Cp4.1LG08g07970 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG08g07970
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionSesquiterpene synthase Tps3
LocationCp4.1LG08 : 6400080 .. 6403866 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAAAACAAAAAGAAGATACACCAGCCATGGCCTCAAAAGATAATACCTCACTCGATGATGGGGTTGTTCCTCGTCGATTTGCAAACTTTCCACCAAGTGCATGGGGAGATTTCTTCATTTCCTATGATTCCAACACTATGGTATTTCAAATATATTATTTTGTTGTTGTTTAAATTACTAATTTTGATTCAATCGTTCGTGTTTATTTCATTCTGATTCTACAGTGATATCGTTTATTTTTGTACAAATATTAAGGAAAATCAGCTCGAGGAGAAGATGGTCGAGAAACAGCTTCAAAAGCTTAAAGAAGAAGTGATGAACATGTTTGTGACAACAGACAAGTCTTCGGAAAAATTAAGGTTGATAGATTCAATTCAACGCTTAGGTTTGTCCCATCACTTTCAACTGGAAATTAATGAAACACTTCAACTTCTGCAAAGCTCTTCCGACGATGATGACGATATTTACAATATTGCCCTTAAGTTTCGATTGCTTAGACAAGAAGGTTATTCTATTTCATCTGGTAAGTCTCTAGCTCCACCATTTTTAAAATTTTTAGAAAAAAATGAAAATCGGATTTGATTCGTAAATTTTGAGCAGAGATATTCAACAAGTTTATAAATGAAGAGGGAGAATTCAAAGAATCAATTGTGAAAGACAAAAGTGGAATATTAAGTTTGTACGAGGCGTCCCATTTAAGAATGAACGGAGAGTATACATTAGATAAAGCTTTGAGGTTCACAACTACTCATCTTCAAGAAATGGCCATGGATGAGAGCTCTCCTTTAGTAGATGAAGCCAAGCATGCTTTGAAGTGGCCAATCTATAAAACTGTGCCAAGGCTTATGACCAAGCACTACATTTCTGTCCATCAGAAGGATCCACCAAACAGTGTTTTGCTCACTTTTGCAAAGTTGGATTACACCACATCTCAAAAGCTTTATCAGAAAGAACTCGGTCAACATGCGAGGTTTGTCGTAAATTTTTTTTTTTTGAGGTGTTCAAAATATTCGATAATCTGAAAATTTCGATCAATTTAACCCAATTCAATTCGGGTGGGCTGGATTATAAACGTATTTAAATTGAATTAAGTTCAAATAAATGAAAACTTTACGGTAATTCACAATTTCTCCTAAAACTATATTGAGTTGAATTGAGTCGGTTGATTTTAAAATATAATTTTTTTTTATTTTTTTTTATCATTTATTGTTTTAACCACTCTTAAAAATATATGGACCATAGATAAGTAAATATAGAAGGAATTAACATTTTACTTATATTAAAAATAAAAATTTGAACTTCGTCACCTTTATATAGTTCAAAGTTTAATTTAGGCGTTTGTGCGTAAATATGTAGGGTTAGAAATCACGACTCTCCACACTAGTATGATATTGTCTGTCCACTTTCAGCACAAGCACTCATGGCTTTGTTGGCTTTGTTTTGGGCTTCTCCAAAAAGCATCCTACCAATGGAGATAATATTCCTCACTTATAAATCCATGATCTTCACTAAATTAGCTAAACGTAGGACTGACTCTCAATAATCCTCAACATGTAGGTGGTGGAAGCAGTTGAATTTAATAGAAAGACTATCTTTTGCACGAGATCGGGCAGTGGAGAGCTACTTTTGGGCGTTGGGAGTGTACTATGAACCCAACTACTCGGTTGGTAGAATGATATTAGCCAAAATTATTGCTCTAGCAACGGTTCTGGATGATATGTACGATGTATATGCTACATTGGATGAACTTGACCTATTCACAGAGGCAATTGAAAGGTTTAAGCTCTTTTTAACTCTTCGTTATATTTGGTTCATGAACTAGCTCTTCTGAATTGTCGTAAAAATTTGGATCATGAATAATTGGATTTAAGAATAGTATATTCTCCACTTCGATTTCGAATTTATTGGATTCATTTGGTTTATCTCCCATATTTTCATGTATGAACGTTAATACTGATTTGAGAGAATTAAGATTGCTATATCTATATTAAAACTTGTCCAGCATTATTTTGAGACGTGAAGTCATTGTCATTTGTGTTTTCCTAAAGTCAATTGGGAGGACTTTTGTTAGAGATTTTGTAATGTGAGAGTGTGAGAGATAAACTTGTAAACATTTTGGTTACAGTGATTGGCTGCCACCCGTGGATATAGGAGAATTTTTTCACTCCAAACCACGTAAATTTTCGTGCCGCTTTGTTTAATTTCTATTCGTGATTGTGTTAGTGGGGTCGATGTAGTTTCCATTTCCGCTACAACAATTGATATCAGAGCATCGGATTGAATCTTATGCCTTTTGATTAAATTTCAGGTGGGATACTAACTGCATGGAGAAGCTCCCAGAGTACATGAAGATATTTTATGAAGTGATATTAAAAACCTATGAAGAGTTTGAGGAAGATATGAGCAAGGACATCATTAACTATGCCATTAATTATGCAAAAGAAGCGGTATGAGTTGTTGCACACTAATCTAATCTAATGTTCATCACAAATAATGGCTCTCTTTCTAATTCTTTGTTTTGGGAACAGTTTAAAAGGCAGTGTAGAGTTTATTTTATGGAAGCTAAATGGTACCATGAAGGATATGTGCCAAGCATGGAGGAGTACATGAAAGTGTCCATTGTATCCACTTGTTACTATCTTTTTGCTCCAATTTCATTTCTTGGTATGGGCGTTGCTGCTTCTGAAGAGGCTTTCAAATGGGTTGAGAGTGATCCAATGATGCTCAAGGCAAGTGGAATAATTGGGAGGCTCATGAATGACATAACTTCACACAAGGTACACAACCCATTTAAAGTAAAGGGGAGAATTAATATTAAGTTTTGTTCATATATTTAGCTTCATTCAAGCTCACCTAATGGTGGGTATTAATTAGTTTCGAAGTGAAAAGTAATCGTGTAATGACTCAAACCCACCGCTAGCAGATATTGTTCTTTTTGGACTTTTTCTTTTCGGGTCTTCGTTCACGGTTTTTAAAACGCGTAGGGAGAGGTTTCCACACCGTTATAAAGAATGTTTATTTCTCCTCCCCACCAATGTGGGATCTCACAATGAACTGATAGTAAGGGATCCCATATCGGTTGGAGAAGAGAATGAAACATTTCTTTTTATAAGGGTGTGGAAACCTTCTTAAAACCCTTGAGGGGAAGCCCAAAGAGGAAAGCTCATAGAGAACAATATTTGTTGCGGTGGGCTTGGACTGTTGCAAATGATATTAGAGCTAGACACCGGGTGATGTGCTAGTGAGGAGGCTGAGCCCCGAAAGGGGATAGACACGAGGTGGTGTGCTAGCAAGGACTCTGGGTCCCGAAGGGGGTAGATTGTGAGATCCTATATCGGTTGGGGAGGAGATCGAAACATTCTTTATAAGAATCTTGAGGAGAAGTGGGAAAGAAAAAGTCCAAAGAGGACGGCATCTATTAGGAATGGGCTTGAACCGTCACAAATCACTTGGAAGCCGTTTGGTAATCAATTTTTTTTCTCTTTGGTGATTAGTTTGACCAAGAGAGAGGGGACGTTGGGTCAGCGGTTGAATGTTACATGAAGCAACATGGAGTATCAGAAGAAGAAACGTTGGTTGCATTGGAAAATGAAGTAGTAAGAGCATGGAAAGATGTAGTTGAAGACTATATGAAATCATCCAACATTTCAAAGGAGATCTGCATGCGTGTGTTGAATCTTGCACGCCTCTCAGACCGCTTTTATAAAGAGGAAGATGGTTACACATTCGCTGATGGAACAACCAAATGTTTCATTGCCTCTACCCTTGTCGACCCCGTACCAATA

mRNA sequence

TTCAAAACAAAAAGAAGATACACCAGCCATGGCCTCAAAAGATAATACCTCACTCGATGATGGGGTTGTTCCTCGTCGATTTGCAAACTTTCCACCAAGTGCATGGGGAGATTTCTTCATTTCCTATGATTCCAACACTATGGAAAATCAGCTCGAGGAGAAGATGGTCGAGAAACAGCTTCAAAAGCTTAAAGAAGAAGTGATGAACATGTTTGTGACAACAGACAAGTCTTCGGAAAAATTAAGGTTGATAGATTCAATTCAACGCTTAGGTTTGTCCCATCACTTTCAACTGGAAATTAATGAAACACTTCAACTTCTGCAAAGCTCTTCCGACGATGATGACGATATTTACAATATTGCCCTTAAGTTTCGATTGCTTAGACAAGAAGGTTATTCTATTTCATCTGAGATATTCAACAAGTTTATAAATGAAGAGGGAGAATTCAAAGAATCAATTGTGAAAGACAAAAGTGGAATATTAAGTTTGTACGAGGCGTCCCATTTAAGAATGAACGGAGAGTATACATTAGATAAAGCTTTGAGGTTCACAACTACTCATCTTCAAGAAATGGCCATGGATGAGAGCTCTCCTTTAGTAGATGAAGCCAAGCATGCTTTGAAGTGGCCAATCTATAAAACTGTGCCAAGGCTTATGACCAAGCACTACATTTCTGTCCATCAGAAGGATCCACCAAACATGATATTAAAAACCTATGAAGAGTTTGAGGAAGATATGAGCAAGGACATCATTAACTATGCCATTAATTATGCAAAAGAAGCGTTTAAAAGGCAGTGTAGAGTTTATTTTATGGAAGCTAAATGGTACCATGAAGGATATGTGCCAAGCATGGAGGAGTACATGAAAGTGTCCATTGTATCCACTTGTTACTATCTTTTTGCTCCAATTTCATTTCTTGGTATGGGCGTTGCTGCTTCTGAAGAGGCTTTCAAATGGGTTGAGAGTGATCCAATGATGCTCAAGGCAAGTGGAATAATTGGGAGGCTCATGAATGACATAACTTCACACAAGCAACATGGAGTATCAGAAGAAGAAACGTTGGTTGCATTGGAAAATGAAGTAGTAAGAGCATGGAAAGATGTAGTTGAAGACTATATGAAATCATCCAACATTTCAAAGGAGATCTGCATGCGTGTGTTGAATCTTGCACGCCTCTCAGACCGCTTTTATAAAGAGGAAGATGGTTACACATTCGCTGATGGAACAACCAAATGTTTCATTGCCTCTACCCTTGTCGACCCCGTACCAATA

Coding sequence (CDS)

ATGGCCTCAAAAGATAATACCTCACTCGATGATGGGGTTGTTCCTCGTCGATTTGCAAACTTTCCACCAAGTGCATGGGGAGATTTCTTCATTTCCTATGATTCCAACACTATGGAAAATCAGCTCGAGGAGAAGATGGTCGAGAAACAGCTTCAAAAGCTTAAAGAAGAAGTGATGAACATGTTTGTGACAACAGACAAGTCTTCGGAAAAATTAAGGTTGATAGATTCAATTCAACGCTTAGGTTTGTCCCATCACTTTCAACTGGAAATTAATGAAACACTTCAACTTCTGCAAAGCTCTTCCGACGATGATGACGATATTTACAATATTGCCCTTAAGTTTCGATTGCTTAGACAAGAAGGTTATTCTATTTCATCTGAGATATTCAACAAGTTTATAAATGAAGAGGGAGAATTCAAAGAATCAATTGTGAAAGACAAAAGTGGAATATTAAGTTTGTACGAGGCGTCCCATTTAAGAATGAACGGAGAGTATACATTAGATAAAGCTTTGAGGTTCACAACTACTCATCTTCAAGAAATGGCCATGGATGAGAGCTCTCCTTTAGTAGATGAAGCCAAGCATGCTTTGAAGTGGCCAATCTATAAAACTGTGCCAAGGCTTATGACCAAGCACTACATTTCTGTCCATCAGAAGGATCCACCAAACATGATATTAAAAACCTATGAAGAGTTTGAGGAAGATATGAGCAAGGACATCATTAACTATGCCATTAATTATGCAAAAGAAGCGTTTAAAAGGCAGTGTAGAGTTTATTTTATGGAAGCTAAATGGTACCATGAAGGATATGTGCCAAGCATGGAGGAGTACATGAAAGTGTCCATTGTATCCACTTGTTACTATCTTTTTGCTCCAATTTCATTTCTTGGTATGGGCGTTGCTGCTTCTGAAGAGGCTTTCAAATGGGTTGAGAGTGATCCAATGATGCTCAAGGCAAGTGGAATAATTGGGAGGCTCATGAATGACATAACTTCACACAAGCAACATGGAGTATCAGAAGAAGAAACGTTGGTTGCATTGGAAAATGAAGTAGTAAGAGCATGGAAAGATGTAGTTGAAGACTATATGAAATCATCCAACATTTCAAAGGAGATCTGCATGCGTGTGTTGAATCTTGCACGCCTCTCAGACCGCTTTTATAAAGAGGAAGATGGTTACACATTCGCTGATGGAACAACCAAATGTTTCATTGCCTCTACCCTTGTCGACCCCGTACCAATA

Protein sequence

MASKDNTSLDDGVVPRRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVTTDKSSEKLRLIDSIQRLGLSHHFQLEINETLQLLQSSSDDDDDIYNIALKFRLLRQEGYSISSEIFNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMDESSPLVDEAKHALKWPIYKTVPRLMTKHYISVHQKDPPNMILKTYEEFEEDMSKDIINYAINYAKEAFKRQCRVYFMEAKWYHEGYVPSMEEYMKVSIVSTCYYLFAPISFLGMGVAASEEAFKWVESDPMMLKASGIIGRLMNDITSHKQHGVSEEETLVALENEVVRAWKDVVEDYMKSSNISKEICMRVLNLARLSDRFYKEEDGYTFADGTTKCFIASTLVDPVPI
BLAST of Cp4.1LG08g07970 vs. Swiss-Prot
Match: TPSGD_VITVI ((-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.0e-48
Identity = 104/211 (49.29%), Postives = 144/211 (68.25%), Query Frame = 1

Query: 14  VPRRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVTTDK-SSEKL 73
           V RR ANF PS WGD F+SY S        +  +++ +Q+LKEEV  M +  D  S++KL
Sbjct: 20  VGRRCANFHPSIWGDHFLSYASEFTNT---DDHLKQHVQQLKEEVRKMLMAADDDSAQKL 79

Query: 74  RLIDSIQRLGLSHHFQLEINETLQLLQSSS--DDDDDIYNIALKFRLLRQEGYSISSEIF 133
            LID+IQRLG+++HF+ EI+E L+ +   S    ++D+Y  +L+FRLLRQ+GY +S ++F
Sbjct: 80  LLIDAIQRLGVAYHFESEIDEVLKHMFDGSVVSAEEDVYTASLRFRLLRQQGYHVSCDLF 139

Query: 134 NKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMDESSPL 193
           N F + EG FKES+  D  G+LSLYEA+H R++GE  LD+AL FTTTHLQ      S+PL
Sbjct: 140 NNFKDNEGNFKESLSSDVRGMLSLYEATHFRVHGEDILDEALAFTTTHLQSATKHSSNPL 199

Query: 194 VDEAKHALKWPIYKTVPRLMTKHYISVHQKD 222
            ++  HALK PI K +PRL  +HY SV+Q D
Sbjct: 200 AEQVVHALKQPIRKGLPRLEARHYFSVYQAD 227

BLAST of Cp4.1LG08g07970 vs. Swiss-Prot
Match: TPS3_RICCO (Probable terpene synthase 3 OS=Ricinus communis GN=TPS3 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 5.1e-48
Identity = 133/358 (37.15%), Postives = 206/358 (57.54%), Query Frame = 1

Query: 16  RRFANFPPSAWGDFFISYDSNTMENQLEEKMV---EKQLQKLKEEVMNMFVT-TDKSSEK 75
           RR A F PS WGD+FI       +N  + KMV   ++Q + L+EEV  M ++ T K S K
Sbjct: 15  RRTAQFHPSVWGDYFI-------KNAPDNKMVSIWKEQAKVLEEEVRRMIISETQKPSGK 74

Query: 76  LRLIDSIQRLGLSHHFQLEINETLQLLQSSSDDDDDIYNIALKFRLLRQEGYSISSEIFN 135
           L+LID IQRLG+++HF+ EI E ++ + S+ DDD+D+Y+IAL+FRLLRQ+GY++SS++F+
Sbjct: 75  LKLIDVIQRLGIAYHFEEEIGEVIEQVYSNYDDDEDLYDIALRFRLLRQQGYNVSSDVFD 134

Query: 136 KFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMDESSPLV 195
           KF + +G+FK+ +V D  G+LSL+EAS++ + GE  LD+AL FT THL  MA   SSPL 
Sbjct: 135 KFKDCKGDFKKHLVNDVQGLLSLHEASYMSVQGEKILDEALEFTKTHL--MATQLSSPLP 194

Query: 196 DEAKHALKWPIYKTVPRLMTKHYISVHQKDPPNMILKTYEEFEEDMSKDIINYAINYAKE 255
           D+  HAL+WP+ + +PR     Y S++Q+D          E  E + K +     N  ++
Sbjct: 195 DQVSHALRWPVRRGLPRKEAWQYFSIYQQD---------REHIEPLLK-LAKLDFNIVQK 254

Query: 256 AFKRQCRVYFMEAKWYHEGYVPSMEEYMKVSIVSTCYYLFAPISFLGMGVAASEEAFKWV 315
              +   +     +W+ +    +   + +  ++          SF  +GV    +   +V
Sbjct: 255 LHHKDMSII---TRWWIDLDFTTKLSFARDRVIE--------CSFWALGVFYEPQ---FV 314

Query: 316 ESDPMMLKASGIIGRLMNDITSHKQHGVSEEETLVALENEVVRAW----KDVVEDYMK 366
            +  ++ KA  I+  +M+DI  +  HG  EE   + L  EVV  W    KD + DYMK
Sbjct: 315 FARQVLSKAVAILS-VMDDI--YDVHGTIEE---LELFTEVVERWDISMKDQLPDYMK 333

BLAST of Cp4.1LG08g07970 vs. Swiss-Prot
Match: TPS2_RICCO (Probable terpene synthase 2 OS=Ricinus communis GN=TPS2 PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.3e-43
Identity = 95/237 (40.08%), Postives = 158/237 (66.67%), Query Frame = 1

Query: 16  RRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVTTDKSSEKLRLI 75
           R  A+F P+ WGD F+S  + + +  +E+ M E+Q+++LKEEV  M  + D+ S+ L LI
Sbjct: 17  RHLASFHPNIWGDRFLSCAAESTD--IEDDM-EQQVERLKEEVKKMIASADEPSQILNLI 76

Query: 76  DSIQRLGLSHHFQLEINETLQL---LQSSSDDDDDIYNIALKFRLLRQEGYSISSEIFNK 135
           D +QRLG+S+HF+ EI E LQ    + S SD DDD++++AL+FRLLR++G ++S ++FNK
Sbjct: 77  DLLQRLGVSYHFEKEIEEALQQVLNMNSDSDKDDDLHSVALRFRLLREQGLNVSCDVFNK 136

Query: 136 FINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMDESSPLVD 195
           F +  G F +++  D  G+LSLYEA+H R++GE  LD AL FTTT+L+ +  + S PL  
Sbjct: 137 FRDRNGHFIQTLKTDLQGMLSLYEAAHFRVHGEGILDDALAFTTTYLESIVPNLSPPLAA 196

Query: 196 EAKHALKWPIYKTVPRLMTKHYISVHQKDPP-NMILKTYEEFEEDMSKDIINYAINY 249
           +    L+ P+ K++ R+  +H+IS++Q+D   N +L T+ + + ++ + +    + Y
Sbjct: 197 QISRTLRQPLRKSLARVEARHFISIYQEDTSHNEVLLTFAKLDFNLLQKLHQKELKY 250

BLAST of Cp4.1LG08g07970 vs. Swiss-Prot
Match: AFSY1_CUCME (Alpha-farnesene synthase OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.9e-43
Identity = 106/243 (43.62%), Postives = 149/243 (61.32%), Query Frame = 1

Query: 14  VPRRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMF--VTTDKSSEK 73
           V RR A F  S WGD+F+SY S + E     K++EKQ ++LKEE+       T D+  EK
Sbjct: 14  VIRRSAQFQASVWGDYFLSYHSLSPEKG--NKVMEKQTEELKEEIKRELNSTTKDEEPEK 73

Query: 74  LRLIDSIQRLGLSHHFQLEINETLQLLQ-----SSSDDDDDIYNIALKFRLLRQEGYSIS 133
           LRLIDSIQRLG+ +HF+ EIN+ L+ L      S ++ DD  YN+ L+FRLLRQ+GY+IS
Sbjct: 74  LRLIDSIQRLGVCYHFEYEINKILEQLHHITITSKNNGDDHPYNMTLRFRLLRQQGYNIS 133

Query: 134 SEIFNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMDE 193
           S+ F +F    G+++ S  K+   +LSLYEAS LRM GE  LD+A RF T  L+ +  D 
Sbjct: 134 SKSFERF---RGKWESSYDKNVEELLSLYEASQLRMRGEEALDEAFRFATAQLEAIVQDP 193

Query: 194 SS--PLVDEAKHALKWPIYKTVPRLMTKHYISVHQKDP------PNMILKTYEEFEEDMS 242
           ++   +V E   ALKWP+YK +PRL   HYI ++ + P      PN     + + ++   
Sbjct: 194 TTDPTVVGEVCQALKWPMYKNLPRLQASHYIGLYSEKPWRNESLPNFAKMDFSKLQKLHQ 251

BLAST of Cp4.1LG08g07970 vs. Swiss-Prot
Match: STS1_THAGA (Sesquiterpene synthase 1 OS=Thapsia garganica GN=STS1 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 6.4e-43
Identity = 98/215 (45.58%), Postives = 146/215 (67.91%), Query Frame = 1

Query: 14  VPRRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVTTDKSSEKLR 73
           V R  A F PS WGD FI+  ++ ++    ++  E++LQ LK+EV  M    D S + L 
Sbjct: 15  VGRNSAGFHPSIWGDKFITSSNSAVQKTDVDRKEEEKLQLLKQEVKKMLTAGDTSQQDLI 74

Query: 74  -LIDSIQRLGLSHHFQLEINETLQLLQSS------SDDDDDIYNIALKFRLLRQEGYSIS 133
            LID IQRLGLS+HF+ EI+  LQ ++ S        + D+++++AL FRLLRQEG++IS
Sbjct: 75  CLIDDIQRLGLSYHFEAEIDTLLQHVKHSYVEHYGKKNHDNLHDVALSFRLLRQEGHNIS 134

Query: 134 SEIFNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQE-MAMD 193
           S++F+KF + +G+FKE +V+D  G+LSL+EA+HLR++GE  L+ AL FTTTHL   +  +
Sbjct: 135 SDVFSKFQDSDGKFKEELVEDVRGMLSLFEAAHLRVHGENILEDALEFTTTHLNSYLNSN 194

Query: 194 ESSPLVDEAKHALKWPIYKTVPRLMTKHYISVHQK 221
            SS L D  + ALK+P+ K+  R++ +HYISV+ K
Sbjct: 195 PSSSLADLVRRALKYPLRKSFNRMVARHYISVYHK 229

BLAST of Cp4.1LG08g07970 vs. TrEMBL
Match: A0A0A0L1X6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G039850 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.7e-72
Identity = 141/209 (67.46%), Postives = 164/209 (78.47%), Query Frame = 1

Query: 226 ILKTYEEFEEDMSKD-IINYAINYAKEAFKRQCRVYFMEAKWYHEGYVPSMEEYMKVSIV 285
           ILK YEE E+D++KD II YAI+YAKE  KRQCRVYF EAKW+HEGYVP+ EEYMKV+IV
Sbjct: 354 ILKVYEEIEKDINKDNIIPYAIHYAKEGMKRQCRVYFEEAKWFHEGYVPTFEEYMKVAIV 413

Query: 286 STCYYLFAPISFLGMGVAASEEAFKWVESDPMMLKASGIIGRLMNDITSH---------- 345
           STCYYLF PISF+GMG+AAS+EAF+WVESDPM+LKASGI+GRLMNDITSH          
Sbjct: 414 STCYYLFVPISFVGMGIAASQEAFEWVESDPMLLKASGIVGRLMNDITSHKYEQKRGHMD 473

Query: 346 -------KQHGVSEEETLVALENEVVRAWKDVVEDY-MKSSNISKEICMRVLNLARLSDR 405
                  KQH VSEE+ +V L  E+ +AWKDV+EDY MKS+ +S  I MRVLNL RLSD 
Sbjct: 474 SAVECYMKQHEVSEEDAIVELGKEITKAWKDVIEDYIMKSTKLSNAILMRVLNLTRLSDL 533

Query: 406 FYKEEDGYTFADGTTKCFIASTLVDPVPI 416
           FYK+EDGYTF  G TK FI S L+DP+PI
Sbjct: 534 FYKKEDGYTFVHGDTKHFITSMLIDPIPI 562

BLAST of Cp4.1LG08g07970 vs. TrEMBL
Match: A0A0B4VFP0_CUCME (Sesquiterpene synthase Tps3 OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 5.3e-68
Identity = 132/209 (63.16%), Postives = 161/209 (77.03%), Query Frame = 1

Query: 226 ILKTYEEFEEDMSKDIIN-YAINYAKEAFKRQCRVYFMEAKWYHEGYVPSMEEYMKVSIV 285
           ILK YEE E+D+SKD IN YAI++AKE  KRQCR YF EAKW+HEGY+P+ EEYMKV+IV
Sbjct: 314 ILKVYEEIEKDISKDNINPYAIHFAKEGMKRQCRAYFAEAKWFHEGYMPTFEEYMKVAIV 373

Query: 286 STCYYLFAPISFLGMGVAASEEAFKWVESDPMMLKASGIIGRLMNDITSH---------- 345
           STC+Y++ PISF+GMGVAAS+E F+WVESDPM+LKASGIIGRLMNDITSH          
Sbjct: 374 STCFYVYVPISFVGMGVAASQEVFEWVESDPMLLKASGIIGRLMNDITSHKFEQERGHID 433

Query: 346 -------KQHGVSEEETLVALENEVVRAWKDVVEDY-MKSSNISKEICMRVLNLARLSDR 405
                  KQH VSEE+ ++ L  E+ +AWKD++EDY MKS+ +S  I MRVLN  RLS+ 
Sbjct: 434 SAVECYMKQHEVSEEDAIIELAKEITKAWKDMIEDYIMKSTKLSTAILMRVLNHGRLSEF 493

Query: 406 FYKEEDGYTFADGTTKCFIASTLVDPVPI 416
           FYK+EDGYTF  G TK FI + L+DP+PI
Sbjct: 494 FYKKEDGYTFVHGDTKHFITAMLIDPIPI 522

BLAST of Cp4.1LG08g07970 vs. TrEMBL
Match: A0A0A0L4K8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G041370 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 3.8e-66
Identity = 135/236 (57.20%), Postives = 169/236 (71.61%), Query Frame = 1

Query: 199 KWPIYKT--VPRLMTKHYISVHQKDPPNMILKTYEEFEEDMSKDIINYAINYAKEAFKRQ 258
           +W I +T  +P+ M   Y          +I K YEE +  ++KD I YAI+Y KEA KRQ
Sbjct: 164 RWDINETSKLPKYMKYFY---------EVIFKYYEEIDNSINKDNIQYAIHYVKEAMKRQ 223

Query: 259 CRVYFMEAKWYHEGYVPSMEEYMKVSIVSTCYYLFAPISFLGMGVAASEEAFKWVESDPM 318
           CRVYF EAKW++EGYVP+MEEYMKV+IVSTCY+LF  ISF+GMGVAAS+EAF+W+ESDPM
Sbjct: 224 CRVYFEEAKWFYEGYVPTMEEYMKVAIVSTCYHLFVQISFVGMGVAASQEAFQWIESDPM 283

Query: 319 MLKASGIIGRLMNDITSH-----------------KQHGVSEEETLVALENEVVRAWKDV 378
           +LKASGIIGRL+NDITSH                 KQ+ VSEE  ++ L+ E+ +AWKD 
Sbjct: 284 LLKASGIIGRLINDITSHQFEQQRGHIASAVECYMKQYEVSEEVAVIELQKEIEKAWKDG 343

Query: 379 VEDY-MKSSNISKEICMRVLNLARLSDRFYKEEDGYTFADGTTKCFIASTLVDPVP 415
           +EDY MKS+  S  I MRVLNLARLSD +YK+EDGYTF+ G TK F+   L+DP+P
Sbjct: 344 IEDYIMKSTKFSNAILMRVLNLARLSDFYYKKEDGYTFSHGETKDFVTYMLIDPLP 390

BLAST of Cp4.1LG08g07970 vs. TrEMBL
Match: A5BBY5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021510 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 6.4e-66
Identity = 168/472 (35.59%), Postives = 249/472 (52.75%), Query Frame = 1

Query: 14  VPRRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVTTDKSS-EKL 73
           V RR AN+ PS WGD F+SY S        +  +++ +Q+LKEEV  M +  D  S +KL
Sbjct: 20  VGRRCANYHPSIWGDHFLSYASEFTNT---DDHLKQHVQQLKEEVRKMLMAADDDSXQKL 79

Query: 74  RLIDSIQRLGLSHHFQLEINETLQLLQSSS--DDDDDIYNIALKFRLLRQEGYSIS-SEI 133
            LID+IQRLG+++HF+ EI+E L+ +   S    ++D+Y  +L+FRLLRQ+GY +S +++
Sbjct: 80  LLIDAIQRLGVAYHFESEIDEXLKHMFDXSVXSAEEDVYTASLRFRLLRQQGYHVSCADL 139

Query: 134 FNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTL-----DKALRFTTTHLQEMAM 193
           FN F + EG FKES+  D  G+LSLYEA+HLR   ++ L      K L   +    ++  
Sbjct: 140 FNNFKDNEGNFKESLSSDVRGMLSLYEATHLRPKLDFNLLQKLHQKELSDISAWWXDLDF 199

Query: 194 DESSPLV-DEAKHALKW-------PIYKTVPRLMTKHY---------------------- 253
               P   D       W       P +    R++TK                        
Sbjct: 200 AHKLPFARDRVVECYFWILGVYFXPQFFLARRILTKVITMTSTIDDIYDVYGTLEELELF 259

Query: 254 --------ISVHQKDPPNM------ILKTYEEFEEDMSKDIINYAINYAKEAFKRQCRVY 313
                   ISV  + P  M      +L  Y E EE+M+K+  +Y   YAKEA K+Q R Y
Sbjct: 260 TEAVERWDISVIDQLPEYMRVCYRALLDVYSEIEEEMAKEGRSYRFYYAKEAMKKQVRAY 319

Query: 314 FMEAKWYHEGYVPSMEEYMKVSIVSTCYYLFAPISFLGMGVAASEEAFKWVESDPMMLKA 373
           + EA+W     + +MEEYM V++ ++ Y + A  SF+ MG   ++E F WV S+P +++A
Sbjct: 320 YEEAQWLQAQQIXTMEEYMPVALATSGYLMLATTSFIAMGDVVTKETFDWVFSEPKIVRA 379

Query: 374 SGIIGRLMNDITSH-----------------KQHGVSEEETLVALENEVVRAWKDVVEDY 416
           S  + RLM+D+ SH                 KQHG SE+ET    + +V  AWKD+ ++ 
Sbjct: 380 SATVSRLMDDMVSHKFEQKRGHVASAVECYMKQHGASEQETRDEFKKQVRDAWKDINQEC 439

BLAST of Cp4.1LG08g07970 vs. TrEMBL
Match: A0A0A0L782_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040850 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.3e-63
Identity = 147/306 (48.04%), Postives = 199/306 (65.03%), Query Frame = 1

Query: 1   MASKDNTSLDDGVVPRRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMN 60
           M S  N+S +D  VP R   F  S+ G +F+S+   T+E   E+KM+ K+LQ+LKEEV +
Sbjct: 15  MTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLE---EDKMIRKKLQELKEEVRS 74

Query: 61  MFVTTDKSSEKLRLIDSIQRLGLSHHFQLEINETLQLLQSSSDDDD---DIYNIALKFRL 120
           MFVTT+K S+KL LIDSIQRLGLS+HF  EINE L L+++  + DD   D+Y  AL+FRL
Sbjct: 75  MFVTTNKFSKKLSLIDSIQRLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYITALRFRL 134

Query: 121 LRQEGYSISSEIFNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTT 180
           LRQ+G+ +S EIFNKF NE GE KESI KD++ +LSLYEASHLRM GE  LD+AL FTTT
Sbjct: 135 LRQQGFFVSCEIFNKFTNERGELKESIRKDENSLLSLYEASHLRMKGENILDEALAFTTT 194

Query: 181 HLQEMAMDESSPLVDEAKHALKWPIYKTVPRLMTKHYISVHQKDP-PNMILKTYEEFEED 240
           HL+ MAMD +SP  +EAK+ALKWPIYK V R M +HYIS++  +P  N  L T+ + + +
Sbjct: 195 HLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNNALLTFAKLDYN 254

Query: 241 MSKDIINYAINYAKEAFKRQCRVYFMEAKWYH-----EGYVPSMEEYMKVSIVSTCYYLF 298
             + +    ++     +K Q    FME   +      E Y+  +E Y K +  +      
Sbjct: 255 SLQKLYQKELSELSRWWKNQ---KFMEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFL 314

BLAST of Cp4.1LG08g07970 vs. TAIR10
Match: AT5G23960.1 (AT5G23960.1 terpene synthase 21)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-27
Identity = 72/216 (33.33%), Postives = 126/216 (58.33%), Query Frame = 1

Query: 14  VPRRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVTTDKSS-EKL 73
           V R  A+FP + W D   S+     ++ L  +  +++   LKE V   F+++  +  E +
Sbjct: 5   VNRPLADFPANIWEDPLTSFS----KSDLGTETFKEKHSTLKEAVKEAFMSSKANPIENI 64

Query: 74  RLIDSIQRLGLSHHFQLEINETLQ-------LLQSSSDDDDDIYNIALKFRLLRQEGYSI 133
           + ID++ RLG+S+HF+ +I E L          Q    +  D+Y + + F++ RQ G+ +
Sbjct: 65  KFIDALCRLGVSYHFEKDIVEQLDKSFDCLDFPQMVRQEGCDLYTVGIIFQVFRQFGFKL 124

Query: 134 SSEIFNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMD 193
           S+++F KF +E G+FK  +V D  G+LSLYEA+    +GE  +D+AL F+ +HL+E++  
Sbjct: 125 SADVFEKFKDENGKFKGHLVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLEEISSR 184

Query: 194 ESSPLVDEAKHALKWPIYKTVPRLMTKHYISVHQKD 222
            S  L    K+ALK P +K + R+ T+ YIS ++++
Sbjct: 185 SSPHLAIRIKNALKHPYHKGISRIETRQYISYYEEE 216

BLAST of Cp4.1LG08g07970 vs. TAIR10
Match: AT1G48800.1 (AT1G48800.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 119.8 bits (299), Expect = 4.0e-27
Identity = 88/263 (33.46%), Postives = 143/263 (54.37%), Query Frame = 1

Query: 16  RRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVTTD--KSSEK-L 75
           R+F    PS WG+ F+    +  E       +E++++ LK +V +MF++    KS++K L
Sbjct: 63  RKFEKLGPSEWGNQFLFAHVDLSEMDA----LEREIEALKPKVRDMFMSFKGMKSNKKNL 122

Query: 76  RLIDSIQRLGLSHHFQLEINETL----QLLQSSSDDDDDIYNIALKFRLLRQEGYSISSE 135
            LI  +  LGL+HHF+ EI E++    Q +    D ++D+Y +++ F + R  G++ISS+
Sbjct: 123 FLIYLLVSLGLAHHFEDEIEESVKGCSQEMVEMMDGENDLYTVSIIFWVFRTYGHNISSD 182

Query: 136 IFNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMDESS 195
           IFN+F    G+FKE +  D  GILSLYEA+H+    +Y LD+AL FT ++L+ +A + + 
Sbjct: 183 IFNRFKGHNGKFKECLATDAKGILSLYEAAHMGTTTDYILDEALSFTLSYLESLAANGTC 242

Query: 196 P--LVDEAKHALKWPIYKTVPRLMTKHYISVHQKDPPNMILKTYEEFEEDMSKDIINYAI 255
              LV   ++AL     K V  L+ K YI            + YE+ EED  K I+ +++
Sbjct: 243 KPNLVRRIRNALGLLQNKNVEILVAKEYI------------RFYEQ-EEDCDKTILEFSM 302

Query: 256 NYAKEAFKRQCRVYFMEAKWYHE 270
              K       +   +  KWY E
Sbjct: 303 LNLKFLQLHYLQELKLLTKWYKE 308

BLAST of Cp4.1LG08g07970 vs. TAIR10
Match: AT4G20210.1 (AT4G20210.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 117.5 bits (293), Expect = 2.0e-26
Identity = 85/285 (29.82%), Postives = 145/285 (50.88%), Query Frame = 1

Query: 16  RRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVTTDKSSEKLRLI 75
           R+F  FPPS W + F S   +  E     K ++K +  +K+E+M+     + + +K+ ++
Sbjct: 58  RKFQKFPPSEWTNRFDSVSVDASEMDALRKEIDKIIPNVKKELMSS-QGIESTKKKILMV 117

Query: 76  DSIQRLGLSHHFQLEINETL----QLLQSSSDDDDDIYNIALKFRLLRQEGYSISSEIFN 135
             +  LGL++HF+ EI E L    + ++     +D++Y I++ F +LR  G+ +SS+IF 
Sbjct: 118 YLLVSLGLAYHFEDEIEECLKEGFETIEEMMAGEDNLYTISIIFLVLRTYGHHMSSDIFQ 177

Query: 136 KFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMDESSP-- 195
           KF   +G FK  I  D  G+L+LYEA+ LR   EY +++AL FT+++L+ +A D   P  
Sbjct: 178 KFKGNDGNFKGCISGDAKGLLALYEAAQLRTTTEYIMEEALSFTSSNLELLAADGRCPPH 237

Query: 196 LVDEAKHALKWPIYKTVPRLMTKHYISVHQKDPPNMILKTYEEFEEDMSKDIINYAINYA 255
           L    ++AL    +K +  L+   YIS ++++                 KD     + +A
Sbjct: 238 LSKHIRNALGLSQHKQMEVLVAVEYISFYEQE-----------------KDHDKILLKFA 297

Query: 256 KEAFKRQCRVYFME----AKWYHE-GYVPSMEEYMKVSIVSTCYY 290
           K  FK     Y  E     KWY E  +  ++  Y K  IV   ++
Sbjct: 298 KLNFKLMQLHYLEELKVVTKWYKEHDFASNLPPYFKYVIVENHFF 324

BLAST of Cp4.1LG08g07970 vs. TAIR10
Match: AT4G20230.1 (AT4G20230.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 117.1 bits (292), Expect = 2.6e-26
Identity = 102/363 (28.10%), Postives = 168/363 (46.28%), Query Frame = 1

Query: 1   MASKDNTSLDDGVVPRRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMN 60
           + + +  + DD    R+F   P S W  +F S   +  E       +++++ +LK +V N
Sbjct: 49  LKATNTLTFDDKERTRKFKKLPLSEWTHYFHSIPLDISEMDA----LKEEIDELKPKVKN 108

Query: 61  MFVTT---DKSSEKLRLIDSIQRLGLSHHFQLEINETL----QLLQSSSDDDDDIYNIAL 120
            F+++   D +  K+ +I     LGL++HF+ EI ETL    + ++     ++D+Y +++
Sbjct: 109 TFMSSQGSDSTKTKILMIYLFVSLGLAYHFEEEIYETLKEGFENIEKIMAGEEDLYTVSI 168

Query: 121 KFRLLRQEGYSISSEIFNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALR 180
            F + R+ G+ ISS++F +F    G FKES++ D  G+LSLYEA+HL    +Y LD+AL 
Sbjct: 169 IFWVFRRYGHYISSDVFQRFKGSNGSFKESLIGDAKGMLSLYEAAHLATTKDYILDEALI 228

Query: 181 FTTTHLQEMAMDESSP--LVDEAKHALK----WPIYKTVPRLMTKHYISVHQKDPPNMIL 240
           FT++HL+ +    + P  L+   ++AL     W     VP      Y    +KD   M+L
Sbjct: 229 FTSSHLETLVATGTCPPHLLARIRNALSICQHWNFEVLVPLDFIPFY--EQEKDHDEMLL 288

Query: 241 KTYEEFEEDMSKDIINYAINYAKEAFKRQCRVYFME----AKWYHEGYVPS-MEEYMKVS 300
           K                   +AK +FK    +Y  +     KWY +   PS    Y K  
Sbjct: 289 K-------------------FAKLSFKYLKLIYLQDLKILTKWYKKLDFPSKFPPYFKDR 348

Query: 301 IVSTCYYLFAPISFLGMGVAASEEAFKWVESDPMMLKASGIIGRLMNDITSHKQHGVSEE 346
            V   Y+   P+             F+   S   ML   G I   + D T  +   +SE 
Sbjct: 349 CVEN-YFFVLPV------------FFEPQLSSARMLLTKGFILLGIQDDTFDRYASISEA 373

BLAST of Cp4.1LG08g07970 vs. TAIR10
Match: AT5G44630.1 (AT5G44630.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 115.2 bits (287), Expect = 9.9e-26
Identity = 75/224 (33.48%), Postives = 127/224 (56.70%), Query Frame = 1

Query: 18  FANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVT--TDKSSEKLRL- 77
           F   P S WGD F+ +     +  + E+ +E    K++E   N+FV+  TDK + K  + 
Sbjct: 15  FTKLPSSQWGDQFLKFSIADSDFDVLEREIEVLKPKVRE---NIFVSSSTDKDAMKKTIL 74

Query: 78  -IDSIQRLGLSHHFQLEINETL----QLLQSSSDDDDDIYNIALKFRLLRQEGYSISSEI 137
            I  +  LGLS+HF+ EI E+L    + ++    D++ ++ I+  FR+ R  GY +SS++
Sbjct: 75  SIHFLDSLGLSYHFEKEIEESLKHAFEKIEDLIADENKLHTISTIFRVFRTYGYYMSSDV 134

Query: 138 FNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMDE--S 197
           F  F  ++G+FKES+++D  G+LS YEA H     ++ LD+AL FT  HL+ +A     S
Sbjct: 135 FKIFKGDDGKFKESLIEDVKGMLSFYEAVHFGTTTDHILDEALSFTLNHLESLATGRRAS 194

Query: 198 SPLVDE-AKHALKWPIYKTVPRLMTKHYISV--HQKDPPNMILK 229
            P + +  ++AL  P ++ +  L+ + YIS   H++D    +LK
Sbjct: 195 PPHISKLIQNALHIPQHRNIQALVAREYISFYEHEEDHDETLLK 235

BLAST of Cp4.1LG08g07970 vs. NCBI nr
Match: gi|659096594|ref|XP_008449179.1| (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 293.9 bits (751), Expect = 4.4e-76
Identity = 145/209 (69.38%), Postives = 170/209 (81.34%), Query Frame = 1

Query: 225 MILKTYEEFEEDMSKD--IINYAINYAKEAFKRQCRVYFMEAKWYHEGYVPSMEEYMKVS 284
           +ILK YEE E+D++KD   I YAI+YAKE  KRQCR YF EAKW+HEGYVP+ EEYMKV+
Sbjct: 183 VILKLYEEIEKDINKDNVTIPYAIHYAKEGMKRQCRAYFAEAKWFHEGYVPTFEEYMKVA 242

Query: 285 IVSTCYYLFAPISFLGMGVAASEEAFKWVESDPMMLKASGIIGRLMNDITSH-------- 344
            VSTCYYLF PISF+GMGVAAS+EAF+WVESDPM+LKASGIIGRLMND+TSH        
Sbjct: 243 TVSTCYYLFVPISFVGMGVAASKEAFEWVESDPMLLKASGIIGRLMNDVTSHTFEQERGD 302

Query: 345 ---------KQHGVSEEETLVALENEVVRAWKDVVEDYMKSSNISKEICMRVLNLARLSD 404
                    KQHG+SEEETLV LENEV++AW+D+ EDY+K++NIS EI +RVLNLARLSD
Sbjct: 303 VGSAVECHMKQHGLSEEETLVELENEVIKAWRDITEDYIKTTNISNEILLRVLNLARLSD 362

Query: 405 RFYKEEDGYTFADGTTKCFIASTLVDPVP 415
            FYKEEDGYTF D TTK FIAS +V+P+P
Sbjct: 363 LFYKEEDGYTFVDKTTKHFIASIIVEPLP 391

BLAST of Cp4.1LG08g07970 vs. NCBI nr
Match: gi|778675684|ref|XP_011650453.1| (PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus])

HSP 1 Score: 280.8 bits (717), Expect = 3.9e-72
Identity = 141/209 (67.46%), Postives = 164/209 (78.47%), Query Frame = 1

Query: 226 ILKTYEEFEEDMSKD-IINYAINYAKEAFKRQCRVYFMEAKWYHEGYVPSMEEYMKVSIV 285
           ILK YEE E+D++KD II YAI+YAKE  KRQCRVYF EAKW+HEGYVP+ EEYMKV+IV
Sbjct: 354 ILKVYEEIEKDINKDNIIPYAIHYAKEGMKRQCRVYFEEAKWFHEGYVPTFEEYMKVAIV 413

Query: 286 STCYYLFAPISFLGMGVAASEEAFKWVESDPMMLKASGIIGRLMNDITSH---------- 345
           STCYYLF PISF+GMG+AAS+EAF+WVESDPM+LKASGI+GRLMNDITSH          
Sbjct: 414 STCYYLFVPISFVGMGIAASQEAFEWVESDPMLLKASGIVGRLMNDITSHKYEQKRGHMD 473

Query: 346 -------KQHGVSEEETLVALENEVVRAWKDVVEDY-MKSSNISKEICMRVLNLARLSDR 405
                  KQH VSEE+ +V L  E+ +AWKDV+EDY MKS+ +S  I MRVLNL RLSD 
Sbjct: 474 SAVECYMKQHEVSEEDAIVELGKEITKAWKDVIEDYIMKSTKLSNAILMRVLNLTRLSDL 533

Query: 406 FYKEEDGYTFADGTTKCFIASTLVDPVPI 416
           FYK+EDGYTF  G TK FI S L+DP+PI
Sbjct: 534 FYKKEDGYTFVHGDTKHFITSMLIDPIPI 562

BLAST of Cp4.1LG08g07970 vs. NCBI nr
Match: gi|745672490|gb|AJD19683.1| (sesquiterpene synthase Tps3 [Cucumis melo])

HSP 1 Score: 266.5 bits (680), Expect = 7.6e-68
Identity = 132/209 (63.16%), Postives = 161/209 (77.03%), Query Frame = 1

Query: 226 ILKTYEEFEEDMSKDIIN-YAINYAKEAFKRQCRVYFMEAKWYHEGYVPSMEEYMKVSIV 285
           ILK YEE E+D+SKD IN YAI++AKE  KRQCR YF EAKW+HEGY+P+ EEYMKV+IV
Sbjct: 314 ILKVYEEIEKDISKDNINPYAIHFAKEGMKRQCRAYFAEAKWFHEGYMPTFEEYMKVAIV 373

Query: 286 STCYYLFAPISFLGMGVAASEEAFKWVESDPMMLKASGIIGRLMNDITSH---------- 345
           STC+Y++ PISF+GMGVAAS+E F+WVESDPM+LKASGIIGRLMNDITSH          
Sbjct: 374 STCFYVYVPISFVGMGVAASQEVFEWVESDPMLLKASGIIGRLMNDITSHKFEQERGHID 433

Query: 346 -------KQHGVSEEETLVALENEVVRAWKDVVEDY-MKSSNISKEICMRVLNLARLSDR 405
                  KQH VSEE+ ++ L  E+ +AWKD++EDY MKS+ +S  I MRVLN  RLS+ 
Sbjct: 434 SAVECYMKQHEVSEEDAIIELAKEITKAWKDMIEDYIMKSTKLSTAILMRVLNHGRLSEF 493

Query: 406 FYKEEDGYTFADGTTKCFIASTLVDPVPI 416
           FYK+EDGYTF  G TK FI + L+DP+PI
Sbjct: 494 FYKKEDGYTFVHGDTKHFITAMLIDPIPI 522

BLAST of Cp4.1LG08g07970 vs. NCBI nr
Match: gi|659096596|ref|XP_008449181.1| (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 265.0 bits (676), Expect = 2.2e-67
Identity = 132/209 (63.16%), Postives = 161/209 (77.03%), Query Frame = 1

Query: 226 ILKTYEEFEEDMSKDIIN-YAINYAKEAFKRQCRVYFMEAKWYHEGYVPSMEEYMKVSIV 285
           ILK YEE E+D+SKD IN YAI++AKE  KRQCR YF EAKW+HEGY+P+ EEYMKV+IV
Sbjct: 355 ILKVYEEIEKDISKDNINPYAIHFAKEGMKRQCRAYFAEAKWFHEGYMPTFEEYMKVAIV 414

Query: 286 STCYYLFAPISFLGMGVAASEEAFKWVESDPMMLKASGIIGRLMNDITSH---------- 345
           STC+Y++ PISF+GMGVAAS+E F+WVESDPM+LKASGIIGRLMNDITSH          
Sbjct: 415 STCFYVYVPISFVGMGVAASQEVFEWVESDPMLLKASGIIGRLMNDITSHKFEQERGHID 474

Query: 346 -------KQHGVSEEETLVALENEVVRAWKDVVEDY-MKSSNISKEICMRVLNLARLSDR 405
                  KQH VSEE+ +V L  E+ +AWKD++EDY MKS+ +S  I +RVLN  RLS+ 
Sbjct: 475 SAVECYMKQHEVSEEDAIVELGKEITKAWKDMIEDYIMKSTKLSTAIVIRVLNHGRLSEF 534

Query: 406 FYKEEDGYTFADGTTKCFIASTLVDPVPI 416
           FYK+EDGYTF  G TK FI + L+DP+PI
Sbjct: 535 FYKKEDGYTFVHGDTKHFITAMLIDPIPI 563

BLAST of Cp4.1LG08g07970 vs. NCBI nr
Match: gi|970040273|ref|XP_015081514.1| (PREDICTED: probable terpene synthase 3 [Solanum pennellii])

HSP 1 Score: 263.5 bits (672), Expect = 6.4e-67
Identity = 154/427 (36.07%), Postives = 245/427 (57.38%), Query Frame = 1

Query: 14  VPRRFANFPPSAWGDFFISYDSNTMENQLEEKMVEKQLQKLKEEVMNMFVTT-DKSSEKL 73
           + RR  N  PS WGD F++Y +    N+ EEK    + + LKE V  M V +  KS +KL
Sbjct: 10  ITRRSDNHHPSVWGDHFLAYANLLGANEWEEK----EHKDLKEGVRKMLVISPSKSLQKL 69

Query: 74  RLIDSIQRLGLSHHFQLEINETLQLLQSSSDD------DDDIYNIALKFRLLRQEGYSIS 133
            LI++IQ LG+S+HF+ EI E+L  + +  ++      + D++ +AL FRLLRQ+GY +S
Sbjct: 70  ELINTIQLLGVSYHFEHEIEESLSEICNGYEEWIGEFGESDLHVVALSFRLLRQQGYYVS 129

Query: 134 SEIFNKFINEEGEFKESIVKDKSGILSLYEASHLRMNGEYTLDKALRFTTTHLQEMAMDE 193
           S++F KF +++G++K ++V D  G+LSLYEA+   ++ E  LD+A+ F+TTHL  +    
Sbjct: 130 SDVFRKFTDDQGDYKTALVNDTHGLLSLYEAAQFIVHDEEILDEAINFSTTHLNLLLPKL 189

Query: 194 SSPLVDEAKHALKWPIYKTVPRLMTKHYISVHQKDPPNMILKTYEEFEEDMSKDIINYAI 253
           S+ L  +   ALK+P++          Y  ++       +L  Y E E+++ K+  ++ +
Sbjct: 190 SNSLSMQVSCALKYPMWNIDATENLASYTKLYY----TALLHFYNEVEKELEKENKSFRV 249

Query: 254 NYAKEAFKRQCRVYFMEAKWYHEGYVPSM-EEYMKVSIVSTCYYLFAPISFLGMGVAASE 313
           N+A    K+  R YF EAKWYH   VP M EEYMK  I ++     A  S+LGM   A++
Sbjct: 250 NFAISEMKKLVRAYFQEAKWYHGNTVPKMEEEYMKNGIQTSTIPTLATASWLGMDDEATK 309

Query: 314 EAFKWVESDPMMLKASGIIGRLMNDITSHK-----------------QHGVSEEETLVAL 373
           EAF+W+ ++P +L AS  I RL+NDI SH+                 +H  ++EE+ + +
Sbjct: 310 EAFEWITTEPPILVASSNIARLLNDIVSHEREIERGDVASSIDCYMNEHDATKEESYMEI 369

Query: 374 ENEVVRAWKDVVEDYMKSSNISKEICMRVLNLARLSDRFYKEEDGYTFADGTTKCFIAST 416
              V   WKD+    +K + + + + M +LNL R S+ +YK+ D YTF+    K  I+  
Sbjct: 370 RKIVENNWKDLNRGCLKPTTVPRVLLMPILNLCRTSEFYYKDGDAYTFSKNNLKDVISMV 428

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TPSGD_VITVI3.0e-4849.29(-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1[more]
TPS3_RICCO5.1e-4837.15Probable terpene synthase 3 OS=Ricinus communis GN=TPS3 PE=3 SV=1[more]
TPS2_RICCO1.3e-4340.08Probable terpene synthase 2 OS=Ricinus communis GN=TPS2 PE=3 SV=1[more]
AFSY1_CUCME2.9e-4343.62Alpha-farnesene synthase OS=Cucumis melo PE=1 SV=1[more]
STS1_THAGA6.4e-4345.58Sesquiterpene synthase 1 OS=Thapsia garganica GN=STS1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1X6_CUCSA2.7e-7267.46Uncharacterized protein OS=Cucumis sativus GN=Csa_3G039850 PE=3 SV=1[more]
A0A0B4VFP0_CUCME5.3e-6863.16Sesquiterpene synthase Tps3 OS=Cucumis melo PE=2 SV=1[more]
A0A0A0L4K8_CUCSA3.8e-6657.20Uncharacterized protein OS=Cucumis sativus GN=Csa_3G041370 PE=3 SV=1[more]
A5BBY5_VITVI6.4e-6635.59Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021510 PE=3 SV=1[more]
A0A0A0L782_CUCSA1.3e-6348.04Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040850 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23960.11.4e-2733.33 terpene synthase 21[more]
AT1G48800.14.0e-2733.46 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT4G20210.12.0e-2629.82 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT4G20230.12.6e-2628.10 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT5G44630.19.9e-2633.48 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659096594|ref|XP_008449179.1|4.4e-7669.38PREDICTED: (-)-germacrene D synthase-like [Cucumis melo][more]
gi|778675684|ref|XP_011650453.1|3.9e-7267.46PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus][more]
gi|745672490|gb|AJD19683.1|7.6e-6863.16sesquiterpene synthase Tps3 [Cucumis melo][more]
gi|659096596|ref|XP_008449181.1|2.2e-6763.16PREDICTED: (-)-germacrene D synthase-like [Cucumis melo][more]
gi|970040273|ref|XP_015081514.1|6.4e-6736.07PREDICTED: probable terpene synthase 3 [Solanum pennellii][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0000287magnesium ion binding
GO:0016829lyase activity
GO:0010333terpene synthase activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR008949Isoprenoid_synthase_dom_sf
IPR008930Terpenoid_cyclase/PrenylTrfase
IPR005630Terpene_synthase_metal-bd
IPR001906Terpene_synth_N
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g07970.1Cp4.1LG08g07970.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001906Terpene synthase, N-terminal domainGENE3DG3DSA:1.50.30.10coord: 47..223
score: 1.8
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 26..198
score: 1.6
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 224..337
score: 1.2
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidunknownSSF48239Terpenoid cyclases/Protein prenyltransferasescoord: 22..222
score: 8.63
IPR008949Isoprenoid synthase domainGENE3DG3DSA:1.10.600.10coord: 224..414
score: 2.4
IPR008949Isoprenoid synthase domainunknownSSF48576Terpenoid synthasescoord: 220..413
score: 1.63
NoneNo IPR availablePANTHERPTHR31225FAMILY NOT NAMEDcoord: 10..415
score: 3.5E