Cp4.1LG08g04410 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG08g04410
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionProlyl 4-hydroxylase subunit alpha-2
LocationCp4.1LG08 : 1499807 .. 1502953 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGGACTTAGAAAGTTTGGCAGTTGAAGCAGCTAAGGGAACTAGAAATCTGAAAATTTTCAGAGGAAAGAAACCTTTTATTATTTTATTTTCATCGCTCTCTCTTTCTCCACTGATCCGATTCAGTCAATGGCGAAATTTTGTAGTTGCAATCTGCTGTTTATCCTCTCGATATCGATCTCGTTGCTTCTCCGGCGAGCTTCAAGCTCTTATGCAGGTTCCGCTAGCTCAATCGTCAATCCTGCAAAAGTCAAACAGATTTCATGGAGTCCCCGGTACTAAATCGTTCACCATTCTGCTCGTAGTCTTCTTGTTTTGGTATCTAGATTTTGTTAATCGCATTCTTCGTTTAGATTTATTCACGGTCTGATGTTTCCGTTTCCGTTTCAATTTGAATGATTATGCAGAGCTTTCGTGTATGAAGGTTTTCTCACGGACTTGGAATGCGATCATCTCATCTCGCTTGTGAGTTGAAATAGTATCGACTCATTTTTTTTTTTACGCGATTTTTGTTTTGGTTAATTTTTGTTTTTTGTTTAAATTAGGCTAAAGCGGAGTTGAAGAGATCTGCTGTTGCGGATCATTTGTCTGGAGAGAGCAAGGTCAGCGAGGTCCGAACTAGCTCTGGGGCATTTATTCATAAAGCCAAGGTTTGTGCGGCGTTTCGTTCTTATAGTTTTTAAGTTTCCACTATAGAAGCATACTGATCTTAAATCACCTTCAAACCTAAATCCTACACTTTTATTTCGATATGTATTGGTTTAATAAACATTTAAGATTCTCCAGAAATTCAGATATGAGTTTGGTTCATGAGATCCATCGGTTAAATATTGGTTTGACTTATGAAGTTTATCTTAATATGAAATGAGTGAATGAATGCCATTGCAGTTATTTAAACCAGATTTTCTATTCTGATATTGTTATAAGTCACCATTTGAAAAGAAAAGGAAAATTTTTGAACCGTTGATTGAAGCAATATTCAACAATCGACATTCATTTAGAAGGGGCGGCTGTTATTACTTCTTCATTCTACCTTGGTTTGTCAAATTTTTTAAGAATGTTATTACTTCTTCATTCTGAACTAGTTTGAATGATTGGTATCTTGGTCTGTCAAATTTTTTATGAATGTTATTACTTCTTCATTCTGAACTAGTCTGGATGATTGGTATCTTGGTTTGTCAAATTTTTTTAAGAATGTTATTACTTCTTCATTTCGAATTTTTCTGTCTTACTGACTATTGAAGCAGGAAACATTAAAGGTCACCCTTAATTCTTCTCAGTATACAGATAGGGTTTAGGCACAAATTTCTAACCTGATAACCGTTCTCTATCTCCCCTCCGTGAACTTATTGTCTGTATATCATGTAGGATCCGATCGTTTCTGGTATTGAAGACAAAATTGCAGCATGGACATTCCTGCCAAAAGGTAAGCCATTAGCAGAAGGCGCAAATTTCTGGTTAAATTCCATAGTCTTAAGCAACTTATGTTAGATTATAAATTGCTTTCCGTTATCAATAGCAACTCTTGATTTTAACAGGATAGAGGCATTTGCAGTTGAATTTAAATTTTGGGTTCTCAAGTTCTATCCATTCTTATGACTGAAACCATAAAGAGAAATATTGGACTAATCGTATTAAATCTCTAAAGTGAACAAACCAGATAACGAAAAGTTATCTCATTTTCTGTATGCAACTAGCACAGTTCTTTCACCAATATTTGGTTCTGATTTCCGTATAAATTAGTGAACTCCAGAAGACATGATCCCCTGTCATAATTATATAATACATAATCAAGTGATAGAGTCTAAAAAGAATTTTGCAGCTGGGCAGAAACACAGTGTTTCGACTAAGCCCTTTCATTTTTTTGGTGAAAAAGGAAAAGGAAAACTATTGAGTTTGAAACTAAATTTATATAAGATGTGGAAATTTTGACTCATGCATTGATAGCTTGTTTATGCATTGGTTTTACTGGTAAATTGAAACTTGAATGTGCTTGATTTCAGAAAATGGAGAAGACATTCAAGTGTTGAGATATGAATATGGGCAGAAGTATGATGTCCATTACGATTACTTTACTGACAAGGTTAATATTGCCCGAGGTGGACACCGAATGGCAACTGTTCTTATGTATCTTTCCAATGTAGAAAAAGGTGGTGAAACTGTGTTTCCTAATGCCGAGGTTTGTCCTGGTGTTTCCCTTTAATCCAATTGATTGCAACCCTTTTTTCTGTACTTCGGCCATAATCAAGAGCTTTGGTTTTTCTTGGTACAATATTCTTTGCAAATATTCTTCTCTCGAGTTGTCGTACTTAAGGAAGATATATCTGTCTCTACTTATTGACTTACTTCATCAAATCCGCTGTATGAACTGATAATTTATCAACATTTTTCTTTTCAATCAAAATATCAATATTGAATATGTTTGTTCATTCATGTGAGTTCAGGAATCTCAAAGACGGCAGGCTTCTGAAACAAATGAAGATCTCTCAGACTGTGCAAAGAAAGGGATAGCAGGTGAGTGTTTTAAGTCAGTTTTCTAACATCTATCCTATTTATGAAGTCAGTAGAAAGCGATAACTCGTCGAGATTTCTCTCTTTAATCTCCACCTTTAAAATATTCAGTGAAACCACGGAAGGGTGATGCTCTTCTCTTCTTCAGTCTTCATCCAAATGCTGTTCCAGACACAAAAAGTCTGCATGGAGGTTGCCCTGTGATTGAAGGAGAGAAATGGTCAGCAACAAAGTGGATTCATGTCGATTCTTTCGACACGATCGTGAGTGATCATACGAGTTGCGTTGATAACAATGCAAGTTGTGAGAGATGGGCTGAACTCGGTGAGTGCACGAATAATCCGGAGTATATGGTGGGATCTCCTGAGCTTCCTGGCTATTGCAGGAAAAGTTGCAAGGTTTGTTGATAAACTTGCTCGGTTCTTTTATGTGCATTCCTTCCATTTCTCAGTGTTTTGCAGAGCTTGTCCAAAAACACAAATGGTTGTTTTCCTGATAGCATTTTCATTGCAATGTTTTGTGAGAGTTAGCATATGTATTGATACCTCAATCATGTAACATTATTTGAAAATAATTTAGTTTTGGCAAGTTATTCGGATTTTAAGAGTTTTTTTTAATTGAAAAACGTTCCCTTTTC

mRNA sequence

TGGGACTTAGAAAGTTTGGCAGTTGAAGCAGCTAAGGGAACTAGAAATCTGAAAATTTTCAGAGGAAAGAAACCTTTTATTATTTTATTTTCATCGCTCTCTCTTTCTCCACTGATCCGATTCAGTCAATGGCGAAATTTTGTAGTTGCAATCTGCTGTTTATCCTCTCGATATCGATCTCGTTGCTTCTCCGGCGAGCTTCAAGCTCTTATGCAGGTTCCGCTAGCTCAATCGTCAATCCTGCAAAAGTCAAACAGATTTCATGGAGTCCCCGAGCTTTCGTGTATGAAGGTTTTCTCACGGACTTGGAATGCGATCATCTCATCTCGCTTGCTAAAGCGGAGTTGAAGAGATCTGCTGTTGCGGATCATTTGTCTGGAGAGAGCAAGGTCAGCGAGGTCCGAACTAGCTCTGGGGCATTTATTCATAAAGCCAAGGATCCGATCGTTTCTGGTATTGAAGACAAAATTGCAGCATGGACATTCCTGCCAAAAGAAAATGGAGAAGACATTCAAGTGTTGAGATATGAATATGGGCAGAAGTATGATGTCCATTACGATTACTTTACTGACAAGGTTAATATTGCCCGAGGTGGACACCGAATGGCAACTGTTCTTATGTATCTTTCCAATGTAGAAAAAGGTGGTGAAACTGTGTTTCCTAATGCCGAGGAATCTCAAAGACGGCAGGCTTCTGAAACAAATGAAGATCTCTCAGACTGTGCAAAGAAAGGGATAGCAGTGAAACCACGGAAGGGTGATGCTCTTCTCTTCTTCAGTCTTCATCCAAATGCTGTTCCAGACACAAAAAGTCTGCATGGAGGTTGCCCTGTGATTGAAGGAGAGAAATGGTCAGCAACAAAGTGGATTCATGTCGATTCTTTCGACACGATCGTGAGTGATCATACGAGTTGCGTTGATAACAATGCAAGTTGTGAGAGATGGGCTGAACTCGGTGAGTGCACGAATAATCCGGAGTATATGGTGGGATCTCCTGAGCTTCCTGGCTATTGCAGGAAAAGTTGCAAGGTTTGTTGATAAACTTGCTCGGTTCTTTTATGTGCATTCCTTCCATTTCTCAGTGTTTTGCAGAGCTTGTCCAAAAACACAAATGGTTGTTTTCCTGATAGCATTTTCATTGCAATGTTTTGTGAGAGTTAGCATATGTATTGATACCTCAATCATGTAACATTATTTGAAAATAATTTAGTTTTGGCAAGTTATTCGGATTTTAAGAGTTTTTTTTAATTGAAAAACGTTCCCTTTTC

Coding sequence (CDS)

ATGGCGAAATTTTGTAGTTGCAATCTGCTGTTTATCCTCTCGATATCGATCTCGTTGCTTCTCCGGCGAGCTTCAAGCTCTTATGCAGGTTCCGCTAGCTCAATCGTCAATCCTGCAAAAGTCAAACAGATTTCATGGAGTCCCCGAGCTTTCGTGTATGAAGGTTTTCTCACGGACTTGGAATGCGATCATCTCATCTCGCTTGCTAAAGCGGAGTTGAAGAGATCTGCTGTTGCGGATCATTTGTCTGGAGAGAGCAAGGTCAGCGAGGTCCGAACTAGCTCTGGGGCATTTATTCATAAAGCCAAGGATCCGATCGTTTCTGGTATTGAAGACAAAATTGCAGCATGGACATTCCTGCCAAAAGAAAATGGAGAAGACATTCAAGTGTTGAGATATGAATATGGGCAGAAGTATGATGTCCATTACGATTACTTTACTGACAAGGTTAATATTGCCCGAGGTGGACACCGAATGGCAACTGTTCTTATGTATCTTTCCAATGTAGAAAAAGGTGGTGAAACTGTGTTTCCTAATGCCGAGGAATCTCAAAGACGGCAGGCTTCTGAAACAAATGAAGATCTCTCAGACTGTGCAAAGAAAGGGATAGCAGTGAAACCACGGAAGGGTGATGCTCTTCTCTTCTTCAGTCTTCATCCAAATGCTGTTCCAGACACAAAAAGTCTGCATGGAGGTTGCCCTGTGATTGAAGGAGAGAAATGGTCAGCAACAAAGTGGATTCATGTCGATTCTTTCGACACGATCGTGAGTGATCATACGAGTTGCGTTGATAACAATGCAAGTTGTGAGAGATGGGCTGAACTCGGTGAGTGCACGAATAATCCGGAGTATATGGTGGGATCTCCTGAGCTTCCTGGCTATTGCAGGAAAAGTTGCAAGGTTTGTTGA

Protein sequence

MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC
BLAST of Cp4.1LG08g04410 vs. Swiss-Prot
Match: P4H4_ARATH (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana GN=P4H4 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 4.3e-129
Identity = 223/293 (76.11%), Postives = 248/293 (84.64%), Query Frame = 1

Query: 10  LFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLA 69
           L I   +I  +L ++S+S   S+S  VNP+KVKQ+S  PRAFVYEGFLT+LECDH++SLA
Sbjct: 6   LLISFFAIFSVLLQSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLA 65

Query: 70  KAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQ 129
           KA LKRSAVAD+ SGESK SEVRTSSG FI K KDPIVSGIEDKI+ WTFLPKENGEDIQ
Sbjct: 66  KASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQ 125

Query: 130 VLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQAS 189
           VLRYE+GQKYD H+DYF DKVNI RGGHRMAT+LMYLSNV KGGETVFP+AE   RR  S
Sbjct: 126 VLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLS 185

Query: 190 ETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHV 249
           E  EDLSDCAK+GIAVKPRKGDALLFF+LHP+A+PD  SLHGGCPVIEGEKWSATKWIHV
Sbjct: 186 ENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV 245

Query: 250 DSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           DSFD IV+   +C D N SCERWA LGECT NPEYMVG+ ELPGYCR+SCK C
Sbjct: 246 DSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

BLAST of Cp4.1LG08g04410 vs. Swiss-Prot
Match: P4H2_ARATH (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana GN=P4H2 PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 3.7e-125
Identity = 213/291 (73.20%), Postives = 249/291 (85.57%), Query Frame = 1

Query: 12  ILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAKA 71
           +L ++I L+L ++S+    S SSI+NP+KVKQ+S  PRAFVYEGFLTDLECDHLISLAK 
Sbjct: 9   LLFVAILLVLLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKE 68

Query: 72  ELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVL 131
            L+RSAVAD+ +GES+VS+VRTSSG FI K KDPIVSGIEDK++ WTFLPKENGED+QVL
Sbjct: 69  NLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVL 128

Query: 132 RYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQASET 191
           RYE+GQKYD H+DYF DKVNIARGGHR+ATVL+YLSNV KGGETVFP+A+E  RR  SE 
Sbjct: 129 RYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSEN 188

Query: 192 NEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHVDS 251
            +DLSDCAKKGIAVKP+KG+ALLFF+L  +A+PD  SLHGGCPVIEGEKWSATKWIHVDS
Sbjct: 189 KDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDS 248

Query: 252 FDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           FD I++   +C D N SCERWA LGEC  NPEYMVG+PE+PG CR+SCK C
Sbjct: 249 FDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

BLAST of Cp4.1LG08g04410 vs. Swiss-Prot
Match: P4H7_ARATH (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana GN=P4H7 PE=2 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 6.0e-99
Identity = 179/307 (58.31%), Postives = 227/307 (73.94%), Query Frame = 1

Query: 5   CSCNLLFILSISISLLLRRASSSYAGS-------ASSI-VNPAKVKQISWSPRAFVYEGF 64
           C    L ++S + +  L R+S++  GS       ASS   +P +V Q+SW+PR F+YEGF
Sbjct: 12  CFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRVFLYEGF 71

Query: 65  LTDLECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAA 124
           L+D ECDH I LAK +L++S VAD+ SGES  SEVRTSSG F+ K +D IVS +E K+AA
Sbjct: 72  LSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQDDIVSNVEAKLAA 131

Query: 125 WTFLPKENGEDIQVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETV 184
           WTFLP+ENGE +Q+L YE GQKY+ H+DYF D+ N+  GGHR+ATVLMYLSNVEKGGETV
Sbjct: 132 WTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMYLSNVEKGGETV 191

Query: 185 FPNAEESQRRQASETNED-LSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPV 244
           FP      + +A++  +D  ++CAK+G AVKPRKGDALLFF+LHPNA  D+ SLHG CPV
Sbjct: 192 FP----MWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPV 251

Query: 245 IEGEKWSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYC 303
           +EGEKWSAT+WIHV SF+   +  + C+D N SCE+WA+ GEC  NP YMVGS +  GYC
Sbjct: 252 VEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKNPTYMVGSDKDHGYC 311

BLAST of Cp4.1LG08g04410 vs. Swiss-Prot
Match: P4H6_ARATH (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana GN=P4H6 PE=2 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.1e-92
Identity = 174/293 (59.39%), Postives = 212/293 (72.35%), Query Frame = 1

Query: 11  FILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAK 70
           + L+ S+SLLL     S   S S  V+P ++ Q+SW+PRAF+Y+GFL+D ECDHLI LAK
Sbjct: 5   YFLAFSLSLLL---IFSQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAK 64

Query: 71  AELKRS-AVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQ 130
            +L++S  VAD  SGES+ SEVRTSSG F+ K +D IV+ +E K+AAWTFLP+ENGE +Q
Sbjct: 65  GKLEKSMVVADVDSGESEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQ 124

Query: 131 VLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQAS 190
           +L YE GQKYD H+DYF DK  +  GGHR+ATVLMYLSNV KGGETVFPN    + +   
Sbjct: 125 ILHYENGQKYDPHFDYFYDKKALELGGHRIATVLMYLSNVTKGGETVFPN---WKGKTPQ 184

Query: 191 ETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHV 250
             ++  S CAK+G AVKPRKGDALLFF+LH N   D  SLHG CPVIEGEKWSAT+WIHV
Sbjct: 185 LKDDSWSKCAKQGYAVKPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHV 244

Query: 251 DSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
            SF         CVD++ SC+ WA+ GEC  NP YMVGS    G+CRKSCK C
Sbjct: 245 RSFG---KKKLVCVDDHESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

BLAST of Cp4.1LG08g04410 vs. Swiss-Prot
Match: P4H3_ARATH (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.3e-65
Identity = 118/209 (56.46%), Postives = 153/209 (73.21%), Query Frame = 1

Query: 44  ISWSPRAFVYEGFLTDLECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAK 103
           +SW PRAFVY  FL+  EC++LISLAK  + +S V D  +G+SK S VRTSSG F+ + +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 104 DPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVL 163
           D I+  IE +IA +TF+P ++GE +QVL YE GQKY+ HYDYF D+ N   GG RMAT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 164 MYLSNVEKGGETVFPNAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAV 223
           MYLS+VE+GGETVFP A  +    +     +LS+C KKG++VKPR GDALLF+S+ P+A 
Sbjct: 199 MYLSDVEEGGETVFPAA--NMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDAT 258

Query: 224 PDTKSLHGGCPVIEGEKWSATKWIHVDSF 253
            D  SLHGGCPVI G KWS+TKW+HV  +
Sbjct: 259 LDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

BLAST of Cp4.1LG08g04410 vs. TrEMBL
Match: A0A0A0L5Q6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G150820 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 2.9e-156
Identity = 270/302 (89.40%), Postives = 286/302 (94.70%), Query Frame = 1

Query: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+    NLLF+ ++SIS LL+RAS+SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 37  MAELLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 96

Query: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRS+VAD+LSG+SKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 97  ECDHLISLAKAELKRSSVADNLSGKSKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 156

Query: 121 PKENGEDIQVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180
           PK+NGEDIQVLRYEYGQKYD H+DYF DKVNIARGGHRMATVLMYLS+VEKGGETVFP+A
Sbjct: 157 PKDNGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 216

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNA+PDT SLHGGCPVIEGEK
Sbjct: 217 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 276

Query: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWI VDSFD +V DHT+C D N SCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 277 WSATKWIRVDSFDMVVRDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 336

Query: 301 VC 303
            C
Sbjct: 337 AC 338

BLAST of Cp4.1LG08g04410 vs. TrEMBL
Match: B9RSW4_RICCO (Prolyl 4-hydroxylase alpha subunit, putative OS=Ricinus communis GN=RCOM_0679070 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 3.4e-133
Identity = 227/292 (77.74%), Postives = 259/292 (88.70%), Query Frame = 1

Query: 11  FILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAK 70
           F+  + ISL+  + SSSY GS +SI++P+KVKQ+SW PRAFVYEGFLTDLECDHLISLAK
Sbjct: 7   FVFLLLISLIFHK-SSSYPGSPTSIIDPSKVKQVSWKPRAFVYEGFLTDLECDHLISLAK 66

Query: 71  AELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQV 130
           +ELKRSAVAD+ SG+SK+SEVRTSSG FI K KDPI++GIE+KI+ WTFLPKENGED+QV
Sbjct: 67  SELKRSAVADNESGKSKLSEVRTSSGMFIAKGKDPIIAGIEEKISTWTFLPKENGEDLQV 126

Query: 131 LRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQASE 190
           LRYE+GQKYD HYDYF DK+NIARGGHRMATVLMYLS+V KGGETVFPNAEE  RR+A+E
Sbjct: 127 LRYEHGQKYDPHYDYFADKINIARGGHRMATVLMYLSDVVKGGETVFPNAEEPPRRKATE 186

Query: 191 TNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHVD 250
           ++EDLS+CAKKGI+VKPR+GDALLFFSLHP A+PD  SLH GCPVIEGEKWSATKWIHVD
Sbjct: 187 SHEDLSECAKKGISVKPRRGDALLFFSLHPTAIPDPNSLHAGCPVIEGEKWSATKWIHVD 246

Query: 251 SFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           SFD  +    +C D N SCERWA LGECTNNPEYMVGSPELPGYCR+SCKVC
Sbjct: 247 SFDKNIEAGGNCTDKNESCERWAALGECTNNPEYMVGSPELPGYCRRSCKVC 297

BLAST of Cp4.1LG08g04410 vs. TrEMBL
Match: A0A067L7I1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02052 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 7.6e-133
Identity = 232/294 (78.91%), Postives = 260/294 (88.44%), Query Frame = 1

Query: 9   LLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISL 68
           L F+  +SISL+L + S SY G++SSI++PAKVKQ+SW PRAFVY GFLTDLECDHLISL
Sbjct: 8   LQFLFLLSISLILHK-SGSYPGTSSSIIDPAKVKQVSWKPRAFVYHGFLTDLECDHLISL 67

Query: 69  AKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDI 128
           AK+ELKRSAVAD++SG+SKV+EVRTSSG FI K KDPIV+GIEDKIA WTFLPKENGEDI
Sbjct: 68  AKSELKRSAVADNVSGKSKVAEVRTSSGMFIPKGKDPIVAGIEDKIATWTFLPKENGEDI 127

Query: 129 QVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQA 188
           QVLRYEYGQKYD HYDYF D+VNIARGGHR+ATVLMYLSNVEKGGETVFP+AE++ RR+A
Sbjct: 128 QVLRYEYGQKYDPHYDYFVDRVNIARGGHRLATVLMYLSNVEKGGETVFPSAEDAPRRKA 187

Query: 189 SETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIH 248
           +E +EDLS+CAKKGIAVKPR+GDALLFFSL PNAVPD  SLH GCPVIEGEKWSATKWIH
Sbjct: 188 NEGDEDLSECAKKGIAVKPRRGDALLFFSLLPNAVPDQSSLHAGCPVIEGEKWSATKWIH 247

Query: 249 VDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           VDSF   +    +C D N SCERWA LGECT NPEYMVGS ELPGYCR+SCKVC
Sbjct: 248 VDSFSKNLEADGNCTDLNESCERWAALGECTKNPEYMVGSAELPGYCRRSCKVC 300

BLAST of Cp4.1LG08g04410 vs. TrEMBL
Match: W9SGN4_9ROSA (Prolyl 4-hydroxylase subunit alpha-1 OS=Morus notabilis GN=L484_011286 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 9.9e-133
Identity = 231/293 (78.84%), Postives = 258/293 (88.05%), Query Frame = 1

Query: 10  LFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLA 69
           LF+  +SIS     +SSSYAGSA+SI+NP+KVKQ+SW PRAFVYEGFLTDLECDHLISLA
Sbjct: 8   LFLFLLSISSSFHESSSSYAGSAASIINPSKVKQVSWKPRAFVYEGFLTDLECDHLISLA 67

Query: 70  KAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQ 129
           K+ELKRSAVAD++SG+SK+SEVRTSSG FI KAKDPIV+GIEDKI+ WTFLPKENGED+Q
Sbjct: 68  KSELKRSAVADNVSGKSKLSEVRTSSGMFIPKAKDPIVAGIEDKISTWTFLPKENGEDMQ 127

Query: 130 VLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQAS 189
           VLRYE+GQKYD HYDYF DKVNIARGGHR+ATVLMYL++V KGGETVFP+AEES   +AS
Sbjct: 128 VLRYEHGQKYDPHYDYFADKVNIARGGHRIATVLMYLTDVVKGGETVFPSAEESHHHKAS 187

Query: 190 ETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHV 249
            T++DLS+CAKKGIAVKPR+GDALLFFSL P AVPDT SLH GCPVIEGEKWSATKWIHV
Sbjct: 188 TTDDDLSECAKKGIAVKPRRGDALLFFSLLPTAVPDTISLHAGCPVIEGEKWSATKWIHV 247

Query: 250 DSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           DSFD  +S    C D N SCERWA LGEC  N EYMVGSPELPGYCR+SCKVC
Sbjct: 248 DSFDKDLSAGGKCTDQNESCERWAALGECNKNREYMVGSPELPGYCRRSCKVC 300

BLAST of Cp4.1LG08g04410 vs. TrEMBL
Match: I1KN53_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G253200 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 1.7e-132
Identity = 231/294 (78.57%), Postives = 257/294 (87.41%), Query Frame = 1

Query: 9   LLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISL 68
           LLF+L IS         SSYAGSASS++NP+KVKQISW PRAFVYEGFLTDLECDHLISL
Sbjct: 7   LLFLLLIS---KCDHVWSSYAGSASSVINPSKVKQISWKPRAFVYEGFLTDLECDHLISL 66

Query: 69  AKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDI 128
           AK+ELKRSAVAD+LSGES++S+VRTSSG FI K KDPIV+GIEDKI++WTFLPKENGEDI
Sbjct: 67  AKSELKRSAVADNLSGESQLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDI 126

Query: 129 QVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQA 188
           QVLRYE+GQKYD HYDYFTDKVNIARGGHR+ATVLMYL++V KGGETVFP+AEE  RR+ 
Sbjct: 127 QVLRYEHGQKYDPHYDYFTDKVNIARGGHRIATVLMYLTDVAKGGETVFPSAEEPPRRRG 186

Query: 189 SETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIH 248
           +ET+ DLS+CAKKGIAVKPR+GDALLFFSLH NA PDT SLH GCPVIEGEKWSATKWIH
Sbjct: 187 AETSSDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPVIEGEKWSATKWIH 246

Query: 249 VDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           VDSFD  V     C DN+ SCERWA LGECT NPEYM+GS ++PGYCRKSCK C
Sbjct: 247 VDSFDKTVGAGGDCSDNHVSCERWASLGECTKNPEYMIGSSDIPGYCRKSCKAC 297

BLAST of Cp4.1LG08g04410 vs. TAIR10
Match: AT5G18900.1 (AT5G18900.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 462.2 bits (1188), Expect = 2.4e-130
Identity = 223/293 (76.11%), Postives = 248/293 (84.64%), Query Frame = 1

Query: 10  LFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLA 69
           L I   +I  +L ++S+S   S+S  VNP+KVKQ+S  PRAFVYEGFLT+LECDH++SLA
Sbjct: 6   LLISFFAIFSVLLQSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLA 65

Query: 70  KAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQ 129
           KA LKRSAVAD+ SGESK SEVRTSSG FI K KDPIVSGIEDKI+ WTFLPKENGEDIQ
Sbjct: 66  KASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQ 125

Query: 130 VLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQAS 189
           VLRYE+GQKYD H+DYF DKVNI RGGHRMAT+LMYLSNV KGGETVFP+AE   RR  S
Sbjct: 126 VLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLS 185

Query: 190 ETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHV 249
           E  EDLSDCAK+GIAVKPRKGDALLFF+LHP+A+PD  SLHGGCPVIEGEKWSATKWIHV
Sbjct: 186 ENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV 245

Query: 250 DSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           DSFD IV+   +C D N SCERWA LGECT NPEYMVG+ ELPGYCR+SCK C
Sbjct: 246 DSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

BLAST of Cp4.1LG08g04410 vs. TAIR10
Match: AT3G06300.1 (AT3G06300.1 P4H isoform 2)

HSP 1 Score: 449.1 bits (1154), Expect = 2.1e-126
Identity = 213/291 (73.20%), Postives = 249/291 (85.57%), Query Frame = 1

Query: 12  ILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAKA 71
           +L ++I L+L ++S+    S SSI+NP+KVKQ+S  PRAFVYEGFLTDLECDHLISLAK 
Sbjct: 9   LLFVAILLVLLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKE 68

Query: 72  ELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVL 131
            L+RSAVAD+ +GES+VS+VRTSSG FI K KDPIVSGIEDK++ WTFLPKENGED+QVL
Sbjct: 69  NLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVL 128

Query: 132 RYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQASET 191
           RYE+GQKYD H+DYF DKVNIARGGHR+ATVL+YLSNV KGGETVFP+A+E  RR  SE 
Sbjct: 129 RYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSEN 188

Query: 192 NEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHVDS 251
            +DLSDCAKKGIAVKP+KG+ALLFF+L  +A+PD  SLHGGCPVIEGEKWSATKWIHVDS
Sbjct: 189 KDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDS 248

Query: 252 FDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           FD I++   +C D N SCERWA LGEC  NPEYMVG+PE+PG CR+SCK C
Sbjct: 249 FDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

BLAST of Cp4.1LG08g04410 vs. TAIR10
Match: AT3G28490.1 (AT3G28490.1 Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 341.3 bits (874), Expect = 6.2e-94
Identity = 174/293 (59.39%), Postives = 212/293 (72.35%), Query Frame = 1

Query: 11  FILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAK 70
           + L+ S+SLLL     S   S S  V+P ++ Q+SW+PRAF+Y+GFL+D ECDHLI LAK
Sbjct: 5   YFLAFSLSLLL---IFSQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAK 64

Query: 71  AELKRS-AVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQ 130
            +L++S  VAD  SGES+ SEVRTSSG F+ K +D IV+ +E K+AAWTFLP+ENGE +Q
Sbjct: 65  GKLEKSMVVADVDSGESEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQ 124

Query: 131 VLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQAS 190
           +L YE GQKYD H+DYF DK  +  GGHR+ATVLMYLSNV KGGETVFPN    + +   
Sbjct: 125 ILHYENGQKYDPHFDYFYDKKALELGGHRIATVLMYLSNVTKGGETVFPN---WKGKTPQ 184

Query: 191 ETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHV 250
             ++  S CAK+G AVKPRKGDALLFF+LH N   D  SLHG CPVIEGEKWSAT+WIHV
Sbjct: 185 LKDDSWSKCAKQGYAVKPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHV 244

Query: 251 DSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
            SF         CVD++ SC+ WA+ GEC  NP YMVGS    G+CRKSCK C
Sbjct: 245 RSFG---KKKLVCVDDHESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

BLAST of Cp4.1LG08g04410 vs. TAIR10
Match: AT3G28480.2 (AT3G28480.2 Oxoglutarate/iron-dependent oxygenase)

HSP 1 Score: 340.1 bits (871), Expect = 1.4e-93
Identity = 175/315 (55.56%), Postives = 222/315 (70.48%), Query Frame = 1

Query: 5   CSCNLLFILSISISLLLRRASSSYAGS-------ASSI-VNPAKVKQISWSPRAFVYEGF 64
           C    L ++S + +  L R+S++  GS       ASS   +P +V Q+SW+PR F+YEGF
Sbjct: 12  CFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRVFLYEGF 71

Query: 65  LTDLECDHLISLAKAELKRSAVADHLSGES-----KVSEVRTSSGAFIHKAK---DPIVS 124
           L+D ECDH I LAK +L++S VAD+ SGES      VS VR SS    +      D IVS
Sbjct: 72  LSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSSFIANMDSLEIDDIVS 131

Query: 125 GIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSN 184
            +E K+AAWTFLP+ENGE +Q+L YE GQKY+ H+DYF D+ N+  GGHR+ATVLMYLSN
Sbjct: 132 NVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMYLSN 191

Query: 185 VEKGGETVFPNAEESQRRQASETNEDL-SDCAKKGIAVKPRKGDALLFFSLHPNAVPDTK 244
           VEKGGETVFP      + +A++  +D  ++CAK+G AVKPRKGDALLFF+LHPNA  D+ 
Sbjct: 192 VEKGGETVFP----MWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDSN 251

Query: 245 SLHGGCPVIEGEKWSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVG 303
           SLHG CPV+EGEKWSAT+WIHV SF+   +  + C+D N SCE+WA+ GEC  NP YMVG
Sbjct: 252 SLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKNPTYMVG 311

BLAST of Cp4.1LG08g04410 vs. TAIR10
Match: AT1G20270.1 (AT1G20270.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 250.0 bits (637), Expect = 1.9e-66
Identity = 118/209 (56.46%), Postives = 153/209 (73.21%), Query Frame = 1

Query: 44  ISWSPRAFVYEGFLTDLECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAK 103
           +SW PRAFVY  FL+  EC++LISLAK  + +S V D  +G+SK S VRTSSG F+ + +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 104 DPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVL 163
           D I+  IE +IA +TF+P ++GE +QVL YE GQKY+ HYDYF D+ N   GG RMAT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 164 MYLSNVEKGGETVFPNAEESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAV 223
           MYLS+VE+GGETVFP A  +    +     +LS+C KKG++VKPR GDALLF+S+ P+A 
Sbjct: 199 MYLSDVEEGGETVFPAA--NMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDAT 258

Query: 224 PDTKSLHGGCPVIEGEKWSATKWIHVDSF 253
            D  SLHGGCPVI G KWS+TKW+HV  +
Sbjct: 259 LDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

BLAST of Cp4.1LG08g04410 vs. NCBI nr
Match: gi|659076596|ref|XP_008438765.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo])

HSP 1 Score: 567.4 bits (1461), Expect = 1.5e-158
Identity = 274/302 (90.73%), Postives = 288/302 (95.36%), Query Frame = 1

Query: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+F   NLLF+ +++IS LLRRAS+SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRS+VAD+LSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180
           PKENGEDIQVLRYEYGQKYD H+DYF DKVNIARGGHRMATVLMYLS+VEKGGETVFP+A
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEK 240
           EESQRRQASETN+DLSDCAKKGIAVKPRKGDALLFFSLHPNA+PDT SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI  DHT+C D N SCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
            C
Sbjct: 301 AC 302

BLAST of Cp4.1LG08g04410 vs. NCBI nr
Match: gi|778678671|ref|XP_004134175.2| (PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis sativus])

HSP 1 Score: 559.3 bits (1440), Expect = 4.1e-156
Identity = 270/302 (89.40%), Postives = 286/302 (94.70%), Query Frame = 1

Query: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+    NLLF+ ++SIS LL+RAS+SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 37  MAELLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 96

Query: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRS+VAD+LSG+SKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 97  ECDHLISLAKAELKRSSVADNLSGKSKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 156

Query: 121 PKENGEDIQVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180
           PK+NGEDIQVLRYEYGQKYD H+DYF DKVNIARGGHRMATVLMYLS+VEKGGETVFP+A
Sbjct: 157 PKDNGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 216

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNA+PDT SLHGGCPVIEGEK
Sbjct: 217 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 276

Query: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWI VDSFD +V DHT+C D N SCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 277 WSATKWIRVDSFDMVVRDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 336

Query: 301 VC 303
            C
Sbjct: 337 AC 338

BLAST of Cp4.1LG08g04410 vs. NCBI nr
Match: gi|255551575|ref|XP_002516833.1| (PREDICTED: probable prolyl 4-hydroxylase 4 [Ricinus communis])

HSP 1 Score: 482.6 bits (1241), Expect = 4.9e-133
Identity = 227/292 (77.74%), Postives = 259/292 (88.70%), Query Frame = 1

Query: 11  FILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAK 70
           F+  + ISL+  + SSSY GS +SI++P+KVKQ+SW PRAFVYEGFLTDLECDHLISLAK
Sbjct: 7   FVFLLLISLIFHK-SSSYPGSPTSIIDPSKVKQVSWKPRAFVYEGFLTDLECDHLISLAK 66

Query: 71  AELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQV 130
           +ELKRSAVAD+ SG+SK+SEVRTSSG FI K KDPI++GIE+KI+ WTFLPKENGED+QV
Sbjct: 67  SELKRSAVADNESGKSKLSEVRTSSGMFIAKGKDPIIAGIEEKISTWTFLPKENGEDLQV 126

Query: 131 LRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQASE 190
           LRYE+GQKYD HYDYF DK+NIARGGHRMATVLMYLS+V KGGETVFPNAEE  RR+A+E
Sbjct: 127 LRYEHGQKYDPHYDYFADKINIARGGHRMATVLMYLSDVVKGGETVFPNAEEPPRRKATE 186

Query: 191 TNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIHVD 250
           ++EDLS+CAKKGI+VKPR+GDALLFFSLHP A+PD  SLH GCPVIEGEKWSATKWIHVD
Sbjct: 187 SHEDLSECAKKGISVKPRRGDALLFFSLHPTAIPDPNSLHAGCPVIEGEKWSATKWIHVD 246

Query: 251 SFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           SFD  +    +C D N SCERWA LGECTNNPEYMVGSPELPGYCR+SCKVC
Sbjct: 247 SFDKNIEAGGNCTDKNESCERWAALGECTNNPEYMVGSPELPGYCRRSCKVC 297

BLAST of Cp4.1LG08g04410 vs. NCBI nr
Match: gi|950996201|ref|XP_014505644.1| (PREDICTED: probable prolyl 4-hydroxylase 4 [Vigna radiata var. radiata])

HSP 1 Score: 482.3 bits (1240), Expect = 6.4e-133
Identity = 233/294 (79.25%), Postives = 257/294 (87.41%), Query Frame = 1

Query: 9   LLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISL 68
           LLF+L IS         SSYAGSASSI+NP+KVKQ+SW PRAFVYEGFLTDLECDHLIS+
Sbjct: 7   LLFLLLIS---KCDEVWSSYAGSASSIINPSKVKQVSWKPRAFVYEGFLTDLECDHLISI 66

Query: 69  AKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDI 128
           AK+ELKRSAVAD+LSGES +S+VRTSSG FI K KDPI+SGIEDKI++WTFLPKENGEDI
Sbjct: 67  AKSELKRSAVADNLSGESTLSDVRTSSGMFISKNKDPIISGIEDKISSWTFLPKENGEDI 126

Query: 129 QVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQA 188
           QVLRYE+GQKYD HYDYFTDKVNI RGGHR+ATVLMYL+NV KGGETVFP+AEES RR++
Sbjct: 127 QVLRYEHGQKYDPHYDYFTDKVNIVRGGHRVATVLMYLTNVTKGGETVFPSAEESPRRRS 186

Query: 189 SETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIH 248
           SET+ DLS+CAKKGIAVKPR+GDALLFFSLH NA PDT SLH GCPVIEGEKWSATKWIH
Sbjct: 187 SETSIDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPVIEGEKWSATKWIH 246

Query: 249 VDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           VDSFD  V D   C D + SCERWA LGECTNNPEYM+GS +L GYCRKSCK C
Sbjct: 247 VDSFDKTVGDGGDCSDRHVSCERWASLGECTNNPEYMIGSSDLLGYCRKSCKAC 297

BLAST of Cp4.1LG08g04410 vs. NCBI nr
Match: gi|802578382|ref|XP_012069451.1| (PREDICTED: probable prolyl 4-hydroxylase 4 [Jatropha curcas])

HSP 1 Score: 481.5 bits (1238), Expect = 1.1e-132
Identity = 232/294 (78.91%), Postives = 260/294 (88.44%), Query Frame = 1

Query: 9   LLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISL 68
           L F+  +SISL+L + S SY G++SSI++PAKVKQ+SW PRAFVY GFLTDLECDHLISL
Sbjct: 8   LQFLFLLSISLILHK-SGSYPGTSSSIIDPAKVKQVSWKPRAFVYHGFLTDLECDHLISL 67

Query: 69  AKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDI 128
           AK+ELKRSAVAD++SG+SKV+EVRTSSG FI K KDPIV+GIEDKIA WTFLPKENGEDI
Sbjct: 68  AKSELKRSAVADNVSGKSKVAEVRTSSGMFIPKGKDPIVAGIEDKIATWTFLPKENGEDI 127

Query: 129 QVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNAEESQRRQA 188
           QVLRYEYGQKYD HYDYF D+VNIARGGHR+ATVLMYLSNVEKGGETVFP+AE++ RR+A
Sbjct: 128 QVLRYEYGQKYDPHYDYFVDRVNIARGGHRLATVLMYLSNVEKGGETVFPSAEDAPRRKA 187

Query: 189 SETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEKWSATKWIH 248
           +E +EDLS+CAKKGIAVKPR+GDALLFFSL PNAVPD  SLH GCPVIEGEKWSATKWIH
Sbjct: 188 NEGDEDLSECAKKGIAVKPRRGDALLFFSLLPNAVPDQSSLHAGCPVIEGEKWSATKWIH 247

Query: 249 VDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           VDSF   +    +C D N SCERWA LGECT NPEYMVGS ELPGYCR+SCKVC
Sbjct: 248 VDSFSKNLEADGNCTDLNESCERWAALGECTKNPEYMVGSAELPGYCRRSCKVC 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P4H4_ARATH4.3e-12976.11Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana GN=P4H4 PE=2 SV=1[more]
P4H2_ARATH3.7e-12573.20Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana GN=P4H2 PE=1 SV=1[more]
P4H7_ARATH6.0e-9958.31Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana GN=P4H7 PE=2 SV=1[more]
P4H6_ARATH1.1e-9259.39Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana GN=P4H6 PE=2 SV=1[more]
P4H3_ARATH3.3e-6556.46Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L5Q6_CUCSA2.9e-15689.40Uncharacterized protein OS=Cucumis sativus GN=Csa_3G150820 PE=4 SV=1[more]
B9RSW4_RICCO3.4e-13377.74Prolyl 4-hydroxylase alpha subunit, putative OS=Ricinus communis GN=RCOM_0679070... [more]
A0A067L7I1_JATCU7.6e-13378.91Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02052 PE=4 SV=1[more]
W9SGN4_9ROSA9.9e-13378.84Prolyl 4-hydroxylase subunit alpha-1 OS=Morus notabilis GN=L484_011286 PE=4 SV=1[more]
I1KN53_SOYBN1.7e-13278.57Uncharacterized protein OS=Glycine max GN=GLYMA_07G253200 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G18900.12.4e-13076.11 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT3G06300.12.1e-12673.20 P4H isoform 2[more]
AT3G28490.16.2e-9459.39 Oxoglutarate/iron-dependent oxygenase[more]
AT3G28480.21.4e-9355.56 Oxoglutarate/iron-dependent oxygenase[more]
AT1G20270.11.9e-6656.46 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
Match NameE-valueIdentityDescription
gi|659076596|ref|XP_008438765.1|1.5e-15890.73PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo][more]
gi|778678671|ref|XP_004134175.2|4.1e-15689.40PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis sativus][more]
gi|255551575|ref|XP_002516833.1|4.9e-13377.74PREDICTED: probable prolyl 4-hydroxylase 4 [Ricinus communis][more]
gi|950996201|ref|XP_014505644.1|6.4e-13379.25PREDICTED: probable prolyl 4-hydroxylase 4 [Vigna radiata var. radiata][more]
gi|802578382|ref|XP_012069451.1|1.1e-13278.91PREDICTED: probable prolyl 4-hydroxylase 4 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0031418L-ascorbic acid binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR006620Pro_4_hyd_alph
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR003582ShKT_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006525 arginine metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline
biological_process GO:0006560 proline metabolic process
biological_process GO:0000160 phosphorelay signal transduction system
biological_process GO:0019511 peptidyl-proline hydroxylation
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0004656 procollagen-proline 4-dioxygenase activity
molecular_function GO:0004871 signal transducer activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g04410.1Cp4.1LG08g04410.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003582ShKT domainPFAMPF01549ShKcoord: 261..302
score: 1.
IPR003582ShKT domainPROFILEPS51670SHKTcoord: 262..302
score: 8
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF136402OG-FeII_Oxy_3coord: 128..248
score: 2.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 124..249
score: 12
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 48..248
score: 6.8
NoneNo IPR availablePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 25..284
score: 1.3E
NoneNo IPR availablePANTHERPTHR10869:SF64OXIDOREDUCTASE, 2OG-FE(II) OXYGENASE FAMILY PROTEIN-RELATEDcoord: 25..284
score: 1.3E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG08g04410Cucsa.253720Cucumber (Gy14) v1cgycpeB0687
Cp4.1LG08g04410CmaCh06G014510Cucurbita maxima (Rimu)cmacpeB853
Cp4.1LG08g04410CmaCh08G011910Cucurbita maxima (Rimu)cmacpeB931
Cp4.1LG08g04410CmaCh14G016540Cucurbita maxima (Rimu)cmacpeB292
Cp4.1LG08g04410CmaCh17G002910Cucurbita maxima (Rimu)cmacpeB412
Cp4.1LG08g04410CmoCh17G002790Cucurbita moschata (Rifu)cmocpeB373
Cp4.1LG08g04410CmoCh08G011630Cucurbita moschata (Rifu)cmocpeB866
Cp4.1LG08g04410CmoCh14G017000Cucurbita moschata (Rifu)cmocpeB254
Cp4.1LG08g04410CmoCh06G014520Cucurbita moschata (Rifu)cmocpeB796
Cp4.1LG08g04410Cla009353Watermelon (97103) v1cpewmB853
Cp4.1LG08g04410Cla021690Watermelon (97103) v1cpewmB845
Cp4.1LG08g04410Csa3G150820Cucumber (Chinese Long) v2cpecuB859
Cp4.1LG08g04410MELO3C021380Melon (DHL92) v3.5.1cpemeB793
Cp4.1LG08g04410MELO3C006592Melon (DHL92) v3.5.1cpemeB813
Cp4.1LG08g04410ClCG05G005650Watermelon (Charleston Gray)cpewcgB787
Cp4.1LG08g04410ClCG06G005580Watermelon (Charleston Gray)cpewcgB788
Cp4.1LG08g04410CSPI03G13780Wild cucumber (PI 183967)cpecpiB861
Cp4.1LG08g04410Lsi09G013810Bottle gourd (USVL1VR-Ls)cpelsiB697
Cp4.1LG08g04410Lsi05G014850Bottle gourd (USVL1VR-Ls)cpelsiB718
Cp4.1LG08g04410MELO3C006592.2Melon (DHL92) v3.6.1cpemedB951
Cp4.1LG08g04410MELO3C021380.2Melon (DHL92) v3.6.1cpemedB933
Cp4.1LG08g04410CsaV3_3G014000Cucumber (Chinese Long) v3cpecucB1056
Cp4.1LG08g04410CsaV3_6G005580Cucumber (Chinese Long) v3cpecucB1081
Cp4.1LG08g04410Bhi01G000661Wax gourdcpewgoB1109
Cp4.1LG08g04410Bhi12G001572Wax gourdcpewgoB1096
Cp4.1LG08g04410CsGy6G005200Cucumber (Gy14) v2cgybcpeB894
Cp4.1LG08g04410CsGy3G013910Cucumber (Gy14) v2cgybcpeB454
Cp4.1LG08g04410Carg17382Silver-seed gourdcarcpeB0861
Cp4.1LG08g04410Carg05473Silver-seed gourdcarcpeB0109
Cp4.1LG08g04410Carg15947Silver-seed gourdcarcpeB0456
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG08g04410Cp4.1LG12g02390Cucurbita pepo (Zucchini)cpecpeB194
Cp4.1LG08g04410Cp4.1LG17g01080Cucurbita pepo (Zucchini)cpecpeB342
Cp4.1LG08g04410Cp4.1LG03g11130Cucurbita pepo (Zucchini)cpecpeB482
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG08g04410Cucumber (Gy14) v2cgybcpeB885
Cp4.1LG08g04410Cucumber (Gy14) v2cgybcpeB989
Cp4.1LG08g04410Silver-seed gourdcarcpeB1165
Cp4.1LG08g04410Cucumber (Chinese Long) v3cpecucB1073
Cp4.1LG08g04410Wax gourdcpewgoB1110