Cp4.1LG05g13540 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG05g13540 vs. Swiss-Prot
Match: SYAP_ORYSI (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica GN=OsI_22356 PE=3 SV=1)

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 644/936 (68.80%), Postives = 757/936 (80.88%), Query Frame = 1

Query: 101 QTFSSKRSSGHTTQNKKYITSVKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVL 160
           ++  S R  G   +      SV+   +E+    S +   SGD+IRRRFL FYA+RGHK+L
Sbjct: 63  RSMGSSRERGVLVKTSSSSASVESATQEVGAASSGEW--SGDAIRRRFLDFYAARGHKIL 122

Query: 161 PSASLVPEDPTVLLTIAGMLQFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHT 220
           PS+SLVP+DPTV LTIAGMLQFKPIFLGK PR VPCA T+Q+CIRTND+ENVGRT+RH T
Sbjct: 123 PSSSLVPDDPTVFLTIAGMLQFKPIFLGKEPRRVPCATTSQKCIRTNDIENVGRTSRHQT 182

Query: 221 FFEMLGNFSFGDYFKKEAIKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPV 280
           FFEMLGNFSFGDYFKKEAI WAWELTT EFGLP  RLWIS++++DDEAF+IWH+EVGVP 
Sbjct: 183 FFEMLGNFSFGDYFKKEAITWAWELTTKEFGLPPERLWISVFQDDDEAFSIWHNEVGVPK 242

Query: 281 ERIKRMGEDDNFWTSGITGPCGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYN 340
           ERIKR+GEDDNFWTSG TGPCGPCSEIYYDF PE+G SD DLGDD+RF+EFYNLVFMQYN
Sbjct: 243 ERIKRLGEDDNFWTSGATGPCGPCSEIYYDFYPERGSSDADLGDDSRFIEFYNLVFMQYN 302

Query: 341 KKDDGSLEPLKQKNIDTGLGLERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADN 400
           KKDDGSLEPLKQKNIDTG+GLERMARILQKVPNNYETDLI+PII+KA+ +A V+Y TAD+
Sbjct: 303 KKDDGSLEPLKQKNIDTGMGLERMARILQKVPNNYETDLIFPIIEKAASMALVSYTTADD 362

Query: 401 QSKTSLKIIGDHMRAIVYLISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTD 460
             KT+LKIIGDHMRA+VYLISDGV+PSNIGRGY+VRRLIRRVVRTGRL+GI+GDG GN++
Sbjct: 363 AMKTNLKIIGDHMRAVVYLISDGVIPSNIGRGYVVRRLIRRVVRTGRLIGIRGDGHGNSE 422

Query: 461 GAFTPHIAEKVIALSSHIDEDVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALA 520
           GAF P +AE  I+LS+ ID DV+ R   IL EL+REELRFVQTLERGEKLL+E+L +AL+
Sbjct: 423 GAFLPSLAEVAISLSTEIDPDVESRRKSILGELQREELRFVQTLERGEKLLDELLDEALS 482

Query: 521 SAKEDGRFPCLAGKDAFLLYDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAA 580
           SA  +G  PCL+GKD FLLYDTYGFPVEIT E+A ERGV+VDMKGFD+EM+NQR+QSQAA
Sbjct: 483 SAGNNGGKPCLSGKDVFLLYDTYGFPVEITAEIAGERGVIVDMKGFDMEMENQRKQSQAA 542

Query: 581 HNAVKLEVGEGADIMENISDTEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNR 640
           HN VKL VG   +I+++I DTEFLGYD LSA A+V+ L+VNG SV  VSEG DVE+ L+R
Sbjct: 543 HNVVKLSVGNETEIVKSIPDTEFLGYDSLSATAVVKGLLVNGNSVNVVSEGSDVEIFLDR 602

Query: 641 TPFYAESGGQIGDHGFIYI-SEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGRE 700
           TPFYAESGGQ+GD+GF+Y+  E + +   V+E+ DVQKS G+IFVHKGTIKQG +EVG+E
Sbjct: 603 TPFYAESGGQVGDNGFLYVYGEEDAKQKAVIEINDVQKSLGNIFVHKGTIKQGSVEVGKE 662

Query: 701 VKAEVDAKLRQGAKVTLFAC------------QETSQAGSLVAFDRLRFDFNYHRPLADT 760
           + A VDAKLRQGAK    A              ETSQAGSLVAFDRLRFDFN+HRPL++ 
Sbjct: 663 IDAAVDAKLRQGAKAHHTATHLLQSALKSIIGSETSQAGSLVAFDRLRFDFNFHRPLSEE 722

Query: 761 EILQIEELINGWIGDAVLLQTKVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCG 820
           E+++IE L+N W+  A  L+TKVM L +AK+AGAIAMFGEKYGE+VRVVEVPGVSMELCG
Sbjct: 723 ELMKIESLVNQWVSSATHLETKVMDLQDAKNAGAIAMFGEKYGEQVRVVEVPGVSMELCG 782

Query: 821 GTHVFNTSEIRGFKVISEQGIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAED 880
           GTHV NT+EIRGFK+ISEQGIASGVRRIEAVAGDAF+EYV ARD +M+ LC+ LKVKAED
Sbjct: 783 GTHVSNTAEIRGFKIISEQGIASGVRRIEAVAGDAFVEYVCARDNYMRCLCSSLKVKAED 842

Query: 881 VTTRVENLVEDLRVL--------------------------------VEYMDDTDADSLK 940
           V  RVE ++E+LR                                  VE M D DAD LK
Sbjct: 843 VNGRVETILEELRTTRNEVSSLRSKIAVLKAASLANKATTIDNTRVVVENMGDVDADGLK 902

Query: 941 GAAEFLMDNLQDPAAIVLGSCPGEGKVSLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGG 992
            AAE+L+D L+DPAA++LGS PG+GKVSLVAAF+P VV +G+QA  F+G IAKLCGGGGG
Sbjct: 903 SAAEYLVDTLEDPAAVILGSSPGDGKVSLVAAFSPGVVKMGIQAGKFVGGIAKLCGGGGG 962

BLAST of Cp4.1LG05g13540 vs. Swiss-Prot
Match: SYAP_ORYSJ (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica GN=Os06g0245800 PE=3 SV=2)

HSP 1 Score: 1285.8 bits (3326), Expect = 0.0e+00
Identity = 643/936 (68.70%), Postives = 757/936 (80.88%), Query Frame = 1

Query: 101 QTFSSKRSSGHTTQNKKYITSVKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVL 160
           ++  S R  G   +      SV+   +E+    S +   SGD+IRRRFL FYA+RGHK+L
Sbjct: 63  RSMGSSRERGVLVKTSSSSASVESATQEVGAASSGEW--SGDAIRRRFLDFYAARGHKIL 122

Query: 161 PSASLVPEDPTVLLTIAGMLQFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHT 220
           PS+SLVP+DPTV LTIAGMLQFKPIFLGK PR VPCA T+Q+CIRTND+ENVGRT+RH T
Sbjct: 123 PSSSLVPDDPTVFLTIAGMLQFKPIFLGKEPRRVPCATTSQKCIRTNDIENVGRTSRHQT 182

Query: 221 FFEMLGNFSFGDYFKKEAIKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPV 280
           FFEMLGNFSFGDYFKKEAI WAWELTT EFGLP  RLWIS++++DDEAF+IWH+EVGVP 
Sbjct: 183 FFEMLGNFSFGDYFKKEAITWAWELTTKEFGLPPERLWISVFQDDDEAFSIWHNEVGVPK 242

Query: 281 ERIKRMGEDDNFWTSGITGPCGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYN 340
           ERIKR+GEDDNFWTSG TGPCGPCSEIYYDF PE+G SD DLGDD+RF+EFYNLVFMQYN
Sbjct: 243 ERIKRLGEDDNFWTSGATGPCGPCSEIYYDFYPERGSSDADLGDDSRFIEFYNLVFMQYN 302

Query: 341 KKDDGSLEPLKQKNIDTGLGLERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADN 400
           KKDDGSLEPLKQKNIDTG+GLERMARILQKVPNNYETDLI+PII+KA+ +A V+Y TAD+
Sbjct: 303 KKDDGSLEPLKQKNIDTGMGLERMARILQKVPNNYETDLIFPIIEKAASMALVSYTTADD 362

Query: 401 QSKTSLKIIGDHMRAIVYLISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTD 460
             KT+LKIIGDHMRA+VYLISDGV+PSNIGRGY+VRRLIRRVVRTGRL+GI+GDG GN++
Sbjct: 363 AMKTNLKIIGDHMRAVVYLISDGVIPSNIGRGYVVRRLIRRVVRTGRLIGIRGDGHGNSE 422

Query: 461 GAFTPHIAEKVIALSSHIDEDVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALA 520
           GAF P +AE  I+LS+ ID DV+ R   IL EL+REELRFVQTLERGEKLL+E+L +AL+
Sbjct: 423 GAFLPSLAEVAISLSTEIDPDVESRRKSILGELQREELRFVQTLERGEKLLDELLDEALS 482

Query: 521 SAKEDGRFPCLAGKDAFLLYDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAA 580
           SA  +G  PCL+GKD FLLYDTYGFPVEIT E++ ERGV+VDMKGFD+EM+NQR+QSQAA
Sbjct: 483 SAGNNGGKPCLSGKDVFLLYDTYGFPVEITAEISGERGVIVDMKGFDMEMENQRKQSQAA 542

Query: 581 HNAVKLEVGEGADIMENISDTEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNR 640
           HN VKL VG   +I+++I DTEFLGYD LSA A+V+ L+VNG SV  VSEG DVE+ L+R
Sbjct: 543 HNVVKLSVGNETEIVKSIPDTEFLGYDSLSATAVVKGLLVNGNSVNVVSEGSDVEIFLDR 602

Query: 641 TPFYAESGGQIGDHGFIYI-SEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGRE 700
           TPFYAESGGQ+GD+GF+Y+  E + +   V+E+ DVQKS G+IFVHKGTIKQG +EVG+E
Sbjct: 603 TPFYAESGGQVGDNGFLYVYGEEDAKQKAVIEINDVQKSLGNIFVHKGTIKQGSVEVGKE 662

Query: 701 VKAEVDAKLRQGAKVTLFAC------------QETSQAGSLVAFDRLRFDFNYHRPLADT 760
           + A VDAKLRQGAK    A              ETSQAGSLVAFDRLRFDFN+HRPL++ 
Sbjct: 663 IDAAVDAKLRQGAKAHHTATHLLQSALKSIIGSETSQAGSLVAFDRLRFDFNFHRPLSEE 722

Query: 761 EILQIEELINGWIGDAVLLQTKVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCG 820
           E+++IE L+N W+  A  L+TKVM L +AK+AGAIAMFGEKYGE+VRVVEVPGVSMELCG
Sbjct: 723 ELMKIESLVNQWVSSATHLETKVMDLQDAKNAGAIAMFGEKYGEQVRVVEVPGVSMELCG 782

Query: 821 GTHVFNTSEIRGFKVISEQGIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAED 880
           GTHV NT+EIRGFK+ISEQGIASGVRRIEAVAGDAF+EYV ARD +M+ LC+ LKVKAED
Sbjct: 783 GTHVSNTAEIRGFKIISEQGIASGVRRIEAVAGDAFVEYVCARDNYMRCLCSSLKVKAED 842

Query: 881 VTTRVENLVEDLRVL--------------------------------VEYMDDTDADSLK 940
           V  RVE ++E+LR                                  VE M D DAD LK
Sbjct: 843 VNGRVETILEELRTTRNEVSSLRSKIAVLKAASLANKATTIDNTRVVVENMGDVDADGLK 902

Query: 941 GAAEFLMDNLQDPAAIVLGSCPGEGKVSLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGG 992
            AAE+L+D L+DPAA++LGS PG+GKVSLVAAF+P VV +G+QA  F+G IAKLCGGGGG
Sbjct: 903 SAAEYLVDTLEDPAAVILGSSPGDGKVSLVAAFSPGVVKMGIQAGKFVGGIAKLCGGGGG 962

BLAST of Cp4.1LG05g13540 vs. Swiss-Prot
Match: SYAP_SORBI (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Sorghum bicolor GN=Sb10g008780 PE=3 SV=1)

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 633/917 (69.03%), Postives = 746/917 (81.35%), Query Frame = 1

Query: 121 SVKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGML 180
           SV+P  +EL    + +   SGD+IRRRFL+FYA+RGHK+LPS+SLVP+DPTVLLTIAGML
Sbjct: 47  SVEPATQELGTAGAGEW--SGDAIRRRFLEFYAARGHKILPSSSLVPDDPTVLLTIAGML 106

Query: 181 QFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK 240
           QFKPIFLGK PR VPCA T+Q+CIRTND+ENVGRTARH TFFEMLGNFSFGDYFKKEA  
Sbjct: 107 QFKPIFLGKEPRRVPCATTSQKCIRTNDIENVGRTARHQTFFEMLGNFSFGDYFKKEATA 166

Query: 241 WAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGP 300
           WAWEL T E+GLPA RLWIS++E+D+EAF IWH+EVGVP ERIKRMG +DNFWTSG TGP
Sbjct: 167 WAWELATKEYGLPAERLWISVFEDDNEAFDIWHNEVGVPKERIKRMGAEDNFWTSGATGP 226

Query: 301 CGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLG 360
           CGPCSEIYYDF PE+G SD DLGDD+RF+EFYNLVFMQYNKKDDGSLEPLKQKNIDTG+G
Sbjct: 227 CGPCSEIYYDFYPERGSSDADLGDDSRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGMG 286

Query: 361 LERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLI 420
           LERMARILQKVPNNYETDLI+PII+KA+ +A V+Y   D+ +KT+LKIIGDHMRA+VYL+
Sbjct: 287 LERMARILQKVPNNYETDLIFPIIEKAASMAMVSYAKTDDATKTNLKIIGDHMRAVVYLV 346

Query: 421 SDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDE 480
           SDGV+PSNIGRGY+VRRLIRRVVR GRL+GI+GDG GN++GAF P +AE VI+LS+ ID 
Sbjct: 347 SDGVIPSNIGRGYVVRRLIRRVVRMGRLIGIRGDGHGNSEGAFLPSLAEVVISLSTEIDP 406

Query: 481 DVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLY 540
           DV+ R   IL EL+REELRFVQTLERGEKLL+E+L  A+ SA  +G  P L+GKD FLLY
Sbjct: 407 DVESRRRSILGELQREELRFVQTLERGEKLLDELLDGAVLSAGNNGDKPSLSGKDLFLLY 466

Query: 541 DTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISD 600
           DTYGFPVEIT E+A ERGV VD++GFDIEM+NQR+QSQAAHN VKL VG   +I+++I D
Sbjct: 467 DTYGFPVEITAEIASERGVTVDIEGFDIEMENQRKQSQAAHNVVKLSVGNETEIIKSIPD 526

Query: 601 TEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYIS 660
           T FLGYD LSA A+V  L+VNG  V +VSEG +VE+LL+RTPFYAESGGQ+GD+GF+Y++
Sbjct: 527 TVFLGYDSLSASAVVRGLLVNGNPVNEVSEGSEVEILLDRTPFYAESGGQVGDNGFLYVN 586

Query: 661 EGENRGN-VVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFAC 720
            GE+R    V+E+ DVQKS G+IFVHKGTIKQG +EVG+EV A VDAKLRQGAK    A 
Sbjct: 587 GGEDRSQAAVIEINDVQKSLGNIFVHKGTIKQGSVEVGKEVDACVDAKLRQGAKAHHTAT 646

Query: 721 ------------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQ 780
                        ETSQAGSLVAFDRLRFDFN+HRP+++ E+ +IE L+N WI +A  L+
Sbjct: 647 HLLQSALKSVVGSETSQAGSLVAFDRLRFDFNFHRPVSEGELTRIELLVNQWISNAAHLE 706

Query: 781 TKVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQG 840
           TKVM L +AK+AGAIAMFGEKYGE+VRVVEVPGVS+ELCGGTHV NT+EIRGFK+ISEQG
Sbjct: 707 TKVMALQDAKNAGAIAMFGEKYGEQVRVVEVPGVSLELCGGTHVSNTAEIRGFKIISEQG 766

Query: 841 IASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRVL----- 900
           IASG+RRIEAVAGDAFI+YV ARD +M+RLC+ LKVKAEDV  RVE ++E+LR       
Sbjct: 767 IASGIRRIEAVAGDAFIDYVCARDNYMRRLCSSLKVKAEDVNGRVETILEELRATRNEVS 826

Query: 901 -----------------------------VEYMDDTDADSLKGAAEFLMDNLQDPAAIVL 960
                                        VE M D DAD LK AAE+L+  LQDPAA++L
Sbjct: 827 SLRSKIAVLKAASLASKATTVEPQNVRIVVENMGDVDADGLKSAAEYLIGTLQDPAAVIL 886

Query: 961 GSCPGEGKVSLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLN 991
           GS PG+GKVSLVAAF+P VV +G+QA  F+G IAKLCGGGGGG+PNFAQAGGRKPENL +
Sbjct: 887 GSSPGDGKVSLVAAFSPAVVKMGLQAGKFVGGIAKLCGGGGGGKPNFAQAGGRKPENLPD 946

BLAST of Cp4.1LG05g13540 vs. Swiss-Prot
Match: SYAP_POPTR (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Populus trichocarpa GN=POPTRDRAFT_821063 PE=3 SV=1)

HSP 1 Score: 1221.5 bits (3159), Expect = 0.0e+00
Identity = 597/774 (77.13%), Postives = 685/774 (88.50%), Query Frame = 1

Query: 121 SVKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGML 180
           SV+PV EELVEDK+K+  VSGD+IRRRFL+FYASR HKVLPSASLVP+DPTVLLTIAGML
Sbjct: 80  SVQPVTEELVEDKTKENPVSGDAIRRRFLEFYASRSHKVLPSASLVPDDPTVLLTIAGML 139

Query: 181 QFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK 240
           QFKPIFLGK PR VP A TAQ+CIRTNDVENVGRT RHHTFFEMLGNFSFGDYFKKEAIK
Sbjct: 140 QFKPIFLGKAPRQVPRATTAQKCIRTNDVENVGRTTRHHTFFEMLGNFSFGDYFKKEAIK 199

Query: 241 WAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGP 300
           WAWEL+T EFGLPA+RLW+S+YE+DDEAF IWHDEVGVPVERIKRMGE+DNFWTSG TGP
Sbjct: 200 WAWELSTKEFGLPADRLWVSVYEDDDEAFEIWHDEVGVPVERIKRMGEEDNFWTSGATGP 259

Query: 301 CGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLG 360
           CGPCSE+YYDF PE+G+ + DLGDD+RF+EFYNLVFMQYNK DDGSLEPLKQKNIDTGLG
Sbjct: 260 CGPCSELYYDFHPERGYKNTDLGDDSRFIEFYNLVFMQYNKMDDGSLEPLKQKNIDTGLG 319

Query: 361 LERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLI 420
           LER+ARILQKVPNNYETDLIYPII+KA+ELA+++Y  AD+++K +LKIIGDH+RAIVYLI
Sbjct: 320 LERLARILQKVPNNYETDLIYPIIEKAAELANISYALADDRTKMNLKIIGDHLRAIVYLI 379

Query: 421 SDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDE 480
           SDGV+PSNIGRGY+VRRLIRR VRTGRLLG+KG G+   DG F P IAEKVI LS HID 
Sbjct: 380 SDGVLPSNIGRGYVVRRLIRRAVRTGRLLGVKGGGE---DGVFLPAIAEKVIELSPHIDP 439

Query: 481 DVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLY 540
           DVK R   IL+EL+REELRFVQTLERGEKLL++MLA+AL +A++    PCL+GKD FLLY
Sbjct: 440 DVKARGHSILDELQREELRFVQTLERGEKLLDQMLAEALLNAQKSETLPCLSGKDVFLLY 499

Query: 541 DTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISD 600
           DT+GFPVEITTEVA+E+GV +DM GF++EM+NQRRQSQAAHN VKL V  G D+ EN+ D
Sbjct: 500 DTFGFPVEITTEVAEEQGVKIDMDGFEVEMENQRRQSQAAHNVVKLAVENGGDLAENVHD 559

Query: 601 TEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYIS 660
           TEFLGYD LSA+A+VESL++NGKSV+QVSEG +VEVLLN+TPFYAESGGQIGDHGF+Y++
Sbjct: 560 TEFLGYDTLSARAVVESLLLNGKSVIQVSEGSEVEVLLNKTPFYAESGGQIGDHGFLYVT 619

Query: 661 EGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFAC- 720
           + +++   V+E+KDVQKS GS+FVHKGTI++GVLEVGREV+A VDAKLRQ AKV   A  
Sbjct: 620 QDQSKQTAVVEIKDVQKSLGSVFVHKGTIREGVLEVGREVEAAVDAKLRQRAKVHHTATH 679

Query: 721 -----------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQT 780
                      QETSQAGSLVAFDRLRFDFN+HRPL D+E+ +IE LINGWIGD  LLQT
Sbjct: 680 LLQSALKKVIGQETSQAGSLVAFDRLRFDFNFHRPLHDSELEEIENLINGWIGDGTLLQT 739

Query: 781 KVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGI 840
           KVM L +AK AGAIAMFGEKYGE+VRVVEVPGVSMELCGGTHV NTSEIR FK+ISEQGI
Sbjct: 740 KVMSLTDAKEAGAIAMFGEKYGEQVRVVEVPGVSMELCGGTHVSNTSEIRAFKIISEQGI 799

Query: 841 ASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRVL 883
           ASG+RRIEAVAG+AFIEY+NARD  MK LC+ LKVKAE+VTTRV+NL+E+LR +
Sbjct: 800 ASGIRRIEAVAGEAFIEYINARDSQMKLLCSTLKVKAEEVTTRVDNLLEELRTV 850

BLAST of Cp4.1LG05g13540 vs. Swiss-Prot
Match: SYAP_ARATH (Alanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=EMB86 PE=1 SV=2)

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 633/924 (68.51%), Postives = 735/924 (79.55%), Query Frame = 1

Query: 116 KKYITSVKPVNEELVED-KSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLL 175
           K    SV+PV+E+  ED +SKD  VSGDSIRRRFL+F+ASRGHKVLPS+SLVPEDPTVLL
Sbjct: 56  KSSSVSVQPVSEDAKEDYQSKD--VSGDSIRRRFLEFFASRGHKVLPSSSLVPEDPTVLL 115

Query: 176 TIAGMLQFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYF 235
           TIAGMLQFKPIFLGKVPR VPCA TAQRCIRTND+ENVG+TARHHTFFEMLGNFSFGDYF
Sbjct: 116 TIAGMLQFKPIFLGKVPREVPCATTAQRCIRTNDLENVGKTARHHTFFEMLGNFSFGDYF 175

Query: 236 KKEAIKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWT 295
           KKEAIKWAWEL+TIEFGLPANR+W+SIYE+DDEAF IW +EVGV VERIKRMGE DNFWT
Sbjct: 176 KKEAIKWAWELSTIEFGLPANRVWVSIYEDDDEAFEIWKNEVGVSVERIKRMGEADNFWT 235

Query: 296 SGITGPCGPCSEIYYDFQPEKGHS-DVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQK 355
           SG TGPCGPCSE+YYDF PE+G+  DVDLGDDTRF+EFYNLVFMQYNK +DG LEPLKQK
Sbjct: 236 SGPTGPCGPCSELYYDFYPERGYDEDVDLGDDTRFIEFYNLVFMQYNKTEDGLLEPLKQK 295

Query: 356 NIDTGLGLERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHM 415
           NIDTGLGLER+A+ILQKVPNNYETDLIYPII K SELA+++YD+A++++KTSLK+I DHM
Sbjct: 296 NIDTGLGLERIAQILQKVPNNYETDLIYPIIAKISELANISYDSANDKAKTSLKVIADHM 355

Query: 416 RAIVYLISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIA 475
           RA+VYLISDGV PSNIGRGY+VRRLIRR VR G+ LGI GD  GN  GAF P +AEKVI 
Sbjct: 356 RAVVYLISDGVSPSNIGRGYVVRRLIRRAVRKGKSLGINGDMNGNLKGAFLPAVAEKVIE 415

Query: 476 LSSHIDEDVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAG 535
           LS++ID DVK +  RI+EE+++EEL F +TLERGEKLL++ L DAL+ A +    P L G
Sbjct: 416 LSTYIDSDVKLKASRIIEEIRQEELHFKKTLERGEKLLDQKLNDALSIADKTKDTPYLDG 475

Query: 536 KDAFLLYDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGAD 595
           KDAFLLYDT+GFPVEIT EVA+ERGV +DM GF++EM+NQRRQSQAAHN VKL V + AD
Sbjct: 476 KDAFLLYDTFGFPVEITAEVAEERGVSIDMNGFEVEMENQRRQSQAAHNVVKLTVEDDAD 535

Query: 596 IMENISDTEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGD 655
           + +NI+DTEFLGYD LSA+A+V+SL+VNGK V++VSEG +VEVLL+RTPFYAESGGQI D
Sbjct: 536 MTKNIADTEFLGYDSLSARAVVKSLLVNGKPVIRVSEGSEVEVLLDRTPFYAESGGQIAD 595

Query: 656 HGFIYISEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAK 715
           HGF+Y+S   N+   V+EV DVQKS   IFVHKGT+K G LEVG+EV+A VDA LRQ AK
Sbjct: 596 HGFLYVSSDGNQEKAVVEVSDVQKSL-KIFVHKGTVKSGALEVGKEVEAAVDADLRQRAK 655

Query: 716 VTLFAC------------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIG 775
           V   A             QETSQAGSLVAFDRLRFDFN++R L D E+ +IE LIN WIG
Sbjct: 656 VHHTATHLLQSALKKVVGQETSQAGSLVAFDRLRFDFNFNRSLHDNELEEIECLINRWIG 715

Query: 776 DAVLLQTKVMPLAEAKSAGAIAMFGEKYGE-EVRVVEVPGVSMELCGGTHVFNTSEIRGF 835
           DA  L+TKV+PLA+AK AGAIAMFGEKY E EVRVVEVPGVSMELCGGTHV NT+EIR F
Sbjct: 716 DATRLETKVLPLADAKRAGAIAMFGEKYDENEVRVVEVPGVSMELCGGTHVGNTAEIRAF 775

Query: 836 KVISEQGIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLR 895
           K+ISEQGIASG+RRIEAVAG+AFIEY+N+RD  M RLC+ LKVKAEDVT RVENL+E+LR
Sbjct: 776 KIISEQGIASGIRRIEAVAGEAFIEYINSRDSQMTRLCSTLKVKAEDVTNRVENLLEELR 835

Query: 896 VLVEYMDDTDADSLKGAA----------------EFLMDNLQDPAAIVLGSCPG------ 955
              +   D  + +    A                  L++++ D  A  L S         
Sbjct: 836 AARKEASDLRSKAAVYKASVISNKAFTVGTSQTIRVLVESMDDTDADSLKSAAEHLISTL 895

Query: 956 EGKVSLVAAFTPTVVDLGV-------------QARNFIGPIAKLCGGGGGGRPNFAQAGG 990
           E  V++V   +P    + +             QA  FIGPIAKLCGGGGGG+PNFAQAGG
Sbjct: 896 EDPVAVVLGSSPEKDKVSLVAAFSPGVVSLGVQAGKFIGPIAKLCGGGGGGKPNFAQAGG 955

BLAST of Cp4.1LG05g13540 vs. TrEMBL
Match: A0A059ANQ5_EUCGR (Probable alanine--tRNA ligase, chloroplastic OS=Eucalyptus grandis GN=EUGRSUZ_I01492 PE=3 SV=1)

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 694/918 (75.60%), Postives = 780/918 (84.97%), Query Frame = 1

Query: 121 SVKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGML 180
           SV+PV EEL+E+KS DL VSGDSIRRRFL FYASRGHKVLPSASLVP+DPTVLLTIAGML
Sbjct: 68  SVQPVTEELIEEKSNDLLVSGDSIRRRFLDFYASRGHKVLPSASLVPDDPTVLLTIAGML 127

Query: 181 QFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK 240
           QFKPIFLGKVPR VPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK
Sbjct: 128 QFKPIFLGKVPRQVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK 187

Query: 241 WAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGP 300
           WAWEL+T E GL A RLWIS++E+DDEAF IWH EVGVP + IKRMGE+DNFWTSG+TGP
Sbjct: 188 WAWELSTAELGLQAERLWISVFEDDDEAFEIWHKEVGVPADHIKRMGEEDNFWTSGVTGP 247

Query: 301 CGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLG 360
           CGPCSEIYYDF PE+G  D DLGDD+RF+EFYNLVFMQYNKKDDGSLEPLKQKNIDTG+G
Sbjct: 248 CGPCSEIYYDFHPERGTLDTDLGDDSRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGMG 307

Query: 361 LERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLI 420
           LER+ARILQ+VP+NYETDLIYPII+KASELA+V+Y  AD+ +K  LKIIGDHMRAIVYLI
Sbjct: 308 LERVARILQRVPSNYETDLIYPIIEKASELANVSYGLADDPTKLKLKIIGDHMRAIVYLI 367

Query: 421 SDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDE 480
           SDGV+PSNIGRGY+VRRLIRRVVRTGRLLGI+GD + N +GAF P IAEKVI   +HID 
Sbjct: 368 SDGVIPSNIGRGYVVRRLIRRVVRTGRLLGIRGDCRENLEGAFLPAIAEKVIDFGTHIDS 427

Query: 481 DVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLY 540
           DVK R  RILEE+KREE RFVQTLERGE+LLE+M+ DAL+S +E G  PCLAG+DAFLLY
Sbjct: 428 DVKARAPRILEEIKREEYRFVQTLERGEELLEQMIDDALSSTREKGTVPCLAGEDAFLLY 487

Query: 541 DTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISD 600
           DTYGFPVEIT EVA+E GV VDM  FD EM+ QRR+SQAAH+A+KLEVG+ ADI  NI D
Sbjct: 488 DTYGFPVEITIEVAEEHGVTVDMNAFDTEMEKQRRRSQAAHHAIKLEVGDDADITANIPD 547

Query: 601 TEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYIS 660
           TEFLGYD LSA+A+++SL+VNGKSV+QV EG +VEVLL++TPFYAESGGQI DHG + +S
Sbjct: 548 TEFLGYDTLSARAVIKSLLVNGKSVIQVPEGSEVEVLLDKTPFYAESGGQIADHGLLCVS 607

Query: 661 EGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFAC- 720
           +GEN+   V+E+ DVQKS G++FVHKGTIK+GVLEVG EV+AEVD KLRQ AKV   A  
Sbjct: 608 DGENQQRTVVEIMDVQKSMGNLFVHKGTIKEGVLEVGIEVEAEVDPKLRQRAKVHHTATH 667

Query: 721 -----------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQT 780
                      QETSQAGSLVAFDRLRFDFN+HRPL + EIL+IE LINGWIGDA +LQT
Sbjct: 668 LLQSALKKVIGQETSQAGSLVAFDRLRFDFNFHRPLLEDEILEIERLINGWIGDAAVLQT 727

Query: 781 KVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGI 840
           KVMPL EAK AGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHV NT+EIRGFK+ISEQGI
Sbjct: 728 KVMPLTEAKEAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVSNTAEIRGFKIISEQGI 787

Query: 841 ASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENL------------- 900
           ASG+RRIEAVAG+AFIEYVN+RDY+MK+LC  LKVKAE+VTTRV+NL             
Sbjct: 788 ASGIRRIEAVAGEAFIEYVNSRDYYMKQLCNNLKVKAEEVTTRVDNLLEELRMARNEVSL 847

Query: 901 ----------------------VEDLRVLVEYMDDTDADSLKGAAEFLMDNLQDPAAIVL 960
                                  +++RVLVE M+DTDADSLK AAE+L+D+LQDPAA+VL
Sbjct: 848 LRSKAAVYKASTIASKAVLVGSSKEIRVLVESMEDTDADSLKSAAEYLIDSLQDPAAVVL 907

Query: 961 GSCPGEGKVSLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLN 992
           GS P EGKVSLVAAFTP VV LG+QA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENL +
Sbjct: 908 GSSPSEGKVSLVAAFTPGVVKLGIQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLSS 967

BLAST of Cp4.1LG05g13540 vs. TrEMBL
Match: A0A0A0KE77_CUCSA (Alanine--tRNA ligase OS=Cucumis sativus GN=Csa_6G127290 PE=3 SV=1)

HSP 1 Score: 1357.8 bits (3513), Expect = 0.0e+00
Identity = 703/851 (82.61%), Postives = 741/851 (87.07%), Query Frame = 1

Query: 188 GKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTT 247
           G VPRLVPCAAT+QRC+RTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTT
Sbjct: 45  GTVPRLVPCAATSQRCLRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTT 104

Query: 248 IEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCSEI 307
           +EFGLPANRLWISIYEEDDEAFAIWHDEVGVP++RIKRMGEDDNFWTSGITGPCGPCSEI
Sbjct: 105 VEFGLPANRLWISIYEEDDEAFAIWHDEVGVPIDRIKRMGEDDNFWTSGITGPCGPCSEI 164

Query: 308 YYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARI 367
           YYDFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARI
Sbjct: 165 YYDFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARI 224

Query: 368 LQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLISDGVVPS 427
           LQKVPNNYETDLIYPIIQKASELA+VTYDTADN+SKT+LKIIGDHMRA+VYLISDGVVPS
Sbjct: 225 LQKVPNNYETDLIYPIIQKASELANVTYDTADNRSKTNLKIIGDHMRAVVYLISDGVVPS 284

Query: 428 NIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVKGRTG 487
           NIGRGYIVRRLIRRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALS+HIDEDVKGR G
Sbjct: 285 NIGRGYIVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPVIAEKVIALSNHIDEDVKGRAG 344

Query: 488 RILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLYDTYGFPV 547
           RILEELKREELRFVQTLERGEKLLEEMLADALASA + GR PCLAGKDAFLLYDTYGFPV
Sbjct: 345 RILEELKREELRFVQTLERGEKLLEEMLADALASANDGGRIPCLAGKDAFLLYDTYGFPV 404

Query: 548 EITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEFLGYD 607
           EI+TEVAQERGVVVDM+GFDIEMDNQRRQSQAAHN VKLEVGEGADIMENISDTEFLGYD
Sbjct: 405 EISTEVAQERGVVVDMEGFDIEMDNQRRQSQAAHNVVKLEVGEGADIMENISDTEFLGYD 464

Query: 608 MLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGENRGN 667
            LSAKAIVESLIVNG  VLQVSEG+DVEVLLNRTPFYAESGGQIGDHGFIYISEGEN  N
Sbjct: 465 TLSAKAIVESLIVNGNPVLQVSEGNDVEVLLNRTPFYAESGGQIGDHGFIYISEGENSQN 524

Query: 668 VVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFAC-------- 727
           VV+EVKDVQKSAGSIFVHKG IKQG+LEVGREV+A VDA+LRQGAKV   A         
Sbjct: 525 VVVEVKDVQKSAGSIFVHKGIIKQGILEVGREVEAAVDAELRQGAKVHHTATHLLQSALK 584

Query: 728 ----QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVMPLAE 787
               QETSQAGSLVAFDRLRFDFNYHRPL D EI++IEELINGWIGDAVLLQTKVM L E
Sbjct: 585 RIIGQETSQAGSLVAFDRLRFDFNYHRPLVDAEIVKIEELINGWIGDAVLLQTKVMALTE 644

Query: 788 AKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGIASGVRRI 847
           AK AGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQGIASGVRRI
Sbjct: 645 AKKAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGIASGVRRI 704

Query: 848 EAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRV-------LVEYMDD 907
           EAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRV+NL+E+LR+       L E    
Sbjct: 705 EAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVDNLLEELRMARNEISNLREKTAV 764

Query: 908 TDADSLKGAA---------EFLMDNLQDPAAIVLGSCPG------EGKVSLVAAFTPTVV 967
             A S+   A           L++ + D  A  L S         +  V++V    P   
Sbjct: 765 AKASSIANKAFVVGTSKEIRVLVEYMDDADADSLKSAAEFLMDNLQDPVAIVLGSCPGEG 824

Query: 968 DLGVQAR-------------NFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALENART 992
            + + A               FIG IAKLCGGGGGGRPNFAQAGGRKPENLL+ALENAR+
Sbjct: 825 KVSLVAAFTPSVVDLGVQAGKFIGSIAKLCGGGGGGRPNFAQAGGRKPENLLDALENARS 884

BLAST of Cp4.1LG05g13540 vs. TrEMBL
Match: D7U4W0_VITVI (Probable alanine--tRNA ligase, chloroplastic OS=Vitis vinifera GN=VIT_03s0038g03440 PE=3 SV=1)

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 695/943 (73.70%), Postives = 778/943 (82.50%), Query Frame = 1

Query: 96   HHGLSQTFSSKRSSGHTTQNKKYITSVKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASR 155
            H  L QT    R    TT++     S++P+ EELVEDKSKDL  SGDSIR RFL FYASR
Sbjct: 70   HSNLKQT----REVRFTTRSTS--ASIQPMTEELVEDKSKDLPTSGDSIRHRFLDFYASR 129

Query: 156  GHKVLPSASLVPEDPTVLLTIAGMLQFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRT 215
            GHKVLPS+SLVP+DPTVLLTIAGMLQFKPIFLGKVPR VP A TAQRCIRTNDVENVG+T
Sbjct: 130  GHKVLPSSSLVPDDPTVLLTIAGMLQFKPIFLGKVPRQVPRATTAQRCIRTNDVENVGKT 189

Query: 216  ARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDE 275
            +RHHTFFEMLGNFSFGDYFKKEAIKWAWEL+TIE+GLPA+RLWIS+YE+DDEA AIW  E
Sbjct: 190  SRHHTFFEMLGNFSFGDYFKKEAIKWAWELSTIEYGLPADRLWISVYEDDDEALAIWTKE 249

Query: 276  VGVPVERIKRMGEDDNFWTSGITGPCGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLV 335
            VGVPVERIKRMG +DNFWTSG+TGPCGPCSEIYYDF PE+G+SDVDLGDDTRF+EFYNLV
Sbjct: 250  VGVPVERIKRMGAEDNFWTSGVTGPCGPCSEIYYDFHPERGYSDVDLGDDTRFIEFYNLV 309

Query: 336  FMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQKVPNNYETDLIYPIIQKASELAHVTY 395
            FMQYNKKDDGSLEPLKQ NIDTGLGLERMARILQKVPNNYETDLIYPII+KASELA+V+Y
Sbjct: 310  FMQYNKKDDGSLEPLKQMNIDTGLGLERMARILQKVPNNYETDLIYPIIEKASELANVSY 369

Query: 396  DTADNQSKTSLKIIGDHMRAIVYLISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDG 455
              AD+ +K +LK+IGDH+RAIVYLISDGVVPSNIGRGY+ RRLIRR VRTGRLLGIKGDG
Sbjct: 370  ALADDHAKMNLKVIGDHLRAIVYLISDGVVPSNIGRGYVARRLIRRAVRTGRLLGIKGDG 429

Query: 456  KGNTDGAFTPHIAEKVIALSSHIDEDVKGRTGRILEELKREELRFVQTLERGEKLLEEML 515
            +GN +GAF P IAEKVI LSS ID DVK R  RILEELKREELRFVQTLERGEKLL+EML
Sbjct: 430  RGNPEGAFLPTIAEKVIELSSQIDPDVKSRAPRILEELKREELRFVQTLERGEKLLDEML 489

Query: 516  ADALASAKEDGRFPCLAGKDAFLLYDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRR 575
            A+AL ++ E+G  P L+GKD FLLYDTYGFPVEITTE A+ERGV +DM GF+IEM+NQRR
Sbjct: 490  ANALLNSNENGNGPILSGKDVFLLYDTYGFPVEITTEAAEERGVGIDMNGFEIEMENQRR 549

Query: 576  QSQAAHNAVKLEVGEGADIMENISDTEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVE 635
            QSQAAHNAVKL VG  AD+ ENISDTEFLGY+ LS KA++E L+VNG  V+QVSEG DVE
Sbjct: 550  QSQAAHNAVKLAVGNSADLTENISDTEFLGYETLSTKAVIEGLLVNGNPVIQVSEGSDVE 609

Query: 636  VLLNRTPFYAESGGQIGDHGFIYISEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLE 695
            + LNRTPFYAESGGQIGDHGF+Y++E  N+ N V+E+KDVQKS G+IFVHKGTIK+GV+E
Sbjct: 610  IFLNRTPFYAESGGQIGDHGFLYVNEDRNQKNAVVEIKDVQKSLGNIFVHKGTIKEGVVE 669

Query: 696  VGREVKAEVDAKLRQGAKVTLFAC------------QETSQAGSLVAFDRLRFDFNYHRP 755
            VG+EV+A VDA LRQ AK+   A              ETSQAGSLVAFDRLRFDFN+HRP
Sbjct: 670  VGKEVEAAVDANLRQRAKIHHTATHLLQAALKKVIGDETSQAGSLVAFDRLRFDFNFHRP 729

Query: 756  LADTEILQIEELINGWIGDAVLLQTKVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSM 815
            L + E+++IEELINGWIGDA LLQTKVMPLA+AK AGAIAMFGEKYGE+VRVVEVPGVSM
Sbjct: 730  LQEKELVEIEELINGWIGDATLLQTKVMPLADAKRAGAIAMFGEKYGEQVRVVEVPGVSM 789

Query: 816  ELCGGTHVFNTSEIRGFKVISEQGIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKV 875
            ELCGGTHV NT EIRGFK+ISEQGIASG+RRIEAVAGDAFIEYVNARD HM++LC+ LKV
Sbjct: 790  ELCGGTHVSNTCEIRGFKIISEQGIASGIRRIEAVAGDAFIEYVNARDNHMRQLCSTLKV 849

Query: 876  KAEDVTTRVENLVEDLRVLVEYMDDTDADS-------LKGAA---------EFLMDNLQD 935
            KAE+VTTRVE L+E+LR+    +    A +       + G A           L++++ D
Sbjct: 850  KAEEVTTRVEALLEELRMTRNEVSAVRAKAAVYKASVMAGNAFPVGTSKKIRVLVESMDD 909

Query: 936  PAAIVLGSCPG-------------------EGKVSLVAAFTPTVVDLGVQARNFIGPIAK 992
              A  L S                      E KVSLVAAFTP VVDLG+QA  FIGPIAK
Sbjct: 910  IDADSLKSAAEYLIDTLQDPAAVILGSCPSEEKVSLVAAFTPGVVDLGIQAGKFIGPIAK 969

BLAST of Cp4.1LG05g13540 vs. TrEMBL
Match: A0A161ZQE2_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_022835 PE=4 SV=1)

HSP 1 Score: 1340.9 bits (3469), Expect = 0.0e+00
Identity = 671/927 (72.38%), Postives = 782/927 (84.36%), Query Frame = 1

Query: 111 HTTQNKKYITS--VKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPE 170
           H  Q+K    S  ++P   E+V + S+D+  SGDSIRRRFL+FYASRGH+VLPS+SLVP+
Sbjct: 4   HEKQSKPRTVSATIQPTTGEVVAE-SQDVSTSGDSIRRRFLEFYASRGHRVLPSSSLVPD 63

Query: 171 DPTVLLTIAGMLQFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNF 230
           DPTVLLTIAGMLQFKPIFLG+VPR VP AAT+Q+CIRTND+ENVGRT+RH TFFEMLGNF
Sbjct: 64  DPTVLLTIAGMLQFKPIFLGQVPREVPRAATSQKCIRTNDIENVGRTSRHQTFFEMLGNF 123

Query: 231 SFGDYFKKEAIKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGE 290
           SFGDYFKKEAI+WAWEL+T+EFGLP+ RLWIS++EEDDEAF IWHDE+GVPV RIKRMGE
Sbjct: 124 SFGDYFKKEAIRWAWELSTLEFGLPSERLWISVFEEDDEAFTIWHDEIGVPVHRIKRMGE 183

Query: 291 DDNFWTSGITGPCGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLE 350
           +DNFW+SGITGPCGPCSEIYYDF PEKG++D DL DDTRF+EFYNLVFMQYNKK+DGSLE
Sbjct: 184 EDNFWSSGITGPCGPCSEIYYDFHPEKGYTDTDLNDDTRFIEFYNLVFMQYNKKEDGSLE 243

Query: 351 PLKQKNIDTGLGLERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKI 410
           PLKQ+NIDTGLGLERMARILQKVPNNYETDLI+PII+KASE+A+V Y  AD+ +KT LKI
Sbjct: 244 PLKQRNIDTGLGLERMARILQKVPNNYETDLIFPIIEKASEMANVRYALADDPTKTKLKI 303

Query: 411 IGDHMRAIVYLISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIA 470
           IGDH+RAIVYLISDGV+PSNIGRGYIVRRLIRRVVRTGRLLGIKGDG GN DGA  P +A
Sbjct: 304 IGDHLRAIVYLISDGVIPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGAGNLDGAMLPVLA 363

Query: 471 EKVIALSSHIDEDVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRF 530
           E VI LS  ID DVK R+ RILEELKREELRF+ TLERGEKLLE+MLA+A ASA+E    
Sbjct: 364 EIVIDLSPQIDIDVKSRSPRILEELKREELRFILTLERGEKLLEQMLANASASAQETSTV 423

Query: 531 PCLAGKDAFLLYDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEV 590
           P L+GKDAF+LYDTYGFPVEIT EVA ERGV +DM+ FD EM+NQRRQSQAAH+ VKL V
Sbjct: 424 PYLSGKDAFILYDTYGFPVEITMEVAAERGVSIDMEAFDSEMENQRRQSQAAHSTVKLTV 483

Query: 591 GEGADIMENISDTEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESG 650
             GAD  +NI DTEFLGY+ LSAKA+VE L++NG SVLQVSEG++V++LLNRTPFYAESG
Sbjct: 484 ENGADQTKNIPDTEFLGYEYLSAKAVVEGLLINGSSVLQVSEGNEVDILLNRTPFYAESG 543

Query: 651 GQIGDHGFIYISEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKL 710
           GQIGD GF+Y+++ EN    V+E+KDVQ+S G+IFVHKG+I++G++EVG+EV+A VD  +
Sbjct: 544 GQIGDQGFLYVTDAENHQKAVVEIKDVQRSLGNIFVHKGSIREGIIEVGKEVEAAVDKNM 603

Query: 711 RQGAKVTLFAC------------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELI 770
           RQ +KV   A             QETSQAGSLVAFDRLRFDFN++RPL + E+++IE LI
Sbjct: 604 RQRSKVHHTATHLLQAALKKVIGQETSQAGSLVAFDRLRFDFNFNRPLLENEVMEIENLI 663

Query: 771 NGWIGDAVLLQTKVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSE 830
           NGWIGDA LLQTKVMP+ +AK AGAIAMFGEKYG++VRVVEVPGVSMELCGGTHV NT+E
Sbjct: 664 NGWIGDASLLQTKVMPIVDAKRAGAIAMFGEKYGDQVRVVEVPGVSMELCGGTHVSNTAE 723

Query: 831 IRGFKVISEQGIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVE--- 890
           IRGFK+ISEQGIASG+RRIEAVAG+AFIEYV+ARD +MK+LC+ LKVKAE+VTTRV+   
Sbjct: 724 IRGFKIISEQGIASGIRRIEAVAGEAFIEYVSARDNYMKQLCSTLKVKAEEVTTRVDALL 783

Query: 891 -------NLVED------------------------LRVLVEYMDDTDADSLKGAAEFLM 950
                  N V +                        +RVLVE MDD DADSLK AAE+++
Sbjct: 784 EELRNTKNEVSEVRSKAAVDKASVIASKAFTVGNSKIRVLVECMDDVDADSLKSAAEYIV 843

Query: 951 DNLQDPAAIVLGSCPGEGKVSLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQ 990
           D LQDPAA++LGSCPGEGKVSLVAAFTP VV+LG+QA  FIGPIAK+CGGGGGGRPNFAQ
Sbjct: 844 DTLQDPAAVILGSCPGEGKVSLVAAFTPGVVNLGLQAGKFIGPIAKICGGGGGGRPNFAQ 903

BLAST of Cp4.1LG05g13540 vs. TrEMBL
Match: W9SBC5_9ROSA (Probable alanine--tRNA ligase, chloroplastic OS=Morus notabilis GN=L484_006689 PE=3 SV=1)

HSP 1 Score: 1332.4 bits (3447), Expect = 0.0e+00
Identity = 700/1035 (67.63%), Postives = 806/1035 (77.87%), Query Frame = 1

Query: 4    TLKLPNHPPRSYGRNSLTPLQRSSLYGNKPASLVRPRRKVTCSSGYKLISIILSVEDCCE 63
            +LKLP    RS G N+L PL  S L   KP S     R      G+              
Sbjct: 3    SLKLPQSISRSNGGNALFPLPTSPLLP-KPTSPPHQHRSFALCKGF-------------- 62

Query: 64   RSPTLAISVKGVTIKCYTFFKLYYKEFCPFSVHHGLSQTFSSKRSSGHTTQNKKYITSVK 123
                    + G T+K +T          PF      SQ    K   G     +    SV+
Sbjct: 63   --------ITGKTMKLFT------PNILPFG-----SQVL--KGEWGLQFSRRSTSASVQ 122

Query: 124  PVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGMLQFK 183
            PV E+L  D+S D  VSGDSIRRRFL FYASRGHKVLPSASLVP+DPTVLLTIAGMLQFK
Sbjct: 123  PVTEQLKVDESNDPLVSGDSIRRRFLDFYASRGHKVLPSASLVPDDPTVLLTIAGMLQFK 182

Query: 184  PIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAW 243
            PIFLGKVPR VP A TAQRCIRTNDV+NVGRT RH TFFEMLGNFSFGDYFKKEAI WAW
Sbjct: 183  PIFLGKVPRQVPRATTAQRCIRTNDVDNVGRTTRHQTFFEMLGNFSFGDYFKKEAITWAW 242

Query: 244  ELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGP 303
            EL+T+E+GLPA+RLW+S+YE+DDEAFAIWHDEVGVPVERIKRMGEDDNFWTSG+TGPCGP
Sbjct: 243  ELSTVEYGLPADRLWVSVYEDDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGVTGPCGP 302

Query: 304  CSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLER 363
            CSE+YYDF PE+G+++VDLGDD+RF+EFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLER
Sbjct: 303  CSELYYDFHPERGYANVDLGDDSRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLER 362

Query: 364  MARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLISDG 423
            MARILQKVPNNYETDLIYPII++A++LA+V+Y+ AD++S+ +LKI+GDH+RAIVYLISDG
Sbjct: 363  MARILQKVPNNYETDLIYPIIERAAKLANVSYELADDRSRMNLKILGDHLRAIVYLISDG 422

Query: 424  VVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDEDVK 483
            V PSNIGRGY+VRRLIRR VRTGRLLGI GDG+GN +GAF P IAE+VI +S+H+D DVK
Sbjct: 423  VAPSNIGRGYVVRRLIRRAVRTGRLLGIYGDGRGNLEGAFLPEIAEEVIEMSTHVDVDVK 482

Query: 484  GRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLYDTY 543
            GR  RILEELKREELRFVQTLERGEKLL++MLADAL SA+E+   PCL+GKDAFLLYDTY
Sbjct: 483  GRAPRILEELKREELRFVQTLERGEKLLDQMLADALVSAQENKTAPCLSGKDAFLLYDTY 542

Query: 544  GFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISDTEF 603
            GFPVEITTEVA+E GV +DM GFD+EM+ QRRQSQAAHNAVKL+V   AD+ E + DTEF
Sbjct: 543  GFPVEITTEVAEENGVSIDMAGFDVEMEKQRRQSQAAHNAVKLQVENSADVTERVPDTEF 602

Query: 604  LGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYISEGE 663
            LGY+ LSA AIVESL+VNGKSV+QV+EG DVEVLLNRTPFYAESGGQIGD+G++Y++EGE
Sbjct: 603  LGYETLSALAIVESLLVNGKSVIQVAEGSDVEVLLNRTPFYAESGGQIGDNGYLYVTEGE 662

Query: 664  NRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFAC---- 723
            ++   V+E+KDVQKS GSIFVHKG IK+GVLEVG+EV+A VDA LRQ AKV   A     
Sbjct: 663  SQQTAVIEIKDVQKSPGSIFVHKGVIKEGVLEVGKEVEASVDANLRQRAKVHHTATHLLQ 722

Query: 724  --------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQTKVM 783
                    QETSQAGSLVAFDRLRFDFN+HRPL D E+++IE LINGWIGDA LLQTKVM
Sbjct: 723  SALKRVIGQETSQAGSLVAFDRLRFDFNFHRPLLDRELMEIERLINGWIGDATLLQTKVM 782

Query: 784  PLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGIASG 843
            PLA+AK AGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHV NT+EIRGFK+ISEQGIASG
Sbjct: 783  PLADAKRAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVSNTAEIRGFKIISEQGIASG 842

Query: 844  VRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRVL---VEYMDD 903
            VRRIEAVAG+AFIEYVN+RDYHMK+LC+ LKVKAE+VTTRVENL+++LR     V  + +
Sbjct: 843  VRRIEAVAGEAFIEYVNSRDYHMKQLCSTLKVKAEEVTTRVENLLDELRTARNEVSALQE 902

Query: 904  TDA---DSLKGAAEF----------LMDNLQDPAAIVLGSCPG------EGKVSLVAAFT 963
              A    S+  +  F          L+++++D  A  L S         E   ++V    
Sbjct: 903  KAAVYKASILASKVFSVGTSKEIRVLVESMEDTDADALKSAAEYLISTLEDPAAVVLGSC 962

Query: 964  PTVVDLGVQAR-------------NFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLNALE 992
            P    + + A               FIGP+AKLCGGGGGGRPNFAQAGGRKPENL NALE
Sbjct: 963  PGEGKVSLVAAFTPGVVELGIQAGKFIGPVAKLCGGGGGGRPNFAQAGGRKPENLSNALE 1001

BLAST of Cp4.1LG05g13540 vs. TAIR10
Match: AT5G22800.1 (AT5G22800.1 Alanyl-tRNA synthetase, class IIc)

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 633/924 (68.51%), Postives = 735/924 (79.55%), Query Frame = 1

Query: 116 KKYITSVKPVNEELVED-KSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLL 175
           K    SV+PV+E+  ED +SKD  VSGDSIRRRFL+F+ASRGHKVLPS+SLVPEDPTVLL
Sbjct: 56  KSSSVSVQPVSEDAKEDYQSKD--VSGDSIRRRFLEFFASRGHKVLPSSSLVPEDPTVLL 115

Query: 176 TIAGMLQFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYF 235
           TIAGMLQFKPIFLGKVPR VPCA TAQRCIRTND+ENVG+TARHHTFFEMLGNFSFGDYF
Sbjct: 116 TIAGMLQFKPIFLGKVPREVPCATTAQRCIRTNDLENVGKTARHHTFFEMLGNFSFGDYF 175

Query: 236 KKEAIKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWT 295
           KKEAIKWAWEL+TIEFGLPANR+W+SIYE+DDEAF IW +EVGV VERIKRMGE DNFWT
Sbjct: 176 KKEAIKWAWELSTIEFGLPANRVWVSIYEDDDEAFEIWKNEVGVSVERIKRMGEADNFWT 235

Query: 296 SGITGPCGPCSEIYYDFQPEKGHS-DVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQK 355
           SG TGPCGPCSE+YYDF PE+G+  DVDLGDDTRF+EFYNLVFMQYNK +DG LEPLKQK
Sbjct: 236 SGPTGPCGPCSELYYDFYPERGYDEDVDLGDDTRFIEFYNLVFMQYNKTEDGLLEPLKQK 295

Query: 356 NIDTGLGLERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHM 415
           NIDTGLGLER+A+ILQKVPNNYETDLIYPII K SELA+++YD+A++++KTSLK+I DHM
Sbjct: 296 NIDTGLGLERIAQILQKVPNNYETDLIYPIIAKISELANISYDSANDKAKTSLKVIADHM 355

Query: 416 RAIVYLISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIA 475
           RA+VYLISDGV PSNIGRGY+VRRLIRR VR G+ LGI GD  GN  GAF P +AEKVI 
Sbjct: 356 RAVVYLISDGVSPSNIGRGYVVRRLIRRAVRKGKSLGINGDMNGNLKGAFLPAVAEKVIE 415

Query: 476 LSSHIDEDVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAG 535
           LS++ID DVK +  RI+EE+++EEL F +TLERGEKLL++ L DAL+ A +    P L G
Sbjct: 416 LSTYIDSDVKLKASRIIEEIRQEELHFKKTLERGEKLLDQKLNDALSIADKTKDTPYLDG 475

Query: 536 KDAFLLYDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGAD 595
           KDAFLLYDT+GFPVEIT EVA+ERGV +DM GF++EM+NQRRQSQAAHN VKL V + AD
Sbjct: 476 KDAFLLYDTFGFPVEITAEVAEERGVSIDMNGFEVEMENQRRQSQAAHNVVKLTVEDDAD 535

Query: 596 IMENISDTEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGD 655
           + +NI+DTEFLGYD LSA+A+V+SL+VNGK V++VSEG +VEVLL+RTPFYAESGGQI D
Sbjct: 536 MTKNIADTEFLGYDSLSARAVVKSLLVNGKPVIRVSEGSEVEVLLDRTPFYAESGGQIAD 595

Query: 656 HGFIYISEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAK 715
           HGF+Y+S   N+   V+EV DVQKS   IFVHKGT+K G LEVG+EV+A VDA LRQ AK
Sbjct: 596 HGFLYVSSDGNQEKAVVEVSDVQKSL-KIFVHKGTVKSGALEVGKEVEAAVDADLRQRAK 655

Query: 716 VTLFAC------------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIG 775
           V   A             QETSQAGSLVAFDRLRFDFN++R L D E+ +IE LIN WIG
Sbjct: 656 VHHTATHLLQSALKKVVGQETSQAGSLVAFDRLRFDFNFNRSLHDNELEEIECLINRWIG 715

Query: 776 DAVLLQTKVMPLAEAKSAGAIAMFGEKYGE-EVRVVEVPGVSMELCGGTHVFNTSEIRGF 835
           DA  L+TKV+PLA+AK AGAIAMFGEKY E EVRVVEVPGVSMELCGGTHV NT+EIR F
Sbjct: 716 DATRLETKVLPLADAKRAGAIAMFGEKYDENEVRVVEVPGVSMELCGGTHVGNTAEIRAF 775

Query: 836 KVISEQGIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLR 895
           K+ISEQGIASG+RRIEAVAG+AFIEY+N+RD  M RLC+ LKVKAEDVT RVENL+E+LR
Sbjct: 776 KIISEQGIASGIRRIEAVAGEAFIEYINSRDSQMTRLCSTLKVKAEDVTNRVENLLEELR 835

Query: 896 VLVEYMDDTDADSLKGAA----------------EFLMDNLQDPAAIVLGSCPG------ 955
              +   D  + +    A                  L++++ D  A  L S         
Sbjct: 836 AARKEASDLRSKAAVYKASVISNKAFTVGTSQTIRVLVESMDDTDADSLKSAAEHLISTL 895

Query: 956 EGKVSLVAAFTPTVVDLGV-------------QARNFIGPIAKLCGGGGGGRPNFAQAGG 990
           E  V++V   +P    + +             QA  FIGPIAKLCGGGGGG+PNFAQAGG
Sbjct: 896 EDPVAVVLGSSPEKDKVSLVAAFSPGVVSLGVQAGKFIGPIAKLCGGGGGGKPNFAQAGG 955

BLAST of Cp4.1LG05g13540 vs. TAIR10
Match: AT1G50200.1 (AT1G50200.1 Alanyl-tRNA synthetase)

HSP 1 Score: 428.7 bits (1101), Expect = 9.7e-120
Identity = 272/769 (35.37%), Postives = 422/769 (54.88%), Query Frame = 1

Query: 144 IRRRFLQFYASRGHKVLPSASLVPE-DPTVLLTIAGMLQFKPIFLGKVP-----RLVPCA 203
           +R  ++ F+  +GHK  PS+ +VP  DPT+L   AGM Q+KPIFLG          +  A
Sbjct: 64  VRDTYVDFFRGKGHKFWPSSPVVPHNDPTLLFANAGMNQYKPIFLGTADPNTELSKLSRA 123

Query: 204 ATAQRCIRT----NDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIKWAWELTTIEFGLP 263
              Q+CIR     ND+++VG+   HHTFFEMLGN+SFGDYFKKEAI+WAWEL T  +GLP
Sbjct: 124 CNTQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKKEAIEWAWELLTKVYGLP 183

Query: 264 ANRLWISIY--------EEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGPCGPCS 323
            +R++ + +        + D+EA  IW   +  P  R+   G  DNFW  G TGPCGPC+
Sbjct: 184 TDRIYATYFGGDEKAGLQPDNEARDIWLKVL--PSGRVLPFGCKDNFWEMGDTGPCGPCT 243

Query: 324 EIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMA 383
           EI+YD    +  + +   DD   +E +NLVF+Q+N++ DGSL+PL  K++DTG+G ER+ 
Sbjct: 244 EIHYDRIGNRDAASLVNNDDPTCLEIWNLVFIQFNRESDGSLKPLPAKHVDTGMGFERLT 303

Query: 384 RILQKVPNNYETDLIYPI---IQKASELAHVTYDTAD---NQSKTSLKIIGDHMRAIVYL 443
            +LQ   +NY+TD+  PI   IQKA+     +        ++   + +++ DH+R + + 
Sbjct: 304 SVLQNKMSNYDTDVFMPIFDDIQKATGARPYSGKVGPEDVDRVDMAYRVVADHIRTLSFA 363

Query: 444 ISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHID 503
           I+DG  P N GR Y++RR++RR VR G+ +    +G       F   +   VI +   + 
Sbjct: 364 IADGSRPGNEGREYVLRRILRRAVRYGKEILKAEEG-------FFNGLVSSVIRVMGDVF 423

Query: 504 EDVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLL 563
            ++K    +I + +K EE  F +TL +G         +    A +  +   L+G DAF+L
Sbjct: 424 TELKEHEKKITDIIKEEEASFCKTLAKG--------IEKFRKAGQAVQGNTLSGDDAFIL 483

Query: 564 YDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHN-----AVKLEVGEGADI 623
           +DTYGFP+++T  +A+ERG++VD+ GF+  M+  R +S++A N     A+ ++    + +
Sbjct: 484 WDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNKQAGGAIVMDADATSTL 543

Query: 624 ME-NISDT-EFLGYDMLSAKAIVESLIVNGKSVLQVSEGHD-VEVLLNRTPFYAESGGQI 683
            +  +S T +   Y            I  G + L+ S   D V ++L  T FYAE GGQI
Sbjct: 544 HKAGVSATDDSFKYIWFQDHESELKAIYTGSTFLESSAASDNVGLVLGSTSFYAEQGGQI 603

Query: 684 GDHGFIYISEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQ--GVLEVGREVKAEVDAKLR 743
            D G I  S G         V +VQ   G + +H G + +  G + VG +V  +VD + R
Sbjct: 604 FDTGLIEGSFG------TFNVCNVQIFGGFV-LHIGYLSKETGEVSVGDKVICKVDYERR 663

Query: 744 Q-------GAKVTLFACQET-----SQAGSLVAFDRLRFDFNYHRPLADTEILQIEELIN 803
           +          +  +A +E       Q GS+V  ++LRFDF++ +P+   ++ +IE ++N
Sbjct: 664 KLIAPNHTCTHMLNYALKEVLGDHIDQKGSIVLPEKLRFDFSHGKPVDPEDLRRIESIVN 723

Query: 804 GWIGDAVLLQTKVMPLAEAKS-AGAIAMFGEKYGEEVRVVEVPG---------------- 850
             I D + + +K   L+EAK   G  A+FGE Y + VRVV +                  
Sbjct: 724 KQIKDELDVFSKEAVLSEAKRIKGLRAVFGEVYPDPVRVVSIGRKVEDLLADPENNEWSL 783

BLAST of Cp4.1LG05g13540 vs. TAIR10
Match: AT1G49930.1 (AT1G49930.1 BEST Arabidopsis thaliana protein match is: Alanyl-tRNA synthetase, class IIc (TAIR:AT5G22800.1))

HSP 1 Score: 51.2 bits (121), Expect = 4.2e-06
Identity = 31/72 (43.06%), Postives = 35/72 (48.61%), Query Frame = 1

Query: 886 MDDTDADSLKGAAEFLMDNLQDPAAIVLGSCPGEGKVSLVAAFTPTVVDLGVQARNFIGP 945
           MDDTDADSLK AAE L+  L DP  +VLGS P +                          
Sbjct: 1   MDDTDADSLKSAAEHLISTLDDPVVVVLGSSPEKD------------------------- 45

Query: 946 IAKLCGGGGGGR 958
             KLCGGGGGG+
Sbjct: 61  --KLCGGGGGGK 45

BLAST of Cp4.1LG05g13540 vs. NCBI nr
Match: gi|778712781|ref|XP_004139952.2| (PREDICTED: probable alanine--tRNA ligase, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 1519.2 bits (3932), Expect = 0.0e+00
Identity = 809/1038 (77.94%), Postives = 860/1038 (82.85%), Query Frame = 1

Query: 1   MEFTLKLPNHPPRSYGRNSLTPLQRSSLYGNKPASLVRPRRKVTCSSGYKLISIILSVED 60
           MEF LKLPNH P ++   SLT LQ S+L+G+K AS+  PRRK+  SSGY           
Sbjct: 1   MEFNLKLPNHLPSTFPHISLTSLQPSTLFGHKHASIAPPRRKLMFSSGY----------- 60

Query: 61  CCERSPTLAISVKGVTIKCYTFFKLYYKEFCPFSVHHGLSQTFSSKRSSGHTTQNKKYIT 120
              R+P    S    T  C+ +F L                    K + G  TQ K   T
Sbjct: 61  -ITRNPASFPS----TRHCHRYFLL--------------------KEARG--TQLKTQ-T 120

Query: 121 SVKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGML 180
           SVKPVNEELVEDKSKDL VSGDSIR+RFLQFYASRGHKVLPSASLVPEDPTVLLTIAGML
Sbjct: 121 SVKPVNEELVEDKSKDLPVSGDSIRQRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGML 180

Query: 181 QFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK 240
           QFK +FLGKVPRLVPCAAT+QRC+RTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK
Sbjct: 181 QFKSVFLGKVPRLVPCAATSQRCLRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK 240

Query: 241 WAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGP 300
           WAWELTT+EFGLPANRLWISIYEEDDEAFAIWHDEVGVP++RIKRMGEDDNFWTSGITGP
Sbjct: 241 WAWELTTVEFGLPANRLWISIYEEDDEAFAIWHDEVGVPIDRIKRMGEDDNFWTSGITGP 300

Query: 301 CGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLG 360
           CGPCSEIYYDFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLG
Sbjct: 301 CGPCSEIYYDFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLG 360

Query: 361 LERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLI 420
           LERMARILQKVPNNYETDLIYPIIQKASELA+VTYDTADN+SKT+LKIIGDHMRA+VYLI
Sbjct: 361 LERMARILQKVPNNYETDLIYPIIQKASELANVTYDTADNRSKTNLKIIGDHMRAVVYLI 420

Query: 421 SDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDE 480
           SDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALS+HIDE
Sbjct: 421 SDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPVIAEKVIALSNHIDE 480

Query: 481 DVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLY 540
           DVKGR GRILEELKREELRFVQTLERGEKLLEEMLADALASA + GR PCLAGKDAFLLY
Sbjct: 481 DVKGRAGRILEELKREELRFVQTLERGEKLLEEMLADALASANDGGRIPCLAGKDAFLLY 540

Query: 541 DTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISD 600
           DTYGFPVEI+TEVAQERGVVVDM+GFDIEMDNQRRQSQAAHN VKLEVGEGADIMENISD
Sbjct: 541 DTYGFPVEISTEVAQERGVVVDMEGFDIEMDNQRRQSQAAHNVVKLEVGEGADIMENISD 600

Query: 601 TEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYIS 660
           TEFLGYD LSAKAIVESLIVNG  VLQVSEG+DVEVLLNRTPFYAESGGQIGDHGFIYIS
Sbjct: 601 TEFLGYDTLSAKAIVESLIVNGNPVLQVSEGNDVEVLLNRTPFYAESGGQIGDHGFIYIS 660

Query: 661 EGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFAC- 720
           EGEN  NVV+EVKDVQKSAGSIFVHKG IKQG+LEVGREV+A VDA+LRQGAKV   A  
Sbjct: 661 EGENSQNVVVEVKDVQKSAGSIFVHKGIIKQGILEVGREVEAAVDAELRQGAKVHHTATH 720

Query: 721 -----------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQT 780
                      QETSQAGSLVAFDRLRFDFNYHRPL D EI++IEELINGWIGDAVLLQT
Sbjct: 721 LLQSALKRIIGQETSQAGSLVAFDRLRFDFNYHRPLVDAEIVKIEELINGWIGDAVLLQT 780

Query: 781 KVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGI 840
           KVM L EAK AGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQGI
Sbjct: 781 KVMALTEAKKAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQGI 840

Query: 841 ASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRV------- 900
           ASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRV+NL+E+LR+       
Sbjct: 841 ASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVDNLLEELRMARNEISN 900

Query: 901 LVEYMDDTDADSLKGAA---------EFLMDNLQDPAAIVLGSCPG------EGKVSLVA 960
           L E      A S+   A           L++ + D  A  L S         +  V++V 
Sbjct: 901 LREKTAVAKASSIANKAFVVGTSKEIRVLVEYMDDADADSLKSAAEFLMDNLQDPVAIVL 960

Query: 961 AFTPTVVDLGVQAR-------------NFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLN 992
              P    + + A               FIG IAKLCGGGGGGRPNFAQAGGRKPENLL+
Sbjct: 961 GSCPGEGKVSLVAAFTPSVVDLGVQAGKFIGSIAKLCGGGGGGRPNFAQAGGRKPENLLD 999

BLAST of Cp4.1LG05g13540 vs. NCBI nr
Match: gi|659094957|ref|XP_008448323.1| (PREDICTED: probable alanine--tRNA ligase, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 1478.8 bits (3827), Expect = 0.0e+00
Identity = 770/919 (83.79%), Postives = 804/919 (87.49%), Query Frame = 1

Query: 120 TSVKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGM 179
           TSVKPVNEELVEDKSKDL VSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGM
Sbjct: 81  TSVKPVNEELVEDKSKDLPVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGM 140

Query: 180 LQFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAI 239
           LQFKP+FLGKVPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAI
Sbjct: 141 LQFKPVFLGKVPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAI 200

Query: 240 KWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITG 299
           KWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPV+RIKRMGEDDNFWTSGITG
Sbjct: 201 KWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEDDNFWTSGITG 260

Query: 300 PCGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGL 359
           PCGPCSEIYYDFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNK+DDGSLEPLKQKNIDTGL
Sbjct: 261 PCGPCSEIYYDFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKRDDGSLEPLKQKNIDTGL 320

Query: 360 GLERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYL 419
           GLERMARILQKVPNNYETDLIYPIIQKASELA+VTYDTADNQSKT+LKIIGDHMRA+VYL
Sbjct: 321 GLERMARILQKVPNNYETDLIYPIIQKASELANVTYDTADNQSKTNLKIIGDHMRAVVYL 380

Query: 420 ISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHID 479
           ISDGVVPSNIGRGY+VRRLIRRVVRTGRLLGIKGDG GN D AFTP IAEKVIALS+HID
Sbjct: 381 ISDGVVPSNIGRGYVVRRLIRRVVRTGRLLGIKGDGIGNIDRAFTPLIAEKVIALSNHID 440

Query: 480 EDVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLL 539
           EDVKGR GRILEELKREELRFVQTLERGEKLLEEMLADALASAKE GR PCLAGKDAFLL
Sbjct: 441 EDVKGRAGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEGGRIPCLAGKDAFLL 500

Query: 540 YDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENIS 599
           YDTYGFPVEITTEVAQERGVVVDM+GFDIEMDNQRRQSQAAHN VKLEVGEGADIME IS
Sbjct: 501 YDTYGFPVEITTEVAQERGVVVDMEGFDIEMDNQRRQSQAAHNVVKLEVGEGADIMETIS 560

Query: 600 DTEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYI 659
           DTEFLGYD LSAKAIVESLIVNG  VLQVSEG+DVEVLLNRTPFYAESGGQIGDHGFIYI
Sbjct: 561 DTEFLGYDTLSAKAIVESLIVNGNPVLQVSEGNDVEVLLNRTPFYAESGGQIGDHGFIYI 620

Query: 660 SEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFAC 719
           SEGEN  NVV+EVKDVQKSAGSIFVHKG IKQG+LEVGREV+A VDA+LRQ AKV   A 
Sbjct: 621 SEGENSQNVVVEVKDVQKSAGSIFVHKGIIKQGILEVGREVEAAVDAELRQRAKVHHTAT 680

Query: 720 ------------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQ 779
                       QETSQAGSLVAFDRLRFDFNYHRPL D EI++IEELINGWIGDAVLLQ
Sbjct: 681 HLLQSALKRTIGQETSQAGSLVAFDRLRFDFNYHRPLVDAEIVKIEELINGWIGDAVLLQ 740

Query: 780 TKVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQG 839
           TKVMPL EAK AGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQG
Sbjct: 741 TKVMPLTEAKKAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQG 800

Query: 840 IASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRV------ 899
           IASGVRRIEAVAGDAFIEYVN RD HMKRLCTMLKVKAEDVTTRV+NL+E+LR+      
Sbjct: 801 IASGVRRIEAVAGDAFIEYVNTRDSHMKRLCTMLKVKAEDVTTRVDNLLEELRMARNEIS 860

Query: 900 -LVEYMDDTDADSLKGAA---------EFLMDNLQDPAAIVLGSCPG------EGKVSLV 959
            L E      A S+   A           L++ + D  A  L S         +   ++V
Sbjct: 861 NLREKTAVFKASSIANKAFLVGTSKEIRVLVEYMDDTDADSLKSAAEFLIDNLQDPAAIV 920

Query: 960 AAFTPTVVDLGVQAR-------------NFIGPIAKLCGGGGGGRPNFAQAGGRKPENLL 992
               P    + + A               FIG IAKLCGGGGGGRPNFAQAGGRKPENLL
Sbjct: 921 LGSCPGEGKVSLVAAFTPSVVDLGVQAGKFIGSIAKLCGGGGGGRPNFAQAGGRKPENLL 980

BLAST of Cp4.1LG05g13540 vs. NCBI nr
Match: gi|702464048|ref|XP_010028807.1| (PREDICTED: probable alanine--tRNA ligase, chloroplastic isoform X1 [Eucalyptus grandis])

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 694/918 (75.60%), Postives = 780/918 (84.97%), Query Frame = 1

Query: 121 SVKPVNEELVEDKSKDLQVSGDSIRRRFLQFYASRGHKVLPSASLVPEDPTVLLTIAGML 180
           SV+PV EEL+E+KS DL VSGDSIRRRFL FYASRGHKVLPSASLVP+DPTVLLTIAGML
Sbjct: 68  SVQPVTEELIEEKSNDLLVSGDSIRRRFLDFYASRGHKVLPSASLVPDDPTVLLTIAGML 127

Query: 181 QFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK 240
           QFKPIFLGKVPR VPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK
Sbjct: 128 QFKPIFLGKVPRQVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEAIK 187

Query: 241 WAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGITGP 300
           WAWEL+T E GL A RLWIS++E+DDEAF IWH EVGVP + IKRMGE+DNFWTSG+TGP
Sbjct: 188 WAWELSTAELGLQAERLWISVFEDDDEAFEIWHKEVGVPADHIKRMGEEDNFWTSGVTGP 247

Query: 301 CGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLG 360
           CGPCSEIYYDF PE+G  D DLGDD+RF+EFYNLVFMQYNKKDDGSLEPLKQKNIDTG+G
Sbjct: 248 CGPCSEIYYDFHPERGTLDTDLGDDSRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGMG 307

Query: 361 LERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVYLI 420
           LER+ARILQ+VP+NYETDLIYPII+KASELA+V+Y  AD+ +K  LKIIGDHMRAIVYLI
Sbjct: 308 LERVARILQRVPSNYETDLIYPIIEKASELANVSYGLADDPTKLKLKIIGDHMRAIVYLI 367

Query: 421 SDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHIDE 480
           SDGV+PSNIGRGY+VRRLIRRVVRTGRLLGI+GD + N +GAF P IAEKVI   +HID 
Sbjct: 368 SDGVIPSNIGRGYVVRRLIRRVVRTGRLLGIRGDCRENLEGAFLPAIAEKVIDFGTHIDS 427

Query: 481 DVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFLLY 540
           DVK R  RILEE+KREE RFVQTLERGE+LLE+M+ DAL+S +E G  PCLAG+DAFLLY
Sbjct: 428 DVKARAPRILEEIKREEYRFVQTLERGEELLEQMIDDALSSTREKGTVPCLAGEDAFLLY 487

Query: 541 DTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENISD 600
           DTYGFPVEIT EVA+E GV VDM  FD EM+ QRR+SQAAH+A+KLEVG+ ADI  NI D
Sbjct: 488 DTYGFPVEITIEVAEEHGVTVDMNAFDTEMEKQRRRSQAAHHAIKLEVGDDADITANIPD 547

Query: 601 TEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIYIS 660
           TEFLGYD LSA+A+++SL+VNGKSV+QV EG +VEVLL++TPFYAESGGQI DHG + +S
Sbjct: 548 TEFLGYDTLSARAVIKSLLVNGKSVIQVPEGSEVEVLLDKTPFYAESGGQIADHGLLCVS 607

Query: 661 EGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFAC- 720
           +GEN+   V+E+ DVQKS G++FVHKGTIK+GVLEVG EV+AEVD KLRQ AKV   A  
Sbjct: 608 DGENQQRTVVEIMDVQKSMGNLFVHKGTIKEGVLEVGIEVEAEVDPKLRQRAKVHHTATH 667

Query: 721 -----------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLLQT 780
                      QETSQAGSLVAFDRLRFDFN+HRPL + EIL+IE LINGWIGDA +LQT
Sbjct: 668 LLQSALKKVIGQETSQAGSLVAFDRLRFDFNFHRPLLEDEILEIERLINGWIGDAAVLQT 727

Query: 781 KVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQGI 840
           KVMPL EAK AGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHV NT+EIRGFK+ISEQGI
Sbjct: 728 KVMPLTEAKEAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVSNTAEIRGFKIISEQGI 787

Query: 841 ASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENL------------- 900
           ASG+RRIEAVAG+AFIEYVN+RDY+MK+LC  LKVKAE+VTTRV+NL             
Sbjct: 788 ASGIRRIEAVAGEAFIEYVNSRDYYMKQLCNNLKVKAEEVTTRVDNLLEELRMARNEVSL 847

Query: 901 ----------------------VEDLRVLVEYMDDTDADSLKGAAEFLMDNLQDPAAIVL 960
                                  +++RVLVE M+DTDADSLK AAE+L+D+LQDPAA+VL
Sbjct: 848 LRSKAAVYKASTIASKAVLVGSSKEIRVLVESMEDTDADSLKSAAEYLIDSLQDPAAVVL 907

Query: 961 GSCPGEGKVSLVAAFTPTVVDLGVQARNFIGPIAKLCGGGGGGRPNFAQAGGRKPENLLN 992
           GS P EGKVSLVAAFTP VV LG+QA  FIGPIAKLCGGGGGGRPNFAQAGGRKPENL +
Sbjct: 908 GSSPSEGKVSLVAAFTPGVVKLGIQAGKFIGPIAKLCGGGGGGRPNFAQAGGRKPENLSS 967

BLAST of Cp4.1LG05g13540 vs. NCBI nr
Match: gi|778712785|ref|XP_011656934.1| (PREDICTED: probable alanine--tRNA ligase, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 1374.0 bits (3555), Expect = 0.0e+00
Identity = 711/860 (82.67%), Postives = 750/860 (87.21%), Query Frame = 1

Query: 179 MLQFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEA 238
           MLQFK +FLGKVPRLVPCAAT+QRC+RTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEA
Sbjct: 1   MLQFKSVFLGKVPRLVPCAATSQRCLRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEA 60

Query: 239 IKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGIT 298
           IKWAWELTT+EFGLPANRLWISIYEEDDEAFAIWHDEVGVP++RIKRMGEDDNFWTSGIT
Sbjct: 61  IKWAWELTTVEFGLPANRLWISIYEEDDEAFAIWHDEVGVPIDRIKRMGEDDNFWTSGIT 120

Query: 299 GPCGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTG 358
           GPCGPCSEIYYDFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTG
Sbjct: 121 GPCGPCSEIYYDFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTG 180

Query: 359 LGLERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVY 418
           LGLERMARILQKVPNNYETDLIYPIIQKASELA+VTYDTADN+SKT+LKIIGDHMRA+VY
Sbjct: 181 LGLERMARILQKVPNNYETDLIYPIIQKASELANVTYDTADNRSKTNLKIIGDHMRAVVY 240

Query: 419 LISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHI 478
           LISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDG+GN DGAFTP IAEKVIALS+HI
Sbjct: 241 LISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGRGNIDGAFTPVIAEKVIALSNHI 300

Query: 479 DEDVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFL 538
           DEDVKGR GRILEELKREELRFVQTLERGEKLLEEMLADALASA + GR PCLAGKDAFL
Sbjct: 301 DEDVKGRAGRILEELKREELRFVQTLERGEKLLEEMLADALASANDGGRIPCLAGKDAFL 360

Query: 539 LYDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENI 598
           LYDTYGFPVEI+TEVAQERGVVVDM+GFDIEMDNQRRQSQAAHN VKLEVGEGADIMENI
Sbjct: 361 LYDTYGFPVEISTEVAQERGVVVDMEGFDIEMDNQRRQSQAAHNVVKLEVGEGADIMENI 420

Query: 599 SDTEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIY 658
           SDTEFLGYD LSAKAIVESLIVNG  VLQVSEG+DVEVLLNRTPFYAESGGQIGDHGFIY
Sbjct: 421 SDTEFLGYDTLSAKAIVESLIVNGNPVLQVSEGNDVEVLLNRTPFYAESGGQIGDHGFIY 480

Query: 659 ISEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFA 718
           ISEGEN  NVV+EVKDVQKSAGSIFVHKG IKQG+LEVGREV+A VDA+LRQGAKV   A
Sbjct: 481 ISEGENSQNVVVEVKDVQKSAGSIFVHKGIIKQGILEVGREVEAAVDAELRQGAKVHHTA 540

Query: 719 C------------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLL 778
                        QETSQAGSLVAFDRLRFDFNYHRPL D EI++IEELINGWIGDAVLL
Sbjct: 541 THLLQSALKRIIGQETSQAGSLVAFDRLRFDFNYHRPLVDAEIVKIEELINGWIGDAVLL 600

Query: 779 QTKVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQ 838
           QTKVM L EAK AGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQ
Sbjct: 601 QTKVMALTEAKKAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQ 660

Query: 839 GIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRV----- 898
           GIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRV+NL+E+LR+     
Sbjct: 661 GIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVDNLLEELRMARNEI 720

Query: 899 --LVEYMDDTDADSLKGAA---------EFLMDNLQDPAAIVLGSCPG------EGKVSL 958
             L E      A S+   A           L++ + D  A  L S         +  V++
Sbjct: 721 SNLREKTAVAKASSIANKAFVVGTSKEIRVLVEYMDDADADSLKSAAEFLMDNLQDPVAI 780

Query: 959 VAAFTPTVVDLGVQAR-------------NFIGPIAKLCGGGGGGRPNFAQAGGRKPENL 992
           V    P    + + A               FIG IAKLCGGGGGGRPNFAQAGGRKPENL
Sbjct: 781 VLGSCPGEGKVSLVAAFTPSVVDLGVQAGKFIGSIAKLCGGGGGGRPNFAQAGGRKPENL 840

BLAST of Cp4.1LG05g13540 vs. NCBI nr
Match: gi|659094959|ref|XP_008448324.1| (PREDICTED: probable alanine--tRNA ligase, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 1368.6 bits (3541), Expect = 0.0e+00
Identity = 712/860 (82.79%), Postives = 746/860 (86.74%), Query Frame = 1

Query: 179 MLQFKPIFLGKVPRLVPCAATAQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEA 238
           MLQFKP+FLGKVPRLVPCAAT+QRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEA
Sbjct: 1   MLQFKPVFLGKVPRLVPCAATSQRCIRTNDVENVGRTARHHTFFEMLGNFSFGDYFKKEA 60

Query: 239 IKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVERIKRMGEDDNFWTSGIT 298
           IKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPV+RIKRMGEDDNFWTSGIT
Sbjct: 61  IKWAWELTTIEFGLPANRLWISIYEEDDEAFAIWHDEVGVPVDRIKRMGEDDNFWTSGIT 120

Query: 299 GPCGPCSEIYYDFQPEKGHSDVDLGDDTRFMEFYNLVFMQYNKKDDGSLEPLKQKNIDTG 358
           GPCGPCSEIYYDFQPEKG+SDVDLGDDTRFMEFYNLVFMQYNK+DDGSLEPLKQKNIDTG
Sbjct: 121 GPCGPCSEIYYDFQPEKGYSDVDLGDDTRFMEFYNLVFMQYNKRDDGSLEPLKQKNIDTG 180

Query: 359 LGLERMARILQKVPNNYETDLIYPIIQKASELAHVTYDTADNQSKTSLKIIGDHMRAIVY 418
           LGLERMARILQKVPNNYETDLIYPIIQKASELA+VTYDTADNQSKT+LKIIGDHMRA+VY
Sbjct: 181 LGLERMARILQKVPNNYETDLIYPIIQKASELANVTYDTADNQSKTNLKIIGDHMRAVVY 240

Query: 419 LISDGVVPSNIGRGYIVRRLIRRVVRTGRLLGIKGDGKGNTDGAFTPHIAEKVIALSSHI 478
           LISDGVVPSNIGRGY+VRRLIRRVVRTGRLLGIKGDG GN D AFTP IAEKVIALS+HI
Sbjct: 241 LISDGVVPSNIGRGYVVRRLIRRVVRTGRLLGIKGDGIGNIDRAFTPLIAEKVIALSNHI 300

Query: 479 DEDVKGRTGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEDGRFPCLAGKDAFL 538
           DEDVKGR GRILEELKREELRFVQTLERGEKLLEEMLADALASAKE GR PCLAGKDAFL
Sbjct: 301 DEDVKGRAGRILEELKREELRFVQTLERGEKLLEEMLADALASAKEGGRIPCLAGKDAFL 360

Query: 539 LYDTYGFPVEITTEVAQERGVVVDMKGFDIEMDNQRRQSQAAHNAVKLEVGEGADIMENI 598
           LYDTYGFPVEITTEVAQERGVVVDM+GFDIEMDNQRRQSQAAHN VKLEVGEGADIME I
Sbjct: 361 LYDTYGFPVEITTEVAQERGVVVDMEGFDIEMDNQRRQSQAAHNVVKLEVGEGADIMETI 420

Query: 599 SDTEFLGYDMLSAKAIVESLIVNGKSVLQVSEGHDVEVLLNRTPFYAESGGQIGDHGFIY 658
           SDTEFLGYD LSAKAIVESLIVNG  VLQVSEG+DVEVLLNRTPFYAESGGQIGDHGFIY
Sbjct: 421 SDTEFLGYDTLSAKAIVESLIVNGNPVLQVSEGNDVEVLLNRTPFYAESGGQIGDHGFIY 480

Query: 659 ISEGENRGNVVMEVKDVQKSAGSIFVHKGTIKQGVLEVGREVKAEVDAKLRQGAKVTLFA 718
           ISEGEN  NVV+EVKDVQKSAGSIFVHKG IKQG+LEVGREV+A VDA+LRQ AKV   A
Sbjct: 481 ISEGENSQNVVVEVKDVQKSAGSIFVHKGIIKQGILEVGREVEAAVDAELRQRAKVHHTA 540

Query: 719 C------------QETSQAGSLVAFDRLRFDFNYHRPLADTEILQIEELINGWIGDAVLL 778
                        QETSQAGSLVAFDRLRFDFNYHRPL D EI++IEELINGWIGDAVLL
Sbjct: 541 THLLQSALKRTIGQETSQAGSLVAFDRLRFDFNYHRPLVDAEIVKIEELINGWIGDAVLL 600

Query: 779 QTKVMPLAEAKSAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKVISEQ 838
           QTKVMPL EAK AGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFK+ISEQ
Sbjct: 601 QTKVMPLTEAKKAGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVFNTSEIRGFKIISEQ 660

Query: 839 GIASGVRRIEAVAGDAFIEYVNARDYHMKRLCTMLKVKAEDVTTRVENLVEDLRV----- 898
           GIASGVRRIEAVAGDAFIEYVN RD HMKRLCTMLKVKAEDVTTRV+NL+E+LR+     
Sbjct: 661 GIASGVRRIEAVAGDAFIEYVNTRDSHMKRLCTMLKVKAEDVTTRVDNLLEELRMARNEI 720

Query: 899 --LVEYMDDTDADSLKGAA---------EFLMDNLQDPAAIVLGSCPG------EGKVSL 958
             L E      A S+   A           L++ + D  A  L S         +   ++
Sbjct: 721 SNLREKTAVFKASSIANKAFLVGTSKEIRVLVEYMDDTDADSLKSAAEFLIDNLQDPAAI 780

Query: 959 VAAFTPTVVDLGVQAR-------------NFIGPIAKLCGGGGGGRPNFAQAGGRKPENL 992
           V    P    + + A               FIG IAKLCGGGGGGRPNFAQAGGRKPENL
Sbjct: 781 VLGSCPGEGKVSLVAAFTPSVVDLGVQAGKFIGSIAKLCGGGGGGRPNFAQAGGRKPENL 840