Cp4.1LG05g06970 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG05g06970
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionGibberellin 20-oxidase
LocationCp4.1LG05 : 4291819 .. 4293264 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGTCGTGACATCCACGCCGGAATCCCGACACGACGGCGCTCCATTAGTGTTCGACGCCTCGGTTCTCCGACACCAACACAACATTCCGAAGCAGTTCATATGGCCCGACGAGGAGAAGCCGGCTACCACCTGCCCGGAGCTGGAAGTCCCATTGATCGACTTGTCGGGATTTCTCTCCGGCGAGAAAGATGCGGCGGCGGAGGCTGTTCGACTTGTCGGCGAGGCTTGTGAGAAACATGGGTTTTTTCTGGTGGTGAACCATGGCGTGGACAGGAAATTGATCGGTGAGGCTCATAAGTATATGGATGAGTTCTTTGAATTGCCGCTTTGCCAAAAACAGAGCGCTCAGAGGAAGGCCGGTGAGCACTGTGGGTATGCCAGCAGCTTCACCGGTCGTTTCTCTTCCAAATTGCCTTGGAAAGAGACCCTTTCATTTCGATTCTCCGCCGATGAATCCTTCAATAATCTTGTTCTTCATTACCTTCATGACAAGCTCGGTGACCAATTCGCCAAATTTGGGTACTTTCCGCTAACCCGTCTTCTCTTTTTACGGTTCGATTTGAATCTATCATCTTTATAATGTCGATGTTCTTCTCTAAAATTTGATTTCATCAAATGGGTTTCTTTTTTTACTCTTAAATTTTGATTTCATCAAATGGGTTTCTCTTTTTACGCTTCGATTTGACTCTATAATCTTTATAATGTCGATATTCTTCTCTCAAATTTGATTTCATCAAAAGGGTCTTTTATCTTTTTTTTTTTTTCACTTTTTATGGTTTGATTTGAATCTATAATCTTTATAACATCGATATTCTTCTCTCGAATTTGATTTCATCAAATGGGTTTCTCGTTTTACGCTTCGATTTGAATCTATAATCTTTATAACGTTGACATTCTTCTCTCAAATTGGATTTCATCAAATGGGTATTTGTTTTTTTGATTCGATTCAACTTTAAGATCAAAACTCAAAAGTGGGTTTATGTTGATTGTATCCAAAACAGTGAGATTATGGGGATTGATTTAGTGGAAGGATATTGATATTGCAATTTGTGTGTGTAACAGTAGGGTTTACCAAGAGTATTGTGAAGCAATGAGTGGGCTGTCCTTGGGCATAATGGAGCTATTGGGGAAGAGCCTTGGAGTAGAAGAACAATACTTCAAAAACTTCTTCAAAGACAATGATTCCATAATGAGATTGAACTTCTACCCGCCATGCCAAAAGCCCCATCTCACCTTAGGCACTGGCCCTCACTGCGACCCAACCTCCTTGACCATCCTCCACCAAGACCAAGTCGGTGGCTTACAAGTGTTCGTCGACAACCAATGGAGACTCATCACCCCCAACTTCGACGCTTTCGTCGTCAACATCGGCGACACATTCATGGTAACTAATCAATCAATCAAACAAACAAATCCAAACCCATTTCCTGATACGTCTTAA

mRNA sequence

ATGCATGTCGTGACATCCACGCCGGAATCCCGACACGACGGCGCTCCATTAGTGTTCGACGCCTCGGTTCTCCGACACCAACACAACATTCCGAAGCAGTTCATATGGCCCGACGAGGAGAAGCCGGCTACCACCTGCCCGGAGCTGGAAGTCCCATTGATCGACTTGTCGGGATTTCTCTCCGGCGAGAAAGATGCGGCGGCGGAGGCTGTTCGACTTGTCGGCGAGGCTTGTGAGAAACATGGGTTTTTTCTGGTGGTGAACCATGGCGTGGACAGGAAATTGATCGGTGAGGCTCATAAGTATATGGATGAGTTCTTTGAATTGCCGCTTTGCCAAAAACAGAGCGCTCAGAGGAAGGCCGGTGAGCACTGTGGGTATGCCAGCAGCTTCACCGGTCGTTTCTCTTCCAAATTGCCTTGGAAAGAGACCCTTTCATTTCGATTCTCCGCCGATGAATCCTTCAATAATCTTGTTCTTCATTACCTTCATGACAAGCTCGGTGACCAATTCGCCAAATTTGGTAGGGTTTACCAAGAGTATTGTGAAGCAATGAGTGGGCTGTCCTTGGGCATAATGGAGCTATTGGGGAAGAGCCTTGGAGTAGAAGAACAATACTTCAAAAACTTCTTCAAAGACAATGATTCCATAATGAGATTGAACTTCTACCCGCCATGCCAAAAGCCCCATCTCACCTTAGGCACTGGCCCTCACTGCGACCCAACCTCCTTGACCATCCTCCACCAAGACCAAGTCGGTGGCTTACAAGTGTTCGTCGACAACCAATGGAGACTCATCACCCCCAACTTCGACGCTTTCGTCGTCAACATCGGCGACACATTCATGGTAACTAATCAATCAATCAAACAAACAAATCCAAACCCATTTCCTGATACGTCTTAA

Coding sequence (CDS)

ATGCATGTCGTGACATCCACGCCGGAATCCCGACACGACGGCGCTCCATTAGTGTTCGACGCCTCGGTTCTCCGACACCAACACAACATTCCGAAGCAGTTCATATGGCCCGACGAGGAGAAGCCGGCTACCACCTGCCCGGAGCTGGAAGTCCCATTGATCGACTTGTCGGGATTTCTCTCCGGCGAGAAAGATGCGGCGGCGGAGGCTGTTCGACTTGTCGGCGAGGCTTGTGAGAAACATGGGTTTTTTCTGGTGGTGAACCATGGCGTGGACAGGAAATTGATCGGTGAGGCTCATAAGTATATGGATGAGTTCTTTGAATTGCCGCTTTGCCAAAAACAGAGCGCTCAGAGGAAGGCCGGTGAGCACTGTGGGTATGCCAGCAGCTTCACCGGTCGTTTCTCTTCCAAATTGCCTTGGAAAGAGACCCTTTCATTTCGATTCTCCGCCGATGAATCCTTCAATAATCTTGTTCTTCATTACCTTCATGACAAGCTCGGTGACCAATTCGCCAAATTTGGTAGGGTTTACCAAGAGTATTGTGAAGCAATGAGTGGGCTGTCCTTGGGCATAATGGAGCTATTGGGGAAGAGCCTTGGAGTAGAAGAACAATACTTCAAAAACTTCTTCAAAGACAATGATTCCATAATGAGATTGAACTTCTACCCGCCATGCCAAAAGCCCCATCTCACCTTAGGCACTGGCCCTCACTGCGACCCAACCTCCTTGACCATCCTCCACCAAGACCAAGTCGGTGGCTTACAAGTGTTCGTCGACAACCAATGGAGACTCATCACCCCCAACTTCGACGCTTTCGTCGTCAACATCGGCGACACATTCATGGTAACTAATCAATCAATCAAACAAACAAATCCAAACCCATTTCCTGATACGTCTTAA

Protein sequence

MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMVTNQSIKQTNPNPFPDTS
Homology
[Close]
BLAST of Cp4.1LG05g06970 vs. Swiss-Prot
Match: GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 2.5e-105
Identity = 182/274 (66.42%), Postives = 209/274 (76.28%), Query Frame = 1 

Query: 15  APLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAAEAVRLV 74
           +PL+F+ S+L  Q  IP QFIWPDEEKP+   PEL VP IDLS      +D+  EA R++
Sbjct: 27  SPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDLSS-----QDSTLEAPRVI 86

Query: 75  GEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGR 134
            EAC KHGFFLVVNHGV   LI +AH+ M+ FF++PL  KQ AQRK GE CGYASSFTGR
Sbjct: 87  AEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGR 146

Query: 135 FSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIME 194
           FS+KLPWKETLSF+FS D S +  V  Y  D LG +F +FG+VYQ+YCEAMS LSL IME
Sbjct: 147 FSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIME 206

Query: 195 LLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGG 254
           LLG SLGV   YF+ FF++NDSIMRLN YPPCQ P LTLGTGPHCDP+SLTILHQD V G
Sbjct: 207 LLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNG 266

Query: 255 LQVFVDNQWRLITPNFDAFVVNIGDTFMVTNQSI 289
           LQVFVDNQW+ I PN  AFVVNIGDTFM  +  I
Sbjct: 267 LQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGI 295

[Close]
BLAST of Cp4.1LG05g06970 vs. Swiss-Prot
Match: GAOX3_ARATH (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 8.9e-103
Identity = 179/275 (65.09%), Postives = 216/275 (78.55%), Query Frame = 1 

Query: 18  VFDASVL-RHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAAEAVRLVGE 77
           +FDA +L +H H+IP+QF+WPD EKP+T    L+VPLIDL+GFLSG+   A+EA RLV +
Sbjct: 24  IFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSK 83

Query: 78  ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
           A  KHGFFL+ NHGVD  L+  A+ +MD FF+ P C+KQ AQRK GE  GYASSF GRFS
Sbjct: 84  AATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFS 143

Query: 138 SKLPWKETLSFRFSADESFNN-LVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIMEL 197
           SKLPWKETLSF+FS +E  ++  V  ++  K+GD +  FG+VYQEY EAM+ LSL IMEL
Sbjct: 144 SKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMEL 203

Query: 198 LGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGL 257
           LG SLGVE +YFK FF+D+DSI RLN+YP C++P L LGTGPHCDPTSLTILHQDQVGGL
Sbjct: 204 LGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGL 263

Query: 258 QVFVDNQWRLITPNFDAFVVNIGDTFM-VTNQSIK 290
           QVFVDN+W+ I PN  AFVVNIGDTFM +TN   K
Sbjct: 264 QVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYK 298

[Close]
BLAST of Cp4.1LG05g06970 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 374.8 bits (961), Expect = 8.9e-103
Identity = 179/267 (67.04%), Postives = 207/267 (77.53%), Query Frame = 1 

Query: 16  PLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAAEAVRLVG 75
           PL+F+ S+L  Q NIP QFIWPD+EKP+    EL+VPLIDL   LS +  +  +A RL+ 
Sbjct: 26  PLIFNPSMLNLQANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLIS 85

Query: 76  EACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRF 135
           EAC+KHGFFLVVNHG+  +LI +AH+Y   FF++PL +KQ   RK+GE  GYASSFTGRF
Sbjct: 86  EACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRF 145

Query: 136 SSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIMEL 195
           S+KLPWKETLSFRF  D S +  V  Y  D LG  F  FG+VYQEYCEAMS LSL IMEL
Sbjct: 146 STKLPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMEL 205

Query: 196 LGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGL 255
           LG SLGV+  YF+ FF++NDSIMRLN+YPPC KP LTLGTGPHCDPTSLTILHQD V GL
Sbjct: 206 LGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGL 265

Query: 256 QVFVDNQWRLITPNFDAFVVNIGDTFM 283
           QVFV+NQWR I PN  AFVVNIGDTFM
Sbjct: 266 QVFVENQWRSIRPNPKAFVVNIGDTFM 291

[Close]
BLAST of Cp4.1LG05g06970 vs. Swiss-Prot
Match: GAO1D_WHEAT (Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.7e-93
Identity = 166/266 (62.41%), Postives = 197/266 (74.06%), Query Frame = 1 

Query: 18  VFDASVLRHQHNIPKQFIWPDEEKPA-TTCPELEVPLIDLSGFLSGEKDAAAEAVRLVGE 77
           VFDA+VL  + +IP QFIWP+ E P      EL VPLID+ G LSG+  AAAE  RLVGE
Sbjct: 5   VFDAAVLSGRADIPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPAAAAEVTRLVGE 64

Query: 78  ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
           ACE+HGFF VVNHG+D +L+ +AH+ +D FF +PL +KQ A R  GE CGYASSFTGRF+
Sbjct: 65  ACERHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRHPGESCGYASSFTGRFA 124

Query: 138 SKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIMELL 197
           SKLPWKETLSFR    +    LV+ Y+   LG+   + G VY  YC  MS LSL IME+L
Sbjct: 125 SKLPWKETLSFRSCPSDP--ALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVL 184

Query: 198 GKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGLQ 257
           G+SLGV   +++ FF+ NDSIMRLN+YPPCQ+P  TLGTGPHCDPTSLTILHQD VGGLQ
Sbjct: 185 GESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQ 244

Query: 258 VFVDNQWRLITPNFDAFVVNIGDTFM 283
           V  + +WR I P  DAFVVNIGDTFM
Sbjct: 245 VHTEGRWRSIRPRADAFVVNIGDTFM 268

[Close]
BLAST of Cp4.1LG05g06970 vs. Swiss-Prot
Match: GAO1A_WHEAT (Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 2.2e-93
Identity = 165/266 (62.03%), Postives = 198/266 (74.44%), Query Frame = 1 

Query: 18  VFDASVLRHQHNIPKQFIWPDEEKPA-TTCPELEVPLIDLSGFLSGEKDAAAEAVRLVGE 77
           VFDA+VL  + +IP QFIWP+ E P      EL VPLI++ G LSG+  AAAE  RLVGE
Sbjct: 5   VFDAAVLSGRSDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGE 64

Query: 78  ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
           ACE+HGFF VVNHG+D +L+ +AH+ +D FF +PL +KQ A R+ GE CGYASSFTGRF+
Sbjct: 65  ACERHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFA 124

Query: 138 SKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIMELL 197
           SKLPWKETLSFR    +    LV+ Y+   LG+   + G VY  YC  MS LSL IME+L
Sbjct: 125 SKLPWKETLSFRSCPSDP--ALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVL 184

Query: 198 GKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGLQ 257
           G+SLGV   +++ FF+ NDSIMRLN+YPPCQ+P  TLGTGPHCDPTSLTILHQD VGGLQ
Sbjct: 185 GESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQ 244

Query: 258 VFVDNQWRLITPNFDAFVVNIGDTFM 283
           V  + +WR I P  DAFVVNIGDTFM
Sbjct: 245 VHTEGRWRSIRPRADAFVVNIGDTFM 268

[Close]
BLAST of Cp4.1LG05g06970 vs. TrEMBL
Match: Q8GT56_CUCMA (Gibberellin 20-oxidase OS=Cucurbita maxima GN=ga20ox PE=2 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 3.5e-162
Identity = 274/282 (97.16%), Postives = 278/282 (98.58%), Query Frame = 1 

Query: 1   MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFL 60
           MHVVTSTPE+RHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPA TCPELEVPLIDLSGFL
Sbjct: 1   MHVVTSTPEARHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELEVPLIDLSGFL 60

Query: 61  SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
           SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPL QKQSAQRK
Sbjct: 61  SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLSQKQSAQRK 120

Query: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQE 180
           AGEHCGYASSFTGRFSSKLPWKETLSFRF+ADES NNLVLHYL+DKLGDQFAKFGRVYQ+
Sbjct: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFAADESLNNLVLHYLNDKLGDQFAKFGRVYQD 180

Query: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
           YCEAMSGLSLGIMELLGKSLGVEEQ FKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD
Sbjct: 181 YCEAMSGLSLGIMELLGKSLGVEEQCFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240

Query: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 283
           PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM
Sbjct: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 282

[Close]
BLAST of Cp4.1LG05g06970 vs. TrEMBL
Match: K7ZU06_CUCSA (Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox1 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 1.2e-143
Identity = 246/286 (86.01%), Postives = 260/286 (90.91%), Query Frame = 1 

Query: 1   MHVVTST---PESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATT-CPELEVPLIDL 60
           MHVVTS+     + +DG PLVFDASVLRHQH IPKQFIWPDEEKP+   CPE+EVPLIDL
Sbjct: 1   MHVVTSSMAKEAANNDGVPLVFDASVLRHQHKIPKQFIWPDEEKPSEAGCPEMEVPLIDL 60

Query: 61  SGFLSGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQS 120
           SGFLSG+KD+  EAVR VGEACEKHGFFLVVNHGVD KLI +AHKYM+EFFELPLC+KQ 
Sbjct: 61  SGFLSGDKDSVREAVRHVGEACEKHGFFLVVNHGVDLKLIADAHKYMNEFFELPLCEKQR 120

Query: 121 AQRKAGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGR 180
           AQRK GEHCGYASSFTGRFSSKLPWKETLSFRFSADES ++ V HYL  KLGD+FA FGR
Sbjct: 121 AQRKVGEHCGYASSFTGRFSSKLPWKETLSFRFSADESLDDHVFHYLRTKLGDRFANFGR 180

Query: 181 VYQEYCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG 240
           VYQEYCEAMSGLSLGIMELLGKSLGVE +YFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG
Sbjct: 181 VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG 240

Query: 241 PHCDPTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 283
           PHCDPTSLTILHQDQVGGLQVFVDNQWRLI PNFDAFVVNIGDTFM
Sbjct: 241 PHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFM 286

[Close]
BLAST of Cp4.1LG05g06970 vs. TrEMBL
Match: E4W529_9ROSI (Gibberellin 20-oxidase OS=Populus simonii PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 7.8e-122
Identity = 205/280 (73.21%), Postives = 233/280 (83.21%), Query Frame = 1 

Query: 9   ESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAA 68
           + + DG   VFDA VLRHQ NIP+QFIWPD EKP    PEL+VPL+DL  FLSG   AA 
Sbjct: 24  QCKDDGKSFVFDAQVLRHQTNIPQQFIWPDHEKPNINAPELQVPLVDLGDFLSGNPVAAV 83

Query: 69  EAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYA 128
           EA RLVGEAC+KHGFFLVVNHGVD+ LI  AH YMD FFELPL +KQ AQRK GE CGYA
Sbjct: 84  EASRLVGEACQKHGFFLVVNHGVDKTLIAHAHNYMDTFFELPLSEKQKAQRKIGESCGYA 143

Query: 129 SSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGL 188
           SSFTGRFSSKLPWKETLSFR++A+++ +  +  Y H+++G+ FA+FGRVYQ+YCEAMS L
Sbjct: 144 SSFTGRFSSKLPWKETLSFRYTAEKNSSKHIEEYFHNRMGEDFAEFGRVYQDYCEAMSTL 203

Query: 189 SLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH 248
           SLGIMELLG SLGV  +YF+ FF +NDSIMRLN+YPPCQKP LTLGTGPHCDPTSLTILH
Sbjct: 204 SLGIMELLGMSLGVSREYFREFFNENDSIMRLNYYPPCQKPDLTLGTGPHCDPTSLTILH 263

Query: 249 QDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMVTNQSI 289
           QDQVGGLQVFVDN+WR I+PNFDAFVVNIGDTFM  +  I
Sbjct: 264 QDQVGGLQVFVDNEWRSISPNFDAFVVNIGDTFMALSNGI 303

[Close]
BLAST of Cp4.1LG05g06970 vs. TrEMBL
Match: O04280_PHAVU (Gibberellin 20-oxidase OS=Phaseolus vulgaris GN=PHAVU_004G123600g PE=2 SV=2)

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-121
Identity = 205/274 (74.82%), Postives = 233/274 (85.04%), Query Frame = 1 

Query: 9   ESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAA 68
           +++ D APLVFDAS+LRHQ N+PKQFIWPD+EKP    PEL VPLIDL GFLSG+  A  
Sbjct: 22  DNKEDEAPLVFDASLLRHQLNLPKQFIWPDDEKPCMNVPELVVPLIDLRGFLSGDPVATM 81

Query: 69  EAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYA 128
           EA R+VGEAC+KHGFFLVVNHG+D  LI  AH YMD+FFE+PL QKQ AQRK GEHCGYA
Sbjct: 82  EAARMVGEACQKHGFFLVVNHGIDANLISHAHSYMDDFFEVPLTQKQRAQRKTGEHCGYA 141

Query: 129 SSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGL 188
           SSFTGRFSSKLPWKETLSF+FSA+E  + +V  YL + LG +F +FGRVYQ+YC+AMS L
Sbjct: 142 SSFTGRFSSKLPWKETLSFQFSAEEKSSTIVKDYLCNTLGQEFEQFGRVYQDYCDAMSNL 201

Query: 189 SLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH 248
           SLGIMELLG SLGV +  F+ FF++NDSIMRLN+YPPCQKP LTLGTGPHCDPTSLTILH
Sbjct: 202 SLGIMELLGMSLGVGKACFREFFEENDSIMRLNYYPPCQKPDLTLGTGPHCDPTSLTILH 261

Query: 249 QDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 283
           QDQVGGLQVFVDN+W  I PNF+AFVVNIGDTFM
Sbjct: 262 QDQVGGLQVFVDNEWHSINPNFNAFVVNIGDTFM 295

[Close]
BLAST of Cp4.1LG05g06970 vs. TrEMBL
Match: A0A067JHT4_JATCU (Gibberellin 20 oxidase OS=Jatropha curcas GN=GA20ox2 PE=2 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 2.3e-121
Identity = 205/267 (76.78%), Postives = 232/267 (86.89%), Query Frame = 1 

Query: 16  PLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAAEAVRLVG 75
           PLVFDASVLR++ N+P+QFIWPD EKP    PEL VP IDL GFLSG+  AA EA +LVG
Sbjct: 21  PLVFDASVLRYKSNVPQQFIWPDHEKPTANAPELPVPHIDLGGFLSGDPVAAMEASKLVG 80

Query: 76  EACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRF 135
           EAC+KHGFFLVVNHGV++KLI +AH+YMD FFELPLC+KQ AQRK GEHCGYASSFTGRF
Sbjct: 81  EACKKHGFFLVVNHGVNQKLIQDAHRYMDSFFELPLCEKQKAQRKIGEHCGYASSFTGRF 140

Query: 136 SSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIMEL 195
           SSKLPWKET+S R+SAD +   LV HYL + +G+ F++FGRVYQEYCEAMS LSLGIMEL
Sbjct: 141 SSKLPWKETVSVRYSADNNSPKLVQHYLRNTMGETFSEFGRVYQEYCEAMSTLSLGIMEL 200

Query: 196 LGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGL 255
           LG SLGV  ++F+ FF++NDSIMRLN+YP CQKP LTLGTGPHCDPTSLTILHQDQVGGL
Sbjct: 201 LGMSLGVSREHFREFFEENDSIMRLNYYPQCQKPDLTLGTGPHCDPTSLTILHQDQVGGL 260

Query: 256 QVFVDNQWRLITPNFDAFVVNIGDTFM 283
           QVFVDNQWR I+PNF AFVVNIGDTFM
Sbjct: 261 QVFVDNQWRSISPNFQAFVVNIGDTFM 287

[Close]
BLAST of Cp4.1LG05g06970 vs. TAIR10
Match: AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)

HSP 1 Score: 383.3 bits (983), Expect = 1.4e-106
Identity = 182/274 (66.42%), Postives = 209/274 (76.28%), Query Frame = 1 

Query: 15  APLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAAEAVRLV 74
           +PL+F+ S+L  Q  IP QFIWPDEEKP+   PEL VP IDLS      +D+  EA R++
Sbjct: 27  SPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDLSS-----QDSTLEAPRVI 86

Query: 75  GEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGR 134
            EAC KHGFFLVVNHGV   LI +AH+ M+ FF++PL  KQ AQRK GE CGYASSFTGR
Sbjct: 87  AEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGR 146

Query: 135 FSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIME 194
           FS+KLPWKETLSF+FS D S +  V  Y  D LG +F +FG+VYQ+YCEAMS LSL IME
Sbjct: 147 FSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIME 206

Query: 195 LLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGG 254
           LLG SLGV   YF+ FF++NDSIMRLN YPPCQ P LTLGTGPHCDP+SLTILHQD V G
Sbjct: 207 LLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNG 266

Query: 255 LQVFVDNQWRLITPNFDAFVVNIGDTFMVTNQSI 289
           LQVFVDNQW+ I PN  AFVVNIGDTFM  +  I
Sbjct: 267 LQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGI 295

[Close]
BLAST of Cp4.1LG05g06970 vs. TAIR10
Match: AT5G07200.1 (AT5G07200.1 gibberellin 20-oxidase 3)

HSP 1 Score: 374.8 bits (961), Expect = 5.0e-104
Identity = 179/275 (65.09%), Postives = 216/275 (78.55%), Query Frame = 1 

Query: 18  VFDASVL-RHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAAEAVRLVGE 77
           +FDA +L +H H+IP+QF+WPD EKP+T    L+VPLIDL+GFLSG+   A+EA RLV +
Sbjct: 24  IFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSK 83

Query: 78  ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
           A  KHGFFL+ NHGVD  L+  A+ +MD FF+ P C+KQ AQRK GE  GYASSF GRFS
Sbjct: 84  AATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFS 143

Query: 138 SKLPWKETLSFRFSADESFNN-LVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIMEL 197
           SKLPWKETLSF+FS +E  ++  V  ++  K+GD +  FG+VYQEY EAM+ LSL IMEL
Sbjct: 144 SKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMEL 203

Query: 198 LGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGL 257
           LG SLGVE +YFK FF+D+DSI RLN+YP C++P L LGTGPHCDPTSLTILHQDQVGGL
Sbjct: 204 LGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGL 263

Query: 258 QVFVDNQWRLITPNFDAFVVNIGDTFM-VTNQSIK 290
           QVFVDN+W+ I PN  AFVVNIGDTFM +TN   K
Sbjct: 264 QVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYK 298

[Close]
BLAST of Cp4.1LG05g06970 vs. TAIR10
Match: AT4G25420.1 (AT4G25420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 374.8 bits (961), Expect = 5.0e-104
Identity = 179/267 (67.04%), Postives = 207/267 (77.53%), Query Frame = 1 

Query: 16  PLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAAEAVRLVG 75
           PL+F+ S+L  Q NIP QFIWPD+EKP+    EL+VPLIDL   LS +  +  +A RL+ 
Sbjct: 26  PLIFNPSMLNLQANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLIS 85

Query: 76  EACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRF 135
           EAC+KHGFFLVVNHG+  +LI +AH+Y   FF++PL +KQ   RK+GE  GYASSFTGRF
Sbjct: 86  EACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRF 145

Query: 136 SSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIMEL 195
           S+KLPWKETLSFRF  D S +  V  Y  D LG  F  FG+VYQEYCEAMS LSL IMEL
Sbjct: 146 STKLPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMEL 205

Query: 196 LGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGL 255
           LG SLGV+  YF+ FF++NDSIMRLN+YPPC KP LTLGTGPHCDPTSLTILHQD V GL
Sbjct: 206 LGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGL 265

Query: 256 QVFVDNQWRLITPNFDAFVVNIGDTFM 283
           QVFV+NQWR I PN  AFVVNIGDTFM
Sbjct: 266 QVFVENQWRSIRPNPKAFVVNIGDTFM 291

[Close]
BLAST of Cp4.1LG05g06970 vs. TAIR10
Match: AT1G60980.1 (AT1G60980.1 gibberellin 20-oxidase 4)

HSP 1 Score: 342.4 bits (877), Expect = 2.8e-94
Identity = 164/275 (59.64%), Postives = 200/275 (72.73%), Query Frame = 1 

Query: 18  VFDASVLRHQ-HNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAAEAVRLVGE 77
           +FD++VL HQ  +IP++F+WPD EKP+   P L+VP+IDL+GFLS +    +EA RLV E
Sbjct: 23  IFDSTVLNHQPDHIPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSE 82

Query: 78  ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
           A +KHGFFLV NHGVD +L+  AHK MD FF+ P  +K  AQRK GE  GYASSF GRF 
Sbjct: 83  AAKKHGFFLVTNHGVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFK 142

Query: 138 SKLPWKETLSFRFSA---DESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGLSLGIM 197
             LPWKETLSF FS     E+++  V +Y+   +GD +  FG VYQEY E MS LSL IM
Sbjct: 143 ENLPWKETLSFSFSPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIM 202

Query: 198 ELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVG 257
           ELLG SLG++ ++F+ FF+DN+SI RLN+YP C++P L LGTGPHCDPTSLTIL QDQV 
Sbjct: 203 ELLGMSLGIKREHFREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVS 262

Query: 258 GLQVFVDNQWRLITPNFDAFVVNIGDTFMVTNQSI 289
           GLQVFVDNQW+ I P   A VVNIGDT M     I
Sbjct: 263 GLQVFVDNQWQSIPPIPQALVVNIGDTLMALTNGI 297

[Close]
BLAST of Cp4.1LG05g06970 vs. TAIR10
Match: AT1G44090.1 (AT1G44090.1 gibberellin 20-oxidase 5)

HSP 1 Score: 232.3 bits (591), Expect = 4.0e-61
Identity = 118/276 (42.75%), Postives = 174/276 (63.04%), Query Frame = 1 

Query: 8   PESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAA 67
           P+SR   +  V + S+L+ Q N+P +F WP+++  A +  +L++P+IDLSGFL+G +   
Sbjct: 22  PKSREMNSSDV-NFSLLQSQPNVPAEFFWPEKDV-APSEGDLDLPIIDLSGFLNGNEAET 81

Query: 68  AEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGY 127
             A + V +AC  HG FLVVNHG    L  +A +    FF L   +K  A R  G   GY
Sbjct: 82  QLAAKAVKKACMAHGTFLVVNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGY 141

Query: 128 ASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSG 187
            +  + RFSS LPW ETL+  F       ++V  +L  +LG+   + G+V+QE+C+AM+G
Sbjct: 142 TAGHSQRFSSNLPWNETLTLAFKKGPP--HVVEDFLTSRLGNHRQEIGQVFQEFCDAMNG 201

Query: 188 LSLGIMELLGKSLGVEEQ-YFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTI 247
           L + +MELLG S+G++++ Y++ FF+D   I R N+YPPC++P   LG GPH DPT++T+
Sbjct: 202 LVMDLMELLGISMGLKDRTYYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITV 261

Query: 248 LHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 283
           L QD V GL+VF    W+ + P   A VVN+GDTFM
Sbjct: 262 LLQDDVVGLEVFAAGSWQTVRPRPGALVVNVGDTFM 293

[Close]
BLAST of Cp4.1LG05g06970 vs. NCBI nr
Match: gi|27124556|emb|CAC83626.1| (gibberellin 20-oxidase [Cucurbita maxima])

HSP 1 Score: 578.9 bits (1491), Expect = 5.0e-162
Identity = 274/282 (97.16%), Postives = 278/282 (98.58%), Query Frame = 1 

Query: 1   MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFL 60
           MHVVTSTPE+RHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPA TCPELEVPLIDLSGFL
Sbjct: 1   MHVVTSTPEARHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELEVPLIDLSGFL 60

Query: 61  SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
           SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPL QKQSAQRK
Sbjct: 61  SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLSQKQSAQRK 120

Query: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQE 180
           AGEHCGYASSFTGRFSSKLPWKETLSFRF+ADES NNLVLHYL+DKLGDQFAKFGRVYQ+
Sbjct: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFAADESLNNLVLHYLNDKLGDQFAKFGRVYQD 180

Query: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
           YCEAMSGLSLGIMELLGKSLGVEEQ FKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD
Sbjct: 181 YCEAMSGLSLGIMELLGKSLGVEEQCFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240

Query: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 283
           PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM
Sbjct: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 282

[Close]
BLAST of Cp4.1LG05g06970 vs. NCBI nr
Match: gi|449451795|ref|XP_004143646.1| (PREDICTED: gibberellin 20 oxidase 1-D [Cucumis sativus])

HSP 1 Score: 517.3 bits (1331), Expect = 1.8e-143
Identity = 246/286 (86.01%), Postives = 260/286 (90.91%), Query Frame = 1 

Query: 1   MHVVTST---PESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATT-CPELEVPLIDL 60
           MHVVTS+     + +DG PLVFDASVLRHQH IPKQFIWPDEEKP+   CPE+EVPLIDL
Sbjct: 1   MHVVTSSMAKEAANNDGVPLVFDASVLRHQHKIPKQFIWPDEEKPSEAGCPEMEVPLIDL 60

Query: 61  SGFLSGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQS 120
           SGFLSG+KD+  EAVR VGEACEKHGFFLVVNHGVD KLI +AHKYM+EFFELPLC+KQ 
Sbjct: 61  SGFLSGDKDSVREAVRHVGEACEKHGFFLVVNHGVDLKLIADAHKYMNEFFELPLCEKQR 120

Query: 121 AQRKAGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGR 180
           AQRK GEHCGYASSFTGRFSSKLPWKETLSFRFSADES ++ V HYL  KLGD+FA FGR
Sbjct: 121 AQRKVGEHCGYASSFTGRFSSKLPWKETLSFRFSADESLDDHVFHYLRTKLGDRFANFGR 180

Query: 181 VYQEYCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG 240
           VYQEYCEAMSGLSLGIMELLGKSLGVE +YFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG
Sbjct: 181 VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG 240

Query: 241 PHCDPTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 283
           PHCDPTSLTILHQDQVGGLQVFVDNQWRLI PNFDAFVVNIGDTFM
Sbjct: 241 PHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFM 286

[Close]
BLAST of Cp4.1LG05g06970 vs. NCBI nr
Match: gi|659073104|ref|XP_008467255.1| (PREDICTED: gibberellin 20 oxidase 1-like [Cucumis melo])

HSP 1 Score: 501.9 bits (1291), Expect = 7.7e-139
Identity = 239/286 (83.57%), Postives = 256/286 (89.51%), Query Frame = 1 

Query: 1   MHVVTST---PESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATT-CPELEVPLIDL 60
           MHVVTS+     +++D  PLVFDAS LR+QH IPKQFIWPDEEKP+   CPE+EVPLIDL
Sbjct: 1   MHVVTSSMAKEAAKNDDVPLVFDASFLRYQHKIPKQFIWPDEEKPSEQQCPEMEVPLIDL 60

Query: 61  SGFLSGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQS 120
           SGFLSG+KD+  EAVR VGEACEKHGFFLVVNHGVD KLI +AHKYM++FFELPLC+KQ 
Sbjct: 61  SGFLSGDKDSVREAVRHVGEACEKHGFFLVVNHGVDLKLIADAHKYMNDFFELPLCEKQR 120

Query: 121 AQRKAGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGR 180
           AQRK GEHCGYASSFTGRFSSKLPWKETLSFRFSADES ++ V HYL  KLGD+F  FGR
Sbjct: 121 AQRKVGEHCGYASSFTGRFSSKLPWKETLSFRFSADESLDDHVFHYLRTKLGDRFVNFGR 180

Query: 181 VYQEYCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG 240
           VYQEYCEAMSGLSLGIMELLGKSLGV  +YFKNFFKDNDSIMRLNFYPPCQKP LTLGTG
Sbjct: 181 VYQEYCEAMSGLSLGIMELLGKSLGVGGEYFKNFFKDNDSIMRLNFYPPCQKPDLTLGTG 240

Query: 241 PHCDPTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 283
           PHCDPTSLTILHQDQVGGLQVFVDNQWRLI PNFDAFVVNIGDTFM
Sbjct: 241 PHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFM 286

[Close]
BLAST of Cp4.1LG05g06970 vs. NCBI nr
Match: gi|233142142|gb|ACQ91097.1| (gibberellin 20-oxidase [Populus simonii])

HSP 1 Score: 444.9 bits (1143), Expect = 1.1e-121
Identity = 205/280 (73.21%), Postives = 233/280 (83.21%), Query Frame = 1 

Query: 9   ESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAA 68
           + + DG   VFDA VLRHQ NIP+QFIWPD EKP    PEL+VPL+DL  FLSG   AA 
Sbjct: 24  QCKDDGKSFVFDAQVLRHQTNIPQQFIWPDHEKPNINAPELQVPLVDLGDFLSGNPVAAV 83

Query: 69  EAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYA 128
           EA RLVGEAC+KHGFFLVVNHGVD+ LI  AH YMD FFELPL +KQ AQRK GE CGYA
Sbjct: 84  EASRLVGEACQKHGFFLVVNHGVDKTLIAHAHNYMDTFFELPLSEKQKAQRKIGESCGYA 143

Query: 129 SSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGL 188
           SSFTGRFSSKLPWKETLSFR++A+++ +  +  Y H+++G+ FA+FGRVYQ+YCEAMS L
Sbjct: 144 SSFTGRFSSKLPWKETLSFRYTAEKNSSKHIEEYFHNRMGEDFAEFGRVYQDYCEAMSTL 203

Query: 189 SLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH 248
           SLGIMELLG SLGV  +YF+ FF +NDSIMRLN+YPPCQKP LTLGTGPHCDPTSLTILH
Sbjct: 204 SLGIMELLGMSLGVSREYFREFFNENDSIMRLNYYPPCQKPDLTLGTGPHCDPTSLTILH 263

Query: 249 QDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMVTNQSI 289
           QDQVGGLQVFVDN+WR I+PNFDAFVVNIGDTFM  +  I
Sbjct: 264 QDQVGGLQVFVDNEWRSISPNFDAFVVNIGDTFMALSNGI 303

[Close]
BLAST of Cp4.1LG05g06970 vs. NCBI nr
Match: gi|593703919|ref|XP_007152362.1| (hypothetical protein PHAVU_004G123600g [Phaseolus vulgaris])

HSP 1 Score: 443.7 bits (1140), Expect = 2.5e-121
Identity = 205/274 (74.82%), Postives = 233/274 (85.04%), Query Frame = 1 

Query: 9   ESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFLSGEKDAAA 68
           +++ D APLVFDAS+LRHQ N+PKQFIWPD+EKP    PEL VPLIDL GFLSG+  A  
Sbjct: 22  DNKEDEAPLVFDASLLRHQLNLPKQFIWPDDEKPCMNVPELVVPLIDLRGFLSGDPVATM 81

Query: 69  EAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYA 128
           EA R+VGEAC+KHGFFLVVNHG+D  LI  AH YMD+FFE+PL QKQ AQRK GEHCGYA
Sbjct: 82  EAARMVGEACQKHGFFLVVNHGIDANLISHAHSYMDDFFEVPLTQKQRAQRKTGEHCGYA 141

Query: 129 SSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQEYCEAMSGL 188
           SSFTGRFSSKLPWKETLSF+FSA+E  + +V  YL + LG +F +FGRVYQ+YC+AMS L
Sbjct: 142 SSFTGRFSSKLPWKETLSFQFSAEEKSSTIVKDYLCNTLGQEFEQFGRVYQDYCDAMSNL 201

Query: 189 SLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH 248
           SLGIMELLG SLGV +  F+ FF++NDSIMRLN+YPPCQKP LTLGTGPHCDPTSLTILH
Sbjct: 202 SLGIMELLGMSLGVGKACFREFFEENDSIMRLNYYPPCQKPDLTLGTGPHCDPTSLTILH 261

Query: 249 QDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFM 283
           QDQVGGLQVFVDN+W  I PNF+AFVVNIGDTFM
Sbjct: 262 QDQVGGLQVFVDNEWHSINPNFNAFVVNIGDTFM 295

The following BLAST results are available for this feature:
BLAST of Cp4.1LG05g06970 vs. Swiss-Prot
Match NameE-valueIdentityDescription
GAOX2_ARATH2.5e-10566.42Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1[more]
GAOX3_ARATH8.9e-10365.09Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1[more]
GAOX1_ARATH8.9e-10367.04Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2[more]
GAO1D_WHEAT1.7e-9362.41Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1[more]
GAO1A_WHEAT2.2e-9362.03Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1[more]
BLAST of Cp4.1LG05g06970 vs. TrEMBL
Match NameE-valueIdentityDescription
Q8GT56_CUCMA3.5e-16297.16Gibberellin 20-oxidase OS=Cucurbita maxima GN=ga20ox PE=2 SV=1[more]
K7ZU06_CUCSA1.2e-14386.01Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus GN=ga20ox1 PE=2 SV=1[more]
E4W529_9ROSI7.8e-12273.21Gibberellin 20-oxidase OS=Populus simonii PE=2 SV=1[more]
O04280_PHAVU1.7e-12174.82Gibberellin 20-oxidase OS=Phaseolus vulgaris GN=PHAVU_004G123600g PE=2 SV=2[more]
A0A067JHT4_JATCU2.3e-12176.78Gibberellin 20 oxidase OS=Jatropha curcas GN=GA20ox2 PE=2 SV=1[more]
BLAST of Cp4.1LG05g06970 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G51810.11.4e-10666.42 gibberellin 20 oxidase 2[more]
AT5G07200.15.0e-10465.09 gibberellin 20-oxidase 3[more]
AT4G25420.15.0e-10467.04 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G60980.12.8e-9459.64 gibberellin 20-oxidase 4[more]
AT1G44090.14.0e-6142.75 gibberellin 20-oxidase 5[more]
BLAST of Cp4.1LG05g06970 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|27124556|emb|CAC83626.1|5.0e-16297.16gibberellin 20-oxidase [Cucurbita maxima][more]
gi|449451795|ref|XP_004143646.1|1.8e-14386.01PREDICTED: gibberellin 20 oxidase 1-D [Cucumis sativus][more]
gi|659073104|ref|XP_008467255.1|7.7e-13983.57PREDICTED: gibberellin 20 oxidase 1-like [Cucumis melo][more]
gi|233142142|gb|ACQ91097.1|1.1e-12173.21gibberellin 20-oxidase [Populus simonii][more]
gi|593703919|ref|XP_007152362.1|2.5e-12174.82hypothetical protein PHAVU_004G123600g [Phaseolus vulgaris][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0005506iron ion binding
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR002283Isopenicillin N synthase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0000105 histidine biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016707 gibberellin 3-beta-dioxygenase activity
molecular_function GO:0047927 gibberellin-44 dioxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004399 histidinol dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g06970.1Cp4.1LG05g06970.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002283Isopenicillin N synthasePRINTSPR00682IPNSYNTHASEcoord: 74..91
score: 3.4E-6coord: 238..259
score: 3.
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 215..286
score: 2.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 213..300
score: 9
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 51..151
score: 6.6
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 25..291
score: 1.9
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 4..283
score: 2.0E
NoneNo IPR availablePANTHERPTHR10209:SF140GIBBERELLIN 20 OXIDASE 1-RELATEDcoord: 4..283
score: 2.0E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 23..283
score: 1.99

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG05g06970Cucsa.115530Cucumber (Gy14) v1cgycpeB0272
Cp4.1LG05g06970CmaCh15G013930Cucurbita maxima (Rimu)cmacpeB331
Cp4.1LG05g06970CmaCh02G010210Cucurbita maxima (Rimu)cmacpeB646
Cp4.1LG05g06970CmoCh02G010360Cucurbita moschata (Rifu)cmocpeB595
Cp4.1LG05g06970CmoCh15G014590Cucurbita moschata (Rifu)cmocpeB295
Cp4.1LG05g06970Cla002362Watermelon (97103) v1cpewmB718
Cp4.1LG05g06970Csa5G172270Cucumber (Chinese Long) v2cpecuB737
Cp4.1LG05g06970MELO3C005397Melon (DHL92) v3.5.1cpemeB648
Cp4.1LG05g06970ClCG01G005290Watermelon (Charleston Gray)cpewcgB640
Cp4.1LG05g06970CSPI05G08170Wild cucumber (PI 183967)cpecpiB739
Cp4.1LG05g06970Lsi09G005620Bottle gourd (USVL1VR-Ls)cpelsiB583
Cp4.1LG05g06970MELO3C005397.2Melon (DHL92) v3.6.1cpemedB775
Cp4.1LG05g06970MELO3C006857.2Melon (DHL92) v3.6.1cpemedB835
Cp4.1LG05g06970CsaV3_5G005560Cucumber (Chinese Long) v3cpecucB0916
Cp4.1LG05g06970CsaV3_3G016600Cucumber (Chinese Long) v3cpecucB0903
Cp4.1LG05g06970Bhi01G001020Wax gourdcpewgoB0925
Cp4.1LG05g06970Bhi12G000637Wax gourdcpewgoB0919
Cp4.1LG05g06970CsGy5G005550Cucumber (Gy14) v2cgybcpeB692
Cp4.1LG05g06970Carg08456Silver-seed gourdcarcpeB1002
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG05g06970Cp4.1LG13g00530Cucurbita pepo (Zucchini)cpecpeB222
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG05g06970Cucurbita pepo (Zucchini)cpecpeB057
Cp4.1LG05g06970Cucurbita pepo (Zucchini)cpecpeB245
Cp4.1LG05g06970Cucumber (Gy14) v1cgycpeB0684
Cp4.1LG05g06970Cucurbita maxima (Rimu)cmacpeB358
Cp4.1LG05g06970Cucurbita maxima (Rimu)cmacpeB453
Cp4.1LG05g06970Cucurbita moschata (Rifu)cmocpeB322
Cp4.1LG05g06970Cucurbita moschata (Rifu)cmocpeB414
Cp4.1LG05g06970Wild cucumber (PI 183967)cpecpiB725
Cp4.1LG05g06970Cucumber (Chinese Long) v2cpecuB725
Cp4.1LG05g06970Watermelon (Charleston Gray)cpewcgB678
Cp4.1LG05g06970Watermelon (97103) v1cpewmB705
Cp4.1LG05g06970Watermelon (97103) v1cpewmB710
Cp4.1LG05g06970Melon (DHL92) v3.5.1cpemeB701
Cp4.1LG05g06970Silver-seed gourdcarcpeB1041
Cp4.1LG05g06970Wax gourdcpewgoB0914