Cp4.1LG05g12550 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG05g12550
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG05 : 8824096 .. 8826852 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCTGTAGATATGAGCACTGCCAGGCTGATCAGGGTAGACCAATCTGGAAAGGGAGATTTTAGGAAGATTCAAGAAGCCATTGATTCTGTTCCATCCCGAAACAATGAACTCGTTTTCATTTGGATCAAACCCGGAACTTATAGGTTTGTTTTTCGAATTATACGCTTTAATTCGTTGAGTTATGCTCGAGTTTAGACCATGCTTATAATCCTCGTTCTTTAACCTCTTTTAGCTCGGTATTCTTTCATGTTTCATGCATCGATTTTTGTATTTTCGTGTTTTTGTTAGTTTATATCGAGAGAAAACTTTCGTTAAATTAGATTATGTTTAGGTTGGTAGCTCTTAGGTTAGTTAGGATTATTATTAGTCTTGGTCATTTTGGATANTATTAAAAAAATAAAAATTTGAAATAAATAATTTTTTGAATTTATTATTTAATAAAAATTAGTAATTTAAAAAAATTATTGTCGGAAACTAACGATCTAATTCAACCGAGCCGGTATAGTTCAATTGATAGAGCAGTGGACTGAAAATCCTATTGAAAATAAGAATAATACTTCATTAAAAGAAAAATAATTATATTGGAACCAAAATTATATATATGAAAAATCGCAAAATTATTATTTTAAAAAGACACGTAACACTATTTTTTCATGGGTTTTATTAAATCGTCACGTCATTAATTTATTTAAAAATTGTAAATTTATAACTTTATAGTAAAGATTGAATTAAAAATAATACTATTAAAAGAAAATAAATATTCAAACTTCAAAATGAAATAACATTTTTGGAATTGAAGGAAAATCCCCACGTGGGCACATGGGCCTTTTGCCATTAAACCAAGCTACAAATTATATATATATATATATATATATATATATATATATATATATATATATATATATATGAGGGGAAATTTGAAATTGAATTAATTTGGTGATTTTAATAAGCTTTATTTTGAAATTTCTTTGAATAATAGCGATGGCTCCTGTAGATATGAGCACTGCCAGGCTGATCAGGGTAGACCAATCTGGAAAGGGAGATTTTAGGAAGATTCAAGAAGCCATTGATTCTGTTCCATCCCGAAACAATGAACTCGTTTTCATTTGGATCAAACCCGGAACTTATAGGTTTGTTTTTCGAATTATACGCTTTAATTCGTTGAGTTATGCTCGAGTTTAGACCATGCTTATAATCCTCGTTCTTTAACCTCTTTTAGCTCGGTATTCTTTCATGTTTCATGCATCGATTTTTGTATTTTCGTGTTTTTGTTAGTTTATATCGAGAGAAAACTTTCGTTAAATTAGATTATGTTTAGGTTGGTAGCTCTTAGGTTAGTTAGGATTATTATTAGTCTTGGTCATTTTGGATAAGTCGAAATAAGATGATGACCGTTCATAATTCATCATCACGTTGCCCACTTCCAGTTGATGACGGGCTATTTAATACGTGATGACCTGAGTCCCTCTAGAGCTTGACTTGGGAACCATATGTGAGTCAAGCTGCTTCGTATGACTCTTCCTAAGAACTTAAAAGTCTCGGAATGCTGAAATTTTTAATAGGAGTCTTCACATGATTCGAGAACCCGATGAACCCAGACTACCTAGCCCAAACCATAAAAGTTGAGTCGGGTTTATTTTTTGAAAATTCGAAAATCTGGGTCGGTTCAAGTTGCTATTTTTTTTGTCGGGTCAATTCGACCGACTCATATTTTATTTTATTTTTTTGAGCAGGGAGAAGATTGTGGTGCCGGAGGACAAGCCGTTCATAACATTGAGCGGCAGCAAAGCCTCCGACACCATAATCACATGGAACCAAGGAAGAGATTTACTTCAATCTCCGGCCATCTCTATCTTCGCCGCGGACTTCGTCGGCCGTTTTCTGACCATCCAGAACACATACGGCACCACCGGCATTGCGGTGGCGCTGAGGGTCTCCGCCGACAGAGCCGCCTTCTACGGCTGCCGGATCATATCCTTTCAAGACACCCTTCTTGACGACATCGGAAAACACTACTTCAAGAACTGTTACATCGAAGGCGCCACCGATTTCATCTGCGGAAACGCCGCTTCTCTCTACGAGGTTTTTTTTTTTTTTTGAGAGATACATAGACACTCTTGCTAAAACCAATTGAGTAATAAATAGTAAGAGTAATAAATGGATACAATATATTTGTCTCTAATAATAAATGTCTGTAACAATATATTTGTTTGCAGAAGTGTCATTTGCACTCAACGTCGGAGAGAGGCGGGGCGATGACGGCACAGCACCGTAACACAGTGGCGGAGAACACTGGGTTCGTGTTCTTGGGGTGCAAAATTACAGGAAGTGGATCGACTTTTCTTGGACGGCCATGGGGGGATTGCTCAAGAGTTGTGTTCGGCTACACTCATATGAGCAACGTTATTGTGCCGGAGGGCTGGAATGACTGGGGAGATCACACCAAGCAAAGGTAATACTTTTTCGATCCTAAAATACTATCTCTGCGAAGTTTTGTGATTTCTATCCTTTTTTTCGTATGAGATTTTGAATCTAAAGATGAATTTGTGTTGCATAACATATAGAACGGTGTTCTACGGGGAATACAAATGCTACGGACCAGGGGCGAACAGAGCAAAAAGAGTAGCGTGGTCGCGCAGCCTCTCGGCGGATGAGGCTGCAAAACTCTTCACGAAAGACATGATCGGCGGTCGAGGTTGGCTCCGACCTGCTCCGTCTCATTTCAAGAGGAGCTCCTCCACCATTGTCTAG

mRNA sequence

ATGGCTCCTGTAGATATGAGCACTGCCAGGCTGATCAGGGTAGACCAATCTGGAAAGGGAGATTTTAGGAAGATTCAAGAAGCCATTGATTCTGTTCCATCCCGAAACAATGAACTCGTTTTCATTTGGATCAAACCCGGAACTTATAGGCTGATCAGGGTAGACCAATCTGGAAAGGGAGATTTTAGGAAGATTCAAGAAGCCATTGATTCTGTTCCATCCCGAAACAATGAACTCGTTTTCATTTGGATCAAACCCGGAACTTATAGCAAAGCCTCCGACACCATAATCACATGGAACCAAGGAAGAGATTTACTTCAATCTCCGGCCATCTCTATCTTCGCCGCGGACTTCGTCGGCCGTTTTCTGACCATCCAGAACACATACGGCACCACCGGCATTGCGGTGGCGCTGAGGGTCTCCGCCGACAGAGCCGCCTTCTACGGCTGCCGGATCATATCCTTTCAAGACACCCTTCTTGACGACATCGGAAAACACTACTTCAAGAACTGTTACATCGAAGGCGCCACCGATTTCATCTGCGGAAACGCCGCTTCTCTCTACGAGAAGTGTCATTTGCACTCAACGTCGGAGAGAGGCGGGGCGATGACGGCACAGCACCGTAACACAGTGGCGGAGAACACTGGGTTCGTGTTCTTGGGGTGCAAAATTACAGGAAGTGGATCGACTTTTCTTGGACGGCCATGGGGGGATTGCTCAAGAGTTGTGTTCGGCTACACTCATATGAGCAACGTTATTGTGCCGGAGGGCTGGAATGACTGGGGAGATCACACCAAGCAAAGAACGGTGTTCTACGGGGAATACAAATGCTACGGACCAGGGGCGAACAGAGCAAAAAGAGTAGCGTGGTCGCGCAGCCTCTCGGCGGATGAGGCTGCAAAACTCTTCACGAAAGACATGATCGGCGGTCGAGGTTGGCTCCGACCTGCTCCGTCTCATTTCAAGAGGAGCTCCTCCACCATTGTCTAG

Coding sequence (CDS)

ATGGCTCCTGTAGATATGAGCACTGCCAGGCTGATCAGGGTAGACCAATCTGGAAAGGGAGATTTTAGGAAGATTCAAGAAGCCATTGATTCTGTTCCATCCCGAAACAATGAACTCGTTTTCATTTGGATCAAACCCGGAACTTATAGGCTGATCAGGGTAGACCAATCTGGAAAGGGAGATTTTAGGAAGATTCAAGAAGCCATTGATTCTGTTCCATCCCGAAACAATGAACTCGTTTTCATTTGGATCAAACCCGGAACTTATAGCAAAGCCTCCGACACCATAATCACATGGAACCAAGGAAGAGATTTACTTCAATCTCCGGCCATCTCTATCTTCGCCGCGGACTTCGTCGGCCGTTTTCTGACCATCCAGAACACATACGGCACCACCGGCATTGCGGTGGCGCTGAGGGTCTCCGCCGACAGAGCCGCCTTCTACGGCTGCCGGATCATATCCTTTCAAGACACCCTTCTTGACGACATCGGAAAACACTACTTCAAGAACTGTTACATCGAAGGCGCCACCGATTTCATCTGCGGAAACGCCGCTTCTCTCTACGAGAAGTGTCATTTGCACTCAACGTCGGAGAGAGGCGGGGCGATGACGGCACAGCACCGTAACACAGTGGCGGAGAACACTGGGTTCGTGTTCTTGGGGTGCAAAATTACAGGAAGTGGATCGACTTTTCTTGGACGGCCATGGGGGGATTGCTCAAGAGTTGTGTTCGGCTACACTCATATGAGCAACGTTATTGTGCCGGAGGGCTGGAATGACTGGGGAGATCACACCAAGCAAAGAACGGTGTTCTACGGGGAATACAAATGCTACGGACCAGGGGCGAACAGAGCAAAAAGAGTAGCGTGGTCGCGCAGCCTCTCGGCGGATGAGGCTGCAAAACTCTTCACGAAAGACATGATCGGCGGTCGAGGTTGGCTCCGACCTGCTCCGTCTCATTTCAAGAGGAGCTCCTCCACCATTGTCTAG

Protein sequence

MAPVDMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFTKDMIGGRGWLRPAPSHFKRSSSTIV
Homology
[Close]
BLAST of Cp4.1LG05g12550 vs. Swiss-Prot
Match: PME11_ARATH (Putative pectinesterase 11 OS=Arabidopsis thaliana GN=PME11 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.1e-113
Identity = 194/323 (60.06%), Postives = 239/323 (73.99%), Query Frame = 1 

Query: 4   VDMSTARLIRVDQSGKGDFRKIQEAIDSVPS--RNNELVFIWIKPGTYRLIRVDQSGKGD 63
           +D STA LIRVDQSGKGDF KIQEAI+S+P    N++L FIW+KPG YR           
Sbjct: 43  IDFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYR----------- 102

Query: 64  FRKIQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGR 123
             K+      +P+    +        + ++AS+T + W+ G D+L+SP ++IFA+DFV R
Sbjct: 103 -EKV-----VIPAEKPYITL------SGTQASNTFLIWSDGEDILESPTLTIFASDFVCR 162

Query: 124 FLTIQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFIC 183
           FLTIQN +GT G AVALRV+AD+AAFYGC I S+QDTLLDD G HYFKNCYIEGATDFIC
Sbjct: 163 FLTIQNKFGTAGRAVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFIC 222

Query: 184 GNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSR 243
           G+A+SLYE+CHLHS S   G++TAQ R +  E +GF FLGCK+TGSGSTFLGRPWG  SR
Sbjct: 223 GSASSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSR 282

Query: 244 VVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAK 303
           VVF Y+  SNV+ P+GWN WGD TK+ TV+YGEYKCYGPGA+R +RV WS+ LS +EA  
Sbjct: 283 VVFAYSFFSNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATV 342

Query: 304 LFTKDMIGGRGWLRPAPSHFKRS 325
             +KD IGG+ WLRPAPSHFK +
Sbjct: 343 FLSKDFIGGKDWLRPAPSHFKNA 342

[Close]
BLAST of Cp4.1LG05g12550 vs. Swiss-Prot
Match: PME53_ARATH (Probable pectinesterase 53 OS=Arabidopsis thaliana GN=PME53 PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.4e-69
Identity = 136/304 (44.74%), Postives = 186/304 (61.18%), Query Frame = 1 

Query: 48  TYRLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSK---------------- 107
           +Y L    +S KGDF KIQ+AIDS+P  N   V I +  G Y +                
Sbjct: 82  SYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGE 141

Query: 108 -ASDTIITWNQGRDLLQSP-------------AISIFAADFVGRFLTIQNTY-----GTT 167
            A  T + W    D  Q+P             + ++ +  FV + +T +NT      G  
Sbjct: 142 GAEKTTVEWG---DTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAV 201

Query: 168 GI-AVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNAASLYEKC 227
           G  AVALRVSAD AAF+GCR++  QDTL D +G+HY+K+CYIEG+ DFI GNA SLYE C
Sbjct: 202 GKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGC 261

Query: 228 HLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVFGYTHMSN 287
           H+H+ +++ GA+TAQ R++V E+TGF F+ CK+TG+G  +LGR WG  SRVVF YT+M N
Sbjct: 262 HVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDN 321

Query: 288 VIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFTKDMIGGR 316
           +I+P GW +WGD +++ TVFYG+YKC G GAN   RVAW+R L+ +EA    +   I G 
Sbjct: 322 IILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGS 381

[Close]
BLAST of Cp4.1LG05g12550 vs. Swiss-Prot
Match: PME62_ARATH (Pectinesterase QRT1 OS=Arabidopsis thaliana GN=QRT1 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.9e-62
Identity = 131/321 (40.81%), Postives = 185/321 (57.63%), Query Frame = 1 

Query: 10  RLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDFRKIQEAI 69
           R+I VD++G GD   +Q A+D VP  N++ V I+I PG YR               ++ I
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYR---------------EKVI 139

Query: 70  DSVPSRNNELVFIWIKPGTYSKASDTIITWNQ--------GRDL--LQSPAISIFAADFV 129
             VP     + FI    G  S A DT+I+W+         G++L   ++ ++SI +  F 
Sbjct: 140 --VPKSKPYISFI----GNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFC 199

Query: 130 GRFLTIQNTY----GTTGI-AVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIE 189
              +T +NT     G  G  AVALR+  D+A FY  R++  QDTL DD G HYF  CYI+
Sbjct: 200 ATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQ 259

Query: 190 GATDFICGNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGR 249
           G  DFI GNA SLY+ C +HST++R GA+ A HR++  E+TGF F+ C I+G+G  +LGR
Sbjct: 260 GNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGR 319

Query: 250 PWGDCSRVVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSL 309
            WG+ SR V+    ++++I P GW+DW    +QR V +GEY C G GA R  RV WS++L
Sbjct: 320 AWGNYSRTVYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTL 379

Query: 310 SADEAAKLFTKDMIGGRGWLR 316
           + DE      ++ I G  WLR
Sbjct: 380 TRDEVKPFLGREFIYGDQWLR 379

[Close]
BLAST of Cp4.1LG05g12550 vs. Swiss-Prot
Match: PME68_ARATH (Probable pectinesterase 68 OS=Arabidopsis thaliana GN=PME68 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.1e-61
Identity = 119/309 (38.51%), Postives = 172/309 (55.66%), Query Frame = 1 

Query: 43  WIKPGTYRLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSK-----ASDTII 102
           W+ P  +++I V  +G   FR +Q+A+DS+P  NN+ + I I PG Y +     A+   I
Sbjct: 52  WVGPSGHKVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYI 111

Query: 103 TWN-QGRDLL---------------------QSPAISIFAADFVGRFLTIQNTY-----G 162
           T+   GRD+                      Q+ +++++A  F  R ++  NT      G
Sbjct: 112 TFKGAGRDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPG 171

Query: 163 TTGI-AVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNAASLYE 222
             G  AVA R+S D+A F GC     QDTL DD G+HYFK CYIEG+ DFI GN  S+Y+
Sbjct: 172 MQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYK 231

Query: 223 KCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVFGYTHM 282
            C LHS + R G++ A  R    E TGF F+GC++TG+G  ++GR  G  SR+V+ YT+ 
Sbjct: 232 DCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYF 291

Query: 283 SNVIVPEGWNDWGDH--TKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFTKDM 317
             ++   GW+DW DH   K +T F+G Y CYGPGA   + V+W+R+L  + A     K  
Sbjct: 292 DALVAHGGWDDW-DHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSF 351

[Close]
BLAST of Cp4.1LG05g12550 vs. Swiss-Prot
Match: PME31_ARATH (Pectinesterase 31 OS=Arabidopsis thaliana GN=PME31 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.1e-59
Identity = 126/312 (40.38%), Postives = 173/312 (55.45%), Query Frame = 1 

Query: 6   MSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDFRKI 65
           M+T R++RV Q G GD+  +Q+AIDSVP  N     I + PG YR               
Sbjct: 1   MATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYR--------------- 60

Query: 66  QEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQ--------------GRDLLQSPAI 125
           Q     VP R N + F  I P        T++TWN               G       ++
Sbjct: 61  QPVY--VPKRKNFITFAGISPEI------TVLTWNNTASKIEHHQASRVIGTGTFGCGSV 120

Query: 126 SIFAADFVGRFLTIQNTYGT-TGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKN 185
            +   DF+   +T +N+    +G AVA+RV+ADR AFY CR + +QDTL    GK Y K+
Sbjct: 121 IVEGEDFIAENITFENSAPEGSGQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKD 180

Query: 186 CYIEGATDFICGNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGST 245
           CYIEG+ DFI GN+ +L E CH+H  S+  G +TAQ R +  E+TG+VFL C ITG+G +
Sbjct: 181 CYIEGSVDFIFGNSTALLEHCHIHCKSQ--GFITAQSRKSSQESTGYVFLRCVITGNGQS 240

Query: 246 ---FLGRPWGDCSRVVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKR 300
              +LGRPWG   RVV  YT+M   I   GW++WG+   +R+  + EY+C+GPG+  ++R
Sbjct: 241 GYMYLGRPWGPFGRVVLAYTYMDACIRNVGWHNWGNAENERSACFYEYRCFGPGSCSSER 287

[Close]
BLAST of Cp4.1LG05g12550 vs. TrEMBL
Match: A0A0A0LP84_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_2G248110 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 8.2e-141
Identity = 251/322 (77.95%), Postives = 271/322 (84.16%), Query Frame = 1 

Query: 1   MAPVDMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKG 60
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTYR          
Sbjct: 3   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYR---------- 62

Query: 61  DFRKIQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVG 120
              KI      VP    +  +I I   + SKASDT ITWNQGRDLL+SP +SIFA+DFVG
Sbjct: 63  --EKI-----VVP---EDKPYITI---SGSKASDTKITWNQGRDLLESPVVSIFASDFVG 122

Query: 121 RFLTIQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFI 180
           RFLTI+NT+GTTGIAVALRVSADRAAFYGCRIISFQDTLLDD G+HYF NCYIEGATDFI
Sbjct: 123 RFLTIENTFGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFI 182

Query: 181 CGNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCS 240
           CGNAASLYEKCHLHSTS+RGGAMTAQHRNT  ENTGFVFLG KITGSGS FLGRPWGD S
Sbjct: 183 CGNAASLYEKCHLHSTSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFS 242

Query: 241 RVVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAA 300
           +VVFGYT+MSNV+ PEGWNDWGD TKQRTV YGEYKCYG GANR KRV WSRSLS DEA+
Sbjct: 243 KVVFGYTYMSNVVEPEGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEAS 301

Query: 301 KLFTKDMIGGRGWLRPAPSHFK 323
           KLFTKD+IGGR WLRPAPSHFK
Sbjct: 303 KLFTKDIIGGRAWLRPAPSHFK 301

[Close]
BLAST of Cp4.1LG05g12550 vs. TrEMBL
Match: W9R2S4_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_018562 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 4.5e-123
Identity = 221/327 (67.58%), Postives = 254/327 (77.68%), Query Frame = 1 

Query: 3   PVDMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDF 62
           P DMSTA LIRVDQ+GKGDF KIQ+AIDSVPS N+ELVFI IKPGTYR            
Sbjct: 13  PTDMSTAILIRVDQTGKGDFGKIQDAIDSVPSNNSELVFISIKPGTYR------------ 72

Query: 63  RKIQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGRF 122
            KI    D          FI +  GT  K+SDTIITWN   ++  SP +S+ A+DFVGR+
Sbjct: 73  EKIVVPADK--------PFITLS-GT--KSSDTIITWNDSGEIFASPTLSVLASDFVGRY 132

Query: 123 LTIQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICG 182
           +TIQNTYGT+G AVALRVS DRAAFY C+I+S+QDTLLDD+GKHYF NC IEGA DFI G
Sbjct: 133 ITIQNTYGTSGKAVALRVSGDRAAFYSCKILSYQDTLLDDVGKHYFNNC-IEGAIDFIFG 192

Query: 183 NAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRV 242
           NAASLYEKCHLHS SER GA+TAQHR + AENTGF FLGC ITG G++FLGRPWG  SRV
Sbjct: 193 NAASLYEKCHLHSLSERDGAITAQHRVSSAENTGFTFLGCNITGVGASFLGRPWGASSRV 252

Query: 243 VFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKL 302
           VF Y HMS+ I+P+GWNDWGD TKQR+V+YGEYKCYGPGANR+KRV WSRSL++DEAA+ 
Sbjct: 253 VFTYCHMSSAIIPQGWNDWGDETKQRSVYYGEYKCYGPGANRSKRVGWSRSLTSDEAAQF 312

Query: 303 FTKDMIGGRGWLRPAPSHFKRSSSTIV 330
            TKDMIGG+ WLR  P HFKR+S  +V
Sbjct: 313 LTKDMIGGKNWLRDPPIHFKRASGALV 315

[Close]
BLAST of Cp4.1LG05g12550 vs. TrEMBL
Match: A5BYJ2_VITVI (Pectinesterase OS=Vitis vinifera GN=VITISV_004378 PE=4 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.3e-117
Identity = 209/325 (64.31%), Postives = 249/325 (76.62%), Query Frame = 1 

Query: 5   DMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDFRK 64
           DMSTA LIRVDQSG GD+ KIQ+AID+VPS N++L FI +KPGTYR             K
Sbjct: 39  DMSTAILIRVDQSGNGDYGKIQDAIDAVPSNNSQLYFILVKPGTYR------------EK 98

Query: 65  IQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLT 124
           I    D          FI +  GT  +AS TIITW  G ++ +SP +SI A+DFVGR+LT
Sbjct: 99  IVVPADK--------PFITLS-GT--QASTTIITWGDGGEIFESPTLSILASDFVGRYLT 158

Query: 125 IQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNA 184
           IQNT+GT+G AVA+RVS DRAAFY CRI+S+QDTLLDD G+HY++NCYIEGATDFICG+A
Sbjct: 159 IQNTFGTSGKAVAVRVSGDRAAFYNCRILSYQDTLLDDAGRHYYRNCYIEGATDFICGSA 218

Query: 185 ASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVF 244
           ASL+EKCHLHS SE  GA+TAQ R + +ENTGF FLGCKITG G+ +LGRPWG  SRVVF
Sbjct: 219 ASLFEKCHLHSLSEGNGAITAQQRGSTSENTGFTFLGCKITGVGTPYLGRPWGPYSRVVF 278

Query: 245 GYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFT 304
             + MS+V+ P+GW+DWGD  KQ TV+YGEYKCYGPGANR +RV WSRSLS+DEA    T
Sbjct: 279 VLSFMSSVVQPQGWDDWGDSNKQSTVYYGEYKCYGPGANRTERVEWSRSLSSDEAVPFLT 338

Query: 305 KDMIGGRGWLRPAPSHFKRSSSTIV 330
           K+MIGG+GWLRPAP+HF R SS IV
Sbjct: 339 KEMIGGQGWLRPAPTHFMRGSSVIV 340

[Close]
BLAST of Cp4.1LG05g12550 vs. TrEMBL
Match: F6I0K6_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_03s0038g04740 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 4.6e-115
Identity = 205/324 (63.27%), Postives = 246/324 (75.93%), Query Frame = 1 

Query: 5   DMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDFRK 64
           DMSTA LIRVDQSG GD+ KIQ+AID+VPS N++L FI +KPGTYR             K
Sbjct: 39  DMSTAILIRVDQSGNGDYGKIQDAIDAVPSNNSQLYFILVKPGTYR------------EK 98

Query: 65  IQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLT 124
           I    D          FI +  GT  +AS TIITW  G ++ +SP +SI A+DFVGR+LT
Sbjct: 99  IVVPADK--------PFITLS-GT--QASTTIITWGDGGEIFESPTLSILASDFVGRYLT 158

Query: 125 IQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNA 184
           IQNT+GT+  AVA+RVS DRAAFY CRI+S+QDTLLDD G+HY++NCYIEGATDFICG+A
Sbjct: 159 IQNTFGTSSKAVAVRVSGDRAAFYNCRILSYQDTLLDDAGRHYYRNCYIEGATDFICGSA 218

Query: 185 ASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVF 244
           ASL+EKCHLHS SE  GA+TAQ R + +EN GF FLGCKITG G+ +LGRPWG  SRVVF
Sbjct: 219 ASLFEKCHLHSLSEGNGAITAQQRGSTSENNGFTFLGCKITGVGTPYLGRPWGPYSRVVF 278

Query: 245 GYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFT 304
             + MS+V+ P+GW+DWGD  KQ TV+YGEYKCYGPGANR +RV WSRSLS+DEA    T
Sbjct: 279 VLSFMSSVVQPQGWDDWGDSNKQSTVYYGEYKCYGPGANRTERVEWSRSLSSDEAVPFLT 338

Query: 305 KDMIGGRGWLRPAPSHFKRSSSTI 329
           K+MIGG+GWLRPAP++F R SS I
Sbjct: 339 KEMIGGQGWLRPAPTYFMRGSSVI 339

[Close]
BLAST of Cp4.1LG05g12550 vs. TrEMBL
Match: A0A061DZ50_THECC (Laccase OS=Theobroma cacao GN=TCM_006868 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.0e-114
Identity = 204/323 (63.16%), Postives = 243/323 (75.23%), Query Frame = 1 

Query: 6   MSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDFRKI 65
           MSTA L+RVDQ+G GDF+KIQ+AID+VPS N+EL FI +KPGTYR             KI
Sbjct: 1   MSTAILLRVDQAGHGDFKKIQDAIDAVPSNNSELYFILVKPGTYR------------EKI 60

Query: 66  QEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTI 125
               D          FI +  GT  +A DTIITWN+   +  S A+++ A+DFVG++LTI
Sbjct: 61  VVPADK--------PFITLS-GT--RAQDTIITWNESGGIFDSAALTVLASDFVGQYLTI 120

Query: 126 QNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNAA 185
           QNT+GT G AVA+RVS DRAAF GCRI+S+QDTLLDD GKHYFKNCYIEG TDFICGNA+
Sbjct: 121 QNTFGTGGKAVAMRVSGDRAAFDGCRILSYQDTLLDDTGKHYFKNCYIEGGTDFICGNAS 180

Query: 186 SLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVFG 245
           S YE CHLHS S + G++TAQHRN+ +ENTGF FLGCKITG G+ FLGRPWG  SRVVF 
Sbjct: 181 SFYEGCHLHSLSTKSGSITAQHRNSPSENTGFTFLGCKITGIGAAFLGRPWGAFSRVVFA 240

Query: 246 YTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFTK 305
            T+MSNVI+P+GW+DW D  KQRT +YGEYKCYGPGA+ +KRV WS  LS +EAA   T 
Sbjct: 241 ETYMSNVIIPQGWDDWQDPRKQRTAYYGEYKCYGPGADASKRVQWSHRLSQNEAAPFLTM 300

Query: 306 DMIGGRGWLRPAPSHFKRSSSTI 329
           DMIGGRGWLRPAP++FKR   +I
Sbjct: 301 DMIGGRGWLRPAPTNFKRRPGSI 300

[Close]
BLAST of Cp4.1LG05g12550 vs. TAIR10
Match: AT2G21610.1 (AT2G21610.1 pectinesterase 11)

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-114
Identity = 194/323 (60.06%), Postives = 239/323 (73.99%), Query Frame = 1 

Query: 4   VDMSTARLIRVDQSGKGDFRKIQEAIDSVPS--RNNELVFIWIKPGTYRLIRVDQSGKGD 63
           +D STA LIRVDQSGKGDF KIQEAI+S+P    N++L FIW+KPG YR           
Sbjct: 43  IDFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYR----------- 102

Query: 64  FRKIQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGR 123
             K+      +P+    +        + ++AS+T + W+ G D+L+SP ++IFA+DFV R
Sbjct: 103 -EKV-----VIPAEKPYITL------SGTQASNTFLIWSDGEDILESPTLTIFASDFVCR 162

Query: 124 FLTIQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFIC 183
           FLTIQN +GT G AVALRV+AD+AAFYGC I S+QDTLLDD G HYFKNCYIEGATDFIC
Sbjct: 163 FLTIQNKFGTAGRAVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFIC 222

Query: 184 GNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSR 243
           G+A+SLYE+CHLHS S   G++TAQ R +  E +GF FLGCK+TGSGSTFLGRPWG  SR
Sbjct: 223 GSASSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSR 282

Query: 244 VVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAK 303
           VVF Y+  SNV+ P+GWN WGD TK+ TV+YGEYKCYGPGA+R +RV WS+ LS +EA  
Sbjct: 283 VVFAYSFFSNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATV 342

Query: 304 LFTKDMIGGRGWLRPAPSHFKRS 325
             +KD IGG+ WLRPAPSHFK +
Sbjct: 343 FLSKDFIGGKDWLRPAPSHFKNA 342

[Close]
BLAST of Cp4.1LG05g12550 vs. TAIR10
Match: AT5G19730.1 (AT5G19730.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 262.7 bits (670), Expect = 3.1e-70
Identity = 136/304 (44.74%), Postives = 186/304 (61.18%), Query Frame = 1 

Query: 48  TYRLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSK---------------- 107
           +Y L    +S KGDF KIQ+AIDS+P  N   V I +  G Y +                
Sbjct: 82  SYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGE 141

Query: 108 -ASDTIITWNQGRDLLQSP-------------AISIFAADFVGRFLTIQNTY-----GTT 167
            A  T + W    D  Q+P             + ++ +  FV + +T +NT      G  
Sbjct: 142 GAEKTTVEWG---DTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAV 201

Query: 168 GI-AVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNAASLYEKC 227
           G  AVALRVSAD AAF+GCR++  QDTL D +G+HY+K+CYIEG+ DFI GNA SLYE C
Sbjct: 202 GKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGC 261

Query: 228 HLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVFGYTHMSN 287
           H+H+ +++ GA+TAQ R++V E+TGF F+ CK+TG+G  +LGR WG  SRVVF YT+M N
Sbjct: 262 HVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDN 321

Query: 288 VIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFTKDMIGGR 316
           +I+P GW +WGD +++ TVFYG+YKC G GAN   RVAW+R L+ +EA    +   I G 
Sbjct: 322 IILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGS 381

[Close]
BLAST of Cp4.1LG05g12550 vs. TAIR10
Match: AT5G55590.1 (AT5G55590.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 240.4 bits (612), Expect = 1.6e-63
Identity = 131/321 (40.81%), Postives = 185/321 (57.63%), Query Frame = 1 

Query: 10  RLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDFRKIQEAI 69
           R+I VD++G GD   +Q A+D VP  N++ V I+I PG YR               ++ I
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYR---------------EKVI 139

Query: 70  DSVPSRNNELVFIWIKPGTYSKASDTIITWNQ--------GRDL--LQSPAISIFAADFV 129
             VP     + FI    G  S A DT+I+W+         G++L   ++ ++SI +  F 
Sbjct: 140 --VPKSKPYISFI----GNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFC 199

Query: 130 GRFLTIQNTY----GTTGI-AVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIE 189
              +T +NT     G  G  AVALR+  D+A FY  R++  QDTL DD G HYF  CYI+
Sbjct: 200 ATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQ 259

Query: 190 GATDFICGNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGR 249
           G  DFI GNA SLY+ C +HST++R GA+ A HR++  E+TGF F+ C I+G+G  +LGR
Sbjct: 260 GNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGR 319

Query: 250 PWGDCSRVVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSL 309
            WG+ SR V+    ++++I P GW+DW    +QR V +GEY C G GA R  RV WS++L
Sbjct: 320 AWGNYSRTVYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTL 379

Query: 310 SADEAAKLFTKDMIGGRGWLR 316
           + DE      ++ I G  WLR
Sbjct: 380 TRDEVKPFLGREFIYGDQWLR 379

[Close]
BLAST of Cp4.1LG05g12550 vs. TAIR10
Match: AT5G47500.1 (AT5G47500.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 235.7 bits (600), Expect = 4.0e-62
Identity = 119/309 (38.51%), Postives = 172/309 (55.66%), Query Frame = 1 

Query: 43  WIKPGTYRLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSK-----ASDTII 102
           W+ P  +++I V  +G   FR +Q+A+DS+P  NN+ + I I PG Y +     A+   I
Sbjct: 52  WVGPSGHKVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYI 111

Query: 103 TWN-QGRDLL---------------------QSPAISIFAADFVGRFLTIQNTY-----G 162
           T+   GRD+                      Q+ +++++A  F  R ++  NT      G
Sbjct: 112 TFKGAGRDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPG 171

Query: 163 TTGI-AVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNAASLYE 222
             G  AVA R+S D+A F GC     QDTL DD G+HYFK CYIEG+ DFI GN  S+Y+
Sbjct: 172 MQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYK 231

Query: 223 KCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVFGYTHM 282
            C LHS + R G++ A  R    E TGF F+GC++TG+G  ++GR  G  SR+V+ YT+ 
Sbjct: 232 DCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYF 291

Query: 283 SNVIVPEGWNDWGDH--TKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFTKDM 317
             ++   GW+DW DH   K +T F+G Y CYGPGA   + V+W+R+L  + A     K  
Sbjct: 292 DALVAHGGWDDW-DHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSF 351

[Close]
BLAST of Cp4.1LG05g12550 vs. TAIR10
Match: AT3G29090.1 (AT3G29090.1 pectin methylesterase 31)

HSP 1 Score: 229.6 bits (584), Expect = 2.9e-60
Identity = 126/312 (40.38%), Postives = 173/312 (55.45%), Query Frame = 1 

Query: 6   MSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDFRKI 65
           M+T R++RV Q G GD+  +Q+AIDSVP  N     I + PG YR               
Sbjct: 1   MATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYR--------------- 60

Query: 66  QEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQ--------------GRDLLQSPAI 125
           Q     VP R N + F  I P        T++TWN               G       ++
Sbjct: 61  QPVY--VPKRKNFITFAGISPEI------TVLTWNNTASKIEHHQASRVIGTGTFGCGSV 120

Query: 126 SIFAADFVGRFLTIQNTYGT-TGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKN 185
            +   DF+   +T +N+    +G AVA+RV+ADR AFY CR + +QDTL    GK Y K+
Sbjct: 121 IVEGEDFIAENITFENSAPEGSGQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKD 180

Query: 186 CYIEGATDFICGNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGST 245
           CYIEG+ DFI GN+ +L E CH+H  S+  G +TAQ R +  E+TG+VFL C ITG+G +
Sbjct: 181 CYIEGSVDFIFGNSTALLEHCHIHCKSQ--GFITAQSRKSSQESTGYVFLRCVITGNGQS 240

Query: 246 ---FLGRPWGDCSRVVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKR 300
              +LGRPWG   RVV  YT+M   I   GW++WG+   +R+  + EY+C+GPG+  ++R
Sbjct: 241 GYMYLGRPWGPFGRVVLAYTYMDACIRNVGWHNWGNAENERSACFYEYRCFGPGSCSSER 287

[Close]
BLAST of Cp4.1LG05g12550 vs. NCBI nr
Match: gi|449462266|ref|XP_004148862.1| (PREDICTED: putative pectinesterase 11 [Cucumis sativus])

HSP 1 Score: 508.1 bits (1307), Expect = 1.2e-140
Identity = 251/322 (77.95%), Postives = 271/322 (84.16%), Query Frame = 1 

Query: 1   MAPVDMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKG 60
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTYR          
Sbjct: 12  MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYR---------- 71

Query: 61  DFRKIQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVG 120
              KI      VP    +  +I I   + SKASDT ITWNQGRDLL+SP +SIFA+DFVG
Sbjct: 72  --EKI-----VVP---EDKPYITI---SGSKASDTKITWNQGRDLLESPVVSIFASDFVG 131

Query: 121 RFLTIQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFI 180
           RFLTI+NT+GTTGIAVALRVSADRAAFYGCRIISFQDTLLDD G+HYF NCYIEGATDFI
Sbjct: 132 RFLTIENTFGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFI 191

Query: 181 CGNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCS 240
           CGNAASLYEKCHLHSTS+RGGAMTAQHRNT  ENTGFVFLG KITGSGS FLGRPWGD S
Sbjct: 192 CGNAASLYEKCHLHSTSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFS 251

Query: 241 RVVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAA 300
           +VVFGYT+MSNV+ PEGWNDWGD TKQRTV YGEYKCYG GANR KRV WSRSLS DEA+
Sbjct: 252 KVVFGYTYMSNVVEPEGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEAS 310

Query: 301 KLFTKDMIGGRGWLRPAPSHFK 323
           KLFTKD+IGGR WLRPAPSHFK
Sbjct: 312 KLFTKDIIGGRAWLRPAPSHFK 310

[Close]
BLAST of Cp4.1LG05g12550 vs. NCBI nr
Match: gi|700206682|gb|KGN61801.1| (hypothetical protein Csa_2G248110 [Cucumis sativus])

HSP 1 Score: 508.1 bits (1307), Expect = 1.2e-140
Identity = 251/322 (77.95%), Postives = 271/322 (84.16%), Query Frame = 1 

Query: 1   MAPVDMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKG 60
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTYR          
Sbjct: 3   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYR---------- 62

Query: 61  DFRKIQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVG 120
              KI      VP    +  +I I   + SKASDT ITWNQGRDLL+SP +SIFA+DFVG
Sbjct: 63  --EKI-----VVP---EDKPYITI---SGSKASDTKITWNQGRDLLESPVVSIFASDFVG 122

Query: 121 RFLTIQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFI 180
           RFLTI+NT+GTTGIAVALRVSADRAAFYGCRIISFQDTLLDD G+HYF NCYIEGATDFI
Sbjct: 123 RFLTIENTFGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFI 182

Query: 181 CGNAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCS 240
           CGNAASLYEKCHLHSTS+RGGAMTAQHRNT  ENTGFVFLG KITGSGS FLGRPWGD S
Sbjct: 183 CGNAASLYEKCHLHSTSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFS 242

Query: 241 RVVFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAA 300
           +VVFGYT+MSNV+ PEGWNDWGD TKQRTV YGEYKCYG GANR KRV WSRSLS DEA+
Sbjct: 243 KVVFGYTYMSNVVEPEGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEAS 301

Query: 301 KLFTKDMIGGRGWLRPAPSHFK 323
           KLFTKD+IGGR WLRPAPSHFK
Sbjct: 303 KLFTKDIIGGRAWLRPAPSHFK 301

[Close]
BLAST of Cp4.1LG05g12550 vs. NCBI nr
Match: gi|659122048|ref|XP_008460943.1| (PREDICTED: LOW QUALITY PROTEIN: putative pectinesterase 11 [Cucumis melo])

HSP 1 Score: 507.3 bits (1305), Expect = 2.0e-140
Identity = 250/320 (78.12%), Postives = 269/320 (84.06%), Query Frame = 1 

Query: 3   PVDMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDF 62
           P D  TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTYR            
Sbjct: 4   PRDKRTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYR------------ 63

Query: 63  RKIQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGRF 122
            KI      VP    +  +I I   + SKASDTIITWNQGRDLLQSP +SIFA+DFVGRF
Sbjct: 64  EKI-----VVP---EDKPYITI---SGSKASDTIITWNQGRDLLQSPVVSIFASDFVGRF 123

Query: 123 LTIQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICG 182
           LTI+NT+GT+GIAVALRVSADRAAFYGCRIISFQDTLLDD G+HYF NCYIEGATDFICG
Sbjct: 124 LTIENTFGTSGIAVALRVSADRAAFYGCRIISFQDTLLDDSGRHYFNNCYIEGATDFICG 183

Query: 183 NAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRV 242
           NAASLYE+CHLHSTS+RGGAMTAQHRNT  ENTGFVFLG KITGSGS FLGRPWG  S+V
Sbjct: 184 NAASLYERCHLHSTSDRGGAMTAQHRNTAEENTGFVFLGGKITGSGSMFLGRPWGGFSKV 243

Query: 243 VFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKL 302
           VFGYT+MSNV+ PEGWNDWGD TKQRTV YGEYKCYGPGANRAKRV WSRSLS DEA KL
Sbjct: 244 VFGYTYMSNVVEPEGWNDWGDPTKQRTVSYGEYKCYGPGANRAKRVVWSRSLSTDEATKL 300

Query: 303 FTKDMIGGRGWLRPAPSHFK 323
           FTKD+IGGR WLRPAPSHFK
Sbjct: 304 FTKDIIGGRAWLRPAPSHFK 300

[Close]
BLAST of Cp4.1LG05g12550 vs. NCBI nr
Match: gi|703071053|ref|XP_010088935.1| (Putative pectinesterase 11 [Morus notabilis])

HSP 1 Score: 449.1 bits (1154), Expect = 6.5e-123
Identity = 221/327 (67.58%), Postives = 254/327 (77.68%), Query Frame = 1 

Query: 3   PVDMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDF 62
           P DMSTA LIRVDQ+GKGDF KIQ+AIDSVPS N+ELVFI IKPGTYR            
Sbjct: 13  PTDMSTAILIRVDQTGKGDFGKIQDAIDSVPSNNSELVFISIKPGTYR------------ 72

Query: 63  RKIQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGRF 122
            KI    D          FI +  GT  K+SDTIITWN   ++  SP +S+ A+DFVGR+
Sbjct: 73  EKIVVPADK--------PFITLS-GT--KSSDTIITWNDSGEIFASPTLSVLASDFVGRY 132

Query: 123 LTIQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICG 182
           +TIQNTYGT+G AVALRVS DRAAFY C+I+S+QDTLLDD+GKHYF NC IEGA DFI G
Sbjct: 133 ITIQNTYGTSGKAVALRVSGDRAAFYSCKILSYQDTLLDDVGKHYFNNC-IEGAIDFIFG 192

Query: 183 NAASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRV 242
           NAASLYEKCHLHS SER GA+TAQHR + AENTGF FLGC ITG G++FLGRPWG  SRV
Sbjct: 193 NAASLYEKCHLHSLSERDGAITAQHRVSSAENTGFTFLGCNITGVGASFLGRPWGASSRV 252

Query: 243 VFGYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKL 302
           VF Y HMS+ I+P+GWNDWGD TKQR+V+YGEYKCYGPGANR+KRV WSRSL++DEAA+ 
Sbjct: 253 VFTYCHMSSAIIPQGWNDWGDETKQRSVYYGEYKCYGPGANRSKRVGWSRSLTSDEAAQF 312

Query: 303 FTKDMIGGRGWLRPAPSHFKRSSSTIV 330
            TKDMIGG+ WLR  P HFKR+S  +V
Sbjct: 313 LTKDMIGGKNWLRDPPIHFKRASGALV 315

[Close]
BLAST of Cp4.1LG05g12550 vs. NCBI nr
Match: gi|147843788|emb|CAN79458.1| (hypothetical protein VITISV_004378 [Vitis vinifera])

HSP 1 Score: 431.0 bits (1107), Expect = 1.8e-117
Identity = 209/325 (64.31%), Postives = 249/325 (76.62%), Query Frame = 1 

Query: 5   DMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYRLIRVDQSGKGDFRK 64
           DMSTA LIRVDQSG GD+ KIQ+AID+VPS N++L FI +KPGTYR             K
Sbjct: 39  DMSTAILIRVDQSGNGDYGKIQDAIDAVPSNNSQLYFILVKPGTYR------------EK 98

Query: 65  IQEAIDSVPSRNNELVFIWIKPGTYSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLT 124
           I    D          FI +  GT  +AS TIITW  G ++ +SP +SI A+DFVGR+LT
Sbjct: 99  IVVPADK--------PFITLS-GT--QASTTIITWGDGGEIFESPTLSILASDFVGRYLT 158

Query: 125 IQNTYGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNA 184
           IQNT+GT+G AVA+RVS DRAAFY CRI+S+QDTLLDD G+HY++NCYIEGATDFICG+A
Sbjct: 159 IQNTFGTSGKAVAVRVSGDRAAFYNCRILSYQDTLLDDAGRHYYRNCYIEGATDFICGSA 218

Query: 185 ASLYEKCHLHSTSERGGAMTAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVF 244
           ASL+EKCHLHS SE  GA+TAQ R + +ENTGF FLGCKITG G+ +LGRPWG  SRVVF
Sbjct: 219 ASLFEKCHLHSLSEGNGAITAQQRGSTSENTGFTFLGCKITGVGTPYLGRPWGPYSRVVF 278

Query: 245 GYTHMSNVIVPEGWNDWGDHTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFT 304
             + MS+V+ P+GW+DWGD  KQ TV+YGEYKCYGPGANR +RV WSRSLS+DEA    T
Sbjct: 279 VLSFMSSVVQPQGWDDWGDSNKQSTVYYGEYKCYGPGANRTERVEWSRSLSSDEAVPFLT 338

Query: 305 KDMIGGRGWLRPAPSHFKRSSSTIV 330
           K+MIGG+GWLRPAP+HF R SS IV
Sbjct: 339 KEMIGGQGWLRPAPTHFMRGSSVIV 340

The following BLAST results are available for this feature:
BLAST of Cp4.1LG05g12550 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME11_ARATH2.1e-11360.06Putative pectinesterase 11 OS=Arabidopsis thaliana GN=PME11 PE=3 SV=1[more]
PME53_ARATH5.4e-6944.74Probable pectinesterase 53 OS=Arabidopsis thaliana GN=PME53 PE=2 SV=1[more]
PME62_ARATH2.9e-6240.81Pectinesterase QRT1 OS=Arabidopsis thaliana GN=QRT1 PE=2 SV=1[more]
PME68_ARATH7.1e-6138.51Probable pectinesterase 68 OS=Arabidopsis thaliana GN=PME68 PE=2 SV=1[more]
PME31_ARATH5.1e-5940.38Pectinesterase 31 OS=Arabidopsis thaliana GN=PME31 PE=1 SV=1[more]
BLAST of Cp4.1LG05g12550 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LP84_CUCSA8.2e-14177.95Pectinesterase OS=Cucumis sativus GN=Csa_2G248110 PE=4 SV=1[more]
W9R2S4_9ROSA4.5e-12367.58Pectinesterase OS=Morus notabilis GN=L484_018562 PE=4 SV=1[more]
A5BYJ2_VITVI1.3e-11764.31Pectinesterase OS=Vitis vinifera GN=VITISV_004378 PE=4 SV=1[more]
F6I0K6_VITVI4.6e-11563.27Pectinesterase OS=Vitis vinifera GN=VIT_03s0038g04740 PE=4 SV=1[more]
A0A061DZ50_THECC3.0e-11463.16Laccase OS=Theobroma cacao GN=TCM_006868 PE=3 SV=1[more]
BLAST of Cp4.1LG05g12550 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G21610.11.2e-11460.06 pectinesterase 11[more]
AT5G19730.13.1e-7044.74 Pectin lyase-like superfamily protein[more]
AT5G55590.11.6e-6340.81 Pectin lyase-like superfamily protein[more]
AT5G47500.14.0e-6238.51 Pectin lyase-like superfamily protein[more]
AT3G29090.12.9e-6040.38 pectin methylesterase 31[more]
BLAST of Cp4.1LG05g12550 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449462266|ref|XP_004148862.1|1.2e-14077.95PREDICTED: putative pectinesterase 11 [Cucumis sativus][more]
gi|700206682|gb|KGN61801.1|1.2e-14077.95hypothetical protein Csa_2G248110 [Cucumis sativus][more]
gi|659122048|ref|XP_008460943.1|2.0e-14078.13PREDICTED: LOW QUALITY PROTEIN: putative pectinesterase 11 [Cucumis melo][more]
gi|703071053|ref|XP_010088935.1|6.5e-12367.58Putative pectinesterase 11 [Morus notabilis][more]
gi|147843788|emb|CAN79458.1|1.8e-11764.31hypothetical protein VITISV_004378 [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g12550.1Cp4.1LG05g12550.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 101..305
score: 6.2
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 8..50
score: 4.08E-10coord: 51..321
score: 4.71
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 52..318
score: 8.7E-90coord: 11..50
score: 4.2
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 51..325
score: 9.3E
NoneNo IPR availablePANTHERPTHR31321:SF10PECTINESTERASE 11-RELATEDcoord: 51..325
score: 9.3E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG05g12550Cucsa.109560Cucumber (Gy14) v1cgycpeB0247
Cp4.1LG05g12550CmoCh02G003990Cucurbita moschata (Rifu)cmocpeB595
Cp4.1LG05g12550Cla016565Watermelon (97103) v1cpewmB751
Cp4.1LG05g12550Csa2G248110Cucumber (Chinese Long) v2cpecuB704
Cp4.1LG05g12550MELO3C023302Melon (DHL92) v3.5.1cpemeB656
Cp4.1LG05g12550ClCG11G004340Watermelon (Charleston Gray)cpewcgB634
Cp4.1LG05g12550CSPI02G12270Wild cucumber (PI 183967)cpecpiB706
Cp4.1LG05g12550Lsi10G003750Bottle gourd (USVL1VR-Ls)cpelsiB589
Cp4.1LG05g12550MELO3C023302.2Melon (DHL92) v3.6.1cpemedB786
Cp4.1LG05g12550CsaV3_2G014800Cucumber (Chinese Long) v3cpecucB0879
Cp4.1LG05g12550Bhi10G001608Wax gourdcpewgoB0958
Cp4.1LG05g12550CsGy2G012280Cucumber (Gy14) v2cgybcpeB240
Cp4.1LG05g12550Carg22941Silver-seed gourdcarcpeB1243
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG05g12550Cp4.1LG07g04090Cucurbita pepo (Zucchini)cpecpeB506
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG05g12550Cucurbita pepo (Zucchini)cpecpeB274
Cp4.1LG05g12550Cucurbita pepo (Zucchini)cpecpeB309
Cp4.1LG05g12550Cucurbita pepo (Zucchini)cpecpeB496
Cp4.1LG05g12550Cucurbita maxima (Rimu)cmacpeB585
Cp4.1LG05g12550Cucurbita maxima (Rimu)cmacpeB646
Cp4.1LG05g12550Cucurbita moschata (Rifu)cmocpeB499
Cp4.1LG05g12550Cucurbita moschata (Rifu)cmocpeB534
Cp4.1LG05g12550Bottle gourd (USVL1VR-Ls)cpelsiB605
Cp4.1LG05g12550Watermelon (Charleston Gray)cpewcgB662
Cp4.1LG05g12550Watermelon (97103) v1cpewmB748
Cp4.1LG05g12550Melon (DHL92) v3.5.1cpemeB684
Cp4.1LG05g12550Cucumber (Gy14) v2cgybcpeB974
Cp4.1LG05g12550Melon (DHL92) v3.6.1cpemedB816
Cp4.1LG05g12550Silver-seed gourdcarcpeB0660
Cp4.1LG05g12550Silver-seed gourdcarcpeB0255
Cp4.1LG05g12550Silver-seed gourdcarcpeB0440
Cp4.1LG05g12550Cucumber (Chinese Long) v3cpecucB0898
Cp4.1LG05g12550Cucumber (Chinese Long) v3cpecucB0947
Cp4.1LG05g12550Wax gourdcpewgoB0932