Cp4.1LG10g04470 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG10g04470
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Glutamate-ammonia ligase-like protein
Location	Cp4.1LG10 : 1455683 .. 1459899 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGGGAATCGTGTTCCCGCCAAATTTAAATTGGAGAGGAAATGCGAACGGTCCGTCATTCGCGCTACTTCAGAGCTTGGTGGGCGAAGGGAGAAGTGAAAGAGACCGCCGGAGAAAGGAAAGAAGATGGATTTCAGTGTTCTGAAGAAAGAGGTGGACGAAGCCGTGCTGGTTGACGCTCACGCCCCCAACGACGCCAAGCTTGTCCGGATTATTTGGGTCGATGGTTCGGGACAACATCGATGTCGTGTAAGATTTATTTATTATTACACACTTTTCAAATTTGAATTTAATTTTTATTTAGTATCCAGTTTTAAAATGTTAGATTATGTTATAATTATTCAGGCTGTTCCATTTAAGCGGTTCAATGATGTCACTAAAAGGAATGGGATTGGGCTACCTTTTGCCGTTATGGCAGCCAGTTCTTGTAATGATTGCCCAGCTGCTGGGAGTAATCTTAGCAGTGTGGGCGAGATGAGTCTGATGCCAGATTTATCAACCAAACGGACGGTTCCTTGGTATGGAGCTTTCAATTCTTCTATCATGTAAAAATTATCTCTGTTCGTTCAAGTAGGGCATTGATTAGTCATACCATGAGAGGAAGGACTCGATATTGACATGTCTTACAATTTACTTGTTTGTACGTGTTGGGTATGCCAAGATTTGTATTTTTACCGACTCAATGTCACTGCTAACCAGCTAATTAGTTTAGTTCATTAAACCCTTGAGAAGTTCAGTTCGTTGTGATCAAATTATGCAGACATTTGCATTTTTACAGACCCCGTAACTAAACTCTAGATGAATATGCATATTTTATGTCAATTGAGTTTGGAGTGGAAGCATTTGGTAGTGTAATTGGGATTTTGTATAGCTAACTATTTGTCCATTAGAGTCCAATTTTTAAGTCCTTTGTGCCCACTTTCGGTTAGGAATCACGACCCTCCACAATGAGATTTTGTATAGCTAACTATTTGTCTTCTGGAGTCTAATTTTTAAGTCCTTTGGGCCCACGTTCGGTTAGGAACCACGACCCTCCATAATGGTATGCTATTGTCAAAGTTTTCGTGGATTTACTTTTAGTTTCTTTAAAAGGCCTCGTATCAATGGAGATACTATTTCTTACTTATAAACCGATAATCTTCCCTTTAGTTAGTCAACATGAACTACTCCTCCTAATAATCGAAAAGTAGATCGAAACATACCTATTCCATTGATTTCTTGAACAGGAACAAGCAGGAGGAGATGGTTATGGGTGATATGCAGCTTAGACCTGGTGAAGCCTGGGAGTACTGTCCAAGGGAAGCCTTACGAAGGGTGTCTAGAATCCTGAAAGATGAATTTAACCTGGTAGGGAAGATTGTTCAAGTCTTATGATGAATAGATGATGTAGAAAATATTTTTACTGTGTTAAATGGCTGTCTTGAATCCAGGTAATGAAAGCAGGGTTTGAAATTGAGTTCTTACTGTTAAAAAAGGCAGTTAGGTAAGGATATACTATTCACTTCTCTTCTCTATGGCCTTAATTCATTTCAAATTGTTAAATTTCTATTGCTCTATTTAATTTGAGTTTCGTAGAGATGGGAAGGAAGATTGGGTGCCGTTTGATTCAACGCCCTATTGTTCTGCACTTTCATATGATGCTGCCTCTCCTTTTCTTCATGAAGTTGTAGCTTCTTTGAACTCCTTGAATATTACCGTGGAACAGGTATGCCAAAAAGGTCCTTAAGATACGTTTTGAAACTGTGAGACTGACGGTGATACGTAACGGACTAAGACAGATAATATCTGTCAGCAGTAGATCATATAAGATGAAGCGGTAGAGTTTCAAGCTAATGCATATTGATAGGGATTTTTGTTGTGTCTTGTAGTTGCATGCAGAGGCTGGGAAAGGTCAATTTGAGATTGTTTTAGGGTATACTGCTTGTCTCAATGCTGCTGACAACTTAATTTACACGCAAGAAGTGATCAAGGCTACTGCAAGGAAGCATGGATTGTTGGCAACATTTTTGCCCAAGTATGCAATGTTCTAAAATATGATGCGACCTGAAGTTCTAAAATAAATAACATCATGGTTTCTTATCATTGCTTAGGTATGCAATGGATGACGTTGGTTCTGGCTCCCACGTGCATGTCAGCTTGTGGCAGAATGATAAAAATGTTTTCATGGCGGCTGAAGGCTCTTCTCAACATGGAATGTCAGCCGTCGGGGAAGAGTTCATGGCAGGCGTTTTACATCATATTCCAGCAATTTTGGCATTTATCTGTCCAATCCCCAACAGGTGAACACCCCCGCTTCATATTCTCTATGTATTCTTTATAAATGTGATGTCCGGGTTGGTTGAGGAGGAGAACAAACCACCATTTATAAGGGGTGGAAACCTTCTCCTATTAAACCTGTTTTAAAGCCTTGAGGGCAAGCCCGAAAGGGAAAGCCTAAAGAGGACAATATCTGCTAGCGGTGGATTTAGGTCGTTACAAATGGTATCAGAGCTAGACACGGGACGATGTGCCAGCCTTCTCGCTGTTCCCCAAAGGGGGGTAGACACGAGGTGGTGTGCCAGTAAGGATGCTGGGCCCCAAAGGGAGTGGATTTGGGGGCGGTCCCACATCGATTGGAGGAAGGAAAGAGTGCCAGCGAGGACGCTGGGCCTCGAAGGGGGTGGATTGTGATGTCCCACATTGGTTGGAGAGGAGAACAAACCACCATTTATAAGGGTGTGGAAACCTTCCCCTAGTAGACGCGTTTTAAAGCCTGGATTGTGATGTCCCACATTGGTTGGAGAGGAGAACAAACCACCATTTATAAGGGTGTGGAAACCTTCCCCTAGTAGACGCGTTTTAAAGCCTGGATTGTGATGTCCCACATTGGTTGGAGAGGAGAACAAACCACCATTTATAAGGGTGTGGAAACCTTCCCCTAGTAGACGCGTTTTAAAGCCTGGATTGTGATGTCCCACATTGGTTGGAGAGGAGAACAAACCACCATTTATAAGGGTGTGGAAACCTTCACCTAGTAGACGCGTTTTAAAGCCTGGATTGTGATGTCCCACATTGGTTGGAGAGGAGAACAATCCACCATTTATAAGGGTGTGAAAACCTTTCCCTAGTAGACGCGTTTTAAAGCGAGGGCAAGCCCGAAAGGGAAAGCCCAAAGAGAACAATATCAGCTAAGCTATCAGTGGGTTTGGGCTGTTACAAATGGTATCAAAGCCAGACACTAAATAGTGTGAGTGTGCCACCGAGATGCTGGCTCCAAGGGAGTGGATTGTGAGATCTCACATCAGTTGGAGAGGGAAACGAATCATTCCGCTGGCTCCAAGGGAGTGGATTGTGAGATCTCACATCAGAAGGGAGTGGATTGACGCGTTTTAAAACCTTGAGATGAAGTCTTTAAAGGGAAAGTCGAAAGAGGACAATATCTACTGCCGGTGAAGTGGCTTGACTTCTAACACGTCCCTGCCAACTTGTTTGCTTGTAGTTATGCTCGTTTACAACCCAATACATGGAGTGGAGCCTATCAATGTTGGGGAAAGGATAACAGAGAAGCTCCAATTAGAACTGCTTGTCCTCCTGGAATTCGTGAAGGTTCAGTCAGTAACTTTGAGATCAAATCATTTGACGGTTGCGCAAACCCATACTTGGGTCTAGCCGCTATCCTTTCTGGTGGACTAGATGGCCTTCGGCACCATCTTCAGTTGCCTGAACCCATTGGTAAATTTCCTGTTTCCAATAGGTTATTATGTTATTGGTAAAGTTTCATGTTGTACCTCATAACTTGAATCATATAATAAACAGCCTTCAATCCTTATGAGAATGCTTCAAAGCTCAAAAGGTTGCCCCAATCGCTTGCTGAATCTGTAGAGGCTCTGGAAAAGAACAATACCTTGACAGAGCTTATAGGTGAAAAGTTGGTGGTTGCCATAAAGGCAGTTCGCAAGGTATGTATGCCATTTGGATGTGTTTTGTTTCTTTTGATCAGTTCTACATGGGCTCTTCATTTTGATTGATAATCTGTAAAACATTGTGTCATTTGCAGGCAGAGGTGGAATACCACTCAAAGCATCAGGAGGCATACAAGCAACTTATACACAACTATTAAGGACTGACTCAAACACCTTAAAGATTTATAATTATGATATTTTATGAACACTTTATAAAGGTTTTATAATAAGAATCTCATTATAATGTTTTAACTTCGACTTAAAAGTGAAGTTTTTG

mRNA sequence

ACGGGAATCGTGTTCCCGCCAAATTTAAATTGGAGAGGAAATGCGAACGGTCCGTCATTCGCGCTACTTCAGAGCTTGGTGGGCGAAGGGAGAAGTGAAAGAGACCGCCGGAGAAAGGAAAGAAGATGGATTTCAGTGTTCTGAAGAAAGAGGTGGACGAAGCCGTGCTGGTTGACGCTCACGCCCCCAACGACGCCAAGCTTGTCCGGATTATTTGGGTCGATGGTTCGGGACAACATCGATGTCGTGCTGTTCCATTTAAGCGGTTCAATGATGTCACTAAAAGGAATGGGATTGGGCTACCTTTTGCCGTTATGGCAGCCAGTTCTTGTAATGATTGCCCAGCTGCTGGGAGTAATCTTAGCAGTGTGGGCGAGATGAGTCTGATGCCAGATTTATCAACCAAACGGACGGTTCCTTGGAACAAGCAGGAGGAGATGGTTATGGGTGATATGCAGCTTAGACCTGGTGAAGCCTGGGAGTACTGTCCAAGGGAAGCCTTACGAAGGGTGTCTAGAATCCTGAAAGATGAATTTAACCTGGTAATGAAAGCAGGGTTTGAAATTGAGTTCTTACTGTTAAAAAAGGCAGTTAGAGATGGGAAGGAAGATTGGGTGCCGTTTGATTCAACGCCCTATTGTTCTGCACTTTCATATGATGCTGCCTCTCCTTTTCTTCATGAATTGCATGCAGAGGCTGGGAAAGGTCAATTTGAGATTGTTTTAGGGTATACTGCTTGTCTCAATGCTGCTGACAACTTAATTTACACGCAAGAAGTGATCAAGGCTACTGCAAGGAAGCATGGATTGTTGGCAACATTTTTGCCCAAGTATGCAATGGATGACGTTGGTTCTGGCTCCCACGTGCATGTCAGCTTGTGGCAGAATGATAAAAATGTTTTCATGGCGGCTGAAGGCTCTTCTCAACATGGAATAGAAGCTCCAATTAGAACTGCTTGTCCTCCTGGAATTCGTGAAGGTTCAGTCAGTAACTTTGAGATCAAATCATTTGACGGTTGCGCAAACCCATACTTGGGTCTAGCCGCTATCCTTTCTGGTGGACTAGATGGCCTTCGGCACCATCTTCAGTTGCCTGAACCCATTGCCTTCAATCCTTATGAGAATGCTTCAAAGCTCAAAAGGTTGCCCCAATCGCTTGCTGAATCTGTAGAGGCTCTGGAAAAGAACAATACCTTGACAGAGCTTATAGGTGAAAAGTTGGTGGTTGCCATAAAGGCAGTTCGCAAGGCAGAGGTGGAATACCACTCAAAGCATCAGGAGGCATACAAGCAACTTATACACAACTATTAAGGACTGACTCAAACACCTTAAAGATTTATAATTATGATATTTTATGAACACTTTATAAAGGTTTTATAATAAGAATCTCATTATAATGTTTTAACTTCGACTTAAAAGTGAAGTTTTTG

Coding sequence (CDS)

ATGGATTTCAGTGTTCTGAAGAAAGAGGTGGACGAAGCCGTGCTGGTTGACGCTCACGCCCCCAACGACGCCAAGCTTGTCCGGATTATTTGGGTCGATGGTTCGGGACAACATCGATGTCGTGCTGTTCCATTTAAGCGGTTCAATGATGTCACTAAAAGGAATGGGATTGGGCTACCTTTTGCCGTTATGGCAGCCAGTTCTTGTAATGATTGCCCAGCTGCTGGGAGTAATCTTAGCAGTGTGGGCGAGATGAGTCTGATGCCAGATTTATCAACCAAACGGACGGTTCCTTGGAACAAGCAGGAGGAGATGGTTATGGGTGATATGCAGCTTAGACCTGGTGAAGCCTGGGAGTACTGTCCAAGGGAAGCCTTACGAAGGGTGTCTAGAATCCTGAAAGATGAATTTAACCTGGTAATGAAAGCAGGGTTTGAAATTGAGTTCTTACTGTTAAAAAAGGCAGTTAGAGATGGGAAGGAAGATTGGGTGCCGTTTGATTCAACGCCCTATTGTTCTGCACTTTCATATGATGCTGCCTCTCCTTTTCTTCATGAATTGCATGCAGAGGCTGGGAAAGGTCAATTTGAGATTGTTTTAGGGTATACTGCTTGTCTCAATGCTGCTGACAACTTAATTTACACGCAAGAAGTGATCAAGGCTACTGCAAGGAAGCATGGATTGTTGGCAACATTTTTGCCCAAGTATGCAATGGATGACGTTGGTTCTGGCTCCCACGTGCATGTCAGCTTGTGGCAGAATGATAAAAATGTTTTCATGGCGGCTGAAGGCTCTTCTCAACATGGAATAGAAGCTCCAATTAGAACTGCTTGTCCTCCTGGAATTCGTGAAGGTTCAGTCAGTAACTTTGAGATCAAATCATTTGACGGTTGCGCAAACCCATACTTGGGTCTAGCCGCTATCCTTTCTGGTGGACTAGATGGCCTTCGGCACCATCTTCAGTTGCCTGAACCCATTGCCTTCAATCCTTATGAGAATGCTTCAAAGCTCAAAAGGTTGCCCCAATCGCTTGCTGAATCTGTAGAGGCTCTGGAAAAGAACAATACCTTGACAGAGCTTATAGGTGAAAAGTTGGTGGTTGCCATAAAGGCAGTTCGCAAGGCAGAGGTGGAATACCACTCAAAGCATCAGGAGGCATACAAGCAACTTATACACAACTATTAA

Protein sequence

MDFSVLKKEVDEAVLVDAHAPNDAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSSVGEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVMKAGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHELHAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHVHVSLWQNDKNVFMAAEGSSQHGIEAPIRTACPPGIREGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRHHLQLPEPIAFNPYENASKLKRLPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEVEYHSKHQEAYKQLIHNY

Homology

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BLAST of Cp4.1LG10g04470 vs. Swiss-Prot
Match: FLUG_EMENI (Protein fluG OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) GN=fluG PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 5.7e-33
Identity = 129/428 (30.14%), Postives = 188/428 (43.93%), Query Frame = 1


Query: 27  VRIIW---VDGSGQHRCRAVPFKRFNDVT-KRNGIGLPFAVMAASSCNDCPAAGSNLSSV 86
           V+ +W   +D +   R R  P   F  +  K+  +G+  A        D    G   S+ 
Sbjct: 444 VKYVWTQFIDYTATVRVRMFPVMEFAKIVRKQRRLGISMATFWM--LQDDEVVGG--STT 503

Query: 87  GEMSLMPDLST-KRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVM 146
           G+  L+PDLST    V  + +   VM   +   GE+ E CPR  L  ++  LKDEF +  
Sbjct: 504 GQFYLIPDLSTLSPNVGIDSKSATVMTWWKSEQGESLEECPRTNLLNINNKLKDEFGIQA 563

Query: 147 KAGFEIEFLLLKKAV--RDGKEDWVPFDST--------------PYCSALSYDAASPFLH 206
             GFEIE + LK       G+EDW P  +               P    ++   AS  +H
Sbjct: 564 TCGFEIEVVFLKPTTDPSTGEEDWAPSVTNHSWSQMTRETRRMLPLLEEIAETLASIGIH 623

Query: 207 --ELHAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGS 266
             + HAE+  GQFE +L     + A D LI +++VI     KHGL AT  P+      G+
Sbjct: 624 LQQFHAESAPGQFEFILPPDNPVAAVDTLIKSRQVIANIVEKHGLRATLYPRPYPSAAGT 683

Query: 267 GSHVHVSLWQNDKN---------------VFMAAEGSSQHGIEAPIRTAC---------- 326
            SH HVS+  + K                 F  +  +S   +++ I              
Sbjct: 684 ASHAHVSISPSTKEESFLAGVLQHYPAVLAFTLSGDASYDRVKSGIWAGSEWVTWGTQNR 743

Query: 327 PPGIREGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRHHLQLPEPIAFNPYENASK-- 386
              IR+ S  ++EIKS DG AN YL +AA L+ G  G++ +  LP  I   PY+ AS   
Sbjct: 744 EAPIRKISPGHWEIKSLDGLANMYLAMAAFLAAGYTGVKEN--LPLTIKDCPYDAASLPE 803

Query: 387 --------LKRLPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEVEYHSKHQE--A 395
                     +LP +LA+S+ ALE +  L  L+GE LV     V++AE +  S   E   
Sbjct: 804 SERAALGITTKLPNTLAKSLAALESDEILRSLLGENLVEDYIIVKRAESKKLSAMDEKAR 863

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BLAST of Cp4.1LG10g04470 vs. Swiss-Prot
Match: GLNA_STAES (Glutamine synthetase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=glnA PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 108/366 (29.51%), Postives = 154/366 (42.08%), Query Frame = 1


Query: 84  EMSLMPDLSTKRTVPWNKQEEMV---MGDMQLRPGEAWEYCPREALRRVSRILKD----E 143
           +M L PDL T    PW   +  V   + D+    G  +E  PR  L+RV R ++D    +
Sbjct: 69  DMYLHPDLDTWVIFPWTAGQGKVARLICDVFKTDGTPFEGDPRANLKRVLRRMEDMGFTD 128

Query: 144 FNLVMKAGFEIEFLLLKKAVRD----------GKEDWVPFDSTPYCS---ALSYDAASPF 203
           FNL    G E EF L K   +           G  D  P D    C     L  +     
Sbjct: 129 FNL----GPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFD 188

Query: 204 LHELHAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGS 263
           +   H E   GQ EI   Y   + A DN+   + V+K  ARKH L ATF+PK      GS
Sbjct: 189 IEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGVNGS 248

Query: 264 GSHVHVSLWQNDKNVFMAAEGSSQ----------------------------------HG 323
           G H +VSL++  +N F   EG  Q                                   G
Sbjct: 249 GMHFNVSLFKGKENAFFDPEGDLQLTDTAYQFTAGVLKNARGFTAVCNPIVNSYKRLVPG 308

Query: 324 IEAPIRTACP-----PGIR----EGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRHHL 381
            EAP   A       P +R     G  +  E++S D  ANPY+ LAAIL  GLDG+ + L
Sbjct: 309 YEAPCYIAWSGKNRSPLVRVPTSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIENKL 368

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BLAST of Cp4.1LG10g04470 vs. Swiss-Prot
Match: GLNA_STAEQ (Glutamine synthetase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=glnA PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 108/366 (29.51%), Postives = 154/366 (42.08%), Query Frame = 1


Query: 84  EMSLMPDLSTKRTVPWNKQEEMV---MGDMQLRPGEAWEYCPREALRRVSRILKD----E 143
           +M L PDL T    PW   +  V   + D+    G  +E  PR  L+RV R ++D    +
Sbjct: 69  DMYLHPDLDTWVIFPWTAGQGKVARLICDVFKTDGTPFEGDPRANLKRVLRRMEDMGFTD 128

Query: 144 FNLVMKAGFEIEFLLLKKAVRD----------GKEDWVPFDSTPYCS---ALSYDAASPF 203
           FNL    G E EF L K   +           G  D  P D    C     L  +     
Sbjct: 129 FNL----GPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFD 188

Query: 204 LHELHAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGS 263
           +   H E   GQ EI   Y   + A DN+   + V+K  ARKH L ATF+PK      GS
Sbjct: 189 IEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGVNGS 248

Query: 264 GSHVHVSLWQNDKNVFMAAEGSSQ----------------------------------HG 323
           G H +VSL++  +N F   EG  Q                                   G
Sbjct: 249 GMHFNVSLFKGKENAFFDPEGDLQLTDTAYQFTAGVLKNARGFTAVCNPIVNSYKRLVPG 308

Query: 324 IEAPIRTACP-----PGIR----EGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRHHL 381
            EAP   A       P +R     G  +  E++S D  ANPY+ LAAIL  GLDG+ + L
Sbjct: 309 YEAPCYIAWSGKNRSPLVRVPTSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIENKL 368

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BLAST of Cp4.1LG10g04470 vs. Swiss-Prot
Match: GLNA_PYRFU (Glutamine synthetase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) GN=glnA PE=3 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 5.2e-26
Identity = 112/427 (26.23%), Postives = 169/427 (39.58%), Query Frame = 1


Query: 25  KLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSSVGE 84
           K V++++VD +G  +   +P  R  +           AV    S +     G       +
Sbjct: 15  KFVQLVFVDINGMPKGMEIPASRLEE-----------AVTDGISFDGSSVPGFQGIEDSD 74

Query: 85  MSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVMKAG 144
           +    D  T   VPW+    +       +  + +   PR  L+R    L+ E       G
Sbjct: 75  LVFKADPDTYVEVPWDNVARVY--GFIYKDNKPYGADPRGILKRALEELEKE-GYKAYIG 134

Query: 145 FEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHE--------------LHAE 204
            E EF L KK   +G  +    D   Y   L+ D A     E              LH E
Sbjct: 135 PEPEFYLFKK---NGTWELEIPDVGGYFDILTLDKARDIRREIAEYMPSFGLIPEVLHHE 194

Query: 205 AGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHVHVS 264
            GK Q EI   Y   L  ADN++  + + KA A  HGL ATF+PK      G+G H+H+S
Sbjct: 195 VGKAQHEIDFRYDEALKTADNIVSFKYITKAVAEMHGLYATFMPKPLFGFPGNGMHLHIS 254

Query: 265 LWQNDKNVFMAAEGSSQHGI-------------------------------EAPIRTACP 324
           LW++ +NVFM  EG S+  +                               EAP+  +  
Sbjct: 255 LWKDGENVFMGEEGLSEIALHFIGGILKHAKALTAVTNPTVNSYKRLVPSYEAPVYISWG 314

Query: 325 PGIRE---------GSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRHHLQLPEPIAFNP 384
              R          G  +  E +  D  ANPY   AA+L  GLDG++H +     +  N 
Sbjct: 315 YRNRSALIRVPAFWGKGARIEYRCPDPSANPYFAFAAVLKAGLDGIKHKIDPFAYVEENV 374

Query: 385 YENASK------LKRLPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEVEYHSKHQ 392
           YE + +      ++ LP SL E++E LEK+  + E +G+          K  + Y  K  
Sbjct: 375 YEMSEEKRKELGIETLPGSLGEALEELEKDKVVKEALGD--------AYKNFINYKWKEW 416

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BLAST of Cp4.1LG10g04470 vs. Swiss-Prot
Match: GLNA_METVO (Glutamine synthetase OS=Methanococcus voltae GN=glnA PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 6.8e-26
Identity = 100/377 (26.53%), Postives = 158/377 (41.91%), Query Frame = 1


Query: 84  EMSLMPDLSTKRTVPWNKQEEMV---MGDMQLRPGEAWEYCPREALRRVSRILKDEFNLV 143
           +M L PDLST   +PW   E+ V   + D+  + G+ +E  PR  L+RV    K+EF   
Sbjct: 73  DMVLKPDLSTLSVLPWRPSEKSVARIICDVYRKNGKPFEGDPRGCLKRVLAEFKEEFKGE 132

Query: 144 MKAGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHEL------------- 203
              G E EF +LK    +GK  WVP D   Y      D  +     +             
Sbjct: 133 YFVGPEPEFFILKN--ENGK--WVPGDDAGYFELEPLDEGNDLRRNIVFALENLGFHVEA 192

Query: 204 -HAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSH 263
            H E   GQ E+   Y   +  AD++I  +  IK  A++ G+LATF+PK      GSG H
Sbjct: 193 SHHEVAPGQHEVDFKYDNAVKTADSVITFKTTIKTLAKQSGVLATFMPKPFFGMNGSGMH 252

Query: 264 VHVSLW-------QNDKNVFMAAE-------GSSQH--------------------GIEA 323
            + S+W        ++ N    ++       G  +H                    G EA
Sbjct: 253 CNQSIWLDGKPSFYDENNAHQLSDICLSYIGGILEHTKALVSVTNPTVNSYKRLVPGYEA 312

Query: 324 PIRTACPPGIR---------EGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRHHLQLP 383
           P+  A     R          G  +  E ++ D   NPYL    +L+ GLDG+R  L  P
Sbjct: 313 PVNIAWANSNRTSIIRVPAARGKGTRVEFRAPDPACNPYLAFTVMLAAGLDGVRRKLIAP 372

Query: 384 EPIAFNPYENASKLKR------LPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEV 395
           EP+  N +  +   KR      +P +L +++E L ++  L + +G+ +           +
Sbjct: 373 EPVEKNIFAMSEAEKREANIDSVPSNLYDAIEELRQDKVLKKALGDHIF-------NKFI 432

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BLAST of Cp4.1LG10g04470 vs. TrEMBL
Match: A0A0A0KKB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G448150 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 3.4e-141
Identity = 266/436 (61.01%), Postives = 304/436 (69.72%), Query Frame = 1


Query: 22  NDAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSS 81
           N  K+VRIIWVD SGQHRCR +P  RFNDV K+NG+GL    MA SS  + P  G +L+ 
Sbjct: 13  NQTKVVRIIWVDTSGQHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSHTEIPI-GKDLTG 72

Query: 82  VGEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVM 141
           VGE+ L+PDLSTKR VPW +QEEMVMGDM +RPGEAWEYCPRE LRR+S ILK+EF+L M
Sbjct: 73  VGEIRLLPDLSTKRIVPWMEQEEMVMGDMYIRPGEAWEYCPRETLRRLSSILKNEFDLEM 132

Query: 142 KAGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHE--------------L 201
           KAGFEIEFLLLKKAV+DGKEDWVPFDS+ YCS  SYDAA+PF H+              L
Sbjct: 133 KAGFEIEFLLLKKAVKDGKEDWVPFDSSSYCSTFSYDAAAPFFHDIVDCLNSLNITVEQL 192

Query: 202 HAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHV 261
           HAEA +GQFE  LGYT CL AAD LIYT+EVIKA ARKHGLLATF+PKYAMDDVGSG HV
Sbjct: 193 HAEAAEGQFEFALGYTTCLTAADELIYTREVIKAVARKHGLLATFIPKYAMDDVGSGCHV 252

Query: 262 HVSLWQNDKNV--------------------------------FMAAEGSSQHGI----- 321
           H+SLWQN KNV                                F+A   +S   I     
Sbjct: 253 HISLWQNGKNVLMAADKSSKHGMSTIGEEFMAGVLYHIPSILPFIAPLPNSYDRIQPNTL 312

Query: 322 ------------EAPIRTACPPGIREGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRH 381
                       EAPIRTA PPGI    VSNFE+KSFDG ANPYLGLA+IL  GLDGLR+
Sbjct: 313 CGAYHCWGKDNREAPIRTASPPGIGVNLVSNFEVKSFDGLANPYLGLASILCAGLDGLRN 372

Query: 382 HLQLPEPIAFNPYENASKLKRLPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEVE 395
           H+QLP+PI  NP     K +RLPQSL+E++EALE NN  T LIGE+L+ AIK +RKAEV 
Sbjct: 373 HIQLPQPIDTNPSYMDLKFQRLPQSLSEALEALENNNMFTNLIGERLLTAIKEIRKAEVN 432

[Close]

BLAST of Cp4.1LG10g04470 vs. TrEMBL
Match: A0A0D2SGQ1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G412500 PE=3 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.3e-129
Identity = 238/404 (58.91%), Postives = 288/404 (71.29%), Query Frame = 1


Query: 6   LKKEVDEAVLVDAHAP-NDAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVM 65
           LK     + ++  + P +   LVRI+W D SGQHRCR VP KRFNDV ++NG+GL FA M
Sbjct: 389 LKASDSPSYVIGTNVPEHSVSLVRILWADASGQHRCRVVPKKRFNDVVRKNGVGLTFACM 448

Query: 66  AASSCNDCPAAGSNLSSVGEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPRE 125
           A SS  D PA  +NL+  GE+ LMPDLST R +PW KQEEMV+ DM L+PG+AWEYCPRE
Sbjct: 449 AMSSAVDGPADETNLTGTGEIRLMPDLSTWREIPWKKQEEMVLADMHLKPGDAWEYCPRE 508

Query: 126 ALRRVSRILKDEFNLVMKAGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFL 185
           ALRRVS++LKDEFNLVM AGFE EF LLKK  R+GKE+WVP DS PYCS+  +DA S   
Sbjct: 509 ALRRVSKVLKDEFNLVMNAGFENEFYLLKKLEREGKEEWVPIDSKPYCSSSGFDAISTLF 568

Query: 186 HE--------------LHAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLA 245
            E              +HAEAG GQ+E+ LG+TAC  AADNLI+T+EV++A A KHGLLA
Sbjct: 569 QEIVAALNSLNVAVEQMHAEAGNGQYEMALGHTACTYAADNLIFTREVVRAIANKHGLLA 628

Query: 246 TFLPKYAMDDVGSGSHVHVSLWQNDKNVFMAAEGSSQHGIEAPIRTACPPGIREGSVSNF 305
           TF+P Y          +  + W        A +   +   EAP+RTACPPGI  G VSNF
Sbjct: 629 TFVPNY--------DRIQPNTWSG------AYQCWGKENREAPLRTACPPGIPNGFVSNF 688

Query: 306 EIKSFDGCANPYLGLAAILSGGLDGLRHHLQLPEPIAFNPYENASKLKRLPQSLAESVEA 365
           EIKSFDGCANP+LGLAAI++ G+DGLR HL LP+PI  NP     KL RLP+SL+ES+EA
Sbjct: 689 EIKSFDGCANPHLGLAAIIAAGIDGLRRHLHLPQPIDANPATLEGKLSRLPKSLSESLEA 748

Query: 366 LEKNNTLTELIGEKLVVAIKAVRKAEVEYHSKHQEAYKQLIHNY 395
           L+K+N L ELIGEKLVVAI  VRKAE+EY+SK++EAYKQLIH Y
Sbjct: 749 LQKDNVLKELIGEKLVVAISGVRKAEIEYYSKNKEAYKQLIHRY 778

[Close]

BLAST of Cp4.1LG10g04470 vs. TrEMBL
Match: A0A103Y8S6_CYNCS (Amidohydrolase 2 OS=Cynara cardunculus var. scolymus GN=Ccrd_017063 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.8e-126
Identity = 227/394 (57.61%), Postives = 284/394 (72.08%), Query Frame = 1


Query: 43  VPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSSVGEMSLMPDLSTKRTVPWNKQ 102
           VP KRF++  K NG+GL  A M  +S  DCPA G+NL+  GE+ L+PDLST+  +PW  Q
Sbjct: 472 VPQKRFDNFVKENGLGLTCAAMGLTSAMDCPADGTNLTGTGEIRLIPDLSTRCRIPWVMQ 531

Query: 103 EEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVMKAGFEIEFLLLKKAVRDGKED 162
           EEMV+ DM  +PG+ WEYCPRE LRRVS++LK+EFNLVM AGFE EF LLK  +RDG E+
Sbjct: 532 EEMVLADMHSKPGQVWEYCPRETLRRVSKVLKEEFNLVMNAGFENEFYLLKSCLRDGHEE 591

Query: 163 WVPFDSTPYCSALSYDAASPFLHE--------------LHAEAGKGQFEIVLGYTACLNA 222
           WVPFDS+ YC+  ++D ASP  HE              LHAEAG GQFEI L YT C NA
Sbjct: 592 WVPFDSSRYCATSAFDLASPIFHEVVNALQFLNIDAEQLHAEAGNGQFEIALRYTTCTNA 651

Query: 223 ADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHVHVSLWQNDKNVFMAAEGSSQH 282
           ADNLIY +EVI+A ARKHGLLATF+PKY +DD+GSGSHVH+SL QN +NVF A++G+S+H
Sbjct: 652 ADNLIYAREVIRAVARKHGLLATFVPKYTLDDIGSGSHVHISLSQNGQNVFAASDGASRH 711

Query: 283 GI----------------------------EAPIRTACPPGIREGSVSNFEIKSFDGCAN 342
           G+                            EAP+RTACPPG+ +G VSNFEIK+FDGCAN
Sbjct: 712 GMSRTGEEFMAGVLHHLPSLLAFTAPIPNREAPLRTACPPGVADGIVSNFEIKAFDGCAN 771

Query: 343 PYLGLAAILSGGLDGLRHHLQLPEPIAFNPYENASKLKRLPQSLAESVEALEKNNTLTEL 395
           P+L LAAI++ G+DGLR+HL LP+PI  NP     KL+RLP SLAESV+AL  +  L  L
Sbjct: 772 PHLALAAIIASGIDGLRNHLSLPDPIDENPDILRDKLQRLPVSLAESVKALVNDFALESL 831

[Close]

BLAST of Cp4.1LG10g04470 vs. TrEMBL
Match: B9H982_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s12280g PE=3 SV=2)

HSP 1 Score: 419.9 bits (1078), Expect = 3.5e-114
Identity = 222/437 (50.80%), Postives = 287/437 (65.68%), Query Frame = 1


Query: 22  NDAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSS 81
           N + LVR++WVD SGQHRCRAVP KRF+D+ ++NG+GL  A M  SS  D P+  + L+ 
Sbjct: 396 NSSSLVRVMWVDTSGQHRCRAVPVKRFSDIVRKNGVGLTHASMGMSSAADSPSDETGLTG 455

Query: 82  VGEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVM 141
           VGE+ L+PD++T++ +PW +++EMV+ DM LRPGE WEYCPREALRRV ++LKDEF+LVM
Sbjct: 456 VGEIRLIPDVTTRKKIPWMERQEMVLADMHLRPGEPWEYCPREALRRVLKVLKDEFDLVM 515

Query: 142 KAGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHE--------------L 201
            AGFE EF+LLK    +GKE+WVP DS PYCS  S+D  SP L E              L
Sbjct: 516 DAGFENEFVLLKSVSWEGKEEWVPIDSAPYCSTASFDLVSPILDEIVGALHSLDITVEQL 575

Query: 202 HAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHV 261
           HAE+GKGQFE+ +G+T C  +ADNLIYT+EVI+A ARKHGLLATF+PK A+DD+GSGSHV
Sbjct: 576 HAESGKGQFELAMGHTTCPLSADNLIYTREVIRAIARKHGLLATFVPKLALDDIGSGSHV 635

Query: 262 HVSLWQNDKNVFMAAEGSSQHGIEA-------------PIRTACPPGIREGSVSNFEIKS 321
           H+SL +N +NVFMA+ GSS+HGI +             P   A    I   S    +  +
Sbjct: 636 HISLLRNGENVFMASGGSSKHGISSIGEEFMAGVLHHLPSILAFTAPI-PNSYDRIQPNT 695

Query: 322 FDGCANPY-------------------------------------LGLAAILSGGLDGLR 381
           + G    +                                     LGLAAI + G+DGLR
Sbjct: 696 WSGAYQCWGKENREAPLRTACPPGIIDGLVSNFEIKSFDACANPYLGLAAIGAAGIDGLR 755

Query: 382 HHLQLPEPIAFNPYENASKLKRLPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEV 395
            HL+LPEPI  NP    + L RLPQSL ES+EAL+K++ L +L GEKL+VAIK VRKAE+
Sbjct: 756 KHLRLPEPIDKNP-SLCANLPRLPQSLPESLEALKKDSVLEDLFGEKLLVAIKGVRKAEI 815

[Close]

BLAST of Cp4.1LG10g04470 vs. TrEMBL
Match: A0A0A0KHB2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G448130 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 3.9e-113
Identity = 200/264 (75.76%), Postives = 222/264 (84.09%), Query Frame = 1


Query: 23  DAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSSV 82
           D KLVRIIWVDGSGQ RCRAVPFKRFNDV KR G+GL  A MA +S  DC A GSNLSSV
Sbjct: 407 DVKLVRIIWVDGSGQQRCRAVPFKRFNDVVKRTGVGLACAAMAMTSYADCTAKGSNLSSV 466

Query: 83  GEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVMK 142
           GE+ L+PDLST+  VPWNKQEEMV+GDMQ+RPGEAWEYCPREALRRV RILKDEF+LV+ 
Sbjct: 467 GEIRLLPDLSTRVAVPWNKQEEMVLGDMQVRPGEAWEYCPREALRRVCRILKDEFDLVLN 526

Query: 143 AGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHE--------------LH 202
           AGFE EF LLKKAVR G+EDWVPFDS PYCS  SYDAASPFLHE              +H
Sbjct: 527 AGFENEFFLLKKAVRHGEEDWVPFDSVPYCSTSSYDAASPFLHEVVDSLSSLNITVEQVH 586

Query: 203 AEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHVH 262
           AEAGKGQFEI LG+T CLNAADNL+YT+EVI+ATARKHGLLATF+PKY +DD+GSGSHVH
Sbjct: 587 AEAGKGQFEISLGHTVCLNAADNLVYTREVIRATARKHGLLATFIPKYDLDDIGSGSHVH 646

Query: 263 VSLWQNDKNVFMAAEGSSQHGIEA 273
           VSLWQN KNVFMA++GSS+HG+ A
Sbjct: 647 VSLWQNGKNVFMASDGSSKHGMSA 670

[Close]

BLAST of Cp4.1LG10g04470 vs. TAIR10
Match: AT3G53180.1 (AT3G53180.1 glutamate-ammonia ligases;catalytics;glutamate-ammonia ligases)

HSP 1 Score: 333.2 bits (853), Expect = 2.2e-91
Identity = 158/265 (59.62%), Postives = 198/265 (74.72%), Query Frame = 1


Query: 22  NDAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSS 81
           + +  VRIIWVD SGQ RCRAV  +RFN   K+NG+GL FA M  +S  D PA  S L+ 
Sbjct: 417 DSSSFVRIIWVDTSGQQRCRAVQAQRFNRSVKKNGVGLTFASMGMTSFTDGPAEESKLTG 476

Query: 82  VGEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVM 141
           VGE+ L+PDLSTK+T+PW KQE MV+ DMQL+PGEAW YCPRE LRRV+++LKDEF+LVM
Sbjct: 477 VGEIRLVPDLSTKQTIPWTKQESMVLADMQLKPGEAWGYCPRETLRRVAKVLKDEFDLVM 536

Query: 142 KAGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHEL-------------- 201
            AGFE EF LLK  VR+GKE+++PFD  PYC+  S+DAASP  H++              
Sbjct: 537 NAGFENEFYLLKNVVREGKEEYMPFDFGPYCATSSFDAASPIFHDIVPALESLNIEVEQF 596

Query: 202 HAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHV 261
           HAE+GKGQFE+ LG+T   +AADNL+YT+EVI++ ARK GLLATF+PKY   D+GSGSHV
Sbjct: 597 HAESGKGQFEVSLGHTIASHAADNLVYTREVIRSVARKQGLLATFVPKYDYCDIGSGSHV 656

Query: 262 HVSLWQNDKNVFMAAEGSSQHGIEA 273
           H+SLW+N +NVF A+  SS HGI +
Sbjct: 657 HLSLWKNGENVFPASNNSSSHGISS 681

[Close]

BLAST of Cp4.1LG10g04470 vs. NCBI nr
Match: gi|659125091|ref|XP_008462507.1| (PREDICTED: type-1 glutamine synthetase 1-like [Cucumis melo])

HSP 1 Score: 520.0 bits (1338), Expect = 3.6e-144
Identity = 271/435 (62.30%), Postives = 309/435 (71.03%), Query Frame = 1


Query: 23  DAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSSV 82
           + KLVRIIWVD SGQHRCR +P  RFNDV K+NGIGL    MA SS  D P  G NL+  
Sbjct: 21  EVKLVRIIWVDASGQHRCRVIPSTRFNDVVKKNGIGLSVVCMARSSHTDVPI-GKNLTGA 80

Query: 83  GEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVMK 142
           GE+ L+PDLSTKRTVPW +QEEMVMGDM ++PGEAWEYCPREALRRV  +LK+EF+L MK
Sbjct: 81  GEIRLLPDLSTKRTVPWMEQEEMVMGDMYIKPGEAWEYCPREALRRVFTVLKNEFDLEMK 140

Query: 143 AGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHE--------------LH 202
           AGFEIEFLLLKKAV+DGKEDWVPFDS+ YCS  SYDAA+PFLH+              LH
Sbjct: 141 AGFEIEFLLLKKAVKDGKEDWVPFDSSLYCSTSSYDAAAPFLHDVVDSLNSLNITVEQLH 200

Query: 203 AEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHVH 262
           AEAGKGQFE  LGYT CL AAD+L+YT+EVIKA ARKHGLLATF+PKYAMDDVGSG HVH
Sbjct: 201 AEAGKGQFEFALGYTTCLTAADDLVYTREVIKAVARKHGLLATFIPKYAMDDVGSGCHVH 260

Query: 263 VSLWQNDKNV--------------------------------FMAAEGSSQHGI------ 322
           +SLWQN KNV                                F+A   +S   I      
Sbjct: 261 ISLWQNGKNVLMATDKSSKHGMSTIGEEFMAGVLYHIPSILPFIAPLPNSYDRIQPNTFS 320

Query: 323 -----------EAPIRTACPPGIREGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRHH 382
                      E PIRTA  PGI    VSNFE+KSFDGCANPYLGLAAIL  GLDGLR+H
Sbjct: 321 GAYHCWGKDNREVPIRTASSPGIGVTLVSNFEVKSFDGCANPYLGLAAILCAGLDGLRYH 380

Query: 383 LQLPEPIAFNPYENASKLKRLPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEVEY 395
           LQLP+PI  NP     K +RLPQSL+E++E LEKNNT T+LIGE+L+ AIK +RKAEV+Y
Sbjct: 381 LQLPQPIDTNPSYMGLKFQRLPQSLSEALEHLEKNNTFTDLIGERLLAAIKEIRKAEVKY 440

[Close]

BLAST of Cp4.1LG10g04470 vs. NCBI nr
Match: gi|470137471|ref|XP_004304487.1| (PREDICTED: protein fluG [Fragaria vesca subsp. vesca])

HSP 1 Score: 514.2 bits (1323), Expect = 2.0e-142
Identity = 257/436 (58.94%), Postives = 309/436 (70.87%), Query Frame = 1


Query: 22  NDAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSS 81
           N    VR+ W D SGQ RCR VP KRFNDV  +NGIGL FA M  +S  D PA  +NL+ 
Sbjct: 407 NHVSFVRVFWADASGQQRCRVVPAKRFNDVVTKNGIGLTFACMGMTSFTDGPADETNLTG 466

Query: 82  VGEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVM 141
           VGE+ LMPDLSTK  +PW +QEEMV+ +M L+PGEAWEYCPRE L+RVS+ILKDEFNL M
Sbjct: 467 VGEIRLMPDLSTKWRIPWVEQEEMVLANMHLKPGEAWEYCPRETLQRVSKILKDEFNLEM 526

Query: 142 KAGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLH--------------EL 201
            AGFE EF LLK  +RDGKE+WVPFDSTPYCS  SYDAASP  H              +L
Sbjct: 527 NAGFENEFFLLKSTLRDGKEEWVPFDSTPYCSPSSYDAASPLFHKVIAALQSLNITVEQL 586

Query: 202 HAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHV 261
           HAE+GKGQFE+ LG+TACL+AADNLIYT+EVI+A ARKHGLLATF+PKYA+D++GSG+HV
Sbjct: 587 HAESGKGQFEMALGHTACLHAADNLIYTREVIRAIARKHGLLATFMPKYALDEIGSGAHV 646

Query: 262 HVSLWQNDKNVFMAAEGSSQHGIE------------------------------------ 321
           H+SLWQN KNVFMA+ GSSQHG+                                     
Sbjct: 647 HLSLWQNGKNVFMASGGSSQHGMSKVGEEFMAGVLYHLPAVLAFTAPIPNSYDRIQPNTW 706

Query: 322 -------------APIRTACPPGIREGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRH 381
                        AP+RTACPPGI+ G VSNFEIKSFDGCANP+LGLAAIL+ G+DGLR 
Sbjct: 707 SGAYKCWGKENREAPLRTACPPGIQSGLVSNFEIKSFDGCANPHLGLAAILAAGIDGLRR 766

Query: 382 HLQLPEPIAFNPYENASKLKRLPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEVE 395
            L LP+PI  NP     +L+RLP+SL+ES+EAL++++   + IGEKL+VAIK VRKAE++
Sbjct: 767 QLCLPKPIDTNPSSLEGELQRLPKSLSESLEALKEDDLFKDFIGEKLLVAIKGVRKAEID 826

[Close]

BLAST of Cp4.1LG10g04470 vs. NCBI nr
Match: gi|778717284|ref|XP_011657677.1| (PREDICTED: type-1 glutamine synthetase 1-like isoform X1 [Cucumis sativus])

HSP 1 Score: 509.6 bits (1311), Expect = 4.9e-141
Identity = 266/436 (61.01%), Postives = 304/436 (69.72%), Query Frame = 1


Query: 22  NDAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSS 81
           N  K+VRIIWVD SGQHRCR +P  RFNDV K+NG+GL    MA SS  + P  G +L+ 
Sbjct: 13  NQTKVVRIIWVDTSGQHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSHTEIPI-GKDLTG 72

Query: 82  VGEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVM 141
           VGE+ L+PDLSTKR VPW +QEEMVMGDM +RPGEAWEYCPRE LRR+S ILK+EF+L M
Sbjct: 73  VGEIRLLPDLSTKRIVPWMEQEEMVMGDMYIRPGEAWEYCPRETLRRLSSILKNEFDLEM 132

Query: 142 KAGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHE--------------L 201
           KAGFEIEFLLLKKAV+DGKEDWVPFDS+ YCS  SYDAA+PF H+              L
Sbjct: 133 KAGFEIEFLLLKKAVKDGKEDWVPFDSSSYCSTFSYDAAAPFFHDIVDCLNSLNITVEQL 192

Query: 202 HAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHV 261
           HAEA +GQFE  LGYT CL AAD LIYT+EVIKA ARKHGLLATF+PKYAMDDVGSG HV
Sbjct: 193 HAEAAEGQFEFALGYTTCLTAADELIYTREVIKAVARKHGLLATFIPKYAMDDVGSGCHV 252

Query: 262 HVSLWQNDKNV--------------------------------FMAAEGSSQHGI----- 321
           H+SLWQN KNV                                F+A   +S   I     
Sbjct: 253 HISLWQNGKNVLMAADKSSKHGMSTIGEEFMAGVLYHIPSILPFIAPLPNSYDRIQPNTL 312

Query: 322 ------------EAPIRTACPPGIREGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRH 381
                       EAPIRTA PPGI    VSNFE+KSFDG ANPYLGLA+IL  GLDGLR+
Sbjct: 313 CGAYHCWGKDNREAPIRTASPPGIGVNLVSNFEVKSFDGLANPYLGLASILCAGLDGLRN 372

Query: 382 HLQLPEPIAFNPYENASKLKRLPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEVE 395
           H+QLP+PI  NP     K +RLPQSL+E++EALE NN  T LIGE+L+ AIK +RKAEV 
Sbjct: 373 HIQLPQPIDTNPSYMDLKFQRLPQSLSEALEALENNNMFTNLIGERLLTAIKEIRKAEVN 432

[Close]

BLAST of Cp4.1LG10g04470 vs. NCBI nr
Match: gi|778717287|ref|XP_011657678.1| (PREDICTED: type-1 glutamine synthetase 1-like isoform X2 [Cucumis sativus])

HSP 1 Score: 478.0 bits (1229), Expect = 1.6e-131
Identity = 251/425 (59.06%), Postives = 292/425 (68.71%), Query Frame = 1


Query: 33  DGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVMAASSCNDCPAAGSNLSSVGEMSLMPDLS 92
           +  G ++ + +P  RFNDV K+NG+GL    MA SS  + P  G +L+ VGE+ L+PDLS
Sbjct: 8   ENGGVNQTKVIPSTRFNDVVKKNGVGLSVVCMARSSHTEIPI-GKDLTGVGEIRLLPDLS 67

Query: 93  TKRTVPWNKQEEMVMGDMQLRPGEAWEYCPREALRRVSRILKDEFNLVMKAGFEIEFLLL 152
           TKR VPW +QEEMVMGDM +RPGEAWEYCPRE LRR+S ILK+EF+L MKAGFEIEFLLL
Sbjct: 68  TKRIVPWMEQEEMVMGDMYIRPGEAWEYCPRETLRRLSSILKNEFDLEMKAGFEIEFLLL 127

Query: 153 KKAVRDGKEDWVPFDSTPYCSALSYDAASPFLHE--------------LHAEAGKGQFEI 212
           KKAV+DGKEDWVPFDS+ YCS  SYDAA+PF H+              LHAEA +GQFE 
Sbjct: 128 KKAVKDGKEDWVPFDSSSYCSTFSYDAAAPFFHDIVDCLNSLNITVEQLHAEAAEGQFEF 187

Query: 213 VLGYTACLNAADNLIYTQEVIKATARKHGLLATFLPKYAMDDVGSGSHVHVSLWQNDKNV 272
            LGYT CL AAD LIYT+EVIKA ARKHGLLATF+PKYAMDDVGSG HVH+SLWQN KNV
Sbjct: 188 ALGYTTCLTAADELIYTREVIKAVARKHGLLATFIPKYAMDDVGSGCHVHISLWQNGKNV 247

Query: 273 --------------------------------FMAAEGSSQHGI---------------- 332
                                           F+A   +S   I                
Sbjct: 248 LMAADKSSKHGMSTIGEEFMAGVLYHIPSILPFIAPLPNSYDRIQPNTLCGAYHCWGKDN 307

Query: 333 -EAPIRTACPPGIREGSVSNFEIKSFDGCANPYLGLAAILSGGLDGLRHHLQLPEPIAFN 392
            EAPIRTA PPGI    VSNFE+KSFDG ANPYLGLA+IL  GLDGLR+H+QLP+PI  N
Sbjct: 308 REAPIRTASPPGIGVNLVSNFEVKSFDGLANPYLGLASILCAGLDGLRNHIQLPQPIDTN 367

Query: 393 PYENASKLKRLPQSLAESVEALEKNNTLTELIGEKLVVAIKAVRKAEVEYHSKHQEAYKQ 395
           P     K +RLPQSL+E++EALE NN  T LIGE+L+ AIK +RKAEV ++SKHQEAYKQ
Sbjct: 368 PSYMDLKFQRLPQSLSEALEALENNNMFTNLIGERLLTAIKEIRKAEVNHYSKHQEAYKQ 427

[Close]

BLAST of Cp4.1LG10g04470 vs. NCBI nr
Match: gi|763795337|gb|KJB62333.1| (hypothetical protein B456_009G412500 [Gossypium raimondii])

HSP 1 Score: 471.1 bits (1211), Expect = 1.9e-129
Identity = 238/404 (58.91%), Postives = 288/404 (71.29%), Query Frame = 1


Query: 6   LKKEVDEAVLVDAHAP-NDAKLVRIIWVDGSGQHRCRAVPFKRFNDVTKRNGIGLPFAVM 65
           LK     + ++  + P +   LVRI+W D SGQHRCR VP KRFNDV ++NG+GL FA M
Sbjct: 389 LKASDSPSYVIGTNVPEHSVSLVRILWADASGQHRCRVVPKKRFNDVVRKNGVGLTFACM 448

Query: 66  AASSCNDCPAAGSNLSSVGEMSLMPDLSTKRTVPWNKQEEMVMGDMQLRPGEAWEYCPRE 125
           A SS  D PA  +NL+  GE+ LMPDLST R +PW KQEEMV+ DM L+PG+AWEYCPRE
Sbjct: 449 AMSSAVDGPADETNLTGTGEIRLMPDLSTWREIPWKKQEEMVLADMHLKPGDAWEYCPRE 508

Query: 126 ALRRVSRILKDEFNLVMKAGFEIEFLLLKKAVRDGKEDWVPFDSTPYCSALSYDAASPFL 185
           ALRRVS++LKDEFNLVM AGFE EF LLKK  R+GKE+WVP DS PYCS+  +DA S   
Sbjct: 509 ALRRVSKVLKDEFNLVMNAGFENEFYLLKKLEREGKEEWVPIDSKPYCSSSGFDAISTLF 568

Query: 186 HE--------------LHAEAGKGQFEIVLGYTACLNAADNLIYTQEVIKATARKHGLLA 245
            E              +HAEAG GQ+E+ LG+TAC  AADNLI+T+EV++A A KHGLLA
Sbjct: 569 QEIVAALNSLNVAVEQMHAEAGNGQYEMALGHTACTYAADNLIFTREVVRAIANKHGLLA 628

Query: 246 TFLPKYAMDDVGSGSHVHVSLWQNDKNVFMAAEGSSQHGIEAPIRTACPPGIREGSVSNF 305
           TF+P Y          +  + W        A +   +   EAP+RTACPPGI  G VSNF
Sbjct: 629 TFVPNY--------DRIQPNTWSG------AYQCWGKENREAPLRTACPPGIPNGFVSNF 688

Query: 306 EIKSFDGCANPYLGLAAILSGGLDGLRHHLQLPEPIAFNPYENASKLKRLPQSLAESVEA 365
           EIKSFDGCANP+LGLAAI++ G+DGLR HL LP+PI  NP     KL RLP+SL+ES+EA
Sbjct: 689 EIKSFDGCANPHLGLAAIIAAGIDGLRRHLHLPQPIDANPATLEGKLSRLPKSLSESLEA 748

Query: 366 LEKNNTLTELIGEKLVVAIKAVRKAEVEYHSKHQEAYKQLIHNY 395
           L+K+N L ELIGEKLVVAI  VRKAE+EY+SK++EAYKQLIH Y
Sbjct: 749 LQKDNVLKELIGEKLVVAISGVRKAEIEYYSKNKEAYKQLIHRY 778

The following BLAST results are available for this feature:

BLAST of Cp4.1LG10g04470 vs. Swiss-Prot


Match Name	E-value	Identity	Description
FLUG_EMENI	5.7e-33	30.14	Protein fluG OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / ...	[more]
GLNA_STAES	1.0e-26	29.51	Glutamine synthetase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=glnA P...	[more]
GLNA_STAEQ	1.0e-26	29.51	Glutamine synthetase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) G...	[more]
GLNA_PYRFU	5.2e-26	26.23	Glutamine synthetase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM ...	[more]
GLNA_METVO	6.8e-26	26.53	Glutamine synthetase OS=Methanococcus voltae GN=glnA PE=3 SV=1	[more]

BLAST of Cp4.1LG10g04470 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKB4_CUCSA	3.4e-141	61.01	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G448150 PE=3 SV=1	[more]
A0A0D2SGQ1_GOSRA	1.3e-129	58.91	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G412500 PE=3 SV=1	[more]
A0A103Y8S6_CYNCS	1.8e-126	57.61	Amidohydrolase 2 OS=Cynara cardunculus var. scolymus GN=Ccrd_017063 PE=3 SV=1	[more]
B9H982_POPTR	3.5e-114	50.80	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s12280g PE=3 SV=2	[more]
A0A0A0KHB2_CUCSA	3.9e-113	75.76	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G448130 PE=3 SV=1	[more]

BLAST of Cp4.1LG10g04470 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G53180.1	2.2e-91	59.62	glutamate-ammonia ligases;catalytics;glutamate-ammonia ligases	[more]

BLAST of Cp4.1LG10g04470 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659125091\|ref\|XP_008462507.1\|	3.6e-144	62.30	PREDICTED: type-1 glutamine synthetase 1-like [Cucumis melo]	[more]
gi\|470137471\|ref\|XP_004304487.1\|	2.0e-142	58.94	PREDICTED: protein fluG [Fragaria vesca subsp. vesca]	[more]
gi\|778717284\|ref\|XP_011657677.1\|	4.9e-141	61.01	PREDICTED: type-1 glutamine synthetase 1-like isoform X1 [Cucumis sativus]	[more]
gi\|778717287\|ref\|XP_011657678.1\|	1.6e-131	59.06	PREDICTED: type-1 glutamine synthetase 1-like isoform X2 [Cucumis sativus]	[more]
gi\|763795337\|gb\|KJB62333.1\|	1.9e-129	58.91	hypothetical protein B456_009G412500 [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0004356	glutamate-ammonia ligase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006542	glutamine biosynthetic process
GO:0006807	nitrogen compound metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR014746	Gln_synth/guanido_kin_cat_dom
IPR008147	Gln_synt_b-grasp
IPR008146	Gln_synth_cat_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006542	glutamine biosynthetic process
biological_process	GO:0009252	peptidoglycan biosynthetic process
biological_process	GO:0050896	response to stimulus
biological_process	GO:0006807	nitrogen compound metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004356	glutamate-ammonia ligase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG10g04470.1	Cp4.1LG10g04470.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008146	Glutamine synthetase, catalytic domain	PFAM	PF00120	Gln-synt_C	coord: 120..267 score: 1.7E-28coord: 274..385 score: 5.9
IPR008146	Glutamine synthetase, catalytic domain	SMART	SM01230	Gln_synt_C_2	coord: 119..312 score: 3.8
IPR008147	Glutamine synthetase, beta-Grasp domain	GENE3D	G3DSA:3.10.20.70		coord: 22..122 score: 9.
IPR008147	Glutamine synthetase, beta-Grasp domain	unknown	SSF54368	Glutamine synthetase, N-terminal domain	coord: 17..118 score: 2.75
IPR014746	Glutamine synthetase/guanido kinase, catalytic domain	GENE3D	G3DSA:3.30.590.10		coord: 123..264 score: 1.1E-31coord: 272..381 score: 1.2
None	No IPR available	PANTHER	PTHR20852	GLUTAMINE SYNTHETASE	coord: 22..394 score: 5.3E
None	No IPR available	PANTHER	PTHR20852:SF16	TYPE-1 GLUTAMINE SYNTHETASE 1	coord: 22..394 score: 5.3E
None	No IPR available	unknown	SSF55931	Glutamine synthetase/guanido kinase	coord: 119..387 score: 1.67

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG10g04470	Cucurbita maxima (Rimu)	cmacpeB254
Cp4.1LG10g04470	Cucurbita maxima (Rimu)	cmacpeB656
Cp4.1LG10g04470	Cucurbita maxima (Rimu)	cmacpeB866
Cp4.1LG10g04470	Cucurbita moschata (Rifu)	cmocpeB218
Cp4.1LG10g04470	Cucurbita moschata (Rifu)	cmocpeB606
Cp4.1LG10g04470	Cucurbita moschata (Rifu)	cmocpeB806
Cp4.1LG10g04470	Wild cucumber (PI 183967)	cpecpiB081
Cp4.1LG10g04470	Cucumber (Chinese Long) v2	cpecuB085
Cp4.1LG10g04470	Bottle gourd (USVL1VR-Ls)	cpelsiB054
Cp4.1LG10g04470	Watermelon (Charleston Gray)	cpewcgB071
Cp4.1LG10g04470	Watermelon (97103) v1	cpewmB078
Cp4.1LG10g04470	Melon (DHL92) v3.5.1	cpemeB069
Cp4.1LG10g04470	Cucumber (Gy14) v2	cgybcpeB291
Cp4.1LG10g04470	Cucumber (Gy14) v2	cgybcpeB710
Cp4.1LG10g04470	Melon (DHL92) v3.6.1	cpemedB069
Cp4.1LG10g04470	Melon (DHL92) v3.6.1	cpemedB072
Cp4.1LG10g04470	Melon (DHL92) v3.6.1	cpemedB090
Cp4.1LG10g04470	Silver-seed gourd	carcpeB0337
Cp4.1LG10g04470	Silver-seed gourd	carcpeB0348
Cp4.1LG10g04470	Silver-seed gourd	carcpeB0930
Cp4.1LG10g04470	Cucumber (Chinese Long) v3	cpecucB0070
Cp4.1LG10g04470	Cucumber (Chinese Long) v3	cpecucB0093
Cp4.1LG10g04470	Wax gourd	cpewgoB0067
Cp4.1LG10g04470	Wax gourd	cpewgoB0100
Cp4.1LG10g04470	Cucurbita pepo (Zucchini)	cpecpeB076
Cp4.1LG10g04470	Cucurbita pepo (Zucchini)	cpecpeB092
Cp4.1LG10g04470	Cucumber (Gy14) v1	cgycpeB0314