Cp4.1LG06g08860 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG06g08860
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCytochrome P450
LocationCp4.1LG06 : 4810835 .. 4814309 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGATGACCCTTTTCGGGAAACCGACGGTGTTTGTACATGGGGTGGCGGCGAACAAGGCGGTGGTGTTTTTGGGCGAGGAGGAGGCGGTGTCGAACCGGCAGACGGAGTCGTTGAGGAGGATCTTGGGAGAGAGGAATTTGACGGAGTTGAGTGGTGAGGATCATAAGAGGGTTAGAGGGGCATTGGTTTGGTTCTTGAGGCCTCAGAGCTTGAAAGGGTTTGTGGGGAAGATGGATGGAGAGGTTAGGAAGCATTTGAACTTGCATTGGCATGGCAACAAGGAAGTCATTGTGAGTTCTCTAAATATTAAGTTTTTTTTTAAAAAAAAAAATTATATAAATTGTTCGGGTATGACTTAGGCTGTGTTTGAGTCTGAATTTTCAAACCCGACATTGTGGCAATTTCGTGGACCAAAAATGAGATTATTTCACACTTTAGGGGCTAAAATGTCGGGGCCCAGCATCCTCGCTGACACTCATTTCCTTCTCCCATTGATGTGAGATCTCACAATCCACCCCCCTTCGGGGCAAGCATACTCGCTAACACTCGTTTCATTCTCCAATTGATGTGGGACCCCTCAATCCACCCTTCGGGGTCAGCGTCCTTGCTGGCACATCACCCAGTGTCTGGCTCTAATACCATTTCTAAAAATCCAAGCTCACCCCTAGTAGATATTGTCCTCTTGGGTTTTTCTGTTTGGGCTTTTCCTCCGGGTTTTTAAAACAGTCTACCAGGGAGAGTTTCCACACCCTTATAAAGAATGCTTCATTCTCTTCCCTAACCACGGTCAGATCTCACAATAAATAAGTTTATGAAAAACAAGTTACTAAAACTTCTTTTTTAGTTTTAAAAACCCGTAAAAAAGTAGATAACAAACGAAAAATTCAGAGGTGGAAATAGTGTCTATATCCTTAAAACTGAAAAAAAACGAAGAACAAAGGACAAAATGGTTATCACACCCTTATAAACAATACTTCATTCTCCTCCCCAATCAACGTGAGATCTCACAATAAACAAGTTTATGAAAAACAAGTTACTAAAACTTCCTTTTTAGTTTTAAAAACCCGTAAAAAGTAGATAACAAGCGAAAAATTCAGATGTGGAAATAGTGTTTATAACCTTAAAACTTCAAAAAACAAAGAACAAAGAACAAAATGGTTATCAAACGAGACCTAAATCATCGTTTTAACGTCTTAACAATGTTACAAAAACGCAGGTAGCGCCATTGATGAAAACACTAACCTTTGACATCATATGCTCTCTCCTGTTTGGGATTGAAGAAGGGCCAACAAGAAAGAGCATAATAGAATGCTTCAAAACAATGGTGGATGGGATTTGGTCTGTTCCAATCAACCTGCCATTCACAAGGTACAGCCATAGCCTCAAAGCAAGTGCAAAAGTTCAACAGATATTGAAGCAGCTTTTGAAGGACAAGGCACAAAAAATGGAAGAAGAAGAAGAAAAGGAAGAGGATAAGAACCTGATTACTTGTTTGCTTAGCATCAAGAACAAAGACAAAGAACAAGCTTTGAGTGAGGAGGAAATTGTGCACAATATCATCCTTCTATTGATTGCTGGACATGATACAACATCCATTTTGATTACTCTTATGCTTAGGGTTATGGCTACAAACCCAACTGTTTATGCAGCTGTTCTTCAAGGTATGTATGTAAATTTTTAGTTCTTTCTTATTTCTTATATATGGGGTGTCGTTTAGTTGAGATCATTGACGATTTTGAAAAAAACGAGATTCGATCTTAGTAGAATTGGGGTTTGGGGTTTGTGTTACAAGATTGGTTGAGTTATTAGGGTTTTAAGTAAGTGGAAGGGGTGAGCCGGGTGCTTGTGGGCCCCCTCTGCTAGTCCACCGCTAGCAAATATTGTCATCTTTGGGTTTTCTCTTCTGGGCTTACCCTTAAGGTTTTAAAACCAGTGAGAGGTTTCCACACCTTTATAAAGAATGTTCCGTTTTCTCCTCCAACCAATGTGGGATCTCACAATCGACCCCCTTTAGGGGCCCAGCGTCCTCACTGGCACACTGCTCGATGTCGAGCTCTGATACCATTGTAACAGCCATAGCCCACCATTAACAGATATTGCCCTCTTTGGGCTTTTCCTTTTAGGCTTCCCCTCGAGGTTTTAAAACACGTCTAATAGGGAAAGATTTTCACATTCTTATAAGGAATACTTCGCTCCCCTCTCCAACTAATGTGCGATCTCATAATCCACCTCTATTGGGGGCCAACGTCCTTGTTGGCACACCGCCTAGTGTCTGGCTCTAATCCCATTTGTAACAGCCCTAGCCTCACTGCTAGCAGATATTGTCTTATTTGGACTTTCTCTTTGGACTTCAGACGTTTTAATATCAATTTTAAACACCTTAATAGTTTTAGAATGTTGACTTTCAAAAAAGGTTTTTCAATGATTAGATAACTTCAATGGATCATTCTAATCCTAGTTCGATAAGTTATTCAGTCATTATGATGAGATTTCAAGCCATATTCGAAACTACAACTATAAGTATACTCTTCTTTTGATTGTTGTTCATTATCAGTTAGTCAAATTCATCGACCCGAGTCGAATAATAACCAAATCTACACTATTTATGGTTTTAGAACATGAAGACATACTTAGAAGCAAAGAATGTGGGGAGGCCCTCACTTGGGAAGATGTTTCCAAGATGAAGTACACATGGAGAGTAGCATTGGAGACTTTGAGACTTTTCCCACCTGTCTTTGGAGGGTTTAGAATGGCTCTCAAAGACATTCAATTAGGTGGATACACTATTCCAAAAGGATGGCAAGTAAGCAAACTTCACCTTCTTTACGGTTTCTACAATATACTTTTAAAGTTCAGGTCCTAAACTCACAAGTCTTTTACGGTTTTTACAATATTCTTTTAAAGTTCAGGTACTAAACTCATAAGTCGACGAGTATTAACTCAAACCTATTTCCCTTTTCTTTTCATTTGTCCTTCTAAACTCGACATTGATGAACTACCATTCACTAAATTCAGATATTTTGGGCGGCATCAATGACCCATTTGGACGAGAAAATATTCAGAGACCCACAAAAGTTTGAACCAAGTAGATTTGATCAGAACCAAACGTCCATTCCACCACCCTTATCTTTCATCGCCTTTGGTGGAGGACCCAGAATTTGCCCTGGCTATGAGTTTGCAAAGCTTGAAACTCTTGTCACAATTCATTACATAATCACTCAGTTCACTTGGAAGCTCTCTTCACAAGACTTCTTGACCAGAGATCCAAGCTTGATGCCCAACAAAGGGTTACCCATTCAACTTTACCCCAAATTATGAACAAACTTAGCACGAATCGACTAGTCGTGGGTGACTTGTAATGTAATATTCTATACTTTCTTTCTATGTTTAATCTTGTCTTTTATGTAGATCGTTATGTTTTCAGTTGTCTGTAATGTTGTATAAGTTTTGAGCTCATTTTTATGTGTTCTTTGTT

mRNA sequence

AGATGACCCTTTTCGGGAAACCGACGGTGTTTGTACATGGGGTGGCGGCGAACAAGGCGGTGGTGTTTTTGGGCGAGGAGGAGGCGGTGTCGAACCGGCAGACGGAGTCGTTGAGGAGGATCTTGGGAGAGAGGAATTTGACGGAGTTGAGTGGTGAGGATCATAAGAGGGTTAGAGGGGCATTGGTTTGGTTCTTGAGGCCTCAGAGCTTGAAAGGGTTTGTGGGGAAGATGGATGGAGAGGTTAGGAAGCATTTGAACTTGCATTGGCATGGCAACAAGGAAGTCATTGTAGCGCCATTGATGAAAACACTAACCTTTGACATCATATGCTCTCTCCTGTTTGGGATTGAAGAAGGGCCAACAAGAAAGAGCATAATAGAATGCTTCAAAACAATGGTGGATGGGATTTGGTCTGTTCCAATCAACCTGCCATTCACAAGGTACAGCCATAGCCTCAAAGCAAGTGCAAAAGTTCAACAGATATTGAAGCAGCTTTTGAAGGACAAGGCACAAAAAATGGAAGAAGAAGAAGAAAAGGAAGAGGATAAGAACCTGATTACTTGTTTGCTTAGCATCAAGAACAAAGACAAAGAACAAGCTTTGAGTGAGGAGGAAATTGTGCACAATATCATCCTTCTATTGATTGCTGGACATGATACAACATCCATTTTGATTACTCTTATGCTTAGGGTTATGGCTACAAACCCAACTGTTTATGCAGCTGTTCTTCAAGAACATGAAGACATACTTAGAAGCAAAGAATGTGGGGAGGCCCTCACTTGGGAAGATGTTTCCAAGATGAAGTACACATGGAGAGTAGCATTGGAGACTTTGAGACTTTTCCCACCTGTCTTTGGAGGGTTTAGAATGGCTCTCAAAGACATTCAATTAGGTGGATACACTATTCCAAAAGGATGGCAAATATTTTGGGCGGCATCAATGACCCATTTGGACGAGAAAATATTCAGAGACCCACAAAAGTTTGAACCAAGTAGATTTGATCAGAACCAAACGTCCATTCCACCACCCTTATCTTTCATCGCCTTTGGTGGAGGACCCAGAATTTGCCCTGGCTATGAGTTTGCAAAGCTTGAAACTCTTGTCACAATTCATTACATAATCACTCAGTTCACTTGGAAGCTCTCTTCACAAGACTTCTTGACCAGAGATCCAAGCTTGATGCCCAACAAAGGGTTACCCATTCAACTTTACCCCAAATTATGAACAAACTTAGCACGAATCGACTAGTCGTGGGTGACTTGTAATGTAATATTCTATACTTTCTTTCTATGTTTAATCTTGTCTTTTATGTAGATCGTTATGTTTTCAGTTGTCTGTAATGTTGTATAAGTTTTGAGCTCATTTTTATGTGTTCTTTGTT

Coding sequence (CDS)

ATGACCCTTTTCGGGAAACCGACGGTGTTTGTACATGGGGTGGCGGCGAACAAGGCGGTGGTGTTTTTGGGCGAGGAGGAGGCGGTGTCGAACCGGCAGACGGAGTCGTTGAGGAGGATCTTGGGAGAGAGGAATTTGACGGAGTTGAGTGGTGAGGATCATAAGAGGGTTAGAGGGGCATTGGTTTGGTTCTTGAGGCCTCAGAGCTTGAAAGGGTTTGTGGGGAAGATGGATGGAGAGGTTAGGAAGCATTTGAACTTGCATTGGCATGGCAACAAGGAAGTCATTGTAGCGCCATTGATGAAAACACTAACCTTTGACATCATATGCTCTCTCCTGTTTGGGATTGAAGAAGGGCCAACAAGAAAGAGCATAATAGAATGCTTCAAAACAATGGTGGATGGGATTTGGTCTGTTCCAATCAACCTGCCATTCACAAGGTACAGCCATAGCCTCAAAGCAAGTGCAAAAGTTCAACAGATATTGAAGCAGCTTTTGAAGGACAAGGCACAAAAAATGGAAGAAGAAGAAGAAAAGGAAGAGGATAAGAACCTGATTACTTGTTTGCTTAGCATCAAGAACAAAGACAAAGAACAAGCTTTGAGTGAGGAGGAAATTGTGCACAATATCATCCTTCTATTGATTGCTGGACATGATACAACATCCATTTTGATTACTCTTATGCTTAGGGTTATGGCTACAAACCCAACTGTTTATGCAGCTGTTCTTCAAGAACATGAAGACATACTTAGAAGCAAAGAATGTGGGGAGGCCCTCACTTGGGAAGATGTTTCCAAGATGAAGTACACATGGAGAGTAGCATTGGAGACTTTGAGACTTTTCCCACCTGTCTTTGGAGGGTTTAGAATGGCTCTCAAAGACATTCAATTAGGTGGATACACTATTCCAAAAGGATGGCAAATATTTTGGGCGGCATCAATGACCCATTTGGACGAGAAAATATTCAGAGACCCACAAAAGTTTGAACCAAGTAGATTTGATCAGAACCAAACGTCCATTCCACCACCCTTATCTTTCATCGCCTTTGGTGGAGGACCCAGAATTTGCCCTGGCTATGAGTTTGCAAAGCTTGAAACTCTTGTCACAATTCATTACATAATCACTCAGTTCACTTGGAAGCTCTCTTCACAAGACTTCTTGACCAGAGATCCAAGCTTGATGCCCAACAAAGGGTTACCCATTCAACTTTACCCCAAATTATGA

Protein sequence

MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPKL
BLAST of Cp4.1LG06g08860 vs. Swiss-Prot
Match: C16B1_PICSI (Cytochrome P450 716B1 OS=Picea sitchensis GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.8e-80
Identity = 164/406 (40.39%), Postives = 245/406 (60.34%), Query Frame = 1

Query: 2   TLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGAL 61
           +L G PTV       N+  +F  E + V N    S+  +     +T + G+D KR+R  L
Sbjct: 94  SLVGHPTVVFCSPEGNR-FLFSNENKLVVNSWPSSVGNLFRSSLITTV-GDDAKRLRRIL 153

Query: 62  VWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPT 121
           + FLRP++L+ FVG++D   ++HL  HW G  EV+  PL+K  TF + C L   I     
Sbjct: 154 MTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLFASINTKDD 213

Query: 122 RKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEE 181
              +   F   V G+  +PI+LP TRY+ +  A+  ++Q L  ++ ++   +E      E
Sbjct: 214 LDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIALEAGNASPE 273

Query: 182 DKNLITCLLSIKNKDKE-QALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYA 241
            ++L++ LLS  N D++ ++L++ EI  NI+LLL AGHDT+S  +T++L+ +A NP  Y 
Sbjct: 274 -QDLLSFLLS--NVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYE 333

Query: 242 AVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 301
            VL+E  +I  SKE G+ L WED+ +MKY+WRVA E LRLFP V G FR A+K+    G+
Sbjct: 334 EVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAIKEFIYDGF 393

Query: 302 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 361
           TIPKGW++ W  + TH   + F +P+KF+PSRF+      PPP +F+ FGGGPR+CPG E
Sbjct: 394 TIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEG---PPPYTFVPFGGGPRMCPGNE 453

Query: 362 FAKLETLVTIHYIITQFTWKL-SSQDFLTRDPSLMPNKGLPIQLYP 406
           FA++E L+ +H I+  F W L +  + +  DP   P  GLPI+L P
Sbjct: 454 FARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

BLAST of Cp4.1LG06g08860 vs. Swiss-Prot
Match: C16B2_PICSI (Cytochrome P450 716B2 OS=Picea sitchensis GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.5e-79
Identity = 164/406 (40.39%), Postives = 242/406 (59.61%), Query Frame = 1

Query: 2   TLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGAL 61
           +L G PTV       N+  +F  E + V N    S+  +     +T + G+D KR+R  L
Sbjct: 94  SLVGHPTVVFCSPEGNR-FLFSNENKLVVNSWPSSVGNLFRSSLITTV-GDDAKRLRRIL 153

Query: 62  VWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPT 121
           + FLRP++L+ FVG++D   ++HL  HW G  EV   PL+K  TF + C L   I     
Sbjct: 154 MTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLFASINNKDD 213

Query: 122 RKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEE 181
              +   F   V G+  +PI+LP TRY+ +  A+  ++Q L  ++ ++   +E      E
Sbjct: 214 LGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIGLEAGNASPE 273

Query: 182 DKNLITCLLSIKNKDKE-QALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYA 241
            ++L++ LLS  N D++ ++L++ EI  NI+LLL AGHDT+S  +T++L+ +A NP  Y 
Sbjct: 274 -QDLLSFLLS--NVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYE 333

Query: 242 AVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 301
            VL+E  DI  SKE G+ L WED+ +MKY+WRVA E LRLFP   G FR A+K+    G+
Sbjct: 334 EVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAIKEFIYDGF 393

Query: 302 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 361
           TIPKGW+++W  + TH   + F +P+ F+PSRF+      PPP +F+ FGGGPR+CPG E
Sbjct: 394 TIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEG---PPPYTFVPFGGGPRMCPGNE 453

Query: 362 FAKLETLVTIHYIITQFTWKL-SSQDFLTRDPSLMPNKGLPIQLYP 406
           FA+LE LV +H I+    W L +  + +  DP   P  GLPI+L P
Sbjct: 454 FARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVP 491

BLAST of Cp4.1LG06g08860 vs. Swiss-Prot
Match: T5H_TAXCU (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata PE=1 SV=2)

HSP 1 Score: 292.7 bits (748), Expect = 6.0e-78
Identity = 153/408 (37.50%), Postives = 251/408 (61.52%), Query Frame = 1

Query: 2   TLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGAL 61
           +L G PTV + G A N+ ++   EE+ V         +++GE ++    GEDH  +R AL
Sbjct: 95  SLIGHPTVVLCGPAGNR-LILSNEEKLVQMSWPAQFMKLMGENSVATRRGEDHIVMRSAL 154

Query: 62  VWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPT 121
             F  P +L+ ++GKM+ E++ H+N  W G  EV V PL++ L F+I   L F I +   
Sbjct: 155 AGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILFFNIYDKQE 214

Query: 122 RKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEE 181
           +  + +  +T++ G +++PI+LP   +  +L+  AK+ +I+  L+K + + + +      
Sbjct: 215 QDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKEDL-QSGSATA 274

Query: 182 DKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAA 241
            ++L++ LL+ ++ DK   L+ +EI+ N   LL A +DTT+  + L+ +++++NP  Y  
Sbjct: 275 TQDLLSVLLTFRD-DKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLSSNPECYQK 334

Query: 242 VLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGYT 301
           V+QE  +IL +KE GE +TW+D+  MKYTW+VA ETLR+FPPVFG FR A+ DIQ  GYT
Sbjct: 335 VVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAITDIQYDGYT 394

Query: 302 IPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEF 361
           IPKGW++ W    TH  +  F +P+KF PSRFDQ    +  P +F+ FGGG R C G+EF
Sbjct: 395 IPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHV-APYTFLPFGGGQRSCVGWEF 454

Query: 362 AKLETLVTIHYIITQFT--WKLSSQDFLTRDP-SLMPNKGLPIQLYPK 407
           +K+E L+ +H+ +  F+    +   + ++ DP   +P+KG  I+L+P+
Sbjct: 455 SKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498

BLAST of Cp4.1LG06g08860 vs. Swiss-Prot
Match: C7A52_PANGI (Beta-amyrin 28-oxidase OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.0e-77
Identity = 152/405 (37.53%), Postives = 241/405 (59.51%), Query Frame = 1

Query: 2   TLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGAL 61
           ++FG+P     G A NK  +F  E + V     +S+ ++      T  S E+  ++R  L
Sbjct: 81  SIFGEPAAVFCGAACNK-FLFSNENKLVQAWWPDSVNKVFPSSTQTS-SKEEAIKMRKML 140

Query: 62  VWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPT 121
             F +P++L+ ++G MD     H    W    EV+V PL K+ TF I C +   +EE   
Sbjct: 141 PNFFKPEALQRYIGLMDQIAANHFESGWENKNEVVVFPLAKSYTFWIACKVFVSVEEPAQ 200

Query: 122 RKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEE 181
              ++E F  +  GI SVPI+LP T ++ ++K+S  V++ L  ++K +  K++  E K  
Sbjct: 201 VAELLEPFSAIASGIISVPIDLPGTPFNSAIKSSKIVRRKLVGIIKQR--KIDLGEGKAS 260

Query: 182 DKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAA 241
               I   + + + +  + + E +I   I+ LLI GHDT S   T +++ +A  P +Y  
Sbjct: 261 ATQDILSHMLLTSDESGKFMGEGDIADKILGLLIGGHDTASSACTFVVKFLAELPQIYEG 320

Query: 242 VLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGYT 301
           V QE  +I++SK+ GE L WED+ KMKY+W VA E LRL PP+ G FR AL D    G++
Sbjct: 321 VYQEQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPLQGAFREALSDFTYNGFS 380

Query: 302 IPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEF 361
           IPKGW+++W+A+ TH++ ++F +P KF+PSRFD    + PPP SF+ FGGGPR+CPG E+
Sbjct: 381 IPKGWKLYWSANSTHINSEVFPEPLKFDPSRFD---GAGPPPFSFVPFGGGPRMCPGKEY 440

Query: 362 AKLETLVTIHYIITQFTW-KLSSQDFLTRDPSLMPNKGLPIQLYP 406
           A+LE LV +H+++ +F W K+   + +  +P  +P  GLP++L+P
Sbjct: 441 ARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFP 478

BLAST of Cp4.1LG06g08860 vs. Swiss-Prot
Match: T14H_TAXCU (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.4e-74
Identity = 147/408 (36.03%), Postives = 239/408 (58.58%), Query Frame = 1

Query: 2   TLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGAL 61
           +L G PTV + G A N+ ++   EE+ V     +S  +++GE+++T   GE H  +R AL
Sbjct: 93  SLIGHPTVVLCGPAGNR-LILANEEKLVQMSWPKSSMKLMGEKSITAKRGEGHMIIRSAL 152

Query: 62  VWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPT 121
             F  P +L+ ++G+M   +  H+N  W GN +V V  L+  L FDI   L F I E   
Sbjct: 153 QGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLVFDISACLFFNINEKHE 212

Query: 122 RKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEE 181
           R+ + E  + +  G+ +VP++LP   Y  +L+A +K+  IL  L++ +   +        
Sbjct: 213 RERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGLIEKRKMDLSSGLATS- 272

Query: 182 DKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAA 241
           +++L++  L+ K+ D+    S+EEI+ N   LL   +DTT   +  + +++++NP  Y  
Sbjct: 273 NQDLLSVFLTFKD-DRGNPCSDEEILDNFSGLLHGSYDTTVSAMACVFKLLSSNPECYEK 332

Query: 242 VLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGYT 301
           V+QE   IL +K  G+ +TW+DV  MKYTW+V  ETLRL+P +FG FR A+ DI   GY 
Sbjct: 333 VVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLYPSIFGSFRQAITDIHYNGYI 392

Query: 302 IPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEF 361
           IPKGW++ W    TH  E  F +P+KF PSRFDQ +  +  P +F+ FGGG R CPG+EF
Sbjct: 393 IPKGWKLLWTPYTTHPKEMYFSEPEKFLPSRFDQ-EGKLVAPYTFLPFGGGQRSCPGWEF 452

Query: 362 AKLETLVTIHYIITQFT--WKLSSQDFLTRDPSL-MPNKGLPIQLYPK 407
           +K+E L+++H+ +  F+    +   + + RD    +P+ G  ++L+P+
Sbjct: 453 SKMEILLSVHHFVKTFSTFTPVDPAEIIARDSLCPLPSNGFSVKLFPR 496

BLAST of Cp4.1LG06g08860 vs. TrEMBL
Match: A0A0A0L864_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G416660 PE=3 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 3.6e-202
Identity = 354/410 (86.34%), Postives = 381/410 (92.93%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           MTLFGKPTVF+HG AANKAVVF GEE  VSNRQ ESL+ ILG+RNLTELSGEDHKRVRGA
Sbjct: 72  MTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDHKRVRGA 131

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           LVWFLRPQ+L+ +VGKMDGEVR+HLN++WHGN EV VAPLMKTLTFDIICSLLFGIEEG 
Sbjct: 132 LVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLFGIEEGT 191

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEE-- 180
           TRKSIIECFKTMVDGIWS+PINLPFTRY+HSLKASAK QQILKQLLKDKA+ MEEE+E  
Sbjct: 192 TRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAKVMEEEKESE 251

Query: 181 -KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPT 240
            +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLMLRV+ATNPT
Sbjct: 252 VEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRVLATNPT 311

Query: 241 VYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQL 300
           VYAAVLQEHE+I RSKE GEALTWEDVSKMKYTWRVA+ETLRL+PPVFGGFR+ALKDIQL
Sbjct: 312 VYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRVALKDIQL 371

Query: 301 GGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICP 360
           G YTIPKGWQIFWAA MTHLDE IF DPQKFEPSRFDQNQT I PP SFIAFGGGPRICP
Sbjct: 372 GAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIAFGGGPRICP 431

Query: 361 GYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 407
           GYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 432 GYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480

BLAST of Cp4.1LG06g08860 vs. TrEMBL
Match: A0A0A0LA02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G186690 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.6e-149
Identity = 257/406 (63.30%), Postives = 325/406 (80.05%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           +TLFGKPTVF+ G + NK ++F G+   VSN+Q ESLR ILGERNL EL GEDHKRVR A
Sbjct: 70  LTLFGKPTVFIGGQSGNK-LIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKRVRNA 129

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           LV FL+P+ LK +VGKMD E+R H+ +HWH  +EV V PLMKTLTF+I+CSLLFG+E+G 
Sbjct: 130 LVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSLLFGLEQGT 189

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKE 180
            R+ +IECF+ M+ G+WS+PINLPFTRY+ S +AS K+Q++LK+LL +K  ++EE+    
Sbjct: 190 RRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVELEEKGGSS 249

Query: 181 EDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYA 240
             ++LITCLLSI+N++ E  LSE+EIVHNI+L+L+AG DT+S+LIT M+R +A NPTVYA
Sbjct: 250 H-QDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLANNPTVYA 309

Query: 241 AVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 300
           AVLQE E+I RSK+CGE L WED++KMKYTWRVALETLRL  P+FGGFR A+ DIQ GGY
Sbjct: 310 AVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGY 369

Query: 301 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 360
            IPKGWQIFW + +THLD+ IFR+P KF+P RF +N  SI PP  F+ FG GPRICPG E
Sbjct: 370 LIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRF-KNPASI-PPYCFLGFGSGPRICPGNE 429

Query: 361 FAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPK 407
           FA++ETLVTIHY+ITQFTWKL   D   RDP   P KG+PI+++P+
Sbjct: 430 FARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPR 471

BLAST of Cp4.1LG06g08860 vs. TrEMBL
Match: M5WNT9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023488mg PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 1.9e-142
Identity = 241/406 (59.36%), Postives = 326/406 (80.30%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           ++LFGKPTVF+HG AANK  VF  + + ++++Q ES R ILG+RN+ ELSGEDHKRVR A
Sbjct: 71  LSLFGKPTVFIHGQAANK-YVFNNDGKTITSQQPESTRMILGDRNILELSGEDHKRVRNA 130

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           L+ FL+P+SLK +VGK+D E+RKHL L+WHG +++ V PLMK LTF+IICSLLFG+E GP
Sbjct: 131 LMVFLKPESLKQYVGKLDEEIRKHLELNWHGKQKITVLPLMKNLTFNIICSLLFGVERGP 190

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKE 180
            R  +IECF+ M++G+WSVP+NLPFTR++ S+KAS KVQ +LK+L+ +K  ++E++    
Sbjct: 191 RRDELIECFQRMIEGLWSVPLNLPFTRFNCSIKASKKVQNMLKELICEKRMQLEQKTALP 250

Query: 181 EDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYA 240
             ++LITCLLSI+N D E+ L+E+EI+HNII++++AG+DT+S++IT +LR++A  P VYA
Sbjct: 251 L-QDLITCLLSIRNADNEEELTEKEILHNIIIVMVAGYDTSSVVITFLLRLLANEPAVYA 310

Query: 241 AVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 300
           A+LQE E+I RSK  GE LTWED++KMKYTWRV +E LR  PPVFGG R A+KDI+ GG+
Sbjct: 311 ALLQEQEEIARSKSLGELLTWEDLAKMKYTWRVTMEILRTTPPVFGGMRRAMKDIEYGGF 370

Query: 301 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 360
            IP+GWQIFWA  MTH D+ IF +P KF+PSRF +NQ S+ PP SF+ FGGG RICPGYE
Sbjct: 371 LIPQGWQIFWAIPMTHNDDSIFPEPSKFDPSRF-ENQKSV-PPYSFVPFGGGTRICPGYE 430

Query: 361 FAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPK 407
           FA++E LV IHY++TQFTWKL + +  +R P  +P +GLPI++ P+
Sbjct: 431 FARIEILVAIHYMVTQFTWKLCADNKFSRVPMPVPTQGLPIEITPR 472

BLAST of Cp4.1LG06g08860 vs. TrEMBL
Match: A0A061FD55_THECC (Cytochrome P450 OS=Theobroma cacao GN=TCM_030948 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.2e-142
Identity = 241/407 (59.21%), Postives = 325/407 (79.85%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           M+LFGKP+VF++G  ANK  VF  +   + N+Q +S+  ILG+R + EL GEDHKRVR A
Sbjct: 70  MSLFGKPSVFIYGQTANK-FVFTSDSSTIVNQQVKSITAILGDRCILELIGEDHKRVRDA 129

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           LV FL+P+SLK FVGKMD EVRKHL +HWHG ++V V PLMKTLTF+IICSLLFG+E G 
Sbjct: 130 LVSFLKPESLKEFVGKMDEEVRKHLEMHWHGKQQVKVLPLMKTLTFNIICSLLFGLERGT 189

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKE 180
            R  +++ F++M++G+WSVP+NLPFTRY+ SL+ASA+ Q++LK L+ +K   +E++    
Sbjct: 190 RRDKLVDDFQSMIEGMWSVPVNLPFTRYNRSLQASARAQKMLKGLIGEKRVDLEQKGASP 249

Query: 181 EDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYA 240
             ++LITCLLSI+N+  E  +SE+EI+HN++L+++AGHDT+S+L+T ++R++A +P VYA
Sbjct: 250 R-QDLITCLLSIRNERNEAVISEKEIIHNVMLIMVAGHDTSSVLLTFLVRLLANDPAVYA 309

Query: 241 AVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 300
           AVLQE E+I +SK  GE LTWED++KMKYTW+VA+ETLRLFPP+FGGFR A+KDI+ GGY
Sbjct: 310 AVLQEQEEIAKSKPDGELLTWEDLAKMKYTWKVAMETLRLFPPIFGGFRKAVKDIEYGGY 369

Query: 301 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 360
            IPK WQIFW   +TH+D+ IF +P KF+P+RF+ N  SIPP   FI FGGGPRICPGYE
Sbjct: 370 LIPKDWQIFWVTGITHMDDSIFLEPSKFDPTRFE-NPASIPP-YCFIPFGGGPRICPGYE 429

Query: 361 FAKLETLVTIHYIITQFTWK-LSSQDFLTRDPSLMPNKGLPIQLYPK 407
           FA++ETLV++HY++T+FTW  L S +F +RDP   P KGLP+QL P+
Sbjct: 430 FARVETLVSVHYLVTRFTWTLLCSDNFFSRDPMPAPTKGLPVQLSPR 472

BLAST of Cp4.1LG06g08860 vs. TrEMBL
Match: A0A151U234_CAJCA (Cytochrome P450 716B1 family OS=Cajanus cajan GN=KK1_006013 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.7e-141
Identity = 235/406 (57.88%), Postives = 323/406 (79.56%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           ++LFGKPTV ++G AANK  +F G    + N+QT+S++ ILG+RNL EL GEDH RVRGA
Sbjct: 23  LSLFGKPTVLIYGQAANK-FIFSGGGNTIVNQQTQSVKMILGDRNLLELCGEDHSRVRGA 82

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           LV FL+P+SLK +VGKMD EVR+HL +HW G +++ V PLMKTLTF+IICSLLFG+E G 
Sbjct: 83  LVSFLKPESLKQYVGKMDEEVRRHLEMHWQGEQQIKVLPLMKTLTFNIICSLLFGLERGK 142

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKE 180
            R   ++ F+ M++G+WSVPIN+PFTRY+ SL+ASA++Q +LK+++  K  ++++     
Sbjct: 143 QRDQFLDSFQAMIEGMWSVPINVPFTRYNRSLRASARIQNLLKEIVHKKKTELKQNGASA 202

Query: 181 EDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYA 240
            D++LI+CLL + ++D +Q +++ EI+HN+ L+++AGHDT+S+LIT ++R++A  P + A
Sbjct: 203 -DQDLISCLLGMVDEDGKQVITDMEIIHNVSLVMVAGHDTSSVLITFIIRLLANEPAICA 262

Query: 241 AVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 300
           AVLQE E+I +SK  GEALTWED+SKMKYTW+VA+ETLR+FPP+FGGFR A  DI+  GY
Sbjct: 263 AVLQEQEEITKSKPSGEALTWEDLSKMKYTWKVAMETLRMFPPIFGGFRKAATDIEYDGY 322

Query: 301 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 360
            IPKGWQIFW  +MTH+D+ IF +P KFEPSRF +NQ S  PP  FI FGGG RICPGYE
Sbjct: 323 LIPKGWQIFWVTAMTHMDKSIFPEPSKFEPSRF-ENQAS-APPYCFIPFGGGTRICPGYE 382

Query: 361 FAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPK 407
           FA+LETLV IHY++T+F+WKL S +F +RDP  +P +GLP+Q++P+
Sbjct: 383 FARLETLVAIHYLVTRFSWKLCSDNFFSRDPMPVPTQGLPVQIWPR 424

BLAST of Cp4.1LG06g08860 vs. TAIR10
Match: AT5G36110.1 (AT5G36110.1 cytochrome P450, family 716, subfamily A, polypeptide 1)

HSP 1 Score: 289.7 bits (740), Expect = 2.9e-78
Identity = 148/406 (36.45%), Postives = 248/406 (61.08%), Query Frame = 1

Query: 3   LFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALV 62
           LFG PT  V G + NK  +F  E + V +   +S+ +I      T  S E+ +++R  L 
Sbjct: 82  LFGSPTAVVTGASGNK-FLFTNENKLVVSWWPDSVNKIFPSSMQTS-SKEEARKLRMLLS 141

Query: 63  WFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPTR 122
            F++P++L+ +VG MD   ++H    W    +VIV PL K  TF I C     +E+    
Sbjct: 142 QFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFTFSIACRSFLSMEDPARV 201

Query: 123 KSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEED 182
           + + E F T+  GI+S+PI+LP TR++ ++KAS  +++ +  +++ + ++++  +  EE 
Sbjct: 202 RQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAIVRQRKEELKAGKALEEH 261

Query: 183 KNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAAV 242
             L   L++I          +E++   II LLI GHDT SI+ T ++  +A  P VY  V
Sbjct: 262 DILSHMLMNIGET------KDEDLADKIIGLLIGGHDTASIVCTFVVNYLAEFPHVYQRV 321

Query: 243 LQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGYTI 302
           LQE ++IL+ K+  E L WED+ KM+Y+W VA E +R+ PP+ G FR A+      G+ I
Sbjct: 322 LQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFREAIDHFSFKGFYI 381

Query: 303 PKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEFA 362
           PKGW+++W+A+ TH++   F +P++FEP+RF   + S P P +++ FGGGPR+CPG E+A
Sbjct: 382 PKGWKLYWSATATHMNPDYFPEPERFEPNRF---EGSGPKPYTYVPFGGGPRMCPGKEYA 441

Query: 363 KLETLVTIHYIITQFTWK--LSSQDFLTRDPSLMPNKGLPIQLYPK 407
           +LE L+ +H ++ +F W+    +++ +  DP  +P+KGLPI+++P+
Sbjct: 442 RLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQ 476

BLAST of Cp4.1LG06g08860 vs. TAIR10
Match: AT2G42850.1 (AT2G42850.1 cytochrome P450, family 718)

HSP 1 Score: 276.6 bits (706), Expect = 2.5e-74
Identity = 142/406 (34.98%), Postives = 245/406 (60.34%), Query Frame = 1

Query: 3   LFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALV 62
           + G PT+ V+G  AN+ ++       VS+  + S++ ++G   +    GE H+ +RG + 
Sbjct: 90  IMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQ-LMGMNCIMAKQGEKHRVLRGIVA 149

Query: 63  WFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPTR 122
             L    L+  + K+   V+ H    W G +E+ +    K LTF ++   L+GI+     
Sbjct: 150 NSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYGIK---VE 209

Query: 123 KSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEED 182
             ++E F+ +++G++++P+  P ++++ + KA  +++  L   +++K ++ME+E  ++ +
Sbjct: 210 IGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRREMEKEGAEKPN 269

Query: 183 KNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAAV 242
             L + L+    K     ++EEE+V N++LL+ A HDTTS  +++  +++A +PT    +
Sbjct: 270 TTLFSRLVEELIKG---VITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTL 329

Query: 243 LQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGYTI 302
           LQEH  I  +K  GE LT EDV KMKY+W+V  ET+RL PP+FG FR A+ DI  GGYTI
Sbjct: 330 LQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTI 389

Query: 303 PKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEFA 362
           PKGW+I W    TH + +IF+DP  F+P+RFD+   +     +++ FGGGPR+C G++ A
Sbjct: 390 PKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDKPIQA----YTYLPFGGGPRLCAGHQLA 449

Query: 363 KLETLVTIHYIITQFTWKLSSQD-FLTRDPSLMPNKGLPIQLYPKL 408
           K+  LV +H+++T F W L   D  ++ DP   P+ G+PI++ PK+
Sbjct: 450 KISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPKV 484

BLAST of Cp4.1LG06g08860 vs. TAIR10
Match: AT5G05690.1 (AT5G05690.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 212.2 bits (539), Expect = 5.9e-55
Identity = 128/400 (32.00%), Postives = 212/400 (53.00%), Query Frame = 1

Query: 3   LFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALV 62
           LFG+PT+F      N+ V+   E +        S+  +LG+ +L  + G  HKR+    +
Sbjct: 75  LFGEPTIFSADPETNRFVL-QNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTM 134

Query: 63  WFLRPQSLKGFVG-KMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPT 122
            F     +K  +   +D  VR +L+  W  +  V++    K +TF++    L   + G  
Sbjct: 135 SFANSSIIKDHLMLDIDRLVRFNLD-SW--SSRVLLMEEAKKITFELTVKQLMSFDPGEW 194

Query: 123 RKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEE 182
            +S+ + +  +++G +S+P+ L  T Y  +++A  KV + L  ++  + +  EEEE  E 
Sbjct: 195 SESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRRE--EEEEGAER 254

Query: 183 DKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAA 242
            K+++  LL+      +   S+EEIV  ++ LL+AG++TTS ++TL ++ +   P   A 
Sbjct: 255 KKDMLAALLAA-----DDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQ 314

Query: 243 VLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGYT 302
           + +EHE I   K    +L W D   M +T  V  ETLR+   + G FR A+ D+++ GY 
Sbjct: 315 LKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYK 374

Query: 303 IPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEF 362
           IPKGW++F +    HLD   F+D + F P R+  N  +  P   F  FGGGPR+CPGYE 
Sbjct: 375 IPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYEL 434

Query: 363 AKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPI 402
           A++   V +H ++T F+W  + QD L   P+    K  PI
Sbjct: 435 ARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPI 463

BLAST of Cp4.1LG06g08860 vs. TAIR10
Match: AT5G45340.1 (AT5G45340.1 cytochrome P450, family 707, subfamily A, polypeptide 3)

HSP 1 Score: 210.3 bits (534), Expect = 2.2e-54
Identity = 136/405 (33.58%), Postives = 211/405 (52.10%), Query Frame = 1

Query: 3   LFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALV 62
           + G P V +    A K V+ + +          S  R+LG++ +    G+ H ++R  ++
Sbjct: 76  VLGCPCVMISSPEAAKFVL-VTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVL 135

Query: 63  WFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPTR 122
               P +++  V  ++   ++ LN  W G  ++     MKT TF++    + G +E   R
Sbjct: 136 RAFMPDAIRNMVPHIESIAQESLN-SWDGT-QLNTYQEMKTYTFNVALISILGKDEVYYR 195

Query: 123 KSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEED 182
           + +  C+  +  G  S+PINLP T +  ++KA  ++ QIL  +L  + Q           
Sbjct: 196 EDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSSH------ 255

Query: 183 KNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAAV 242
               T LL    +DK   L++E+I  NII ++ A  DTT+ ++T +L+ +A NPTV  AV
Sbjct: 256 ----TDLLGSFMEDKA-GLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAV 315

Query: 243 LQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGYTI 302
            +E   I + K+ GE+LTWED  KM  T+RV  ETLR    +   FR A++D++  GY I
Sbjct: 316 TEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLI 375

Query: 303 PKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEFA 362
           PKGW++       H +  IF DP KF+PSRF+      P P +F+ FG G   CPG E A
Sbjct: 376 PKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE----VAPKPNTFMPFGSGIHSCPGNELA 435

Query: 363 KLETLVTIHYIITQFTWKL-SSQDFLTRDPSLMPNKGLPIQLYPK 407
           KLE  V IH++ T++ W +    D +   P  +P  GLPI L  K
Sbjct: 436 KLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIALERK 462

BLAST of Cp4.1LG06g08860 vs. TAIR10
Match: AT3G13730.1 (AT3G13730.1 cytochrome P450, family 90, subfamily D, polypeptide 1)

HSP 1 Score: 205.7 bits (522), Expect = 5.5e-53
Identity = 118/401 (29.43%), Postives = 217/401 (54.11%), Query Frame = 1

Query: 3   LFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALV 62
           +FG  T+       N+AV+   +  A      +++R ++G+ ++  ++G  H+R  G + 
Sbjct: 95  IFGTATIVSTDAEVNRAVL-QSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVG 154

Query: 63  WFLRPQSLKG-FVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPT 122
            FL+   LK   V  M   + + ++L W  ++ V++  + KT+ F ++   L  +E+G  
Sbjct: 155 SFLKSPLLKAQIVRDMHKFLSESMDL-WSEDQPVLLQDVSKTVAFKVLAKALISVEKGED 214

Query: 123 RKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEE 182
            + +   F+  + G+ S+PIN P T+   SL+A   + + ++++++ K +K +    KEE
Sbjct: 215 LEELKREFENFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKN---KEE 274

Query: 183 DKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAA 242
           D  +   ++ +  KD  + L+   I +N+I ++I GHD+  +LITL ++ ++ +P     
Sbjct: 275 DDVIAKDVVDVLLKDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNL 334

Query: 243 VLQEHEDILRSKEC-GEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 302
           + +E+  +   KE  GE L W D   + +T +V  ETLR+   + G  R A+KD+++ GY
Sbjct: 335 LTEENMKLKSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGY 394

Query: 303 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 362
            IPKGW         HLD+  +  P KF P R+ +   +     SF  FGGG R+CPG +
Sbjct: 395 VIPKGWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTS---SFSPFGGGQRLCPGLD 454

Query: 363 FAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPI 402
            A+LET V +H+++T+F W ++ +D +   P++     LPI
Sbjct: 455 LARLETSVFLHHLVTRFRW-IAEEDTIINFPTVHMKNKLPI 486

BLAST of Cp4.1LG06g08860 vs. NCBI nr
Match: gi|449468638|ref|XP_004152028.1| (PREDICTED: taxadiene 5-alpha hydroxylase-like [Cucumis sativus])

HSP 1 Score: 712.2 bits (1837), Expect = 5.1e-202
Identity = 354/410 (86.34%), Postives = 381/410 (92.93%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           MTLFGKPTVF+HG AANKAVVF GEE  VSNRQ ESL+ ILG+RNLTELSGEDHKRVRGA
Sbjct: 72  MTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDHKRVRGA 131

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           LVWFLRPQ+L+ +VGKMDGEVR+HLN++WHGN EV VAPLMKTLTFDIICSLLFGIEEG 
Sbjct: 132 LVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLFGIEEGT 191

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEE-- 180
           TRKSIIECFKTMVDGIWS+PINLPFTRY+HSLKASAK QQILKQLLKDKA+ MEEE+E  
Sbjct: 192 TRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAKVMEEEKESE 251

Query: 181 -KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPT 240
            +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLMLRV+ATNPT
Sbjct: 252 VEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRVLATNPT 311

Query: 241 VYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQL 300
           VYAAVLQEHE+I RSKE GEALTWEDVSKMKYTWRVA+ETLRL+PPVFGGFR+ALKDIQL
Sbjct: 312 VYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRVALKDIQL 371

Query: 301 GGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICP 360
           G YTIPKGWQIFWAA MTHLDE IF DPQKFEPSRFDQNQT I PP SFIAFGGGPRICP
Sbjct: 372 GAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIAFGGGPRICP 431

Query: 361 GYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 407
           GYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 432 GYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480

BLAST of Cp4.1LG06g08860 vs. NCBI nr
Match: gi|659109112|ref|XP_008454553.1| (PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like [Cucumis melo])

HSP 1 Score: 694.1 bits (1790), Expect = 1.4e-196
Identity = 345/408 (84.56%), Postives = 379/408 (92.89%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           MTLFGKPTVFVHG AANKAVVF GEE  VSNRQ ESL+ ILG+RNLTELSGEDHKRVRGA
Sbjct: 72  MTLFGKPTVFVHGAAANKAVVFSGEEGTVSNRQIESLKMILGKRNLTELSGEDHKRVRGA 131

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           LVWFLRPQ+L+ +V KMDGEVR+HLN++WHGN +V VAPLMKTLTFDIICSLLFGIEEG 
Sbjct: 132 LVWFLRPQTLRSYVEKMDGEVRRHLNMYWHGNNKVTVAPLMKTLTFDIICSLLFGIEEGT 191

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEE-K 180
           TRKSII+CFKTMV+GIWSVPINLPFTRY+HSLKASAKVQQILKQLLKDKA++ME++++ K
Sbjct: 192 TRKSIIQCFKTMVEGIWSVPINLPFTRYNHSLKASAKVQQILKQLLKDKAKRMEQKKKMK 251

Query: 181 EEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVY 240
           ++ K+LIT LL IKNKDKEQAL+EEEI+HNIILL+IAGHDTTSIL+TLMLRV+ATNPTVY
Sbjct: 252 KKMKDLITYLLRIKNKDKEQALTEEEILHNIILLMIAGHDTTSILLTLMLRVLATNPTVY 311

Query: 241 AAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGG 300
           A VLQEHE+I RSKE GEALTWEDVSKMKYTWRVA+ETLRL+PPVFGGFR+ALKDIQLG 
Sbjct: 312 ADVLQEHEEIARSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRVALKDIQLGA 371

Query: 301 YTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGY 360
           YTIPKGWQIFWAAS THLDE IF DPQKFEPSRFDQ QT I PP SFIAFGGGPRICPGY
Sbjct: 372 YTIPKGWQIFWAASTTHLDETIFGDPQKFEPSRFDQTQTPI-PPFSFIAFGGGPRICPGY 431

Query: 361 EFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 407
           EFAKLETLVTIHY+ITQFTW LS SQD LTRDP+LMPNKGLPIQ++PK
Sbjct: 432 EFAKLETLVTIHYLITQFTWNLSCSQDSLTRDPTLMPNKGLPIQIFPK 478

BLAST of Cp4.1LG06g08860 vs. NCBI nr
Match: gi|659114553|ref|XP_008457111.1| (PREDICTED: cytochrome P450 716B1-like [Cucumis melo])

HSP 1 Score: 541.2 bits (1393), Expect = 1.6e-150
Identity = 257/406 (63.30%), Postives = 328/406 (80.79%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           +TLFGKPTVF+ G + NK ++F G+   VSN+Q ESLR ILGERNL EL+GEDHKRVR A
Sbjct: 70  LTLFGKPTVFIGGQSGNK-LIFSGDCAVVSNQQNESLRAILGERNLLELTGEDHKRVRNA 129

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           LV FL+P+ LK +VGKMD E+R H+++HWH  +EV V PLMKTLTF+I+CSLLFG+E+G 
Sbjct: 130 LVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSLLFGLEQGM 189

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKE 180
            R+ +IECF+ M+ G+WS+PINLPFTRY+ S +AS K+Q++LK+LL +K  ++EE+    
Sbjct: 190 RRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVELEEKGGSS 249

Query: 181 EDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYA 240
             ++LITCLLSI+N++ E  LSE+EIVHNI+L+++AG DT+S+LIT M+R +A NPTVYA
Sbjct: 250 H-QDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLANNPTVYA 309

Query: 241 AVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 300
           AVLQE E+I RSK+CGE L WED++KMKYTWRVALETLRL  P+FGGFR A+ DIQ GGY
Sbjct: 310 AVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGY 369

Query: 301 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 360
            IPKGWQIFW + +THLD+ IFR+P KF+P RF +N  SI PP  F+ FG GPRICPG E
Sbjct: 370 LIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRF-KNPASI-PPYCFLGFGSGPRICPGNE 429

Query: 361 FAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPK 407
           FA++ETLVTIHY+ITQFTWKL S D   RDP   P KG+PI+++P+
Sbjct: 430 FARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR 471

BLAST of Cp4.1LG06g08860 vs. NCBI nr
Match: gi|449446129|ref|XP_004140824.1| (PREDICTED: cytochrome P450 716B1-like [Cucumis sativus])

HSP 1 Score: 537.3 bits (1383), Expect = 2.3e-149
Identity = 257/406 (63.30%), Postives = 325/406 (80.05%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           +TLFGKPTVF+ G + NK ++F G+   VSN+Q ESLR ILGERNL EL GEDHKRVR A
Sbjct: 70  LTLFGKPTVFIGGQSGNK-LIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKRVRNA 129

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           LV FL+P+ LK +VGKMD E+R H+ +HWH  +EV V PLMKTLTF+I+CSLLFG+E+G 
Sbjct: 130 LVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSLLFGLEQGT 189

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKE 180
            R+ +IECF+ M+ G+WS+PINLPFTRY+ S +AS K+Q++LK+LL +K  ++EE+    
Sbjct: 190 RRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVELEEKGGSS 249

Query: 181 EDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYA 240
             ++LITCLLSI+N++ E  LSE+EIVHNI+L+L+AG DT+S+LIT M+R +A NPTVYA
Sbjct: 250 H-QDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLANNPTVYA 309

Query: 241 AVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 300
           AVLQE E+I RSK+CGE L WED++KMKYTWRVALETLRL  P+FGGFR A+ DIQ GGY
Sbjct: 310 AVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGY 369

Query: 301 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 360
            IPKGWQIFW + +THLD+ IFR+P KF+P RF +N  SI PP  F+ FG GPRICPG E
Sbjct: 370 LIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRF-KNPASI-PPYCFLGFGSGPRICPGNE 429

Query: 361 FAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPK 407
           FA++ETLVTIHY+ITQFTWKL   D   RDP   P KG+PI+++P+
Sbjct: 430 FARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPR 471

BLAST of Cp4.1LG06g08860 vs. NCBI nr
Match: gi|470116394|ref|XP_004294367.1| (PREDICTED: cytochrome P450 716B1-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 526.9 bits (1356), Expect = 3.0e-146
Identity = 247/406 (60.84%), Postives = 332/406 (81.77%), Query Frame = 1

Query: 1   MTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGA 60
           ++LFGKPTVF+HG AANK  VF  +   +S++QTES++RILGER++ ELS EDHKRVR A
Sbjct: 71  LSLFGKPTVFIHGQAANK-FVFTKDGSTISSQQTESIKRILGERSIFELSIEDHKRVRSA 130

Query: 61  LVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGP 120
           L+ FL+P+SLK +VGKMD E+RKHL +HWHG K+V V PLMK LTF+IICSLLFG+E G 
Sbjct: 131 LMLFLKPESLKQYVGKMDEEIRKHLEMHWHG-KQVTVLPLMKNLTFNIICSLLFGLERGT 190

Query: 121 TRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKE 180
            R+  +ECF+TM++G+WSVPINLPFTRY+ SLKAS +VQ +LK+L+ +K  ++E+++   
Sbjct: 191 RREKFVECFQTMIEGMWSVPINLPFTRYNRSLKASKRVQNMLKELIHEKRIQLEQKKASP 250

Query: 181 EDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYA 240
           + ++LITC LSI+N++ E+ L+E E+VHN++++++AGHDT+S+LIT +LR++A  P VY 
Sbjct: 251 Q-QDLITCFLSIRNEEGEEELTELEMVHNVMVVMVAGHDTSSVLITFLLRLLANEPVVYE 310

Query: 241 AVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGY 300
           A+LQE E++ RSK  GE LTWED++KMKYTWRVA ETLR+ PPVFGGFR A+KDI+ GG+
Sbjct: 311 ALLQEQEEVARSKPAGEFLTWEDLAKMKYTWRVAQETLRITPPVFGGFRTAIKDIEFGGF 370

Query: 301 TIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYE 360
            IPKGWQIFW A MTH+D+ IF +P KF+PSRFD+ Q S+ PP  F+AFGGGPRICPGYE
Sbjct: 371 NIPKGWQIFWVAPMTHMDDSIFPEPAKFDPSRFDK-QASV-PPYCFVAFGGGPRICPGYE 430

Query: 361 FAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPK 407
           FA++ETL+ +H+IITQFTWKL + +  +R P  +P  GLPI++ P+
Sbjct: 431 FARIETLIAMHHIITQFTWKLCADNHFSRAPMPVPTGGLPIEIKPR 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C16B1_PICSI3.8e-8040.39Cytochrome P450 716B1 OS=Picea sitchensis GN=CYP716B1 PE=2 SV=1[more]
C16B2_PICSI2.5e-7940.39Cytochrome P450 716B2 OS=Picea sitchensis GN=CYP716B2 PE=2 SV=1[more]
T5H_TAXCU6.0e-7837.50Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata PE=1 SV=2[more]
C7A52_PANGI3.0e-7737.53Beta-amyrin 28-oxidase OS=Panax ginseng PE=2 SV=1[more]
T14H_TAXCU1.4e-7436.03Taxoid 14-beta-hydroxylase OS=Taxus cuspidata PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L864_CUCSA3.6e-20286.34Uncharacterized protein OS=Cucumis sativus GN=Csa_3G416660 PE=3 SV=1[more]
A0A0A0LA02_CUCSA1.6e-14963.30Uncharacterized protein OS=Cucumis sativus GN=Csa_3G186690 PE=3 SV=1[more]
M5WNT9_PRUPE1.9e-14259.36Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023488mg PE=3 SV=1[more]
A0A061FD55_THECC3.2e-14259.21Cytochrome P450 OS=Theobroma cacao GN=TCM_030948 PE=3 SV=1[more]
A0A151U234_CAJCA2.7e-14157.88Cytochrome P450 716B1 family OS=Cajanus cajan GN=KK1_006013 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G36110.12.9e-7836.45 cytochrome P450, family 716, subfamily A, polypeptide 1[more]
AT2G42850.12.5e-7434.98 cytochrome P450, family 718[more]
AT5G05690.15.9e-5532.00 Cytochrome P450 superfamily protein[more]
AT5G45340.12.2e-5433.58 cytochrome P450, family 707, subfamily A, polypeptide 3[more]
AT3G13730.15.5e-5329.43 cytochrome P450, family 90, subfamily D, polypeptide 1[more]
Match NameE-valueIdentityDescription
gi|449468638|ref|XP_004152028.1|5.1e-20286.34PREDICTED: taxadiene 5-alpha hydroxylase-like [Cucumis sativus][more]
gi|659109112|ref|XP_008454553.1|1.4e-19684.56PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like [Cucumis melo... [more]
gi|659114553|ref|XP_008457111.1|1.6e-15063.30PREDICTED: cytochrome P450 716B1-like [Cucumis melo][more]
gi|449446129|ref|XP_004140824.1|2.3e-14963.30PREDICTED: cytochrome P450 716B1-like [Cucumis sativus][more]
gi|470116394|ref|XP_004294367.1|3.0e-14660.84PREDICTED: cytochrome P450 716B1-like [Fragaria vesca subsp. vesca][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0010295 (+)-abscisic acid 8'-hydroxylase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG06g08860.1Cp4.1LG06g08860.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 272..283
score: 1.5E-7coord: 347..356
score: 1.5E-7coord: 356..367
score: 1.5E-7coord: 216..233
score: 1.
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 2..405
score: 3.3
IPR001128Cytochrome P450PFAMPF00067p450coord: 4..396
score: 2.4
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 2..405
score: 2.36
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 205..222
score: 1.1E-14coord: 104..122
score: 1.1E-14coord: 356..379
score: 1.1E-14coord: 225..251
score: 1.1E-14coord: 346..356
score: 1.1E-14coord: 311..335
score: 1.1
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 349..358
scor
NoneNo IPR availableunknownCoilCoilcoord: 156..183
scor
NoneNo IPR availablePANTHERPTHR24286FAMILY NOT NAMEDcoord: 1..406
score: 2.3E
NoneNo IPR availablePANTHERPTHR24286:SF61SUBFAMILY NOT NAMEDcoord: 1..406
score: 2.3E

The following gene(s) are paralogous to this gene:

None