Cp4.1LG05g03930 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG05g03930
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPhosphatidylinositol glycan anchor biosynthesis class U protein
LocationCp4.1LG05 : 1723518 .. 1728746 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCGAAGGAACAATTCGAAGAGGAGGAGAAGAAAAGGAAGGGAAACTTTTGGATATGGATGGTATTGTCAGTGATTTTTAGGATCGTTATGATATATTTTCCCAATCTGAATCTGTCCTCTCGCCCCGAAGTCTCCACACCTCTAACCAGCATTCATCGACGTATTTTACTCACTTTTCTTTTGAGATTCTAGCTATGATTGGTTTCTAGCTTAGTTTATGCTCATGATATCTTGTTGATTGTTAAATGTCTTGCAGTAGCTGAGGGCTATTGGTTAAAGCAGTCGTCAATGTCTCCCTATGCAGGTTTCCTTGTTTTCCTTTCGTTATCAATTCAAATGTTGCTTCTGAAGCTGGCTGATTGTAGATGGTTATCTTATAATTTTGAGAATTTGGTTATTTTTCTGAGCAGGATCTATGTACCATGGCTCTCCTCTGTTACTGTCTCTTCTAGGGCCACTGACTGTCAGAAGGTAGAAACCAACCTCCAAAAGAAATGTAACATTGTACTTCAATGGAGGCTTTTTTTTCTCCTTGTTGGTTTAACAGGTGTCTGTGTTTTGTATTTATAATGGCTAAGAACCTGAAGCTGATTAGTTTTTCGTTTCTTCTATCGTTTTTCTTATTGCATACTTAAATTGAAGAGTTTGAAGCCCGTGAAAATGAATTTGGTAACTTCAGAATTTCTGAATTACTAGGATCCTGAGCTTGCAAGTGCTCGGAGTATAATGCCTAAGTTTGGTGTGTTTTCTGACATTCAATAATAGTTGTATTTTTGTGATGCAGAATTGAAGGGCAACCAGACCATCTTTTATGCAGGTGAATTTTGAATTCTTTTTTTGTTCGCCCCTAAGATTGATTTATGCACTTTTAAGGTTGTTATCAATGGTTTGAAAGTGAATGGGTTTCAGTGAAGTCCAATTTCTTTTAGCATTTCTGCTGTGCACTTTTTGTACTTAAATTATTTGTGATTTCTTCAAGGCCTAAGATTTTCATTAATTAATGATAGTTCTGTTATTTATTCAGGCTGTTAATATAAATGTATTGTTGCATAGTTTTGCTTTTGTGGTTGCAGATGTTCTGAGTGCATTGCTTATTCGTGCAGTCGGTCAGAATCTTCAGAGGGCATATTATCGGAATTTGAGATTGCTGAAGGTTAACCTATCAAAAAACTCAGGTACTTGACATGCGATTGAAAATTATGCATATGATGCACTGTAAATGAATGCCGATCTTTATTCTAGTTAAGACTGAACTGCATACAATCATTAGTGACGGAAAGGAGAGATCAGATATGATCATTGATCCCACAAGTTAGGACATTTTAATTAAAAAATACAGGGTGAAGTTTCCTTGACATTTTCCTGTGTAACGTCAGGGATCTAAATGCTATGCCATAAATTTTAAAATTAACTTCTAGGAATTCTATTTGGAATATATTTTTACAAACTTTAGGATTCCATGAACATCTTAAAGATGATTATTGACTCTTTATCCATGAACAATATATCGCGATAACCATAGTATAACCTATATATAGCTTATAGTAATTAAAAAATTACTTCACGAAAATTATAACCACAATTAATTGTCATCTTGCATTTCTCTTATAAGGTTGGGTTCGTACGCTGAAGTAATTTGTATTGATTTTAACTCTGCTTTGTAAATTTCTGTTGCATTCTCTGTACTCCTTTCGTTTGAAACTTGTTTCTGATAATACAAATATCACAGAAAGCCATTATTTGCTTGAAATGAAAACTCCCCTGAAACTGAATAAGATGTTGGCCCTCAGACTTTCATCTATTCTCGTTCTATAATCTTCTTAAAGAACAGGTATTTTCTGAATAAATGAAGTAAGTTTAAGATGCAATCTAATGCCTTTGGATATAGGTTTCCTTTGAACTGTTGAAGCATGATACAGAAATCAGTTACTCAGCATGGTGCTGATCTGAGATGTATTAAACCTTGTATAGAAGATAATATAATGTTCAATGCCCAATTGAGTTCAAATATAACTCGTACGTTCAGACAACTATGTCAATTTGAATGGATGTGGTTAGTGGACTTTAGAATTGAAATCCTTTCTACATAAGGGAAGCGCACAATTGCAGTTTCACTATCTAGTTATATCTGTCTTGACCTATATGATTACTGTAGATTCTTTCTAAATGATGGAGCTGGGAAGTGAGGACTTGGAAATCACTTATTAGGTTGATATAGCAATACGGATATTCAATTCTTACTGTTGGTTTATGTTTGTGATGTTTTTCATAGTTAGATTTATTGGCTATAATTATATTTTAATTATTTGTATAAGTTTTTTCACTTTTACGTATTAGACCTTTTTTATTCTTAAAAAATGTCCCTTATAACTATTGTAAGTAAATGAGAATTGACAATTATATTATTCCATAGAAATTGATATTTCACACTTACAAAGGGAGAAATTGGGAGAAACACATGAAAACATGGTCTTTTTGGTTGCTGTAGTAATAATAATATTTTTTAAGGAAACAGAAATTTCATTGATGAATATAAAATTACGAAGGGGAAAGCTCTGTTTTCTGGATGACTTAATTATAGATCTGGATTGTCTTATAGTTGTATGTCATTGTCATTCAAATGAGAACTGCCTTTCGAGTTGGATTTCAACTTGTTGAATGCTACATTCCTTTCTTTTATTGGCTTATGAGTAATGTCATTATTTGTTTAATGTTAGCAGAGTTCTTCCCAGCTGGAGATATTGCTTCTCTTGTGTACCTGTGGAATCCTTTCACAATTGTTGCATGTGTGGGTTTGTCCACATCTCCAATCGAAAATCTAGCCATTGTGCTTACGCTCTATGGAGCATCTAAAGGCAAGAAATTAGCAATCTTTGCTTTGTAATGACAATCTTTTTTCTTCCGCCTTCTGAAAAAGCATAATTTATAGACAAGAGTCATACACGTGAATTTCACCAAAACTTCAAAGGTTTGCTGTTCATATTGAAGCACCAGCAAATTGACATTTTTCATACCACCATTACACTGATTGCATGTTTTTGTTACTGAAAATATACATTTATTTTTGTTATGATTTTCTTTTTGGTTATTTTGAACCATTCATTTGATAAGATGCATCTAATGTAGTACAGGACAAGTTCCATTGGCTGCTTTTGGATTTGTGGTGGCGACTCATCTATCTCTTTATCCTATAATTCTTATAATTCCGGTATGATTGTTCTGTCTGGGTGTTTGTAAGACAATATATAATTTGCTGGCTCATGGATTCTTCTAATATAGTTGCTATGTTCTCCTTCCCTCCAGGTAGTCCTTCTACTAGGTAATGGCTTAGATGCTCCTCCTAGGAAATTGTTCTTGCAAAGGAGTAGTAGTAGAGTTGTTGTGAGACCTTCACGTGATAGCTGTAGCCAGCCGGAGGAAGTGATCAATCAGTCTGAAGTACCAAATGGTTTCTCATTGAGACCAGTGATGTATTTCTTATTATGGGTTTCTTTATTTTCTGTGTATCTGCTGCTTCTCTGTGGCATATCTCTTAAACAATTTGGTGGACTCTGGGAGATGTTTAGAAGGTATGGATTTTTTATTTAGAACTTTCTGCCATATATTTATCAATGTACCCGTTGACACTTCGCATTTCTCATACCAAAATGAACATATGCCTACTTCACCCAGTATACAAGTGCGATTGTGATAGGTCAGTGATGAATATGCACATTTCAGGCCCTCCTTCACCTTCCTTAGTCTTTAAGGATGGGGAGGTGTCACTTGGCTGAATGCTAGTCAGTTTCCAAAATACTGTGTATATTCATTTCATTAATATGGAAAGAAAAGGTCAACAACCTGGAAGTTAAATCCACCAGTGAGAAAGTTGGTTCCTTTTCTTTTATTCTTTTGATTTATTTATTTATAATTTTTTTATATCAAGGTTTCACGTTTCTAAGTAACCACTCTTATTACCATGCAAGTAACATGAGTTTGAAGTGTTTATCTGAGTTGCAACCATATTTCTTACTCGGCTATAAATGTGTGGTTGGTTTCTGCAGTACATATGGCTTTCTTCTCACTGTGCAAGATTTGTCTCCAAATATTGGGGTTCTATGGTGAGTCCAACAAAATACTAGTTGGTTACAAATGAAACCTATACATAAAGTCTTACACGTGACTTTCCAACTGATTTTTTTTAATGCTGTTTTCAGGTACCTTTTTGCTGAAGTGTTTGAGTTCTTCAGGGATTTTTATTTAATAGTCTTCCATATCAATATTCTTTTTATGATAACACCATTGGCTGTTCGTCTCCACCATCGACCCTTGTTTCTGGCTTTTGTGCTTCTTTCCATTTCTGCAATGTTGAAGTCTTATCCTTCGGTATCCTCACGTCTTGTTGCATGACTTTCTTTTGATTTTTTAGTATCATGCACTGACTACAGGATGATGCGAGTTTAATAAGCAATCTTGTCTTGTCATTTTGACTCCAATAGGTAGGGGACTCAGCTCTGTACTTGAGTTTTATGGGATTGTTTGTATACGAATTAGTTGGTGAGTAATCATGCGGTTCTGGTCACTTGTATTTTCAACCTGATTTGAATCTTCACCTTTTAATTAATGTCCAATGCTATTCATACAGATTTGGAGTTCTCATTCTTTCTGTTCTGCGGGTATATTGGGATTTCACTGCTCAGCCCTGTAATGCACAATCTTTGGATATGGAGGGTATGCCTTCTTTGCATCCTGATTTGAACAGAAACATGGAAAGACTTGAATCTCTTTTTAACTAAATGACATCACGAAATTGCAGGGGACTGGCAATGCAAATTTCTATTTTGCAAACGCTATGGCTTATGCTTGCTTCCAGGTTGGTCTTTCTTTTTTAAAATATAATCTGCATTCATATCACTGCTAGGATTCTCCACGTTCCACTTGCTTAAAATATAATTTGCATTCATATCACTGCTTGGACCTAATACACGAAAAGCTGTGCCTATCTTGCTTTCTAGTCACTACTAATCGCTGTTGTTAGGACAGACATATTTCGGTCAAGAACATGGTTTTGTTGTCATTCGATTACTTGTATAGATGATCTTTAGACCAATTTGTTATATGTTCAGTAATTCTCTGGGCCCACTACCTGTTTCCTTACAGATCGTTCTAGTGGTTGAGAGCGTAAGTACAATGCTGAATCACGACCGGAAACTGAGGAAGCTGTCTGCAGTGAAGCTTTCATGA

mRNA sequence

ATGGCGGCGAAGGAACAATTCGAAGAGGAGGAGAAGAAAAGGAAGGGAAACTTTTGGATATGGATGGTATTGTCAGTGATTTTTAGGATCGTTATGATATATTTTCCCAATCTGAATCTGTCCTCTCGCCCCGAAGTCTCCACACCTCTAACCAGCATTCATCGACTAGCTGAGGGCTATTGGTTAAAGCAGTCGTCAATGTCTCCCTATGCAGGTTTCCTTGTTTTCCTTTCGTTATCAATTCAAATGTTGCTTCTGAAGCTGGCTGATTGTAGATGGTTATCTTATAATTTTGAGAATTTGGTTATTTTTCTGAGCAGGATCTATGTACCATGGCTCTCCTCTGTTACTGTCTCTTCTAGGGCCACTGACTGTCAGAAGGATCCTGAGCTTGCAAGTGCTCGGAGTATAATGCCTAAGTTTGATGTTCTGAGTGCATTGCTTATTCGTGCAGTCGGTCAGAATCTTCAGAGGGCATATTATCGGAATTTGAGATTGCTGAAGGTTAACCTATCAAAAAACTCAGAGTTCTTCCCAGCTGGAGATATTGCTTCTCTTGTGTACCTGTGGAATCCTTTCACAATTGTTGCATGTGTGGGTTTGTCCACATCTCCAATCGAAAATCTAGCCATTGTGCTTACGCTCTATGGAGCATCTAAAGGCAAGAAATTAGCAATCTTTGCTTTTACAGGACAAGTTCCATTGGCTGCTTTTGGATTTGTGGTGGCGACTCATCTATCTCTTTATCCTATAATTCTTATAATTCCGGTAGTCCTTCTACTAGGTAATGGCTTAGATGCTCCTCCTAGGAAATTGTTCTTGCAAAGGAGTAGTAGTAGAGTTGTTGTGAGACCTTCACGTGATAGCTGTAGCCAGCCGGAGGAAGTGATCAATCAGTCTGAAGTACCAAATGGTTTCTCATTGAGACCAGTGATGTATTTCTTATTATGGGTTTCTTTATTTTCTGTGTATCTGCTGCTTCTCTGTGGCATATCTCTTAAACAATTTGGTGGACTCTGGGAGATGTTTAGAAGTACATATGGCTTTCTTCTCACTGTGCAAGATTTGTCTCCAAATATTGGGGTTCTATGGTACCTTTTTGCTGAAGTGTTTGAGTTCTTCAGGGATTTTTATTTAATAGTCTTCCATATCAATATTCTTTTTATGATAACACCATTGGCTGTTCGTCTCCACCATCGACCCTTGTTTCTGGCTTTTGTGCTTCTTTCCATTTCTGCAATGTTGAAGTCTTATCCTTCGGTAGGGGACTCAGCTCTGTACTTGAGTTTTATGGGATTGTTTGTATACGAATTAGTTGATTTGGAGTTCTCATTCTTTCTGTTCTGCGGGTATATTGGGATTTCACTGCTCAGCCCTGTAATGCACAATCTTTGGATATGGAGGGGGACTGGCAATGCAAATTTCTATTTTGCAAACGCTATGGCTTATGCTTGCTTCCAGATGATCTTTAGACCAATTTGTTATATGTTCAGTAATTCTCTGGGCCCACTACCTGTTTCCTTACAGATCGTTCTAGTGGTTGAGAGCGTAAGTACAATGCTGAATCACGACCGGAAACTGAGGAAGCTGTCTGCAGTGAAGCTTTCATGA

Coding sequence (CDS)

ATGGCGGCGAAGGAACAATTCGAAGAGGAGGAGAAGAAAAGGAAGGGAAACTTTTGGATATGGATGGTATTGTCAGTGATTTTTAGGATCGTTATGATATATTTTCCCAATCTGAATCTGTCCTCTCGCCCCGAAGTCTCCACACCTCTAACCAGCATTCATCGACTAGCTGAGGGCTATTGGTTAAAGCAGTCGTCAATGTCTCCCTATGCAGGTTTCCTTGTTTTCCTTTCGTTATCAATTCAAATGTTGCTTCTGAAGCTGGCTGATTGTAGATGGTTATCTTATAATTTTGAGAATTTGGTTATTTTTCTGAGCAGGATCTATGTACCATGGCTCTCCTCTGTTACTGTCTCTTCTAGGGCCACTGACTGTCAGAAGGATCCTGAGCTTGCAAGTGCTCGGAGTATAATGCCTAAGTTTGATGTTCTGAGTGCATTGCTTATTCGTGCAGTCGGTCAGAATCTTCAGAGGGCATATTATCGGAATTTGAGATTGCTGAAGGTTAACCTATCAAAAAACTCAGAGTTCTTCCCAGCTGGAGATATTGCTTCTCTTGTGTACCTGTGGAATCCTTTCACAATTGTTGCATGTGTGGGTTTGTCCACATCTCCAATCGAAAATCTAGCCATTGTGCTTACGCTCTATGGAGCATCTAAAGGCAAGAAATTAGCAATCTTTGCTTTTACAGGACAAGTTCCATTGGCTGCTTTTGGATTTGTGGTGGCGACTCATCTATCTCTTTATCCTATAATTCTTATAATTCCGGTAGTCCTTCTACTAGGTAATGGCTTAGATGCTCCTCCTAGGAAATTGTTCTTGCAAAGGAGTAGTAGTAGAGTTGTTGTGAGACCTTCACGTGATAGCTGTAGCCAGCCGGAGGAAGTGATCAATCAGTCTGAAGTACCAAATGGTTTCTCATTGAGACCAGTGATGTATTTCTTATTATGGGTTTCTTTATTTTCTGTGTATCTGCTGCTTCTCTGTGGCATATCTCTTAAACAATTTGGTGGACTCTGGGAGATGTTTAGAAGTACATATGGCTTTCTTCTCACTGTGCAAGATTTGTCTCCAAATATTGGGGTTCTATGGTACCTTTTTGCTGAAGTGTTTGAGTTCTTCAGGGATTTTTATTTAATAGTCTTCCATATCAATATTCTTTTTATGATAACACCATTGGCTGTTCGTCTCCACCATCGACCCTTGTTTCTGGCTTTTGTGCTTCTTTCCATTTCTGCAATGTTGAAGTCTTATCCTTCGGTAGGGGACTCAGCTCTGTACTTGAGTTTTATGGGATTGTTTGTATACGAATTAGTTGATTTGGAGTTCTCATTCTTTCTGTTCTGCGGGTATATTGGGATTTCACTGCTCAGCCCTGTAATGCACAATCTTTGGATATGGAGGGGGACTGGCAATGCAAATTTCTATTTTGCAAACGCTATGGCTTATGCTTGCTTCCAGATGATCTTTAGACCAATTTGTTATATGTTCAGTAATTCTCTGGGCCCACTACCTGTTTCCTTACAGATCGTTCTAGTGGTTGAGAGCGTAAGTACAATGCTGAATCACGACCGGAAACTGAGGAAGCTGTCTGCAGTGAAGCTTTCATGA

Protein sequence

MAAKEQFEEEEKKRKGNFWIWMVLSVIFRIVMIYFPNLNLSSRPEVSTPLTSIHRLAEGYWLKQSSMSPYAGFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQKDPELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLKVNLSKNSEFFPAGDIASLVYLWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATHLSLYPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNGFSLRPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFAEVFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYLSFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACFQMIFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVKLS
BLAST of Cp4.1LG05g03930 vs. Swiss-Prot
Match: PIGU_CRIGR (Phosphatidylinositol glycan anchor biosynthesis class U protein OS=Cricetulus griseus GN=PIGU PE=1 SV=3)

HSP 1 Score: 146.0 bits (367), Expect = 1.2e-33
Identity = 129/479 (26.93%), Postives = 211/479 (44.05%), Query Frame = 1

Query: 23  VLSVIFRIVMIYFPNLNLSSRPEVSTPLTSIHRLAEGYWLKQSSMSPYAGFLVFLSLSIQ 82
           V + +FR  +  F    +S R EV +PL+S  R+ EG  L    +SPY+G +        
Sbjct: 15  VRAALFRSSLAEF----ISERVEVVSPLSSWKRVVEGLSLLDLGVSPYSGAV-------- 74

Query: 83  MLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQKDPELASARSIMPKFD 142
                        ++   L+I+L    + +   V + +                     D
Sbjct: 75  -------------FHETPLIIYLFHFLIDYAELVFMIT---------------------D 134

Query: 143 VLSALLIRAVGQNLQRAYYRNLRLLK------------VNLSKNSEFFPAGDIASLVYLW 202
            L+A+ +    Q+  +  ++  +LL             +       + P     +L YL 
Sbjct: 135 ALTAIALYFAIQDFNKVVFKKQKLLLELDQYAPDVAELIRTPMEMRYIPLK--VALFYLL 194

Query: 203 NPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATHLSLYP 262
           NP+TI++CV  ST  I N  I   +    KG           V L+A    +AT+ +LYP
Sbjct: 195 NPYTILSCVAKSTCAINNTLIAFFILTTIKGS----------VFLSAIFLALATYQTLYP 254

Query: 263 IILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNGFSLRP 322
           +             L AP     LQR    V V+                     F +  
Sbjct: 255 V------------TLFAPGLLYLLQRQYIPVKVKSK------------------AFWIFS 314

Query: 323 VMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFAEV 382
             Y ++++    V + L   +        W+   + YGF+L+V DL+PNIG+ WY FAE+
Sbjct: 315 WEYAMMYIGSLVVIVCLSFFLLSS-----WDFIPAVYGFILSVPDLTPNIGLFWYFFAEM 374

Query: 383 FEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYLSF 442
           FE F  F++ VF IN+ F   PLA++L   P+F  F+ ++I ++ KSYP+VGD ALY++F
Sbjct: 375 FEHFSLFFVCVFQINVFFYTVPLAIKLKEHPIFFMFIQIAIISIFKSYPTVGDVALYMAF 400

Query: 443 MGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACFQMI 490
             ++ +    L   F L C  +  SLL PV+ +LWI+ G+ N+NF++A  + +   Q++
Sbjct: 435 FPVWNHLYRFLRNVFVLTCIIVVCSLLFPVLWHLWIYAGSANSNFFYAITLTFNVGQIL 400

BLAST of Cp4.1LG05g03930 vs. Swiss-Prot
Match: PIGU_RAT (Phosphatidylinositol glycan anchor biosynthesis class U protein OS=Rattus norvegicus GN=Pigu PE=1 SV=3)

HSP 1 Score: 143.7 bits (361), Expect = 6.0e-33
Identity = 137/467 (29.34%), Postives = 215/467 (46.04%), Query Frame = 1

Query: 23  VLSVIFRIVMIYFPNLNLSSRPEVSTPLTSIHRLAEGYWLKQSSMSPYAGFLVFLSLSIQ 82
           V + +FR  +  F    +S R EV +PL+S  R+ EG  L    +SPY+G +   +  I 
Sbjct: 15  VRAALFRSSLAEF----ISERVEVVSPLSSWKRVVEGLALLDLGVSPYSGAVFHETPLII 74

Query: 83  MLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQKDPELASARSIMPKFD 142
            L   L D       +  LV  ++      L+++ +     D  K   +   + ++ + D
Sbjct: 75  YLFHFLID-------YAELVFMITDA----LTAIALYFAIQDFNK--VVFKKQKLLLELD 134

Query: 143 VLSALLIRAVGQNLQRAYYRNLRLLKVNLSKNSEFFPAGDIASLVYLWNPFTIVACVGLS 202
             +  +   +   ++  Y      LKV               +L YL NP+TI++CV  S
Sbjct: 135 QYAPDVAELIRTPMEMRYIP----LKV---------------ALFYLLNPYTILSCVAKS 194

Query: 203 TSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATHLSLYPIILIIPVVLLLG 262
           T  I N  I   +    K          G V L+A    +AT+ SLYPI L  P +L L 
Sbjct: 195 TCAINNTLIAFFILTTIK----------GSVFLSAIFLALATYQSLYPITLFAPGLLYL- 254

Query: 263 NGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNGFSLRPVMYFLLWVSLFS 322
                      LQR    V V+      S+   + +            V+  L +  L S
Sbjct: 255 -----------LQRQYIPVKVK------SKAFWIFSWEYAMMYTGSLVVIVCLSFFLLSS 314

Query: 323 VYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFAEVFEFFRDFYLIVF 382
                            W+   + YGF+L+V DL+PNIG+ WY FAE+FE F  F++ VF
Sbjct: 315 -----------------WDFIPAVYGFILSVPDLTPNIGLFWYFFAEMFEHFSLFFVCVF 374

Query: 383 HINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYLSFMGLFVYELVDLE 442
            IN+ F   PLA++L   P+F  F+ ++I ++ KSYP+VGD ALY++F  ++ +    L 
Sbjct: 375 QINVFFYTVPLAIKLKEHPIFFMFIQIAIISIFKSYPTVGDVALYMAFFPVWSHLYRFLR 400

Query: 443 FSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACFQMI 490
             F L C  I  SLL PV+ +LWI+ G+ N+NF++A  + +   Q++
Sbjct: 435 NIFVLTCIIIVCSLLFPVLWHLWIYAGSANSNFFYAITLTFNVGQIL 400

BLAST of Cp4.1LG05g03930 vs. Swiss-Prot
Match: PIGU_HUMAN (Phosphatidylinositol glycan anchor biosynthesis class U protein OS=Homo sapiens GN=PIGU PE=1 SV=3)

HSP 1 Score: 142.9 bits (359), Expect = 1.0e-32
Identity = 136/467 (29.12%), Postives = 217/467 (46.47%), Query Frame = 1

Query: 23  VLSVIFRIVMIYFPNLNLSSRPEVSTPLTSIHRLAEGYWLKQSSMSPYAGFLVFLSLSIQ 82
           V + +FR  +  F    +S R EV +PL+S  R+ EG  L    +SPY+G +   +  I 
Sbjct: 15  VRAALFRSSLAEF----ISERVEVVSPLSSWKRVVEGLSLLDLGVSPYSGAVFHETPLII 74

Query: 83  MLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQKDPELASARSIMPKFD 142
            L   L D       +  LV  ++      L+++ +     D  K   +   + ++ + D
Sbjct: 75  YLFHFLID-------YAELVFMITDA----LTAIALYFAIQDFNK--VVFKKQKLLLELD 134

Query: 143 VLSALLIRAVGQNLQRAYYRNLRLLKVNLSKNSEFFPAGDIASLVYLWNPFTIVACVGLS 202
             +  +   +   ++  Y      LKV               +L YL NP+TI++CV  S
Sbjct: 135 QYAPDVAELIRTPMEMRYIP----LKV---------------ALFYLLNPYTILSCVAKS 194

Query: 203 TSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATHLSLYPIILIIPVVLLLG 262
           T  I N  I   +    KG      AF     L+A    +AT+ SLYP+ L +P +L L 
Sbjct: 195 TCAINNTLIAFFILTTIKGS-----AF-----LSAIFLALATYQSLYPLTLFVPGLLYL- 254

Query: 263 NGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNGFSLRPVMYFLLWVSLFS 322
                      LQR    V ++      S+   + +            V+  L +  L S
Sbjct: 255 -----------LQRQYIPVKMK------SKAFWIFSWEYAMMYVGSLVVIICLSFFLLSS 314

Query: 323 VYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFAEVFEFFRDFYLIVF 382
                            W+   + YGF+L+V DL+PNIG+ WY FAE+FE F  F++ VF
Sbjct: 315 -----------------WDFIPAVYGFILSVPDLTPNIGLFWYFFAEMFEHFSLFFVCVF 374

Query: 383 HINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYLSFMGLFVYELVDLE 442
            IN+ F   PLA++L   P+F  F+ +++ A+ KSYP+VGD ALY++F  ++ +    L 
Sbjct: 375 QINVFFYTIPLAIKLKEHPIFFMFIQIAVIAIFKSYPTVGDVALYMAFFPVWNHLYRFLR 400

Query: 443 FSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACFQMI 490
             F L C  I  SLL PV+ +LWI+ G+ N+NF++A  + +   Q++
Sbjct: 435 NIFVLTCIIIVCSLLFPVLWHLWIYAGSANSNFFYAITLTFNVGQIL 400

BLAST of Cp4.1LG05g03930 vs. Swiss-Prot
Match: PIGU_MOUSE (Phosphatidylinositol glycan anchor biosynthesis class U protein OS=Mus musculus GN=Pigu PE=1 SV=4)

HSP 1 Score: 141.7 bits (356), Expect = 2.3e-32
Identity = 136/467 (29.12%), Postives = 214/467 (45.82%), Query Frame = 1

Query: 23  VLSVIFRIVMIYFPNLNLSSRPEVSTPLTSIHRLAEGYWLKQSSMSPYAGFLVFLSLSIQ 82
           V + +FR  +  F    +S R EV +PL+S  R+ EG  L    +SPY+G +   +  I 
Sbjct: 15  VRAALFRSSLAEF----ISERVEVVSPLSSWKRVVEGLALLDLGVSPYSGAVFHETPLII 74

Query: 83  MLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQKDPELASARSIMPKFD 142
            L   L D       +  LV  ++      L+++ +     D  K   +   + ++ + D
Sbjct: 75  YLFHFLID-------YAELVFMITDA----LTAIALYFAIQDFNK--VVFKKQKLLLELD 134

Query: 143 VLSALLIRAVGQNLQRAYYRNLRLLKVNLSKNSEFFPAGDIASLVYLWNPFTIVACVGLS 202
             +  +   +   ++  Y      LKV L                YL NP+TI++CV  S
Sbjct: 135 QYAPDVAELIRTPMEMRYIP----LKVAL----------------YLLNPYTILSCVAKS 194

Query: 203 TSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATHLSLYPIILIIPVVLLLG 262
           T  I N  I   +    K          G V L+A    +AT+ SLYP+ L  P +L L 
Sbjct: 195 TCAINNTLIAFFILTTIK----------GSVFLSAVFLALATYQSLYPVTLFAPGLLYL- 254

Query: 263 NGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNGFSLRPVMYFLLWVSLFS 322
                      LQR    V V+      S+   + +            V+  L +  L S
Sbjct: 255 -----------LQRQYIPVKVK------SKAFWIFSWEYAMMYTGSLVVIVCLSFFLLSS 314

Query: 323 VYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFAEVFEFFRDFYLIVF 382
                            W+   + YGF+L+V DL+PNIG+ WY FAE+FE F  F++ VF
Sbjct: 315 -----------------WDFIPAVYGFILSVPDLTPNIGLFWYFFAEMFEHFSLFFVCVF 374

Query: 383 HINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYLSFMGLFVYELVDLE 442
            IN+ F   PLA++L   P+F  F+ ++I ++ KSYP+VGD ALY++F  ++ +    L 
Sbjct: 375 QINVFFYTVPLAIKLKEHPIFFMFIQIAIISIFKSYPTVGDVALYMAFFPVWNHLYRFLR 399

Query: 443 FSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACFQMI 490
             F L C  I  SLL PV+ +LWI+ G+ N+NF++A  + +   Q++
Sbjct: 435 NIFVLTCIIIVCSLLFPVLWHLWIYAGSANSNFFYAITLTFNVGQIL 399

BLAST of Cp4.1LG05g03930 vs. Swiss-Prot
Match: CDC91_YEAST (GPI transamidase component GAB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GAB1 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.9e-19
Identity = 53/167 (31.74%), Postives = 92/167 (55.09%), Query Frame = 1

Query: 318 VSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFAEVFEFFRDF 377
           VS+ S+ +LLL   S+      W      YG ++T + + PN+G+ WY F E+F+ F  F
Sbjct: 196 VSILSLLILLLFSYSILGSQS-WSFLTQVYGSIITFEKVFPNLGLWWYFFIEMFDTFIPF 255

Query: 378 YLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYLSFMGLFVYE 437
           +  VF+I I   ITP  +R H +P +   + +    + K YPS+GD+  + SF+  F   
Sbjct: 256 FKAVFNIFIAVFITPFTLRYHKQPFYAFILCIGWIVLTKPYPSLGDAGFFFSFLPFFTPL 315

Query: 438 LVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYA 485
              L +       ++   +L+P+ ++LW+  G+GN+NF++A ++ YA
Sbjct: 316 FGYLRYPIISALLFLHAIVLAPIFYHLWVVLGSGNSNFFYAISLVYA 361

BLAST of Cp4.1LG05g03930 vs. TrEMBL
Match: A0A0A0KP02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G155530 PE=4 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 8.3e-207
Identity = 402/533 (75.42%), Postives = 430/533 (80.68%), Query Frame = 1

Query: 4   KEQFEEEEKKRKGNFWIWMVLSVIFRIVMIYFPNLNLSSRPEVSTPLTSIHRLAEGYWLK 63
           KEQ E++ KK+KGNFWIWM+LSVIFR++MIYFPNLNLSSRPEV+T LTSIHRLAEGYWLK
Sbjct: 348 KEQIEDKMKKKKGNFWIWMMLSVIFRLLMIYFPNLNLSSRPEVATSLTSIHRLAEGYWLK 407

Query: 64  QSSMSPYAGFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRAT 123
           QSSMSPY G +   S     LLL L                        L  +TV  +  
Sbjct: 408 QSSMSPYTGSMYHGS----PLLLSL------------------------LGPLTV--KRI 467

Query: 124 DCQKDPELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLKVNLSKNSEFFPAGDI 183
           + Q D  L S   ++   DVLSALLIR  GQNLQRAYYR+L+LLKVNLSK+SE FPAGDI
Sbjct: 468 EGQPDHLLCSFAFVVA--DVLSALLIRGTGQNLQRAYYRSLKLLKVNLSKSSEIFPAGDI 527

Query: 184 ASLVYLWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVA 243
           ASLVY+WNPFTIVACVGLSTSPIENLAIVLTLYGASK          GQVPLAAFGFV+A
Sbjct: 528 ASLVYVWNPFTIVACVGLSTSPIENLAIVLTLYGASK----------GQVPLAAFGFVMA 587

Query: 244 THLSLYPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVP 303
           THLSLYP+ILIIPVVLLLGNGLDAPPRK F +RS SRVV +PS DSC Q EEVINQ +VP
Sbjct: 588 THLSLYPVILIIPVVLLLGNGLDAPPRKFFFERSCSRVVEQPSNDSCGQQEEVINQPKVP 647

Query: 304 NGFSLRPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVL 363
           NGFSLRPVMYFLLWVS+FS Y+LLLCG+SLKQFGGLWEMFRSTYGF+LTVQDLSPNIGVL
Sbjct: 648 NGFSLRPVMYFLLWVSVFSAYMLLLCGVSLKQFGGLWEMFRSTYGFILTVQDLSPNIGVL 707

Query: 364 WYLFAEVFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGD 423
           WYLFAEVFEFFRDFYLIVFHINILFMI PL++RL HRPLFLAFVLLSISAMLKSYPSVGD
Sbjct: 708 WYLFAEVFEFFRDFYLIVFHINILFMILPLSIRLCHRPLFLAFVLLSISAMLKSYPSVGD 767

Query: 424 SALYLSFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAY 483
           SALYLSFMGLFV  LVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAY
Sbjct: 768 SALYLSFMGLFVDVLVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAY 816

Query: 484 ACFQMIFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVKLS 537
           ACF                      QIVLVVESVSTMLNHDRKLRKLSAVKLS
Sbjct: 828 ACF----------------------QIVLVVESVSTMLNHDRKLRKLSAVKLS 816

BLAST of Cp4.1LG05g03930 vs. TrEMBL
Match: V4VLX6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019953mg PE=4 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 3.1e-153
Identity = 310/536 (57.84%), Postives = 375/536 (69.96%), Query Frame = 1

Query: 1   MAAKEQFEEEEKKRKGNFWIWMVLSVIFRIVMIYFP-NLNLSSRPEVSTPLTSIHRLAEG 60
           M  K++ ++ + K+  +FW+W+  SVIFR+++IYFP NLN SSRPEV+TP+TSI RLAEG
Sbjct: 1   METKKEEKKSKIKQTSSFWVWVTASVIFRLILIYFPKNLNFSSRPEVATPVTSIRRLAEG 60

Query: 61  YWLKQSSMSPYAGFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVS 120
           YWLKQSSMSPYAG +                       +    + LS I    +  +   
Sbjct: 61  YWLKQSSMSPYAGSM-----------------------YHGSPLLLSLIGPLTVKRIEGQ 120

Query: 121 SRATDCQKDPELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLK-VNLSKNSEFF 180
                C     LA   S M         LIRA GQ LQ +   +L+ L  V LSKNSE  
Sbjct: 121 PNHLLCSLLFLLADVVSAM---------LIRATGQTLQMSSCLSLKSLDLVELSKNSEML 180

Query: 181 PAGDIASLVYLWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAF 240
            +GDIA+LVYLWNPFTIVACVGLSTSPIENL ++L+LYGA           T   PLAAF
Sbjct: 181 SSGDIAALVYLWNPFTIVACVGLSTSPIENLVVILSLYGAC----------TRLPPLAAF 240

Query: 241 GFVVATHLSLYPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVIN 300
           G+V+ THLSLYP +LIIP++LLLGNG DAPPRKLFLQR  S+    PS DS  Q EE+ N
Sbjct: 241 GWVMGTHLSLYPGVLIIPLILLLGNGPDAPPRKLFLQRRCSKGGKNPSSDSSCQEEELFN 300

Query: 301 QSEVPNGFSLRPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSP 360
           QS++P  FS +PV++FL W S++SVY+L+LCGIS+KQ GG+ EMF+STYGF+LTV+DLSP
Sbjct: 301 QSKLPITFSWKPVIHFLFWASIWSVYVLVLCGISVKQNGGMLEMFKSTYGFILTVEDLSP 360

Query: 361 NIGVLWYLFAEVFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSY 420
           NIGVLWY FAEVFEFFR+F+LIVFH+NIL MI PLA+RL+HRP FLAF  ++IS+MLKSY
Sbjct: 361 NIGVLWYFFAEVFEFFRNFFLIVFHMNILVMILPLAIRLNHRPCFLAFAYIAISSMLKSY 420

Query: 421 PSVGDSALYLSFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFA 480
           PSVGDSALYL  +G FV+EL DL+FSFFLF G +G+SLLSPVMHNLWIWRGTGNANFY+A
Sbjct: 421 PSVGDSALYLGLLGWFVFELADLQFSFFLFWGCVGVSLLSPVMHNLWIWRGTGNANFYYA 472

Query: 481 NAMAYACFQMIFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVK 535
            AMA+ACF                      QIVLVVESVS +LN+DRKLRKLS  K
Sbjct: 481 TAMAFACF----------------------QIVLVVESVSAILNYDRKLRKLSITK 472

BLAST of Cp4.1LG05g03930 vs. TrEMBL
Match: A0A061E107_THECC (GPI transamidase subunit PIG-U isoform 4 OS=Theobroma cacao GN=TCM_007351 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 4.0e-153
Identity = 310/540 (57.41%), Postives = 380/540 (70.37%), Query Frame = 1

Query: 8   EEEEKKRKGNFWIWMVLSVIFRIVMIYFP-NLNLSSRPEVSTPLTSIHRLAEGYWLKQSS 67
           +++ K    +FW W++ S+IFR+++IYFP NLNL+SRPEVSTPLTS  RLAEGYWLKQ S
Sbjct: 6   KQKNKTASFSFWNWVIASIIFRLILIYFPKNLNLASRPEVSTPLTSFRRLAEGYWLKQLS 65

Query: 68  MSPYAGF---LVFLSLS--------IQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSS 127
           MSPYAGF     F SL         I +   K     +L + +   +   S + +  L  
Sbjct: 66  MSPYAGFSFCFFFFSLCPTFFNFFFILIRQKKEMYLIFLVFGYSGSMYHGSPLLLSLLGP 125

Query: 128 VTVSSRATDCQKDPELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLK-VNLSKN 187
           +TV  +  + Q +  L S  S++   DV+SA+LIRA G+NL+ AY R+L  L  V  +K 
Sbjct: 126 LTV--KRIEGQPNHLLYSLVSVIA--DVVSAILIRATGKNLRLAYLRSLESLDIVQQTKA 185

Query: 188 SEFFPAGDIASLVYLWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVP 247
           SE    GD A+L+YLWNPFTIVACVGLSTSPIEN+A++L LYGA           +  VP
Sbjct: 186 SEILSPGDAAALIYLWNPFTIVACVGLSTSPIENMAVILCLYGAC----------SRLVP 245

Query: 248 LAAFGFVVATHLSLYPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPE 307
           LAAFG+V+ATHLSLYP ILIIPV+ LLG G DAPPRKLFLQ+++               E
Sbjct: 246 LAAFGWVIATHLSLYPAILIIPVIFLLGCGPDAPPRKLFLQKNNK--------------E 305

Query: 308 EVINQSEVPNGFSLRPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQ 367
           EV+++S++  GFS RP+ +F  W  L+S Y+L+LCGISLKQFGGLWEMF+STYGF+LTV+
Sbjct: 306 EVLSKSKLRLGFSWRPITHFAFWALLWSAYVLVLCGISLKQFGGLWEMFKSTYGFILTVE 365

Query: 368 DLSPNIGVLWYLFAEVFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAM 427
           DLSPNIGVLWY FAEVF+FFR+F+LIVFH+NILFMI PLA+RLHHRP FLAFV ++I +M
Sbjct: 366 DLSPNIGVLWYFFAEVFDFFRNFFLIVFHVNILFMIMPLAIRLHHRPCFLAFVYIAIFSM 425

Query: 428 LKSYPSVGDSALYLSFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNAN 487
           LKSYPSVGDSALYL  +G FV EL D++FSFFLFCGY+G+SLLSPVMH LWIWRGTGNAN
Sbjct: 426 LKSYPSVGDSALYLGLLGWFVNELADIQFSFFLFCGYVGVSLLSPVMHTLWIWRGTGNAN 485

Query: 488 FYFANAMAYACFQMIFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVK 535
           FYFA AM YAC                      LQI+LVVE VS MLNHDRKLR L+  K
Sbjct: 486 FYFATAMVYAC----------------------LQIILVVEGVSAMLNHDRKLRNLATSK 495

BLAST of Cp4.1LG05g03930 vs. TrEMBL
Match: A0A061E8Q7_THECC (GPI transamidase subunit PIG-U isoform 2 OS=Theobroma cacao GN=TCM_007351 PE=4 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 7.6e-152
Identity = 309/544 (56.80%), Postives = 380/544 (69.85%), Query Frame = 1

Query: 8   EEEEKKRKGNFWIWMVLSVIFRIVMIYFP-NLNLSSRPEVSTPLTSIHRLAEGYWLKQSS 67
           +++ K    +FW W++ S+IFR+++IYFP NLNL+SRPEVSTPLTS  RLAEGYWLKQ S
Sbjct: 6   KQKNKTASFSFWNWVIASIIFRLILIYFPKNLNLASRPEVSTPLTSFRRLAEGYWLKQLS 65

Query: 68  MSPYAGF---LVFLSLS--------IQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSS 127
           MSPYAGF     F SL         I +   K     +L + +   +   S + +  L  
Sbjct: 66  MSPYAGFSFCFFFFSLCPTFFNFFFILIRQKKEMYLIFLVFGYSGSMYHGSPLLLSLLGP 125

Query: 128 VTVSSRATDCQKDPELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLKV--NLSK 187
           +TV  +  + Q +  L S  S++   DV+SA+LIRA G+NL+ AY R+L  L +      
Sbjct: 126 LTV--KRIEGQPNHLLYSLVSVIA--DVVSAILIRATGKNLRLAYLRSLESLDIVQQTKA 185

Query: 188 NSEFFPA---GDIASLVYLWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFT 247
           + EFF     GD A+L+YLWNPFTIVACVGLSTSPIEN+A++L LYGA           +
Sbjct: 186 SGEFFKILSPGDAAALIYLWNPFTIVACVGLSTSPIENMAVILCLYGAC----------S 245

Query: 248 GQVPLAAFGFVVATHLSLYPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSC 307
             VPLAAFG+V+ATHLSLYP ILIIPV+ LLG G DAPPRKLFLQ+++            
Sbjct: 246 RLVPLAAFGWVIATHLSLYPAILIIPVIFLLGCGPDAPPRKLFLQKNNK----------- 305

Query: 308 SQPEEVINQSEVPNGFSLRPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFL 367
              EEV+++S++  GFS RP+ +F  W  L+S Y+L+LCGISLKQFGGLWEMF+STYGF+
Sbjct: 306 ---EEVLSKSKLRLGFSWRPITHFAFWALLWSAYVLVLCGISLKQFGGLWEMFKSTYGFI 365

Query: 368 LTVQDLSPNIGVLWYLFAEVFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLS 427
           LTV+DLSPNIGVLWY FAEVF+FFR+F+LIVFH+NILFMI PLA+RLHHRP FLAFV ++
Sbjct: 366 LTVEDLSPNIGVLWYFFAEVFDFFRNFFLIVFHVNILFMIMPLAIRLHHRPCFLAFVYIA 425

Query: 428 ISAMLKSYPSVGDSALYLSFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGT 487
           I +MLKSYPSVGDSALYL  +G FV EL D++FSFFLFCGY+G+SLLSPVMH LWIWRGT
Sbjct: 426 IFSMLKSYPSVGDSALYLGLLGWFVNELADIQFSFFLFCGYVGVSLLSPVMHTLWIWRGT 485

Query: 488 GNANFYFANAMAYACFQMIFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKL 535
           GNANFYFA AM YAC                      LQI+LVVE VS MLNHDRKLR L
Sbjct: 486 GNANFYFATAMVYAC----------------------LQIILVVEGVSAMLNHDRKLRNL 499

BLAST of Cp4.1LG05g03930 vs. TrEMBL
Match: A0A061E0U1_THECC (GPI transamidase subunit PIG-U isoform 3 OS=Theobroma cacao GN=TCM_007351 PE=4 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.9e-151
Identity = 307/529 (58.03%), Postives = 371/529 (70.13%), Query Frame = 1

Query: 8   EEEEKKRKGNFWIWMVLSVIFRIVMIYFP-NLNLSSRPEVSTPLTSIHRLAEGYWLKQSS 67
           +++ K    +FW W++ S+IFR+++IYFP NLNL+SRPEVSTPLTS  RLAEGYWLKQ S
Sbjct: 6   KQKNKTASFSFWNWVIASIIFRLILIYFPKNLNLASRPEVSTPLTSFRRLAEGYWLKQLS 65

Query: 68  MSPYAGFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQ 127
           MSPYAG +   S     LLL L                        L  +TV  +  + Q
Sbjct: 66  MSPYAGSMYHGS----PLLLSL------------------------LGPLTV--KRIEGQ 125

Query: 128 KDPELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLK-VNLSKNSEFFPAGDIAS 187
            +  L S  S++   DV+SA+LIRA G+NL+ AY R+L  L  V  +K SE    GD A+
Sbjct: 126 PNHLLYSLVSVIA--DVVSAILIRATGKNLRLAYLRSLESLDIVQQTKASEILSPGDAAA 185

Query: 188 LVYLWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATH 247
           L+YLWNPFTIVACVGLSTSPIEN+A++L LYGA           +  VPLAAFG+V+ATH
Sbjct: 186 LIYLWNPFTIVACVGLSTSPIENMAVILCLYGAC----------SRLVPLAAFGWVIATH 245

Query: 248 LSLYPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNG 307
           LSLYP ILIIPV+ LLG G DAPPRKLFLQ+++               EEV+++S++  G
Sbjct: 246 LSLYPAILIIPVIFLLGCGPDAPPRKLFLQKNNK--------------EEVLSKSKLRLG 305

Query: 308 FSLRPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWY 367
           FS RP+ +F  W  L+S Y+L+LCGISLKQFGGLWEMF+STYGF+LTV+DLSPNIGVLWY
Sbjct: 306 FSWRPITHFAFWALLWSAYVLVLCGISLKQFGGLWEMFKSTYGFILTVEDLSPNIGVLWY 365

Query: 368 LFAEVFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSA 427
            FAEVF+FFR+F+LIVFH+NILFMI PLA+RLHHRP FLAFV ++I +MLKSYPSVGDSA
Sbjct: 366 FFAEVFDFFRNFFLIVFHVNILFMIMPLAIRLHHRPCFLAFVYIAIFSMLKSYPSVGDSA 425

Query: 428 LYLSFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYAC 487
           LYL  +G FV EL D++FSFFLFCGY+G+SLLSPVMH LWIWRGTGNANFYFA AM YAC
Sbjct: 426 LYLGLLGWFVNELADIQFSFFLFCGYVGVSLLSPVMHTLWIWRGTGNANFYFATAMVYAC 456

Query: 488 FQMIFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVK 535
                                 LQI+LVVE VS MLNHDRKLR L+  K
Sbjct: 486 ----------------------LQIILVVEGVSAMLNHDRKLRNLATSK 456

BLAST of Cp4.1LG05g03930 vs. TAIR10
Match: AT1G63110.1 (AT1G63110.1 GPI transamidase subunit PIG-U)

HSP 1 Score: 498.4 bits (1282), Expect = 5.4e-141
Identity = 285/524 (54.39%), Postives = 354/524 (67.56%), Query Frame = 1

Query: 8   EEEEKKRKGNFWIWMVLSVIFRIVMIYFP-NLNLSSRPEVSTPLTSIHRLAEGYWLKQSS 67
           EE++ K+   FWIW + S+ FR+++I FP NLNLSSRPEVSTPLTSI RLAEGYWLKQ+S
Sbjct: 3   EEQKMKQLSRFWIWALSSIAFRLILISFPGNLNLSSRPEVSTPLTSIRRLAEGYWLKQAS 62

Query: 68  MSPYAGFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQ 127
           MSPYAG +   S    +LL  L          +   +  S ++V                
Sbjct: 63  MSPYAGSMYHGS---PLLLSVLGPLTVQRIKGQPSHLLCSLVFV---------------- 122

Query: 128 KDPELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLK-VNLSKNSEFFPAGDIAS 187
               +A   S M         L+RA+GQ LQ AY  N RLL  +  S++    P GDIA+
Sbjct: 123 ----IADILSAM---------LLRAIGQKLQMAYGLNARLLGFLKSSRDKVILPCGDIAA 182

Query: 188 LVYLWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATH 247
           LVYLWNPFTIV+CVGLSTSPIENLA++L L+G          A T +VPLAAFG V+ATH
Sbjct: 183 LVYLWNPFTIVSCVGLSTSPIENLAVILALFG----------AVTRRVPLAAFGLVIATH 242

Query: 248 LSLYPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNG 307
           LSLYP  L IP++ LLG GLDAPP KLFLQ  S       S  + S+  ++   + +P  
Sbjct: 243 LSLYPATLTIPIIFLLGYGLDAPPIKLFLQTRSVE-NEESSTSTVSKQAKLKQTTHLP-- 302

Query: 308 FSLRPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWY 367
           F  + V +FL WV L+S+Y+L+LC +SL ++GGL EMF+ TYGF+L+++DLSPNIGV WY
Sbjct: 303 FLWKTVAHFLFWVLLWSLYVLILCALSLNKYGGLEEMFKRTYGFILSIEDLSPNIGVFWY 362

Query: 368 LFAEVFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSA 427
            FAEVF+FFR+F+LIV H+NILFM+ PLA+RL HRP FLAF+ L+IS++LKSYPSVGDSA
Sbjct: 363 FFAEVFDFFRNFFLIVLHVNILFMLLPLAIRLKHRPCFLAFIYLAISSILKSYPSVGDSA 422

Query: 428 LYLSFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYAC 487
           LYLS   LFV EL+D++FSFFLFCGY+GISLLSPVMHNLWIWRGTGNANFYF NA+ YAC
Sbjct: 423 LYLSLWALFVNELLDMKFSFFLFCGYLGISLLSPVMHNLWIWRGTGNANFYFGNAIGYAC 459

Query: 488 FQMIFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRK 530
           FQ++F                      VVESVS MLNHDR L++
Sbjct: 483 FQIVF----------------------VVESVSAMLNHDRALKR 459

BLAST of Cp4.1LG05g03930 vs. TAIR10
Match: AT1G12730.1 (AT1G12730.1 GPI transamidase subunit PIG-U)

HSP 1 Score: 461.8 bits (1187), Expect = 5.6e-130
Identity = 275/535 (51.40%), Postives = 349/535 (65.23%), Query Frame = 1

Query: 9   EEEKKRKG--------NFWIWMVLSVIFRIVMIYFP-NLNLSSRPEVSTPLTSIHRLAEG 68
           +EEKK  G         FWIW + S IFR+ +I+ P N+NLSSRPEVSTP TS+ RLAEG
Sbjct: 2   KEEKKDDGVMAEEKPYMFWIWTLASTIFRLFLIFSPANINLSSRPEVSTPHTSLRRLAEG 61

Query: 69  YWLKQSSMSPYAGFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVS 128
           YWLKQSS+SPYAG +   S     LLL +                        L  +TV 
Sbjct: 62  YWLKQSSLSPYAGSMYHGS----PLLLSI------------------------LGPLTV- 121

Query: 129 SRATDCQKDPELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLKV-NLSKNSEFF 188
            +  + Q    L S   ++   D+LSALL+RA+GQ LQ++Y  NLR L V   S++    
Sbjct: 122 -QRIEGQSSHHLCSLVFVIA--DLLSALLLRAIGQKLQKSYRMNLRPLGVLRSSQDRGIL 181

Query: 189 PAGDIASLVYLWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAF 248
           P GDIA+LVYLWNPFTI++CVGLSTSPIENLA++++LYGA           T +VPLAAF
Sbjct: 182 PCGDIAALVYLWNPFTILSCVGLSTSPIENLAVIVSLYGA----------VTRRVPLAAF 241

Query: 249 GFVVATHLSLYPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVIN 308
           G ++ATHLSLY   L+ P++ LLG GLDAPP K FLQ     V    + D  S+ E++  
Sbjct: 242 GLIMATHLSLYSAPLVFPLIFLLGYGLDAPPIKKFLQTRHGGVETGSTSD-VSKQEKLTP 301

Query: 309 QSEVPNGFSLRPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSP 368
            +++P  FS R V++F+ WV ++S Y+L+LC +SLKQ+GGL EMF+ TYGF+L ++DLSP
Sbjct: 302 TTQLP--FSWRTVLHFVFWVLVWSSYVLVLCSLSLKQYGGLEEMFKRTYGFILRIEDLSP 361

Query: 369 NIGVLWYLFAEVFEFFRDFYLIVFHINILFMITP-LAVRLHHRPLFLAFVLLSISAMLKS 428
           NIGV WY FAE FEF R++ LI+F++ IL    P L  RL HRP FLAF  L+ +++LKS
Sbjct: 362 NIGVFWYFFAEAFEFSRNYLLIIFNLYILLTSIPSLMFRLKHRPCFLAFAYLAFTSILKS 421

Query: 429 YPSVGDSALYLSFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYF 488
           YPSVGD+ALYLS   LFV EL D+E+SFF+FCGYIG SLLSPVMHN WIWRGTGNANFYF
Sbjct: 422 YPSVGDAALYLSLWALFVNELTDMEYSFFIFCGYIGFSLLSPVMHNFWIWRGTGNANFYF 469

Query: 489 ANAMAYACFQMIFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSA 533
            NAM YACFQ +F                      V +SV+ MLNHD+ L+K +A
Sbjct: 482 GNAMGYACFQFMF----------------------VEKSVNAMLNHDQALKKATA 469

BLAST of Cp4.1LG05g03930 vs. NCBI nr
Match: gi|659073716|ref|XP_008437214.1| (PREDICTED: phosphatidylinositol glycan anchor biosynthesis class U protein-like [Cucumis melo])

HSP 1 Score: 728.8 bits (1880), Expect = 6.9e-207
Identity = 401/525 (76.38%), Postives = 426/525 (81.14%), Query Frame = 1

Query: 12  KKRKGNFWIWMVLSVIFRIVMIYFPNLNLSSRPEVSTPLTSIHRLAEGYWLKQSSMSPYA 71
           KK+KGNFWIWM+LSVIFR++MIYFPNLNLSSRPEV+TPLTSIHRLAEGYWLKQSSMSPY 
Sbjct: 2   KKKKGNFWIWMMLSVIFRLLMIYFPNLNLSSRPEVATPLTSIHRLAEGYWLKQSSMSPYT 61

Query: 72  GFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQKDPEL 131
           G +   S     LLL L                        L  +TV  +    Q D  L
Sbjct: 62  GSMYHGS----PLLLSL------------------------LGPLTV--KRIKGQPDHLL 121

Query: 132 ASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLKVNLSKNSEFFPAGDIASLVYLWN 191
            S   ++   DVLSALLIR +GQNLQRAYY++L+LLKV LSKNSE FPAGDIASLVY+WN
Sbjct: 122 CSFAFVVA--DVLSALLIRGIGQNLQRAYYQSLKLLKVKLSKNSEIFPAGDIASLVYVWN 181

Query: 192 PFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATHLSLYPI 251
           PFTIVACVGLSTSPIENLAIVLTLYGASK          GQVPLAAFGFV+ATHLSLYP+
Sbjct: 182 PFTIVACVGLSTSPIENLAIVLTLYGASK----------GQVPLAAFGFVMATHLSLYPV 241

Query: 252 ILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNGFSLRPV 311
           ILIIPVVLLLGNGLDAPPRK F +R+ SRVV RPS DSCSQ EEVINQ EVPNGFSLRPV
Sbjct: 242 ILIIPVVLLLGNGLDAPPRKFFFERTRSRVVERPSNDSCSQQEEVINQPEVPNGFSLRPV 301

Query: 312 MYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFAEVF 371
           MYFLLWVSLFS YLLLLCG+SLKQFGGLWEMFRSTYGF+LTVQDLSPNIGVLWYLFAEVF
Sbjct: 302 MYFLLWVSLFSAYLLLLCGVSLKQFGGLWEMFRSTYGFILTVQDLSPNIGVLWYLFAEVF 361

Query: 372 EFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYLSFM 431
           EFFRDFYLIVFHINILFMI PL++RL HRPLFLAFVLLSISAMLKSYPSVGDSALYLSFM
Sbjct: 362 EFFRDFYLIVFHINILFMILPLSIRLCHRPLFLAFVLLSISAMLKSYPSVGDSALYLSFM 421

Query: 432 GLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACFQMIFR 491
           GLF+ ELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACF     
Sbjct: 422 GLFIDELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACF----- 462

Query: 492 PICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVKLS 537
                            QIVLVVESVSTMLNHDRKLRKLSA+KLS
Sbjct: 482 -----------------QIVLVVESVSTMLNHDRKLRKLSAMKLS 462

BLAST of Cp4.1LG05g03930 vs. NCBI nr
Match: gi|700194965|gb|KGN50142.1| (hypothetical protein Csa_5G155530 [Cucumis sativus])

HSP 1 Score: 728.0 bits (1878), Expect = 1.2e-206
Identity = 402/533 (75.42%), Postives = 430/533 (80.68%), Query Frame = 1

Query: 4   KEQFEEEEKKRKGNFWIWMVLSVIFRIVMIYFPNLNLSSRPEVSTPLTSIHRLAEGYWLK 63
           KEQ E++ KK+KGNFWIWM+LSVIFR++MIYFPNLNLSSRPEV+T LTSIHRLAEGYWLK
Sbjct: 348 KEQIEDKMKKKKGNFWIWMMLSVIFRLLMIYFPNLNLSSRPEVATSLTSIHRLAEGYWLK 407

Query: 64  QSSMSPYAGFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRAT 123
           QSSMSPY G +   S     LLL L                        L  +TV  +  
Sbjct: 408 QSSMSPYTGSMYHGS----PLLLSL------------------------LGPLTV--KRI 467

Query: 124 DCQKDPELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLKVNLSKNSEFFPAGDI 183
           + Q D  L S   ++   DVLSALLIR  GQNLQRAYYR+L+LLKVNLSK+SE FPAGDI
Sbjct: 468 EGQPDHLLCSFAFVVA--DVLSALLIRGTGQNLQRAYYRSLKLLKVNLSKSSEIFPAGDI 527

Query: 184 ASLVYLWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVA 243
           ASLVY+WNPFTIVACVGLSTSPIENLAIVLTLYGASK          GQVPLAAFGFV+A
Sbjct: 528 ASLVYVWNPFTIVACVGLSTSPIENLAIVLTLYGASK----------GQVPLAAFGFVMA 587

Query: 244 THLSLYPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVP 303
           THLSLYP+ILIIPVVLLLGNGLDAPPRK F +RS SRVV +PS DSC Q EEVINQ +VP
Sbjct: 588 THLSLYPVILIIPVVLLLGNGLDAPPRKFFFERSCSRVVEQPSNDSCGQQEEVINQPKVP 647

Query: 304 NGFSLRPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVL 363
           NGFSLRPVMYFLLWVS+FS Y+LLLCG+SLKQFGGLWEMFRSTYGF+LTVQDLSPNIGVL
Sbjct: 648 NGFSLRPVMYFLLWVSVFSAYMLLLCGVSLKQFGGLWEMFRSTYGFILTVQDLSPNIGVL 707

Query: 364 WYLFAEVFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGD 423
           WYLFAEVFEFFRDFYLIVFHINILFMI PL++RL HRPLFLAFVLLSISAMLKSYPSVGD
Sbjct: 708 WYLFAEVFEFFRDFYLIVFHINILFMILPLSIRLCHRPLFLAFVLLSISAMLKSYPSVGD 767

Query: 424 SALYLSFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAY 483
           SALYLSFMGLFV  LVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAY
Sbjct: 768 SALYLSFMGLFVDVLVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAY 816

Query: 484 ACFQMIFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVKLS 537
           ACF                      QIVLVVESVSTMLNHDRKLRKLSAVKLS
Sbjct: 828 ACF----------------------QIVLVVESVSTMLNHDRKLRKLSAVKLS 816

BLAST of Cp4.1LG05g03930 vs. NCBI nr
Match: gi|449452368|ref|XP_004143931.1| (PREDICTED: phosphatidylinositol glycan anchor biosynthesis class U protein [Cucumis sativus])

HSP 1 Score: 721.1 bits (1860), Expect = 1.4e-204
Identity = 398/525 (75.81%), Postives = 424/525 (80.76%), Query Frame = 1

Query: 12  KKRKGNFWIWMVLSVIFRIVMIYFPNLNLSSRPEVSTPLTSIHRLAEGYWLKQSSMSPYA 71
           KK+KGNFWIWM+LSVIFR++MIYFPNLNLSSRPEV+T LTSIHRLAEGYWLKQSSMSPY 
Sbjct: 2   KKKKGNFWIWMMLSVIFRLLMIYFPNLNLSSRPEVATSLTSIHRLAEGYWLKQSSMSPYT 61

Query: 72  GFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQKDPEL 131
           G +   S     LLL L                        L  +TV  +  + Q D  L
Sbjct: 62  GSMYHGS----PLLLSL------------------------LGPLTV--KRIEGQPDHLL 121

Query: 132 ASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLKVNLSKNSEFFPAGDIASLVYLWN 191
            S   ++   DVLSALLIR  GQNLQRAYYR+L+LLKVNLSK+SE FPAGDIASLVY+WN
Sbjct: 122 CSFAFVVA--DVLSALLIRGTGQNLQRAYYRSLKLLKVNLSKSSEIFPAGDIASLVYVWN 181

Query: 192 PFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATHLSLYPI 251
           PFTIVACVGLSTSPIENLAIVLTLYGASK          GQVPLAAFGFV+ATHLSLYP+
Sbjct: 182 PFTIVACVGLSTSPIENLAIVLTLYGASK----------GQVPLAAFGFVMATHLSLYPV 241

Query: 252 ILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNGFSLRPV 311
           ILIIPVVLLLGNGLDAPPRK F +RS SRVV +PS DSC Q EEVINQ +VPNGFSLRPV
Sbjct: 242 ILIIPVVLLLGNGLDAPPRKFFFERSCSRVVEQPSNDSCGQQEEVINQPKVPNGFSLRPV 301

Query: 312 MYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFAEVF 371
           MYFLLWVS+FS Y+LLLCG+SLKQFGGLWEMFRSTYGF+LTVQDLSPNIGVLWYLFAEVF
Sbjct: 302 MYFLLWVSVFSAYMLLLCGVSLKQFGGLWEMFRSTYGFILTVQDLSPNIGVLWYLFAEVF 361

Query: 372 EFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYLSFM 431
           EFFRDFYLIVFHINILFMI PL++RL HRPLFLAFVLLSISAMLKSYPSVGDSALYLSFM
Sbjct: 362 EFFRDFYLIVFHINILFMILPLSIRLCHRPLFLAFVLLSISAMLKSYPSVGDSALYLSFM 421

Query: 432 GLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACFQMIFR 491
           GLFV  LVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACF     
Sbjct: 422 GLFVDVLVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACF----- 462

Query: 492 PICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVKLS 537
                            QIVLVVESVSTMLNHDRKLRKLSAVKLS
Sbjct: 482 -----------------QIVLVVESVSTMLNHDRKLRKLSAVKLS 462

BLAST of Cp4.1LG05g03930 vs. NCBI nr
Match: gi|1009156506|ref|XP_015896278.1| (PREDICTED: phosphatidylinositol glycan anchor biosynthesis class U protein-like isoform X2 [Ziziphus jujuba])

HSP 1 Score: 554.3 bits (1427), Expect = 2.3e-154
Identity = 304/527 (57.69%), Postives = 381/527 (72.30%), Query Frame = 1

Query: 11  EKKRKGNFWIWMVLSVIFRIVMIYFP-NLNLSSRPEVSTPLTSIHRLAEGYWLKQSSMSP 70
           +KK++G+FWIW+  S+IFR+++IYFP NLNLSSRPEVSTPLTS+ RLAEGYWLKQSS+SP
Sbjct: 3   KKKKEGSFWIWVTASIIFRLILIYFPSNLNLSSRPEVSTPLTSLRRLAEGYWLKQSSVSP 62

Query: 71  YAGFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQKDP 130
           YAG +                     Y+   L++ +       L  +T+ S     +  P
Sbjct: 63  YAGSM---------------------YHGSPLLLAV-------LGPLTIKS----IEGQP 122

Query: 131 ELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLKVN-LSKNSEFFPAGDIASLVY 190
                  +    D ++ALLIRA+GQ LQ  Y R+L+ L ++ +SK+S   P+ DIA+L+Y
Sbjct: 123 NNLLCSLVFVIADFINALLIRAIGQKLQITYSRSLKSLDLDTVSKSSAVLPSRDIAALIY 182

Query: 191 LWNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATHLSL 250
           LWNPFTIVACVGLSTSPIENLAI+L++YGA           +  VPLAAFG+V+ATHLSL
Sbjct: 183 LWNPFTIVACVGLSTSPIENLAILLSIYGAC----------SRIVPLAAFGWVMATHLSL 242

Query: 251 YPIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNGFSL 310
           YP+ILI+PV+LLLG G D PPRKL LQR   ++   PS DS     E + + E+   FS 
Sbjct: 243 YPVILIVPVILLLGYGTDTPPRKLLLQRRGGKLEDNPSNDSDFHQGEAMKEPELAIVFSW 302

Query: 311 RPVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFA 370
           RPV  FLLW+ ++S+Y+L+LCGI +KQ+GGL EMF+STYGF+LT+QDLSPNIGVLWY FA
Sbjct: 303 RPVNLFLLWIIIWSLYVLVLCGIYVKQYGGLNEMFKSTYGFILTMQDLSPNIGVLWYFFA 362

Query: 371 EVFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYL 430
           EVF+FFR+F+LIVFH+NI+FMI PLA+RL+HRP FLAFV ++IS+MLKSYPSVGDSAL L
Sbjct: 363 EVFDFFRNFFLIVFHLNIIFMIFPLAIRLNHRPCFLAFVYITISSMLKSYPSVGDSALSL 422

Query: 431 SFMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACFQM 490
             +GLFV EL +++FSFF+FC Y+G+SLLSPVMHNLWIWRGTGNANFY+A AMAYAC   
Sbjct: 423 GLLGLFVNELAEMQFSFFIFCVYVGVSLLSPVMHNLWIWRGTGNANFYYATAMAYAC--- 465

Query: 491 IFRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVKL 536
                              LQI+LVVESVS MLNHDRKLRKLS+ KL
Sbjct: 483 -------------------LQIILVVESVSAMLNHDRKLRKLSSAKL 465

BLAST of Cp4.1LG05g03930 vs. NCBI nr
Match: gi|1009156508|ref|XP_015896279.1| (PREDICTED: phosphatidylinositol glycan anchor biosynthesis class U protein-like isoform X3 [Ziziphus jujuba])

HSP 1 Score: 553.9 bits (1426), Expect = 3.1e-154
Identity = 302/526 (57.41%), Postives = 377/526 (71.67%), Query Frame = 1

Query: 11  EKKRKGNFWIWMVLSVIFRIVMIYFP-NLNLSSRPEVSTPLTSIHRLAEGYWLKQSSMSP 70
           +KK++G+FWIW+  S+IFR+++IYFP NLNLSSRPEVSTPLTS+ RLAEGYWLKQSS+SP
Sbjct: 3   KKKKEGSFWIWVTASIIFRLILIYFPSNLNLSSRPEVSTPLTSLRRLAEGYWLKQSSVSP 62

Query: 71  YAGFLVFLSLSIQMLLLKLADCRWLSYNFENLVIFLSRIYVPWLSSVTVSSRATDCQKDP 130
           YAG +                     Y+   L++ +       L  +T+ S     +  P
Sbjct: 63  YAGSM---------------------YHGSPLLLAV-------LGPLTIKS----IEGQP 122

Query: 131 ELASARSIMPKFDVLSALLIRAVGQNLQRAYYRNLRLLKVNLSKNSEFFPAGDIASLVYL 190
                  +    D ++ALLIRA+GQ LQ  Y R+L+ L ++    S   P+ DIA+L+YL
Sbjct: 123 NNLLCSLVFVIADFINALLIRAIGQKLQITYSRSLKSLDLDTVSKSSVLPSRDIAALIYL 182

Query: 191 WNPFTIVACVGLSTSPIENLAIVLTLYGASKGKKLAIFAFTGQVPLAAFGFVVATHLSLY 250
           WNPFTIVACVGLSTSPIENLAI+L++YGA           +  VPLAAFG+V+ATHLSLY
Sbjct: 183 WNPFTIVACVGLSTSPIENLAILLSIYGAC----------SRIVPLAAFGWVMATHLSLY 242

Query: 251 PIILIIPVVLLLGNGLDAPPRKLFLQRSSSRVVVRPSRDSCSQPEEVINQSEVPNGFSLR 310
           P+ILI+PV+LLLG G D PPRKL LQR   ++   PS DS     E + + E+   FS R
Sbjct: 243 PVILIVPVILLLGYGTDTPPRKLLLQRRGGKLEDNPSNDSDFHQGEAMKEPELAIVFSWR 302

Query: 311 PVMYFLLWVSLFSVYLLLLCGISLKQFGGLWEMFRSTYGFLLTVQDLSPNIGVLWYLFAE 370
           PV  FLLW+ ++S+Y+L+LCGI +KQ+GGL EMF+STYGF+LT+QDLSPNIGVLWY FAE
Sbjct: 303 PVNLFLLWIIIWSLYVLVLCGIYVKQYGGLNEMFKSTYGFILTMQDLSPNIGVLWYFFAE 362

Query: 371 VFEFFRDFYLIVFHINILFMITPLAVRLHHRPLFLAFVLLSISAMLKSYPSVGDSALYLS 430
           VF+FFR+F+LIVFH+NI+FMI PLA+RL+HRP FLAFV ++IS+MLKSYPSVGDSAL L 
Sbjct: 363 VFDFFRNFFLIVFHLNIIFMIFPLAIRLNHRPCFLAFVYITISSMLKSYPSVGDSALSLG 422

Query: 431 FMGLFVYELVDLEFSFFLFCGYIGISLLSPVMHNLWIWRGTGNANFYFANAMAYACFQMI 490
            +GLFV EL +++FSFF+FC Y+G+SLLSPVMHNLWIWRGTGNANFY+A AMAYAC    
Sbjct: 423 LLGLFVNELAEMQFSFFIFCVYVGVSLLSPVMHNLWIWRGTGNANFYYATAMAYAC---- 464

Query: 491 FRPICYMFSNSLGPLPVSLQIVLVVESVSTMLNHDRKLRKLSAVKL 536
                             LQI+LVVESVS MLNHDRKLRKLS+ KL
Sbjct: 483 ------------------LQIILVVESVSAMLNHDRKLRKLSSAKL 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PIGU_CRIGR1.2e-3326.93Phosphatidylinositol glycan anchor biosynthesis class U protein OS=Cricetulus gr... [more]
PIGU_RAT6.0e-3329.34Phosphatidylinositol glycan anchor biosynthesis class U protein OS=Rattus norveg... [more]
PIGU_HUMAN1.0e-3229.12Phosphatidylinositol glycan anchor biosynthesis class U protein OS=Homo sapiens ... [more]
PIGU_MOUSE2.3e-3229.12Phosphatidylinositol glycan anchor biosynthesis class U protein OS=Mus musculus ... [more]
CDC91_YEAST2.9e-1931.74GPI transamidase component GAB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KP02_CUCSA8.3e-20775.42Uncharacterized protein OS=Cucumis sativus GN=Csa_5G155530 PE=4 SV=1[more]
V4VLX6_9ROSI3.1e-15357.84Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019953mg PE=4 SV=1[more]
A0A061E107_THECC4.0e-15357.41GPI transamidase subunit PIG-U isoform 4 OS=Theobroma cacao GN=TCM_007351 PE=4 S... [more]
A0A061E8Q7_THECC7.6e-15256.80GPI transamidase subunit PIG-U isoform 2 OS=Theobroma cacao GN=TCM_007351 PE=4 S... [more]
A0A061E0U1_THECC2.9e-15158.03GPI transamidase subunit PIG-U isoform 3 OS=Theobroma cacao GN=TCM_007351 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G63110.15.4e-14154.39 GPI transamidase subunit PIG-U[more]
AT1G12730.15.6e-13051.40 GPI transamidase subunit PIG-U[more]
Match NameE-valueIdentityDescription
gi|659073716|ref|XP_008437214.1|6.9e-20776.38PREDICTED: phosphatidylinositol glycan anchor biosynthesis class U protein-like ... [more]
gi|700194965|gb|KGN50142.1|1.2e-20675.42hypothetical protein Csa_5G155530 [Cucumis sativus][more]
gi|449452368|ref|XP_004143931.1|1.4e-20475.81PREDICTED: phosphatidylinositol glycan anchor biosynthesis class U protein [Cucu... [more]
gi|1009156506|ref|XP_015896278.1|2.3e-15457.69PREDICTED: phosphatidylinositol glycan anchor biosynthesis class U protein-like ... [more]
gi|1009156508|ref|XP_015896279.1|3.1e-15457.41PREDICTED: phosphatidylinositol glycan anchor biosynthesis class U protein-like ... [more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: Biological Process
TermDefinition
GO:0006506GPI anchor biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR009600PIG-U
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006506 GPI anchor biosynthetic process
biological_process GO:0016255 attachment of GPI anchor to protein
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0042765 GPI-anchor transamidase complex
molecular_function GO:0003674 molecular_function
molecular_function GO:0016788 hydrolase activity, acting on ester bonds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g03930.1Cp4.1LG05g03930.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009600GPI transamidase subunit PIG-UPANTHERPTHR13121GPI TRANSAMIDASE COMPONENT PIG-Ucoord: 109..487
score: 9.6E-132coord: 510..528
score: 9.6E-132coord: 1..76
score: 9.6E
IPR009600GPI transamidase subunit PIG-UPFAMPF06728PIG-Ucoord: 25..483
score: 1.8
NoneNo IPR availablePANTHERPTHR13121:SF0PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEINcoord: 510..528
score: 9.6E-132coord: 109..487
score: 9.6E-132coord: 1..76
score: 9.6E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG05g03930Cp4.1LG00g04770Cucurbita pepo (Zucchini)cpecpeB012