Cp4.1LG05g03440 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG05g03440
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Descriptionglutamate-cysteine ligase
LocationCp4.1LG05 : 1850273 .. 1856606 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTGGTTTCACAGACTGGTTCGGCTCACCGGATTGGTGTAGAGAGTGTACGACGGAGTGGAGGATTTATGGCTTTCAAGTTGTGCACTCATATGGAGTCGTTGAAGATGAAGGAGAATCTTATTAGTGCGTCTTCTCTGTCGTACAGTTCTGGTAATCTCGCGGATGGTTTGCGTATAGACAGCGCGAGGATGAGGTCGAAGCGTGGAGGTAGGGTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCTACCCAACCGTTGACGAAAGAGGATCTCGTGGCTTATTTAGCTTCTGGATGCAAGCCAAAGGAAAAGTGGAGGTTAATATTTCATACGCTTTCCCGTATTTAAATCAGAAAACTAACTTCCCTCAGTGTGCTTTTGGTTGTTATTCATCATTTACTAATAACAAATTTGTTCAACTTATTCATGCCAGTCACTGTAATCAGATTGTATGCCCTATACATACATACATACATGTATATATATACACTCGCACACACCTTTTGTTATTTCTTCACTTTTGCTCGGATTTTGGCAAGTTTACTGGATAATTTTTTTCATCTTTTTATATCTTATTCTTTTCAAGAAATATTTTAATGAGTGTTCTTTATTCTTTTATTATAATTTCTATTAAAAATTAAAATAACATAGCAATAGATCTAATAATATTTTAACTTAAAATTTAATACTAAATAAATAAATATATATATATATATATATATATATATATATATATATATATNGCTTTGGGATTCTCAAAAGACCTCAAACCAATGGAGAGAGTATTATTTGATTATAAACTCACGAACATTCCATAAATTAATCGATGTGGGACTTTCATCATCCAATATATACAATACAACGTATATACACATGAAAACTGCATGTATGTGGAGCAGAGTAAAACCGTACACTCAACCGCACCGTATCCGGCCTTTTCCTTCCACTATATTAGGCCGTTTTTTTCCCTCCGTTTCTCAAATCCATCGCTCGCTTGCCAAATCTCCTACAAAAAGGAAAAGCGAAGAGCGCCGGAGGGTTCTACGCTTCCAGGTAATTGTTTGATGATGAAATTCCGAAAACAACTCCATGTGTCCGATCCATTTTTCTGTTCTGCAGTTGATTTTTCTTTCAGTATTCGACCTGTTGTTGTAGTATGGATTTGTTTTTACTGTATTTTGCTGTTTTGTCATGGTTTTCTTCGGAATAGAGTGTAACTGAGCGGTTGCCTACTGTTTAGAATCGCGCAATGGCTCTGGTTTCACAGACTGGTTCGGCTCACCGGATTGGTGTAGAGAGTGTACGACGGAGTGGAGGATTTATGGCTTTCAAGTTGTGCACTCATATGGAGTCGTTGAAGATGAAGGAGAATCTTATTAGTGCGTCTTCTCTGTCGTACAGTTCTGGTAATCTCGCGGATGGTTTGCGTATAGACAGCGCGAGGATGAGGTCGAAGCGTGGAGGTAGGGTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCNGGACAGCGCGAGGATGAGGTCGAAGCGTGGAGGTAGGTTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCTACCCAACCGTTGACGAAAGAGGATCTCGTGGCTTATTTAGCTTCTGGATGCAAGCCAAAGGAAAAGTGGAGGTTAATATTTCATACGCTTTCCCGTATTTAAATCAGAAAACTAACTTCCCTCAGTGTGCTTTTGGTTGTTATTCATCATTTACTAATAACAAATTTGTTCAACTTATTCATGCCAGTCACTGTAATCAGATTGTATGCCCTATACATACATACATACATGTATATATATACACTCGCACACACCTTTTGTTATTTCTTCACTTTTNATGCTTTTGGTTGTTATTCATCATTTACTAATAACAAATTTGTTCAACTTATTCATGCCAGTCACTGTAATCGGATTGTATGCGCTATACATACATACATACATACATGTATATATACACTCGCACACACCTTTTGTTATTTCTTCACTTTTGCTCGGATTTTGGCAAGTTTACTGGATAATTTTTTTCATCTTTTTATATCTTATTCTTTTCAAGAAATATTTTAATGAGTGTTCTTTATTCTTTTATTATAATTTCTATTAAAAATTAAAATAACATAGTAATAGATCTAATAATATTTTAACTTAAAATTTAATACTAAATAAATAAATATAATATATATATATATATTTTGGTCTTAATATGTGCGTGTGTGTAAAGTGATGGGGCCAGGAATTTGGCTGGNATTATAATTTCTATTAAAAATTAAAATAACATAGCAATAGATCTAATAATATTTTAACTTAAAATTTAATACTAAATAAATAAATATATATATATATATATATATATATATAATCTTAATATGTGCGTGTGTGTAAAGTGATGGGGCCAGGAATTTGGCTGGACTGGAGACAAGGACGCGAAATGCAATTCCGAGAAATCGCTCCTTGCTCTTTTACTCATTATAATCTGCAGCTTTTGAAAACACTATCAATTTTTTTATTTTTTTTTATTTTAACAATTTGATCCTGGGTCCCCGGCAGAATTGGCACAGAACATGAGAAGTTTGGATTCCAGATTGGAAGTTTACGTCCCATGACGTACGAGCAAATAAGTGGGTTGCTAAACGGGCTTGCAGAGAGGTTTGGTTGGGAAAAAGTAATGGAAGGCAGCGACATTATAGGGCTCCGGCAGGTAAACCGCCCAGACCCTGCCAGTAAATACTGGCGAAATAGCAACGTAGCACCTATGCACTGCGTCTGACCTGTTTATAAGTAGATGCTCTGTTGGTATCCTTCCAGAAGAAATGTTTCCATTTTCTTCCTAACACGAGATTATTATGGACCAGGGTATGCAAGGTATATCACTGGAACCTGGGGGTCAATTTGAACTCAGTGGTGCCCCTCTTCAAACTTTGCATCAAACCTGTGCTGAAGTTAATTCACACCTTTACCAGGTTTGTGCCACGTTGCTCCTGTATGGCGTGCACACATTTTCTGTTCATTTTCAATGCCTATCCTCCCTTTTATTTTTTTTTTCAAAAGTTCCAACACCGTTTTGAGAGATTTCTCACAGTTAATTATGGGAACCTGAGAGAATAACCCCTAAAATTATATTCCCTGCCCTANATTTTTTCAAAAGTTCCAACACCGTTTTGAGAGATTTCTCACAGTTAGTTATGGAACCTGAGAGAATAACCCCTAAAATTATATTCCCTGCCCTACGTTATTTTATATATATCTTAATTTTCTTTTACTTAGTAAATATATATTGTAATATTTAGATTCCATTTTTTTAGAAATTATGTTAAATATTACATTTACTTTTTGTTTATGAAAAGTTATTAAAATTTTAAATATGAATTATATTAAAAAAAATAAAAATTATTCAAAACGAATGATATTTTAAAAGAAAATAAATTAAAAATGAGAAAAATGTATGTGAAAAATCTCGTAGAATAAATGAATAATTTCACCAACATAAGAAGAATCAAAATAGAAGACGTCACATCTCCAGTTTTAATTGTAAATTATCTGGTTGACTTCTGTAGGCGAAAGCTGTAGCTGAGGAGATGGGTATTGGATTTTTAGGAATTGGATTCCAGCCCAAATGGAGACGTGAAGACATACCTATGATGCCCAAGGTATACCTCTCTCTCTCTCTCTCTCTATATATATATATATATGTTTAGCACGTTTTACACGAATTATTATTTGGTGTCAGGGACGATACAAGATTATAGAGAATTACATGCCCAAAGTTNATTTGGTCTCAGGGACGATACAAGATTATAGAGAATTACATGCCCAAAGTTGGTTCACTTGGGCTGGACATGATGCTTAGGACATGCACCGTGCAGGTTAATCTAGATTTACGTCCTCTAAATGAGGTCAATGAGTCTACTTTTTCTAAATCAGATTAATTGTCTAATAATTCATGNTAGGTCAATAAGTCTACTTTTTCTAAATCAGATTAATTGTCTAATAATTCATGTAGGTTAATCTAGATTTTGATTCCGAAGCCGACATGATCAGAAAGTTCCGAGTGGGTCTTGCTCTTCAACCTGTGAGGAACCTTGTCTTTTTAAATATGAATTATGTTTTAAAAGCTACGTCTGTTCAATGTGTCTGAATACTTGTATAAGTGAACAGATAGCAACTGCTCTGTTTGCCAATTCACCATTTACCGAAGGAAAGTCAAATGGTTATTTGAGCATGAGAAGGTATGCTCTTGTTGGTTTTCATATAAATTTTTTTCTCTGTTTTTCTCCCTCCCTACCACATTAATCCATGCTCATGTTGCTTTTCAATTGAATTTCTGTATTAGAACAGCCAAATATGGACCGATACTGATAATAGCCGCACTGGTATGCTTCCATTTGTATTCGATGACTCCTTCGGGTAATCTGCCTCTTCCGGAACCGAGCTTTTTCTTTGCAGTGCTTTTCTTTATAAATATTTCCTGGGATTTCTTCTTCCTTAATGTATGTCGATGATTAAATTCAGTATTTTCTTTTATAATCTTCTTATGACGTTCTAGAGATTATTGTAACTTGGTGGCTCATAGCATGCATCATAACTGAAGAATCAAGCTAGGGTGCCCTAGCCTTTGCAACTTCTTGTATTTTTTTTTTTTTTTTTTTTTTNATGCAGATTTGAGAAGTATGTTGATTATGCTCTTGATGTTCCAATGTATTTCATATATCGGAAAAAGAAGTTTATTGACTGTTCTGGAATGTCCTTCAGGGTATGCATTTACCCATCATAATCGTCCAATTGCGAAGCTTTCTGTCTATCTCTTCCTTTCTATTGATCTCAATTTTTATTTTTAAGTTATTGACAATCGGTACATTATTGGGTTGTCTTTCCACAGGACTTCATGGCAGGAAAGCTCTCTCCAGTTCCTGGAGAATTTCCAACACTTTCTGATTGGGAAAATCACTTGACTACAATATATCCAGAGGTATCCTATGATCAATTTGCTCCAATATTGTGCTTGTTATGACCTAATTATAGGATAAAATTTTCCCATATTACTCAGGTCAGGTTGAAGAGATATTTGGAGATGAGGGGTGGTGATGGGGGCCCTTGGAGGAGAATATGTGCTCTGCCAGCATTTTGGGTAATCTTCATGATATAATCAAGCCATGTAGACCTTTTCTTAGACATGGTTGTAATATGTATTCTAAAAACTAAGAAGCTTGCTTAAAATTTTGGAGTTGGTTCTTTCTGTCTGGGGGGGCGGTTCATTATTACTTGATCTATAACTATTCTATGTATAGAATCAGGTGTTTAGAGTACTGCTAATGCTCTCTCTTCAAATGTTAGGTTGGTTTATTATATGACGAAGTTTCCCTGCAAAATGCTTTAGACATAACAGCTGACTGGACACCTGAAGAAAGACAGATGTTGAGGAATGAGGTAATAGGATTTTAGGGGAAACATTTTTTTTCTTCTAGTTGCCCATTGCTACTTGTGAGAAGCAGGGCAGACTCCGGCTCCTTCATTTGAACTAGGTTATGAAATTTAAATGTGTTGCAATAAATTCTACATGCCTTAATACTGAATAATTTTTTATCAGGTTCCAATAACCGGTCTCAACACACCATTTCGTGATGGGTTGCTGAAGCACGTTGCTCAAGATGTTGTTGAGTTAGCAAAGGTATGTCTAGCAATATTATTATCTCTTGTGAGTGCTGGTCTTATTATTGGCACCACTAATGTTGAGACTGGAACAAAAACTTCCCCACTTTCAGGATGGCTTAAAGAGAAGAGGTTTCAAGGAGTCTGGCTTCTTGAATGAGGTGACTGAAGTAGCTAGAACAGGTATGAACAACTTTTATGTTAATTTTTTGCACAAAAAGTATTGAACTCTCTCATTTTCCTTATTTCCTTTCTTTAAGAAACCTGAGTTCTATCTTTCAATTGGCTGAATTCCTGTAAGAAACCTGAATTCCTTGTTTTGCTTACATATTTGGAGCCCTAAAAAAACACTGCATGGGTTTAATTTAATTACAGGTGTGACACTGGCTGAAAAGATGTTAGGATTGTATAATGGAAAATGGGGACAAAATGTAGATCCAGTTTTCGAGGAGCTTCTATATTAAGCTGCATCTCTTTTAGTTATTGAGATTGTTACATGAACAACATAACTACACGTTCTTGAAACTTGTCCTTAGCTTGATGATGGTATTCATAGTTGTAGTAATTCTGTGATGGCCCTCTTTTGATTGTGGTGAATTGTAACCCTACAGCTTACCGAGCCATGAATCTTCTGTTCA

mRNA sequence

ATGGCTCTGACTGGTTCGGCTCACCGGATTGGTGTAGAGAGTGTACGACGGAGTGGAGGATTTATGGCTTTCAAGTTGTGCACTCATATGGAGTCGTTGAAGATGAAGGAGAATCTTATTAGTGCGTCTTCTCTGTCGTACAGTTCTGGTAATCTCGCGGATGGTTTGCGTATAGACAGCGCGAGGATGAGGTCGAAGCGTGGAGGTAGGGTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCTACCCAACCGTTGACGAAAGAGGATCTCGTGGCTTATTTAGCTTCTGGATGCAAGCCAAAGGAAAAGTGGAGTTCTGGTAATCTCGCGGATGGTTTGCGTATAGACAGCGCGAGGATGAGGTCGAAGCGTGGAGGTAGGGTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCNGGACAGCGCGAGGATGAGGTCGAAGCGTGGAGGTTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCTACCCAACCGTTGACGAAAGAGGATCTCGTGGCTTATTTAGCTTCTGGATGCAAGCCAAAGGAAAAGTGGAGAATTGGCACAGAACATGAGAAGTTTGGATTCCAGATTGGAAGTTTACGTCCCATGACGTACGAGCAAATAAGTGGGTTGCTAAACGGGCTTGCAGAGAGGTTTGGTTGGGAAAAAGTAATGGAAGGCAGCGACATTATAGGGCTCCGGCAGGGTATGCAAGGTATATCACTGGAACCTGGGGGTCAATTTGAACTCAGTGGTGCCCCTCTTCAAACTTTGCATCAAACCTGTGCTGAAGTTAATTCACACCTTTACCAGGGACGATACAAGATTATAGAGAATTACATGCCCAAAGTTGGTTCACTTGGGCTGGACATGATGCTTAGGACATGCACCGTGCAGGTTAATCTAGATTTTGATTCCGAAGCCGACATGATCAGAAAGTTCCGAGTGGGTCTTGCTCTTCAACCTATAGCAACTGCTCTGTTTGCCAATTCACCATTTACCGAAGGAAAGTCAAATGGTTATTTGAGCATGAGAAGCCAAATATGGACCGATACTGATAATAGCCGCACTGGTATGCTTCCATTTGTATTCGATGACTCCTTCGGATTTGAGAAGTATGTTGATTATGCTCTTGATGTTCCAATGTATTTCATATATCGGAAAAAGAAGTTTATTGACTGTTCTGGAATGTCCTTCAGGGACTTCATGGCAGGAAAGCTCTCTCCAGTTCCTGGAGAATTTCCAACACTTTCTGATTGGGAAAATCACTTGACTACAATATATCCAGAGGTCAGGTTGAAGAGATATTTGGAGATGAGGGGTGGTGATGGGGGCCCTTGGAGGAGAATATGTGCTCTGCCAGCATTTTGGGTTGGTTTATTATATGACGAAGTTTCCCTGCAAAATGCTTTAGACATAACAGCTGACTGGACACCTGAAGAAAGACAGATGTTGAGGAATGAGGTTCCAATAACCGGTCTCAACACACCATTTCGTGATGGGTTGCTGAAGCACGTTGCTCAAGATGTTGTTGAGTTAGCAAAGGATGGCTTAAAGAGAAGAGGTTTCAAGGAGTCTGGCTTCTTGAATGAGGTGACTGAAGTAGCTAGAACAGGTGTGACACTGGCTGAAAAGATGTTAGGATTGTATAATGGAAAATGGGGACAAAATGTAGATCCAGTTTTCGAGGAGCTTCTATATTAAGCTGCATCTCTTTTAGTTATTGAGATTGTTACATGAACAACATAACTACACGTTCTTGAAACTTGTCCTTAGCTTGATGATGGTATTCATAGTTGTAGTAATTCTGTGATGGCCCTCTTTTGATTGTGGTGAATTGTAACCCTACAGCTTACCGAGCCATGAATCTTCTGTTCA

Coding sequence (CDS)

ATGGCTCTGACTGGTTCGGCTCACCGGATTGGTGTAGAGAGTGTACGACGGAGTGGAGGATTTATGGCTTTCAAGTTGTGCACTCATATGGAGTCGTTGAAGATGAAGGAGAATCTTATTAGTGCGTCTTCTCTGTCGTACAGTTCTGGTAATCTCGCGGATGGTTTGCGTATAGACAGCGCGAGGATGAGGTCGAAGCGTGGAGGTAGGGTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCTACCCAACCGTTGACGAAAGAGGATCTCGTGGCTTATTTAGCTTCTGGATGCAAGCCAAAGGAAAAGTGGAGTTCTGGTAATCTCGCGGATGGTTTGCGTATAGACAGCGCGAGGATGAGGTCGAAGCGTGGAGGTAGGGTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCNGGACAGCGCGAGGATGAGGTCGAAGCGTGGAGGTTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCTACCCAACCGTTGACGAAAGAGGATCTCGTGGCTTATTTAGCTTCTGGATGCAAGCCAAAGGAAAAGTGGAGAATTGGCACAGAACATGAGAAGTTTGGATTCCAGATTGGAAGTTTACGTCCCATGACGTACGAGCAAATAAGTGGGTTGCTAAACGGGCTTGCAGAGAGGTTTGGTTGGGAAAAAGTAATGGAAGGCAGCGACATTATAGGGCTCCGGCAGGGTATGCAAGGTATATCACTGGAACCTGGGGGTCAATTTGAACTCAGTGGTGCCCCTCTTCAAACTTTGCATCAAACCTGTGCTGAAGTTAATTCACACCTTTACCAGGGACGATACAAGATTATAGAGAATTACATGCCCAAAGTTGGTTCACTTGGGCTGGACATGATGCTTAGGACATGCACCGTGCAGGTTAATCTAGATTTTGATTCCGAAGCCGACATGATCAGAAAGTTCCGAGTGGGTCTTGCTCTTCAACCTATAGCAACTGCTCTGTTTGCCAATTCACCATTTACCGAAGGAAAGTCAAATGGTTATTTGAGCATGAGAAGCCAAATATGGACCGATACTGATAATAGCCGCACTGGTATGCTTCCATTTGTATTCGATGACTCCTTCGGATTTGAGAAGTATGTTGATTATGCTCTTGATGTTCCAATGTATTTCATATATCGGAAAAAGAAGTTTATTGACTGTTCTGGAATGTCCTTCAGGGACTTCATGGCAGGAAAGCTCTCTCCAGTTCCTGGAGAATTTCCAACACTTTCTGATTGGGAAAATCACTTGACTACAATATATCCAGAGGTCAGGTTGAAGAGATATTTGGAGATGAGGGGTGGTGATGGGGGCCCTTGGAGGAGAATATGTGCTCTGCCAGCATTTTGGGTTGGTTTATTATATGACGAAGTTTCCCTGCAAAATGCTTTAGACATAACAGCTGACTGGACACCTGAAGAAAGACAGATGTTGAGGAATGAGGTTCCAATAACCGGTCTCAACACACCATTTCGTGATGGGTTGCTGAAGCACGTTGCTCAAGATGTTGTTGAGTTAGCAAAGGATGGCTTAAAGAGAAGAGGTTTCAAGGAGTCTGGCTTCTTGAATGAGGTGACTGAAGTAGCTAGAACAGGTGTGACACTGGCTGAAAAGATGTTAGGATTGTATAATGGAAAATGGGGACAAAATGTAGATCCAGTTTTCGAGGAGCTTCTATATTAA

Protein sequence

MALTGSAHRIGVESVRRSGGFMAFKLCTHMESLKMKENLISASSLSYSSGNLADGLRIDSARMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWSSGNLADGLRIDSARMRSKRGGRVIVATGAPAEEATVAGQREDEVEAWRLIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQGRYKIIENYMPKVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKMLGLYNGKWGQNVDPVFEELLY
Homology
[Close]
BLAST of Cp4.1LG05g03440 vs. Swiss-Prot
Match: GSH1_TOBAC (Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum GN=GSH1 PE=2 SV=2)

HSP 1 Score: 716.1 bits (1847), Expect = 3.1e-205
Identity = 343/451 (76.05%), Postives = 380/451 (84.26%), Query Frame = 1 

Query: 159 IVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQ 218
           IVA   P E+A VA +PLTKEDLVAYLASGCK KEKWRIGTEHEKFGF+ G+LRPM YEQ
Sbjct: 72  IVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYEQ 131

Query: 219 ISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNS 278
           I+ LLNG+AERF WEKVMEG +IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNS
Sbjct: 132 IAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNS 191

Query: 279 HLYQGRYKIIE--------NYMPKVGSL-----------------------GLDMMLRTC 338
           HLYQ +    E         + PK G                         GLDMM RTC
Sbjct: 192 HLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTC 251

Query: 339 TVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSR 398
           TVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGK NGYLSMRS IWTDTDN+R
Sbjct: 252 TVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNR 311

Query: 399 TGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPT 458
            GMLPFVFDDSFGFE+YVDYALDVPMYF+YRKKK+IDC+GMSFRDFM GKLSP+PG++PT
Sbjct: 312 AGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFMNGKLSPIPGDYPT 371

Query: 459 LSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITAD 518
           L+DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVG+LYDEVSLQ  LD+T+D
Sbjct: 372 LNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQTVLDMTSD 431

Query: 519 WTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVA 578
           WT EER+MLRN+VP +GL TPFRDGLLKHVAQDVV+LAK+GL+RRG+KE+GFLNEVTEV 
Sbjct: 432 WTAEEREMLRNKVPTSGLKTPFRDGLLKHVAQDVVKLAKEGLERRGYKETGFLNEVTEVV 491

[Close]
BLAST of Cp4.1LG05g03440 vs. Swiss-Prot
Match: GSH1_SOLLC (Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum GN=GSH1 PE=2 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 1.8e-200
Identity = 335/451 (74.28%), Postives = 372/451 (82.48%), Query Frame = 1 

Query: 159 IVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQ 218
           IVA   P E+A VA +PLTKEDLV YLASGCK KEKWRIGTEHEKFGF+ G+LRPM Y+Q
Sbjct: 73  IVAASPPTEDAVVAAEPLTKEDLVGYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYDQ 132

Query: 219 ISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNS 278
           I+ LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNS
Sbjct: 133 IADLLNGIAERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNS 192

Query: 279 HLYQGRYKIIE--------NYMPKVGSL-----------------------GLDMMLRTC 338
           HLYQ +    E         + PK G                         GLDMM RTC
Sbjct: 193 HLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPIMPKGRYEIIRNYMPKVGSLGLDMMFRTC 252

Query: 339 TVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSR 398
           TVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGK NGYLS RS IWTDTDN+R
Sbjct: 253 TVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSKRSHIWTDTDNNR 312

Query: 399 TGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPT 458
            GMLPFVFDDSFGFE+YVDYALDVPMYF+YRKKK++DC+G+SFRDFM GKL P+PGE+PT
Sbjct: 313 AGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYVDCTGLSFRDFMNGKLPPIPGEYPT 372

Query: 459 LSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITAD 518
           L+DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVG+LYDE SLQ+ LD+T D
Sbjct: 373 LNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEGSLQSVLDMTFD 432

Query: 519 WTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVA 578
           WT EER MLRN+VP +GL TPFRDGLL HVAQDVV+LAK+GL+RRGFKE+GFLNEV EV 
Sbjct: 433 WTAEERDMLRNKVPKSGLKTPFRDGLLMHVAQDVVKLAKEGLERRGFKETGFLNEVAEVV 492

[Close]
BLAST of Cp4.1LG05g03440 vs. Swiss-Prot
Match: GSH1_MEDTR (Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula GN=GSH1 PE=2 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 1.7e-198
Identity = 335/457 (73.30%), Postives = 376/457 (82.28%), Query Frame = 1 

Query: 157 RLIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTY 216
           R IVA   P E+A VAT+PLTK+DL+ YLASGCK K+KWRIGTEHEKFGF++GSLRPM Y
Sbjct: 56  RFIVAASPPTEDAVVATEPLTKQDLIDYLASGCKTKDKWRIGTEHEKFGFELGSLRPMKY 115

Query: 217 EQISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEV 276
           EQIS LLNG+AERF W+KVMEG +IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEV
Sbjct: 116 EQISELLNGIAERFDWDKVMEGDNIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEV 175

Query: 277 NSHLYQ-------------------------------GRYKIIENYMPKVGSL----GLD 336
           NSHLYQ                               GRY+I    M K        GLD
Sbjct: 176 NSHLYQVKAVAEEMGIGFLGIGFQPKWERKDIPMMPKGRYEI----MKKYMPKVGSLGLD 235

Query: 337 MMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWT 396
           MM RTCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFT+GK NG++SMRS IWT
Sbjct: 236 MMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTDGKPNGFVSMRSHIWT 295

Query: 397 DTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPV 456
           DTD  RTGMLPFVFDDSFGFE+YVD+ALDVPMYF+YRKKK+IDC+GM+FRDF+AGKL  +
Sbjct: 296 DTDKDRTGMLPFVFDDSFGFEQYVDFALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCI 355

Query: 457 PGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNA 516
           PGE PTL+DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVG+LYDEVSLQ  
Sbjct: 356 PGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQRV 415

Query: 517 LDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLN 576
           LD+TADWT EER+MLRN+V +TGL TPFRDGLLKHVA++V+ELAKDGL+RRGFKESGFLN
Sbjct: 416 LDMTADWTLEEREMLRNKVTVTGLKTPFRDGLLKHVAEEVLELAKDGLERRGFKESGFLN 475

Query: 577 EVTEVARTGVTLAEKMLGLYNGKWGQNVDPVFEELLY 579
            V EV RTGVT AE++L LY+GKW Q+VD VF+ELLY
Sbjct: 476 AVAEVVRTGVTPAERLLELYHGKWEQSVDHVFDELLY 508

[Close]
BLAST of Cp4.1LG05g03440 vs. Swiss-Prot
Match: GSH1_ARATH (Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana GN=GSH1 PE=1 SV=2)

HSP 1 Score: 689.1 bits (1777), Expect = 4.1e-197
Identity = 332/453 (73.29%), Postives = 374/453 (82.56%), Query Frame = 1 

Query: 157 RLIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTY 216
           +LIVA   P EEA VAT+PLT+EDL+AYLASGCK K+K+RIGTEHEKFGF++ +LRPM Y
Sbjct: 70  QLIVAASPPTEEAVVATEPLTREDLIAYLASGCKTKDKYRIGTEHEKFGFEVNTLRPMKY 129

Query: 217 EQISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEV 276
           +QI+ LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEV
Sbjct: 130 DQIAELLNGIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEV 189

Query: 277 NSHLYQGRYKIIE--------NYMPK----------VGSL-------------GLDMMLR 336
           NSHLYQ +    E         + PK           G               GLDMMLR
Sbjct: 190 NSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIMRNYMPKVGTLGLDMMLR 249

Query: 337 TCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDN 396
           TCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGK NG+LSMRS IWTDTD 
Sbjct: 250 TCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDK 309

Query: 397 SRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEF 456
            RTGMLPFVFDDSFGFE+YVDYALDVPMYF YRK K+IDC+GM+FR F+AGKL  +PGE 
Sbjct: 310 DRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFRQFLAGKLPCLPGEL 369

Query: 457 PTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDIT 516
           P+ +DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGLLYD+ SLQ  LD+T
Sbjct: 370 PSYNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDDDSLQAILDLT 429

Query: 517 ADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTE 576
           ADWTP ER+MLRN+VP+TGL TPFRDGLLKHVA+DV++LAKDGL+RRG+KE+GFLN V E
Sbjct: 430 ADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDE 489

Query: 577 VARTGVTLAEKMLGLYNGKWGQNVDPVFEELLY 579
           V RTGVT AEK+L +YNG+WGQ+VDPVFEELLY
Sbjct: 490 VVRTGVTPAEKLLEMYNGEWGQSVDPVFEELLY 522

[Close]
BLAST of Cp4.1LG05g03440 vs. Swiss-Prot
Match: GSH1_BRAJU (Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea GN=GSH1 PE=1 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 7.0e-197
Identity = 329/453 (72.63%), Postives = 373/453 (82.34%), Query Frame = 1 

Query: 157 RLIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTY 216
           +LIVA   P EEA VAT+PLT+EDL+AYLASGCK KEKWRIGTEHEKFGF++ +LRPM Y
Sbjct: 62  QLIVAASPPTEEAVVATEPLTREDLIAYLASGCKSKEKWRIGTEHEKFGFEVNTLRPMKY 121

Query: 217 EQISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEV 276
           +QI+ LLN +AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEV
Sbjct: 122 DQIAELLNSIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEV 181

Query: 277 NSHLYQGRYKIIE--------NYMPK-----VGSL------------------GLDMMLR 336
           NSHLYQ +    E         + PK     + ++                  GLDMMLR
Sbjct: 182 NSHLYQVKAVAEEMGIGFLGMGFQPKWRREDIPTMPKGRYDIMRNYMPKVGSLGLDMMLR 241

Query: 337 TCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDN 396
           TCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGK NG+LSMRS IWTDTD 
Sbjct: 242 TCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDK 301

Query: 397 SRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEF 456
            RTGMLPFVFDDSFGFE+YVDYALDVPMYF YR  K++DC+GM+FR F+AGKL  +PGE 
Sbjct: 302 DRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRNGKYVDCTGMTFRQFLAGKLPCLPGEL 361

Query: 457 PTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDIT 516
           PT +DWENHLTTI+PEVRLKRY+EMRG DGGPWRR+CALPAFWVGLLYDE  LQ+ LD+T
Sbjct: 362 PTYNDWENHLTTIFPEVRLKRYMEMRGADGGPWRRLCALPAFWVGLLYDEDVLQSVLDLT 421

Query: 517 ADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTE 576
           ADWTP ER+MLRN+VP+TGL TPFRDGLLKHVA+DV++LAKDGL+RRG+KE GFLN VTE
Sbjct: 422 ADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEVGFLNAVTE 481

Query: 577 VARTGVTLAEKMLGLYNGKWGQNVDPVFEELLY 579
           V RTGVT AE +L +YNG+WGQ+VDPVF+ELLY
Sbjct: 482 VVRTGVTPAENLLEMYNGEWGQSVDPVFQELLY 514

[Close]
BLAST of Cp4.1LG05g03440 vs. TrEMBL
Match: A0A0A0KK91_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G153150 PE=4 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 6.8e-215
Identity = 377/501 (75.25%), Postives = 403/501 (80.44%), Query Frame = 1 

Query: 109 SSGNLADGLRIDSARMRSKRGGRVIVATGAPAEEATVAGQREDEVEAWRLIVATGAPAEE 168
           SSGN+  GL IDSARM SKRGGRVIVA   P                           E+
Sbjct: 27  SSGNIMHGLHIDSARMASKRGGRVIVAASPPT--------------------------ED 86

Query: 169 ATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAE 228
           ATVA +PLTKEDLVAYLASGCKPKEKWRIGTEHEKFGF I +LRPMTY+QI+GLLNG+AE
Sbjct: 87  ATVAAEPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFNIETLRPMTYDQIAGLLNGIAE 146

Query: 229 RFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQGRYKII 288
           RF WEK+MEG +IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNSHLYQ +    
Sbjct: 147 RFDWEKIMEGDNIIGLQQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAE 206

Query: 289 E--------NYMPK----------VGSL-------------GLDMMLRTCTVQVNLDFDS 348
           E         + PK           G               GLDMM RTCTVQVNLDFDS
Sbjct: 207 EMGIGFLGIGFQPKWRREDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFDS 266

Query: 349 EADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSRTGMLPFVFDD 408
           EADMIRKFR GLALQPIATALFANSPFT+GK NGYLSMRSQIWTDTDN RTGMLPFVFDD
Sbjct: 267 EADMIRKFRAGLALQPIATALFANSPFTDGKPNGYLSMRSQIWTDTDNDRTGMLPFVFDD 326

Query: 409 SFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTT 468
           SFGFEKYVDYALDVPMYFIYRKKK+IDCSGMSFRDFMAGKL PVPGE PTL+DWENHLTT
Sbjct: 327 SFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLPPVPGELPTLTDWENHLTT 386

Query: 469 IYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITADWTPEERQMLR 528
           I+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGLLYDEVSLQN LDI ADWT  ERQMLR
Sbjct: 387 IFPEVRLKRYLEMRGSDGGPWRRLCALPAFWVGLLYDEVSLQNVLDIIADWTQGERQMLR 446

Query: 529 NEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKM 579
           N+VP++GL TPFRDGL+KHVAQD+V+LAKDGL+RRGFKESGFLNEVTEVARTGVT AEK+
Sbjct: 447 NKVPVSGLKTPFRDGLVKHVAQDIVKLAKDGLERRGFKESGFLNEVTEVARTGVTPAEKL 501

[Close]
BLAST of Cp4.1LG05g03440 vs. TrEMBL
Match: K9JCZ2_CUCSA (Gamma-glutamylcysteine synthetase OS=Cucumis sativus GN=GSH1 PE=2 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 9.8e-214
Identity = 388/553 (70.16%), Postives = 419/553 (75.77%), Query Frame = 1 

Query: 57  RIDSARMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWSSGNLADG 116
           RI +  +R  RGG +       AE   +      KE L+   +  C      SSGN+  G
Sbjct: 12  RIGAETLRC-RGGNMAFNFSTHAESLKM------KEILLGSSSLSC------SSGNIMHG 71

Query: 117 LRIDSARMRSKRGGRVIVATGAPAEEATVAGQREDEVEAWRLIVATGAPAEEATVATQPL 176
           L IDSARM SKRGGRVIVA   P                           E+ATVA +PL
Sbjct: 72  LHIDSARMASKRGGRVIVAASPPT--------------------------EDATVAAEPL 131

Query: 177 TKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAERFGWEKVM 236
           TKEDLVAYLASGCKPKEKWRIGTEHEKFGF I +LRPM YEQI+GLLNG+AERF WEK+M
Sbjct: 132 TKEDLVAYLASGCKPKEKWRIGTEHEKFGFNIETLRPMKYEQIAGLLNGIAERFDWEKIM 191

Query: 237 EGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQGRYKIIE------- 296
           EG +IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNSHLYQ +    E       
Sbjct: 192 EGDNIIGLQQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLG 251

Query: 297 -NYMPK----------VGSL-------------GLDMMLRTCTVQVNLDFDSEADMIRKF 356
             + PK           G               GLDMM RTCTVQVNLDFDSEADMIRKF
Sbjct: 252 IGFQPKWRREDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFDSEADMIRKF 311

Query: 357 RVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYV 416
           R G ALQPIATALFANSPFT+GK NGYLSMRSQIWTDTDN RTGMLPFVFDDSFGFEKYV
Sbjct: 312 RAGFALQPIATALFANSPFTDGKPNGYLSMRSQIWTDTDNDRTGMLPFVFDDSFGFEKYV 371

Query: 417 DYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLK 476
           DYALDVPMYFIYRKKK+IDCSGMSFRDFMAGKL PVPGE PTL+DWENHLTTI+PEVRLK
Sbjct: 372 DYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLPPVPGELPTLTDWENHLTTIFPEVRLK 431

Query: 477 RYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITADWTPEERQMLRNEVPITGL 536
           RYLEMRG DGGPWRR+CALPAFWVGLLYDEVSLQN LDI ADWT  ERQMLRN+VP++GL
Sbjct: 432 RYLEMRGSDGGPWRRLCALPAFWVGLLYDEVSLQNVLDIIADWTQGERQMLRNKVPVSGL 491

Query: 537 NTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKMLGLYNGKW 579
            TPFRDGL+KHVAQD+V+LAKDGL+RRGFKESGFLNEVTEVARTGVT AEK+L LYNGKW
Sbjct: 492 KTPFRDGLVKHVAQDIVKLAKDGLERRGFKESGFLNEVTEVARTGVTPAEKLLELYNGKW 525

[Close]
BLAST of Cp4.1LG05g03440 vs. TrEMBL
Match: W9QH55_9ROSA (Glutamate--cysteine ligase OS=Morus notabilis GN=L484_024282 PE=4 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 5.8e-206
Identity = 370/556 (66.55%), Postives = 412/556 (74.10%), Query Frame = 1 

Query: 58  IDSARMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWSSGNLADGL 117
           + S  +RSK G   I   G+  E + V      K++ V   +  C    K ++      L
Sbjct: 13  VQSEVIRSKSGHNAIFNIGSNLEASRV------KDNFVGASSLSCTFAAKKTNI-----L 72

Query: 118 RIDSARMRSKRGGRVIVATGAPAEEATVAGQREDEVEAWRLIVATGAPAEEATVATQPLT 177
            +D AR+  KRG  VIVA   P E+A                           VA +PLT
Sbjct: 73  HLDCARLGRKRGHHVIVAASPPTEDA---------------------------VAAEPLT 132

Query: 178 KEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAERFGWEKVME 237
           KEDL+AYLASGCKPKEKWRIGTEHEKFGF+ G+LRPM YEQIS LLNG+AERF W+KVME
Sbjct: 133 KEDLIAYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMRYEQISELLNGIAERFDWDKVME 192

Query: 238 GSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQ--------------- 297
           G  IIGL QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNSHLYQ               
Sbjct: 193 GDYIIGLIQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGI 252

Query: 298 ---------------------GRYKIIENYMPKVGSLGLDMMLRTCTVQVNLDFDSEADM 357
                                GRY+I+ NYMPKVGSLGLDMM RTCTVQVNLDF+SEADM
Sbjct: 253 GFQPKWGLKDIPVMPKRYLAQGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFNSEADM 312

Query: 358 IRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSRTGMLPFVFDDSFGF 417
           IRKFR GLALQPIATALFANSPFTEGK NG+LSMRSQIWTDTD  RTGMLPFVFDDSFGF
Sbjct: 313 IRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSQIWTDTDKDRTGMLPFVFDDSFGF 372

Query: 418 EKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTTIYPE 477
           E+YVDYALDVPMYF+YRKKK+IDC+GM+FRDF+AGKL  +PGE PTL+DWENHLTTI+PE
Sbjct: 373 EQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPE 432

Query: 478 VRLKRYLEMRGGDGGPWRRICALPAFW---VGLLYDEVSLQNALDITADWTPEERQMLRN 537
           VRLKRYLEMRG DGGPWRR+CALPAFW   VGLLYDEVSLQN LD+TADWTPEERQMLRN
Sbjct: 433 VRLKRYLEMRGADGGPWRRLCALPAFWNDQVGLLYDEVSLQNVLDMTADWTPEERQMLRN 492

Query: 538 EVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKML 575
           +VP TGL TPFRDG L+HVA+DV+ LAKDGL+RRGFKESGFLN V EV RTGVT AEK+L
Sbjct: 493 KVPKTGLKTPFRDGYLRHVAEDVLHLAKDGLERRGFKESGFLNAVAEVVRTGVTPAEKLL 530

[Close]
BLAST of Cp4.1LG05g03440 vs. TrEMBL
Match: B9RCB6_RICCO (Glutamate--cysteine ligase, chloroplast, putative OS=Ricinus communis GN=RCOM_1686670 PE=4 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 2.9e-205
Identity = 346/453 (76.38%), Postives = 382/453 (84.33%), Query Frame = 1 

Query: 157 RLIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTY 216
           ++IVA   P E+A +AT+PLTKEDLV YLASGCKPKEKWRIGTEHEKFGF++G+LRPM Y
Sbjct: 74  QMIVAASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFELGTLRPMKY 133

Query: 217 EQISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEV 276
           EQI+ LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEV
Sbjct: 134 EQIAELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEV 193

Query: 277 NSHLYQGRYKIIE--------NYMPKVGSL-----------------------GLDMMLR 336
           NSHLYQ +    E         + PK G                         GLDMM R
Sbjct: 194 NSHLYQVKAVTEEMGIGFLGIGFQPKWGVKDIPIMPKGRYEIMRNYMPKVGTLGLDMMFR 253

Query: 337 TCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDN 396
           TCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGK NGYLSMRSQIWTDTD 
Sbjct: 254 TCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQIWTDTDK 313

Query: 397 SRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEF 456
            RTGMLPFVFDDSFGFE+YVDYALDVPMYF+YRKKK+IDC+GM+FRDF+AGKL  +PGE 
Sbjct: 314 DRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEL 373

Query: 457 PTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDIT 516
           PTL+DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGLLYDE+SLQN LD+T
Sbjct: 374 PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEISLQNVLDMT 433

Query: 517 ADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTE 576
           ADWTPEER+MLRN+VP TGL TPFRDGLLKHVA+DV++LAKDGL+RRG+KE GFLNEVTE
Sbjct: 434 ADWTPEEREMLRNKVPKTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEIGFLNEVTE 493

Query: 577 VARTGVTLAEKMLGLYNGKWGQNVDPVFEELLY 579
           V RTGVT AE++L LYNGKWGQ+VDPVFEELLY
Sbjct: 494 VVRTGVTPAERLLELYNGKWGQSVDPVFEELLY 526

[Close]
BLAST of Cp4.1LG05g03440 vs. TrEMBL
Match: M5WP73_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004151mg PE=4 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 4.9e-205
Identity = 346/452 (76.55%), Postives = 382/452 (84.51%), Query Frame = 1 

Query: 158 LIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYE 217
           +IVA   P E+A + T+PLTKEDLV YLASGCKPKE WRIGTEHEKFGF++ +LRPM YE
Sbjct: 75  VIVAASPPTEDAVIVTEPLTKEDLVGYLASGCKPKENWRIGTEHEKFGFELKTLRPMKYE 134

Query: 218 QISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVN 277
           QI+ LLNG++ERF WEKVMEG  IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVN
Sbjct: 135 QIAELLNGISERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVN 194

Query: 278 SHLYQGRYKIIE--------NYMPK--VGSL---------------------GLDMMLRT 337
           SHLYQ +    E         + PK  +  +                     GLDMM RT
Sbjct: 195 SHLYQVKAVTEEMGIGFLGIGFQPKWRIKDIPIMPKGRYEIMRNYMPKVGTLGLDMMFRT 254

Query: 338 CTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNS 397
           CTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGK NG+LSMRSQIWTDTDN+
Sbjct: 255 CTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSQIWTDTDNN 314

Query: 398 RTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFP 457
           RTGMLPFVFDDSFGFE+YVDYALDVPMYF+YRKKK+IDC+GM+FRDF+AGKLS +PGE P
Sbjct: 315 RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLSCIPGELP 374

Query: 458 TLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITA 517
           TL+DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVG+LYDEV+LQN LDITA
Sbjct: 375 TLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVALQNVLDITA 434

Query: 518 DWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEV 577
           DWTPEERQMLRN+VPITGL TPFRDGLLKHVAQDVV+LAKDGL+RRGFKESGFLNEV EV
Sbjct: 435 DWTPEERQMLRNKVPITGLKTPFRDGLLKHVAQDVVKLAKDGLERRGFKESGFLNEVAEV 494

Query: 578 ARTGVTLAEKMLGLYNGKWGQNVDPVFEELLY 579
            RTGVT AE++L LYNGKWGQ +DPVFEELLY
Sbjct: 495 VRTGVTPAERLLELYNGKWGQQIDPVFEELLY 526

[Close]
BLAST of Cp4.1LG05g03440 vs. TAIR10
Match: AT4G23100.1 (AT4G23100.1 glutamate-cysteine ligase)

HSP 1 Score: 689.1 bits (1777), Expect = 2.3e-198
Identity = 332/453 (73.29%), Postives = 374/453 (82.56%), Query Frame = 1 

Query: 157 RLIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTY 216
           +LIVA   P EEA VAT+PLT+EDL+AYLASGCK K+K+RIGTEHEKFGF++ +LRPM Y
Sbjct: 70  QLIVAASPPTEEAVVATEPLTREDLIAYLASGCKTKDKYRIGTEHEKFGFEVNTLRPMKY 129

Query: 217 EQISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEV 276
           +QI+ LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEV
Sbjct: 130 DQIAELLNGIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEV 189

Query: 277 NSHLYQGRYKIIE--------NYMPK----------VGSL-------------GLDMMLR 336
           NSHLYQ +    E         + PK           G               GLDMMLR
Sbjct: 190 NSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIMRNYMPKVGTLGLDMMLR 249

Query: 337 TCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDN 396
           TCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGK NG+LSMRS IWTDTD 
Sbjct: 250 TCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDK 309

Query: 397 SRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEF 456
            RTGMLPFVFDDSFGFE+YVDYALDVPMYF YRK K+IDC+GM+FR F+AGKL  +PGE 
Sbjct: 310 DRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFRQFLAGKLPCLPGEL 369

Query: 457 PTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDIT 516
           P+ +DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGLLYD+ SLQ  LD+T
Sbjct: 370 PSYNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDDDSLQAILDLT 429

Query: 517 ADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTE 576
           ADWTP ER+MLRN+VP+TGL TPFRDGLLKHVA+DV++LAKDGL+RRG+KE+GFLN V E
Sbjct: 430 ADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDE 489

Query: 577 VARTGVTLAEKMLGLYNGKWGQNVDPVFEELLY 579
           V RTGVT AEK+L +YNG+WGQ+VDPVFEELLY
Sbjct: 490 VVRTGVTPAEKLLEMYNGEWGQSVDPVFEELLY 522

[Close]
BLAST of Cp4.1LG05g03440 vs. NCBI nr
Match: gi|659073819|ref|XP_008437270.1| (PREDICTED: glutamate--cysteine ligase, chloroplastic [Cucumis melo])

HSP 1 Score: 756.9 bits (1953), Expect = 2.6e-215
Identity = 376/501 (75.05%), Postives = 404/501 (80.64%), Query Frame = 1 

Query: 109 SSGNLADGLRIDSARMRSKRGGRVIVATGAPAEEATVAGQREDEVEAWRLIVATGAPAEE 168
           SS N+  GL IDSAR  S+RG RVIVA   P                           E+
Sbjct: 51  SSRNIMHGLHIDSARTASRRGDRVIVAASPPT--------------------------ED 110

Query: 169 ATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAE 228
           ATVAT+PLTKEDLVAYLASGCKPKEKWRIGTEHEKFGF I +LRPMTYEQI+GLLNG+AE
Sbjct: 111 ATVATEPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFNIETLRPMTYEQIAGLLNGIAE 170

Query: 229 RFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQGRYKII 288
           RF WEK+MEG +IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNSHLYQ +    
Sbjct: 171 RFDWEKIMEGDNIIGLQQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAE 230

Query: 289 E--------NYMPKVGSL-----------------------GLDMMLRTCTVQVNLDFDS 348
           E         + PK                           GLDMM RTCTVQVNLDFDS
Sbjct: 231 EMGIGFLGIGFQPKWSREDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFDS 290

Query: 349 EADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSRTGMLPFVFDD 408
           EADMI+KFR GLALQPIATALFANSPFT+GK NGYLSMRSQIWTDTDN RTGMLPFVFDD
Sbjct: 291 EADMIKKFRAGLALQPIATALFANSPFTDGKPNGYLSMRSQIWTDTDNDRTGMLPFVFDD 350

Query: 409 SFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTT 468
           SFGFEKYVDYALDVPMYFIYRKKK+IDCSGMSFRDFMAGKL PVPGE PTL+DWENHLTT
Sbjct: 351 SFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLPPVPGELPTLTDWENHLTT 410

Query: 469 IYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITADWTPEERQMLR 528
           I+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGLLYDEVSLQN LDITADWT  ERQMLR
Sbjct: 411 IFPEVRLKRYLEMRGSDGGPWRRLCALPAFWVGLLYDEVSLQNVLDITADWTQGERQMLR 470

Query: 529 NEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKM 579
           N+VP++GLNTPFRDGL+KHVAQD+V+LAKDGL+RRGFKESGFLNEVTEVARTGVT AEK+
Sbjct: 471 NKVPVSGLNTPFRDGLVKHVAQDIVKLAKDGLERRGFKESGFLNEVTEVARTGVTPAEKL 525

[Close]
BLAST of Cp4.1LG05g03440 vs. NCBI nr
Match: gi|700194923|gb|KGN50100.1| (hypothetical protein Csa_5G153150 [Cucumis sativus])

HSP 1 Score: 755.0 bits (1948), Expect = 9.8e-215
Identity = 377/501 (75.25%), Postives = 403/501 (80.44%), Query Frame = 1 

Query: 109 SSGNLADGLRIDSARMRSKRGGRVIVATGAPAEEATVAGQREDEVEAWRLIVATGAPAEE 168
           SSGN+  GL IDSARM SKRGGRVIVA   P                           E+
Sbjct: 27  SSGNIMHGLHIDSARMASKRGGRVIVAASPPT--------------------------ED 86

Query: 169 ATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAE 228
           ATVA +PLTKEDLVAYLASGCKPKEKWRIGTEHEKFGF I +LRPMTY+QI+GLLNG+AE
Sbjct: 87  ATVAAEPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFNIETLRPMTYDQIAGLLNGIAE 146

Query: 229 RFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQGRYKII 288
           RF WEK+MEG +IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNSHLYQ +    
Sbjct: 147 RFDWEKIMEGDNIIGLQQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAE 206

Query: 289 E--------NYMPK----------VGSL-------------GLDMMLRTCTVQVNLDFDS 348
           E         + PK           G               GLDMM RTCTVQVNLDFDS
Sbjct: 207 EMGIGFLGIGFQPKWRREDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFDS 266

Query: 349 EADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSRTGMLPFVFDD 408
           EADMIRKFR GLALQPIATALFANSPFT+GK NGYLSMRSQIWTDTDN RTGMLPFVFDD
Sbjct: 267 EADMIRKFRAGLALQPIATALFANSPFTDGKPNGYLSMRSQIWTDTDNDRTGMLPFVFDD 326

Query: 409 SFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTT 468
           SFGFEKYVDYALDVPMYFIYRKKK+IDCSGMSFRDFMAGKL PVPGE PTL+DWENHLTT
Sbjct: 327 SFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLPPVPGELPTLTDWENHLTT 386

Query: 469 IYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITADWTPEERQMLR 528
           I+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGLLYDEVSLQN LDI ADWT  ERQMLR
Sbjct: 387 IFPEVRLKRYLEMRGSDGGPWRRLCALPAFWVGLLYDEVSLQNVLDIIADWTQGERQMLR 446

Query: 529 NEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKM 579
           N+VP++GL TPFRDGL+KHVAQD+V+LAKDGL+RRGFKESGFLNEVTEVARTGVT AEK+
Sbjct: 447 NKVPVSGLKTPFRDGLVKHVAQDIVKLAKDGLERRGFKESGFLNEVTEVARTGVTPAEKL 501

[Close]
BLAST of Cp4.1LG05g03440 vs. NCBI nr
Match: gi|778699622|ref|XP_011654747.1| (PREDICTED: glutamate--cysteine ligase, chloroplastic [Cucumis sativus])

HSP 1 Score: 753.8 bits (1945), Expect = 2.2e-214
Identity = 389/553 (70.34%), Postives = 421/553 (76.13%), Query Frame = 1 

Query: 57  RIDSARMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWSSGNLADG 116
           RI +  +R  RGG +       AE   +      KE L+   +  C      SSGN+  G
Sbjct: 12  RIGAETLRC-RGGNMAFNFSTHAESLKM------KEILLGSSSLSC------SSGNIMHG 71

Query: 117 LRIDSARMRSKRGGRVIVATGAPAEEATVAGQREDEVEAWRLIVATGAPAEEATVATQPL 176
           L IDSARM SKRGGRVIVA   P                           E+ATVA +PL
Sbjct: 72  LHIDSARMASKRGGRVIVAASPPT--------------------------EDATVAAEPL 131

Query: 177 TKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAERFGWEKVM 236
           TKEDLVAYLASGCKPKEKWRIGTEHEKFGF I +LRPMTY+QI+GLLNG+AERF WEK+M
Sbjct: 132 TKEDLVAYLASGCKPKEKWRIGTEHEKFGFNIETLRPMTYDQIAGLLNGIAERFDWEKIM 191

Query: 237 EGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQGRYKIIE------- 296
           EG +IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNSHLYQ +    E       
Sbjct: 192 EGDNIIGLQQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLG 251

Query: 297 -NYMPK----------VGSL-------------GLDMMLRTCTVQVNLDFDSEADMIRKF 356
             + PK           G               GLDMM RTCTVQVNLDFDSEADMIRKF
Sbjct: 252 IGFQPKWRREDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFDSEADMIRKF 311

Query: 357 RVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYV 416
           R GLALQPIATALFANSPFT+GK NGYLSMRSQIWTDTDN RTGMLPFVFDDSFGFEKYV
Sbjct: 312 RAGLALQPIATALFANSPFTDGKPNGYLSMRSQIWTDTDNDRTGMLPFVFDDSFGFEKYV 371

Query: 417 DYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLK 476
           DYALDVPMYFIYRKKK+IDCSGMSFRDFMAGKL PVPGE PTL+DWENHLTTI+PEVRLK
Sbjct: 372 DYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLPPVPGELPTLTDWENHLTTIFPEVRLK 431

Query: 477 RYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITADWTPEERQMLRNEVPITGL 536
           RYLEMRG DGGPWRR+CALPAFWVGLLYDEVSLQN LDI ADWT  ERQMLRN+VP++GL
Sbjct: 432 RYLEMRGSDGGPWRRLCALPAFWVGLLYDEVSLQNVLDIIADWTQGERQMLRNKVPVSGL 491

Query: 537 NTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKMLGLYNGKW 579
            TPFRDGL+KHVAQD+V+LAKDGL+RRGFKESGFLNEVTEVARTGVT AEK+L LYNGKW
Sbjct: 492 KTPFRDGLVKHVAQDIVKLAKDGLERRGFKESGFLNEVTEVARTGVTPAEKLLELYNGKW 525

[Close]
BLAST of Cp4.1LG05g03440 vs. NCBI nr
Match: gi|793418194|ref|NP_001292626.1| (glutamate--cysteine ligase, chloroplastic [Cucumis sativus])

HSP 1 Score: 751.1 bits (1938), Expect = 1.4e-213
Identity = 388/553 (70.16%), Postives = 419/553 (75.77%), Query Frame = 1 

Query: 57  RIDSARMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWSSGNLADG 116
           RI +  +R  RGG +       AE   +      KE L+   +  C      SSGN+  G
Sbjct: 12  RIGAETLRC-RGGNMAFNFSTHAESLKM------KEILLGSSSLSC------SSGNIMHG 71

Query: 117 LRIDSARMRSKRGGRVIVATGAPAEEATVAGQREDEVEAWRLIVATGAPAEEATVATQPL 176
           L IDSARM SKRGGRVIVA   P                           E+ATVA +PL
Sbjct: 72  LHIDSARMASKRGGRVIVAASPPT--------------------------EDATVAAEPL 131

Query: 177 TKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAERFGWEKVM 236
           TKEDLVAYLASGCKPKEKWRIGTEHEKFGF I +LRPM YEQI+GLLNG+AERF WEK+M
Sbjct: 132 TKEDLVAYLASGCKPKEKWRIGTEHEKFGFNIETLRPMKYEQIAGLLNGIAERFDWEKIM 191

Query: 237 EGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQGRYKIIE------- 296
           EG +IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNSHLYQ +    E       
Sbjct: 192 EGDNIIGLQQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLG 251

Query: 297 -NYMPK----------VGSL-------------GLDMMLRTCTVQVNLDFDSEADMIRKF 356
             + PK           G               GLDMM RTCTVQVNLDFDSEADMIRKF
Sbjct: 252 IGFQPKWRREDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFDSEADMIRKF 311

Query: 357 RVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYV 416
           R G ALQPIATALFANSPFT+GK NGYLSMRSQIWTDTDN RTGMLPFVFDDSFGFEKYV
Sbjct: 312 RAGFALQPIATALFANSPFTDGKPNGYLSMRSQIWTDTDNDRTGMLPFVFDDSFGFEKYV 371

Query: 417 DYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLK 476
           DYALDVPMYFIYRKKK+IDCSGMSFRDFMAGKL PVPGE PTL+DWENHLTTI+PEVRLK
Sbjct: 372 DYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLPPVPGELPTLTDWENHLTTIFPEVRLK 431

Query: 477 RYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITADWTPEERQMLRNEVPITGL 536
           RYLEMRG DGGPWRR+CALPAFWVGLLYDEVSLQN LDI ADWT  ERQMLRN+VP++GL
Sbjct: 432 RYLEMRGSDGGPWRRLCALPAFWVGLLYDEVSLQNVLDIIADWTQGERQMLRNKVPVSGL 491

Query: 537 NTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKMLGLYNGKW 579
            TPFRDGL+KHVAQD+V+LAKDGL+RRGFKESGFLNEVTEVARTGVT AEK+L LYNGKW
Sbjct: 492 KTPFRDGLVKHVAQDIVKLAKDGLERRGFKESGFLNEVTEVARTGVTPAEKLLELYNGKW 525

[Close]
BLAST of Cp4.1LG05g03440 vs. NCBI nr
Match: gi|764580654|ref|XP_011463882.1| (PREDICTED: glutamate--cysteine ligase, chloroplastic [Fragaria vesca subsp. vesca])

HSP 1 Score: 746.1 bits (1925), Expect = 4.5e-212
Identity = 353/452 (78.10%), Postives = 389/452 (86.06%), Query Frame = 1 

Query: 158 LIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYE 217
           ++VA   P E+A +AT+PLTKEDLVAYLASGCKPKE WRIGTEHEKFGF++ +LRPM YE
Sbjct: 65  VVVAASPPTEDAVIATEPLTKEDLVAYLASGCKPKEAWRIGTEHEKFGFELKTLRPMKYE 124

Query: 218 QISGLLNGLAERFGWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVN 277
           QIS LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVN
Sbjct: 125 QISELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVN 184

Query: 278 SHLYQ-------------------------------GRYKIIENYMPKVGSLGLDMMLRT 337
           SHLYQ                               GRY+I++NYMPKVG+LGLDMM RT
Sbjct: 185 SHLYQVKAVTEEMGIGFLGIGFQPKWGLKDIPMMPKGRYQIMKNYMPKVGTLGLDMMFRT 244

Query: 338 CTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKSNGYLSMRSQIWTDTDNS 397
           CTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGK NG+LSMRSQIWTDTDN+
Sbjct: 245 CTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSQIWTDTDNN 304

Query: 398 RTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKFIDCSGMSFRDFMAGKLSPVPGEFP 457
           RTGMLPFVFDDSFGFE+YVDYALDVPMYF+YR KK+IDC+GM+FRDF+ GKL  +PG+ P
Sbjct: 305 RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRNKKYIDCTGMTFRDFLKGKLRCIPGDLP 364

Query: 458 TLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITA 517
           TL+DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVG+LYDEVSLQN LDITA
Sbjct: 365 TLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQNVLDITA 424

Query: 518 DWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVELAKDGLKRRGFKESGFLNEVTEV 577
           DWTPEERQMLRN+VPITGL TPFRDGLLKHVAQDVV+LAKDGL+RRG+KESGFLNEV EV
Sbjct: 425 DWTPEERQMLRNKVPITGLKTPFRDGLLKHVAQDVVKLAKDGLERRGYKESGFLNEVAEV 484

Query: 578 ARTGVTLAEKMLGLYNGKWGQNVDPVFEELLY 579
            RTGVT AE++L LYNGKW Q +DPVFEELLY
Sbjct: 485 VRTGVTPAERLLELYNGKWEQQIDPVFEELLY 516

The following BLAST results are available for this feature:
BLAST of Cp4.1LG05g03440 vs. Swiss-Prot
Match NameE-valueIdentityDescription
GSH1_TOBAC3.1e-20576.05Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum GN=GSH1 PE=2 SV=2[more]
GSH1_SOLLC1.8e-20074.28Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum GN=GSH1 PE=2 S... [more]
GSH1_MEDTR1.7e-19873.30Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula GN=GSH1 PE=2 SV... [more]
GSH1_ARATH4.1e-19773.29Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana GN=GSH1 PE=1 S... [more]
GSH1_BRAJU7.0e-19772.63Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea GN=GSH1 PE=1 SV=1[more]
BLAST of Cp4.1LG05g03440 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KK91_CUCSA6.8e-21575.25Uncharacterized protein OS=Cucumis sativus GN=Csa_5G153150 PE=4 SV=1[more]
K9JCZ2_CUCSA9.8e-21470.16Gamma-glutamylcysteine synthetase OS=Cucumis sativus GN=GSH1 PE=2 SV=1[more]
W9QH55_9ROSA5.8e-20666.55Glutamate--cysteine ligase OS=Morus notabilis GN=L484_024282 PE=4 SV=1[more]
B9RCB6_RICCO2.9e-20576.38Glutamate--cysteine ligase, chloroplast, putative OS=Ricinus communis GN=RCOM_16... [more]
M5WP73_PRUPE4.9e-20576.55Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004151mg PE=4 SV=1[more]
BLAST of Cp4.1LG05g03440 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G23100.12.3e-19873.29 glutamate-cysteine ligase[more]
BLAST of Cp4.1LG05g03440 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659073819|ref|XP_008437270.1|2.6e-21575.05PREDICTED: glutamate--cysteine ligase, chloroplastic [Cucumis melo][more]
gi|700194923|gb|KGN50100.1|9.8e-21575.25hypothetical protein Csa_5G153150 [Cucumis sativus][more]
gi|778699622|ref|XP_011654747.1|2.2e-21470.34PREDICTED: glutamate--cysteine ligase, chloroplastic [Cucumis sativus][more]
gi|793418194|ref|NP_001292626.1|1.4e-21370.16glutamate--cysteine ligase, chloroplastic [Cucumis sativus][more]
gi|764580654|ref|XP_011463882.1|4.5e-21278.10PREDICTED: glutamate--cysteine ligase, chloroplastic [Fragaria vesca subsp. vesc... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006750glutathione biosynthetic process
GO:0042398cellular modified amino acid biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0004357glutamate-cysteine ligase activity
Vocabulary: INTERPRO
TermDefinition
IPR011556Glut_cys_lig_pln_type
IPR006336GCS2
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
biological_process GO:0006006 glucose metabolic process
biological_process GO:0016051 carbohydrate biosynthetic process
biological_process GO:0019752 carboxylic acid metabolic process
biological_process GO:0006725 cellular aromatic compound metabolic process
biological_process GO:0042398 cellular modified amino acid biosynthetic process
biological_process GO:0044271 cellular nitrogen compound biosynthetic process
biological_process GO:0042742 defense response to bacterium
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0009408 response to heat
biological_process GO:1901657 glycosyl compound metabolic process
biological_process GO:0046483 heterocycle metabolic process
biological_process GO:1901360 organic cyclic compound metabolic process
biological_process GO:0009628 response to abiotic stimulus
biological_process GO:0044550 secondary metabolite biosynthetic process
biological_process GO:0044724 single-organism carbohydrate catabolic process
biological_process GO:0044272 sulfur compound biosynthetic process
biological_process GO:0006750 glutathione biosynthetic process
biological_process GO:0009651 response to salt stress
biological_process GO:0046686 response to cadmium ion
biological_process GO:0002213 defense response to insect
biological_process GO:0044763 single-organism cellular process
biological_process GO:0030244 cellulose biosynthetic process
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0052544 defense response by callose deposition in cell wall
biological_process GO:0009700 indole phytoalexin biosynthetic process
biological_process GO:0050832 defense response to fungus
biological_process GO:0009816 defense response to bacterium, incompatible interaction
biological_process GO:0009908 flower development
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006007 glucose catabolic process
biological_process GO:0019761 glucosinolate biosynthetic process
biological_process GO:0006096 glycolytic process
cellular_component GO:0009536 plastid
cellular_component GO:0017109 glutamate-cysteine ligase complex
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0009507 chloroplast
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004357 glutamate-cysteine ligase activity
molecular_function GO:0000166 nucleotide binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g03440.1Cp4.1LG05g03440.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006336Glutamate--cysteine ligase, GCS2PFAMPF04107GCS2coord: 282..488
score: 2.6E-66coord: 226..280
score: 3.
IPR011556Glutamate--cysteine ligase, plantTIGRFAMsTIGR01436TIGR01436coord: 282..578
score: 1.1E
NoneNo IPR availablePANTHERPTHR34378FAMILY NOT NAMEDcoord: 115..578
score:
NoneNo IPR availablePANTHERPTHR34378:SF1GLUTAMATE--CYSTEINE LIGASE, CHLOROPLASTICcoord: 115..578
score:
NoneNo IPR availableunknownSSF55931Glutamine synthetase/guanido kinasecoord: 434..576
score: 2.75E-56coord: 187..395
score: 2.75

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG05g03440Cucsa.303150Cucumber (Gy14) v1cgycpeB0792
Cp4.1LG05g03440CmaCh15G012250Cucurbita maxima (Rimu)cmacpeB329
Cp4.1LG05g03440CmaCh02G014540Cucurbita maxima (Rimu)cmacpeB647
Cp4.1LG05g03440CmoCh02G014890Cucurbita moschata (Rifu)cmocpeB596
Cp4.1LG05g03440CmoCh15G012900Cucurbita moschata (Rifu)cmocpeB292
Cp4.1LG05g03440Cla011449Watermelon (97103) v1cpewmB717
Cp4.1LG05g03440Csa5G153150Cucumber (Chinese Long) v2cpecuB736
Cp4.1LG05g03440MELO3C005674Melon (DHL92) v3.5.1cpemeB647
Cp4.1LG05g03440ClCG01G002740Watermelon (Charleston Gray)cpewcgB639
Cp4.1LG05g03440CSPI05G05430Wild cucumber (PI 183967)cpecpiB738
Cp4.1LG05g03440Lsi09G002610Bottle gourd (USVL1VR-Ls)cpelsiB582
Cp4.1LG05g03440MELO3C005674.2Melon (DHL92) v3.6.1cpemedB774
Cp4.1LG05g03440CsaV3_5G002830Cucumber (Chinese Long) v3cpecucB0915
Cp4.1LG05g03440Bhi12G000244Wax gourdcpewgoB0910
Cp4.1LG05g03440CsGy5G002670Cucumber (Gy14) v2cgybcpeB691
Cp4.1LG05g03440Carg16637Silver-seed gourdcarcpeB1298
Cp4.1LG05g03440Carg02528Silver-seed gourdcarcpeB1282
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG05g03440Cp4.1LG13g01530Cucurbita pepo (Zucchini)cpecpeB226
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG05g03440Melon (DHL92) v3.6.1cpemedB831
Cp4.1LG05g03440Cucumber (Chinese Long) v3cpecucB0896
Cp4.1LG05g03440Wax gourdcpewgoB0929
Cp4.1LG05g03440Watermelon (Charleston Gray)cpewcgB680
Cp4.1LG05g03440Cucumber (Gy14) v2cgybcpeB427