Cp4.1LG08g07740 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG08g07740
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionE3 ubiquitin-protein ligase
LocationCp4.1LG08 : 6127099 .. 6127785 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTTAGCAGATTTGGCCACATTCCGCTGCAATCTCCACCGCTACGACGGTCCCGATCAGCTTCTCCAAATTCGAATAGGTCATTCAAGCCGTCTGCTCACCGGATCGCCGTTCTCCGGATATCCTTTCAATATCGTCCATGAAACTCTCCCTACTACAATGGCGGATGCTCAGTTCCCCCTTCCTTTGCGAGAGCTCGAAGATCCGTTATTCTGTCAAGCTTACATCAAGGAATTTCTGAGTTCTTACAACCTTGGTGCCACAATAAAAAACGCCATTGCTCGGAGGATTGCCTCGTTCGTCGTTCAAATGGCGTGCGGAAACTACGAGATGACATTCTACATCGTTGCTGAAATCGATTACATCCAGATGTTTTGGATGGACGACAGTGCGACTGCGGCGGAGGAGGAGGAGGATGAGGAGGAGGAGACGGAGATTTTTGACGACTTTTCGGGGAGGAGAGGGGCGCCGGAGACGGCGATAGAGAGGCTGAAGAAGGAGAAATTCGATGGATTTGGGGGAGAAGATTTGGGGAATTGTAGTGTGTGTTACGATGAGATGAAGGATGGCGGGGAAGAAGTGAGCAGAATCCCGTGCGGACATGTGTATCATAAATCTTGTGTTCTCACTTGGCTTCAAAGGAACAACTCATGTCCTCTGTGCAGAAACAAACTAGAAGCTTAA

mRNA sequence

ATGTCTTTAGCAGATTTGGCCACATTCCGCTGCAATCTCCACCGCTACGACGGTCCCGATCAGCTTCTCCAAATTCGAATAGGTCATTCAAGCCGTCTGCTCACCGGATCGCCGTTCTCCGGATATCCTTTCAATATCGTCCATGAAACTCTCCCTACTACAATGGCGGATGCTCAGTTCCCCCTTCCTTTGCGAGAGCTCGAAGATCCGTTATTCTGTCAAGCTTACATCAAGGAATTTCTGAGTTCTTACAACCTTGGTGCCACAATAAAAAACGCCATTGCTCGGAGGATTGCCTCGTTCGTCGTTCAAATGGCGTGCGGAAACTACGAGATGACATTCTACATCGTTGCTGAAATCGATTACATCCAGATGTTTTGGATGGACGACAGTGCGACTGCGGCGGAGGAGGAGGAGGATGAGGAGGAGGAGACGGAGATTTTTGACGACTTTTCGGGGAGGAGAGGGGCGCCGGAGACGGCGATAGAGAGGCTGAAGAAGGAGAAATTCGATGGATTTGGGGGAGAAGATTTGGGGAATTGTAGTGTGTGTTACGATGAGATGAAGGATGGCGGGGAAGAAGTGAGCAGAATCCCGTGCGGACATGTGTATCATAAATCTTGTGTTCTCACTTGGCTTCAAAGGAACAACTCATGTCCTCTGTGCAGAAACAAACTAGAAGCTTAA

Coding sequence (CDS)

ATGTCTTTAGCAGATTTGGCCACATTCCGCTGCAATCTCCACCGCTACGACGGTCCCGATCAGCTTCTCCAAATTCGAATAGGTCATTCAAGCCGTCTGCTCACCGGATCGCCGTTCTCCGGATATCCTTTCAATATCGTCCATGAAACTCTCCCTACTACAATGGCGGATGCTCAGTTCCCCCTTCCTTTGCGAGAGCTCGAAGATCCGTTATTCTGTCAAGCTTACATCAAGGAATTTCTGAGTTCTTACAACCTTGGTGCCACAATAAAAAACGCCATTGCTCGGAGGATTGCCTCGTTCGTCGTTCAAATGGCGTGCGGAAACTACGAGATGACATTCTACATCGTTGCTGAAATCGATTACATCCAGATGTTTTGGATGGACGACAGTGCGACTGCGGCGGAGGAGGAGGAGGATGAGGAGGAGGAGACGGAGATTTTTGACGACTTTTCGGGGAGGAGAGGGGCGCCGGAGACGGCGATAGAGAGGCTGAAGAAGGAGAAATTCGATGGATTTGGGGGAGAAGATTTGGGGAATTGTAGTGTGTGTTACGATGAGATGAAGGATGGCGGGGAAGAAGTGAGCAGAATCCCGTGCGGACATGTGTATCATAAATCTTGTGTTCTCACTTGGCTTCAAAGGAACAACTCATGTCCTCTGTGCAGAAACAAACTAGAAGCTTAA

Protein sequence

MSLADLATFRCNLHRYDGPDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLPTTMADAQFPLPLRELEDPLFCQAYIKEFLSSYNLGATIKNAIARRIASFVVQMACGNYEMTFYIVAEIDYIQMFWMDDSATAAEEEEDEEEETEIFDDFSGRRGAPETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRNKLEA
BLAST of Cp4.1LG08g07740 vs. Swiss-Prot
Match: CIP8_ARATH (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana GN=CIP8 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.4e-12
Identity = 36/78 (46.15%), Postives = 48/78 (61.54%), Query Frame = 1

Query: 153 GRRGAP---ETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCV 212
           GRRGAP   ++AIE L+  +     GE +  C+VC D M  G E   ++PCGH YH  C+
Sbjct: 226 GRRGAPPAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMG-ETGKKLPCGHCYHGDCI 285

Query: 213 LTWLQRNNSCPLCRNKLE 228
           + WL   NSCP+CR +LE
Sbjct: 286 VPWLGTRNSCPVCRFQLE 302

BLAST of Cp4.1LG08g07740 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.2e-10
Identity = 24/50 (48.00%), Postives = 38/50 (76.00%), Query Frame = 1

Query: 177 DLGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRNKL 227
           ++  C+VC DE +DG + V ++PC HV+H+ C+L WL+ +NSCP+CR +L
Sbjct: 212 EMNQCAVCMDEFEDGSD-VKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

BLAST of Cp4.1LG08g07740 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.7e-10
Identity = 23/50 (46.00%), Postives = 37/50 (74.00%), Query Frame = 1

Query: 177 DLGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRNKL 227
           +   C+VC D+ ++G  E  ++PC H+YHK C+L WL+ +NSCP+CR++L
Sbjct: 220 EFNQCAVCMDDFEEG-TEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHEL 268

BLAST of Cp4.1LG08g07740 vs. Swiss-Prot
Match: RHC1A_ARATH (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 36/89 (40.45%), Postives = 50/89 (56.18%), Query Frame = 1

Query: 146 EIFDDFSG---RRG---APETAIERLKKEKFDG--FGGEDLGNCSVCYDEMKDGGEEVSR 205
           E+F+  S    RRG   AP +AI+ L   K         D  NC VC DE  + G E  +
Sbjct: 148 ELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSD-SNCPVCKDEF-ELGSEAKQ 207

Query: 206 IPCGHVYHKSCVLTWLQRNNSCPLCRNKL 227
           +PC H+YH  C++ WL ++NSCP+CR +L
Sbjct: 208 MPCNHIYHSDCIVPWLVQHNSCPVCRQEL 234

BLAST of Cp4.1LG08g07740 vs. Swiss-Prot
Match: YGI4_SCHPO (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPBC2A9.04c PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.6e-09
Identity = 27/73 (36.99%), Postives = 46/73 (63.01%), Query Frame = 1

Query: 162 IERLKKEKFDGFGGEDLGN-------CSVCYDEMKDGGE-EVSRIPCGHVYHKSCVLTWL 221
           ++R  KE +D F  E L N       C +CYD+M +  E + +++PCGH++ K+C+  WL
Sbjct: 83  VKRAVKEAWDSF--EPLSNDQLMDLTCPICYDDMNENDEKQATKMPCGHIFGKNCLQKWL 142

Query: 222 QRNNSCPLCRNKL 227
           + + +CPLCR ++
Sbjct: 143 ENHCTCPLCRKEV 153

BLAST of Cp4.1LG08g07740 vs. TrEMBL
Match: A0A0A0LRB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 3.7e-39
Identity = 99/242 (40.91%), Postives = 145/242 (59.92%), Query Frame = 1

Query: 1   MSLADLATFRCNLH-RYDG-------PDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLP 60
           MS     +FRC++    +G       P Q +++R+GH S+L+  SP       I+ +T P
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVL-SP----SHQILQQTSP 60

Query: 61  TTMADAQFPLPLRELEDPLFCQ-AYIKEFLSSYNLGATIKNAIARRIASFVVQMACGNYE 120
             ++D     PLRELEDP F   + +  FLSSYN+   + +AI  +I+SF  Q+  GN  
Sbjct: 61  ILISDTLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLN 120

Query: 121 MTFYIVAEIDYIQMFWMD-DSATAAEEEEDEEEETEIFDDFSGRRGAPETAIERLKKEKF 180
             F+I+A +D++   W+D D A                D  + R+GAP +AIERL KEK+
Sbjct: 121 SNFHIIAALDFVYTHWVDLDPAE---------------DAAAVRQGAPRSAIERLMKEKY 180

Query: 181 DGFGGEDLGN-CSVCYDEMK---DGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRNKL 229
           DG GGE++ + CSVCY+++    +  +EVSRIPCGH++HKSC+L WL+ +NSCPLCR +L
Sbjct: 181 DGNGGEEMEDECSVCYEDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGEL 222

BLAST of Cp4.1LG08g07740 vs. TrEMBL
Match: A0A0A0K696_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 6.1e-34
Identity = 83/211 (39.34%), Postives = 123/211 (58.29%), Query Frame = 1

Query: 18  GPDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLPTTMADAQFPLPLRELEDPLFCQAYI 77
           G + +LQ+ I    ++++ SP +G P  ++H+T      +A F L LR+L+ PLF   YI
Sbjct: 19  GGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNALFYLSLRQLQSPLF---YI 78

Query: 78  KEFLSSYNLGATIKNAIARRIASFVVQMACGNYEMTFYIVAEIDYIQMFWMDDSATAAEE 137
            + LSS N+       +ARRIAS ++QM   N E  F+I+AE+D+I++ W+         
Sbjct: 79  TQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAG 138

Query: 138 EEDEEEETEIFDDF--SGRRGAPETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEV 197
            E    ET + D+   + +RG      ERL+ E+      E+LG+CS+C DE+     EV
Sbjct: 139 AE----ETVVLDEAPPAVKRGVGVARGERLRSEE----KMEELGDCSICLDELSCEKREV 198

Query: 198 SRIPCGHVYHKSCVLTWLQRNNSCPLCRNKL 227
            RIPCGHVYH+SC+  WL+ +NSCPLCR  L
Sbjct: 199 MRIPCGHVYHESCIFKWLENHNSCPLCRKPL 218

BLAST of Cp4.1LG08g07740 vs. TrEMBL
Match: A0A0A0K2W2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 6.1e-34
Identity = 83/211 (39.34%), Postives = 123/211 (58.29%), Query Frame = 1

Query: 18  GPDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLPTTMADAQFPLPLRELEDPLFCQAYI 77
           G + +LQ+ I    ++++ SP +G P  ++H+T      +A F L LR+L+ PLF   YI
Sbjct: 19  GGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNALFYLSLRQLQSPLF---YI 78

Query: 78  KEFLSSYNLGATIKNAIARRIASFVVQMACGNYEMTFYIVAEIDYIQMFWMDDSATAAEE 137
            + LSS N+       +ARRIAS ++QM   N E  F+I+AE+D+I++ W+         
Sbjct: 79  TQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAG 138

Query: 138 EEDEEEETEIFDDF--SGRRGAPETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEV 197
            E    ET + D+   + +RG      ERL+ E+      E+LG+CS+C DE+     EV
Sbjct: 139 AE----ETVVLDEAPPAVKRGVGVARGERLRSEE----KMEELGDCSICLDELSCEKREV 198

Query: 198 SRIPCGHVYHKSCVLTWLQRNNSCPLCRNKL 227
            RIPCGHVYH+SC+  WL+ +NSCPLCR  L
Sbjct: 199 MRIPCGHVYHESCIFKWLENHNSCPLCRKPL 218

BLAST of Cp4.1LG08g07740 vs. TrEMBL
Match: A0A0A0LHS2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.8e-33
Identity = 91/239 (38.08%), Postives = 133/239 (55.65%), Query Frame = 1

Query: 1   MSLADLATFRCNLHRYDGPDQLLQIRIGHSSRLLTGSP---------FSGYPFNIVHETL 60
           MS +  +  +C+   Y   DQ L +R+   SRL++ S              PF ++ E L
Sbjct: 1   MSFSATSCVQCSFSGYPNTDQTLIVRLRFCSRLISQSLAPEIVPDVILEQTPFTLIGEAL 60

Query: 61  PTTMADAQFPLPLRELEDPLFCQAYIKEFLSSYNLGATIKNAIARRIASFVVQMACGNYE 120
             ++++ Q+PL  +ELE        + EFL S+NL +++   ++  I SF  Q    N  
Sbjct: 61  -FSLSNLQYPLQQQELE------YMVNEFLRSFNLRSSVCRIVSHNILSFAQQFDINNE- 120

Query: 121 MTFYIVAEIDYIQMFWMDDSATAAEEEEDEEEETEIFDDFSGRRGAPETAIERLKKEKFD 180
             F I   +DYI+ +W        E   +    + + ++    RGA E+AIER+KK+KFD
Sbjct: 121 -CFKIETNVDYIKEYW-------TEAAPELYPISTVGEEGLPPRGASESAIERVKKQKFD 180

Query: 181 GFGGED---LGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRNKLE 228
           GF GE+   +G+CSVC +E+K  G EV RIPCGHVYHKSC+L WL+ +NSCPLCR  LE
Sbjct: 181 GFRGEEEEEIGDCSVCCEEIKRKGGEVRRIPCGHVYHKSCILKWLEISNSCPLCRAALE 223

BLAST of Cp4.1LG08g07740 vs. TrEMBL
Match: A0A0A0L3Z2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 5.2e-33
Identity = 95/249 (38.15%), Postives = 141/249 (56.63%), Query Frame = 1

Query: 1   MSLADLATFRCNLHRY-------DG-PDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLP 60
           MSL    +F+C++ R        DG P Q +Q+R+ H  +L+  +P       I+ +T P
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVH-TPSQ----QILQQTSP 60

Query: 61  TTMADAQFPLPLRELEDPLFCQAY-IKEFLSSYNLGATIKNAIARRIASFVVQM---ACG 120
           T ++D   P  L ELEDP F   + +  FLSSY++ A + +AI   I+SF  Q+   A  
Sbjct: 61  TLISDTLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADS 120

Query: 121 NYEMTFYIVAEIDYIQMFWMDDSATAAEEEEDEEEETEIFDDFSGRRGAPETAIERLKKE 180
           N  + F+I+A++D +   W+D      +  ED           + R+GA  + IER+ KE
Sbjct: 121 NINVNFHIIADVDIVYTHWVD-----LDPAEDAP---------AVRKGASTSVIERVMKE 180

Query: 181 KFDGFGGEDL---GNCSVCYDEM-------KDGGEEVSRIPCGHVYHKSCVLTWLQRNNS 228
           K+DG GGE+      CSVCY+++        +  +EVSRIPCGH+YHKSC+L WL+ +NS
Sbjct: 181 KYDGNGGEEKEGDDQCSVCYEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNS 230

BLAST of Cp4.1LG08g07740 vs. TAIR10
Match: AT5G64920.1 (AT5G64920.1 COP1-interacting protein 8)

HSP 1 Score: 72.0 bits (175), Expect = 5.3e-13
Identity = 36/78 (46.15%), Postives = 48/78 (61.54%), Query Frame = 1

Query: 153 GRRGAP---ETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCV 212
           GRRGAP   ++AIE L+  +     GE +  C+VC D M  G E   ++PCGH YH  C+
Sbjct: 226 GRRGAPPAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMG-ETGKKLPCGHCYHGDCI 285

Query: 213 LTWLQRNNSCPLCRNKLE 228
           + WL   NSCP+CR +LE
Sbjct: 286 VPWLGTRNSCPVCRFQLE 302

BLAST of Cp4.1LG08g07740 vs. TAIR10
Match: AT1G60360.1 (AT1G60360.1 RING/U-box superfamily protein)

HSP 1 Score: 66.6 bits (161), Expect = 2.2e-11
Identity = 32/79 (40.51%), Postives = 43/79 (54.43%), Query Frame = 1

Query: 149 DDFSGRRGAPETAIERLKKEKFDGFG-GEDLGNCSVCYDEMKDGGEEVSRIPCGHVYHKS 208
           DD  G   A E  I  L   K        D+  C+VC +E   GG+  + +PC H+YHK 
Sbjct: 191 DDRPGPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGGD-ATELPCKHIYHKD 250

Query: 209 CVLTWLQRNNSCPLCRNKL 227
           C++ WL+ NNSCP+CR  L
Sbjct: 251 CIVPWLRLNNSCPICRRDL 268

BLAST of Cp4.1LG08g07740 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 66.2 bits (160), Expect = 2.9e-11
Identity = 24/50 (48.00%), Postives = 38/50 (76.00%), Query Frame = 1

Query: 177 DLGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRNKL 227
           ++  C+VC DE +DG + V ++PC HV+H+ C+L WL+ +NSCP+CR +L
Sbjct: 212 EMNQCAVCMDEFEDGSD-VKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

BLAST of Cp4.1LG08g07740 vs. TAIR10
Match: AT5G41430.1 (AT5G41430.1 RING/U-box superfamily protein)

HSP 1 Score: 65.5 bits (158), Expect = 5.0e-11
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 1

Query: 154 RRGAPETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCVLTWL 213
           R    E   + ++KE FD  G      CS+C +E++DG E +    C HV+H+SC+ +WL
Sbjct: 96  RASIEEMEFKDIEKEGFDEIG------CSICLEELEDGHEIIRIKKCRHVFHRSCIDSWL 155

Query: 214 QRNNSCPLCR 224
           ++N SCP CR
Sbjct: 156 KQNRSCPNCR 159

BLAST of Cp4.1LG08g07740 vs. TAIR10
Match: AT2G40830.2 (AT2G40830.2 RING-H2 finger C1A)

HSP 1 Score: 65.1 bits (157), Expect = 6.5e-11
Identity = 36/89 (40.45%), Postives = 50/89 (56.18%), Query Frame = 1

Query: 146 EIFDDFSG---RRG---APETAIERLKKEKFDG--FGGEDLGNCSVCYDEMKDGGEEVSR 205
           E+F+  S    RRG   AP +AI+ L   K         D  NC VC DE  + G E  +
Sbjct: 148 ELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSD-SNCPVCKDEF-ELGSEAKQ 207

Query: 206 IPCGHVYHKSCVLTWLQRNNSCPLCRNKL 227
           +PC H+YH  C++ WL ++NSCP+CR +L
Sbjct: 208 MPCNHIYHSDCIVPWLVQHNSCPVCRQEL 234

BLAST of Cp4.1LG08g07740 vs. NCBI nr
Match: gi|778674460|ref|XP_011650219.1| (PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus])

HSP 1 Score: 169.9 bits (429), Expect = 5.3e-39
Identity = 99/242 (40.91%), Postives = 145/242 (59.92%), Query Frame = 1

Query: 1   MSLADLATFRCNLH-RYDG-------PDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLP 60
           MS     +FRC++    +G       P Q +++R+GH S+L+  SP       I+ +T P
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVL-SP----SHQILQQTSP 60

Query: 61  TTMADAQFPLPLRELEDPLFCQ-AYIKEFLSSYNLGATIKNAIARRIASFVVQMACGNYE 120
             ++D     PLRELEDP F   + +  FLSSYN+   + +AI  +I+SF  Q+  GN  
Sbjct: 61  ILISDTLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLN 120

Query: 121 MTFYIVAEIDYIQMFWMD-DSATAAEEEEDEEEETEIFDDFSGRRGAPETAIERLKKEKF 180
             F+I+A +D++   W+D D A                D  + R+GAP +AIERL KEK+
Sbjct: 121 SNFHIIAALDFVYTHWVDLDPAE---------------DAAAVRQGAPRSAIERLMKEKY 180

Query: 181 DGFGGEDLGN-CSVCYDEMK---DGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRNKL 229
           DG GGE++ + CSVCY+++    +  +EVSRIPCGH++HKSC+L WL+ +NSCPLCR +L
Sbjct: 181 DGNGGEEMEDECSVCYEDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGEL 222

BLAST of Cp4.1LG08g07740 vs. NCBI nr
Match: gi|449454422|ref|XP_004144954.1| (PREDICTED: uncharacterized protein LOC101214496 [Cucumis sativus])

HSP 1 Score: 152.5 bits (384), Expect = 8.7e-34
Identity = 83/211 (39.34%), Postives = 123/211 (58.29%), Query Frame = 1

Query: 18  GPDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLPTTMADAQFPLPLRELEDPLFCQAYI 77
           G + +LQ+ I    ++++ SP +G P  ++H+T      +A F L LR+L+ PLF   YI
Sbjct: 19  GGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNALFYLSLRQLQSPLF---YI 78

Query: 78  KEFLSSYNLGATIKNAIARRIASFVVQMACGNYEMTFYIVAEIDYIQMFWMDDSATAAEE 137
            + LSS N+       +ARRIAS ++QM   N E  F+I+AE+D+I++ W+         
Sbjct: 79  TQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAG 138

Query: 138 EEDEEEETEIFDDF--SGRRGAPETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEV 197
            E    ET + D+   + +RG      ERL+ E+      E+LG+CS+C DE+     EV
Sbjct: 139 AE----ETVVLDEAPPAVKRGVGVARGERLRSEE----KMEELGDCSICLDELSCEKREV 198

Query: 198 SRIPCGHVYHKSCVLTWLQRNNSCPLCRNKL 227
            RIPCGHVYH+SC+  WL+ +NSCPLCR  L
Sbjct: 199 MRIPCGHVYHESCIFKWLENHNSCPLCRKPL 218

BLAST of Cp4.1LG08g07740 vs. NCBI nr
Match: gi|778730084|ref|XP_011659694.1| (PREDICTED: uncharacterized protein LOC105436225 [Cucumis sativus])

HSP 1 Score: 152.5 bits (384), Expect = 8.7e-34
Identity = 83/211 (39.34%), Postives = 123/211 (58.29%), Query Frame = 1

Query: 18  GPDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLPTTMADAQFPLPLRELEDPLFCQAYI 77
           G + +LQ+ I    ++++ SP +G P  ++H+T      +A F L LR+L+ PLF   YI
Sbjct: 19  GGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNALFYLSLRQLQSPLF---YI 78

Query: 78  KEFLSSYNLGATIKNAIARRIASFVVQMACGNYEMTFYIVAEIDYIQMFWMDDSATAAEE 137
            + LSS N+       +ARRIAS ++QM   N E  F+I+AE+D+I++ W+         
Sbjct: 79  TQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAG 138

Query: 138 EEDEEEETEIFDDF--SGRRGAPETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEV 197
            E    ET + D+   + +RG      ERL+ E+      E+LG+CS+C DE+     EV
Sbjct: 139 AE----ETVVLDEAPPAVKRGVGVARGERLRSEE----KMEELGDCSICLDELSCEKREV 198

Query: 198 SRIPCGHVYHKSCVLTWLQRNNSCPLCRNKL 227
            RIPCGHVYH+SC+  WL+ +NSCPLCR  L
Sbjct: 199 MRIPCGHVYHESCIFKWLENHNSCPLCRKPL 218

BLAST of Cp4.1LG08g07740 vs. NCBI nr
Match: gi|449442467|ref|XP_004139003.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus])

HSP 1 Score: 151.0 bits (380), Expect = 2.5e-33
Identity = 91/239 (38.08%), Postives = 133/239 (55.65%), Query Frame = 1

Query: 1   MSLADLATFRCNLHRYDGPDQLLQIRIGHSSRLLTGSP---------FSGYPFNIVHETL 60
           MS +  +  +C+   Y   DQ L +R+   SRL++ S              PF ++ E L
Sbjct: 1   MSFSATSCVQCSFSGYPNTDQTLIVRLRFCSRLISQSLAPEIVPDVILEQTPFTLIGEAL 60

Query: 61  PTTMADAQFPLPLRELEDPLFCQAYIKEFLSSYNLGATIKNAIARRIASFVVQMACGNYE 120
             ++++ Q+PL  +ELE        + EFL S+NL +++   ++  I SF  Q    N  
Sbjct: 61  -FSLSNLQYPLQQQELE------YMVNEFLRSFNLRSSVCRIVSHNILSFAQQFDINNE- 120

Query: 121 MTFYIVAEIDYIQMFWMDDSATAAEEEEDEEEETEIFDDFSGRRGAPETAIERLKKEKFD 180
             F I   +DYI+ +W        E   +    + + ++    RGA E+AIER+KK+KFD
Sbjct: 121 -CFKIETNVDYIKEYW-------TEAAPELYPISTVGEEGLPPRGASESAIERVKKQKFD 180

Query: 181 GFGGED---LGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRNKLE 228
           GF GE+   +G+CSVC +E+K  G EV RIPCGHVYHKSC+L WL+ +NSCPLCR  LE
Sbjct: 181 GFRGEEEEEIGDCSVCCEEIKRKGGEVRRIPCGHVYHKSCILKWLEISNSCPLCRAALE 223

BLAST of Cp4.1LG08g07740 vs. NCBI nr
Match: gi|700199693|gb|KGN54851.1| (hypothetical protein Csa_4G551140 [Cucumis sativus])

HSP 1 Score: 149.4 bits (376), Expect = 7.4e-33
Identity = 95/249 (38.15%), Postives = 141/249 (56.63%), Query Frame = 1

Query: 1   MSLADLATFRCNLHRY-------DG-PDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLP 60
           MSL    +F+C++ R        DG P Q +Q+R+ H  +L+  +P       I+ +T P
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVH-TPSQ----QILQQTSP 60

Query: 61  TTMADAQFPLPLRELEDPLFCQAY-IKEFLSSYNLGATIKNAIARRIASFVVQM---ACG 120
           T ++D   P  L ELEDP F   + +  FLSSY++ A + +AI   I+SF  Q+   A  
Sbjct: 61  TLISDTLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADS 120

Query: 121 NYEMTFYIVAEIDYIQMFWMDDSATAAEEEEDEEEETEIFDDFSGRRGAPETAIERLKKE 180
           N  + F+I+A++D +   W+D      +  ED           + R+GA  + IER+ KE
Sbjct: 121 NINVNFHIIADVDIVYTHWVD-----LDPAEDAP---------AVRKGASTSVIERVMKE 180

Query: 181 KFDGFGGEDL---GNCSVCYDEM-------KDGGEEVSRIPCGHVYHKSCVLTWLQRNNS 228
           K+DG GGE+      CSVCY+++        +  +EVSRIPCGH+YHKSC+L WL+ +NS
Sbjct: 181 KYDGNGGEEKEGDDQCSVCYEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNS 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CIP8_ARATH9.4e-1246.15E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana GN=CIP8 PE=1 SV=1[more]
RNG1L_ARATH5.2e-1048.00E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1... [more]
RING1_GOSHI6.7e-1046.00E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1[more]
RHC1A_ARATH1.1e-0940.45Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2... [more]
YGI4_SCHPO2.6e-0936.99Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strai... [more]
Match NameE-valueIdentityDescription
A0A0A0LRB0_CUCSA3.7e-3940.91Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1[more]
A0A0A0K696_CUCSA6.1e-3439.34Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1[more]
A0A0A0K2W2_CUCSA6.1e-3439.34Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1[more]
A0A0A0LHS2_CUCSA1.8e-3338.08Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1[more]
A0A0A0L3Z2_CUCSA5.2e-3338.15Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G64920.15.3e-1346.15 COP1-interacting protein 8[more]
AT1G60360.12.2e-1140.51 RING/U-box superfamily protein[more]
AT3G19950.12.9e-1148.00 RING/U-box superfamily protein[more]
AT5G41430.15.0e-1140.00 RING/U-box superfamily protein[more]
AT2G40830.26.5e-1140.45 RING-H2 finger C1A[more]
Match NameE-valueIdentityDescription
gi|778674460|ref|XP_011650219.1|5.3e-3940.91PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus][more]
gi|449454422|ref|XP_004144954.1|8.7e-3439.34PREDICTED: uncharacterized protein LOC101214496 [Cucumis sativus][more]
gi|778730084|ref|XP_011659694.1|8.7e-3439.34PREDICTED: uncharacterized protein LOC105436225 [Cucumis sativus][more]
gi|449442467|ref|XP_004139003.1|2.5e-3338.08PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus][more]
gi|700199693|gb|KGN54851.1|7.4e-3338.15hypothetical protein Csa_4G551140 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g07740.1Cp4.1LG08g07740.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 180..223
score: 2.4
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 181..222
score: 4.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 181..223
score: 13
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 179..226
score: 1.8
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 145..227
score: 1.6
NoneNo IPR availablePANTHERPTHR22763:SF39RING/U-BOX DOMAIN-CONTAINING PROTEINcoord: 145..227
score: 1.6
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 180..226
score: 1.66E-19coord: 140..145
score: 1.66

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG08g07740Cucurbita pepo (Zucchini)cpecpeB251
Cp4.1LG08g07740Cucurbita pepo (Zucchini)cpecpeB230
Cp4.1LG08g07740Cucurbita pepo (Zucchini)cpecpeB508
Cp4.1LG08g07740Cucumber (Gy14) v1cgycpeB0796
Cp4.1LG08g07740Cucumber (Gy14) v1cgycpeB0857
Cp4.1LG08g07740Cucurbita maxima (Rimu)cmacpeB336
Cp4.1LG08g07740Cucurbita maxima (Rimu)cmacpeB365
Cp4.1LG08g07740Cucurbita maxima (Rimu)cmacpeB649
Cp4.1LG08g07740Cucurbita maxima (Rimu)cmacpeB852
Cp4.1LG08g07740Cucurbita moschata (Rifu)cmocpeB300
Cp4.1LG08g07740Cucurbita moschata (Rifu)cmocpeB329
Cp4.1LG08g07740Cucurbita moschata (Rifu)cmocpeB598
Cp4.1LG08g07740Cucurbita moschata (Rifu)cmocpeB601
Cp4.1LG08g07740Cucurbita moschata (Rifu)cmocpeB797
Cp4.1LG08g07740Wild cucumber (PI 183967)cpecpiB852
Cp4.1LG08g07740Wild cucumber (PI 183967)cpecpiB874
Cp4.1LG08g07740Cucumber (Chinese Long) v2cpecuB850
Cp4.1LG08g07740Cucumber (Chinese Long) v2cpecuB872
Cp4.1LG08g07740Bottle gourd (USVL1VR-Ls)cpelsiB698
Cp4.1LG08g07740Bottle gourd (USVL1VR-Ls)cpelsiB721
Cp4.1LG08g07740Watermelon (Charleston Gray)cpewcgB766
Cp4.1LG08g07740Watermelon (Charleston Gray)cpewcgB780
Cp4.1LG08g07740Watermelon (97103) v1cpewmB828
Cp4.1LG08g07740Watermelon (97103) v1cpewmB846
Cp4.1LG08g07740Melon (DHL92) v3.5.1cpemeB790
Cp4.1LG08g07740Melon (DHL92) v3.5.1cpemeB814
Cp4.1LG08g07740Cucumber (Gy14) v2cgybcpeB445
Cp4.1LG08g07740Cucumber (Gy14) v2cgybcpeB702
Cp4.1LG08g07740Melon (DHL92) v3.6.1cpemedB928
Cp4.1LG08g07740Melon (DHL92) v3.6.1cpemedB948
Cp4.1LG08g07740Silver-seed gourdcarcpeB1206
Cp4.1LG08g07740Silver-seed gourdcarcpeB1211
Cp4.1LG08g07740Silver-seed gourdcarcpeB1299
Cp4.1LG08g07740Cucumber (Chinese Long) v3cpecucB1047
Cp4.1LG08g07740Cucumber (Chinese Long) v3cpecucB1070
Cp4.1LG08g07740Wax gourdcpewgoB1101
Cp4.1LG08g07740Wax gourdcpewgoB1104