Cp4.1LG07g03330 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG07g03330
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionGDSL esterase/lipase
LocationCp4.1LG07 : 2236455 .. 2239882 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAACTACACAATAGCAATGCCTTTACTCCACTTCACAATAGCACTCAAGCTCTTCCTTTGTCTTCAACTAGCTAGTGCTACTATAAACATTCCTCCCGGTTACTCGGTCCCCGCGGTTTTCGTGTTCGGGGATTCGATCGTCGATACTGGCAACAATAACAATCTCATCACTCAAGCTAAGTGCAACTATCCGCCGTACGGTAGGAATTTTCTCGATCAACAGGCAACTGGGCGATTTTCCAATGGCAAAGTTCCCTCCGACCTTCTAGGTCTAAACTAACCTCGTTCGATTAAATTGTAGAGGCTCAAATTTTTTTCAGTTACGTGGCTTGGACCAATGTTATCGGTTTCTTTTTGTTTGTTTTTTGTTAGTTGATGCGTTGGGTATCAAAGAGTTTTTACCACCGTATGCTAACCCGAGTCTACGGCCCGAAGACCTAATTACGGGTGTCAATTTTGCTTCTGGTGGTGCTGGATTTGATCCTCTGACCTCTCAATTAGCGGTAATGAATAAACTTTGAACACGGGACGATATTTTGAATGATACGCTAAATGGGTTCTTGAATTTTCAGCCAGCAATATCACTTGAAGATCAATTAGGCATGTTTAGGGAATACTCAAAGAAGCTAGAAGAACTAGTGGGAGTAGCAAGAGCAAAGTTCATAATAGCCAATGGCTTGTTCATGGTGGTGGCGGGAAGCAACGACATTGCCAACACATTTTATCTTGCAAGAATAAGGCAAATTCATTTCAATATCGACTCGTACACTGATTTCATGGCTCGGTATGCTTCCGCTTATGCTAAGGTACAAAGTTTAGTCTCTCGACTTTCATATTTATATTCAATTTCTATTTTTTAATTTAAAAAATATTTAGTTTCTGTGCAATGGAGTGGAACGAAACATTCCTTACAAGTAGATGCGTTTTAAAACCTTGAGTGAAAGCCGGGAGGGAAAGTTCAAAGAAGACAATATCTTCTAGTGATGGGCTTGGGCTGTTACAAATGGTATTAGAGCTAGACATCGGGTGGCGTGCCAGCGAGGATGCTGGGCCTCCAAGGGAAGTGGATTGTGAGATCCTACATCGGTTGGAGAGGGGAACGTTCCTTATAAGGGTGTGGAAACCTCTTCCTAGTAGACGTGTTTTAAAACCTTGAGTGAAAGCCCGAAAGGGAAAGCCCAAAGAAGACAATATCTAGGGCGATTTTCCAATGGCAAAGTTCCCTCCGACCTTCTAGGTCTAAACTAACCTCGTTCGATTAAATTGTAGAGGCTCAAATTTTTTTCAGTTACGTGGCTTGGACCAATGTTATCGGTTTCTTTTTGTTTGTTTTTTGTTAGTTGATGCGTTGGGTATCAAAGAGTTTTTACCACCGTATGCTAACCCGAGTCTACGGCCCGAAGACCTAATTACGGGTGTCAATTTTGCTTCTGGTGGTGCTGGATTTGATCCTCTGACCTCTCAATTAGCGGTAATGAATAAACTTTGAACACGGGACGATATTTTGAATGATACGCTAAATGGGTTCTTGAATTTTCAGCCAGCAATATCACTTGAAGATCAATTAGGCATGTTTAGGGAATACTCAAAGAAGCTAGAAGAACTAGTGGGAGTAGCAAGAGCAAAGTTCATAATAGCCAATGGCTTGTTCATGGTGGTGGCGGGAAGCAACGACATAGCCAACACATTTTATCTTGCAAGAATAAGGCAAATTCATTTCAATATCGACTCGTACACTGATTTCATGGCTCGGTATGCTTCCGCTTTTGCTAAGGTACAAAGTTTAGTCTCTCGACTTTCATATTTATATTCAATTTCTATTTTTTAATTTAAAAAATATTTAGTTTCTGTGCAATGGAGTGGAACGAAACATTCCTTACAAGTAGATGCGTTTTAAAACCTTGAGTGAAAGCCGGGAGGGAAAGTTCAAAGAAGACAATATCTTCTAGTGATGGGCTTGGGCTGTTACAAATGGTATTAGAGCTAGACATCGGGTGGCGTGCCAGCGAGGATGCTGGGCCTCCAAGAGAAGTGGATTGTGAGATCCTACATCGGTTGGAGAGGGGAACGTTCCTTATAAGGGTGTGGAAACCTCTTCCTAGTAGACATGTTTTAAAACCTTGAGTGAAAGCCCGAAAGGGAAAGCCCAAAGAAGACAATATCTACTAGCGATGGGCTTGGGCTGTTACAAATGGTATTAGAGCTCGACACCGGGTGGCGTGTCAGCGAGGATGCTGGGCCTCCAAGAGAAGTGGATTGTGAGATCCTACATCGGTTGGAGAGGGGAACGTTCCTTATAAGGGTGTGGAAACCTCTTCCTAGTAGACATGTTTTAAAACCTTGAGTGAAAGCCCGAAAGGGAAAGCCCAAAGAAGACAATATCTGTTAGCAATGGGCTTGGACTGTTACAAATGGTATTAGAGCTCGACACCGGGCGATGTGCCAACGAGGATGCTGGGCCCCCAAGGGAAGTGGATTGTGAGATCCTACACTGGTTGGAGAGGGGAACGTTTCTTATAAGGGTATGGAAACCTCTCCCGAGTAGACGCGAGGAAAGTCCAAAGAGCACCACATCTATCGGTGGGAGCTTGATTTAATATTAATTAATTAAATGCATTTTGTTTCATTCATCACATGCAAGTACCTTATCTTTTTCATCTAAATTTTAACATTTTATTTTCTTTAAAGGAATTGTACGCAGCCGGTGGGCGGAGAATCGGATTCTTCGGGGCACCGCCGTTAGGGTGTGTGCCGTCGCAAAGAACCTTGGCTGGCGGGATTGAAAGGGCGTGCGTGAATGAGTACAACGCTGCTGCAGAGCTCTTCAATGGAAAATTGCACATTGCTTTGAATCAACTTCAAACGTCACTGCCTAACAGCAAACTAGTTTACGTGGATATTTACAATCCTCTTCTTGACGTCATCCAAAACTATCGTAAATATGGTATTCAATCATCTCTCCCTCTCCATCTACTGAAAATTAGTGTAGACGCTCGTATGACTATTCTACTGAAAATAATAGCAAATCGATCACAAGCTTAACCGAACGATTTGAAAAGCTGAAAACGTTCGTAATTGTTATTATTTTAGGGTTTGAAGTTGCGGATAGAGGGTGTTGTGGAACTGGGAAGATCGAGGTGACGTTTCTTTGTAACAAATTCGTGAAGACCTGCAACGATCCGAACAAGTTCGTGTTTTGGGACAGTTTTCATCCAACCGAAGCTACATACAGCTACCTCGTCGCTCCGATCATTCAAAAATATATTTCTAATTTCCTATAGTTCAATTAAAGTTTAATTTTCATAAAAATAATATTACAAAATTGAAGGCAAAAAAAGGATGTGTAACTTTAATTCTCTCAATTATATATGTATTATCATTTTGTTTACATTAATGATGAGCTAATAATTAATGATTTTTGAATTAA

mRNA sequence

TGAACTACACAATAGCAATGCCTTTACTCCACTTCACAATAGCACTCAAGCTCTTCCTTTGTCTTCAACTAGCTAGTGCTACTATAAACATTCCTCCCGGTTACTCGGTCCCCGCGGTTTTCGTGTTCGGGGATTCGATCGTCGATACTGGCAACAATAACAATCTCATCACTCAAGCTAAGTGCAACTATCCGCCGTACGGTAGGAATTTTCTCGATCAACAGGCAACTGGGCGATTTTCCAATGGCAAAGTTCCCTCCGACCTTCTAGTTGATGCGTTGGGTATCAAAGAGTTTTTACCACCGTATGCTAACCCGAGTCTACGGCCCGAAGACCTAATTACGGGTGTCAATTTTGCTTCTGGTGGTGCTGGATTTGATCCTCTGACCTCTCAATTAGCGCCAGCAATATCACTTGAAGATCAATTAGGCATGTTTAGGGAATACTCAAAGAAGCTAGAAGAACTAGTGGGAGTAGCAAGAGCAAAGTTCATAATAGCCAATGGCTTGTTCATGGTGGTGGCGGGAAGCAACGACATTGCCAACACATTTTATCTTGCAAGAATAAGGCAAATTCATTTCAATATCGACTCGTACACTGATTTCATGGCTCGGTATGCTTCCGCTTATGCTAAGTTACGTGGCTTGGACCAATGTTATCGGTTTCTTTTTGTTTGTTTTTTGTTAGTTGATGCGTTGGGTATCAAAGAGTTTTTACCACCGTATGCTAACCCGAGTCTACGGCCCGAAGACCTAATTACGGGTGTCAATTTTGCTTCTGGTGGTGCTGGATTTGATCCTCTGACCTCTCAATTAGCGCCAGCAATATCACTTGAAGATCAATTAGGCATGTTTAGGGAATACTCAAAGAAGCTAGAAGAACTAGTGGGAGTAGCAAGAGCAAAGTTCATAATAGCCAATGGCTTGTTCATGGTGGTGGCGGGAAGCAACGACATAGCCAACACATTTTATCTTGCAAGAATAAGGCAAATTCATTTCAATATCGACTCGTACACTGATTTCATGGCTCGGTATGCTTCCGCTTTTGCTAAGGAATTGTACGCAGCCGGTGGGCGGAGAATCGGATTCTTCGGGGCACCGCCGTTAGGGTGTGTGCCGTCGCAAAGAACCTTGGCTGGCGGGATTGAAAGGGCGTGCGTGAATGAGTACAACGCTGCTGCAGAGCTCTTCAATGGAAAATTGCACATTGCTTTGAATCAACTTCAAACGTCACTGCCTAACAGCAAACTAGTTTACGTGGATATTTACAATCCTCTTCTTGACGTCATCCAAAACTATCGTAAATATGGGTTTGAAGTTGCGGATAGAGGGTGTTGTGGAACTGGGAAGATCGAGGTGACGTTTCTTTGTAACAAATTCGTGAAGACCTGCAACGATCCGAACAAGTTCGTGTTTTGGGACAGTTTTCATCCAACCGAAGCTACATACAGCTACCTCGTCGCTCCGATCATTCAAAAATATATTTCTAATTTCCTATAGTTCAATTAAAGTTTAATTTTCATAAAAATAATATTACAAAATTGAAGGCAAAAAAAGGATGTGTAACTTTAATTCTCTCAATTATATATGTATTATCATTTTGTTTACATTAATGATGAGCTAATAATTAATGATTTTTGAATTAA

Coding sequence (CDS)

ATGCCTTTACTCCACTTCACAATAGCACTCAAGCTCTTCCTTTGTCTTCAACTAGCTAGTGCTACTATAAACATTCCTCCCGGTTACTCGGTCCCCGCGGTTTTCGTGTTCGGGGATTCGATCGTCGATACTGGCAACAATAACAATCTCATCACTCAAGCTAAGTGCAACTATCCGCCGTACGGTAGGAATTTTCTCGATCAACAGGCAACTGGGCGATTTTCCAATGGCAAAGTTCCCTCCGACCTTCTAGTTGATGCGTTGGGTATCAAAGAGTTTTTACCACCGTATGCTAACCCGAGTCTACGGCCCGAAGACCTAATTACGGGTGTCAATTTTGCTTCTGGTGGTGCTGGATTTGATCCTCTGACCTCTCAATTAGCGCCAGCAATATCACTTGAAGATCAATTAGGCATGTTTAGGGAATACTCAAAGAAGCTAGAAGAACTAGTGGGAGTAGCAAGAGCAAAGTTCATAATAGCCAATGGCTTGTTCATGGTGGTGGCGGGAAGCAACGACATTGCCAACACATTTTATCTTGCAAGAATAAGGCAAATTCATTTCAATATCGACTCGTACACTGATTTCATGGCTCGGTATGCTTCCGCTTATGCTAAGTTACGTGGCTTGGACCAATGTTATCGGTTTCTTTTTGTTTGTTTTTTGTTAGTTGATGCGTTGGGTATCAAAGAGTTTTTACCACCGTATGCTAACCCGAGTCTACGGCCCGAAGACCTAATTACGGGTGTCAATTTTGCTTCTGGTGGTGCTGGATTTGATCCTCTGACCTCTCAATTAGCGCCAGCAATATCACTTGAAGATCAATTAGGCATGTTTAGGGAATACTCAAAGAAGCTAGAAGAACTAGTGGGAGTAGCAAGAGCAAAGTTCATAATAGCCAATGGCTTGTTCATGGTGGTGGCGGGAAGCAACGACATAGCCAACACATTTTATCTTGCAAGAATAAGGCAAATTCATTTCAATATCGACTCGTACACTGATTTCATGGCTCGGTATGCTTCCGCTTTTGCTAAGGAATTGTACGCAGCCGGTGGGCGGAGAATCGGATTCTTCGGGGCACCGCCGTTAGGGTGTGTGCCGTCGCAAAGAACCTTGGCTGGCGGGATTGAAAGGGCGTGCGTGAATGAGTACAACGCTGCTGCAGAGCTCTTCAATGGAAAATTGCACATTGCTTTGAATCAACTTCAAACGTCACTGCCTAACAGCAAACTAGTTTACGTGGATATTTACAATCCTCTTCTTGACGTCATCCAAAACTATCGTAAATATGGGTTTGAAGTTGCGGATAGAGGGTGTTGTGGAACTGGGAAGATCGAGGTGACGTTTCTTTGTAACAAATTCGTGAAGACCTGCAACGATCCGAACAAGTTCGTGTTTTGGGACAGTTTTCATCCAACCGAAGCTACATACAGCTACCTCGTCGCTCCGATCATTCAAAAATATATTTCTAATTTCCTATAG

Protein sequence

MPLLHFTIALKLFLCLQLASATINIPPGYSVPAVFVFGDSIVDTGNNNNLITQAKCNYPPYGRNFLDQQATGRFSNGKVPSDLLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFREYSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYASAYAKLRGLDQCYRFLFVCFLLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFREYSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYASAFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQLQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKTCNDPNKFVFWDSFHPTEATYSYLVAPIIQKYISNFL
BLAST of Cp4.1LG07g03330 vs. Swiss-Prot
Match: EXL3_ARATH (GDSL esterase/lipase EXL3 OS=Arabidopsis thaliana GN=EXL3 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 8.1e-85
Identity = 149/273 (54.58%), Postives = 199/273 (72.89%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           LL + LGIK  +P Y +P+L+ +DL+TGV+FASGG+G+DP+T +L   ISLEDQL  F E
Sbjct: 93  LLAEELGIKSIVPAYLDPNLKSKDLLTGVSFASGGSGYDPITPKLVAVISLEDQLSYFEE 152

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y +K++ +VG AR  FI+AN LF++VAGS+DIANT+Y  R R   +++DSYT  M+  AS
Sbjct: 153 YIEKVKNIVGEARKDFIVANSLFLLVAGSDDIANTYYTLRARP-EYDVDSYTTLMSDSAS 212

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
            F  +LY  G RR+  FGAPP+GCVPSQRTL GGI R C + YN AA+LFN KL   L+ 
Sbjct: 213 EFVTKLYGYGVRRVAVFGAPPIGCVPSQRTLGGGILRDCADNYNEAAKLFNSKLSPKLDS 272

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKT-CND 461
           L+ +LP  K +Y++IY+PL D+IQN   YGFEV+++GCCGTG IEV  LCNK   + C D
Sbjct: 273 LRKTLPGIKPIYINIYDPLFDIIQNPANYGFEVSNKGCCGTGAIEVAVLCNKITSSVCPD 332

Query: 462 PNKFVFWDSFHPTEATYSYLVAPIIQKYISNFL 494
            +  VFWDS+HPTE TY  LV+ +I K+++ F+
Sbjct: 333 VSTHVFWDSYHPTEKTYKVLVSLLINKFVNQFV 364

BLAST of Cp4.1LG07g03330 vs. Swiss-Prot
Match: EXL1_ARATH (GDSL esterase/lipase EXL1 OS=Arabidopsis thaliana GN=EXL1 PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.6e-77
Identity = 141/273 (51.65%), Postives = 193/273 (70.70%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPA-ISLEDQLGMFR 281
           ++ + LGIK  +P Y NP+L+PE+L+TGV FASGGAG+ PLT+++A   I L  QL  F 
Sbjct: 102 IVAEELGIKPNIPAYRNPNLKPEELLTGVTFASGGAGYVPLTTKIAVGGIPLPQQLIYFE 161

Query: 282 EYSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYA 341
           EY +KL+++VG  R KFII N LF+V+ GSNDIAN F+     ++H+ + S+T  MA  A
Sbjct: 162 EYIEKLKQMVGEKRTKFIIKNSLFVVICGSNDIANDFFTLPPVRLHYTVASFTALMADNA 221

Query: 342 SAFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALN 401
            +FA+ LY  G RRI  FGAPP+GCVPSQRT+AGG  R CV  +N AA+LFN KL   ++
Sbjct: 222 RSFAQTLYGYGARRILVFGAPPIGCVPSQRTVAGGPTRDCVARFNDAAKLFNTKLSANID 281

Query: 402 QLQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKF-VKTCN 461
            L  +L +  ++Y+DIY+PLLD+I N  +YGF+VA++GCCGTG IEVT LCN +    C 
Sbjct: 282 VLSRTLQDPTIIYIDIYSPLLDLILNPHQYGFKVANKGCCGTGLIEVTALCNNYTASVCP 341

Query: 462 DPNKFVFWDSFHPTEATYSYLVAPIIQKYISNF 493
             + +VFWDSFHPTE  Y  +VA ++ +Y++ F
Sbjct: 342 IRSDYVFWDSFHPTEKAYRIIVAKLLDRYLNRF 374

BLAST of Cp4.1LG07g03330 vs. Swiss-Prot
Match: GDL4_ARATH (GDSL esterase/lipase At1g20120 OS=Arabidopsis thaliana GN=At1g20120 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 9.9e-75
Identity = 131/270 (48.52%), Postives = 188/270 (69.63%), Query Frame = 1

Query: 223 LVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFREY 282
           + D +G+K  +P Y  P L  EDL+TGV+FASGG+G+DPLT  +  AI +  QL  F+EY
Sbjct: 129 IADYIGVKPVVPAYLRPGLTQEDLLTGVSFASGGSGYDPLTPIVVSAIPMSKQLTYFQEY 188

Query: 283 SKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYASA 342
            +K++  VG  +A+ II+ GL +VVAGS+D+ANT+Y   + +  ++ID+YT FMA  A++
Sbjct: 189 IEKVKGFVGKEKAEHIISKGLAIVVAGSDDLANTYYGEHLEEFLYDIDTYTSFMASSAAS 248

Query: 343 FAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQL 402
           FA +LY +G ++IGF G  P+GC+P QRT  GG++R C +E N AA+LFN KL  +LN+L
Sbjct: 249 FAMQLYESGAKKIGFIGVSPIGCIPIQRTTRGGLKRKCADELNFAAQLFNSKLSTSLNEL 308

Query: 403 QTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVK-TCNDP 462
             ++ N+ LVY+DIY+   D+IQN +KYGF+  DRGCCGTG +E+  LCNK+    C + 
Sbjct: 309 AKTMKNTTLVYIDIYSSFNDMIQNPKKYGFDEIDRGCCGTGLLELGPLCNKYTSLLCKNV 368

Query: 463 NKFVFWDSFHPTEATYSYLVAPIIQKYISN 492
           + F+FWDS+HPTE  Y  L     QK++ N
Sbjct: 369 SSFMFWDSYHPTERAYKIL----SQKFVEN 394

BLAST of Cp4.1LG07g03330 vs. Swiss-Prot
Match: EXL2_ARATH (GDSL esterase/lipase EXL2 OS=Arabidopsis thaliana GN=EXL2 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 7.1e-73
Identity = 136/276 (49.28%), Postives = 183/276 (66.30%), Query Frame = 1

Query: 228 GIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAP-------------AISLED 287
           GIK  +P Y NP+L+PEDL+TGV FASGGAG+ P T+QL+               I+L  
Sbjct: 104 GIKPSIPAYRNPNLKPEDLLTGVTFASGGAGYVPFTTQLSTYLFIYKPLLFLKGGIALSQ 163

Query: 288 QLGMFREYSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTD 347
           QL +F EY +K++++VG  R K II N LFMV+ GSNDI NT++     Q  +++ S+T 
Sbjct: 164 QLKLFEEYVEKMKKMVGEERTKLIIKNSLFMVICGSNDITNTYFGLPSVQQQYDVASFTT 223

Query: 348 FMARYASAFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGK 407
            MA  A +FA++L+  G RRI  FGAPP+GCVPSQRTLAGG  R CV  +N A +L+N K
Sbjct: 224 LMADNARSFAQKLHEYGARRIQVFGAPPVGCVPSQRTLAGGPTRNCVVRFNDATKLYNVK 283

Query: 408 LHIALNQLQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKF 467
           L   L  L  +L +  ++YVDIY+ LLD+I + R+YGF+V D+GCCGTG IEV  LCN F
Sbjct: 284 LAANLGSLSRTLGDKTIIYVDIYDSLLDIILDPRQYGFKVVDKGCCGTGLIEVALLCNNF 343

Query: 468 -VKTCNDPNKFVFWDSFHPTEATYSYLVAPIIQKYI 490
               C + +++VFWDSFHPTE TY  +     ++Y+
Sbjct: 344 AADVCPNRDEYVFWDSFHPTEKTYRIMATKYFERYV 379

BLAST of Cp4.1LG07g03330 vs. Swiss-Prot
Match: GDL90_ARATH (GDSL esterase/lipase At5g42170 OS=Arabidopsis thaliana GN=At5g42170/At5g42160 PE=3 SV=2)

HSP 1 Score: 275.0 bits (702), Expect = 1.6e-72
Identity = 130/273 (47.62%), Postives = 182/273 (66.67%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           ++ + LGI E +P Y NP L+ EDL+ GVNFASGG+G+DPLT++L   +SL DQL  F+E
Sbjct: 100 IVAERLGIAETIPAYLNPKLKNEDLLKGVNFASGGSGYDPLTAKLVKVVSLSDQLKNFQE 159

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y  KL+ +VG  +A F++ N L++VVA SNDIA+T+     R I +N  SY D++A  AS
Sbjct: 160 YKNKLKVIVGEEKANFLVKNSLYLVVASSNDIAHTY---TARSIKYNKTSYADYLADSAS 219

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
            F   LY  G RRIG F A P+GCVP+ RTL G ++R C  + N  A  FN K+   L  
Sbjct: 220 KFVSALYGLGARRIGVFSAVPVGCVPAARTLRGKLKRRCSEKLNEVARNFNAKISPTLEA 279

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVK-TCND 461
           L   LP+S++V +D+ + L D+I+N + YGFEV++RGCCGTG +EV FLCNK    TC +
Sbjct: 280 LGKELPDSRVVLIDVCDTLNDMIENPKNYGFEVSNRGCCGTGLVEVLFLCNKINPFTCKN 339

Query: 462 PNKFVFWDSFHPTEATYSYLVAPIIQKYISNFL 494
            + ++FWDS+HPTE  Y  +V  ++  YI+  +
Sbjct: 340 SSSYIFWDSYHPTEKAYQIIVDKLLGNYITKLV 369

BLAST of Cp4.1LG07g03330 vs. TrEMBL
Match: A0A0A0LN84_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G346080 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 6.6e-118
Identity = 206/272 (75.74%), Postives = 235/272 (86.40%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           L+VD LGIK  LPPYA+P+L+ EDL+TGVNFASGGAGFDPLTS+ APAISL+ QL MFRE
Sbjct: 101 LVVDVLGIKPLLPPYADPNLQLEDLLTGVNFASGGAGFDPLTSKTAPAISLDAQLAMFRE 160

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y KK+E LVG  +AKFII N LF+VVAGSNDI NTFYLAR RQ  +NID+YTDFM ++AS
Sbjct: 161 YRKKIEGLVGEEKAKFIIDNSLFLVVAGSNDIGNTFYLARFRQGQYNIDTYTDFMIQHAS 220

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
           A+ K+LYAAG RRIGFF  PPLGC+PSQRTLAGGIER CVNEYN AA+LFNGKL   L  
Sbjct: 221 AYVKDLYAAGARRIGFFATPPLGCLPSQRTLAGGIERGCVNEYNNAAKLFNGKLQTTLGY 280

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKTCNDP 461
           LQT LP+S++VYVDIYNPLLDVIQNY KYGFEV D+GCCGTG IEVTFLCNKFVKTC D 
Sbjct: 281 LQTILPDSRVVYVDIYNPLLDVIQNYAKYGFEVVDKGCCGTGTIEVTFLCNKFVKTCPDT 340

Query: 462 NKFVFWDSFHPTEATYSYLVAPIIQKYISNFL 494
            K+VFWDSFHP+EATY+ LV+PII++YIS+FL
Sbjct: 341 TKYVFWDSFHPSEATYNLLVSPIIKRYISSFL 372

BLAST of Cp4.1LG07g03330 vs. TrEMBL
Match: A0A078FJ25_BRANA (BnaC02g29810D protein OS=Brassica napus GN=BnaC02g29810D PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.4e-112
Identity = 218/490 (44.49%), Postives = 314/490 (64.08%), Query Frame = 1

Query: 22  TINIPPGYSVPAVFVFGDSIVDTGNNNNLITQAKCNYPPYGRNFLDQQATGRFSNGKVPS 81
           TI +P   ++P V  FGDSIVD+GNNNNL T  KCN+PPYG++F  + ATGRF++G+VPS
Sbjct: 63  TIKLPRNVTIPGVIAFGDSIVDSGNNNNLRTILKCNFPPYGQDFQGKFATGRFTDGRVPS 122

Query: 82  DLLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFR 141
           D + + LGI + +P Y +P+L+ +DL+ G+NFASGG+G+DPLT+++   +SLEDQL  F+
Sbjct: 123 DFVAERLGIAKTIPAYLDPALKGKDLLKGINFASGGSGYDPLTAKIVRVVSLEDQLKYFQ 182

Query: 142 EYSKKLEELVGVARAKFIIANGLFMVVAGSN--------------------DIANTFYLA 201
           EY +K++ +VG  +AKF++ N L++VVA                       D  N   L 
Sbjct: 183 EYKEKIKAIVGEEKAKFMVENSLYLVVASITIKLPRNVTIPGVIAFGDSIVDSGNNNNLR 242

Query: 202 RIRQIHFNIDSY-TDFMARYASAYAKLRGLDQCYRFLFVCFLLVDALGIKEFLPPYANPS 261
            I  +  N   Y  DF  ++A+     R  D      FV     + LGI + +P Y +P+
Sbjct: 243 TI--LKSNFPPYGQDFRGKFATG----RFTDGRVPSDFV----AERLGIAKTIPAYLDPA 302

Query: 262 LRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFREYSKKLEELVGVARAKFIIA 321
           L+ +DL+ G+NFASGG+G+DPLT+++   +SLEDQL  F+EY +K++ +VG  +AKF++ 
Sbjct: 303 LKGKDLLKGINFASGGSGYDPLTAKIVRVVSLEDQLKYFQEYKEKIKAIVGEEKAKFMVE 362

Query: 322 NGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYASAFAKELYAAGGRRIGFFGA 381
           N L++VVA SND+A+T Y+A  R + +N   Y D++A  +S F K+LY  G RRI  F A
Sbjct: 363 NSLYLVVASSNDLAHT-YIA--RSLRYNSTEYPDYLADMSSKFVKKLYGLGARRIAIFSA 422

Query: 382 PPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQLQTSLPNSKLVYVDIYNPL 441
            P+GC+PS+RTL  G++R C    N  A  FN KL   L  L+   P SK+V+VD+Y+ L
Sbjct: 423 VPVGCLPSRRTL-NGVKRKCSENLNKMALQFNAKLSPGLVALRKKSPGSKIVFVDVYDIL 482

Query: 442 LDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVK-TCNDPNKFVFWDSFHPTEATYSY 490
            ++I+N +KYGFEVA+RG CGTG  EV  LCNK    TC + +  VFWDS+HPTE  Y  
Sbjct: 483 HNMIENPKKYGFEVANRG-CGTGLFEVLILCNKINPFTCKNASSHVFWDSYHPTEKAYQV 537

BLAST of Cp4.1LG07g03330 vs. TrEMBL
Match: V7AR92_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G018200g PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.0e-94
Identity = 164/271 (60.52%), Postives = 209/271 (77.12%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           LLV+ LGIK  LP Y +P+L+P+DL+TGV FASG +G+DPLT ++A  IS+ +Q+ MF+E
Sbjct: 92  LLVEELGIKNLLPAYLDPNLKPDDLVTGVCFASGASGYDPLTPKIASVISMSEQIEMFKE 151

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y  KL+++VGV R  FI+AN  F+VVAGS+DIANT+++AR RQ+ ++I +YTD M   AS
Sbjct: 152 YIGKLKQIVGVDRTNFILANSFFLVVAGSDDIANTYFVARARQLQYDIPAYTDLMCNSAS 211

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
            F KELY  G RRIG  GAPP+GCVPSQRTLAGG+ER C  EYN AA+LFN KL   L+ 
Sbjct: 212 DFVKELYGLGARRIGVLGAPPIGCVPSQRTLAGGLERECAEEYNYAAKLFNSKLSRKLDS 271

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKTCNDP 461
           L + LPNS++VY+DIYNPL+D+I NY +YG+EV D GCCGTGK+EV  LCN    TC+D 
Sbjct: 272 L-SHLPNSRIVYIDIYNPLMDIIVNYHRYGYEVMDSGCCGTGKLEVAVLCNHLDSTCSDA 331

Query: 462 NKFVFWDSFHPTEATYSYLVAPIIQKYISNF 493
           +K+VFWDS+HPTE  Y  LVA I+Q YIS F
Sbjct: 332 SKYVFWDSYHPTEKVYRQLVAGILQNYISRF 361

BLAST of Cp4.1LG07g03330 vs. TrEMBL
Match: A0A151RUV0_CAJCA (GDSL esterase/lipase EXL3 OS=Cajanus cajan GN=KK1_032116 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.7e-94
Identity = 161/271 (59.41%), Postives = 207/271 (76.38%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           LLV+ LGIKE LP Y +P+L+P DL+TGV FASG  G+DPLT ++   IS+ DQL MF+E
Sbjct: 74  LLVEELGIKELLPAYLDPNLKPSDLVTGVCFASGATGYDPLTPKITSVISMSDQLEMFKE 133

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y  KL+ +VG  RA FI+AN  ++VVAGS+DIANT+++AR+RQ+ ++I SYTD M   AS
Sbjct: 134 YIGKLKNIVGEERANFILANSFYLVVAGSDDIANTYFIARVRQLQYDIPSYTDLMLNSAS 193

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
            F KELY  G RRIG   APP+GCVPSQRTLAGGI+R C   YN AA+LFN KL   LN 
Sbjct: 194 DFVKELYKLGARRIGVLSAPPIGCVPSQRTLAGGIQRDCAENYNYAAKLFNSKLSRELNS 253

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKTCNDP 461
           L  +LP+S++VY+D+YNPLLD+I NY++YG++VADRGCCGTGK+EV+ LCN    TC D 
Sbjct: 254 LNYNLPDSRIVYIDVYNPLLDIIVNYQRYGYKVADRGCCGTGKLEVSVLCNHLDGTCPDD 313

Query: 462 NKFVFWDSFHPTEATYSYLVAPIIQKYISNF 493
            ++VFWDS+HPTE  Y  L+  I+QKY++ F
Sbjct: 314 TEYVFWDSYHPTERVYRQLIVQILQKYLTRF 344

BLAST of Cp4.1LG07g03330 vs. TrEMBL
Match: A0A0B2SM62_GLYSO (GDSL esterase/lipase EXL3 OS=Glycine soja GN=glysoja_023161 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 6.6e-94
Identity = 157/271 (57.93%), Postives = 209/271 (77.12%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           LLV+ LGIKE LP Y +P+L+P DL+TGV FASG +G+DPLT ++A  IS+ +QL MF+E
Sbjct: 93  LLVEELGIKELLPAYLDPNLKPSDLVTGVCFASGASGYDPLTPKIASVISMSEQLDMFKE 152

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y  KL+ +VG  R KFI+AN  F+VVAGS+DIANT+++AR+RQ+ ++I +YTD M   AS
Sbjct: 153 YIGKLKHIVGEDRTKFILANSFFLVVAGSDDIANTYFIARVRQLQYDIPAYTDLMLHSAS 212

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
            F KELY  G RRIG   APP+GCVPSQRTLAGG +R C  EYN AA+LFN KL   L+ 
Sbjct: 213 NFVKELYGLGARRIGVLSAPPIGCVPSQRTLAGGFQRECAEEYNYAAKLFNSKLSRELDA 272

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKTCNDP 461
           L+ +LPNS++VY+D+YNPL+D+I NY+++G++V DRGCCGTGK+EV  LCN    TC D 
Sbjct: 273 LKHNLPNSRIVYIDVYNPLMDIIVNYQRHGYKVVDRGCCGTGKLEVAVLCNPLGATCPDA 332

Query: 462 NKFVFWDSFHPTEATYSYLVAPIIQKYISNF 493
           +++VFWDS+HPTE  Y  L+  ++QKY++ F
Sbjct: 333 SQYVFWDSYHPTEGVYRQLIVQVLQKYLTRF 363

BLAST of Cp4.1LG07g03330 vs. TAIR10
Match: AT1G75900.1 (AT1G75900.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 315.8 bits (808), Expect = 4.6e-86
Identity = 149/273 (54.58%), Postives = 199/273 (72.89%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           LL + LGIK  +P Y +P+L+ +DL+TGV+FASGG+G+DP+T +L   ISLEDQL  F E
Sbjct: 93  LLAEELGIKSIVPAYLDPNLKSKDLLTGVSFASGGSGYDPITPKLVAVISLEDQLSYFEE 152

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y +K++ +VG AR  FI+AN LF++VAGS+DIANT+Y  R R   +++DSYT  M+  AS
Sbjct: 153 YIEKVKNIVGEARKDFIVANSLFLLVAGSDDIANTYYTLRARP-EYDVDSYTTLMSDSAS 212

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
            F  +LY  G RR+  FGAPP+GCVPSQRTL GGI R C + YN AA+LFN KL   L+ 
Sbjct: 213 EFVTKLYGYGVRRVAVFGAPPIGCVPSQRTLGGGILRDCADNYNEAAKLFNSKLSPKLDS 272

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKT-CND 461
           L+ +LP  K +Y++IY+PL D+IQN   YGFEV+++GCCGTG IEV  LCNK   + C D
Sbjct: 273 LRKTLPGIKPIYINIYDPLFDIIQNPANYGFEVSNKGCCGTGAIEVAVLCNKITSSVCPD 332

Query: 462 PNKFVFWDSFHPTEATYSYLVAPIIQKYISNFL 494
            +  VFWDS+HPTE TY  LV+ +I K+++ F+
Sbjct: 333 VSTHVFWDSYHPTEKTYKVLVSLLINKFVNQFV 364

BLAST of Cp4.1LG07g03330 vs. TAIR10
Match: AT1G75880.1 (AT1G75880.1 SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 291.6 bits (745), Expect = 9.2e-79
Identity = 141/273 (51.65%), Postives = 193/273 (70.70%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPA-ISLEDQLGMFR 281
           ++ + LGIK  +P Y NP+L+PE+L+TGV FASGGAG+ PLT+++A   I L  QL  F 
Sbjct: 102 IVAEELGIKPNIPAYRNPNLKPEELLTGVTFASGGAGYVPLTTKIAVGGIPLPQQLIYFE 161

Query: 282 EYSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYA 341
           EY +KL+++VG  R KFII N LF+V+ GSNDIAN F+     ++H+ + S+T  MA  A
Sbjct: 162 EYIEKLKQMVGEKRTKFIIKNSLFVVICGSNDIANDFFTLPPVRLHYTVASFTALMADNA 221

Query: 342 SAFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALN 401
            +FA+ LY  G RRI  FGAPP+GCVPSQRT+AGG  R CV  +N AA+LFN KL   ++
Sbjct: 222 RSFAQTLYGYGARRILVFGAPPIGCVPSQRTVAGGPTRDCVARFNDAAKLFNTKLSANID 281

Query: 402 QLQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKF-VKTCN 461
            L  +L +  ++Y+DIY+PLLD+I N  +YGF+VA++GCCGTG IEVT LCN +    C 
Sbjct: 282 VLSRTLQDPTIIYIDIYSPLLDLILNPHQYGFKVANKGCCGTGLIEVTALCNNYTASVCP 341

Query: 462 DPNKFVFWDSFHPTEATYSYLVAPIIQKYISNF 493
             + +VFWDSFHPTE  Y  +VA ++ +Y++ F
Sbjct: 342 IRSDYVFWDSFHPTEKAYRIIVAKLLDRYLNRF 374

BLAST of Cp4.1LG07g03330 vs. TAIR10
Match: AT1G20120.1 (AT1G20120.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 282.3 bits (721), Expect = 5.6e-76
Identity = 131/270 (48.52%), Postives = 188/270 (69.63%), Query Frame = 1

Query: 223 LVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFREY 282
           + D +G+K  +P Y  P L  EDL+TGV+FASGG+G+DPLT  +  AI +  QL  F+EY
Sbjct: 129 IADYIGVKPVVPAYLRPGLTQEDLLTGVSFASGGSGYDPLTPIVVSAIPMSKQLTYFQEY 188

Query: 283 SKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYASA 342
            +K++  VG  +A+ II+ GL +VVAGS+D+ANT+Y   + +  ++ID+YT FMA  A++
Sbjct: 189 IEKVKGFVGKEKAEHIISKGLAIVVAGSDDLANTYYGEHLEEFLYDIDTYTSFMASSAAS 248

Query: 343 FAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQL 402
           FA +LY +G ++IGF G  P+GC+P QRT  GG++R C +E N AA+LFN KL  +LN+L
Sbjct: 249 FAMQLYESGAKKIGFIGVSPIGCIPIQRTTRGGLKRKCADELNFAAQLFNSKLSTSLNEL 308

Query: 403 QTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVK-TCNDP 462
             ++ N+ LVY+DIY+   D+IQN +KYGF+  DRGCCGTG +E+  LCNK+    C + 
Sbjct: 309 AKTMKNTTLVYIDIYSSFNDMIQNPKKYGFDEIDRGCCGTGLLELGPLCNKYTSLLCKNV 368

Query: 463 NKFVFWDSFHPTEATYSYLVAPIIQKYISN 492
           + F+FWDS+HPTE  Y  L     QK++ N
Sbjct: 369 SSFMFWDSYHPTERAYKIL----SQKFVEN 394

BLAST of Cp4.1LG07g03330 vs. TAIR10
Match: AT1G75890.1 (AT1G75890.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 276.2 bits (705), Expect = 4.0e-74
Identity = 136/276 (49.28%), Postives = 183/276 (66.30%), Query Frame = 1

Query: 228 GIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAP-------------AISLED 287
           GIK  +P Y NP+L+PEDL+TGV FASGGAG+ P T+QL+               I+L  
Sbjct: 104 GIKPSIPAYRNPNLKPEDLLTGVTFASGGAGYVPFTTQLSTYLFIYKPLLFLKGGIALSQ 163

Query: 288 QLGMFREYSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTD 347
           QL +F EY +K++++VG  R K II N LFMV+ GSNDI NT++     Q  +++ S+T 
Sbjct: 164 QLKLFEEYVEKMKKMVGEERTKLIIKNSLFMVICGSNDITNTYFGLPSVQQQYDVASFTT 223

Query: 348 FMARYASAFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGK 407
            MA  A +FA++L+  G RRI  FGAPP+GCVPSQRTLAGG  R CV  +N A +L+N K
Sbjct: 224 LMADNARSFAQKLHEYGARRIQVFGAPPVGCVPSQRTLAGGPTRNCVVRFNDATKLYNVK 283

Query: 408 LHIALNQLQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKF 467
           L   L  L  +L +  ++YVDIY+ LLD+I + R+YGF+V D+GCCGTG IEV  LCN F
Sbjct: 284 LAANLGSLSRTLGDKTIIYVDIYDSLLDIILDPRQYGFKVVDKGCCGTGLIEVALLCNNF 343

Query: 468 -VKTCNDPNKFVFWDSFHPTEATYSYLVAPIIQKYI 490
               C + +++VFWDSFHPTE TY  +     ++Y+
Sbjct: 344 AADVCPNRDEYVFWDSFHPTEKTYRIMATKYFERYV 379

BLAST of Cp4.1LG07g03330 vs. TAIR10
Match: AT5G42170.1 (AT5G42170.1 SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 275.0 bits (702), Expect = 8.9e-74
Identity = 130/273 (47.62%), Postives = 182/273 (66.67%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           ++ + LGI E +P Y NP L+ EDL+ GVNFASGG+G+DPLT++L   +SL DQL  F+E
Sbjct: 100 IVAERLGIAETIPAYLNPKLKNEDLLKGVNFASGGSGYDPLTAKLVKVVSLSDQLKNFQE 159

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y  KL+ +VG  +A F++ N L++VVA SNDIA+T+     R I +N  SY D++A  AS
Sbjct: 160 YKNKLKVIVGEEKANFLVKNSLYLVVASSNDIAHTY---TARSIKYNKTSYADYLADSAS 219

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
            F   LY  G RRIG F A P+GCVP+ RTL G ++R C  + N  A  FN K+   L  
Sbjct: 220 KFVSALYGLGARRIGVFSAVPVGCVPAARTLRGKLKRRCSEKLNEVARNFNAKISPTLEA 279

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVK-TCND 461
           L   LP+S++V +D+ + L D+I+N + YGFEV++RGCCGTG +EV FLCNK    TC +
Sbjct: 280 LGKELPDSRVVLIDVCDTLNDMIENPKNYGFEVSNRGCCGTGLVEVLFLCNKINPFTCKN 339

Query: 462 PNKFVFWDSFHPTEATYSYLVAPIIQKYISNFL 494
            + ++FWDS+HPTE  Y  +V  ++  YI+  +
Sbjct: 340 SSSYIFWDSYHPTEKAYQIIVDKLLGNYITKLV 369

BLAST of Cp4.1LG07g03330 vs. NCBI nr
Match: gi|449450488|ref|XP_004142994.1| (PREDICTED: GDSL esterase/lipase EXL3 [Cucumis sativus])

HSP 1 Score: 432.6 bits (1111), Expect = 9.5e-118
Identity = 206/272 (75.74%), Postives = 235/272 (86.40%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           L+VD LGIK  LPPYA+P+L+ EDL+TGVNFASGGAGFDPLTS+ APAISL+ QL MFRE
Sbjct: 101 LVVDVLGIKPLLPPYADPNLQLEDLLTGVNFASGGAGFDPLTSKTAPAISLDAQLAMFRE 160

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y KK+E LVG  +AKFII N LF+VVAGSNDI NTFYLAR RQ  +NID+YTDFM ++AS
Sbjct: 161 YRKKIEGLVGEEKAKFIIDNSLFLVVAGSNDIGNTFYLARFRQGQYNIDTYTDFMIQHAS 220

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
           A+ K+LYAAG RRIGFF  PPLGC+PSQRTLAGGIER CVNEYN AA+LFNGKL   L  
Sbjct: 221 AYVKDLYAAGARRIGFFATPPLGCLPSQRTLAGGIERGCVNEYNNAAKLFNGKLQTTLGY 280

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKTCNDP 461
           LQT LP+S++VYVDIYNPLLDVIQNY KYGFEV D+GCCGTG IEVTFLCNKFVKTC D 
Sbjct: 281 LQTILPDSRVVYVDIYNPLLDVIQNYAKYGFEVVDKGCCGTGTIEVTFLCNKFVKTCPDT 340

Query: 462 NKFVFWDSFHPTEATYSYLVAPIIQKYISNFL 494
            K+VFWDSFHP+EATY+ LV+PII++YIS+FL
Sbjct: 341 TKYVFWDSFHPSEATYNLLVSPIIKRYISSFL 372

BLAST of Cp4.1LG07g03330 vs. NCBI nr
Match: gi|659089087|ref|XP_008445321.1| (PREDICTED: GDSL esterase/lipase EXL3-like [Cucumis melo])

HSP 1 Score: 432.6 bits (1111), Expect = 9.5e-118
Identity = 206/272 (75.74%), Postives = 237/272 (87.13%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           L+VD LGIK  LPPYA+P+L+ EDL+TGVNFASGGAGFDPLTS+LAPAISL+ QL MFRE
Sbjct: 103 LVVDVLGIKPLLPPYADPNLQLEDLLTGVNFASGGAGFDPLTSKLAPAISLDAQLAMFRE 162

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y KK+E LVG  +AKFII N LF+VVAGSNDI NTFYLAR RQ  +NID+YTDFM ++AS
Sbjct: 163 YRKKIEGLVGEEKAKFIIDNSLFLVVAGSNDIGNTFYLARFRQGQYNIDTYTDFMNQHAS 222

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
           A+ K+LYAAG RRIGFF  PPLGC+PSQRTLAGGI+RACVNEYN AA+LFNGKL   L+ 
Sbjct: 223 AYVKDLYAAGARRIGFFATPPLGCLPSQRTLAGGIQRACVNEYNDAAKLFNGKLQTTLSY 282

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKTCNDP 461
           LQ++LP+S++VYVDIYNPLLDVIQNY KYGFEVADRGCCGTG IEVT LCNK VKTC D 
Sbjct: 283 LQSTLPDSRVVYVDIYNPLLDVIQNYAKYGFEVADRGCCGTGTIEVTLLCNKLVKTCPDT 342

Query: 462 NKFVFWDSFHPTEATYSYLVAPIIQKYISNFL 494
            K+VFWDSFHP+E TY+ LV+PIIQ+YIS+FL
Sbjct: 343 TKYVFWDSFHPSETTYNLLVSPIIQRYISSFL 374

BLAST of Cp4.1LG07g03330 vs. NCBI nr
Match: gi|674920249|emb|CDY12974.1| (BnaC02g29810D [Brassica napus])

HSP 1 Score: 414.1 bits (1063), Expect = 3.5e-112
Identity = 218/490 (44.49%), Postives = 314/490 (64.08%), Query Frame = 1

Query: 22  TINIPPGYSVPAVFVFGDSIVDTGNNNNLITQAKCNYPPYGRNFLDQQATGRFSNGKVPS 81
           TI +P   ++P V  FGDSIVD+GNNNNL T  KCN+PPYG++F  + ATGRF++G+VPS
Sbjct: 63  TIKLPRNVTIPGVIAFGDSIVDSGNNNNLRTILKCNFPPYGQDFQGKFATGRFTDGRVPS 122

Query: 82  DLLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFR 141
           D + + LGI + +P Y +P+L+ +DL+ G+NFASGG+G+DPLT+++   +SLEDQL  F+
Sbjct: 123 DFVAERLGIAKTIPAYLDPALKGKDLLKGINFASGGSGYDPLTAKIVRVVSLEDQLKYFQ 182

Query: 142 EYSKKLEELVGVARAKFIIANGLFMVVAGSN--------------------DIANTFYLA 201
           EY +K++ +VG  +AKF++ N L++VVA                       D  N   L 
Sbjct: 183 EYKEKIKAIVGEEKAKFMVENSLYLVVASITIKLPRNVTIPGVIAFGDSIVDSGNNNNLR 242

Query: 202 RIRQIHFNIDSY-TDFMARYASAYAKLRGLDQCYRFLFVCFLLVDALGIKEFLPPYANPS 261
            I  +  N   Y  DF  ++A+     R  D      FV     + LGI + +P Y +P+
Sbjct: 243 TI--LKSNFPPYGQDFRGKFATG----RFTDGRVPSDFV----AERLGIAKTIPAYLDPA 302

Query: 262 LRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFREYSKKLEELVGVARAKFIIA 321
           L+ +DL+ G+NFASGG+G+DPLT+++   +SLEDQL  F+EY +K++ +VG  +AKF++ 
Sbjct: 303 LKGKDLLKGINFASGGSGYDPLTAKIVRVVSLEDQLKYFQEYKEKIKAIVGEEKAKFMVE 362

Query: 322 NGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYASAFAKELYAAGGRRIGFFGA 381
           N L++VVA SND+A+T Y+A  R + +N   Y D++A  +S F K+LY  G RRI  F A
Sbjct: 363 NSLYLVVASSNDLAHT-YIA--RSLRYNSTEYPDYLADMSSKFVKKLYGLGARRIAIFSA 422

Query: 382 PPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQLQTSLPNSKLVYVDIYNPL 441
            P+GC+PS+RTL  G++R C    N  A  FN KL   L  L+   P SK+V+VD+Y+ L
Sbjct: 423 VPVGCLPSRRTL-NGVKRKCSENLNKMALQFNAKLSPGLVALRKKSPGSKIVFVDVYDIL 482

Query: 442 LDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVK-TCNDPNKFVFWDSFHPTEATYSY 490
            ++I+N +KYGFEVA+RG CGTG  EV  LCNK    TC + +  VFWDS+HPTE  Y  
Sbjct: 483 HNMIENPKKYGFEVANRG-CGTGLFEVLILCNKINPFTCKNASSHVFWDSYHPTEKAYQV 537

BLAST of Cp4.1LG07g03330 vs. NCBI nr
Match: gi|593267855|ref|XP_007136105.1| (hypothetical protein PHAVU_009G018200g [Phaseolus vulgaris])

HSP 1 Score: 355.5 bits (911), Expect = 1.5e-94
Identity = 164/271 (60.52%), Postives = 209/271 (77.12%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           LLV+ LGIK  LP Y +P+L+P+DL+TGV FASG +G+DPLT ++A  IS+ +Q+ MF+E
Sbjct: 92  LLVEELGIKNLLPAYLDPNLKPDDLVTGVCFASGASGYDPLTPKIASVISMSEQIEMFKE 151

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y  KL+++VGV R  FI+AN  F+VVAGS+DIANT+++AR RQ+ ++I +YTD M   AS
Sbjct: 152 YIGKLKQIVGVDRTNFILANSFFLVVAGSDDIANTYFVARARQLQYDIPAYTDLMCNSAS 211

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
            F KELY  G RRIG  GAPP+GCVPSQRTLAGG+ER C  EYN AA+LFN KL   L+ 
Sbjct: 212 DFVKELYGLGARRIGVLGAPPIGCVPSQRTLAGGLERECAEEYNYAAKLFNSKLSRKLDS 271

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKTCNDP 461
           L + LPNS++VY+DIYNPL+D+I NY +YG+EV D GCCGTGK+EV  LCN    TC+D 
Sbjct: 272 L-SHLPNSRIVYIDIYNPLMDIIVNYHRYGYEVMDSGCCGTGKLEVAVLCNHLDSTCSDA 331

Query: 462 NKFVFWDSFHPTEATYSYLVAPIIQKYISNF 493
           +K+VFWDS+HPTE  Y  LVA I+Q YIS F
Sbjct: 332 SKYVFWDSYHPTEKVYRQLVAGILQNYISRF 361

BLAST of Cp4.1LG07g03330 vs. NCBI nr
Match: gi|1012334969|gb|KYP46313.1| (GDSL esterase/lipase EXL3 [Cajanus cajan])

HSP 1 Score: 354.8 bits (909), Expect = 2.5e-94
Identity = 161/271 (59.41%), Postives = 207/271 (76.38%), Query Frame = 1

Query: 222 LLVDALGIKEFLPPYANPSLRPEDLITGVNFASGGAGFDPLTSQLAPAISLEDQLGMFRE 281
           LLV+ LGIKE LP Y +P+L+P DL+TGV FASG  G+DPLT ++   IS+ DQL MF+E
Sbjct: 74  LLVEELGIKELLPAYLDPNLKPSDLVTGVCFASGATGYDPLTPKITSVISMSDQLEMFKE 133

Query: 282 YSKKLEELVGVARAKFIIANGLFMVVAGSNDIANTFYLARIRQIHFNIDSYTDFMARYAS 341
           Y  KL+ +VG  RA FI+AN  ++VVAGS+DIANT+++AR+RQ+ ++I SYTD M   AS
Sbjct: 134 YIGKLKNIVGEERANFILANSFYLVVAGSDDIANTYFIARVRQLQYDIPSYTDLMLNSAS 193

Query: 342 AFAKELYAAGGRRIGFFGAPPLGCVPSQRTLAGGIERACVNEYNAAAELFNGKLHIALNQ 401
            F KELY  G RRIG   APP+GCVPSQRTLAGGI+R C   YN AA+LFN KL   LN 
Sbjct: 194 DFVKELYKLGARRIGVLSAPPIGCVPSQRTLAGGIQRDCAENYNYAAKLFNSKLSRELNS 253

Query: 402 LQTSLPNSKLVYVDIYNPLLDVIQNYRKYGFEVADRGCCGTGKIEVTFLCNKFVKTCNDP 461
           L  +LP+S++VY+D+YNPLLD+I NY++YG++VADRGCCGTGK+EV+ LCN    TC D 
Sbjct: 254 LNYNLPDSRIVYIDVYNPLLDIIVNYQRYGYKVADRGCCGTGKLEVSVLCNHLDGTCPDD 313

Query: 462 NKFVFWDSFHPTEATYSYLVAPIIQKYISNF 493
            ++VFWDS+HPTE  Y  L+  I+QKY++ F
Sbjct: 314 TEYVFWDSYHPTERVYRQLIVQILQKYLTRF 344

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
EXL3_ARATH8.1e-8554.58GDSL esterase/lipase EXL3 OS=Arabidopsis thaliana GN=EXL3 PE=2 SV=1[more]
EXL1_ARATH1.6e-7751.65GDSL esterase/lipase EXL1 OS=Arabidopsis thaliana GN=EXL1 PE=2 SV=1[more]
GDL4_ARATH9.9e-7548.52GDSL esterase/lipase At1g20120 OS=Arabidopsis thaliana GN=At1g20120 PE=2 SV=1[more]
EXL2_ARATH7.1e-7349.28GDSL esterase/lipase EXL2 OS=Arabidopsis thaliana GN=EXL2 PE=2 SV=1[more]
GDL90_ARATH1.6e-7247.62GDSL esterase/lipase At5g42170 OS=Arabidopsis thaliana GN=At5g42170/At5g42160 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0LN84_CUCSA6.6e-11875.74Uncharacterized protein OS=Cucumis sativus GN=Csa_2G346080 PE=4 SV=1[more]
A0A078FJ25_BRANA2.4e-11244.49BnaC02g29810D protein OS=Brassica napus GN=BnaC02g29810D PE=4 SV=1[more]
V7AR92_PHAVU1.0e-9460.52Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G018200g PE=4 SV=1[more]
A0A151RUV0_CAJCA1.7e-9459.41GDSL esterase/lipase EXL3 OS=Cajanus cajan GN=KK1_032116 PE=4 SV=1[more]
A0A0B2SM62_GLYSO6.6e-9457.93GDSL esterase/lipase EXL3 OS=Glycine soja GN=glysoja_023161 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G75900.14.6e-8654.58 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT1G75880.19.2e-7951.65 SGNH hydrolase-type esterase superfamily protein[more]
AT1G20120.15.6e-7648.52 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT1G75890.14.0e-7449.28 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT5G42170.18.9e-7447.62 SGNH hydrolase-type esterase superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449450488|ref|XP_004142994.1|9.5e-11875.74PREDICTED: GDSL esterase/lipase EXL3 [Cucumis sativus][more]
gi|659089087|ref|XP_008445321.1|9.5e-11875.74PREDICTED: GDSL esterase/lipase EXL3-like [Cucumis melo][more]
gi|674920249|emb|CDY12974.1|3.5e-11244.49BnaC02g29810D [Brassica napus][more]
gi|593267855|ref|XP_007136105.1|1.5e-9460.52hypothetical protein PHAVU_009G018200g [Phaseolus vulgaris][more]
gi|1012334969|gb|KYP46313.1|2.5e-9459.41GDSL esterase/lipase EXL3 [Cajanus cajan][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016298lipase activity
GO:0016788hydrolase activity, acting on ester bonds
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR008265Lipase_GDSL_AS
IPR001087GDSL
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016298 lipase activity
molecular_function GO:0016635 oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor
molecular_function GO:0016788 hydrolase activity, acting on ester bonds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g03330.1Cp4.1LG07g03330.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 34..480
score: 6.5
IPR008265Lipase, GDSL, active sitePROSITEPS01098LIPASE_GDSL_SERcoord: 34..45
scor
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 224..491
score: 8.8E-189coord: 6..84
score: 8.8E
NoneNo IPR availablePANTHERPTHR22835:SF184GDSL ESTERASE/LIPASE EXL1-RELATEDcoord: 6..84
score: 8.8E-189coord: 224..491
score: 8.8E