Cp4.1LG08g05730 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG08g05730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCp4.1LG08 : 676815 .. 678541 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCATACAACTTTGAAGCTCAATTCAATTCCCAACCACTTCACGTTTTCCGACACAATTCGCTCCAATCCCCACTCCTTCGGCCAAGTTTCGCTCCCCAAAAGATCCGATAATGTTTCTTCTTCTTCTACCGCCGCCGCCGCCATGGTTCACAAGAACGCCGGGATGACAAGCTTGTGGCGTCAGATTCACGGCTGCAATAACTGGGAAGATCTGGTGGAACCTAATCTTCATCCACTTTTACGGCGGGAAGTTATCCGGTACGGCGAGTTTGTGGCGGCGTGTTATAAAGCTTTTGATCTTGATCCAAATTCCAAACGGTATTTAACTTGCAAATTTGGAAAGAAGAATTTGTTTAAGGAAGTAGGGTTGGAGAATTCCGGTTATGAAGTGTCTAAATACATCTATGCTACCCCTCCCTTCATCAATATTCCTCCCATCCAAAACTCCCCACCGGCTTGCGGACGGTGGATCGGCTACGTCGCCGTCTCCTCCGATTATATGAGTACGAGACTCGGTCGACGAGACATTGTTATCACTTTCCGAGGTACCGTAACGATCCCTGAATGGATTGCGATTCTAATGAGCTCCCTCACACCTGCTCACCTCGACTGTTACAATTATCGCCCCGATGTCAAGGTTTTTAATCCACCCAAAAGCTTAATTTTTTTTTTTTTTATTAGTAATCGCATGACATACATTCTTATATTTTCCAGGTCGTGTCCTATCTGGGTTTCTAACGCTTTACTCAATCAACCCAAAAGCTTAAATTTTTGTAACTATTTAATAATCGAGTGACATATATTTTTACATTTCCCAGGTCGTATCTGGGTTTCTAACGCTTTACTCAATCAACCCAAAAGCTTAAATTTTTTGAACTATTTAGTAACCGAGTGATTTGTATTCTTACATTTTCCAGATCGTATTGTATCTGGGTTCTAACGCTTTACTCAATCAGTCCAAAAGCTTAAATTTTTTTGTATTTAGTGATCGAATCGATTGACATATATTCTTACATTTTCCCGGTCGTATCTGGGTTTCTAACGCTTTACTCAACCCAAACGCTTAAATTTTTTTAACTATTTAGTAATCGAGTAACATATATTCTTACATTTTTCCAGTCGTATTCTATCTGGGTTTCTAATGCTTTACTCAATCAATCCAAAAGCTTAATTTTTTTTTGTAACTATTTAGTAATCAAGTGACTTATATTCTTACATTTTTCAGATGGGTTTCTAACGCTTTACTCAATTAGTCCAAAAGCTTAAATTTTTTTTGTAACTATTTAGTAATCGAGTGATATATATTCTTAATTTTTTCAGGTCCTATCTGGGTTTCTAACGCTCTACACGTGCAATGAAAAGTGCACGAAATTCGGGCTCGAGAGCTGCCGGGAGCAGCTGCTCTCGGAAGTGTCGAGGCTATTAAACAAGTACAAGAACGAAGAGGTGAGTATAACCATGGCAGGGCATAGTATGGGAAGTGCATTAGGCGTTCTTCTAGCATATGACATAGCTGAGCTAGGATTGAACAAAAGAACAAACAAAGAAGCTGTTCCAATTTCAGTGTTTTCCTTCGGAGGTCCAAAAGTTGGGAATTTAAACTTCAAACAAAGATGCGAAGAATTGGGAGTAAAAGTTTTGAGAATCGTGAACGTGAACGACCCGATCACAAAAATGCCAGGCGTTTTGTTCAATAGCTATGAACACGTTGGAGTTGAATTG

mRNA sequence

ATGGCGCATACAACTTTGAAGCTCAATTCAATTCCCAACCACTTCACGTTTTCCGACACAATTCGCTCCAATCCCCACTCCTTCGGCCAAGTTTCGCTCCCCAAAAGATCCGATAATGTTTCTTCTTCTTCTACCGCCGCCGCCGCCATGGTTCACAAGAACGCCGGGATGACAAGCTTGTGGCGTCAGATTCACGGCTGCAATAACTGGGAAGATCTGGTGGAACCTAATCTTCATCCACTTTTACGGCGGGAAGTTATCCGGTACGGCGAGTTTGTGGCGGCGTGTTATAAAGCTTTTGATCTTGATCCAAATTCCAAACGGTATTTAACTTGCAAATTTGGAAAGAAGAATTTGTTTAAGGAAGTAGGGTTGGAGAATTCCGGTTATGAAGTGTCTAAATACATCTATGCTACCCCTCCCTTCATCAATATTCCTCCCATCCAAAACTCCCCACCGGCTTGCGGACGGTGGATCGGCTACGTCGCCGTCTCCTCCGATTATATGAGTACGAGACTCGGTCGACGAGACATTGTTATCACTTTCCGAGGTACCGTAACGATCCCTGAATGGATTGCGATTCTAATGAGCTCCCTCACACCTGCTCACCTCGACTGTTACAATTATCGCCCCGATGTCAAGGTCCTATCTGGGTTTCTAACGCTCTACACGTGCAATGAAAAGTGCACGAAATTCGGGCTCGAGAGCTGCCGGGAGCAGCTGCTCTCGGAAGTGTCGAGGCTATTAAACAAGTACAAGAACGAAGAGGTGAGTATAACCATGGCAGGGCATAGTATGGGAAGTGCATTAGGCGTTCTTCTAGCATATGACATAGCTGAGCTAGGATTGAACAAAAGAACAAACAAAGAAGCTGTTCCAATTTCAGTGTTTTCCTTCGGAGGTCCAAAAGTTGGGAATTTAAACTTCAAACAAAGATGCGAAGAATTGGGAGTAAAAGTTTTGAGAATCGTGAACGTGAACGACCCGATCACAAAAATGCCAGGCGTTTTGTTCAATAGCTATGAACACGTTGGAGTTGAATTG

Coding sequence (CDS)

ATGGCGCATACAACTTTGAAGCTCAATTCAATTCCCAACCACTTCACGTTTTCCGACACAATTCGCTCCAATCCCCACTCCTTCGGCCAAGTTTCGCTCCCCAAAAGATCCGATAATGTTTCTTCTTCTTCTACCGCCGCCGCCGCCATGGTTCACAAGAACGCCGGGATGACAAGCTTGTGGCGTCAGATTCACGGCTGCAATAACTGGGAAGATCTGGTGGAACCTAATCTTCATCCACTTTTACGGCGGGAAGTTATCCGGTACGGCGAGTTTGTGGCGGCGTGTTATAAAGCTTTTGATCTTGATCCAAATTCCAAACGGTATTTAACTTGCAAATTTGGAAAGAAGAATTTGTTTAAGGAAGTAGGGTTGGAGAATTCCGGTTATGAAGTGTCTAAATACATCTATGCTACCCCTCCCTTCATCAATATTCCTCCCATCCAAAACTCCCCACCGGCTTGCGGACGGTGGATCGGCTACGTCGCCGTCTCCTCCGATTATATGAGTACGAGACTCGGTCGACGAGACATTGTTATCACTTTCCGAGGTACCGTAACGATCCCTGAATGGATTGCGATTCTAATGAGCTCCCTCACACCTGCTCACCTCGACTGTTACAATTATCGCCCCGATGTCAAGGTCCTATCTGGGTTTCTAACGCTCTACACGTGCAATGAAAAGTGCACGAAATTCGGGCTCGAGAGCTGCCGGGAGCAGCTGCTCTCGGAAGTGTCGAGGCTATTAAACAAGTACAAGAACGAAGAGGTGAGTATAACCATGGCAGGGCATAGTATGGGAAGTGCATTAGGCGTTCTTCTAGCATATGACATAGCTGAGCTAGGATTGAACAAAAGAACAAACAAAGAAGCTGTTCCAATTTCAGTGTTTTCCTTCGGAGGTCCAAAAGTTGGGAATTTAAACTTCAAACAAAGATGCGAAGAATTGGGAGTAAAAGTTTTGAGAATCGTGAACGTGAACGACCCGATCACAAAAATGCCAGGCGTTTTGTTCAATAGCTATGAACACGTTGGAGTTGAATTG

Protein sequence

MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSTAAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNSYEHVGVEL
BLAST of Cp4.1LG08g05730 vs. Swiss-Prot
Match: PLA13_ARATH (Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana GN=At2g31690 PE=1 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 5.5e-112
Identity = 215/390 (55.13%), Postives = 259/390 (66.41%), Query Frame = 1

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSTAAAAMVHKNAGMTS- 60
           M H     N  P    F     S P S+      K +++ SSSS  A  +++     +S 
Sbjct: 9   MKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSSSSSSLLAPVILNSPVASSSP 68

Query: 61  ----------------------LWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACY 120
                                 +WR+I GCNNW+DL+EP L+PLL++E+ RYG  V+ CY
Sbjct: 69  PPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITRYGNLVSTCY 128

Query: 121 KAFDLDPNSKRYLTCKFGKKNLFKEVGLEN-SGYEVSKYIYATPPF-INIPPIQNSPPAC 180
           KAFDLDPNSKRYL CK+GK+ L KE  ++    Y+V+KYIYATP   INI PIQN     
Sbjct: 129 KAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININISPIQNEMNRR 188

Query: 181 GRWIGYVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKV 240
            RW+GYVA SSD    RLGRRDIV+TFRGTVT PEW+A  MSSLTPA    +N R DVKV
Sbjct: 189 ARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHNPRLDVKV 248

Query: 241 LSGFLTLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLA 300
            SGFL+LYT +E  +KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSMGS+L  LLA
Sbjct: 249 ESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGSSLAQLLA 308

Query: 301 YDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPG 349
           YDIAELGLN+R  K  +P++VFSF GP+VGNL FK+RCEELGVKVLRI NVNDP+TK+PG
Sbjct: 309 YDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVNDPVTKLPG 368

BLAST of Cp4.1LG08g05730 vs. Swiss-Prot
Match: PLA12_ARATH (Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana GN=DGL PE=1 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 7.1e-112
Identity = 210/349 (60.17%), Postives = 258/349 (73.93%), Query Frame = 1

Query: 18  SDTIRSNPHSFGQVSLPKRSDNVSSSSTAAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPN 77
           S +  S P S   +SLP  S + +   + A A+      ++ +WR+I G NNWE+L+EP 
Sbjct: 44  SSSTMSPPISSSPLSLPSSSSSQAIPPSRAPAVT---LPLSRVWREIQGSNNWENLIEP- 103

Query: 78  LHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLEN-SGYEVSKYI 137
           L P+L++E+ RYG  ++A YK FDL+PNSKRYL+CK+GKKNL KE G+ +  GY+V+KYI
Sbjct: 104 LSPILQQEITRYGNLLSASYKGFDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYI 163

Query: 138 YATPPFINIPPIQNSPPACGRWIGYVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILM 197
           YATP  IN+ PI+N P    RWIGYVAVSSD    RLGRRDI++TFRGTVT  EW+A L 
Sbjct: 164 YATPD-INLNPIKNEPNRA-RWIGYVAVSSDESVKRLGRRDILVTFRGTVTNHEWLANLK 223

Query: 198 SSLTPAHLDCYNYRPDVKVLSGFLTLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEE 257
           SSLTPA LD +N RPDVKV SGFL LYT  E  +KFGLESCREQLLSE+SRL+NK+K EE
Sbjct: 224 SSLTPARLDPHNPRPDVKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKHKGEE 283

Query: 258 VSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEEL 317
           +SIT+AGHSMGS+L  LLAYDIAELG+N+R +++ VP++VFSF GP+VGNL FK+RCEEL
Sbjct: 284 ISITLAGHSMGSSLAQLLAYDIAELGMNQRRDEKPVPVTVFSFAGPRVGNLGFKKRCEEL 343

Query: 318 GVKVLRIVNVNDPITKMPGVLFNS-----------------YEHVGVEL 349
           GVKVLRI NVNDPITK+PG LFN                  Y HVGVEL
Sbjct: 344 GVKVLRITNVNDPITKLPGFLFNENFRSLGGVYELPWSCSCYTHVGVEL 386

BLAST of Cp4.1LG08g05730 vs. Swiss-Prot
Match: EG1_ORYSI (Phospholipase A1 EG1, chloroplastic OS=Oryza sativa subsp. indica GN=EG1 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.6e-98
Identity = 178/308 (57.79%), Postives = 225/308 (73.05%), Query Frame = 1

Query: 57  MTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGK 116
           +  +WR++ GC +WE ++EP  HP+LR EV RYGE V ACYKAFDLDP S+RYL CK+G+
Sbjct: 56  VAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFDLDPASRRYLNCKYGR 115

Query: 117 KNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYVAVSSDYMSTRLGRR 176
           + + +EVG+  +GYEV++YIYA    +++P ++ S    GRWIGYVAVS+D MS RLGRR
Sbjct: 116 ERMLEEVGMGGAGYEVTRYIYAAAD-VSVPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRR 175

Query: 177 DIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYTCNEKCTKF-GLE 236
           D++++FRGTVT  EW+A LMSSL  A LD  + RPDVKV SGFL+LYT  +K  +F G  
Sbjct: 176 DVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLSLYTSADKTCRFGGAG 235

Query: 237 SCREQLLSEVSRLLNKYK--NEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVP 296
           SCREQLL EVSRL+  Y    E+VS+T+AGHSMGSAL +L AYD+AELGLN+     A P
Sbjct: 236 SCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAYDLAELGLNR-----AAP 295

Query: 297 ISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS------------- 349
           ++VFSFGGP+VGN  FK RC+ELGVK LR+ NV+DPITK+PGV  N              
Sbjct: 296 VTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFLNEATAGVLRPWRHSC 355

BLAST of Cp4.1LG08g05730 vs. Swiss-Prot
Match: EG1_ORYSJ (Phospholipase A1 EG1, chloroplastic OS=Oryza sativa subsp. japonica GN=EG1 PE=1 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.6e-98
Identity = 178/308 (57.79%), Postives = 225/308 (73.05%), Query Frame = 1

Query: 57  MTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGK 116
           +  +WR++ GC +WE ++EP  HP+LR EV RYGE V ACYKAFDLDP S+RYL CK+G+
Sbjct: 56  VAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFDLDPASRRYLNCKYGR 115

Query: 117 KNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYVAVSSDYMSTRLGRR 176
           + + +EVG+  +GYEV++YIYA    +++P ++ S    GRWIGYVAVS+D MS RLGRR
Sbjct: 116 ERMLEEVGMGGAGYEVTRYIYAAAD-VSVPTMEPSTSGRGRWIGYVAVSTDEMSRRLGRR 175

Query: 177 DIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYTCNEKCTKF-GLE 236
           D++++FRGTVT  EW+A LMSSL  A LD  + RPDVKV SGFL+LYT  +K  +F G  
Sbjct: 176 DVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLSLYTSADKTCRFGGAG 235

Query: 237 SCREQLLSEVSRLLNKYK--NEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVP 296
           SCREQLL EVSRL+  Y    E+VS+T+AGHSMGSAL +L AYD+AELGLN+     A P
Sbjct: 236 SCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAYDLAELGLNR-----AAP 295

Query: 297 ISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS------------- 349
           ++VFSFGGP+VGN  FK RC+ELGVK LR+ NV+DPITK+PGV  N              
Sbjct: 296 VTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFLNEATAGVLRPWRHSC 355

BLAST of Cp4.1LG08g05730 vs. Swiss-Prot
Match: PLA17_ARATH (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana GN=At1g51440 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.4e-70
Identity = 148/326 (45.40%), Postives = 197/326 (60.43%), Query Frame = 1

Query: 53  KNAGMTSLWRQIHGCNNWEDLVEP-NLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLT 112
           K   +  +WR++ GCNNWE  ++P N H  LRRE+IRYGEF  ACY +FD DP+SK   +
Sbjct: 83  KEVSLREIWREVQGCNNWEGQLDPMNNH--LRREIIRYGEFAQACYDSFDFDPHSKYCGS 142

Query: 113 CKFGKKNLFKEVGLE-NSGYEVSKYIYAT-----PPFINIPPIQNSPPACGRWIGYVAVS 172
           CK+   + F  + L  + GY +++Y+YAT     P F     + +       W+G+VAV+
Sbjct: 143 CKYHPSDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVA 202

Query: 173 SDYMS-TRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYT 232
           +D    +RLGRRDIVI +RGTVT  EWI  L   L  A+   +   P +K+  GF  LYT
Sbjct: 203 TDEEEVSRLGRRDIVIAWRGTVTYLEWIYDLKDILCSAN---FGDDPSIKIELGFHDLYT 262

Query: 233 CNEKCTKFGLESCREQLLSEVSRLLNKYKNEE----VSITMAGHSMGSALGVLLAYDIAE 292
             E   KF   S REQ+L+EV RL+  Y  EE     SIT+ GHS+G++L ++ AYDIAE
Sbjct: 263 KKEDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAE 322

Query: 293 LGLNK-RTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFN 349
           L LN    N   +PI+VFSF GP+VGNL FK+RC+ELGVKVLR+VNV+D +  +PG+  N
Sbjct: 323 LNLNHVPENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTN 382

BLAST of Cp4.1LG08g05730 vs. TrEMBL
Match: A0A0A0L8A7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G174570 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 1.7e-157
Identity = 291/402 (72.39%), Postives = 311/402 (77.36%), Query Frame = 1

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKR-SDNVSSSST-------------- 60
           MA TTLKLNSIPNH TF +T R   HSFGQVS+P++   NVSSSS+              
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  -----------------------AAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLL 120
                                  AAAAMV K A +  LWRQIHGCN+WEDLVEP LHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPF 180
           RRE+IRYGEFV ACYKAFDLDPNSKRYLTCKFGKK+L KEVGLE+SGYEV+KYIYATPP 
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPACGRWIGYVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPA 240
           INIPPIQNSPP+CGRWIGYVAVSSD  S RLGRRDI+ITFRGTVT PEWIA LMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 HLDCYNYRPDVKVLSGFLTLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMA 300
            LD +N+RPDVKV SGFLTLYT  E   KFGLESCREQLLSEVSRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLR 349
           GHSMGSAL +LLAYDIAELGLN+RTN E VP+SVFSFGGP+VGN  FK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

BLAST of Cp4.1LG08g05730 vs. TrEMBL
Match: W9SFC4_9ROSA (Galactolipase DONGLE OS=Morus notabilis GN=L484_011039 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 1.7e-128
Identity = 244/387 (63.05%), Postives = 285/387 (73.64%), Query Frame = 1

Query: 5   TLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDN------------------------V 64
           TL   +  +H  FSD      HSFGQVSLP ++++                        +
Sbjct: 8   TLNTKTNNHHIIFSDNFCPKSHSFGQVSLPMKNESNICMSSSFSHSLSSVRQSSVSATFL 67

Query: 65  SSSSTAAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAF 124
           ++S  AAAA+      +++LWR+IHGCNNWEDL+EP LHPLLR+E+IRYGEFVAACYK F
Sbjct: 68  ATSMAAAAAVESSRPKLSNLWREIHGCNNWEDLMEP-LHPLLRQEIIRYGEFVAACYKTF 127

Query: 125 DLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIG 184
           DLDPNSKRYL CK+GKKN+F++VG+ENSGY+V+KYIYATP  I IP +Q S     RWIG
Sbjct: 128 DLDPNSKRYLNCKYGKKNMFRDVGMENSGYDVTKYIYATPD-ITIP-VQAS--GTSRWIG 187

Query: 185 YVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFL 244
           YVAVSSD    RLGRRDI+ITFRGTVT PEWIA LMSSLTPA LD +N RPDVKV SGFL
Sbjct: 188 YVAVSSDKEVKRLGRRDILITFRGTVTNPEWIANLMSSLTPARLDPHNPRPDVKVESGFL 247

Query: 245 TLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAE 304
            LYT +E  +KFG ESCREQLLSE+SRLLNKYK EE+S+T+AGHSMGS+L  LLAYDIAE
Sbjct: 248 NLYTSDESSSKFGPESCREQLLSEISRLLNKYKGEELSVTLAGHSMGSSLANLLAYDIAE 307

Query: 305 LGLNKRT--NKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLF 349
           LGLNKR    K+ +P++VFSFGGP+VGNL FK+RCEELGVK+LRIVNVNDPITKMPGVLF
Sbjct: 308 LGLNKRNYDRKDDIPVTVFSFGGPRVGNLGFKERCEELGVKILRIVNVNDPITKMPGVLF 367

BLAST of Cp4.1LG08g05730 vs. TrEMBL
Match: A0A061ERH8_THECC (Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao GN=TCM_020070 PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.5e-124
Identity = 237/380 (62.37%), Postives = 283/380 (74.47%), Query Frame = 1

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSS---------------ST 60
           MA   L  +   NH  F   I+   HSFGQV LPK++  +SSS               + 
Sbjct: 1   MASKVLMPSINMNHIAFPGDIQRRSHSFGQVCLPKKT-GISSSRQSVIANNSVETPVMAV 60

Query: 61  AAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPN 120
            AAA+   ++ ++  WR+I G NNWE+LVEP LHPLLR+E+IRYGEFVAACYKAFDLDPN
Sbjct: 61  TAAAVSKTSSILSRFWREIQGSNNWENLVEP-LHPLLRKEIIRYGEFVAACYKAFDLDPN 120

Query: 121 SKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYVAVS 180
           S+RYL CK+GKK++ KEVG+ENSGY+V+KYIYATP  +NIP IQ +  +CGRWIGYVAVS
Sbjct: 121 SRRYLNCKYGKKSMLKEVGMENSGYQVTKYIYATPD-VNIP-IQTA--SCGRWIGYVAVS 180

Query: 181 SDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYTC 240
           SD    RLGRRD++ITFRGTVT  EW+A  MSSLTPA LD +N RP+VKV SGFL+LYT 
Sbjct: 181 SDDAVKRLGRRDVLITFRGTVTNHEWVANFMSSLTPARLDPHNPRPEVKVESGFLSLYTS 240

Query: 241 NEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNK 300
           +E  +KFGLESCREQLLSEVSR+LNKYK E++SIT+AGHSMGSAL +L AYDI ELGLNK
Sbjct: 241 DETDSKFGLESCREQLLSEVSRILNKYKGEKLSITVAGHSMGSALALLFAYDITELGLNK 300

Query: 301 RTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS----- 349
             + + +P++VFSFGGP+VGN +FKQRCEELGVKVLRI NVNDPITK+PGVLFN      
Sbjct: 301 TDSDQEIPVTVFSFGGPRVGNSSFKQRCEELGVKVLRIANVNDPITKLPGVLFNENFRVL 360

BLAST of Cp4.1LG08g05730 vs. TrEMBL
Match: M5Y0L8_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa014790mg PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.7e-120
Identity = 223/326 (68.40%), Postives = 256/326 (78.53%), Query Frame = 1

Query: 43  SSTAAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDL 102
           ++T   A     + +   WR+I G NNWE+LVEP LHPLLR+E+IRYG+FV ACYKAF+L
Sbjct: 12  ANTKTTASTRTTSNLAHQWREIQGSNNWENLVEP-LHPLLRQEIIRYGDFVTACYKAFNL 71

Query: 103 DPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYV 162
           DPNSKRYL CK+GKKN+F+EVGLE+ GYEV+KYIYATP  INIP IQN   +CGRW+GYV
Sbjct: 72  DPNSKRYLNCKYGKKNMFREVGLESCGYEVTKYIYATPD-INIP-IQNGE-SCGRWMGYV 131

Query: 163 AVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTL 222
           AVSSD    RLGRRDIVITFRGTVT PEW+A LMSSLTPA LD +N R DVKV SGFL+L
Sbjct: 132 AVSSDDAVKRLGRRDIVITFRGTVTNPEWVANLMSSLTPARLDPHNPRDDVKVESGFLSL 191

Query: 223 YTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELG 282
           YT +E  +KFGL SCREQLLSEVSRLLNKYK EE+SIT+AGHSMGS+L +LL YDIAELG
Sbjct: 192 YTSDESESKFGLGSCREQLLSEVSRLLNKYKGEELSITLAGHSMGSSLALLLGYDIAELG 251

Query: 283 LNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFN--- 342
           LN+  ++  +PI+VFSFGGP+VGN  FKQRCEELGVKVLRIVNVNDPITKMPGV+FN   
Sbjct: 252 LNRENSRRKIPITVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVVFNLEN 311

Query: 343 -----------------SYEHVGVEL 349
                             Y HVGVEL
Sbjct: 312 FRVLGGGESFEFPWSCSCYTHVGVEL 333

BLAST of Cp4.1LG08g05730 vs. TrEMBL
Match: B9GU83_POPTR (Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0002s23240g PE=4 SV=2)

HSP 1 Score: 440.7 bits (1132), Expect = 1.7e-120
Identity = 223/325 (68.62%), Postives = 260/325 (80.00%), Query Frame = 1

Query: 41  SSSSTAAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAF 100
           +SSST+++++ H       +WR++ GCNNW+ LVEP LHP LR+E+IRYGEFV ACY+AF
Sbjct: 8   TSSSTSSSSLAH-------MWREVQGCNNWDGLVEP-LHPFLRQEIIRYGEFVTACYEAF 67

Query: 101 DLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIG 160
           DL+P SKRYLTCK+GKKNLF+EVG+ NS YEV+KYIYATP  +NIP IQN P +CGRWIG
Sbjct: 68  DLNPKSKRYLTCKYGKKNLFREVGMGNSDYEVTKYIYATPD-VNIP-IQNEP-SCGRWIG 127

Query: 161 YVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFL 220
           YVAVSSD    RLGRRDIVITFRGTVT PEWI+ LMSSLTPA LD  N RP+VKV SGFL
Sbjct: 128 YVAVSSDDAVRRLGRRDIVITFRGTVTNPEWISNLMSSLTPARLDPNNPRPEVKVESGFL 187

Query: 221 TLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAE 280
           +LYT NE   KFGL SCREQLLSEVSRLLN+YK EE+SI++AGHSMGS+L +LLAYDIAE
Sbjct: 188 SLYTSNESDNKFGLGSCREQLLSEVSRLLNRYKGEELSISLAGHSMGSSLALLLAYDIAE 247

Query: 281 LGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS 340
           LGLN+   K  VP++VFSFGGP+VGN +FK+RCEELGVKVLRI NVNDPITK+PGVL N 
Sbjct: 248 LGLNRLDPKLDVPVTVFSFGGPRVGNSSFKERCEELGVKVLRIANVNDPITKLPGVLLNE 307

Query: 341 -----------------YEHVGVEL 349
                            YEHVGVE+
Sbjct: 308 NLRVFGGRYEFPWSCSCYEHVGVEI 321

BLAST of Cp4.1LG08g05730 vs. TAIR10
Match: AT2G31690.1 (AT2G31690.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 405.6 bits (1041), Expect = 3.1e-113
Identity = 215/390 (55.13%), Postives = 259/390 (66.41%), Query Frame = 1

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSSSTAAAAMVHKNAGMTS- 60
           M H     N  P    F     S P S+      K +++ SSSS  A  +++     +S 
Sbjct: 9   MKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSSSSSSLLAPVILNSPVASSSP 68

Query: 61  ----------------------LWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACY 120
                                 +WR+I GCNNW+DL+EP L+PLL++E+ RYG  V+ CY
Sbjct: 69  PPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDLIEP-LNPLLQQEITRYGNLVSTCY 128

Query: 121 KAFDLDPNSKRYLTCKFGKKNLFKEVGLEN-SGYEVSKYIYATPPF-INIPPIQNSPPAC 180
           KAFDLDPNSKRYL CK+GK+ L KE  ++    Y+V+KYIYATP   INI PIQN     
Sbjct: 129 KAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININISPIQNEMNRR 188

Query: 181 GRWIGYVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKV 240
            RW+GYVA SSD    RLGRRDIV+TFRGTVT PEW+A  MSSLTPA    +N R DVKV
Sbjct: 189 ARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHPHNPRLDVKV 248

Query: 241 LSGFLTLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLA 300
            SGFL+LYT +E  +KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSMGS+L  LLA
Sbjct: 249 ESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSMGSSLAQLLA 308

Query: 301 YDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPG 349
           YDIAELGLN+R  K  +P++VFSF GP+VGNL FK+RCEELGVKVLRI NVNDP+TK+PG
Sbjct: 309 YDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITNVNDPVTKLPG 368

BLAST of Cp4.1LG08g05730 vs. TAIR10
Match: AT1G05800.1 (AT1G05800.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 405.2 bits (1040), Expect = 4.0e-113
Identity = 210/349 (60.17%), Postives = 258/349 (73.93%), Query Frame = 1

Query: 18  SDTIRSNPHSFGQVSLPKRSDNVSSSSTAAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPN 77
           S +  S P S   +SLP  S + +   + A A+      ++ +WR+I G NNWE+L+EP 
Sbjct: 44  SSSTMSPPISSSPLSLPSSSSSQAIPPSRAPAVT---LPLSRVWREIQGSNNWENLIEP- 103

Query: 78  LHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLEN-SGYEVSKYI 137
           L P+L++E+ RYG  ++A YK FDL+PNSKRYL+CK+GKKNL KE G+ +  GY+V+KYI
Sbjct: 104 LSPILQQEITRYGNLLSASYKGFDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYI 163

Query: 138 YATPPFINIPPIQNSPPACGRWIGYVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILM 197
           YATP  IN+ PI+N P    RWIGYVAVSSD    RLGRRDI++TFRGTVT  EW+A L 
Sbjct: 164 YATPD-INLNPIKNEPNRA-RWIGYVAVSSDESVKRLGRRDILVTFRGTVTNHEWLANLK 223

Query: 198 SSLTPAHLDCYNYRPDVKVLSGFLTLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEE 257
           SSLTPA LD +N RPDVKV SGFL LYT  E  +KFGLESCREQLLSE+SRL+NK+K EE
Sbjct: 224 SSLTPARLDPHNPRPDVKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKHKGEE 283

Query: 258 VSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEEL 317
           +SIT+AGHSMGS+L  LLAYDIAELG+N+R +++ VP++VFSF GP+VGNL FK+RCEEL
Sbjct: 284 ISITLAGHSMGSSLAQLLAYDIAELGMNQRRDEKPVPVTVFSFAGPRVGNLGFKKRCEEL 343

Query: 318 GVKVLRIVNVNDPITKMPGVLFNS-----------------YEHVGVEL 349
           GVKVLRI NVNDPITK+PG LFN                  Y HVGVEL
Sbjct: 344 GVKVLRITNVNDPITKLPGFLFNENFRSLGGVYELPWSCSCYTHVGVEL 386

BLAST of Cp4.1LG08g05730 vs. TAIR10
Match: AT1G51440.1 (AT1G51440.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 268.1 bits (684), Expect = 7.7e-72
Identity = 148/326 (45.40%), Postives = 197/326 (60.43%), Query Frame = 1

Query: 53  KNAGMTSLWRQIHGCNNWEDLVEP-NLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLT 112
           K   +  +WR++ GCNNWE  ++P N H  LRRE+IRYGEF  ACY +FD DP+SK   +
Sbjct: 83  KEVSLREIWREVQGCNNWEGQLDPMNNH--LRREIIRYGEFAQACYDSFDFDPHSKYCGS 142

Query: 113 CKFGKKNLFKEVGLE-NSGYEVSKYIYAT-----PPFINIPPIQNSPPACGRWIGYVAVS 172
           CK+   + F  + L  + GY +++Y+YAT     P F     + +       W+G+VAV+
Sbjct: 143 CKYHPSDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVA 202

Query: 173 SDYMS-TRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYT 232
           +D    +RLGRRDIVI +RGTVT  EWI  L   L  A+   +   P +K+  GF  LYT
Sbjct: 203 TDEEEVSRLGRRDIVIAWRGTVTYLEWIYDLKDILCSAN---FGDDPSIKIELGFHDLYT 262

Query: 233 CNEKCTKFGLESCREQLLSEVSRLLNKYKNEE----VSITMAGHSMGSALGVLLAYDIAE 292
             E   KF   S REQ+L+EV RL+  Y  EE     SIT+ GHS+G++L ++ AYDIAE
Sbjct: 263 KKEDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAE 322

Query: 293 LGLNK-RTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFN 349
           L LN    N   +PI+VFSF GP+VGNL FK+RC+ELGVKVLR+VNV+D +  +PG+  N
Sbjct: 323 LNLNHVPENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTN 382

BLAST of Cp4.1LG08g05730 vs. TAIR10
Match: AT1G06800.1 (AT1G06800.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 266.2 bits (679), Expect = 2.9e-71
Identity = 138/291 (47.42%), Postives = 192/291 (65.98%), Query Frame = 1

Query: 61  WRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLF 120
           WR+I G ++W  L++P + P+LR E+IRYGE   ACY AFD DP S+   +C+F +++LF
Sbjct: 89  WRKIQGEDDWAGLMDP-MDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLF 148

Query: 121 KEVGLENSGYEVSKYIYATPPFINIPPIQNSP------PACGRWIGYVAVSSDYMSTR-- 180
             +G+ +SGYEV++Y+YAT   IN+P   +             W+GYVAVS D  +TR  
Sbjct: 149 DSLGIIDSGYEVARYLYATSN-INLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCR 208

Query: 181 LGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYR-PDVKVLSGFLTLYTCNEKCTK 240
           LGRRDI I +RGTVT  EWIA L   L P   + +    P VK  SGFL LYT  +    
Sbjct: 209 LGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCN 268

Query: 241 FGLESCREQLLSEVSRLLNKY---KNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTN 300
           F   S REQ+L+EV RL+ +Y   + EE+SIT+ GHS+G AL VL AYD+AE+G+N+   
Sbjct: 269 FSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRK 328

Query: 301 KEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFN 340
            + +P++ F++GGP+VGN+ FK+R E+LGVKVLR+VN +D + K PG+  N
Sbjct: 329 GKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLN 377

BLAST of Cp4.1LG08g05730 vs. TAIR10
Match: AT2G30550.2 (AT2G30550.2 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 261.2 bits (666), Expect = 9.4e-70
Identity = 144/317 (45.43%), Postives = 196/317 (61.83%), Query Frame = 1

Query: 61  WRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLF 120
           WR+I G ++W  L++P + P+LR E+IRYGE   ACY AFD DP SK   T +F +   F
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 121 KEVGLENSGYEVSKYIYATPPFINIPPIQNSP------PACGRWIGYVAVSSDYMS-TRL 180
             +G+ +SGYEV++Y+YAT   IN+P   +             W+GYVAVS D  S  RL
Sbjct: 165 DSLGMIDSGYEVARYLYATSN-INLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 181 GRRDIVITFRGTVTIPEWIAILMSSLTPA---HLDCYNYRPDVKVLSGFLTLYTCNEKCT 240
           GRRDI I +RGTVT  EWIA L   L P     + C +  P VKV SGFL LYT  +   
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPD--PAVKVESGFLDLYTDKDTTC 284

Query: 241 KFGLESCREQLLSEVSRLLNKYKNEE---VSITMAGHSMGSALGVLLAYDIAELGLNKRT 300
           KF   S REQ+L+EV RL+ ++ +++   +SIT+ GHS+G AL +L AYDIAE+ LN+  
Sbjct: 285 KFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSK 344

Query: 301 NKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS------- 349
             + +P++V ++GGP+VGN+ F++R EELGVKV+R+VNV+D + K PG+  N        
Sbjct: 345 KGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALM 404

BLAST of Cp4.1LG08g05730 vs. NCBI nr
Match: gi|659077199|ref|XP_008439078.1| (PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis melo])

HSP 1 Score: 563.9 bits (1452), Expect = 1.9e-157
Identity = 291/405 (71.85%), Postives = 313/405 (77.28%), Query Frame = 1

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSD-NVSSSST-------------- 60
           MA TTLKLNSIPNH TF +T R   HSFGQVS+P++SD NVSSSS+              
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  --------------------------AAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLH 120
                                     AAAAMV K A +  LWRQIHGCN+WEDLVEP LH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYAT 180
           PLLRRE+IRYGEFV ACYKAFDLDPNSKRYLTCKFGKK+L KEVGLE+SGYEV+KYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPFINIPPIQNSPPACGRWIGYVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSL 240
           PP INIPPIQNSPP+CGRWIGYVAVSSD  S RLGRRDI+ITFRGTVT PEWIA LMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPAHLDCYNYRPDVKVLSGFLTLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSI 300
           TPA LD +N+RPDVKV SGFLTLYT  E   KFGLESCREQLLSEVSR+LNKYK EEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVK 349
           TMAGHSMGSAL +LLAYDIAELGLN+RTN E VP+SVFSFGGP+VGN  FK+RC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

BLAST of Cp4.1LG08g05730 vs. NCBI nr
Match: gi|778679286|ref|XP_004148168.2| (PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 563.5 bits (1451), Expect = 2.5e-157
Identity = 291/402 (72.39%), Postives = 311/402 (77.36%), Query Frame = 1

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKR-SDNVSSSST-------------- 60
           MA TTLKLNSIPNH TF +T R   HSFGQVS+P++   NVSSSS+              
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  -----------------------AAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLL 120
                                  AAAAMV K A +  LWRQIHGCN+WEDLVEP LHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREVIRYGEFVAACYKAFDLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPF 180
           RRE+IRYGEFV ACYKAFDLDPNSKRYLTCKFGKK+L KEVGLE+SGYEV+KYIYATPP 
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPACGRWIGYVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPA 240
           INIPPIQNSPP+CGRWIGYVAVSSD  S RLGRRDI+ITFRGTVT PEWIA LMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 HLDCYNYRPDVKVLSGFLTLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMA 300
            LD +N+RPDVKV SGFLTLYT  E   KFGLESCREQLLSEVSRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALGVLLAYDIAELGLNKRTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLR 349
           GHSMGSAL +LLAYDIAELGLN+RTN E VP+SVFSFGGP+VGN  FK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

BLAST of Cp4.1LG08g05730 vs. NCBI nr
Match: gi|703132772|ref|XP_010105205.1| (Galactolipase DONGLE [Morus notabilis])

HSP 1 Score: 467.2 bits (1201), Expect = 2.4e-128
Identity = 244/387 (63.05%), Postives = 285/387 (73.64%), Query Frame = 1

Query: 5   TLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDN------------------------V 64
           TL   +  +H  FSD      HSFGQVSLP ++++                        +
Sbjct: 8   TLNTKTNNHHIIFSDNFCPKSHSFGQVSLPMKNESNICMSSSFSHSLSSVRQSSVSATFL 67

Query: 65  SSSSTAAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAF 124
           ++S  AAAA+      +++LWR+IHGCNNWEDL+EP LHPLLR+E+IRYGEFVAACYK F
Sbjct: 68  ATSMAAAAAVESSRPKLSNLWREIHGCNNWEDLMEP-LHPLLRQEIIRYGEFVAACYKTF 127

Query: 125 DLDPNSKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIG 184
           DLDPNSKRYL CK+GKKN+F++VG+ENSGY+V+KYIYATP  I IP +Q S     RWIG
Sbjct: 128 DLDPNSKRYLNCKYGKKNMFRDVGMENSGYDVTKYIYATPD-ITIP-VQAS--GTSRWIG 187

Query: 185 YVAVSSDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFL 244
           YVAVSSD    RLGRRDI+ITFRGTVT PEWIA LMSSLTPA LD +N RPDVKV SGFL
Sbjct: 188 YVAVSSDKEVKRLGRRDILITFRGTVTNPEWIANLMSSLTPARLDPHNPRPDVKVESGFL 247

Query: 245 TLYTCNEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAE 304
            LYT +E  +KFG ESCREQLLSE+SRLLNKYK EE+S+T+AGHSMGS+L  LLAYDIAE
Sbjct: 248 NLYTSDESSSKFGPESCREQLLSEISRLLNKYKGEELSVTLAGHSMGSSLANLLAYDIAE 307

Query: 305 LGLNKRT--NKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLF 349
           LGLNKR    K+ +P++VFSFGGP+VGNL FK+RCEELGVK+LRIVNVNDPITKMPGVLF
Sbjct: 308 LGLNKRNYDRKDDIPVTVFSFGGPRVGNLGFKERCEELGVKILRIVNVNDPITKMPGVLF 367

BLAST of Cp4.1LG08g05730 vs. NCBI nr
Match: gi|590655475|ref|XP_007033998.1| (Alpha/beta-Hydrolases superfamily protein [Theobroma cacao])

HSP 1 Score: 454.1 bits (1167), Expect = 2.1e-124
Identity = 237/380 (62.37%), Postives = 283/380 (74.47%), Query Frame = 1

Query: 1   MAHTTLKLNSIPNHFTFSDTIRSNPHSFGQVSLPKRSDNVSSS---------------ST 60
           MA   L  +   NH  F   I+   HSFGQV LPK++  +SSS               + 
Sbjct: 1   MASKVLMPSINMNHIAFPGDIQRRSHSFGQVCLPKKT-GISSSRQSVIANNSVETPVMAV 60

Query: 61  AAAAMVHKNAGMTSLWRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPN 120
            AAA+   ++ ++  WR+I G NNWE+LVEP LHPLLR+E+IRYGEFVAACYKAFDLDPN
Sbjct: 61  TAAAVSKTSSILSRFWREIQGSNNWENLVEP-LHPLLRKEIIRYGEFVAACYKAFDLDPN 120

Query: 121 SKRYLTCKFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYVAVS 180
           S+RYL CK+GKK++ KEVG+ENSGY+V+KYIYATP  +NIP IQ +  +CGRWIGYVAVS
Sbjct: 121 SRRYLNCKYGKKSMLKEVGMENSGYQVTKYIYATPD-VNIP-IQTA--SCGRWIGYVAVS 180

Query: 181 SDYMSTRLGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYTC 240
           SD    RLGRRD++ITFRGTVT  EW+A  MSSLTPA LD +N RP+VKV SGFL+LYT 
Sbjct: 181 SDDAVKRLGRRDVLITFRGTVTNHEWVANFMSSLTPARLDPHNPRPEVKVESGFLSLYTS 240

Query: 241 NEKCTKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNK 300
           +E  +KFGLESCREQLLSEVSR+LNKYK E++SIT+AGHSMGSAL +L AYDI ELGLNK
Sbjct: 241 DETDSKFGLESCREQLLSEVSRILNKYKGEKLSITVAGHSMGSALALLFAYDITELGLNK 300

Query: 301 RTNKEAVPISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFNS----- 349
             + + +P++VFSFGGP+VGN +FKQRCEELGVKVLRI NVNDPITK+PGVLFN      
Sbjct: 301 TDSDQEIPVTVFSFGGPRVGNSSFKQRCEELGVKVLRIANVNDPITKLPGVLFNENFRVL 360

BLAST of Cp4.1LG08g05730 vs. NCBI nr
Match: gi|645231997|ref|XP_008222660.1| (PREDICTED: galactolipase DONGLE, chloroplastic [Prunus mume])

HSP 1 Score: 453.0 bits (1164), Expect = 4.8e-124
Identity = 240/376 (63.83%), Postives = 274/376 (72.87%), Query Frame = 1

Query: 14  HFTFSDTIRS-NPHSFGQVSLPKRSDNVSSSSTAAAAMVHKNAGMTSL------------ 73
           H TF   IR  +  SFGQV LPKR++  +   T  AA+       T+             
Sbjct: 15  HVTFPKNIRPRSSDSFGQVCLPKRTETAAGQQTIVAAVATVEVAATTSTRANTKTTASTR 74

Query: 74  --------WRQIHGCNNWEDLVEPNLHPLLRREVIRYGEFVAACYKAFDLDPNSKRYLTC 133
                   WR+I G NNWE+LVEP LHPLLR+E+IRYG+FV ACYKAFDLDPNSKRYL C
Sbjct: 75  ITSNLANQWREIQGSNNWENLVEP-LHPLLRQEIIRYGDFVTACYKAFDLDPNSKRYLNC 134

Query: 134 KFGKKNLFKEVGLENSGYEVSKYIYATPPFINIPPIQNSPPACGRWIGYVAVSSDYMSTR 193
           K+GKKN+F+EVGLE+ GYEV+KYIYATP  INIP IQN   +CGRW+GYVAVSSD    R
Sbjct: 135 KYGKKNMFREVGLESCGYEVTKYIYATPD-INIP-IQNGE-SCGRWMGYVAVSSDDAVKR 194

Query: 194 LGRRDIVITFRGTVTIPEWIAILMSSLTPAHLDCYNYRPDVKVLSGFLTLYTCNEKCTKF 253
           LGRRDIVITFRGTVT PEW+A LMSSLTPA LD +N R DVKV SGFL+LYT +E  +KF
Sbjct: 195 LGRRDIVITFRGTVTNPEWVANLMSSLTPARLDPHNPRDDVKVESGFLSLYTSDESESKF 254

Query: 254 GLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALGVLLAYDIAELGLNKRTNKEAV 313
           GL SCREQLLSEVSRLLNKYK EE+SIT+AGHSMGS+L +LL YDIAELGLN+  ++  +
Sbjct: 255 GLGSCREQLLSEVSRLLNKYKGEELSITLAGHSMGSSLALLLGYDIAELGLNRENSRRKI 314

Query: 314 PISVFSFGGPKVGNLNFKQRCEELGVKVLRIVNVNDPITKMPGVLFN------------- 349
           PI+VFSFGGP+VGN  FKQRCEELGVKVLRIVNVNDPITKMPGV+FN             
Sbjct: 315 PITVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVVFNLENFRVLGGGGSF 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PLA13_ARATH5.5e-11255.13Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana GN=At2g31690 PE=... [more]
PLA12_ARATH7.1e-11260.17Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana GN=DGL PE=1 SV=1[more]
EG1_ORYSI2.6e-9857.79Phospholipase A1 EG1, chloroplastic OS=Oryza sativa subsp. indica GN=EG1 PE=3 SV... [more]
EG1_ORYSJ2.6e-9857.79Phospholipase A1 EG1, chloroplastic OS=Oryza sativa subsp. japonica GN=EG1 PE=1 ... [more]
PLA17_ARATH1.4e-7045.40Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana GN=At1g51440 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0L8A7_CUCSA1.7e-15772.39Uncharacterized protein OS=Cucumis sativus GN=Csa_3G174570 PE=4 SV=1[more]
W9SFC4_9ROSA1.7e-12863.05Galactolipase DONGLE OS=Morus notabilis GN=L484_011039 PE=4 SV=1[more]
A0A061ERH8_THECC1.5e-12462.37Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao GN=TCM_020070 PE=4 ... [more]
M5Y0L8_PRUPE1.7e-12068.40Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa014790mg PE=4 S... [more]
B9GU83_POPTR1.7e-12068.62Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0002s23240g P... [more]
Match NameE-valueIdentityDescription
AT2G31690.13.1e-11355.13 alpha/beta-Hydrolases superfamily protein[more]
AT1G05800.14.0e-11360.17 alpha/beta-Hydrolases superfamily protein[more]
AT1G51440.17.7e-7245.40 alpha/beta-Hydrolases superfamily protein[more]
AT1G06800.12.9e-7147.42 alpha/beta-Hydrolases superfamily protein[more]
AT2G30550.29.4e-7045.43 alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659077199|ref|XP_008439078.1|1.9e-15771.85PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis melo][more]
gi|778679286|ref|XP_004148168.2|2.5e-15772.39PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis sativus][more]
gi|703132772|ref|XP_010105205.1|2.4e-12863.05Galactolipase DONGLE [Morus notabilis][more]
gi|590655475|ref|XP_007033998.1|2.1e-12462.37Alpha/beta-Hydrolases superfamily protein [Theobroma cacao][more]
gi|645231997|ref|XP_008222660.1|4.8e-12463.83PREDICTED: galactolipase DONGLE, chloroplastic [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR002921Fungal_lipase-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0009395 phospholipid catabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0052689 carboxylic ester hydrolase activity
molecular_function GO:0004620 phospholipase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g05730.1Cp4.1LG08g05730.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 179..336
score: 2.4
NoneNo IPR availableunknownCoilCoilcoord: 238..258
scor
NoneNo IPR availablePANTHERPTHR31403FAMILY NOT NAMEDcoord: 33..348
score: 6.8E
NoneNo IPR availablePANTHERPTHR31403:SF4GALACTOLIPASE DONGLE, CHLOROPLASTIC-RELATEDcoord: 33..348
score: 6.8E