Cp4.1LG05g03350 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG05g03350 vs. Swiss-Prot
Match: MURE_BACLD (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) GN=murE PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.7e-90
Identity = 199/515 (38.64%), Postives = 297/515 (57.67%), Query Frame = 1

Query: 246 MTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLYLTEADK 305
           M L +LL   K VP S  G  + +IT I+ DSR V  G LFVC  G   DGH Y  +A +
Sbjct: 1   MKLTKLLTYLKNVP-SYAGQEDPDITSIEMDSREVKTGSLFVCIKGYTVDGHDYARQAAE 60

Query: 306 RGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVTGTDGKT 365
           +GA A+VA +E+D +  +     +++  T   L  L+ +FY  P+K + +IG+TGT+GKT
Sbjct: 61  KGAAAIVAEREVDADVPV-----IIIRHTKRALAVLSDAFYGQPTKQLRLIGITGTNGKT 120

Query: 366 STSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLHNGTEAV 425
           STS++++ ++   G RTGL+GT+   +H D   EA +TTP+++ +Q    KM+  G +  
Sbjct: 121 STSHMVEEIFRKAGSRTGLIGTMYTKIH-DETFEAKNTTPESVTLQKTFRKMVDQGVDTA 180

Query: 426 VMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMVDPDRHR 485
           VMEVS+  L  GR    D+DIA FTNLT + +LD+ E+ EEY+ AK+ LF ++     H 
Sbjct: 181 VMEVSSHALHMGRVHGCDYDIAAFTNLT-QDHLDYHETMEEYKHAKSLLFSQLGGSFNHE 240

Query: 486 K----VVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQG 545
                V+N DDP + +F    +  +  +T+ ++N +A V     +++   T   + TP+G
Sbjct: 241 TPKWAVLNADDPASAYFAQVTSAHL--LTYGIQN-DADVMAENIKMAPKGTTFDLVTPKG 300

Query: 546 ILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGVI 605
             +++  L+G  N+YN+L A ++ IA   P   I  GIE +  V GRFEL+D  Q F VI
Sbjct: 301 SAQVTIPLVGLFNVYNVLTAAAIAIAADIPFATITEGIEGLKGVRGRFELVDAGQDFPVI 360

Query: 606 VDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSDN 665
           VD A TPD L  +L++ R L   ++  V GC G+ D+ KRP M KIA D +D  + T+DN
Sbjct: 361 VDYAHTPDSLENVLNTCRGLTEGKLFVVVGCGGDRDKTKRPKMAKIAVDLADEPVFTADN 420

Query: 666 PRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMGE 725
           PR+E+PL IL+DM  G+           +  YY  + N         R  A+   +A  +
Sbjct: 421 PRSENPLAILNDMEEGV-----------KGAYYHSIVN---------REQAIFFAIANAK 480

Query: 726 EGDVVVVAGKGHETYQIEGDKKDFFDDREECREAL 757
           +GDVV++AGKGHETYQ  G +   FDD E  + A+
Sbjct: 481 KGDVVLIAGKGHETYQQIGGQTFDFDDAEVAKRAI 484

BLAST of Cp4.1LG05g03350 vs. Swiss-Prot
Match: MURE_BACVZ (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase OS=Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) GN=murE PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-88
Identity = 189/492 (38.41%), Postives = 288/492 (58.54%), Query Frame = 1

Query: 269 EITGIQHDSRMVNAGYLFVCCVGRETDGHLYLTEADKRGAVAVVASKEIDIEETLGCKAL 328
           +IT I+ DSR V  G LFVC  G   DGH +  +A + GA A+VA KE+D++  +     
Sbjct: 23  DITSIEMDSREVRKGSLFVCIKGYTVDGHDFAQKAAENGAAAIVAEKELDVDVPV----- 82

Query: 329 VMVEDTNSVLPALAASFYMNPSKNMAVIGVTGTDGKTSTSYLIKGMYEAMGLRTGLLGTV 388
           ++V  +   L  L+ +FY  P+K + +IG+TGT+GKTST++++  +++  G +TGL+GT+
Sbjct: 83  IIVRRSQRALSVLSDAFYGQPTKQLQLIGITGTNGKTSTTHMVDEIFKKAGRQTGLIGTM 142

Query: 389 AYYLHGDNKLEAPSTTPDAILVQNLMAKMLHNGTEAVVMEVSTAELARGRCDEVDFDIAV 448
            Y   GD      +TTP+++ +Q    KM     +  +MEVS+  L+ GR    D+DIAV
Sbjct: 143 -YIKIGDETFPVKNTTPESVTLQKTFKKMNDEHVDTAIMEVSSHALSLGRVHGCDYDIAV 202

Query: 449 FTNLTPESNLDFQESEEEYRDAKAKLFRRMVDPDRHRK----VVNIDDPNAPFFIGQGNP 508
           FTNLT + +LD+ ++ E+YR AK+ LF ++     H K    V+N DD  + +F  +   
Sbjct: 203 FTNLT-QDHLDYHKTMEDYRQAKSLLFSQLGGSFNHEKPKRAVLNADDKASAYF--EKVT 262

Query: 509 DVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQGILEISSGLLGKHNIYNILAAVSV 568
              ++T+ +EN +A V   + E+S   T   + TP+G  +I+  L+G+ N+YN+LAA + 
Sbjct: 263 AAHILTYGIEN-DADVMAKQIEISAQGTSFELVTPKGTKQITVSLVGRFNVYNVLAAAAT 322

Query: 569 GIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGVIVDRAQTPDGLSRLLDSVRELGPR 628
           GIA G P + I   +EE+  V GRFEL++  Q F V+VD A TPD L  +L++ +++   
Sbjct: 323 GIAAGLPFDTITSALEELQGVRGRFELVNHNQPFPVVVDYAHTPDSLENVLNTCKDMTEG 382

Query: 629 RIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSDNPRNEDPLDILDDMLAGIGWTMQD 688
           ++  V GC G+ D+ KRP M +IA   +D  I TSDNPR+EDPL IL DM  G+      
Sbjct: 383 KLFVVVGCGGDRDKTKRPKMAEIAVRIADEPIFTSDNPRSEDPLAILRDMERGV------ 442

Query: 689 YLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMGEEGDVVVVAGKGHETYQIEGDKKD 748
                E  YY  + N         R  A+   +A  ++GDVV++AGKGHETYQ  G++  
Sbjct: 443 -----EGTYYHSIAN---------REQAIFFAIANAKKGDVVLIAGKGHETYQQIGNETF 484

Query: 749 FFDDREECREAL 757
            FDD E    A+
Sbjct: 503 DFDDAEVAGRAI 484

BLAST of Cp4.1LG05g03350 vs. Swiss-Prot
Match: MURE_BACSU (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase OS=Bacillus subtilis (strain 168) GN=murE PE=3 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.5e-88
Identity = 199/515 (38.64%), Postives = 296/515 (57.48%), Query Frame = 1

Query: 246 MTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLYLTEADK 305
           M L +LL      P SV  + + EIT I+ DSR V  G LFVC  G   DGH +  +A +
Sbjct: 1   MKLTKLLTYLTTEP-SVNDSQDPEITSIEMDSREVKKGSLFVCVKGYTVDGHDFAQKAVE 60

Query: 306 RGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVTGTDGKT 365
            GA A+VA +E+D+   +     ++V  +   L  L+ +FY  P+K + +IG+TGT+GKT
Sbjct: 61  NGAAAIVAEREVDVNVPV-----IIVRQSLRALSVLSDAFYGQPTKKLQLIGITGTNGKT 120

Query: 366 STSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLHNGTEAV 425
           ST++++  + +  G RTGL+GT+ Y   GD  L   +TTP+++ +Q    KM     +  
Sbjct: 121 STTHMVDEILKKAGKRTGLIGTM-YMKIGDETLPVKNTTPESVTLQKTFKKMNDKHVDTA 180

Query: 426 VMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMVDPDRH- 485
           +MEVS+  L+ GR    D+DIAVFTNLT + +LD+ ++ +EYR AK+ LF ++     H 
Sbjct: 181 IMEVSSHALSLGRVHGCDYDIAVFTNLT-QDHLDYHKTMDEYRHAKSLLFSQLGGAFNHE 240

Query: 486 ---RKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQG 545
              R V+N DD  + +F  +      + T+ ++N +A V      ++   T   + T +G
Sbjct: 241 HPKRAVLNADDEASAYF--EKVTAAHISTYGIKN-DADVMAKNISITAQGTSFDLVTNKG 300

Query: 546 ILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGVI 605
              I+  L+G+ N+YN+LAAV+  IA G P E I   +EE+  V GRFEL++++Q F VI
Sbjct: 301 TKHITMSLVGQFNVYNVLAAVATCIAAGIPFEIITEAVEELHGVRGRFELVNQQQEFPVI 360

Query: 606 VDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSDN 665
           VD A TPD L  +L++ R++   ++  V GC G+ D+ KRP M KIA + +D  I TSDN
Sbjct: 361 VDYAHTPDSLENVLETCRDMTEGKLFVVVGCGGDRDKTKRPKMAKIAVELADEPIFTSDN 420

Query: 666 PRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMGE 725
           PR+EDP  IL DM AG+           EN YY  + N         R  A+   +A  +
Sbjct: 421 PRSEDPRAILRDMEAGV-----------ENAYYHSIAN---------REQAIFFAIANAK 480

Query: 726 EGDVVVVAGKGHETYQIEGDKKDFFDDREECREAL 757
           +GDVV++AGKGHETYQ  G++   FDD E    A+
Sbjct: 481 KGDVVLIAGKGHETYQQIGNETFDFDDAEVAARAI 484

BLAST of Cp4.1LG05g03350 vs. Swiss-Prot
Match: MURE_SYNWW (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase OS=Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen) GN=murE PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 4.3e-88
Identity = 194/494 (39.27%), Postives = 292/494 (59.11%), Query Frame = 1

Query: 264 GNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLYLTEADKRGAVAVVASKEIDIEETL 323
           G L+ EI+GI +DSR V  G LF+C  G ++DGHL+  +A + GAVAV+A +++D   T 
Sbjct: 17  GPLDREISGIAYDSRRVKPGDLFICISGLKSDGHLFAGQAIENGAVAVLAERQLD---TG 76

Query: 324 GCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVTGTDGKTSTSYLIKGMYEAMGLRTG 383
           G   L+   DT S L  LAA++Y  PSK++ V+ VTGT+GKT+T+ LIK + E  G +T 
Sbjct: 77  GKATLLTTPDTRSALALLAANYYGRPSKSIRVVAVTGTNGKTTTTDLIKAILEEAGKKTA 136

Query: 384 LLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLHNGTEAVVMEVSTAELARGRCDEVD 443
           ++GT+ Y   G+ + E   TTP+A+ +++ MA       + +VMEVS+  L   R  E+D
Sbjct: 137 IMGTL-YAQVGEIQREMQHTTPEALEIESFMALCREEKADYIVMEVSSHALQLQRVAEID 196

Query: 444 FDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMVDPDRHRKVVNIDDPNAPFFIGQGN 503
           F++AVFTNLT + +LDF ++ + YR AK +LF+ + +  ++  ++NIDDP A        
Sbjct: 197 FNVAVFTNLT-QDHLDFHQNMDNYRAAKLQLFQMIKEEKQNYAIINIDDPWAEEIFQAAT 256

Query: 504 PDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQGILEISSGLLGKHNIYNILAAVS 563
             +P  ++ ++ K +     + ++ L  +   +H     L I+  L+G  +IYN LAA+S
Sbjct: 257 --IPCRSYGIK-KRSDYQAGELKIDLDGSSFRLHYGDNSLMINMKLIGLFSIYNALAAIS 316

Query: 564 VGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGVIVDRAQTPDGLSRLLDSVRELGP 623
             +  G     I   +  V+ VPGRFE +D  Q F VIVD A TPDGL  +L + RELG 
Sbjct: 317 FALQEGIDPGLIQSALARVEGVPGRFEKVDCGQDFAVIVDYAHTPDGLENILQTSRELGK 376

Query: 624 RRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSDNPRNEDPLDILDDMLAGIGWTMQ 683
           +R+I VFGC G+ DR KRP+M +IA   SD  ++TSDNPR+EDP  I+ +++ G      
Sbjct: 377 KRLICVFGCGGDRDRSKRPLMGEIAAKYSDFCVVTSDNPRSEDPHAIIAEIIPG------ 436

Query: 684 DYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMGEEGDVVVVAGKGHETYQIEGDKK 743
             +   E   Y  + +         RR A+R  + +   GD+VV+AGKGHE YQ+  D+ 
Sbjct: 437 --MDKVEKSRYAIIVD---------RREAIRHAIHLARTGDLVVIAGKGHENYQLVKDQV 485

Query: 744 DFFDDREECREALQ 758
             FDDR+   E L+
Sbjct: 497 LEFDDRKVAAELLR 485

BLAST of Cp4.1LG05g03350 vs. Swiss-Prot
Match: MURE_GEOKA (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase OS=Geobacillus kaustophilus (strain HTA426) GN=murE PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 9.6e-88
Identity = 190/493 (38.54%), Postives = 284/493 (57.61%), Query Frame = 1

Query: 269 EITGIQHDSRMVNAGYLFVCCVGRETDGHLYLTEADKRGAVAVVASKEIDIEETLGCKAL 328
           +I  ++ DSR V +G LF C  G   DGH +  EA  RGA A+VA + + ++  +     
Sbjct: 22  DIVALEMDSRRVASGSLFFCLKGFTVDGHDFAEEAVARGAAAIVAERPLSVDVPV----- 81

Query: 329 VMVEDTNSVLPALAASFYMNPSKNMAVIGVTGTDGKTSTSYLIKGMYEAMGLRTGLLGTV 388
           V+V D+   +  LA +FY  P+  + +IGVTGT+GKT+T+ +I+ +    G +TGL+GTV
Sbjct: 82  VLVSDSRRAMAILADAFYGRPTHRLHLIGVTGTNGKTTTTSIIEQIARKTGKKTGLIGTV 141

Query: 389 AYYLHGDNKLEAPSTTPDAILVQNLMAKMLHNGTEAVVMEVSTAELARGRCDEVDFDIAV 448
              + GD    A +TTP+++++Q    +M+  G E V MEVS+  L +GR    D+D+AV
Sbjct: 142 HIKV-GDRSYPAANTTPESLILQRTFKQMVDEGVEFVAMEVSSHALHQGRVHGCDYDVAV 201

Query: 449 FTNLTPESNLDFQESEEEYRDAKAKLFRRMVDPDRHRK----VVNIDDPNAPFFIGQGNP 508
           FTNLT + +LD+  + EEYR+AK  LF ++ +    R+    V+N DDP + ++  +   
Sbjct: 202 FTNLT-QDHLDYHGTMEEYRNAKGLLFAQLGNRYDERRPKFAVLNHDDPVSQYY--KHMT 261

Query: 509 DVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQGILEISSGLLGKHNIYNILAAVSV 568
             P+VT+ M  K+  +   +  ++       + TP G   + + L+G  N+YNILAA + 
Sbjct: 262 AAPIVTYGMREKSDVMAE-QIRMTAGGMAFRLCTPHGSAAVETKLVGSFNVYNILAAAAA 321

Query: 569 GIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGVIVDRAQTPDGLSRLLDSVRELGPR 628
            +A G  LE I   + +V+ VPGRFE +DE Q F +IVD A TPD L   L +VR+L  R
Sbjct: 322 CLASGFSLETIAAALADVEPVPGRFETVDEGQNFTIIVDYAHTPDSLENALKTVRQLAKR 381

Query: 629 RIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSDNPRNEDPLDILDDMLAGIGWTMQD 688
            +  V GC G+ D  KRP+M ++A   +D+ I TSDNPR+EDP  IL DM AG+   +  
Sbjct: 382 NVYVVIGCGGDRDPSKRPLMAQVAVRYADVAIFTSDNPRSEDPKQILRDMEAGVSAEI-- 441

Query: 689 YLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMGEEGDVVVVAGKGHETYQIEGDKKD 748
                          G  + + D R  A+R  +   +EGDVV++AGKGHETYQI G+   
Sbjct: 442 ---------------GKHVTIPD-REEAIRYAIGQAQEGDVVLIAGKGHETYQIIGNDVI 486

Query: 749 FFDDREECREALQ 758
            FDDR   R A++
Sbjct: 502 EFDDRAVARAAVK 486

BLAST of Cp4.1LG05g03350 vs. TrEMBL
Match: A0A0A0KMJ8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G153040 PE=3 SV=1)

HSP 1 Score: 1406.3 bits (3639), Expect = 0.0e+00
Identity = 714/779 (91.66%), Postives = 740/779 (94.99%), Query Frame = 1

Query: 1   MASTFTFLPFPLILSPRPSIPCVRPQFRRQYFPTIRILRPSVPSAIGPDGKFYPNPADDD 60
           MASTFTFLPFPLILSPR + P +RPQF RQY P IRILRPSVPSAIGPDGKFYP+PADDD
Sbjct: 1   MASTFTFLPFPLILSPRSNFPFIRPQFHRQYLPAIRILRPSVPSAIGPDGKFYPDPADDD 60

Query: 61  PPEAPEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAEYLNSE 120
           PPEAPED+GHGVSKFQQIYRQAARA+KIQEEDFKKHQSTYLSAIADVEDAP+NAEYLNSE
Sbjct: 61  PPEAPEDSGHGVSKFQQIYRQAARARKIQEEDFKKHQSTYLSAIADVEDAPENAEYLNSE 120

Query: 121 SSGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDKDILEGIDELETEEVVDLEEIN 180
           SSGDDLFGEIDKAIA+KRKEFVKQGLLKPNPKKEK  +KD +EGIDELETEEV DLEEIN
Sbjct: 121 SSGDDLFGEIDKAIALKRKEFVKQGLLKPNPKKEKVGEKDEIEGIDELETEEVADLEEIN 180

Query: 181 ELQGLTEISEDENEDKDGDEEGPGNLDDDARELHGSDELSSFNSFDVDFDSYGKVKTRIV 240
           EL+GLT ISED + ++D DE+GP NLDD+   + G DELSSFNSFDVDFDSYGKVK RIV
Sbjct: 181 ELRGLTVISEDVDVEEDEDEDGPRNLDDNVSAIGGEDELSSFNSFDVDFDSYGKVKARIV 240

Query: 241 EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLYL 300
           EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSR+V AG LFVCCVGRETDGHLYL
Sbjct: 241 EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRVVTAGDLFVCCVGRETDGHLYL 300

Query: 301 TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVTG 360
           TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFY NPSKNMAVIG+TG
Sbjct: 301 TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYRNPSKNMAVIGITG 360

Query: 361 TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLHN 420
           TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLE PSTTPDAILVQNLMAKMLHN
Sbjct: 361 TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLELPSTTPDAILVQNLMAKMLHN 420

Query: 421 GTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMVD 480
           GTEAVVMEVS+  LARGRCDEVDFDIAVFTNLT   +LDFQ SEEEYRDAKAKLF+RMVD
Sbjct: 421 GTEAVVMEVSSDGLARGRCDEVDFDIAVFTNLT-RDHLDFQGSEEEYRDAKAKLFKRMVD 480

Query: 481 PDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQ 540
           PDRHRKV+NIDDPNAPFFIGQGNPDVPVVTFAMENKNA VHPLKYELSLFETQVLVHTPQ
Sbjct: 481 PDRHRKVINIDDPNAPFFIGQGNPDVPVVTFAMENKNADVHPLKYELSLFETQVLVHTPQ 540

Query: 541 GILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGV 600
           GILEISSGLLGKHNIYNILAAV+VGIAVGAPLEDIVRG+EEVDAVPGRFELIDEEQAFGV
Sbjct: 541 GILEISSGLLGKHNIYNILAAVAVGIAVGAPLEDIVRGVEEVDAVPGRFELIDEEQAFGV 600

Query: 601 IVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSD 660
           IVD A TPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSD+TILTSD
Sbjct: 601 IVDHAHTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDVTILTSD 660

Query: 661 NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMG 720
           NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPL NGHRIFLHDIRRVAVRA VAMG
Sbjct: 661 NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLSNGHRIFLHDIRRVAVRAAVAMG 720

Query: 721 EEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSEFPWRCVPE 780
           EEGDVVVVAGKGHETYQIE DK DFFDDREECREALQYVDELHQAGIDTSEFPWR +PE
Sbjct: 721 EEGDVVVVAGKGHETYQIEDDKTDFFDDREECREALQYVDELHQAGIDTSEFPWR-LPE 777

BLAST of Cp4.1LG05g03350 vs. TrEMBL
Match: M5X0D3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001774mg PE=3 SV=1)

HSP 1 Score: 1186.8 bits (3069), Expect = 0.0e+00
Identity = 610/780 (78.21%), Postives = 682/780 (87.44%), Query Frame = 1

Query: 5   FTFLPFPLILSPRPSIPCVRPQFR--RQYFPTI--RILRPSVPSAIGPDGKFYPNPADDD 64
           FTFL  P  LSP+P+   ++P F   R   P++  R++RP+   AIGPDGK+YP+PADDD
Sbjct: 3   FTFLSIPHFLSPQPNFLSLKPNFTSLRPSIPSLSARLIRPT--HAIGPDGKYYPDPADDD 62

Query: 65  PPEAPEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAEYLNSE 124
           PPEAPED+GHGVSKFQQI RQA+R +K+QEEDFKKHQ T+L+AIADVED P+N+  + +E
Sbjct: 63  PPEAPEDSGHGVSKFQQIQRQASRHRKLQEEDFKKHQDTFLNAIADVEDPPENSSSVTNE 122

Query: 125 SSGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDKDILEGIDELETEEVVDLEEIN 184
           +SGDDLFG+ID AIA+KRKEFVKQGLLKPNPKKE       +  +DEL+ EEVVDLEEI+
Sbjct: 123 NSGDDLFGDIDHAIALKRKEFVKQGLLKPNPKKE-------IVAVDELDPEEVVDLEEID 182

Query: 185 ELQGLTEISEDENEDKDGDEEGPGNLDDDARELHGSDELSSFNS-FDVDFDSYGKVKTRI 244
           ELQGL  +SED       DE+GP   D    +L G D     NS FD+DFDSYGK + RI
Sbjct: 183 ELQGLRVVSEDL------DEDGPEKFDSKVSDLDGKDGNLRLNSEFDLDFDSYGKTRARI 242

Query: 245 VEPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLY 304
           VEPKFKM+LAELLDESKVVPVSV+G+LE+EITGIQHDSR+VN+G LFVCCVG +TDGHLY
Sbjct: 243 VEPKFKMSLAELLDESKVVPVSVYGDLEVEITGIQHDSRVVNSGDLFVCCVGSKTDGHLY 302

Query: 305 LTEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVT 364
           L+EA KRGAVAVVASKEI +EE LGCKALV+VEDTN+ LPALAASFY  PSKNMAVIG+T
Sbjct: 303 LSEAIKRGAVAVVASKEIYMEENLGCKALVIVEDTNADLPALAASFYRYPSKNMAVIGIT 362

Query: 365 GTDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLH 424
           GT+GKT+T+YLIKG+YEAMGLRTG+L TVAYY+HGDNKLE+P+TTPDA+LVQNLMAKMLH
Sbjct: 363 GTNGKTTTAYLIKGLYEAMGLRTGMLSTVAYYVHGDNKLESPNTTPDAVLVQNLMAKMLH 422

Query: 425 NGTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMV 484
           NG EAVVME S+  LA  RCDEVDFDIAVFTNLT + +LDF ++EEEYRDAKAKLF RMV
Sbjct: 423 NGAEAVVMEASSHGLALARCDEVDFDIAVFTNLTRD-HLDFHKTEEEYRDAKAKLFSRMV 482

Query: 485 DPDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTP 544
           DP+RHRKVVNIDDPNA FFI QGNPD+PVVTFAMENKNA VHPLK+ELSLFETQVLV TP
Sbjct: 483 DPERHRKVVNIDDPNATFFIAQGNPDIPVVTFAMENKNADVHPLKFELSLFETQVLVSTP 542

Query: 545 QGILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFG 604
           QGILEISSGLLG+HNIYNILAAV+VGIAVGAPLEDIVRGIEEVDAVPGR ELIDEEQAFG
Sbjct: 543 QGILEISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVRGIEEVDAVPGRCELIDEEQAFG 602

Query: 605 VIVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTS 664
           VIVD A TPD LSRLLDSVRELG RRII+V GC GE DRGKRPMMTKIATDKSD+TILTS
Sbjct: 603 VIVDYAHTPDALSRLLDSVRELGARRIISVIGCPGESDRGKRPMMTKIATDKSDVTILTS 662

Query: 665 DNPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAM 724
           DNP+NEDPLDILDDMLAG+GWTMQDYLKHGENDYYPPLPNGHR+FLHDIRRVAVR  VAM
Sbjct: 663 DNPKNEDPLDILDDMLAGVGWTMQDYLKHGENDYYPPLPNGHRLFLHDIRRVAVRCAVAM 722

Query: 725 GEEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSEFPWRCVPE 780
           GEEGD+VVVAGKGHE YQIEGDKK+FFDDREECREALQYVDELHQAGIDTSEFPWR +PE
Sbjct: 723 GEEGDMVVVAGKGHEAYQIEGDKKEFFDDREECREALQYVDELHQAGIDTSEFPWR-LPE 765

BLAST of Cp4.1LG05g03350 vs. TrEMBL
Match: F6HUC1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03410 PE=3 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 606/788 (76.90%), Postives = 684/788 (86.80%), Query Frame = 1

Query: 1   MASTFTFLPFPLILSPRPSIPCVRP---QFR--RQYFPTIRILRPSVPSAIGPDGKFYPN 60
           MA TF  L  P +    P   C++P   QF+    +FP  R LR   P+ +   G F PN
Sbjct: 1   MALTFFSLTTPFMY---PHSLCLKPSISQFKSLHGFFPLHRRLRS--PAVVVSAGNFDPN 60

Query: 61  PADDDPPEAPEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAE 120
           PADDDPPEAPED+ HG+ KFQQI RQAARA+K +EE FKK QST+L+AIADVEDAPDN +
Sbjct: 61  PADDDPPEAPEDSAHGLFKFQQIQRQAARARKREEEQFKKDQSTFLNAIADVEDAPDNVD 120

Query: 121 YLNSESSGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDKDILEGIDELETEEVVD 180
            +++E +GDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKE  + ++I EGI+ELE +EVVD
Sbjct: 121 SIDAEGAGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKESVVPEEIAEGIEELEPDEVVD 180

Query: 181 LEEINELQGLTEISED----ENEDKDGDEEGPGNLDDDARELHGSDELSSFNSFDVDFDS 240
           LEEINEL+GLT ISED    E+E  D +E   GN++  +         SS +SFDVDFDS
Sbjct: 181 LEEINELRGLTVISEDLDDEESEKLDDEESDSGNVNGGS---------SSHSSFDVDFDS 240

Query: 241 YGKVKTRIVEPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVG 300
           +G+   RIVEPKF+M+LAELLDESKVVPVSV+G+LE+EITGIQHDSR+V++G LFVCCVG
Sbjct: 241 FGESGVRIVEPKFRMSLAELLDESKVVPVSVYGDLEVEITGIQHDSRVVSSGDLFVCCVG 300

Query: 301 RETDGHLYLTEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSK 360
           R+TDGHLYL+EADKRGAVAVVASKEIDIEETLGCKALV+VEDT+ VLPALAA+FY +PSK
Sbjct: 301 RKTDGHLYLSEADKRGAVAVVASKEIDIEETLGCKALVIVEDTSLVLPALAAAFYGHPSK 360

Query: 361 NMAVIGVTGTDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQ 420
           NMAVIG+ GT+GKT+T+YLI+ MYEAMGLRTG+L TVAYY+HG NKLEAP TTPDA+L+Q
Sbjct: 361 NMAVIGIVGTNGKTTTAYLIRAMYEAMGLRTGMLSTVAYYVHGKNKLEAPYTTPDAVLIQ 420

Query: 421 NLMAKMLHNGTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAK 480
            LMAKMLHNGTEAVVME S+  LA GRC+EVDFDIAVFTNLT + ++DF ESEE+YR+AK
Sbjct: 421 KLMAKMLHNGTEAVVMEASSHGLALGRCNEVDFDIAVFTNLTRD-HMDFHESEEDYRNAK 480

Query: 481 AKLFRRMVDPDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFE 540
           AKLF RMVDPDRHRK+VNIDDPNAPFFI QGNPDVPVVTFAMENK+A VHPLK+ELSLFE
Sbjct: 481 AKLFSRMVDPDRHRKIVNIDDPNAPFFIAQGNPDVPVVTFAMENKDADVHPLKFELSLFE 540

Query: 541 TQVLVHTPQGILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFEL 600
           TQVLV TP+GILEISSGLLG+HNIYNILAAV+VGIAVGAPLEDIVRGIEE+DAVPGR EL
Sbjct: 541 TQVLVQTPKGILEISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVRGIEEIDAVPGRCEL 600

Query: 601 IDEEQAFGVIVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDK 660
           IDEEQAFGVIVD A TPD LSRLLDSVRELG RRIITVFGC GE DRGKRP+MTKIATDK
Sbjct: 601 IDEEQAFGVIVDYAHTPDALSRLLDSVRELGARRIITVFGCAGESDRGKRPIMTKIATDK 660

Query: 661 SDITILTSDNPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRV 720
           SD+ ILTSDNP+NEDPLDILDDMLAG+GWTM DYLK+G NDYYPPLPNGHR+FLHDIRRV
Sbjct: 661 SDVVILTSDNPKNEDPLDILDDMLAGVGWTMHDYLKYGANDYYPPLPNGHRLFLHDIRRV 720

Query: 721 AVRAGVAMGEEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSE 780
           AVRA VAMGEEGD+VVVAGKGHETYQIEGDKK+FFDDREECREALQYVDELHQAGIDTSE
Sbjct: 721 AVRAAVAMGEEGDIVVVAGKGHETYQIEGDKKEFFDDREECREALQYVDELHQAGIDTSE 772

BLAST of Cp4.1LG05g03350 vs. TrEMBL
Match: W9QPL2_9ROSA (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase OS=Morus notabilis GN=L484_024268 PE=3 SV=1)

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 601/777 (77.35%), Postives = 673/777 (86.62%), Query Frame = 1

Query: 5   FTFLPFPLILSPRPSIPCVRPQFRRQYFPTIRILRPSVP---SAIGPDGKFYPNPADDDP 64
           F FL  P +L+ +P  P + P+    +     +  PS P   SAIGPDGKFYP+PADDDP
Sbjct: 3   FAFLSSPHLLTAQP--PFLSPKRHHSFLKPRPLSLPSRPLPLSAIGPDGKFYPDPADDDP 62

Query: 65  PEAPEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAEYLNSES 124
           PE PEDAGHGVSKFQQI RQA+RA+K++EE+FKKHQST+LSAIADVED P+    +  ES
Sbjct: 63  PEVPEDAGHGVSKFQQIERQASRARKLEEEEFKKHQSTFLSAIADVEDPPETPRSIYDES 122

Query: 125 SGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDKDILEGIDELETEEVVDLEEINE 184
           SG DLFGEIDKAIA+KRKEFVKQGLLKPNPKKE       +E I EL+ EEV DLEEI+E
Sbjct: 123 SGGDLFGEIDKAIALKRKEFVKQGLLKPNPKKED------VEVIGELDPEEVDDLEEIDE 182

Query: 185 LQGLTEISEDENEDKDGDEEGPGNLDDDARELHGSDELSSF-NSFDVDFDSYGKVKTRIV 244
           LQGLT  S+D + D   D E   N DD+  E   +D  SSF +SFD+DFDSY K   RIV
Sbjct: 183 LQGLTVGSDDADSD---DSE---NFDDEVGEFSSNDRNSSFGSSFDLDFDSYDKSMARIV 242

Query: 245 EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLYL 304
           EPKFK++LAELLDESKVVPVSV+G+LE+EITGIQHDSR+V++G LFVCCVGR+TDGHLYL
Sbjct: 243 EPKFKISLAELLDESKVVPVSVYGDLEVEITGIQHDSRLVSSGDLFVCCVGRKTDGHLYL 302

Query: 305 TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVTG 364
           +EADKRGAVAVVASKEIDIEETLGCKALV+VEDT++VLP LAA+FY  PSKNMAVIG+TG
Sbjct: 303 SEADKRGAVAVVASKEIDIEETLGCKALVIVEDTSAVLPTLAAAFYRFPSKNMAVIGITG 362

Query: 365 TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLHN 424
           T+GKT+T+YLIK MYE+MGLRTG+L TVAYY+HGDNKLE+P+TTPDA+LVQNLMAKMLHN
Sbjct: 363 TNGKTTTTYLIKSMYESMGLRTGMLSTVAYYVHGDNKLESPNTTPDAVLVQNLMAKMLHN 422

Query: 425 GTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMVD 484
           G EAVVME S+  LA GRC+EVDFDIAVFTNLT + +LDF  SEEEYRDAKAKLF RMVD
Sbjct: 423 GAEAVVMEASSHGLALGRCNEVDFDIAVFTNLTRD-HLDFHGSEEEYRDAKAKLFARMVD 482

Query: 485 PDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQ 544
           P+RHRKVVNIDDPNA FFI QGN DVPV+T+A++ K A VHPLK+ELSLFETQVLV+TPQ
Sbjct: 483 PERHRKVVNIDDPNASFFIAQGNLDVPVITYAIDYKKADVHPLKFELSLFETQVLVNTPQ 542

Query: 545 GILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGV 604
           GILEISSGLLG+HNIYNILAAV+VGIAVGAPLE+IVRGIEEVDAVPGR ELIDEEQAFGV
Sbjct: 543 GILEISSGLLGRHNIYNILAAVAVGIAVGAPLENIVRGIEEVDAVPGRCELIDEEQAFGV 602

Query: 605 IVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSD 664
           IVD A TPD LSRLLDSVRELG RRIITV GC GE DRGKRPMM KIATDKSD+TILTSD
Sbjct: 603 IVDYAHTPDALSRLLDSVRELGARRIITVIGCGGERDRGKRPMMGKIATDKSDVTILTSD 662

Query: 665 NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMG 724
           N RNEDPLDILDDMLAG+GWTMQ+YLK+GENDYYPPLPNGHR+FLHDIRRVAVR  VAMG
Sbjct: 663 NSRNEDPLDILDDMLAGVGWTMQEYLKYGENDYYPPLPNGHRLFLHDIRRVAVRCAVAMG 722

Query: 725 EEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSEFPWRCV 778
           EEGDVVV+AGKGHETY+IEGDKK+FFDDREECREALQYVDELHQAGIDTSEFPWR V
Sbjct: 723 EEGDVVVIAGKGHETYEIEGDKKEFFDDREECREALQYVDELHQAGIDTSEFPWRLV 764

BLAST of Cp4.1LG05g03350 vs. TrEMBL
Match: A0A059CGL5_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01840 PE=3 SV=1)

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 602/800 (75.25%), Postives = 685/800 (85.62%), Query Frame = 1

Query: 1   MASTFTFLPFPLILSPR-PSIPCVRPQFRRQYFP-----------TIRILRPSVPSAIGP 60
           M+ + T  P P  LSP+ PS   + P  RR++             ++R   P  P A+ P
Sbjct: 1   MSLSVTSFPLPRSLSPQVPSFLSLNPGRRRRFASGGHRRPPLLLLSVRRPGPGPPHALAP 60

Query: 61  DGKFYPNPADDDPPEAPEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVE 120
           DGK YP+PADDDPPEAPEDA HGVSKFQQI+RQA+RA++I+E+DFKKH+STYL+AIADVE
Sbjct: 61  DGKSYPDPADDDPPEAPEDAEHGVSKFQQIHRQASRARRIEEDDFKKHRSTYLAAIADVE 120

Query: 121 DAPDNAEYL-----NSESSGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDKDILE 180
           DAP++         ++  +GDDLFGEIDKAIA+KR+EFVKQGLLKPN KK KA D     
Sbjct: 121 DAPEDGGDAPGGGQSAGDAGDDLFGEIDKAIALKRREFVKQGLLKPNSKKGKADD----- 180

Query: 181 GIDELETEEVVDLEEINELQGLTEISEDENEDKDG----DEEGPGNLDDDARELHGSDEL 240
            ++EL+ EEVVDLEEI ELQGL  +SE ++ D DG    D+E  GNL   +R   GS   
Sbjct: 181 AVEELQPEEVVDLEEIQELQGLRVVSEGDDAD-DGAGEFDDEVSGNL---SRSNGGS--- 240

Query: 241 SSFNSFDVDFDSYGKVKTRIVEPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRM 300
            S +SFD+DFD+YG+ K RIVEPKF++TLAELLDESKVVPVSV+GNLE+EITGIQHDSR+
Sbjct: 241 LSDSSFDLDFDAYGRTKARIVEPKFRITLAELLDESKVVPVSVYGNLEVEITGIQHDSRV 300

Query: 301 VNAGYLFVCCVGRETDGHLYLTEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLP 360
           V++G LFVCCVGR+TDGHL+L+EADKRGAVAVVASKEIDIEETLGCKALV+VEDTN+VLP
Sbjct: 301 VSSGDLFVCCVGRKTDGHLFLSEADKRGAVAVVASKEIDIEETLGCKALVIVEDTNAVLP 360

Query: 361 ALAASFYMNPSKNMAVIGVTGTDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLE 420
           ALAASFY  PS++MAVIG+TGT GKT+TSYLIKGMYEAMGLRTG+L TVAYY+H DNKLE
Sbjct: 361 ALAASFYRQPSRHMAVIGITGTYGKTTTSYLIKGMYEAMGLRTGMLSTVAYYVHRDNKLE 420

Query: 421 APSTTPDAILVQNLMAKMLHNGTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLD 480
            P+T PDA+LVQNLMAKMLHNGTEA+ MEVS+  LA GR +EVDFDIAVFTNLT + NLD
Sbjct: 421 LPNTEPDAVLVQNLMAKMLHNGTEAIAMEVSSHGLAVGRYNEVDFDIAVFTNLTHD-NLD 480

Query: 481 FQESEEEYRDAKAKLFRRMVDPDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAA 540
           F  + EEY+ A+AKLF RMVDP+RHRK+VNIDDPNAPFF+ QGNPDVPVVTFAMENKNA 
Sbjct: 481 FHGTREEYKAAEAKLFSRMVDPERHRKIVNIDDPNAPFFMAQGNPDVPVVTFAMENKNAD 540

Query: 541 VHPLKYELSLFETQVLVHTPQGILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGI 600
           VHPLK+ELSLFETQVLV+TP GILEISSGLLG+HNIYNILAAV+VGIAVGAPLEDIVRGI
Sbjct: 541 VHPLKFELSLFETQVLVNTPNGILEISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVRGI 600

Query: 601 EEVDAVPGRFELIDEEQAFGVIVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRG 660
           EEVDAVPGRFELIDEEQAFGVIVD A TPD LSRLLDS RELGPRRIITV GC GE DRG
Sbjct: 601 EEVDAVPGRFELIDEEQAFGVIVDYAHTPDALSRLLDSARELGPRRIITVIGCAGESDRG 660

Query: 661 KRPMMTKIATDKSDITILTSDNPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPN 720
            RPMMTKIATDKS++T+LTSDNP+NEDPLDILDDMLAG+GWTMQDYLK+GENDYYPPLPN
Sbjct: 661 NRPMMTKIATDKSEVTMLTSDNPKNEDPLDILDDMLAGVGWTMQDYLKYGENDYYPPLPN 720

Query: 721 GHRIFLHDIRRVAVRAGVAMGEEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYV 780
           GHR+FLHDIRRVAVRA VAMGEEGD+VVVAGKGHE+YQ+EGDKK+FFDDREECREALQYV
Sbjct: 721 GHRLFLHDIRRVAVRAAVAMGEEGDMVVVAGKGHESYQMEGDKKEFFDDREECREALQYV 780

BLAST of Cp4.1LG05g03350 vs. TAIR10
Match: AT1G63680.1 (AT1G63680.1 acid-amino acid ligases;ligases;ATP binding;ATP binding;ligases)

HSP 1 Score: 1063.9 bits (2750), Expect = 4.7e-311
Identity = 561/788 (71.19%), Postives = 657/788 (83.38%), Query Frame = 1

Query: 5   FTFL-PFPLILSPRPSIPCVRPQFRRQYFPTIRILRPSVPSAIGPDGKFYPNPADDDPPE 64
           FTFL P P+ LS   +       ++    P  R  R    +A       YPNPADDDPPE
Sbjct: 3   FTFLSPHPVFLSLTGTTSSF--SYKPVLLPFSRNSRTLTVAAGPARRNSYPNPADDDPPE 62

Query: 65  APEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAEYLNSESSG 124
           APED+ HGVSKFQQI RQAARA+K++EEDF+K+++TYLSAIADVEDA +     + E SG
Sbjct: 63  APEDSMHGVSKFQQIQRQAARARKLEEEDFEKNRNTYLSAIADVEDAAETGR--DDEESG 122

Query: 125 DDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDK----------DILEGIDELETEEV 184
            DLF +ID+AI+MKR EFVKQGLLKPNP K  ++ K          D+ + +DEL+ EEV
Sbjct: 123 GDLFSDIDRAISMKRSEFVKQGLLKPNPPKTASLKKIGEEGNEEEGDVTDDVDELDEEEV 182

Query: 185 VDLEEINELQGLTEISEDENEDKDGDEEGPGNLDDDARELHGSDELSSFNSFDVDFDSYG 244
           VDL+EI++L GLTEIS++E+     DEE  GN   + ++  GSD     + F+ D D +G
Sbjct: 183 VDLDEIDKLTGLTEISDEEDW---VDEE--GNTRINKKKEFGSD-----HQFEFDLDDFG 242

Query: 245 KVKTRIVEPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRE 304
           + K RIVEPKFKM LAELLDESKVVP+SV+G+L++EITGIQHDSR V+AG LFVCC+G E
Sbjct: 243 ESKARIVEPKFKMCLAELLDESKVVPISVYGDLDVEITGIQHDSRGVSAGDLFVCCLGSE 302

Query: 305 TDGHLYLTEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNM 364
                +L+EADKRGAVAVVASKEIDIE+TLGC+ALV+VEDTN+VL ALA+SFY +PSKNM
Sbjct: 303 N----FLSEADKRGAVAVVASKEIDIEDTLGCRALVIVEDTNAVLAALASSFYRHPSKNM 362

Query: 365 AVIGVTGTDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTT--PDAILVQ 424
           +VIGVTGTDGKT+T+YLIK +YEAMG+RTG+  TV+ Y+HGDNKL+ P+ T  PDA+LVQ
Sbjct: 363 SVIGVTGTDGKTTTTYLIKSLYEAMGVRTGMFSTVSCYIHGDNKLDTPNATMNPDAVLVQ 422

Query: 425 NLMAKMLHNGTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAK 484
           +LMAKMLHNGTE++VME S  ELA G+CDEVDFDIAVFTNLT E N DF+ ++EEYRDA+
Sbjct: 423 SLMAKMLHNGTESLVMEASPQELALGKCDEVDFDIAVFTNLTRE-NTDFRGTDEEYRDAE 482

Query: 485 AKLFRRMVDPDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFE 544
           AKLF RMVDP+RHRKVVNIDDPNA FF+ QGNP+VPVVTFAMEN  A VHPLK+ELSLFE
Sbjct: 483 AKLFSRMVDPERHRKVVNIDDPNAAFFVQQGNPNVPVVTFAMENTKADVHPLKFELSLFE 542

Query: 545 TQVLVHTPQGILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFEL 604
           TQVLV+TPQGILEISSGLLG+HNIYNILAAV+VGIAVGAPLEDIVRG+EEVDAVPGR EL
Sbjct: 543 TQVLVNTPQGILEISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVRGVEEVDAVPGRCEL 602

Query: 605 IDEEQAFGVIVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDK 664
           IDEEQAFGVIVD A TPDGLSRLLDS+REL PRRIITV GC GE++RGKRP+MTKIAT+K
Sbjct: 603 IDEEQAFGVIVDHANTPDGLSRLLDSIRELKPRRIITVIGCEGENERGKRPLMTKIATEK 662

Query: 665 SDITILTSDNPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRV 724
           SD+T+LTSDNPRNEDPLDILDDML+GIGWTMQ+YLKHGE+DYYPPL NGHR+FLHDIRRV
Sbjct: 663 SDVTMLTSDNPRNEDPLDILDDMLSGIGWTMQEYLKHGEHDYYPPLANGHRLFLHDIRRV 722

Query: 725 AVRAGVAMGEEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSE 780
           AVR  VAMGEEGD+VVVAGKGHE YQ+EG+KK+F+DDREECREALQYVDELHQAGIDTSE
Sbjct: 723 AVRCAVAMGEEGDMVVVAGKGHEAYQLEGEKKEFYDDREECREALQYVDELHQAGIDTSE 770

BLAST of Cp4.1LG05g03350 vs. NCBI nr
Match: gi|449452306|ref|XP_004143900.1| (PREDICTED: uncharacterized protein LOC101217207 [Cucumis sativus])

HSP 1 Score: 1406.3 bits (3639), Expect = 0.0e+00
Identity = 714/779 (91.66%), Postives = 740/779 (94.99%), Query Frame = 1

Query: 1   MASTFTFLPFPLILSPRPSIPCVRPQFRRQYFPTIRILRPSVPSAIGPDGKFYPNPADDD 60
           MASTFTFLPFPLILSPR + P +RPQF RQY P IRILRPSVPSAIGPDGKFYP+PADDD
Sbjct: 1   MASTFTFLPFPLILSPRSNFPFIRPQFHRQYLPAIRILRPSVPSAIGPDGKFYPDPADDD 60

Query: 61  PPEAPEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAEYLNSE 120
           PPEAPED+GHGVSKFQQIYRQAARA+KIQEEDFKKHQSTYLSAIADVEDAP+NAEYLNSE
Sbjct: 61  PPEAPEDSGHGVSKFQQIYRQAARARKIQEEDFKKHQSTYLSAIADVEDAPENAEYLNSE 120

Query: 121 SSGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDKDILEGIDELETEEVVDLEEIN 180
           SSGDDLFGEIDKAIA+KRKEFVKQGLLKPNPKKEK  +KD +EGIDELETEEV DLEEIN
Sbjct: 121 SSGDDLFGEIDKAIALKRKEFVKQGLLKPNPKKEKVGEKDEIEGIDELETEEVADLEEIN 180

Query: 181 ELQGLTEISEDENEDKDGDEEGPGNLDDDARELHGSDELSSFNSFDVDFDSYGKVKTRIV 240
           EL+GLT ISED + ++D DE+GP NLDD+   + G DELSSFNSFDVDFDSYGKVK RIV
Sbjct: 181 ELRGLTVISEDVDVEEDEDEDGPRNLDDNVSAIGGEDELSSFNSFDVDFDSYGKVKARIV 240

Query: 241 EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLYL 300
           EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSR+V AG LFVCCVGRETDGHLYL
Sbjct: 241 EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRVVTAGDLFVCCVGRETDGHLYL 300

Query: 301 TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVTG 360
           TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFY NPSKNMAVIG+TG
Sbjct: 301 TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYRNPSKNMAVIGITG 360

Query: 361 TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLHN 420
           TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLE PSTTPDAILVQNLMAKMLHN
Sbjct: 361 TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLELPSTTPDAILVQNLMAKMLHN 420

Query: 421 GTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMVD 480
           GTEAVVMEVS+  LARGRCDEVDFDIAVFTNLT   +LDFQ SEEEYRDAKAKLF+RMVD
Sbjct: 421 GTEAVVMEVSSDGLARGRCDEVDFDIAVFTNLT-RDHLDFQGSEEEYRDAKAKLFKRMVD 480

Query: 481 PDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQ 540
           PDRHRKV+NIDDPNAPFFIGQGNPDVPVVTFAMENKNA VHPLKYELSLFETQVLVHTPQ
Sbjct: 481 PDRHRKVINIDDPNAPFFIGQGNPDVPVVTFAMENKNADVHPLKYELSLFETQVLVHTPQ 540

Query: 541 GILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGV 600
           GILEISSGLLGKHNIYNILAAV+VGIAVGAPLEDIVRG+EEVDAVPGRFELIDEEQAFGV
Sbjct: 541 GILEISSGLLGKHNIYNILAAVAVGIAVGAPLEDIVRGVEEVDAVPGRFELIDEEQAFGV 600

Query: 601 IVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSD 660
           IVD A TPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSD+TILTSD
Sbjct: 601 IVDHAHTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDVTILTSD 660

Query: 661 NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMG 720
           NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPL NGHRIFLHDIRRVAVRA VAMG
Sbjct: 661 NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLSNGHRIFLHDIRRVAVRAAVAMG 720

Query: 721 EEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSEFPWRCVPE 780
           EEGDVVVVAGKGHETYQIE DK DFFDDREECREALQYVDELHQAGIDTSEFPWR +PE
Sbjct: 721 EEGDVVVVAGKGHETYQIEDDKTDFFDDREECREALQYVDELHQAGIDTSEFPWR-LPE 777

BLAST of Cp4.1LG05g03350 vs. NCBI nr
Match: gi|659073856|ref|XP_008437290.1| (PREDICTED: uncharacterized protein LOC103482756 [Cucumis melo])

HSP 1 Score: 1401.7 bits (3627), Expect = 0.0e+00
Identity = 711/779 (91.27%), Postives = 742/779 (95.25%), Query Frame = 1

Query: 1   MASTFTFLPFPLILSPRPSIPCVRPQFRRQYFPTIRILRPSVPSAIGPDGKFYPNPADDD 60
           MASTFTFLPFPLILSPRP+ P +RPQF RQY P IRILRPSVPSAIGPDGKFYP+PA+DD
Sbjct: 1   MASTFTFLPFPLILSPRPNFPSIRPQFHRQYLPAIRILRPSVPSAIGPDGKFYPDPAEDD 60

Query: 61  PPEAPEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAEYLNSE 120
           PPEAPED+GHGVSKFQQIYRQAARA++IQEEDFKKHQSTYLSAIADVEDAP+NAEYLNSE
Sbjct: 61  PPEAPEDSGHGVSKFQQIYRQAARARRIQEEDFKKHQSTYLSAIADVEDAPENAEYLNSE 120

Query: 121 SSGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDKDILEGIDELETEEVVDLEEIN 180
           SSGDDLFGEIDKAIA+KRKEFVKQGLLKPNPKKEK  +KD LEGIDELETEEV DLEEIN
Sbjct: 121 SSGDDLFGEIDKAIALKRKEFVKQGLLKPNPKKEKVGEKDELEGIDELETEEVADLEEIN 180

Query: 181 ELQGLTEISEDENEDKDGDEEGPGNLDDDARELHGSDELSSFNSFDVDFDSYGKVKTRIV 240
           EL+GLT ISEDE  ++D +E+GP NLDD+   L   DELSSFNSFDVDFD+YGKVK RIV
Sbjct: 181 ELRGLTVISEDEEVEEDENEDGPRNLDDNVSALGHEDELSSFNSFDVDFDNYGKVKARIV 240

Query: 241 EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLYL 300
           EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSR+V AG LFVCCVGRETDGHLYL
Sbjct: 241 EPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRVVTAGDLFVCCVGRETDGHLYL 300

Query: 301 TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVTG 360
           TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFY NPSKNMAVIG+TG
Sbjct: 301 TEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYRNPSKNMAVIGITG 360

Query: 361 TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLHN 420
           TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLE+PSTTPDAILVQNLMAKMLHN
Sbjct: 361 TDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLESPSTTPDAILVQNLMAKMLHN 420

Query: 421 GTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMVD 480
           GTEAVVMEVS+  LARGRCDEVDFDIAVFTNLT + +LDFQ SEEEYRDAKAKLF+RMVD
Sbjct: 421 GTEAVVMEVSSDGLARGRCDEVDFDIAVFTNLTSD-HLDFQGSEEEYRDAKAKLFKRMVD 480

Query: 481 PDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQ 540
           PDRHRKV+NIDDPNAPFFIGQGN DVPVVTFAMENKNA VHPLKYELSLFETQVLVHTPQ
Sbjct: 481 PDRHRKVINIDDPNAPFFIGQGNLDVPVVTFAMENKNADVHPLKYELSLFETQVLVHTPQ 540

Query: 541 GILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGV 600
           GILEISSGLLGKHNIYNILAAV+VGIAVGAPLEDIVRG+EEVDAVPGRFELIDEEQAFGV
Sbjct: 541 GILEISSGLLGKHNIYNILAAVAVGIAVGAPLEDIVRGVEEVDAVPGRFELIDEEQAFGV 600

Query: 601 IVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSD 660
           IVD A TPDGLSRLLDSVRELGPRRIITVFGCCGEH+RGKRPMMTKIATDKSD+TILTSD
Sbjct: 601 IVDHAHTPDGLSRLLDSVRELGPRRIITVFGCCGEHERGKRPMMTKIATDKSDVTILTSD 660

Query: 661 NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMG 720
           NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPL NGHRIFLHDIRRVAVRA VAMG
Sbjct: 661 NPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLSNGHRIFLHDIRRVAVRAAVAMG 720

Query: 721 EEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSEFPWRCVPE 780
           EEGDVVVVAGKGHETYQIEGDK +FFDDREECREALQYVDELHQAGIDTSEFPWR +PE
Sbjct: 721 EEGDVVVVAGKGHETYQIEGDKTEFFDDREECREALQYVDELHQAGIDTSEFPWR-LPE 777

BLAST of Cp4.1LG05g03350 vs. NCBI nr
Match: gi|720080119|ref|XP_010241881.1| (PREDICTED: uncharacterized protein LOC104586371 [Nelumbo nucifera])

HSP 1 Score: 1203.7 bits (3113), Expect = 0.0e+00
Identity = 618/775 (79.74%), Postives = 687/775 (88.65%), Query Frame = 1

Query: 15  SPRPSIPCVR-----PQFRRQYFPTIRILR--PSVPSAIGPDGKFYPNPADDDPPEAPED 74
           SP P  P        P  + +  P++R+LR  P +P+AIGPDGKFYP PADDDPPEAPED
Sbjct: 12  SPSPQFPSFSYSIKAPFLQFKSLPSLRLLRQKPRIPTAIGPDGKFYPTPADDDPPEAPED 71

Query: 75  AGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAEYLNSESSGDDLF 134
           +GHGVSKFQQI+RQAARA+K+QEE FKK QS ++SA+A+ EDAPD+ E  NSE+SGDDLF
Sbjct: 72  SGHGVSKFQQIHRQAARARKLQEEQFKKDQSIFVSALAEEEDAPDDPESTNSENSGDDLF 131

Query: 135 GEIDKAIAMKRKEFVKQGLLKPNPKK---EKAIDKDILEGIDELETEEVVDLEEINELQG 194
           GEID+AIA+KRKEFVKQGLLKPNPKK   +KA+D +  EGIDELE EEV+DL+EI +LQG
Sbjct: 132 GEIDRAIALKRKEFVKQGLLKPNPKKGKGQKAVDVN-GEGIDELEPEEVMDLDEIQKLQG 191

Query: 195 LTEISEDENEDKDGDEEGPGNLDDDARELHGSDELSSFNSFDVDFDSYGKVKTRIVEPKF 254
           +T ISE      D DEE P      A ++  S   +S +SFD+DFDSYGK K RIVEPKF
Sbjct: 192 ITVISE------DSDEENPVKF---AEDVSNSSSRTSSSSFDLDFDSYGKSKPRIVEPKF 251

Query: 255 KMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLYLTEAD 314
           +MTLAELLDESKVVPVSV+GNLE+EITGIQHDSR V+AG LFVCCVG +TDGHLYL+EAD
Sbjct: 252 RMTLAELLDESKVVPVSVYGNLEVEITGIQHDSREVSAGDLFVCCVGMKTDGHLYLSEAD 311

Query: 315 KRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVTGTDGK 374
           KRGAVAVVASKEIDI+ETLGCKALV+VEDTNSVLPALAASFY NPSK+M+VIG+TGT+GK
Sbjct: 312 KRGAVAVVASKEIDIDETLGCKALVIVEDTNSVLPALAASFYRNPSKSMSVIGITGTNGK 371

Query: 375 TSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLHNGTEA 434
           T+T+YLIKGMYEAM LRTG+L TVAYY+HGDNKLE+P+TTPDA+LVQ LMAKM HNGTEA
Sbjct: 372 TTTAYLIKGMYEAMALRTGMLSTVAYYVHGDNKLESPNTTPDAVLVQKLMAKMFHNGTEA 431

Query: 435 VVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMVDPDRH 494
           VVME S+  LA GRCDEVDFDIAVFTNLT   ++DF  SEEEYRDAKAKLF RMVDP+RH
Sbjct: 432 VVMEASSHGLALGRCDEVDFDIAVFTNLT-RDHMDFHGSEEEYRDAKAKLFARMVDPERH 491

Query: 495 RKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTPQGILE 554
           RK+VNIDDP+APFFI QGNPDVPVVTFAMENK+A VHPLK+ELSLFETQVLV+TPQGILE
Sbjct: 492 RKIVNIDDPHAPFFIAQGNPDVPVVTFAMENKSADVHPLKFELSLFETQVLVNTPQGILE 551

Query: 555 ISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFGVIVDR 614
           ISSGLLG+HNIYNILAAV+VGIAVGAPLEDIVRGIEEVDAVPGR ELIDEEQAFGVIVD 
Sbjct: 552 ISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVRGIEEVDAVPGRCELIDEEQAFGVIVDY 611

Query: 615 AQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTSDNPRN 674
           A TPD LSRLLDSVRELGPRRIITVFGC GE DRGKRPMMTKIATDKSD+ ILTSDNP+N
Sbjct: 612 AHTPDALSRLLDSVRELGPRRIITVFGCVGEGDRGKRPMMTKIATDKSDVVILTSDNPKN 671

Query: 675 EDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAMGEEGD 734
           EDPL+ILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHR+FLHDIRRVAVRA VAMGEEGD
Sbjct: 672 EDPLNILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRLFLHDIRRVAVRAAVAMGEEGD 731

Query: 735 VVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSEFPWRCVPE 780
           +VV+AGKGHETYQIEGDKK+FFDDREECREALQYVDELHQAGIDTSEFPWR +PE
Sbjct: 732 MVVIAGKGHETYQIEGDKKEFFDDREECREALQYVDELHQAGIDTSEFPWR-LPE 774

BLAST of Cp4.1LG05g03350 vs. NCBI nr
Match: gi|595861263|ref|XP_007211301.1| (hypothetical protein PRUPE_ppa001774mg [Prunus persica])

HSP 1 Score: 1186.8 bits (3069), Expect = 0.0e+00
Identity = 610/780 (78.21%), Postives = 682/780 (87.44%), Query Frame = 1

Query: 5   FTFLPFPLILSPRPSIPCVRPQFR--RQYFPTI--RILRPSVPSAIGPDGKFYPNPADDD 64
           FTFL  P  LSP+P+   ++P F   R   P++  R++RP+   AIGPDGK+YP+PADDD
Sbjct: 3   FTFLSIPHFLSPQPNFLSLKPNFTSLRPSIPSLSARLIRPT--HAIGPDGKYYPDPADDD 62

Query: 65  PPEAPEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAEYLNSE 124
           PPEAPED+GHGVSKFQQI RQA+R +K+QEEDFKKHQ T+L+AIADVED P+N+  + +E
Sbjct: 63  PPEAPEDSGHGVSKFQQIQRQASRHRKLQEEDFKKHQDTFLNAIADVEDPPENSSSVTNE 122

Query: 125 SSGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDKDILEGIDELETEEVVDLEEIN 184
           +SGDDLFG+ID AIA+KRKEFVKQGLLKPNPKKE       +  +DEL+ EEVVDLEEI+
Sbjct: 123 NSGDDLFGDIDHAIALKRKEFVKQGLLKPNPKKE-------IVAVDELDPEEVVDLEEID 182

Query: 185 ELQGLTEISEDENEDKDGDEEGPGNLDDDARELHGSDELSSFNS-FDVDFDSYGKVKTRI 244
           ELQGL  +SED       DE+GP   D    +L G D     NS FD+DFDSYGK + RI
Sbjct: 183 ELQGLRVVSEDL------DEDGPEKFDSKVSDLDGKDGNLRLNSEFDLDFDSYGKTRARI 242

Query: 245 VEPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETDGHLY 304
           VEPKFKM+LAELLDESKVVPVSV+G+LE+EITGIQHDSR+VN+G LFVCCVG +TDGHLY
Sbjct: 243 VEPKFKMSLAELLDESKVVPVSVYGDLEVEITGIQHDSRVVNSGDLFVCCVGSKTDGHLY 302

Query: 305 LTEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAVIGVT 364
           L+EA KRGAVAVVASKEI +EE LGCKALV+VEDTN+ LPALAASFY  PSKNMAVIG+T
Sbjct: 303 LSEAIKRGAVAVVASKEIYMEENLGCKALVIVEDTNADLPALAASFYRYPSKNMAVIGIT 362

Query: 365 GTDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMAKMLH 424
           GT+GKT+T+YLIKG+YEAMGLRTG+L TVAYY+HGDNKLE+P+TTPDA+LVQNLMAKMLH
Sbjct: 363 GTNGKTTTAYLIKGLYEAMGLRTGMLSTVAYYVHGDNKLESPNTTPDAVLVQNLMAKMLH 422

Query: 425 NGTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLFRRMV 484
           NG EAVVME S+  LA  RCDEVDFDIAVFTNLT + +LDF ++EEEYRDAKAKLF RMV
Sbjct: 423 NGAEAVVMEASSHGLALARCDEVDFDIAVFTNLTRD-HLDFHKTEEEYRDAKAKLFSRMV 482

Query: 485 DPDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVLVHTP 544
           DP+RHRKVVNIDDPNA FFI QGNPD+PVVTFAMENKNA VHPLK+ELSLFETQVLV TP
Sbjct: 483 DPERHRKVVNIDDPNATFFIAQGNPDIPVVTFAMENKNADVHPLKFELSLFETQVLVSTP 542

Query: 545 QGILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEEQAFG 604
           QGILEISSGLLG+HNIYNILAAV+VGIAVGAPLEDIVRGIEEVDAVPGR ELIDEEQAFG
Sbjct: 543 QGILEISSGLLGRHNIYNILAAVAVGIAVGAPLEDIVRGIEEVDAVPGRCELIDEEQAFG 602

Query: 605 VIVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDITILTS 664
           VIVD A TPD LSRLLDSVRELG RRII+V GC GE DRGKRPMMTKIATDKSD+TILTS
Sbjct: 603 VIVDYAHTPDALSRLLDSVRELGARRIISVIGCPGESDRGKRPMMTKIATDKSDVTILTS 662

Query: 665 DNPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRAGVAM 724
           DNP+NEDPLDILDDMLAG+GWTMQDYLKHGENDYYPPLPNGHR+FLHDIRRVAVR  VAM
Sbjct: 663 DNPKNEDPLDILDDMLAGVGWTMQDYLKHGENDYYPPLPNGHRLFLHDIRRVAVRCAVAM 722

Query: 725 GEEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSEFPWRCVPE 780
           GEEGD+VVVAGKGHE YQIEGDKK+FFDDREECREALQYVDELHQAGIDTSEFPWR +PE
Sbjct: 723 GEEGDMVVVAGKGHEAYQIEGDKKEFFDDREECREALQYVDELHQAGIDTSEFPWR-LPE 765

BLAST of Cp4.1LG05g03350 vs. NCBI nr
Match: gi|1009156137|ref|XP_015896086.1| (PREDICTED: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase [Ziziphus jujuba])

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 612/784 (78.06%), Postives = 687/784 (87.63%), Query Frame = 1

Query: 5   FTFLPFPLILSPR-------PSIPCVRPQFRRQYFPTIRILRPSVPSAIGPDGKFYPNPA 64
           F  L  P IL+P+       P IP ++P F      ++R      PSAIGPDGKFYPNP+
Sbjct: 3   FACLSIPHILTPQHHFLTLNPRIPLLKPIFPLAPL-SLRTRPVGCPSAIGPDGKFYPNPS 62

Query: 65  DDDPPEAPEDAGHGVSKFQQIYRQAARAKKIQEEDFKKHQSTYLSAIADVEDAPDNAEYL 124
           DDDPPEAPED+GHGVSKFQQI RQA+RA+KIQEE+FKKHQST+LSAIADVED  +N   +
Sbjct: 63  DDDPPEAPEDSGHGVSKFQQIQRQASRARKIQEEEFKKHQSTFLSAIADVEDTTENPGSV 122

Query: 125 NSES-SGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKEKAIDKDILEGIDELETEEVVDL 184
           +S++ SGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKE     +++E ++ELE EEVVDL
Sbjct: 123 SSDNNSGDDLFGEIDKAIAMKRKEFVKQGLLKPNPKKE-----EVVENVEELEPEEVVDL 182

Query: 185 EEINELQGLTEISEDENEDKDGDEEGPGNLDDDARELHGSDELSSFNS-FDVDFDSYGKV 244
           EEI+ELQGLT  SED   D  G E+     DD+   +  +D +SS +S FD+DFDSYGK 
Sbjct: 183 EEIDELQGLTVTSEDS--DSHGSEKFD---DDEGNGVSKTDRISSVSSSFDLDFDSYGKA 242

Query: 245 KTRIVEPKFKMTLAELLDESKVVPVSVFGNLEIEITGIQHDSRMVNAGYLFVCCVGRETD 304
           K RI+EPKFK++LAELLDESK+VPVSV+G+LE+EITGIQHDSR+V+ G LFVCCVG + D
Sbjct: 243 KPRILEPKFKISLAELLDESKIVPVSVYGDLEVEITGIQHDSRLVSLGDLFVCCVGLKND 302

Query: 305 GHLYLTEADKRGAVAVVASKEIDIEETLGCKALVMVEDTNSVLPALAASFYMNPSKNMAV 364
           GHLYLTEADKRGAVAVVASKEIDIE+TLGCKALV+VEDTN+VLP LAA+FY  PSKNMAV
Sbjct: 303 GHLYLTEADKRGAVAVVASKEIDIEDTLGCKALVIVEDTNAVLPVLAAAFYRYPSKNMAV 362

Query: 365 IGVTGTDGKTSTSYLIKGMYEAMGLRTGLLGTVAYYLHGDNKLEAPSTTPDAILVQNLMA 424
           IG+TGT+GKT+TSYLIKGMYEAMGLRTG+L TV+YY+HGDNKLE+P+TTPDA+LVQN+MA
Sbjct: 363 IGITGTNGKTTTSYLIKGMYEAMGLRTGMLSTVSYYVHGDNKLESPNTTPDAVLVQNMMA 422

Query: 425 KMLHNGTEAVVMEVSTAELARGRCDEVDFDIAVFTNLTPESNLDFQESEEEYRDAKAKLF 484
           KMLHNG EAVVME S+  LA GRC+EVDFDIAVFTNLT + ++DF ESEE+Y++AKAKLF
Sbjct: 423 KMLHNGAEAVVMEASSHGLALGRCNEVDFDIAVFTNLTRD-HMDFHESEEDYQNAKAKLF 482

Query: 485 RRMVDPDRHRKVVNIDDPNAPFFIGQGNPDVPVVTFAMENKNAAVHPLKYELSLFETQVL 544
            RMVDP+RHRKVVNIDDPNAPFFI QGNPDVPVVTFAMENKNA VHPLK+ELSLFETQVL
Sbjct: 483 SRMVDPERHRKVVNIDDPNAPFFISQGNPDVPVVTFAMENKNADVHPLKFELSLFETQVL 542

Query: 545 VHTPQGILEISSGLLGKHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRFELIDEE 604
           V+TP GILEISSGLLG+HNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGR ELIDEE
Sbjct: 543 VNTPHGILEISSGLLGRHNIYNILAAVSVGIAVGAPLEDIVRGIEEVDAVPGRCELIDEE 602

Query: 605 QAFGVIVDRAQTPDGLSRLLDSVRELGPRRIITVFGCCGEHDRGKRPMMTKIATDKSDIT 664
           QAFGVIVD A +PD LSRLLDSVREL PRRIITV GC GE DRGKRPMM KIATDKS++T
Sbjct: 603 QAFGVIVDYAHSPDALSRLLDSVRELRPRRIITVVGCGGERDRGKRPMMAKIATDKSEVT 662

Query: 665 ILTSDNPRNEDPLDILDDMLAGIGWTMQDYLKHGENDYYPPLPNGHRIFLHDIRRVAVRA 724
           ILTSDNPRNEDPLDILDDMLAG+GWTMQDYLK+GENDYYPPLPNGHR+FLHDIRRVAVR 
Sbjct: 663 ILTSDNPRNEDPLDILDDMLAGVGWTMQDYLKYGENDYYPPLPNGHRLFLHDIRRVAVRC 722

Query: 725 GVAMGEEGDVVVVAGKGHETYQIEGDKKDFFDDREECREALQYVDELHQAGIDTSEFPWR 780
            VAMGEEGD+VVVAGKGHETYQIEGDK +FFDDREECREALQYVDELHQAGIDTSEFPWR
Sbjct: 723 AVAMGEEGDMVVVAGKGHETYQIEGDKTEFFDDREECREALQYVDELHQAGIDTSEFPWR 773