Cp4.1LG05g13220 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG05g13220 vs. Swiss-Prot
Match: CLCE_ARATH (Chloride channel protein CLC-e OS=Arabidopsis thaliana GN=CLC-E PE=2 SV=2)

HSP 1 Score: 734.6 bits (1895), Expect = 1.1e-210
Identity = 409/661 (61.88%), Postives = 490/661 (74.13%), Query Frame = 1

Query: 108 EEDEEEEGIPSGIGSS-TIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLR 167
           E D++E G          I S+C VGVLTG+ VVLFNN VH LRDF WDGIP+RGASWLR
Sbjct: 59  ETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRGASWLR 118

Query: 168 EMPVEETWKRVILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKA 227
           E P+   W RVILVP  GG +VS LN LRE+         G   S  ++ +A L+PFLK 
Sbjct: 119 EAPIGSNWLRVILVPTIGGLVVSILNQLRESAGKST----GDSHSSLDRVKAVLRPFLKT 178

Query: 228 VAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAA 287
           VAA VTLGTGNSLGPEGPSV+IG S+ KGV+++F+++ +T  SL+AAGSAAGISSGFNAA
Sbjct: 179 VAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLFNKSPQTGFSLLAAGSAAGISSGFNAA 238

Query: 288 VAGCFFAVESVLWPSPA-DSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFR 347
           VAGCFFAVESVLWPS + DS+ SL NTTSMVILSAV AS VS++GLG EPAFKVP+YDFR
Sbjct: 239 VAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFR 298

Query: 348 SPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIAL 407
           SP ELPLYLLLGALCGLVSL+ S+CTS M + VD ++KD G+ +A+FP++GG S G+IAL
Sbjct: 299 SPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIAL 358

Query: 408 AYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLF 467
            YPE+LYWGF+NVD+LLE RPFVK LSA+LL QLV VKI AT+ CRASGLVGGYYAPSLF
Sbjct: 359 VYPEVLYWGFQNVDILLEKRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLF 418

Query: 468 IGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 527
           IG A GMAYGKFIG+AL++ N   + SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLL
Sbjct: 419 IGGAAGMAYGKFIGLALAQ-NPDFNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 478

Query: 528 FELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQ 587
           FELTQDYRIVLPLLGAVG+SSW+TSGQ +++ +++TK+    K     QS T   +++D+
Sbjct: 479 FELTQDYRIVLPLLGAVGMSSWITSGQSKRQETRETKETRKRKSQEAVQSLT---SSDDE 538

Query: 588 SSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIE 647
           SS           N+LCE+ESSLC  DS ++  EL + I VSEAM TR+ TV M T L E
Sbjct: 539 SST----------NNLCEVESSLCLDDSLNQSEELPKSIFVSEAMRTRFATVMMSTSLEE 598

Query: 648 AVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSK---------NGLVVSEVCSLDGEI 707
           A+  ML EKQS ALIVD +N  +GIL L DIQ+ SK           + V+++CS  G  
Sbjct: 599 ALTRMLIEKQSCALIVDPDNIFLGILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSGGK 658

Query: 708 CRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMG----YLVGVLDLECIDLTCRILAT 754
           C+VPWT TP MD+L A+T+M    +S V VV   +     + VGVLD ECI LT R LAT
Sbjct: 659 CKVPWTVTPDMDLLAAQTIMNKHELSHVAVVSGSIDAPRIHPVGVLDRECITLTRRALAT 701

BLAST of Cp4.1LG05g13220 vs. Swiss-Prot
Match: CLCF_ARATH (Chloride channel protein CLC-f OS=Arabidopsis thaliana GN=CLC-F PE=2 SV=2)

HSP 1 Score: 410.6 bits (1054), Expect = 3.7e-113
Identity = 281/700 (40.14%), Postives = 398/700 (56.86%), Query Frame = 1

Query: 46  RFSLRPKQTGFRFRSFCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEED 105
           RFSL  ++  F+     ++    E  +P   SS   FS   G     GI      ++  D
Sbjct: 59  RFSLSGRRLSFKRME--NIRVDRERHNP---SSSSAFSAA-GEEDGGGISNLHSVDDRND 118

Query: 106 NTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWL 165
               DEE  G  +    + ++  C +GV  GI V  FN  VH + ++ W G PN GA+WL
Sbjct: 119 EYGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAWL 178

Query: 166 REMPVEETWKRVILVPACGGFLVSFLNLLREATD----NDPSTKLGVPVSISNQFRAALQ 225
           R   + +TW R++L+P  GG +V  ++ L E  D    ++ S + G+       F A + 
Sbjct: 179 RLQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGL------DFLAGIY 238

Query: 226 PFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISS 285
           P +KA+ A+VTLGTG SLGPEGPSVDIG S   G + + + N   +++L AAG+A+GI+S
Sbjct: 239 PVIKAIQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRERRIALTAAGAASGIAS 298

Query: 286 GFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPE 345
           GFNAAVAGCFFA+E+VL P  A++  S   TT+M+IL++VI+S VS   LG + AF VP 
Sbjct: 299 GFNAAVAGCFFAIETVLRPLRAEN--SPPFTTAMIILASVISSTVSNALLGTQSAFTVPS 358

Query: 346 YDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTG 405
           YD +S +ELPLYL+LG LCG VS+ FS+  ++   + D +   FG+   + P LGG   G
Sbjct: 359 YDLKSAAELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAG 418

Query: 406 LIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYA 465
           +IAL YP ILYWGF NV+ +L +          LLAQL   K++AT+LC+ SGLVGG YA
Sbjct: 419 IIALKYPGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYA 478

Query: 466 PSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTA 525
           PSL IGAA G  +G   G A    N AI  +   VA PQAY LVGMAATLA +C VPLT+
Sbjct: 479 PSLMIGAAVGAVFG---GSAAEIINRAIPGNA-AVAQPQAYALVGMAATLASMCSVPLTS 538

Query: 526 VLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSN 585
           VLLLFELT+DYRI+LPL+GAVG++ W+ S   + + S  ++    G+  S+   +   + 
Sbjct: 539 VLLLFELTKDYRILLPLMGAVGLAIWVPSVANQGKESDSSEGRSTGRGYSSLSPSERKTE 598

Query: 586 ANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDT 645
              + ++ AD  + T    + E        D ++ + +L+    V   M+  YV V   T
Sbjct: 599 GVWRHTDNADSLELT----VIENPDHNSFLDEETILEDLK----VMRVMSKNYVKVSSGT 658

Query: 646 FLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQK---------LSKNGLVVSEVC-- 705
            L EA +++    Q+  ++VD+++ L GIL   DI++         L +N   VS VC  
Sbjct: 659 TLREARNILKESHQNCIMVVDDDDFLAGILTHGDIRRYLSNNASTILDENTCPVSSVCTK 718

Query: 706 --SLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVK 729
             S  G+  R   T  P   +  AK +M+  GV Q+PVVK
Sbjct: 719 KISYRGQE-RGLLTCYPDATVGVAKELMEARGVKQLPVVK 731

BLAST of Cp4.1LG05g13220 vs. Swiss-Prot
Match: CLCA_VIBC3 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) GN=clcA PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.9e-32
Identity = 131/437 (29.98%), Postives = 202/437 (46.22%), Query Frame = 1

Query: 123 STIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILVPA 182
           S +  S  VG+L G+    F  AVH + +   D + +   S+L        W    L+ A
Sbjct: 32  SVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFL------PLWLAAFLISA 91

Query: 183 CGGFLVSFL--NLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLG 242
              F+  FL      EA  +      G    +       + P +K       LG+G  LG
Sbjct: 92  FLAFIGYFLVHRFAPEAAGSGIPEIEGAMDGMRPVRWWRVLP-VKFFGGMGALGSGMVLG 151

Query: 243 PEGPSVDIGTSVGKGVSTVFD-RNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 302
            EGP+V +G +VG+ +S +F  +N  T+ SL+AAG+A G+++ FNA +AG  F +E +  
Sbjct: 152 REGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAAGGLAAAFNAPLAGIMFVIEEMR- 211

Query: 303 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 362
                  ++L +  + VI+SAV A+ V +V  G +    +P+YD    S L L+LLLGAL
Sbjct: 212 ---PQFRYTLISVRA-VIISAVAANIVFRVINGQDAVITMPQYDAPELSTLGLFLLLGAL 271

Query: 363 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 422
            G+  + F+   +       K H++      L   + G   GL+ L  PE+   G   + 
Sbjct: 272 FGVFGVLFNYLITLAQDLFVKFHRNDRKRYLLTGSMIGGCFGLLLLYVPELTGGGISLIP 331

Query: 423 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 482
            +           A +L  L V +I  T LC  SG  GG +AP L +G   G A+G    
Sbjct: 332 TITNGG-----YGAGILLLLFVGRIFTTLLCFGSGAPGGIFAPMLALGTLFGYAFGLIAK 391

Query: 483 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPL- 542
           +   E N            P  + + GM A  A   + P+T +LL+ E+T +Y ++LPL 
Sbjct: 392 VWFPELNI----------EPGMFAIAGMGALFAATVRAPITGILLVIEMTNNYHLILPLI 440

Query: 543 ---LGAVGVSSWLTSGQ 553
              LGAV + + L  GQ
Sbjct: 452 ITSLGAV-IFAQLLGGQ 440

BLAST of Cp4.1LG05g13220 vs. Swiss-Prot
Match: CLCA_VIBCH (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) GN=clcA PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 3.2e-32
Identity = 131/437 (29.98%), Postives = 202/437 (46.22%), Query Frame = 1

Query: 123 STIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILVPA 182
           S +  S  VG+L G+    F  AVH + +   D + +   S+L        W    L+ A
Sbjct: 32  SVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFL------PLWLAAFLISA 91

Query: 183 CGGFLVSFL--NLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLG 242
              F+  FL      EA  +      G    +       + P +K       LG+G  LG
Sbjct: 92  FLAFIGYFLVHRFAPEAAGSGIPEIEGAMDGMRPVRWWRVLP-VKFFGGMGALGSGMVLG 151

Query: 243 PEGPSVDIGTSVGKGVSTVFD-RNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 302
            EGP+V +G +VG+ +S +F  +N  T+ SL+AAG+A G+++ FNA +AG  F +E +  
Sbjct: 152 REGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAAGGLAAAFNAPLAGIMFVIEEMR- 211

Query: 303 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 362
                  ++L +  + VI+SAV A+ V +V  G +    +P+YD    S L L+LLLGAL
Sbjct: 212 ---PQFRYTLISVRA-VIISAVAANIVFRVINGQDAVITMPQYDAPELSTLGLFLLLGAL 271

Query: 363 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 422
            G+  + F+   +       K H++      L   + G   GL+ L  PE+   G   + 
Sbjct: 272 FGVFGVLFNYLITLAQDLFVKFHRNDRKRYLLTGSMIGGCFGLLLLYVPELTGGGISLIP 331

Query: 423 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 482
            +           A +L  L V +I  T LC  SG  GG +AP L +G   G A+G    
Sbjct: 332 TITNGG-----YGAGILLLLFVGRIFTTLLCFGSGAPGGIFAPMLALGTLFGYAFGLIAK 391

Query: 483 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPL- 542
           +   E N            P  + + GM A  A   + P+T +LL+ E+T +Y ++LPL 
Sbjct: 392 MWFPELNI----------EPGMFAIAGMGALFAATVRAPITGILLVIEMTNNYHLILPLI 440

Query: 543 ---LGAVGVSSWLTSGQ 553
              LGAV + + L  GQ
Sbjct: 452 ITSLGAV-IFAQLLGGQ 440

BLAST of Cp4.1LG05g13220 vs. Swiss-Prot
Match: CLCA_VIBCM (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain M66-2) GN=clcA PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 3.2e-32
Identity = 131/437 (29.98%), Postives = 202/437 (46.22%), Query Frame = 1

Query: 123 STIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILVPA 182
           S +  S  VG+L G+    F  AVH + +   D + +   S+L        W    L+ A
Sbjct: 32  SVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFL------PLWLAAFLISA 91

Query: 183 CGGFLVSFL--NLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLG 242
              F+  FL      EA  +      G    +       + P +K       LG+G  LG
Sbjct: 92  FLAFIGYFLVHRFAPEAAGSGIPEIEGAMDGMRPVRWWRVLP-VKFFGGMGALGSGMVLG 151

Query: 243 PEGPSVDIGTSVGKGVSTVFD-RNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 302
            EGP+V +G +VG+ +S +F  +N  T+ SL+AAG+A G+++ FNA +AG  F +E +  
Sbjct: 152 REGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAAGGLAAAFNAPLAGIMFVIEEMR- 211

Query: 303 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 362
                  ++L +  + VI+SAV A+ V +V  G +    +P+YD    S L L+LLLGAL
Sbjct: 212 ---PQFRYTLISVRA-VIISAVAANIVFRVINGQDAVITMPQYDAPELSTLGLFLLLGAL 271

Query: 363 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 422
            G+  + F+   +       K H++      L   + G   GL+ L  PE+   G   + 
Sbjct: 272 FGVFGVLFNYLITLAQDLFVKFHRNDRKRYLLTGSMIGGCFGLLLLYVPELTGGGISLIP 331

Query: 423 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 482
            +           A +L  L V +I  T LC  SG  GG +AP L +G   G A+G    
Sbjct: 332 TITNGG-----YGAGILLLLFVGRIFTTLLCFGSGAPGGIFAPMLALGTLFGYAFGLIAK 391

Query: 483 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPL- 542
           +   E N            P  + + GM A  A   + P+T +LL+ E+T +Y ++LPL 
Sbjct: 392 MWFPELNI----------EPGMFAIAGMGALFAATVRAPITGILLVIEMTNNYHLILPLI 440

Query: 543 ---LGAVGVSSWLTSGQ 553
              LGAV + + L  GQ
Sbjct: 452 ITSLGAV-IFAQLLGGQ 440

BLAST of Cp4.1LG05g13220 vs. TrEMBL
Match: A0A0A0KAV3_CUCSA (Chloride channel protein OS=Cucumis sativus GN=Csa_6G116660 PE=3 SV=1)

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 579/677 (85.52%), Postives = 621/677 (91.73%), Query Frame = 1

Query: 98  KEEEEEEDNTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGI 157
           +EEEEEE+  EE+EEEEGIP G GSSTIISSCFVG+LTGIGVVLFNNAVHE+RDF WDGI
Sbjct: 276 EEEEEEEEEEEEEEEEEGIPFGTGSSTIISSCFVGLLTGIGVVLFNNAVHEIRDFSWDGI 335

Query: 158 PNRGASWLREMPVEETWKRVILVPACGGFLVSFLNLLREATD--------NDPSTKLGVP 217
           PNRGASWLREMP+E+ WKRVILVPA GGFLVSFLNLLR+ATD        +DPSTK GVP
Sbjct: 336 PNRGASWLREMPIEDIWKRVILVPASGGFLVSFLNLLRDATDVKVDQPQGDDPSTKFGVP 395

Query: 218 VSISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLS 277
           +SISN+ +AALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKG+STVF+ NSRTKLS
Sbjct: 396 ISISNKLKAALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFEMNSRTKLS 455

Query: 278 LIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQV 337
           LIAAGSAAGISSGFNAAVAGCFFA+ESVLWPSPADSTFSLTNTTSMVILSAVIAS VSQV
Sbjct: 456 LIAAGSAAGISSGFNAAVAGCFFAIESVLWPSPADSTFSLTNTTSMVILSAVIASVVSQV 515

Query: 338 GLGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSR 397
           GLGVEPAFKVP YDFRSPSELPLYLLLG LCGLVSLSFSKCTSY+LATVDK HK+FG  R
Sbjct: 516 GLGVEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSKCTSYLLATVDKFHKEFGAPR 575

Query: 398 ALFPILGGFSTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSL 457
           A+FPILGGF+ GLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVV+KILATSL
Sbjct: 576 AMFPILGGFTIGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVIKILATSL 635

Query: 458 CRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAA 517
           CRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPN+ +DFSIFEVASPQAYGLVGMAA
Sbjct: 636 CRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGMAA 695

Query: 518 TLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKV 577
           TLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSW+TSGQKRKRSSQ+TKKL  GK 
Sbjct: 696 TLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQQTKKLSPGKS 755

Query: 578 LSTQQSTTYDSNANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEA 637
            STQQST YDSNAN+QSSNYA+DG+  YPNDLCEIESSLCAY+SDSE VELERKI VSEA
Sbjct: 756 PSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSLCAYESDSETVELERKISVSEA 815

Query: 638 MTTRYVTVFMDTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSKNG------ 697
           MTT+Y+T+ M T L+EAV+LMLAEKQS ALIVDE N LIGIL L+DIQKLSKN       
Sbjct: 816 MTTKYITILMGTCLVEAVNLMLAEKQSCALIVDEGNILIGILELEDIQKLSKNAKSRNEQ 875

Query: 698 ---LVVSEVCSLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDL 757
               VVSE+CSLD ++CRVPWTATPSMDILTAK +MKNLGV+QVPVV+DQMGY+VGVLD 
Sbjct: 876 LKDFVVSEICSLDEKMCRVPWTATPSMDILTAKMIMKNLGVTQVPVVRDQMGYVVGVLDW 935

BLAST of Cp4.1LG05g13220 vs. TrEMBL
Match: M5VYH8_PRUPE (Chloride channel protein OS=Prunus persica GN=PRUPE_ppa025837mg PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 1.3e-237
Identity = 454/697 (65.14%), Postives = 542/697 (77.76%), Query Frame = 1

Query: 97  RKEEEEEEDNTEEDEEEEGIPSGIGSST---IISSCFVGVLTGIGVVLFNNAVHELRDFF 156
           +++++E+E+  E+++EEE I  G G      ++SS  VG+LTGIGVVLFN AVHE+RD F
Sbjct: 94  QQQDDEDEEEDEDEDEEEMIAQGRGRGNWTIVLSSSLVGLLTGIGVVLFNYAVHEIRDVF 153

Query: 157 WDGIPNRGASWLREMPVEETWKRVILVPACGGFLVSFLNLLREATDNDPS---------- 216
           WDGIP RGASWLRE P+ + WKRVILVPA GG +V+ LN+LR A D+             
Sbjct: 154 WDGIPQRGASWLREEPMGDMWKRVILVPASGGLIVAMLNVLRGALDDGSEDNNNGNEQEE 213

Query: 217 -------TKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGV 276
                   K+G+P+S+ +  +AA +PFLKAVAA VTLGTGNSLGPEGPSV+IGTS+ KGV
Sbjct: 214 EEKESLLVKMGMPISVFHSLKAASRPFLKAVAAGVTLGTGNSLGPEGPSVEIGTSIAKGV 273

Query: 277 STVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADST-FSLTNTTSM 336
             VF ++S+  LSL+AAGSAAGI+SGFNAAV+GCFFAVESVLWPSP+DS+  SLTNTTSM
Sbjct: 274 GAVFGKSSQRSLSLVAAGSAAGIASGFNAAVSGCFFAVESVLWPSPSDSSSISLTNTTSM 333

Query: 337 VILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYML 396
           VILSAVIAS V++VGLG EPAFKVP YDFRSP+ELPLYLLLG LCGLVSL+ SKCTSYML
Sbjct: 334 VILSAVIASVVTEVGLGSEPAFKVPYYDFRSPTELPLYLLLGVLCGLVSLTLSKCTSYML 393

Query: 397 ATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAEL 456
             VD +HK  GV R LFPILGG + GLIALAYPEILYWGFENVD+LLESRP VK LSA+L
Sbjct: 394 VIVDNLHKTIGVPRFLFPILGGLAVGLIALAYPEILYWGFENVDILLESRPLVKGLSADL 453

Query: 457 LAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFE 516
           L QLVVVKI ATSLCRASGLVGGYYAPSLFIGAATGMAYGK I  A+ + N  +  SI E
Sbjct: 454 LLQLVVVKIGATSLCRASGLVGGYYAPSLFIGAATGMAYGKLISSAVVQSNPILHLSILE 513

Query: 517 VASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQ-KR 576
           VASPQAYGLVGMAATL+GVCQVPLT+VLLLFELTQDYRIVLPLLGAVG+SSW+TSGQ +R
Sbjct: 514 VASPQAYGLVGMAATLSGVCQVPLTSVLLLFELTQDYRIVLPLLGAVGLSSWITSGQTRR 573

Query: 577 KRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPNDLCEIESSLCAYDSD 636
           K+ S K+KKL  GK  S QQ      +A+  SS+ A   + +  +DLCEIESSLC  DS+
Sbjct: 574 KKDSWKSKKLNQGKTSSFQQPEVPSPSASGLSSSNAFTERTSNASDLCEIESSLCIDDSN 633

Query: 637 SEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQ 696
            +  +LE++I VSEAM TRYVTV  DT L EAV LMLAEKQ  A+IVD ++ LIG+L L+
Sbjct: 634 IDTEKLEKRIFVSEAMRTRYVTVLKDTLLTEAVTLMLAEKQPCAMIVDNDHILIGLLTLK 693

Query: 697 DIQKLSK----------NGLVVSEVCSLDGEICRVPWTATPSMDILTAKTVMKNLGVSQV 756
           DIQ+ S             L+VSE+CS DGE+C++PWTATPSM++L+A+ +M   G++Q+
Sbjct: 694 DIQEYSNKYAQSRRQRPKELIVSEMCSSDGEVCQIPWTATPSMNLLSAQNIMNRYGMNQI 753

Query: 757 PV----VKDQMGYLVGVLDLECIDLTCRILATRESLG 758
           PV    V+D  G+LVG+LD ECI LTCR LATRESLG
Sbjct: 754 PVVTEHVQDHRGHLVGLLDRECISLTCRALATRESLG 790

BLAST of Cp4.1LG05g13220 vs. TrEMBL
Match: F6I0N4_VITVI (Chloride channel protein OS=Vitis vinifera GN=VIT_03s0038g04260 PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 1.5e-233
Identity = 447/669 (66.82%), Postives = 529/669 (79.07%), Query Frame = 1

Query: 103 EEDNTEEDEEEEGIPSGI--GSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNR 162
           +++ +++D++  G+ S I  G+  I+SSCFVG+LTGIG+VLFNNAVH +RDF WDGIP R
Sbjct: 3   KDEASKDDKKLRGMTSMIEEGNLAILSSCFVGLLTGIGIVLFNNAVHVIRDFSWDGIPYR 62

Query: 163 GASWLREMPVEETWKRVILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAAL 222
           GASWLRE P+E  W+RVILVP CGG LVS LN+LR+A  +     L     ISN  +AAL
Sbjct: 63  GASWLREEPIEAIWERVILVPLCGGLLVSGLNVLRDALQSPGDGNL-----ISN-IKAAL 122

Query: 223 QPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGIS 282
           QPFLKAVAA VTLGTGNSLGPEGPSV+IG S+ KGVS++FD++S+ K+SL+AAGSAAGIS
Sbjct: 123 QPFLKAVAACVTLGTGNSLGPEGPSVEIGASIAKGVSSLFDKSSKRKVSLVAAGSAAGIS 182

Query: 283 SGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVP 342
           SGFNAA AGCFFAVESVLWPS ADS+ SL NTTSMVILSAVIAS VS+VGLG EPAFKVP
Sbjct: 183 SGFNAAFAGCFFAVESVLWPSTADSSLSLQNTTSMVILSAVIASVVSEVGLGSEPAFKVP 242

Query: 343 EYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFST 402
           EYDFRSP ELPLYLLLG LCGLVSL+ SKCTSYML T+D VHK  G+ RA FP+LGG S 
Sbjct: 243 EYDFRSPGELPLYLLLGILCGLVSLALSKCTSYMLVTIDNVHKAVGIPRAAFPVLGGLSV 302

Query: 403 GLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYY 462
           GLIALAYPEILYWGFENVD+LLESRPFVK LSA+LL QLV VKI+ATSLCRASGLVGGYY
Sbjct: 303 GLIALAYPEILYWGFENVDILLESRPFVKGLSADLLLQLVAVKIVATSLCRASGLVGGYY 362

Query: 463 APSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLT 522
           APSLFIGAATGMAYGKFI  A+S+ N     SI EVASPQAYGLVGMAATLAGVCQVPLT
Sbjct: 363 APSLFIGAATGMAYGKFITFAISQSNPMFHLSILEVASPQAYGLVGMAATLAGVCQVPLT 422

Query: 523 AVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDS 582
           AVLLLFELTQDYRIVLPLLGAVG+SSW TS Q +++      KL  G    T  S+ +D 
Sbjct: 423 AVLLLFELTQDYRIVLPLLGAVGLSSWTTSRQAKRKDVGDPGKLKEGNAQKTDLSS-HDP 482

Query: 583 NANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMD 642
           +    SS+Y+   K ++ ++LCE+ESSLC  DS+SE  ELE++I VS+AM TRYVTV M 
Sbjct: 483 SV--LSSSYSVAAKASHASNLCEVESSLCVDDSNSETKELEKRIFVSQAMRTRYVTVLMS 542

Query: 643 TFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSKNG---------LVVSEVCS 702
           T LIEAV L+L EKQ+ A+IVD+++ LIG+L L+DIQ+ S+           ++VSE+CS
Sbjct: 543 TLLIEAVSLLLEEKQTCAVIVDDDHLLIGLLTLEDIQEFSEREKARIRRSKEVLVSEMCS 602

Query: 703 LDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPV----VKDQMGYLVGVLDLECIDLTC 757
           LDGE CRVPWT TP M++ +A+ +M  LGV+Q+PV    V+D  G+ VG+LD ECI L  
Sbjct: 603 LDGEKCRVPWTVTPGMNLFSAQMIMNTLGVNQLPVISDHVEDHKGHPVGLLDRECISLAF 662

BLAST of Cp4.1LG05g13220 vs. TrEMBL
Match: E2GMA7_VITVI (Chloride channel protein OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 5.6e-233
Identity = 457/712 (64.19%), Postives = 545/712 (76.54%), Query Frame = 1

Query: 60  SFCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEEGIPSG 119
           SF  L   G + + + GS   +  R    P A      ++E  +++ +++D++  G+ S 
Sbjct: 24  SFKYLLNYGNNGNKIGGS--WKTGRLVSRPLARNFGGEQKEMGKDEASKDDKKLRGMTSM 83

Query: 120 I--GSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRV 179
           I  G+  I+SSCFVG+LTGIG+VLFNNAVH +RDF WDGIP RGASWLRE P+E  W+RV
Sbjct: 84  IEEGNLAILSSCFVGLLTGIGIVLFNNAVHVIRDFSWDGIPYRGASWLREEPIEAIWERV 143

Query: 180 ILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGN 239
           ILVP CGG LVS LN+LR+A  +     L     ISN  +AALQPFLKAVAA VTLGTGN
Sbjct: 144 ILVPLCGGLLVSGLNVLRDALQSPGDGNL-----ISN-IKAALQPFLKAVAACVTLGTGN 203

Query: 240 SLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESV 299
           SLGPEGPSV+IG S+ KGVS++FD++S+ K+SL+AAGSAAGISSGFNAA AGCFFAVESV
Sbjct: 204 SLGPEGPSVEIGASIAKGVSSLFDKSSKRKVSLVAAGSAAGISSGFNAAFAGCFFAVESV 263

Query: 300 LWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLG 359
           LWPS ADS+ SL NTTSMVILSAVIAS VS+VGLG EPAFKVPEYDFRSP ELPLYLLLG
Sbjct: 264 LWPSTADSSLSLQNTTSMVILSAVIASVVSEVGLGSEPAFKVPEYDFRSPGELPLYLLLG 323

Query: 360 ALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFEN 419
            LCGLVSL+ SKCTSYML T+D VHK  G+ RA FP+LGG S GLIALAYPEILYWGFEN
Sbjct: 324 ILCGLVSLALSKCTSYMLVTIDNVHKAVGIPRAAFPVLGGLSVGLIALAYPEILYWGFEN 383

Query: 420 VDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKF 479
           VD+LLESRPFVK LSA+LL QLV VKI+ATSLCRASGLVGGYYAPSLFIGAATGMAYGKF
Sbjct: 384 VDILLESRPFVKGLSADLLLQLVAVKIVATSLCRASGLVGGYYAPSLFIGAATGMAYGKF 443

Query: 480 IGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLP 539
           I  A+S+ N     SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLP
Sbjct: 444 ITFAISQSNPMFHLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLP 503

Query: 540 LLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETY 599
           LLGAVG+SSW TS Q +++      KL  G    T  S+ +D +    SS+Y+   K ++
Sbjct: 504 LLGAVGLSSWTTSRQAKRKDVGDPGKLKEGNAQKTDLSS-HDPSV--LSSSYSVAAKASH 563

Query: 600 PNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSY 659
            ++LCE+ESSLC  DS+SE  ELE++I VS+AM TRYVTV M T LIEAV L+L EKQ+ 
Sbjct: 564 ASNLCEVESSLCVDDSNSETKELEKRIFVSQAMRTRYVTVLMSTLLIEAVSLLLEEKQTC 623

Query: 660 ALIVDEENTLIGILALQDIQKLSKNG---------LVVSEVCSLDGEICRVPWTATPSMD 719
           A+IVD+++ LIG+L L+DIQ+ S+           ++VSE+CSLDGE CRVPWT TP M+
Sbjct: 624 AVIVDDDHLLIGLLTLEDIQEFSEREKARIRRSKEVLVSEMCSLDGEKCRVPWTVTPGMN 683

Query: 720 ILTAKTVMKNLGVSQVPV----VKDQMGYLVGVLDLECIDLTCRILATRESL 757
           + +A+ +M  LGV+Q+PV    V+D  G+ VG+LD ECI L  R +A RE L
Sbjct: 684 LFSAQMIMNTLGVNQLPVISDHVEDHKGHPVGLLDRECISLAFRAVAAREYL 724

BLAST of Cp4.1LG05g13220 vs. TrEMBL
Match: A0A061E9K1_THECC (Chloride channel protein OS=Theobroma cacao GN=TCM_007622 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 5.4e-228
Identity = 434/649 (66.87%), Postives = 500/649 (77.04%), Query Frame = 1

Query: 122 SSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILVP 181
           S  IIS+C VGV TG+GVVLFNN VHE+RDFFWDGIP RGASWLRE P++  W RVILVP
Sbjct: 87  SGVIISACLVGVFTGLGVVLFNNGVHEIRDFFWDGIPYRGASWLREEPLDSVWTRVILVP 146

Query: 182 ACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLGP 241
           ACGG +VS LN  R+A               S   + AL  FLK VAA VTLG+GNSLGP
Sbjct: 147 ACGGLIVSVLNAARDAVSK-----------ASYSAKTALGSFLKTVAACVTLGSGNSLGP 206

Query: 242 EGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWPS 301
           EGPSV+IG+S+ K + ++ D+N +TKLSL+AAGSAAGISSGFNAAVAGCFFAVESVLWPS
Sbjct: 207 EGPSVEIGSSIAKEIHSILDKNPQTKLSLLAAGSAAGISSGFNAAVAGCFFAVESVLWPS 266

Query: 302 -PADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALC 361
            PADS+ SLTNTTSMVILSAVIAS VS+VGLG EP+FKVPEYDFRS  ELPLYLLLG LC
Sbjct: 267 SPADSSVSLTNTTSMVILSAVIASVVSEVGLGSEPSFKVPEYDFRSAGELPLYLLLGILC 326

Query: 362 GLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVDL 421
           GLVSL+FSK TSY+L  VD ++KD G+ + +FP++GG + G+IALAYPEILYWGFENVD+
Sbjct: 327 GLVSLTFSKLTSYLLGVVDNLNKDVGIPKPVFPVVGGLTVGVIALAYPEILYWGFENVDI 386

Query: 422 LLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGI 481
           LLESRPFVK LS +LL QLV VKI+ATS+CRASGLVGGYYAPSLFIGAATGMAYGKFI  
Sbjct: 387 LLESRPFVKGLSGDLLFQLVAVKIIATSVCRASGLVGGYYAPSLFIGAATGMAYGKFISF 446

Query: 482 ALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLG 541
           A+++ N AI  SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLG
Sbjct: 447 AIAQSNPAIHLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLG 506

Query: 542 AVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPND 601
           AVG SSW+TSG+ R++  Q  K L  G   ++QQ    DS A   SS  A    E Y  +
Sbjct: 507 AVGFSSWITSGRMRRKDVQGKKALKDGNTRTSQQPKASDS-ATGLSSMDAPPETEPYLRN 566

Query: 602 LCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALI 661
           LCE+ESSLC  DS     ELE++I VSEAM TRYVTV M T L +AV LML EKQS A+I
Sbjct: 567 LCEVESSLCIDDSSIRTKELEKRIFVSEAMRTRYVTVMMSTLLTQAVTLMLVEKQSCAII 626

Query: 662 VDEENTLIGILALQDIQKLSK---------NGLVVSEVCSLDGEICRVPWTATPSMDILT 721
           VD++N L+G+L L DI +  K           L+VSE+CSLD   C+VPWTATP+MD+L+
Sbjct: 627 VDDDNLLMGLLTLTDIHEFGKFVKDKCLESKELLVSEICSLDSAKCKVPWTATPTMDLLS 686

Query: 722 AKTVMKNLGVSQVPV----VKDQMGYLVGVLDLECIDLTCRILATRESL 757
           A+ +M   G+SQVPV    VKD  G  VG+LD ECI LTCR LATRESL
Sbjct: 687 AEIIMTKHGLSQVPVISEHVKDCRGQPVGLLDRECISLTCRALATRESL 723

BLAST of Cp4.1LG05g13220 vs. TAIR10
Match: AT4G35440.2 (AT4G35440.2 chloride channel E)

HSP 1 Score: 728.0 bits (1878), Expect = 5.9e-210
Identity = 405/656 (61.74%), Postives = 486/656 (74.09%), Query Frame = 1

Query: 108 EEDEEEEGIPSGIGSS-TIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLR 167
           E D++E G          I S+C VGVLTG+ VVLFNN VH LRDF WDGIP+RGASWLR
Sbjct: 59  ETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRGASWLR 118

Query: 168 EMPVEETWKRVILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKA 227
           E P+   W RVILVP  GG +VS LN LRE+         G   S  ++ +A L+PFLK 
Sbjct: 119 EAPIGSNWLRVILVPTIGGLVVSILNQLRESAGKST----GDSHSSLDRVKAVLRPFLKT 178

Query: 228 VAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAA 287
           VAA VTLGTGNSLGPEGPSV+IG S+ KGV+++F+++ +T  SL+AAGSAAGISSGFNAA
Sbjct: 179 VAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLFNKSPQTGFSLLAAGSAAGISSGFNAA 238

Query: 288 VAGCFFAVESVLWPSPA-DSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFR 347
           VAGCFFAVESVLWPS + DS+ SL NTTSMVILSAV AS VS++GLG EPAFKVP+YDFR
Sbjct: 239 VAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFR 298

Query: 348 SPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIAL 407
           SP ELPLYLLLGALCGLVSL+ S+CTS M + VD ++KD G+ +A+FP++GG S G+IAL
Sbjct: 299 SPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIAL 358

Query: 408 AYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLF 467
            YPE+LYWGF+NVD+LLE RPFVK LSA+LL QLV VKI AT+ CRASGLVGGYYAPSLF
Sbjct: 359 VYPEVLYWGFQNVDILLEKRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLF 418

Query: 468 IGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 527
           IG A GMAYGKFIG+AL++ N   + SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLL
Sbjct: 419 IGGAAGMAYGKFIGLALAQ-NPDFNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 478

Query: 528 FELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQ 587
           FELTQDYRIVLPLLGAVG+SSW+TSGQ +++ +++TK+    K     QS T   +++D+
Sbjct: 479 FELTQDYRIVLPLLGAVGMSSWITSGQSKRQETRETKETRKRKSQEAVQSLT---SSDDE 538

Query: 588 SSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIE 647
           SS           N+LCE+ESSLC  DS ++  EL + I VSEAM TR+ TV M T L E
Sbjct: 539 SST----------NNLCEVESSLCLDDSLNQSEELPKSIFVSEAMRTRFATVMMSTSLEE 598

Query: 648 AVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSK---------NGLVVSEVCSLDGEI 707
           A+  ML EKQS ALIVD +N  +GIL L DIQ+ SK           + V+++CS  G  
Sbjct: 599 ALTRMLIEKQSCALIVDPDNIFLGILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSGGK 658

Query: 708 CRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMG----YLVGVLDLECIDLTCR 749
           C+VPWT TP MD+L A+T+M    +S V VV   +     + VGVLD ECI LT R
Sbjct: 659 CKVPWTVTPDMDLLAAQTIMNKHELSHVAVVSGSIDAPRIHPVGVLDRECITLTRR 696

BLAST of Cp4.1LG05g13220 vs. TAIR10
Match: AT1G55620.2 (AT1G55620.2 chloride channel F)

HSP 1 Score: 410.6 bits (1054), Expect = 2.1e-114
Identity = 281/700 (40.14%), Postives = 398/700 (56.86%), Query Frame = 1

Query: 46  RFSLRPKQTGFRFRSFCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEED 105
           RFSL  ++  F+     ++    E  +P   SS   FS   G     GI      ++  D
Sbjct: 59  RFSLSGRRLSFKRME--NIRVDRERHNP---SSSSAFSAA-GEEDGGGISNLHSVDDRND 118

Query: 106 NTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWL 165
               DEE  G  +    + ++  C +GV  GI V  FN  VH + ++ W G PN GA+WL
Sbjct: 119 EYGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAWL 178

Query: 166 REMPVEETWKRVILVPACGGFLVSFLNLLREATD----NDPSTKLGVPVSISNQFRAALQ 225
           R   + +TW R++L+P  GG +V  ++ L E  D    ++ S + G+       F A + 
Sbjct: 179 RLQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGL------DFLAGIY 238

Query: 226 PFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISS 285
           P +KA+ A+VTLGTG SLGPEGPSVDIG S   G + + + N   +++L AAG+A+GI+S
Sbjct: 239 PVIKAIQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRERRIALTAAGAASGIAS 298

Query: 286 GFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPE 345
           GFNAAVAGCFFA+E+VL P  A++  S   TT+M+IL++VI+S VS   LG + AF VP 
Sbjct: 299 GFNAAVAGCFFAIETVLRPLRAEN--SPPFTTAMIILASVISSTVSNALLGTQSAFTVPS 358

Query: 346 YDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTG 405
           YD +S +ELPLYL+LG LCG VS+ FS+  ++   + D +   FG+   + P LGG   G
Sbjct: 359 YDLKSAAELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAG 418

Query: 406 LIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYA 465
           +IAL YP ILYWGF NV+ +L +          LLAQL   K++AT+LC+ SGLVGG YA
Sbjct: 419 IIALKYPGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYA 478

Query: 466 PSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTA 525
           PSL IGAA G  +G   G A    N AI  +   VA PQAY LVGMAATLA +C VPLT+
Sbjct: 479 PSLMIGAAVGAVFG---GSAAEIINRAIPGNA-AVAQPQAYALVGMAATLASMCSVPLTS 538

Query: 526 VLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSN 585
           VLLLFELT+DYRI+LPL+GAVG++ W+ S   + + S  ++    G+  S+   +   + 
Sbjct: 539 VLLLFELTKDYRILLPLMGAVGLAIWVPSVANQGKESDSSEGRSTGRGYSSLSPSERKTE 598

Query: 586 ANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDT 645
              + ++ AD  + T    + E        D ++ + +L+    V   M+  YV V   T
Sbjct: 599 GVWRHTDNADSLELT----VIENPDHNSFLDEETILEDLK----VMRVMSKNYVKVSSGT 658

Query: 646 FLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQK---------LSKNGLVVSEVC-- 705
            L EA +++    Q+  ++VD+++ L GIL   DI++         L +N   VS VC  
Sbjct: 659 TLREARNILKESHQNCIMVVDDDDFLAGILTHGDIRRYLSNNASTILDENTCPVSSVCTK 718

Query: 706 --SLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVK 729
             S  G+  R   T  P   +  AK +M+  GV Q+PVVK
Sbjct: 719 KISYRGQE-RGLLTCYPDATVGVAKELMEARGVKQLPVVK 731

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: gi|778712463|ref|XP_011656898.1| (PREDICTED: chloride channel protein CLC-e isoform X1 [Cucumis sativus])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 625/806 (77.54%), Postives = 681/806 (84.49%), Query Frame = 1

Query: 1   MTVYTMGAIDSIGIRLNNNAHHYP------------------LSSSISAPNDC------- 60
           M +  MGA DS+G++LNN A HYP                   SSSIS  +D        
Sbjct: 1   MIISIMGAFDSMGLKLNN-APHYPCLSSLPSAFFCSNFSTLNFSSSISGLHDLSIPISNG 60

Query: 61  ---CS----SYGRFLGLRFSLRPKQTGFRFRSFCSLPGSGESESPVPGSSDGRFSRGEGS 120
              C+    SY   LGL FSLRPK+T   FR   +LPGSGESESP+  SS+  FS  +  
Sbjct: 61  LENCAVGNRSYDSLLGLHFSLRPKRTASCFRPISALPGSGESESPISVSSNAGFSIKQSE 120

Query: 121 PSATGIKWRKEEEEEEDNTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHE 180
                 ++  ++ EEE+  EE+EEEEGIP G GSSTIISSCFVG+LTGIGVVLFNNAVHE
Sbjct: 121 QE----EYDDDDAEEEEEEEEEEEEEGIPFGTGSSTIISSCFVGLLTGIGVVLFNNAVHE 180

Query: 181 LRDFFWDGIPNRGASWLREMPVEETWKRVILVPACGGFLVSFLNLLREATD--------N 240
           +RDF WDGIPNRGASWLREMP+E+ WKRVILVPA GGFLVSFLNLLR+ATD        +
Sbjct: 181 IRDFSWDGIPNRGASWLREMPIEDIWKRVILVPASGGFLVSFLNLLRDATDVKVDQPQGD 240

Query: 241 DPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVF 300
           DPSTK GVP+SISN+ +AALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKG+STVF
Sbjct: 241 DPSTKFGVPISISNKLKAALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVF 300

Query: 301 DRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSA 360
           + NSRTKLSLIAAGSAAGISSGFNAAVAGCFFA+ESVLWPSPADSTFSLTNTTSMVILSA
Sbjct: 301 EMNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAIESVLWPSPADSTFSLTNTTSMVILSA 360

Query: 361 VIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDK 420
           VIAS VSQVGLGVEPAFKVP YDFRSPSELPLYLLLG LCGLVSLSFSKCTSY+LATVDK
Sbjct: 361 VIASVVSQVGLGVEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSKCTSYLLATVDK 420

Query: 421 VHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLV 480
            HK+FG  RA+FPILGGF+ GLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLV
Sbjct: 421 FHKEFGAPRAMFPILGGFTIGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLV 480

Query: 481 VVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQ 540
           V+KILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPN+ +DFSIFEVASPQ
Sbjct: 481 VIKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQ 540

Query: 541 AYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQK 600
           AYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSW+TSGQKRKRSSQ+
Sbjct: 541 AYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQQ 600

Query: 601 TKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVEL 660
           TKKL  GK  STQQST YDSNAN+QSSNYA+DG+  YPNDLCEIESSLCAY+SDSE VEL
Sbjct: 601 TKKLSPGKSPSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSLCAYESDSETVEL 660

Query: 661 ERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLS 720
           ERKI VSEAMTT+Y+T+ M T L+EAV+LMLAEKQS ALIVDE N LIGIL L+DIQKLS
Sbjct: 661 ERKISVSEAMTTKYITILMGTCLVEAVNLMLAEKQSCALIVDEGNILIGILELEDIQKLS 720

Query: 721 KNG---------LVVSEVCSLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQM 758
           KN           VVSE+CSLD ++CRVPWTATPSMDILTAK +MKNLGV+QVPVV+DQM
Sbjct: 721 KNAKSRNEQLKDFVVSEICSLDEKMCRVPWTATPSMDILTAKMIMKNLGVTQVPVVRDQM 780

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: gi|659094609|ref|XP_008448151.1| (PREDICTED: chloride channel protein CLC-e isoform X1 [Cucumis melo])

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 621/806 (77.05%), Postives = 681/806 (84.49%), Query Frame = 1

Query: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSS-----------------SISAPNDCCS------ 60
           M +  +GA DS+G++LNN  ++  LSS                 SISA +D  +      
Sbjct: 1   MIISIIGAFDSMGLKLNNAPNYLGLSSLPSASFSSNFSTLTFSSSISALHDLSNPISNGL 60

Query: 61  --------SYGRFLGLRFSLRPKQTGFRFRSFCSLPGSGESESPVPGSSDGRFSRGEGSP 120
                   SY   LGL FSLRPK+T   FRS  +LPGS ESESP+  SS+  FS      
Sbjct: 61  KNCAVGNRSYDSLLGLHFSLRPKRTASYFRSISALPGSEESESPISVSSNAAFS------ 120

Query: 121 SATGIKWRKEEEEEEDNTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHEL 180
               IK  +EEEE++D+  E+EEEEGIP GIGSSTIISSCFVG+LTGIGVVLFNNAVHE+
Sbjct: 121 ----IKQSEEEEEDDDDDAEEEEEEGIPYGIGSSTIISSCFVGLLTGIGVVLFNNAVHEI 180

Query: 181 RDFFWDGIPNRGASWLREMPVEETWKRVILVPACGGFLVSFLNLLREATD---------N 240
           R+F WDGIPNRGASWLREMP+E+ WKRVILVPA GG LVSFLNLLR+ATD         +
Sbjct: 181 RNFSWDGIPNRGASWLREMPIEDIWKRVILVPASGGALVSFLNLLRDATDVKVDQPQEGD 240

Query: 241 DPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVF 300
           DPSTK GVP+SISN+ + ALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKG+STVF
Sbjct: 241 DPSTKFGVPISISNKLKVALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVF 300

Query: 301 DRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSA 360
           ++NSRTKLSLIAAGSAAGISSGFNAAVAGCFFA+ESVLWPSPADSTFSLTNTTSMVILSA
Sbjct: 301 EKNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAIESVLWPSPADSTFSLTNTTSMVILSA 360

Query: 361 VIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDK 420
           VIAS VSQVGLG EPAFKVP YDFRSPSELPLYLLLG LCGLVSLSFSKCTSY+LATVDK
Sbjct: 361 VIASVVSQVGLGAEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSKCTSYLLATVDK 420

Query: 421 VHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLV 480
            HKDFG  RA+FPILGGF+TGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLV
Sbjct: 421 FHKDFGAPRAMFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLV 480

Query: 481 VVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQ 540
           V+KILATSLCRA GLVGGYYAPSLFIGAATGMAYGKFIG+ALSEPN+ +DFSIFEVASPQ
Sbjct: 481 VIKILATSLCRACGLVGGYYAPSLFIGAATGMAYGKFIGLALSEPNSVLDFSIFEVASPQ 540

Query: 541 AYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQK 600
           AYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSW+TSGQKRKRSSQ+
Sbjct: 541 AYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQQ 600

Query: 601 TKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVEL 660
           TKKL  GK  STQQST YDSNAN+QSSNYA+DG+  YPNDLCEIESSLCAY+SDSE VEL
Sbjct: 601 TKKLSSGKSPSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSLCAYESDSETVEL 660

Query: 661 ERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLS 720
           ERKI VSEAMTT+Y+T+ M T L+EAV+LMLAEKQS  LIVDE N LIGIL L++IQKLS
Sbjct: 661 ERKISVSEAMTTKYITILMGTCLVEAVNLMLAEKQSCVLIVDEGNILIGILELENIQKLS 720

Query: 721 KNG---------LVVSEVCSLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQM 758
           KN          +VVSE+CSLDG++CRVPWTATPSMDILTAK +MKNLGV+QVPVV+DQM
Sbjct: 721 KNAKSRGEQLKDVVVSEICSLDGKMCRVPWTATPSMDILTAKMIMKNLGVTQVPVVRDQM 780

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: gi|700191429|gb|KGN46633.1| (hypothetical protein Csa_6G116660 [Cucumis sativus])

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 579/677 (85.52%), Postives = 621/677 (91.73%), Query Frame = 1

Query: 98  KEEEEEEDNTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGI 157
           +EEEEEE+  EE+EEEEGIP G GSSTIISSCFVG+LTGIGVVLFNNAVHE+RDF WDGI
Sbjct: 276 EEEEEEEEEEEEEEEEEGIPFGTGSSTIISSCFVGLLTGIGVVLFNNAVHEIRDFSWDGI 335

Query: 158 PNRGASWLREMPVEETWKRVILVPACGGFLVSFLNLLREATD--------NDPSTKLGVP 217
           PNRGASWLREMP+E+ WKRVILVPA GGFLVSFLNLLR+ATD        +DPSTK GVP
Sbjct: 336 PNRGASWLREMPIEDIWKRVILVPASGGFLVSFLNLLRDATDVKVDQPQGDDPSTKFGVP 395

Query: 218 VSISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLS 277
           +SISN+ +AALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKG+STVF+ NSRTKLS
Sbjct: 396 ISISNKLKAALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFEMNSRTKLS 455

Query: 278 LIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQV 337
           LIAAGSAAGISSGFNAAVAGCFFA+ESVLWPSPADSTFSLTNTTSMVILSAVIAS VSQV
Sbjct: 456 LIAAGSAAGISSGFNAAVAGCFFAIESVLWPSPADSTFSLTNTTSMVILSAVIASVVSQV 515

Query: 338 GLGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSR 397
           GLGVEPAFKVP YDFRSPSELPLYLLLG LCGLVSLSFSKCTSY+LATVDK HK+FG  R
Sbjct: 516 GLGVEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSKCTSYLLATVDKFHKEFGAPR 575

Query: 398 ALFPILGGFSTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSL 457
           A+FPILGGF+ GLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVV+KILATSL
Sbjct: 576 AMFPILGGFTIGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVIKILATSL 635

Query: 458 CRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAA 517
           CRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPN+ +DFSIFEVASPQAYGLVGMAA
Sbjct: 636 CRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGMAA 695

Query: 518 TLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKV 577
           TLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSW+TSGQKRKRSSQ+TKKL  GK 
Sbjct: 696 TLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQQTKKLSPGKS 755

Query: 578 LSTQQSTTYDSNANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEA 637
            STQQST YDSNAN+QSSNYA+DG+  YPNDLCEIESSLCAY+SDSE VELERKI VSEA
Sbjct: 756 PSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSLCAYESDSETVELERKISVSEA 815

Query: 638 MTTRYVTVFMDTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSKNG------ 697
           MTT+Y+T+ M T L+EAV+LMLAEKQS ALIVDE N LIGIL L+DIQKLSKN       
Sbjct: 816 MTTKYITILMGTCLVEAVNLMLAEKQSCALIVDEGNILIGILELEDIQKLSKNAKSRNEQ 875

Query: 698 ---LVVSEVCSLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDL 757
               VVSE+CSLD ++CRVPWTATPSMDILTAK +MKNLGV+QVPVV+DQMGY+VGVLD 
Sbjct: 876 LKDFVVSEICSLDEKMCRVPWTATPSMDILTAKMIMKNLGVTQVPVVRDQMGYVVGVLDW 935

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: gi|778712475|ref|XP_011656899.1| (PREDICTED: chloride channel protein CLC-e isoform X2 [Cucumis sativus])

HSP 1 Score: 988.4 bits (2554), Expect = 6.9e-285
Identity = 519/607 (85.50%), Postives = 556/607 (91.60%), Query Frame = 1

Query: 168 MPVEETWKRVILVPACGGFLVSFLNLLREATD--------NDPSTKLGVPVSISNQFRAA 227
           MP+E+ WKRVILVPA GGFLVSFLNLLR+ATD        +DPSTK GVP+SISN+ +AA
Sbjct: 1   MPIEDIWKRVILVPASGGFLVSFLNLLRDATDVKVDQPQGDDPSTKFGVPISISNKLKAA 60

Query: 228 LQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGI 287
           LQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKG+STVF+ NSRTKLSLIAAGSAAGI
Sbjct: 61  LQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFEMNSRTKLSLIAAGSAAGI 120

Query: 288 SSGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKV 347
           SSGFNAAVAGCFFA+ESVLWPSPADSTFSLTNTTSMVILSAVIAS VSQVGLGVEPAFKV
Sbjct: 121 SSGFNAAVAGCFFAIESVLWPSPADSTFSLTNTTSMVILSAVIASVVSQVGLGVEPAFKV 180

Query: 348 PEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFS 407
           P YDFRSPSELPLYLLLG LCGLVSLSFSKCTSY+LATVDK HK+FG  RA+FPILGGF+
Sbjct: 181 PVYDFRSPSELPLYLLLGVLCGLVSLSFSKCTSYLLATVDKFHKEFGAPRAMFPILGGFT 240

Query: 408 TGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGY 467
            GLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVV+KILATSLCRASGLVGGY
Sbjct: 241 IGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVIKILATSLCRASGLVGGY 300

Query: 468 YAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPL 527
           YAPSLFIGAATGMAYGKFIGIALSEPN+ +DFSIFEVASPQAYGLVGMAATLAGVCQVPL
Sbjct: 301 YAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGMAATLAGVCQVPL 360

Query: 528 TAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYD 587
           TAVLLLFELTQDYRIVLPLLGAVGVSSW+TSGQKRKRSSQ+TKKL  GK  STQQST YD
Sbjct: 361 TAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQQTKKLSPGKSPSTQQSTAYD 420

Query: 588 SNANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFM 647
           SNAN+QSSNYA+DG+  YPNDLCEIESSLCAY+SDSE VELERKI VSEAMTT+Y+T+ M
Sbjct: 421 SNANNQSSNYAEDGQTNYPNDLCEIESSLCAYESDSETVELERKISVSEAMTTKYITILM 480

Query: 648 DTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSKNG---------LVVSEVC 707
            T L+EAV+LMLAEKQS ALIVDE N LIGIL L+DIQKLSKN           VVSE+C
Sbjct: 481 GTCLVEAVNLMLAEKQSCALIVDEGNILIGILELEDIQKLSKNAKSRNEQLKDFVVSEIC 540

Query: 708 SLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRIL 758
           SLD ++CRVPWTATPSMDILTAK +MKNLGV+QVPVV+DQMGY+VGVLD ECIDLTCRIL
Sbjct: 541 SLDEKMCRVPWTATPSMDILTAKMIMKNLGVTQVPVVRDQMGYVVGVLDWECIDLTCRIL 600

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: gi|659094611|ref|XP_008448152.1| (PREDICTED: chloride channel protein CLC-e isoform X2 [Cucumis melo])

HSP 1 Score: 985.7 bits (2547), Expect = 4.5e-284
Identity = 516/608 (84.87%), Postives = 555/608 (91.28%), Query Frame = 1

Query: 168 MPVEETWKRVILVPACGGFLVSFLNLLREATD---------NDPSTKLGVPVSISNQFRA 227
           MP+E+ WKRVILVPA GG LVSFLNLLR+ATD         +DPSTK GVP+SISN+ + 
Sbjct: 1   MPIEDIWKRVILVPASGGALVSFLNLLRDATDVKVDQPQEGDDPSTKFGVPISISNKLKV 60

Query: 228 ALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAG 287
           ALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKG+STVF++NSRTKLSLIAAGSAAG
Sbjct: 61  ALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFEKNSRTKLSLIAAGSAAG 120

Query: 288 ISSGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFK 347
           ISSGFNAAVAGCFFA+ESVLWPSPADSTFSLTNTTSMVILSAVIAS VSQVGLG EPAFK
Sbjct: 121 ISSGFNAAVAGCFFAIESVLWPSPADSTFSLTNTTSMVILSAVIASVVSQVGLGAEPAFK 180

Query: 348 VPEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGF 407
           VP YDFRSPSELPLYLLLG LCGLVSLSFSKCTSY+LATVDK HKDFG  RA+FPILGGF
Sbjct: 181 VPVYDFRSPSELPLYLLLGVLCGLVSLSFSKCTSYLLATVDKFHKDFGAPRAMFPILGGF 240

Query: 408 STGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGG 467
           +TGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVV+KILATSLCRA GLVGG
Sbjct: 241 TTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVIKILATSLCRACGLVGG 300

Query: 468 YYAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVP 527
           YYAPSLFIGAATGMAYGKFIG+ALSEPN+ +DFSIFEVASPQAYGLVGMAATLAGVCQVP
Sbjct: 301 YYAPSLFIGAATGMAYGKFIGLALSEPNSVLDFSIFEVASPQAYGLVGMAATLAGVCQVP 360

Query: 528 LTAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTY 587
           LTAVLLLFELTQDYRIVLPLLGAVGVSSW+TSGQKRKRSSQ+TKKL  GK  STQQST Y
Sbjct: 361 LTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQQTKKLSSGKSPSTQQSTAY 420

Query: 588 DSNANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVF 647
           DSNAN+QSSNYA+DG+  YPNDLCEIESSLCAY+SDSE VELERKI VSEAMTT+Y+T+ 
Sbjct: 421 DSNANNQSSNYAEDGQTNYPNDLCEIESSLCAYESDSETVELERKISVSEAMTTKYITIL 480

Query: 648 MDTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSKNG---------LVVSEV 707
           M T L+EAV+LMLAEKQS  LIVDE N LIGIL L++IQKLSKN          +VVSE+
Sbjct: 481 MGTCLVEAVNLMLAEKQSCVLIVDEGNILIGILELENIQKLSKNAKSRGEQLKDVVVSEI 540

Query: 708 CSLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRI 758
           CSLDG++CRVPWTATPSMDILTAK +MKNLGV+QVPVV+DQMGYLVGVLD ECIDLTCRI
Sbjct: 541 CSLDGKMCRVPWTATPSMDILTAKMIMKNLGVTQVPVVRDQMGYLVGVLDWECIDLTCRI 600