Cp4.1LG05g04270 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG05g04270
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionFatty acyl-CoA reductase
LocationCp4.1LG05 : 2350617 .. 2352956 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAATCGAGAAAATTCTTCGGATTCAACCAAATGTGAAGAAACTCTACCTGTTATTAAGAGCAGCTGATGAAACTGAAGCTAAAAAACGTTTCTATAATGAGGTATAGATCCTAACAGGGACAGTTTTTGCATGTTTTTTTTGTTGTTGAACACGACAAACCATTGATTTTGCAGGTGATTGAGAAGGATTTGTTCCAAGTTTTGAGGAAGAAATGGGGTGCAAATCTAAATACCTTAATTTCCGAGAAGATTTGTTTGGTGCCGGGAGAGATCTCGCTCTCGCAAATGGGATTGAAAGATTCCATTTGGGTCGACGAGATGAAGAATCAAGTGGAAATAGTTATAAATTTAGCTGCAACTACTCATTTCGATGAAAGGTATGAACCCGTGTTGTGTCATAAATGGTTTCAATGTCTCGAACAACGTTGTTGTATGTTTGTGTTTGATAGGTACGATGTAGCACTTGACACCAATACGATTGGAGTTGAACATGTCGTAAGGTTTGCAAAGGAATGTCGTAATTTGAAGCTTCTTGTTCATGTATCTACTGGTTAGTATCTCGAACGATTTTTAGTTTCGACATTTATTTTAGATATTCAATAATGGAAGAAAATTTGTGGTTTTTAAGCTTATGTTTCGGGAGAAAGGGACGGAGTCATCTTGGAAAGTTCATATAAAATGGGTGAGTCTCTTAATGGCGTCAAAGGGCTCGACATTGAAGAAGAACAAGCCATTGTAGCGGACAAATTAAAACAGCTTAAAGAGAACGGCGCTACAGAAGAGTTCGTAACTCAAACTATGAAAGACTTAGGCCTTGAAAGGTAACATTCGTTATAAGGGTGTGAAAATCTCTATCTAACGGACGTATTTTAAAATTTTTGAGGGGAAGCTAACATATTGCTAGTGATGGACTCGGGCCGTTACACTGTAATAACTAGTTTTTTGTTTTGTTTTATTTACGATCAGGGCAAATCTATATGGATGGCCGAACACATACGTATTCACAAAGGCAATGGGTGAAATGACGATTGGTGACCTAAAAAACGATCTACCTCTGATCATCATTCGACCAACAATAGTTACGAGTACTTACAAAGAACCGTTTCCGGGTTGGGTCGAGGGAGTTAGGTACACGTTTTTAAAATACGTCTTAAATTGTAGTAGGTGGGGAGCATTAAAACAACCACAATATTGCCATATTTTGATAGGACCATTGATAGCATTATTGTTGGATATGCCAAAGGGAAGCTAACATGCTTTGCTGCTGGTACCAATTCAAGCCTTGATTTGGTAAGATGAGTATGCTCTTACATTTTTTCTAATACCCATTTGAATGCAAGGCACGAGATTGATCGAGTTTAGAAAAATGCAGATTCCGGCCGATATAGTGGTAAACACAATGATCATGGCGATGGCAGCTCATGAAGACCAGCCGTCTGATCAAACGATCTATCACGTGGGTTCTTCAGCGAGAAATTCCATGACGCATATTGATCTTCAACGTTTTAGTTATCAATATTTCACTCAGAAACCGTTGATCGACAAAAATGGAAAAGCCATTAAACTCGGAAAAGTTACCCTCTTCAACAGTATGGCTAAGTTTCATAGATACCTAGCCATTCGCTACTTAATTTTTCTCAAGGTACGTTCTTACCAATACAAACTCCATAAGATGGAAAATATAATCTCAATCCTCGGCCACATGGAGCTCACGGGTAAAAACTTCTCTGTGAGATCCTACATCGATTAGAGAGGGGAACGAAACATTTCTTATAAGGGTGTGGAAACCTCTCTATAGCAGACACATTTTAAAACTGTGAGTCTGACGACTATTTGAACAGGCCAAAACAGACAATATTTATTAGCAGTGGACTTAATTATGTAGGGACTCGAATTTGCAAACATAGCATTTTGCCATTCTTTTCAAGACAAATACGTCGATATGAGGAGGAAGTTCAATTTGGTGATGCGTCTCTTCGAACTTTATCGCCCCTATCTCTTCTTCAATGCTATGTAAGTATATGTTTCCGCGTTTAAATAATATTAAATTTTAGAGGTCAAATGAAAATATATGAACGTATAAGGATGAAAATGTAACAATGTGAAAGATTAATGCTTGTTTGTTTTCGTATGTACAGATTTGATGATACGAACACAGAGAGATTAAGGAATGATATACGAAACAAAGATACAAACGAAGCATTTGTTATGGATCCTAAAGATATCAACTGGGAAGATTACTTTATGAACATTCACTTTCCGGGACTTGTTAAACATGTTATCAGATAATCGTAATTTTATCTGGAAAAATAAAATTACTTATTTTTATCGATCAATTA

mRNA sequence

TTAATCGAGAAAATTCTTCGGATTCAACCAAATGTGAAGAAACTCTACCTGTTATTAAGAGCAGCTGATGAAACTGAAGCTAAAAAACGTTTCTATAATGAGGTGATTGAGAAGGATTTGTTCCAAGTTTTGAGGAAGAAATGGGGTGCAAATCTAAATACCTTAATTTCCGAGAAGATTTGTTTGGTGCCGGGAGAGATCTCGCTCTCGCAAATGGGATTGAAAGATTCCATTTGGGTCGACGAGATGAAGAATCAAGTGGAAATAGTTATAAATTTAGCTGCAACTACTCATTTCGATGAAAGGTACGATGTAGCACTTGACACCAATACGATTGGAGTTGAACATGTCGTAAGGTTTGCAAAGGAATGTCGTAATTTGAAGCTTCTTGTTCATGTATCTACTGCTTATGTTTCGGGAGAAAGGGACGGAGTCATCTTGGAAAGTTCATATAAAATGGGTGAGTCTCTTAATGGCGTCAAAGGGCTCGACATTGAAGAAGAACAAGCCATTGCAATGGGTGAAATGACGATTGGTGACCTAAAAAACGATCTACCTCTGATCATCATTCGACCAACAATAGTTACGAGTACTTACAAAGAACCGTTTCCGGGTTGGGTCGAGGGAGTTAGGACCATTGATAGCATTATTGTTGGATATGCCAAAGGGAAGCTAACATGCTTTGCTGCTGGTACCAATTCAAGCCTTGATTTGATTCCGGCCGATATAGTGGTAAACACAATGATCATGGCGATGGCAGCTCATGAAGACCAGCCGTCTGATCAAACGATCTATCACGTGGGTTCTTCAGCGAGAAATTCCATGACGCATATTGATCTTCAACGTTTTAGTTATCAATATTTCACTCAGAAACCGTTGATCGACAAAAATGGAAAAGCCATTAAACTCGGAAAAGTTACCCTCTTCAACAGTATGGCTAAATTTGATGATACGAACACAGAGAGATTAAGGAATGATATACGAAACAAAGATACAAACGAAGCATTTGTTATGGATCCTAAAGATATCAACTGGGAAGATTACTTTATGAACATTCACTTTCCGGGACTTGTTAAACATGTTATCAGATAATCGTAATTTTATCTGGAAAAATAAAATTACTTATTTTTATCGATCAATTA

Coding sequence (CDS)

TTAATCGAGAAAATTCTTCGGATTCAACCAAATGTGAAGAAACTCTACCTGTTATTAAGAGCAGCTGATGAAACTGAAGCTAAAAAACGTTTCTATAATGAGGTGATTGAGAAGGATTTGTTCCAAGTTTTGAGGAAGAAATGGGGTGCAAATCTAAATACCTTAATTTCCGAGAAGATTTGTTTGGTGCCGGGAGAGATCTCGCTCTCGCAAATGGGATTGAAAGATTCCATTTGGGTCGACGAGATGAAGAATCAAGTGGAAATAGTTATAAATTTAGCTGCAACTACTCATTTCGATGAAAGGTACGATGTAGCACTTGACACCAATACGATTGGAGTTGAACATGTCGTAAGGTTTGCAAAGGAATGTCGTAATTTGAAGCTTCTTGTTCATGTATCTACTGCTTATGTTTCGGGAGAAAGGGACGGAGTCATCTTGGAAAGTTCATATAAAATGGGTGAGTCTCTTAATGGCGTCAAAGGGCTCGACATTGAAGAAGAACAAGCCATTGCAATGGGTGAAATGACGATTGGTGACCTAAAAAACGATCTACCTCTGATCATCATTCGACCAACAATAGTTACGAGTACTTACAAAGAACCGTTTCCGGGTTGGGTCGAGGGAGTTAGGACCATTGATAGCATTATTGTTGGATATGCCAAAGGGAAGCTAACATGCTTTGCTGCTGGTACCAATTCAAGCCTTGATTTGATTCCGGCCGATATAGTGGTAAACACAATGATCATGGCGATGGCAGCTCATGAAGACCAGCCGTCTGATCAAACGATCTATCACGTGGGTTCTTCAGCGAGAAATTCCATGACGCATATTGATCTTCAACGTTTTAGTTATCAATATTTCACTCAGAAACCGTTGATCGACAAAAATGGAAAAGCCATTAAACTCGGAAAAGTTACCCTCTTCAACAGTATGGCTAAATTTGATGATACGAACACAGAGAGATTAAGGAATGATATACGAAACAAAGATACAAACGAAGCATTTGTTATGGATCCTAAAGATATCAACTGGGAAGATTACTTTATGAACATTCACTTTCCGGGACTTGTTAAACATGTTATCAGATAA

Protein sequence

LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKICLVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRFAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKGLDIEEEQAIAMGEMTIGDLKNDLPLIIIRPTIVTSTYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHEDQPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLFNSMAKFDDTNTERLRNDIRNKDTNEAFVMDPKDINWEDYFMNIHFPGLVKHVIR
BLAST of Cp4.1LG05g04270 vs. Swiss-Prot
Match: FACR4_ARATH (Probable fatty acyl-CoA reductase 4 OS=Arabidopsis thaliana GN=FAR4 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.6e-84
Identity = 178/465 (38.28%), Postives = 257/465 (55.27%), Query Frame = 1

Query: 2   IEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWG-ANLNTLISEKI 61
           +EKILRIQP VKKL+LLLRAAD   A +RF++EV+EKDLF+VL+   G  NL   I+EK+
Sbjct: 28  VEKILRIQPKVKKLFLLLRAADNESAMQRFHSEVLEKDLFRVLKNALGDENLKAFITEKV 87

Query: 62  CLVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRF 121
             +PG+IS+  +G+K S  +  M N+++I++N+AATT+FDERYDV L  NT G  +V+ F
Sbjct: 88  VPIPGDISVDNLGVKGSDLLQHMWNEIDIIVNVAATTNFDERYDVGLSVNTFGPLNVLNF 147

Query: 122 AKECRNLKLLVHVSTAYVSGERDGV------------------ILESSYK-MGESLNGVK 181
           AK+C   +LL+HVSTAYV GE+ G+                  ++E+  + M + L  ++
Sbjct: 148 AKKCVKGQLLLHVSTAYVRGEKSGLLHEKTFHMGETLNGHRKLVIETEMELMKQKLKELQ 207

Query: 182 GLDIEEEQAI------------------------AMGEMTIGDLKNDLPLIIIRPTIVTS 241
             +  EE+                          +MGEM +G+ + +LP++IIRPT++TS
Sbjct: 208 KQNCSEEEISQSMKDLGMSRAKLHGWPNTYVFTKSMGEMLLGNYRENLPIVIIRPTMITS 267

Query: 242 TYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHED 301
           T+ EPFPGW+EG+RTIDS+IV Y KG+L CF A  NS LDLIP D+V N M+ A A H  
Sbjct: 268 TFSEPFPGWIEGLRTIDSVIVAYGKGRLKCFLADPNSVLDLIPVDMVANAMVTAAAIHAG 327

Query: 302 QPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLFNSMAKFDD 361
           +   QT+YHVGSS +N +T   +   +  YFT+ PL+ ++G +I + K T+ ++MA+F  
Sbjct: 328 KLGSQTVYHVGSSCKNPITFEQIHDLAASYFTKNPLVRRDGSSILVSKGTILSTMAQFSF 387

Query: 362 TNTERLR----------------NDIRNKDTNE--------------------------- 364
             T R +                N  + KD +                            
Sbjct: 388 YMTLRYKLPLQMLRLIYVIYPWWNGNKYKDIDRKIKLAMRLVDLYRPYVLFKGIFDDTNT 447

BLAST of Cp4.1LG05g04270 vs. Swiss-Prot
Match: FACR2_ARATH (Fatty acyl-CoA reductase 2 OS=Arabidopsis thaliana GN=FAR2 PE=2 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 7.8e-61
Identity = 136/368 (36.96%), Postives = 205/368 (55.71%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKI 60
           LIEK+LR+ P+V K+YLL++A  +  A +R  NEV++ +LF  L++  GA+  + +  K+
Sbjct: 146 LIEKVLRMAPDVSKIYLLIKAKSKEAAIERLKNEVLDAELFNTLKETHGASYMSFMLTKL 205

Query: 61  CLVPGEISLSQMGLK-DSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVR 120
             V G I  S +GL+ DS   +E+  +V+++IN AA T F+ERYDVALD NT G  +++ 
Sbjct: 206 IPVTGNICDSNIGLQADS--AEEIAKEVDVIINSAANTTFNERYDVALDINTRGPGNLMG 265

Query: 121 FAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGE------------------------ 180
           FAK+C+ LKL + VSTAYV+G+R G I+E  + MG+                        
Sbjct: 266 FAKKCKKLKLFLQVSTAYVNGQRQGRIMEKPFSMGDCIATENFLEGNRKALDVDREMKLA 325

Query: 181 ------------SLNGVKGLDIEEEQAI----------AMGEMTIGDLKNDLPLIIIRPT 240
                           +K L +E  ++           AMGEM I   + D+P++IIRP+
Sbjct: 326 LEAARKGTQNQDEAQKMKDLGLERARSYGWQDTYVFTKAMGEMMINSTRGDVPVVIIRPS 385

Query: 241 IVTSTYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMA 300
           ++ STYK+PFPGW+EG R +D I++ Y KG+LT F       LD++PAD+VVN  + A+A
Sbjct: 386 VIESTYKDPFPGWMEGNRMMDPIVLCYGKGQLTGFLVDPKGVLDVVPADMVVNATLAAIA 445

Query: 301 AH----EDQPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLF 318
            H     D   +  +Y + SSA N +   DL    Y ++   P +D  G  I +  + LF
Sbjct: 446 KHGMAMSDPEPEINVYQIASSAINPLVFEDLAELLYNHYKTSPCMDSKGDPIMVRLMKLF 505

BLAST of Cp4.1LG05g04270 vs. Swiss-Prot
Match: FACR3_ARATH (Fatty acyl-CoA reductase 3 OS=Arabidopsis thaliana GN=FAR3 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-51
Identity = 114/252 (45.24%), Postives = 161/252 (63.89%), Query Frame = 1

Query: 2   IEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKIC 61
           +EKILR+ PNVKKLYLLLRA+    A +RF +E+++KDLF+VL++K+G NLN L SEKI 
Sbjct: 33  VEKILRVAPNVKKLYLLLRASKGKSATQRFNDEILKKDLFKVLKEKYGPNLNQLTSEKIT 92

Query: 62  LVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRFA 121
           +V G+I L  +GL+D     EM +QV+ ++NLAATT FDERYDVAL  NT+G  +V+ FA
Sbjct: 93  IVDGDICLEDLGLQDFDLAHEMIHQVDAIVNLAATTKFDERYDVALGINTLGALNVLNFA 152

Query: 122 KECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKGLDIEEEQAIAMGEMTIGDL 181
           K C  +K+LVHVSTAYV GE+ G+I+E+ Y+MGE+LNG  GLDI  E+ +   ++     
Sbjct: 153 KRCAKVKILVHVSTAYVCGEKSGLIMETPYRMGETLNGTTGLDINYEKKLVQEKL----- 212

Query: 182 KNDLPLIIIRPTIVTSTYKE------PFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSS 241
            + L +I   P  +T T K+         GW       ++ +   A G++       N S
Sbjct: 213 -DQLRVIGAAPETITETMKDLGLRRAKMYGWP------NTYVFTKAMGEMMVGTKRENLS 272

Query: 242 LDLIPADIVVNT 248
           L L+   I+ +T
Sbjct: 273 LVLLRPSIITST 272

BLAST of Cp4.1LG05g04270 vs. Swiss-Prot
Match: FACR7_ARATH (Putative fatty acyl-CoA reductase 7 OS=Arabidopsis thaliana GN=FAR7 PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-47
Identity = 104/259 (40.15%), Postives = 146/259 (56.37%), Query Frame = 1

Query: 100 DERYDVALDTNTIGVEHVVRFAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNG 159
           ++RYDVAL  NT G  +V+ FAK+C   KLL+HVST YV GER G I+E  + MGESLNG
Sbjct: 54  EQRYDVALGINTFGAINVLNFAKKCVKPKLLLHVSTVYVCGERPGHIVEKHFAMGESLNG 113

Query: 160 VKGL----------------------DIEEEQAI---------------------AMGEM 219
              +                      + E EQA+                     AMGEM
Sbjct: 114 KNKVDINTERRLADQKSKQFKEQGCSEEETEQAMKDFGLKRARLYGWPNTYVFTKAMGEM 173

Query: 220 TIGDLKNDLPLIIIRPTIVTSTYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSL 279
            +G  +  +P++IIRPTI+TST+ +PFPGW+EG++T+DS+I+ Y KG L CF     +  
Sbjct: 174 LLGHYRETMPIVIIRPTIITSTFSDPFPGWIEGLKTVDSVIIFYGKGILKCFLVDQKTVC 233

Query: 280 DLIPADIVVNTMIMAMAAHEDQPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDK 316
           D+IP D+VVN MI   A H       T+YHVGSS +N + +  +     +YF + PL+ +
Sbjct: 234 DIIPVDMVVNAMIAIAADHCHDSGSHTVYHVGSSNQNPVIYKQIYEMMSRYFMKSPLVGR 293

BLAST of Cp4.1LG05g04270 vs. Swiss-Prot
Match: FACR1_ARATH (Fatty acyl-CoA reductase 1 OS=Arabidopsis thaliana GN=FAR1 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.1e-46
Identity = 95/169 (56.21%), Postives = 129/169 (76.33%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGA-NLNTLISEK 60
           L+EKILR+QPNVKK+YLLLRA DE  A +R  +EV+E DLF+VLR   G  NLN L+ EK
Sbjct: 27  LVEKILRLQPNVKKIYLLLRAPDEKSAMQRLRSEVMEIDLFKVLRNNLGEDNLNALMREK 86

Query: 61  ICLVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVR 120
           I  VPG+IS+  +GLKD+  +  M ++++I+IN+AATT+FDERYD+ L  NT G  +V+ 
Sbjct: 87  IVPVPGDISIDNLGLKDTDLIQRMWSEIDIIINIAATTNFDERYDIGLGINTFGALNVLN 146

Query: 121 FAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKGLDIEEE 169
           FAK+C   +LL+HVSTAY+SGE+ G++LE  +KMGE+L+G + LDI  E
Sbjct: 147 FAKKCVKGQLLLHVSTAYISGEQPGLLLEKPFKMGETLSGDRELDINIE 195

BLAST of Cp4.1LG05g04270 vs. TrEMBL
Match: A0A0A0KDN1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G151810 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.7e-123
Identity = 237/371 (63.88%), Postives = 278/371 (74.93%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKI 60
           L+EKILR+QPNVKKLYLLLRA DE  AKKRF+NEV+EK LFQVL+KK GANLNTLISEKI
Sbjct: 22  LVEKILRVQPNVKKLYLLLRADDEITAKKRFHNEVVEKALFQVLKKKHGANLNTLISEKI 81

Query: 61  CLVPGEISLSQMGLK-DSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVR 120
           CLVPGEISL QMGLK DSIW+D++K+QVEI+INLAATT+FDERYDVAL TN +G +HV+ 
Sbjct: 82  CLVPGEISLPQMGLKHDSIWIDKLKSQVEIIINLAATTNFDERYDVALGTNALGAKHVIN 141

Query: 121 FAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLN--------------------- 180
           FAK+C NLKL+VHVSTAYVSGER+G+I+E+ YKMGESLN                     
Sbjct: 142 FAKQCSNLKLVVHVSTAYVSGEREGLIMETPYKMGESLNWFKGLDIEVEQMVIEDKLKQL 201

Query: 181 ------------GVKGLDIEEEQAI----------AMGEMTIGDLKNDLPLIIIRPTIVT 240
                        +K L +E  +            AMGEM IGDLK+++PLI+IRPTIVT
Sbjct: 202 KEDGATNETITLAMKDLGLERSKLYGWPNTYVFTKAMGEMIIGDLKDEVPLIVIRPTIVT 261

Query: 241 STYKEPFPGWVEGVR------------TIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIV 300
           STYKEPFPGW+EGVR            TIDS+IVGYAKGKLTCF AG NS +DLIPAD+V
Sbjct: 262 STYKEPFPGWIEGVRIWGQTLCINLCRTIDSLIVGYAKGKLTCFVAGINSIIDLIPADMV 321

Query: 301 VNTMIMAMAAHEDQPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLG 316
           VNT+IMAM  H+ QPS+  IYHVGSS RN + H D QRF+YQYFT+KPLID++G AIK+G
Sbjct: 322 VNTIIMAMMVHKLQPSNHIIYHVGSSKRNPIKHADFQRFNYQYFTKKPLIDRDGNAIKVG 381

BLAST of Cp4.1LG05g04270 vs. TrEMBL
Match: A0A0A0KB44_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G152310 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.0e-108
Identity = 211/358 (58.94%), Postives = 260/358 (72.63%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKI 60
           L+EKILRIQPNVKKLYLLLRA DET A +RF NEV+ KDLF+VL++KWGANL++ ISEK+
Sbjct: 30  LLEKILRIQPNVKKLYLLLRATDETTAMQRFCNEVMGKDLFKVLKEKWGANLDSFISEKV 89

Query: 61  CLVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRF 120
            +V  +IS+S MGLKDSI V+E+K+ V+I+INLAATT+F+ERYDVAL TNT+G +HVV F
Sbjct: 90  SVVSSDISVSDMGLKDSILVEEIKHNVQIIINLAATTNFNERYDVALGTNTLGAKHVVDF 149

Query: 121 AKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKGLDIEEEQ----------- 180
           AK+C N KLLVHVSTAY+SGE++G ILE+ +K+GESLNG +GL+IE EQ           
Sbjct: 150 AKQCPNFKLLVHVSTAYISGEKEGFILETPHKLGESLNGTEGLNIEIEQKIIEQRLKQLK 209

Query: 181 --------------------------------AIAMGEMTIGDLKNDLPLIIIRPTIVTS 240
                                             AMGEM I DLK++LPLIIIRPTI+TS
Sbjct: 210 DNGASEKDVTIAMKDLGIQRANQYGWPNTYVFTKAMGEMLINDLKHNLPLIIIRPTIITS 269

Query: 241 TYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHED 300
           TYKEPF GW+EG RTIDS+IVGYAKGKLT     TNS +D+IPAD+VVNT+I  M  H+ 
Sbjct: 270 TYKEPFSGWIEGTRTIDSLIVGYAKGKLTFIPCDTNSIIDVIPADMVVNTIIATMVMHKL 329

Query: 301 QPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLFNSMAKF 316
           Q     IYHVGSS RN M   DLQRF  +YFT+KP I+ +G AIK+ K+T+FN++A F
Sbjct: 330 QYRQTIIYHVGSSTRNPMKIDDLQRFICKYFTEKPWINGDGNAIKVKKITVFNNLASF 387

BLAST of Cp4.1LG05g04270 vs. TrEMBL
Match: A5BM21_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041831 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 7.3e-98
Identity = 201/442 (45.48%), Postives = 270/442 (61.09%), Query Frame = 1

Query: 2   IEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKIC 61
           +EKILRIQPNVKKL+LLLRAAD   A +R +NEV+ K+LF+VLR KWG+NLN+ ISEK+ 
Sbjct: 29  VEKILRIQPNVKKLFLLLRAADTKSATQRLHNEVLGKELFRVLRDKWGSNLNSFISEKVT 88

Query: 62  LVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRFA 121
            + G+IS   +G+ +    +E+  +V++++NLAATT FDERYDVAL  NT+G  HV+ F+
Sbjct: 89  PISGDISCENLGVTNLNLREEIWREVDVILNLAATTKFDERYDVALGINTLGASHVLNFS 148

Query: 122 KECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKG--------------LDIEE 181
           K+C  LK+L+HVSTAYVSGER+G+ILES  KMG++LNG  G               ++ E
Sbjct: 149 KKCVKLKMLLHVSTAYVSGEREGLILESPLKMGKALNGASGLDVDKEKKLVEEGLNELNE 208

Query: 182 EQAI-----------------------------AMGEMTIGDLKNDLPLIIIRPTIVTST 241
            QA                              AMGEM +G  K +LPL+I+RPTI+TST
Sbjct: 209 LQATEETISLTMKELGMKRALMYGWPNTYVFTKAMGEMLLGQFKENLPLVILRPTIITST 268

Query: 242 YKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHEDQ 301
           Y EPF GW+EG+RTIDS++  Y KGKLTCF A     LD IP D+VVN MI+AM AH +Q
Sbjct: 269 YMEPFSGWIEGIRTIDSVLATYCKGKLTCFLADPECILDAIPGDMVVNCMIVAMVAHANQ 328

Query: 302 PSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLF--------- 361
           P +  IY VGSS +N +  +DL  F ++YF + P I+K+GKA+K+ +   F         
Sbjct: 329 PCEM-IYQVGSSLKNPLKLLDLHDFFFKYFHENPWINKDGKAVKVLQFLNFLLCQILXGM 388

Query: 362 ----NSMAK-----------------------FDDTNTERLRNDIRNKDTN-EAFVMDPK 364
               N   K                       FDD NT++LR       +  + F  DPK
Sbjct: 389 CXDHNRKIKMLMYLVELYKPYLFFKGIYAPYSFDDLNTDKLRLAATESSSKADLFYFDPK 448

BLAST of Cp4.1LG05g04270 vs. TrEMBL
Match: A0A067F4A8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g037479mg PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 8.3e-94
Identity = 185/358 (51.68%), Postives = 246/358 (68.72%), Query Frame = 1

Query: 2   IEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKIC 61
           +EKILR+QPNVKKLYLLLRAAD   A  RF+NE+I KDLF+VL++K G +LN LISEKI 
Sbjct: 29  VEKILRVQPNVKKLYLLLRAADAKSATVRFHNEIIGKDLFRVLKEKNGTHLNALISEKIT 88

Query: 62  LVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRFA 121
           LVPG+I+   +G+KDS   +EM N++++VINLAATT+FDERYDVALD NT G +HV+ F+
Sbjct: 89  LVPGDITREDLGVKDSNLREEMWNKLDVVINLAATTNFDERYDVALDLNTFGAKHVLNFS 148

Query: 122 KECRNLKLLVHVSTAYVSGERDGVILESSYKMGESL-----------NGVKGLDIEE--- 181
           K+C  L +LVHVSTA+V GE  G++ ESS+KMG++L             V   ++ E   
Sbjct: 149 KKCAKLIVLVHVSTAFVWGEESGMLYESSFKMGDTLNGASGLDIDVERQVVEQELNELQA 208

Query: 182 ----EQAIA-------------------------MGEMTIGDLKNDLPLIIIRPTIVTST 241
               E+AI                          MGEM I  LK ++ + I+RPTI+T T
Sbjct: 209 EGASEEAITLAMKDLGIKRAKIYGWPNTYVFTKVMGEMFIEHLKENMSVAIVRPTIITGT 268

Query: 242 YKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHEDQ 301
           YKEPFPGW EG+RTIDS+ VGY KG+LTCF       +D+IPAD+VVN +++A+ AH  Q
Sbjct: 269 YKEPFPGWAEGIRTIDSLAVGYGKGRLTCFLGDVKGIVDVIPADMVVNAIVVAVVAHAKQ 328

Query: 302 PSD-QTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLFNSMAKF 316
           PSD   IY VGSS RN + + +LQ ++Y+YFT+KP I+K+GK +K+GK+T+F+ MA F
Sbjct: 329 PSDVNNIYQVGSSLRNPLIYTNLQDYAYRYFTKKPWINKDGKPVKVGKITVFHDMASF 386

BLAST of Cp4.1LG05g04270 vs. TrEMBL
Match: M1ABM6_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400007405 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 7.8e-92
Identity = 179/358 (50.00%), Postives = 242/358 (67.60%), Query Frame = 1

Query: 2   IEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKIC 61
           +EKILR+QPNVKKLYLLLRAAD+  A +RF NEV+ KDLF+VLR+K G N  T +S++  
Sbjct: 29  VEKILRVQPNVKKLYLLLRAADDKSAMQRFNNEVVGKDLFKVLREKCGPNFTTFVSQRTT 88

Query: 62  LVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRFA 121
           +VPG+I+   +G+ D+  +++M  +V+IV+NLAATT+FDERYDVAL  NT G  HV+ FA
Sbjct: 89  IVPGDITCENLGVNDTNLLEQMWKEVDIVVNLAATTNFDERYDVALGLNTFGARHVLNFA 148

Query: 122 KECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKG----------------LDI 181
           K+C  LK+L+HVSTAYV GE++G++LE  Y MGE+LNG  G                L+ 
Sbjct: 149 KKCNKLKVLLHVSTAYVCGEKEGLMLEKPYYMGETLNGTLGLDIEAEKKVMDEKLKQLNA 208

Query: 182 EE--EQAI-------------------------AMGEMTIGDLKNDLPLIIIRPTIVTST 241
           E   E++I                         AMGEM +G LK ++PL+I RPTI+TST
Sbjct: 209 ENASEKSITTTMKELGLERARKYGWPNTYVFTKAMGEMLLGKLKEEVPLVINRPTIITST 268

Query: 242 YKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHEDQ 301
           +KEPFPGWVEG+RTIDS+ VGY KG++TCF     + LD+IPAD+VVN+MI+AM AH DQ
Sbjct: 269 FKEPFPGWVEGIRTIDSLAVGYGKGRITCFLGNPKTILDVIPADMVVNSMIVAMMAHADQ 328

Query: 302 PSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDK-NGKAIKLGKVTLFNSMAKF 316
              +TIY +GSS  N +   +L+ + + YF + P I+K +GK I +GKV + +SM  F
Sbjct: 329 KGSETIYQIGSSVSNPLNITNLRDYGFNYFRKNPWINKVDGKPIIVGKVNVLSSMDSF 386

BLAST of Cp4.1LG05g04270 vs. TAIR10
Match: AT3G44540.1 (AT3G44540.1 fatty acid reductase 4)

HSP 1 Score: 312.0 bits (798), Expect = 4.8e-85
Identity = 178/465 (38.28%), Postives = 257/465 (55.27%), Query Frame = 1

Query: 2   IEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWG-ANLNTLISEKI 61
           +EKILRIQP VKKL+LLLRAAD   A +RF++EV+EKDLF+VL+   G  NL   I+EK+
Sbjct: 28  VEKILRIQPKVKKLFLLLRAADNESAMQRFHSEVLEKDLFRVLKNALGDENLKAFITEKV 87

Query: 62  CLVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRF 121
             +PG+IS+  +G+K S  +  M N+++I++N+AATT+FDERYDV L  NT G  +V+ F
Sbjct: 88  VPIPGDISVDNLGVKGSDLLQHMWNEIDIIVNVAATTNFDERYDVGLSVNTFGPLNVLNF 147

Query: 122 AKECRNLKLLVHVSTAYVSGERDGV------------------ILESSYK-MGESLNGVK 181
           AK+C   +LL+HVSTAYV GE+ G+                  ++E+  + M + L  ++
Sbjct: 148 AKKCVKGQLLLHVSTAYVRGEKSGLLHEKTFHMGETLNGHRKLVIETEMELMKQKLKELQ 207

Query: 182 GLDIEEEQAI------------------------AMGEMTIGDLKNDLPLIIIRPTIVTS 241
             +  EE+                          +MGEM +G+ + +LP++IIRPT++TS
Sbjct: 208 KQNCSEEEISQSMKDLGMSRAKLHGWPNTYVFTKSMGEMLLGNYRENLPIVIIRPTMITS 267

Query: 242 TYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHED 301
           T+ EPFPGW+EG+RTIDS+IV Y KG+L CF A  NS LDLIP D+V N M+ A A H  
Sbjct: 268 TFSEPFPGWIEGLRTIDSVIVAYGKGRLKCFLADPNSVLDLIPVDMVANAMVTAAAIHAG 327

Query: 302 QPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLFNSMAKFDD 361
           +   QT+YHVGSS +N +T   +   +  YFT+ PL+ ++G +I + K T+ ++MA+F  
Sbjct: 328 KLGSQTVYHVGSSCKNPITFEQIHDLAASYFTKNPLVRRDGSSILVSKGTILSTMAQFSF 387

Query: 362 TNTERLR----------------NDIRNKDTNE--------------------------- 364
             T R +                N  + KD +                            
Sbjct: 388 YMTLRYKLPLQMLRLIYVIYPWWNGNKYKDIDRKIKLAMRLVDLYRPYVLFKGIFDDTNT 447

BLAST of Cp4.1LG05g04270 vs. TAIR10
Match: AT3G11980.1 (AT3G11980.1 Jojoba acyl CoA reductase-related male sterility protein)

HSP 1 Score: 235.7 bits (600), Expect = 4.4e-62
Identity = 136/368 (36.96%), Postives = 205/368 (55.71%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKI 60
           LIEK+LR+ P+V K+YLL++A  +  A +R  NEV++ +LF  L++  GA+  + +  K+
Sbjct: 146 LIEKVLRMAPDVSKIYLLIKAKSKEAAIERLKNEVLDAELFNTLKETHGASYMSFMLTKL 205

Query: 61  CLVPGEISLSQMGLK-DSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVR 120
             V G I  S +GL+ DS   +E+  +V+++IN AA T F+ERYDVALD NT G  +++ 
Sbjct: 206 IPVTGNICDSNIGLQADS--AEEIAKEVDVIINSAANTTFNERYDVALDINTRGPGNLMG 265

Query: 121 FAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGE------------------------ 180
           FAK+C+ LKL + VSTAYV+G+R G I+E  + MG+                        
Sbjct: 266 FAKKCKKLKLFLQVSTAYVNGQRQGRIMEKPFSMGDCIATENFLEGNRKALDVDREMKLA 325

Query: 181 ------------SLNGVKGLDIEEEQAI----------AMGEMTIGDLKNDLPLIIIRPT 240
                           +K L +E  ++           AMGEM I   + D+P++IIRP+
Sbjct: 326 LEAARKGTQNQDEAQKMKDLGLERARSYGWQDTYVFTKAMGEMMINSTRGDVPVVIIRPS 385

Query: 241 IVTSTYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMA 300
           ++ STYK+PFPGW+EG R +D I++ Y KG+LT F       LD++PAD+VVN  + A+A
Sbjct: 386 VIESTYKDPFPGWMEGNRMMDPIVLCYGKGQLTGFLVDPKGVLDVVPADMVVNATLAAIA 445

Query: 301 AH----EDQPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLF 318
            H     D   +  +Y + SSA N +   DL    Y ++   P +D  G  I +  + LF
Sbjct: 446 KHGMAMSDPEPEINVYQIASSAINPLVFEDLAELLYNHYKTSPCMDSKGDPIMVRLMKLF 505

BLAST of Cp4.1LG05g04270 vs. TAIR10
Match: AT4G33790.1 (AT4G33790.1 Jojoba acyl CoA reductase-related male sterility protein)

HSP 1 Score: 204.9 bits (520), Expect = 8.3e-53
Identity = 114/252 (45.24%), Postives = 161/252 (63.89%), Query Frame = 1

Query: 2   IEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKIC 61
           +EKILR+ PNVKKLYLLLRA+    A +RF +E+++KDLF+VL++K+G NLN L SEKI 
Sbjct: 33  VEKILRVAPNVKKLYLLLRASKGKSATQRFNDEILKKDLFKVLKEKYGPNLNQLTSEKIT 92

Query: 62  LVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRFA 121
           +V G+I L  +GL+D     EM +QV+ ++NLAATT FDERYDVAL  NT+G  +V+ FA
Sbjct: 93  IVDGDICLEDLGLQDFDLAHEMIHQVDAIVNLAATTKFDERYDVALGINTLGALNVLNFA 152

Query: 122 KECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKGLDIEEEQAIAMGEMTIGDL 181
           K C  +K+LVHVSTAYV GE+ G+I+E+ Y+MGE+LNG  GLDI  E+ +   ++     
Sbjct: 153 KRCAKVKILVHVSTAYVCGEKSGLIMETPYRMGETLNGTTGLDINYEKKLVQEKL----- 212

Query: 182 KNDLPLIIIRPTIVTSTYKE------PFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSS 241
            + L +I   P  +T T K+         GW       ++ +   A G++       N S
Sbjct: 213 -DQLRVIGAAPETITETMKDLGLRRAKMYGWP------NTYVFTKAMGEMMVGTKRENLS 272

Query: 242 LDLIPADIVVNT 248
           L L+   I+ +T
Sbjct: 273 LVLLRPSIITST 272

BLAST of Cp4.1LG05g04270 vs. TAIR10
Match: AT5G22420.1 (AT5G22420.1 fatty acid reductase 7)

HSP 1 Score: 191.8 bits (486), Expect = 7.3e-49
Identity = 104/259 (40.15%), Postives = 146/259 (56.37%), Query Frame = 1

Query: 100 DERYDVALDTNTIGVEHVVRFAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNG 159
           ++RYDVAL  NT G  +V+ FAK+C   KLL+HVST YV GER G I+E  + MGESLNG
Sbjct: 54  EQRYDVALGINTFGAINVLNFAKKCVKPKLLLHVSTVYVCGERPGHIVEKHFAMGESLNG 113

Query: 160 VKGL----------------------DIEEEQAI---------------------AMGEM 219
              +                      + E EQA+                     AMGEM
Sbjct: 114 KNKVDINTERRLADQKSKQFKEQGCSEEETEQAMKDFGLKRARLYGWPNTYVFTKAMGEM 173

Query: 220 TIGDLKNDLPLIIIRPTIVTSTYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSL 279
            +G  +  +P++IIRPTI+TST+ +PFPGW+EG++T+DS+I+ Y KG L CF     +  
Sbjct: 174 LLGHYRETMPIVIIRPTIITSTFSDPFPGWIEGLKTVDSVIIFYGKGILKCFLVDQKTVC 233

Query: 280 DLIPADIVVNTMIMAMAAHEDQPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDK 316
           D+IP D+VVN MI   A H       T+YHVGSS +N + +  +     +YF + PL+ +
Sbjct: 234 DIIPVDMVVNAMIAIAADHCHDSGSHTVYHVGSSNQNPVIYKQIYEMMSRYFMKSPLVGR 293

BLAST of Cp4.1LG05g04270 vs. TAIR10
Match: AT5G22500.1 (AT5G22500.1 fatty acid reductase 1)

HSP 1 Score: 186.0 bits (471), Expect = 4.0e-47
Identity = 95/169 (56.21%), Postives = 129/169 (76.33%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGA-NLNTLISEK 60
           L+EKILR+QPNVKK+YLLLRA DE  A +R  +EV+E DLF+VLR   G  NLN L+ EK
Sbjct: 27  LVEKILRLQPNVKKIYLLLRAPDEKSAMQRLRSEVMEIDLFKVLRNNLGEDNLNALMREK 86

Query: 61  ICLVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVR 120
           I  VPG+IS+  +GLKD+  +  M ++++I+IN+AATT+FDERYD+ L  NT G  +V+ 
Sbjct: 87  IVPVPGDISIDNLGLKDTDLIQRMWSEIDIIINIAATTNFDERYDIGLGINTFGALNVLN 146

Query: 121 FAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKGLDIEEE 169
           FAK+C   +LL+HVSTAY+SGE+ G++LE  +KMGE+L+G + LDI  E
Sbjct: 147 FAKKCVKGQLLLHVSTAYISGEQPGLLLEKPFKMGETLSGDRELDINIE 195

BLAST of Cp4.1LG05g04270 vs. NCBI nr
Match: gi|449456126|ref|XP_004145801.1| (PREDICTED: fatty acyl-CoA reductase 3 [Cucumis sativus])

HSP 1 Score: 459.5 bits (1181), Expect = 5.3e-126
Identity = 237/359 (66.02%), Postives = 278/359 (77.44%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKI 60
           L+EKILR+QPNVKKLYLLLRA DE  AKKRF+NEV+EK LFQVL+KK GANLNTLISEKI
Sbjct: 22  LVEKILRVQPNVKKLYLLLRADDEITAKKRFHNEVVEKALFQVLKKKHGANLNTLISEKI 81

Query: 61  CLVPGEISLSQMGLK-DSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVR 120
           CLVPGEISL QMGLK DSIW+D++K+QVEI+INLAATT+FDERYDVAL TN +G +HV+ 
Sbjct: 82  CLVPGEISLPQMGLKHDSIWIDKLKSQVEIIINLAATTNFDERYDVALGTNALGAKHVIN 141

Query: 121 FAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLN--------------------- 180
           FAK+C NLKL+VHVSTAYVSGER+G+I+E+ YKMGESLN                     
Sbjct: 142 FAKQCSNLKLVVHVSTAYVSGEREGLIMETPYKMGESLNWFKGLDIEVEQMVIEDKLKQL 201

Query: 181 ------------GVKGLDIEEEQAI----------AMGEMTIGDLKNDLPLIIIRPTIVT 240
                        +K L +E  +            AMGEM IGDLK+++PLI+IRPTIVT
Sbjct: 202 KEDGATNETITLAMKDLGLERSKLYGWPNTYVFTKAMGEMIIGDLKDEVPLIVIRPTIVT 261

Query: 241 STYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHE 300
           STYKEPFPGW+EGVRTIDS+IVGYAKGKLTCF AG NS +DLIPAD+VVNT+IMAM  H+
Sbjct: 262 STYKEPFPGWIEGVRTIDSLIVGYAKGKLTCFVAGINSIIDLIPADMVVNTIIMAMMVHK 321

Query: 301 DQPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLFNSMAKF 316
            QPS+  IYHVGSS RN + H D QRF+YQYFT+KPLID++G AIK+GKVTLF+ M  F
Sbjct: 322 LQPSNHIIYHVGSSKRNPIKHADFQRFNYQYFTKKPLIDRDGNAIKVGKVTLFDDMTNF 380

BLAST of Cp4.1LG05g04270 vs. NCBI nr
Match: gi|659117418|ref|XP_008458592.1| (PREDICTED: fatty acyl-CoA reductase 3-like [Cucumis melo])

HSP 1 Score: 458.0 bits (1177), Expect = 1.5e-125
Identity = 235/359 (65.46%), Postives = 277/359 (77.16%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKI 60
           L+EKILR+QPNVKKLYLLLRA DE  AKKRF+NEV+EK LFQVL+KK+GANLNTLISEKI
Sbjct: 22  LVEKILRVQPNVKKLYLLLRADDEITAKKRFHNEVVEKALFQVLKKKYGANLNTLISEKI 81

Query: 61  CLVPGEISLSQMGLK-DSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVR 120
           CLVPGEISL Q+GLK DSIW+D++K+QVEI+INLAATT+FDERYDVAL TN +G +HV+ 
Sbjct: 82  CLVPGEISLPQLGLKHDSIWIDKIKSQVEIIINLAATTNFDERYDVALGTNALGAKHVIN 141

Query: 121 FAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLN--------------------- 180
           FAK+C NLKL+VHVSTAYVSGER+G+I+E+ YKMGESLN                     
Sbjct: 142 FAKQCSNLKLVVHVSTAYVSGEREGLIMETPYKMGESLNWMKGLDIEAEQMVIEDKLKQL 201

Query: 181 ------------GVKGLDIEEEQAI----------AMGEMTIGDLKNDLPLIIIRPTIVT 240
                        +K L +E  +            AMGEM IGDLK+D+PLI+IRPTIVT
Sbjct: 202 KENGATNETITLAMKDLGLERSKLYGWPNTYVFTKAMGEMIIGDLKDDVPLIVIRPTIVT 261

Query: 241 STYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHE 300
           STYKEPFPGW+EGVRTIDS+IVGYAKGKLTCF AG NS +DLIPAD+VVNT+I AM  H+
Sbjct: 262 STYKEPFPGWIEGVRTIDSLIVGYAKGKLTCFVAGINSVIDLIPADMVVNTIITAMMVHK 321

Query: 301 DQPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLFNSMAKF 316
            QPS+  IYHVGSS RN + H D QRF+YQYFT+KPLID++G AIK+GKVT+F  M  F
Sbjct: 322 LQPSNHIIYHVGSSTRNPIRHADFQRFNYQYFTKKPLIDRDGNAIKVGKVTIFGDMTNF 380

BLAST of Cp4.1LG05g04270 vs. NCBI nr
Match: gi|700191740|gb|KGN46944.1| (hypothetical protein Csa_6G151810 [Cucumis sativus])

HSP 1 Score: 450.7 bits (1158), Expect = 2.5e-123
Identity = 237/371 (63.88%), Postives = 278/371 (74.93%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKI 60
           L+EKILR+QPNVKKLYLLLRA DE  AKKRF+NEV+EK LFQVL+KK GANLNTLISEKI
Sbjct: 22  LVEKILRVQPNVKKLYLLLRADDEITAKKRFHNEVVEKALFQVLKKKHGANLNTLISEKI 81

Query: 61  CLVPGEISLSQMGLK-DSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVR 120
           CLVPGEISL QMGLK DSIW+D++K+QVEI+INLAATT+FDERYDVAL TN +G +HV+ 
Sbjct: 82  CLVPGEISLPQMGLKHDSIWIDKLKSQVEIIINLAATTNFDERYDVALGTNALGAKHVIN 141

Query: 121 FAKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLN--------------------- 180
           FAK+C NLKL+VHVSTAYVSGER+G+I+E+ YKMGESLN                     
Sbjct: 142 FAKQCSNLKLVVHVSTAYVSGEREGLIMETPYKMGESLNWFKGLDIEVEQMVIEDKLKQL 201

Query: 181 ------------GVKGLDIEEEQAI----------AMGEMTIGDLKNDLPLIIIRPTIVT 240
                        +K L +E  +            AMGEM IGDLK+++PLI+IRPTIVT
Sbjct: 202 KEDGATNETITLAMKDLGLERSKLYGWPNTYVFTKAMGEMIIGDLKDEVPLIVIRPTIVT 261

Query: 241 STYKEPFPGWVEGVR------------TIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIV 300
           STYKEPFPGW+EGVR            TIDS+IVGYAKGKLTCF AG NS +DLIPAD+V
Sbjct: 262 STYKEPFPGWIEGVRIWGQTLCINLCRTIDSLIVGYAKGKLTCFVAGINSIIDLIPADMV 321

Query: 301 VNTMIMAMAAHEDQPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLG 316
           VNT+IMAM  H+ QPS+  IYHVGSS RN + H D QRF+YQYFT+KPLID++G AIK+G
Sbjct: 322 VNTIIMAMMVHKLQPSNHIIYHVGSSKRNPIKHADFQRFNYQYFTKKPLIDRDGNAIKVG 381

BLAST of Cp4.1LG05g04270 vs. NCBI nr
Match: gi|449456128|ref|XP_004145802.1| (PREDICTED: alcohol-forming fatty acyl-CoA reductase-like [Cucumis sativus])

HSP 1 Score: 400.6 bits (1028), Expect = 2.9e-108
Identity = 211/358 (58.94%), Postives = 260/358 (72.63%), Query Frame = 1

Query: 1   LIEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKI 60
           L+EKILRIQPNVKKLYLLLRA DET A +RF NEV+ KDLF+VL++KWGANL++ ISEK+
Sbjct: 30  LLEKILRIQPNVKKLYLLLRATDETTAMQRFCNEVMGKDLFKVLKEKWGANLDSFISEKV 89

Query: 61  CLVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRF 120
            +V  +IS+S MGLKDSI V+E+K+ V+I+INLAATT+F+ERYDVAL TNT+G +HVV F
Sbjct: 90  SVVSSDISVSDMGLKDSILVEEIKHNVQIIINLAATTNFNERYDVALGTNTLGAKHVVDF 149

Query: 121 AKECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKGLDIEEEQ----------- 180
           AK+C N KLLVHVSTAY+SGE++G ILE+ +K+GESLNG +GL+IE EQ           
Sbjct: 150 AKQCPNFKLLVHVSTAYISGEKEGFILETPHKLGESLNGTEGLNIEIEQKIIEQRLKQLK 209

Query: 181 --------------------------------AIAMGEMTIGDLKNDLPLIIIRPTIVTS 240
                                             AMGEM I DLK++LPLIIIRPTI+TS
Sbjct: 210 DNGASEKDVTIAMKDLGIQRANQYGWPNTYVFTKAMGEMLINDLKHNLPLIIIRPTIITS 269

Query: 241 TYKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHED 300
           TYKEPF GW+EG RTIDS+IVGYAKGKLT     TNS +D+IPAD+VVNT+I  M  H+ 
Sbjct: 270 TYKEPFSGWIEGTRTIDSLIVGYAKGKLTFIPCDTNSIIDVIPADMVVNTIIATMVMHKL 329

Query: 301 QPSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLFNSMAKF 316
           Q     IYHVGSS RN M   DLQRF  +YFT+KP I+ +G AIK+ K+T+FN++A F
Sbjct: 330 QYRQTIIYHVGSSTRNPMKIDDLQRFICKYFTEKPWINGDGNAIKVKKITVFNNLASF 387

BLAST of Cp4.1LG05g04270 vs. NCBI nr
Match: gi|147852421|emb|CAN81280.1| (hypothetical protein VITISV_041831 [Vitis vinifera])

HSP 1 Score: 365.5 bits (937), Expect = 1.0e-97
Identity = 201/442 (45.48%), Postives = 270/442 (61.09%), Query Frame = 1

Query: 2   IEKILRIQPNVKKLYLLLRAADETEAKKRFYNEVIEKDLFQVLRKKWGANLNTLISEKIC 61
           +EKILRIQPNVKKL+LLLRAAD   A +R +NEV+ K+LF+VLR KWG+NLN+ ISEK+ 
Sbjct: 29  VEKILRIQPNVKKLFLLLRAADTKSATQRLHNEVLGKELFRVLRDKWGSNLNSFISEKVT 88

Query: 62  LVPGEISLSQMGLKDSIWVDEMKNQVEIVINLAATTHFDERYDVALDTNTIGVEHVVRFA 121
            + G+IS   +G+ +    +E+  +V++++NLAATT FDERYDVAL  NT+G  HV+ F+
Sbjct: 89  PISGDISCENLGVTNLNLREEIWREVDVILNLAATTKFDERYDVALGINTLGASHVLNFS 148

Query: 122 KECRNLKLLVHVSTAYVSGERDGVILESSYKMGESLNGVKG--------------LDIEE 181
           K+C  LK+L+HVSTAYVSGER+G+ILES  KMG++LNG  G               ++ E
Sbjct: 149 KKCVKLKMLLHVSTAYVSGEREGLILESPLKMGKALNGASGLDVDKEKKLVEEGLNELNE 208

Query: 182 EQAI-----------------------------AMGEMTIGDLKNDLPLIIIRPTIVTST 241
            QA                              AMGEM +G  K +LPL+I+RPTI+TST
Sbjct: 209 LQATEETISLTMKELGMKRALMYGWPNTYVFTKAMGEMLLGQFKENLPLVILRPTIITST 268

Query: 242 YKEPFPGWVEGVRTIDSIIVGYAKGKLTCFAAGTNSSLDLIPADIVVNTMIMAMAAHEDQ 301
           Y EPF GW+EG+RTIDS++  Y KGKLTCF A     LD IP D+VVN MI+AM AH +Q
Sbjct: 269 YMEPFSGWIEGIRTIDSVLATYCKGKLTCFLADPECILDAIPGDMVVNCMIVAMVAHANQ 328

Query: 302 PSDQTIYHVGSSARNSMTHIDLQRFSYQYFTQKPLIDKNGKAIKLGKVTLF--------- 361
           P +  IY VGSS +N +  +DL  F ++YF + P I+K+GKA+K+ +   F         
Sbjct: 329 PCEM-IYQVGSSLKNPLKLLDLHDFFFKYFHENPWINKDGKAVKVLQFLNFLLCQILXGM 388

Query: 362 ----NSMAK-----------------------FDDTNTERLRNDIRNKDTN-EAFVMDPK 364
               N   K                       FDD NT++LR       +  + F  DPK
Sbjct: 389 CXDHNRKIKMLMYLVELYKPYLFFKGIYAPYSFDDLNTDKLRLAATESSSKADLFYFDPK 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
FACR4_ARATH8.6e-8438.28Probable fatty acyl-CoA reductase 4 OS=Arabidopsis thaliana GN=FAR4 PE=2 SV=1[more]
FACR2_ARATH7.8e-6136.96Fatty acyl-CoA reductase 2 OS=Arabidopsis thaliana GN=FAR2 PE=2 SV=2[more]
FACR3_ARATH1.5e-5145.24Fatty acyl-CoA reductase 3 OS=Arabidopsis thaliana GN=FAR3 PE=2 SV=1[more]
FACR7_ARATH1.3e-4740.15Putative fatty acyl-CoA reductase 7 OS=Arabidopsis thaliana GN=FAR7 PE=3 SV=1[more]
FACR1_ARATH7.1e-4656.21Fatty acyl-CoA reductase 1 OS=Arabidopsis thaliana GN=FAR1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KDN1_CUCSA1.7e-12363.88Uncharacterized protein OS=Cucumis sativus GN=Csa_6G151810 PE=4 SV=1[more]
A0A0A0KB44_CUCSA2.0e-10858.94Uncharacterized protein OS=Cucumis sativus GN=Csa_6G152310 PE=4 SV=1[more]
A5BM21_VITVI7.3e-9845.48Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041831 PE=4 SV=1[more]
A0A067F4A8_CITSI8.3e-9451.68Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g037479mg PE=4 SV=1[more]
M1ABM6_SOLTU7.8e-9250.00Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400007405 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G44540.14.8e-8538.28 fatty acid reductase 4[more]
AT3G11980.14.4e-6236.96 Jojoba acyl CoA reductase-related male sterility protein[more]
AT4G33790.18.3e-5345.24 Jojoba acyl CoA reductase-related male sterility protein[more]
AT5G22420.17.3e-4940.15 fatty acid reductase 7[more]
AT5G22500.14.0e-4756.21 fatty acid reductase 1[more]
Match NameE-valueIdentityDescription
gi|449456126|ref|XP_004145801.1|5.3e-12666.02PREDICTED: fatty acyl-CoA reductase 3 [Cucumis sativus][more]
gi|659117418|ref|XP_008458592.1|1.5e-12565.46PREDICTED: fatty acyl-CoA reductase 3-like [Cucumis melo][more]
gi|700191740|gb|KGN46944.1|2.5e-12363.88hypothetical protein Csa_6G151810 [Cucumis sativus][more]
gi|449456128|ref|XP_004145802.1|2.9e-10858.94PREDICTED: alcohol-forming fatty acyl-CoA reductase-like [Cucumis sativus][more]
gi|147852421|emb|CAN81280.1|1.0e-9745.48hypothetical protein VITISV_041831 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0080019fatty-acyl-CoA reductase (alcohol-forming) activity
Vocabulary: INTERPRO
TermDefinition
IPR026055FAR
IPR016040NAD(P)-bd_dom
IPR013120Male_sterile_NAD-bd
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008152 metabolic process
biological_process GO:0010025 wax biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0005783 endoplasmic reticulum
molecular_function GO:0080019 fatty-acyl-CoA reductase (alcohol-forming) activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g04270.1Cp4.1LG05g04270.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013120Male sterility, NAD-bindingPFAMPF07993NAD_binding_4coord: 1..248
score: 2.4
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 66..276
score: 2.4
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 76..282
score: 1.05
IPR026055Fatty acyl-CoA reductasePANTHERPTHR11011MALE STERILITY PROTEIN 2-RELATEDcoord: 1..363
score: 1.0E
NoneNo IPR availablePANTHERPTHR11011:SF25FATTY ACYL-COA REDUCTASE 3-RELATEDcoord: 1..363
score: 1.0E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG05g04270Cp4.1LG16g08200Cucurbita pepo (Zucchini)cpecpeB319