487), Expect = 1.6e-161
Identity = 284/346 (82.08%), Postives = 300/346 (86.71%), Query Frame = 1


Query: 1   MAELHPIGLLTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIG 60
           M +L  +GLLTPLDL TV+AVPESHVW H DESSQK +S K VSIPVVDFNDDNVLELIG
Sbjct: 1   MDKLPAVGLLTPLDLNTVEAVPESHVWPHLDESSQKFKSDKTVSIPVVDFNDDNVLELIG 60

Query: 61  KACEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKH 120
           KACEEWGMFQLINHG+PKTLTAETEEV RCLFALP+SQKMKTLNVPGT +GYCMARLTKH
Sbjct: 61  KACEEWGMFQLINHGIPKTLTAETEEVARCLFALPKSQKMKTLNVPGTANGYCMARLTKH 120

Query: 121 HDKMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGIS 180
           HDKMMWHEGF VI                       EYQ KMK LADKLISL+FK+LGIS
Sbjct: 121 HDKMMWHEGFTVIGSPVDDFKKLWPSDYQPFCDKVEEYQLKMKALADKLISLIFKYLGIS 180

Query: 181 DEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQ 240
           DEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKV+G+AAHTDTSLFTMLHQ RREGLQ
Sbjct: 181 DEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVMGLAAHTDTSLFTMLHQARREGLQ 240

Query: 241 ILNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPP 300
           ILNEKEGWLPLAPR DALIINIGDFLQIISNGRF S+ HRVMIRETEKTTMSMAYFFHPP
Sbjct: 241 ILNEKEGWLPLAPRRDALIINIGDFLQIISNGRFHSVPHRVMIRETEKTTMSMAYFFHPP 300

Query: 301 RHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
            HLYVAPYCKPLSETLQTPIYKGVNVKEYF+IK++ +  GIAAL I
Sbjct: 301 GHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKAKASGKGIAALTI 346

[Close]

BLAST of Cp4.1LG11g05780 vs. NCBI nr
Match: gi|8894936|gb|AAF80661.1|AF074710_1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 386.3 bits (991), Expect = 5.1e-104
Identity = 182/345 (52.75%), Postives = 244/345 (70.72%), Query Frame = 1


Query: 3   ELHPIGLLTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKA 62
           EL P   L PLD  +++ VPESH+W +S E S  I+  + +S+P++DF DD   EL+G+A
Sbjct: 5   ELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASELLGRA 64

Query: 63  CEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPG-TVSGYCMARLTKHH 122
           CE+WGMFQLINHGVPKTL  E EE  R LF+LP  QKMKTL     T +GY MA ++K+H
Sbjct: 65  CEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYH 124

Query: 123 DKMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGISD 182
            K+MWHEGF +                        EYQ +MK L +++I L+FKFL IS+
Sbjct: 125 QKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISE 184

Query: 183 EEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQI 242
           EEM+K L+  D + GKP++ALRLNS+PPCP+PS+V+G+AAHTDTSLFT++HQ R +GLQ+
Sbjct: 185 EEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQV 244

Query: 243 LNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPR 302
             +  GW+PL+P S  LI+N+GD L I+SNGRF S+ HRVMI E ++   S+AYF+ PP 
Sbjct: 245 FKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPPG 304

Query: 303 HLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
            LY++PYCKPLSE+ Q P+Y+ VNVKEYF IK++    G++A+KI
Sbjct: 305 DLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of Cp4.1LG11g05780 vs. NCBI nr
Match: gi|568384792|ref|NP_001275523.1| (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus])

HSP 1 Score: 381.7 bits (979), Expect = 1.3e-102
Identity = 182/346 (52.60%), Postives = 240/346 (69.36%), Query Frame = 1


Query: 3   ELHPIGLLTPLDLGTVKAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGKA 62
           E+ P   L PLD  +V+ VPESH+W ++ E S  I+  K VS+P++D   D   EL+G+A
Sbjct: 5   EIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASELLGRA 64

Query: 63  CEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPG--TVSGYCMARLTKH 122
           CEEWGMFQLINHG+ KTL  E EE  R LF LP +QKMKTL  PG  T +GY MA ++K+
Sbjct: 65  CEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKY 124

Query: 123 HDKMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGIS 182
           H K+MWHEGF ++                       EYQ +MK L +++I L+ KFLGIS
Sbjct: 125 HPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKFLGIS 184

Query: 183 DEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQ 242
           +EEM+K L+  D + GKPH+ALRLNS+PPCP+P +V+G+AAHTDTSL T+LHQ    GLQ
Sbjct: 185 EEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQ 244

Query: 243 ILNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPP 302
           I  +  GW+PL+P S  LI+NIGD L I+SNGRF S+ HRVMI+E ++   S+AYF+ PP
Sbjct: 245 IFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPP 304

Query: 303 RHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
             +Y++PYCKPLS+T Q P+Y+ VNVKEYF IK++    G+ A+KI
Sbjct: 305 GEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of Cp4.1LG11g05780 vs. NCBI nr
Match: gi|659088742|ref|XP_008445138.1| (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 374.0 bits (959), Expect = 2.6e-100
Identity = 179/347 (51.59%), Postives = 244/347 (70.32%), Query Frame = 1


Query: 3   ELHPIGLLTPLDLGTVKA-VPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVLELIGK 62
           E+ P   L PL+  +V+  VPESH+W +  E S  ++  K VS+P++D  +D+  EL+G+
Sbjct: 5   EIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASELLGR 64

Query: 63  ACEEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPG--TVSGYCMARLTK 122
           ACEEWGMFQLINHGV +TL  E EE  R LF LP +QKMKTL  PG  T +GY MA ++K
Sbjct: 65  ACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMAGISK 124

Query: 123 HHDKMMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGI 182
           +H K+MWHEGF +I                       EYQ +MK L +++I L+ KFLGI
Sbjct: 125 YHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILKFLGI 184

Query: 183 SDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGL 242
           S+EEM+K L+  D + GKP++ALRLNS+PPCP+P +V+G+AAHTDTSL T+LHQ R  GL
Sbjct: 185 SEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVRSNGL 244

Query: 243 QILNEKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHP 302
           QI  +  GW+PL+P S  L++N+GD L I+SNGRF+S+ HRVMI+E ++   S+AYF+ P
Sbjct: 245 QIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAYFYDP 304

Query: 303 PRHLYVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
           P  +Y++PYCKPLS+T Q P+Y+ VNVKEYF IK++   +G+ A+KI
Sbjct: 305 PGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

[Close]

BLAST of Cp4.1LG11g05780 vs. NCBI nr
Match: gi|304636271|gb|ADM47337.1| (gibberellin 3-oxidase 1 [Marah macrocarpus])

HSP 1 Score: 352.1 bits (902), Expect = 1.1e-93
Identity = 176/343 (51.31%), Postives = 234/343 (68.22%), Query Frame = 1


Query: 6   PIGLLTPLDLGTV-KAVPESHVWSHSDESSQKIESAKLVSIPVVDFNDDNVL-ELIGKAC 65
           P   L PLD  +  + VPESH+W  S E S  ++  + +S+PV+D  DD    ELIGKAC
Sbjct: 11  PSSPLVPLDFSSSHETVPESHIWVDSIELSPAMDLDEKLSLPVIDLLDDTTASELIGKAC 70

Query: 66  EEWGMFQLINHGVPKTLTAETEEVVRCLFALPQSQKMKTLNVPGTVSGYCMARLTKHHDK 125
           ++WGMFQLINHGVPK++ AETE+  R LFALP +QKMKT   PG  +GY M  L+K+H K
Sbjct: 71  QQWGMFQLINHGVPKSIIAETEDEARRLFALPTTQKMKTFG-PGN-TGYGMVPLSKYHSK 130

Query: 126 MMWHEGFNVI-----------------------EYQFKMKDLADKLISLMFKFLGISDEE 185
            MWHEGF +                        EYQ KMK LAD+L+ L+ KFL IS+EE
Sbjct: 131 SMWHEGFTIFGSPLDDAKKLWPSDYKRFCDVMEEYQRKMKGLADRLMRLILKFLDISEEE 190

Query: 186 MVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGMAAHTDTSLFTMLHQTRREGLQILN 245
           ++K L +    + K + ALRLN +PPCP+P +V+GMA+HTDTS FT++HQ R +GLQI  
Sbjct: 191 IMK-LMFTPEDSSKIYTALRLNLYPPCPDPDRVVGMASHTDTSFFTIIHQARNDGLQIFK 250

Query: 246 EKEGWLPLAPRSDALIINIGDFLQIISNGRFSSLSHRVMIRETEKTTMSMAYFFHPPRHL 305
           ++ GW+PL+P S  L++N+GD LQI+SNGRF S+ HRVMI+E  +  +S+AYF+ PP H+
Sbjct: 251 DEAGWVPLSPTSGTLMVNVGDLLQILSNGRFPSILHRVMIQEKMEDRLSLAYFYTPPPHI 310

Query: 306 YVAPYCKPLSETLQTPIYKGVNVKEYFLIKSQNNANGIAALKI 324
           Y+APYCKPLSE+ Q P+Y+ V VKEY   KS NN  G++ +KI
Sbjct: 311 YIAPYCKPLSESPQIPLYRCVTVKEYSTSKSNNNFKGLSTVKI 350

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g05780 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G3OX_PEA	3.6e-57	33.83	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1	[more]
G3OX2_ARATH	8.5e-51	33.04	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA3OX2 PE=1 SV=2	[more]
G3OX1_ARATH	1.1e-50	31.94	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2	[more]
G3OX4_ARATH	8.8e-48	31.78	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1	[more]
G3OX3_ARATH	2.8e-46	32.63	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA3OX3 PE=2 SV=1	[more]

BLAST of Cp4.1LG11g05780 vs. TrEMBL


Match Name	E-value	Identity	Description
O24417_CUCMA	1.1e-161	82.08	Gibberellin 2beta,3beta-hydroxylase OS=Cucurbita maxima PE=2 SV=1	[more]
Q9LM06_CITLA	3.6e-104	52.75	Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus GN=WGA3H PE=2 SV=1	[more]
S0BE47_CUCSA	8.7e-103	52.60	GA 3-oxidase OS=Cucumis sativus GN=GA3ox4 PE=2 SV=1	[more]
E1AXG4_9ROSI	7.4e-94	51.31	Gibberellin 3-oxidase 1 (Fragment) OS=Marah macrocarpus PE=2 SV=1	[more]
B9IKS1_POPTR	9.1e-68	40.12	Gibberellin 3 beta-hydroxylase family protein OS=Populus trichocarpa GN=POPTR_00...	[more]

BLAST of Cp4.1LG11g05780 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G80340.1	4.8e-52	33.04	gibberellin 3-oxidase 2	[more]
AT1G15550.1	6.3e-52	31.94	gibberellin 3-oxidase 1	[more]
AT1G80330.1	5.0e-49	31.78	gibberellin 3-oxidase 4	[more]
AT4G21690.1	1.6e-47	32.63	gibberellin 3-oxidase 3	[more]
AT1G17020.1	1.1e-37	30.34	senescence-related gene 1	[more]

BLAST of Cp4.1LG11g05780 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|2289032\|gb\|AAB64347.1\|	1.6e-161	82.08	gibberellin 2beta,3beta-hydroxylase [Cucurbita maxima]	[more]
gi\|8894936\|gb\|AAF80661.1\|AF074710_1	5.1e-104	52.75	putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris]	[more]
gi\|568384792\|ref\|NP_001275523.1\|	1.3e-102	52.60	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus]	[more]
gi\|659088742\|ref\|XP_008445138.1\|	2.6e-100	51.59	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]
gi\|304636271\|gb\|ADM47337.1\|	1.1e-93	51.31	gibberellin 3-oxidase 1 [Marah macrocarpus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process
GO:0006508	proteolysis

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0008237	metallopeptidase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR026992	DIOX_N
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR001261	ArgE/DapE_CS

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g05780.1	Cp4.1LG11g05780.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001261	ArgE/DapE/ACY1/CPG2/YscS, conserved site	PROSITE	PS00758	ARGE_DAPE_CPG2_1	coord: 194..203 scor
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 177..276 score: 5.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 174..276 score: 9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 45..135 score: 7.1
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 18..316 score: 1.1
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..320 score: 1.8E
None	No IPR available	PANTHER	PTHR10209:SF197	GIBBERELLIN 3-BETA-DIOXYGENASE 3	coord: 1..320 score: 1.8E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 13..313 score: 2.13

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG11g05780	Cp4.1LG07g01930	Cucurbita pepo (Zucchini)	cpecpeB138

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG11g05780	Silver-seed gourd	carcpeB0079
Cp4.1LG11g05780	Silver-seed gourd	carcpeB1351
Cp4.1LG11g05780	Silver-seed gourd	carcpeB1353
Cp4.1LG11g05780	Cucumber (Chinese Long) v3	cpecucB0107
Cp4.1LG11g05780	Cucumber (Chinese Long) v3	cpecucB0125
Cp4.1LG11g05780	Wax gourd	cpewgoB0144
Cp4.1LG11g05780	Cucurbita pepo (Zucchini)	cpecpeB151
Cp4.1LG11g05780	Cucumber (Gy14) v1	cgycpeB0428
Cp4.1LG11g05780	Cucurbita maxima (Rimu)	cmacpeB174
Cp4.1LG11g05780	Cucurbita maxima (Rimu)	cmacpeB464
Cp4.1LG11g05780	Cucurbita maxima (Rimu)	cmacpeB766
Cp4.1LG11g05780	Cucurbita moschata (Rifu)	cmocpeB148
Cp4.1LG11g05780	Cucurbita moschata (Rifu)	cmocpeB423
Cp4.1LG11g05780	Cucurbita moschata (Rifu)	cmocpeB720
Cp4.1LG11g05780	Wild cucumber (PI 183967)	cpecpiB093
Cp4.1LG11g05780	Cucumber (Chinese Long) v2	cpecuB097
Cp4.1LG11g05780	Bottle gourd (USVL1VR-Ls)	cpelsiB098
Cp4.1LG11g05780	Watermelon (Charleston Gray)	cpewcgB122
Cp4.1LG11g05780	Watermelon (97103) v1	cpewmB099
Cp4.1LG11g05780	Melon (DHL92) v3.5.1	cpemeB092
Cp4.1LG11g05780	Cucumber (Gy14) v2	cgybcpeB162
Cp4.1LG11g05780	Melon (DHL92) v3.6.1	cpemedB116