Cp4.1LG05g02550 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG05g02550
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCytochrome P450 family protein
LocationCp4.1LG05 : 1508449 .. 1510333 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGTTAATTAAGTTAGGTTCCTGTTGGAGAATTATTTACATAATTAAAATGTTATAAATTTAAGTTGAAAATAGTAACATTAAAAATTGTTAATTACATAATTAAATATTACAGAATAATTATTATAATATAATTAGTTAATTAAGTAATTATTGGAGTAGAGTTGGAACTTGAGAATTGAGACGGTGATATGGAAATATAATCGTCTACTCCTCCTTCACTCTCTATTCCTGATTCCATCATGGAACCCACTTTCAATTTCCTTCAATCTCTCAATTCCTTGTTTCCATGGCATTCCGGCCACAATTACCCCTTGATTTTCACTCTCCTTGCCGGTTTTCTCGTTCTTTTGTCTTTCAGATTCCGGCGCCGGCGCTTGCAGCTTCCACCCGGACCCCCCGGCGTTCCTCTGCTTGGAAATCTACCGTTTCTAGATCCAGAGCTTCACACTTACTTCACCGAATTGGCCCGAAAATACGGTCCAATTGTAAAGCTCCAACTCGGTTCAAAGATCGGTATAATCATCAACTCACCCTCTGTCGCACGTGAGGTGTTGAAGGACCACGATATCACGTTTGCCAACCACGACGTTCCTCGGGCAGCTAGAGCCGCCACATACGGCGGTTACGACATAGTTTGGAGTCCATACGGACCCGAGTGGCGGATGTTGAGAAAAGTTTGTACGATGAAGATGCTCAGTAACGCTAGTTTGGACTCTGTCTACGAGCTCCGCCGTAGAGAGGTCAGAAACACCGTTGCTCACCTATACCGGCGAGCCGGGTCGGCGGTGAACATAGGAGAACAGGGTTTTTTGACGGTTTTCAACATCGTGACAAGCATGTTGTGGGGTGGGTCGGTGGATGGAGACGAAAGGGACGGGATTGCGGCTGAGTTCAGAGAGCTAATTTCGGACATAACTTTGCTGTTGGGTAAGCCTAATGTGTCGGACTTTTTCCCGAGGTTGGCTCGTTTCGATCTCCAAGGCATCGAGAAACAAATGCTTAAGCTCGCACTAAAATTGGACAACATCTTCGAAAAGATGATCAATCAACGACTGAGAATGGCCGATGGGGACGAGAAGCAGAACAAAAACGATTTCTTGCAATTCTTACTTCAGATCAAAGATGAAGGCGATTCCAAAACGCCTCTCACCATGGTTCAAATCAAAGGCGTGCTCATGGTAATTATTTTCTTGATTTTGAGTGGAAAAGAACAATTTCAAAGAAGAATTTATTTTTAATGAAATTGAATTTGCGGACAGGACATGGTGGTAGGTGGAACAGACACATCCTCAAACACCATTGAGTTCGCAATGGCAGAGATGATGATGAACCCAAATGTTCTAAAGAAAGTGCAAGAAGAATTAGCCGTCGTGATCGGAGACGAGAACATCGTAGAAGAGTCCCATATTGAGAGTCTGCCATATCTGAAAGCCGTAGTGAAAGAAACGTTGCGTCTGCATCCAGTGTTGCCGCTGCTAGTGCCGCACTGTCCGAGCGAGACCACCATGATATCTAATTACACAATCCCAAAGGGGTCTCGAGTTTTCATCAACGTCTGGGCCATTCAGCGAGACCCCAATCAATGGAGAAACCCATTGGAGTTTGAGCCGGAGAGATTCTTGAATGATAAGATGGATTTCAACGGGAGTGACTTTCGATTCATTCCATTTGGGTCAGGGAGAAGAAACTGCGCAGGGACAGGGTTGGCGGAAAGAATGATTATGTATTTGCTTGCGACGTTTCTGCACTCGTTTGATTGGAAATTGAAAGAGGGTGAGGAATTGAAGACCGTCGAGAAATTTGGGATTGTTTTGAAGATGAAGACTCCTCTGGTTGCCATCTGCACTCCCAGCCTCTCTCACCCCACTCTGTATCAGTAA

mRNA sequence

ATGATATTCCGGCGCCGGCGCTTGCAGCTTCCACCCGGACCCCCCGGCGTTCCTCTGCTTGGAAATCTACCGTTTCTAGATCCAGAGCTTCACACTTACTTCACCGAATTGGCCCGAAAATACGGTCCAATTGTAAAGCTCCAACTCGGTTCAAAGATCGGTATAATCATCAACTCACCCTCTGTCGCACGTGAGGTGTTGAAGGACCACGATATCACGTTTGCCAACCACGACGTTCCTCGGGCAGCTAGAGCCGCCACATACGGCGGTTACGACATAGTTTGGAGTCCATACGGACCCGAGTGGCGGATGTTGAGAAAAGTTTGTACGATGAAGATGCTCAGTAACGCTAGTTTGGACTCTGTCTACGAGCTCCGCCGTAGAGAGGTCAGAAACACCGTTGCTCACCTATACCGGCGAGCCGGGTCGGCGGTGAACATAGGAGAACAGGGTTTTTTGACGGTTTTCAACATCGTGACAAGCATGTTGTGGGGTGGGTCGGTGGATGGAGACGAAAGGGACGGGATTGCGGCTGAGTTCAGAGAGCTAATTTCGGACATAACTTTGCTGTTGGGTAAGCCTAATGTGTCGGACTTTTTCCCGAGGTTGGCTCGTTTCGATCTCCAAGGCATCGAGAAACAAATGCTTAAGCTCGCACTAAAATTGGACAACATCTTCGAAAAGATGATCAATCAACGACTGAGAATGGCCGATGGGGACGAGAAGCAGAACAAAAACGATTTCTTGCAATTCTTACTTCAGATCAAAGATGAAGGCGATTCCAAAACGCCTCTCACCATGGTTCAAATCAAAGGCGTGCTCATGACCGTCGAGAAATTTGGGATTGTTTTGAAGATGAAGACTCCTCTGGTTGCCATCTGCACTCCCAGCCTCTCTCACCCCACTCTGTATCAGTAA

Coding sequence (CDS)

ATGATATTCCGGCGCCGGCGCTTGCAGCTTCCACCCGGACCCCCCGGCGTTCCTCTGCTTGGAAATCTACCGTTTCTAGATCCAGAGCTTCACACTTACTTCACCGAATTGGCCCGAAAATACGGTCCAATTGTAAAGCTCCAACTCGGTTCAAAGATCGGTATAATCATCAACTCACCCTCTGTCGCACGTGAGGTGTTGAAGGACCACGATATCACGTTTGCCAACCACGACGTTCCTCGGGCAGCTAGAGCCGCCACATACGGCGGTTACGACATAGTTTGGAGTCCATACGGACCCGAGTGGCGGATGTTGAGAAAAGTTTGTACGATGAAGATGCTCAGTAACGCTAGTTTGGACTCTGTCTACGAGCTCCGCCGTAGAGAGGTCAGAAACACCGTTGCTCACCTATACCGGCGAGCCGGGTCGGCGGTGAACATAGGAGAACAGGGTTTTTTGACGGTTTTCAACATCGTGACAAGCATGTTGTGGGGTGGGTCGGTGGATGGAGACGAAAGGGACGGGATTGCGGCTGAGTTCAGAGAGCTAATTTCGGACATAACTTTGCTGTTGGGTAAGCCTAATGTGTCGGACTTTTTCCCGAGGTTGGCTCGTTTCGATCTCCAAGGCATCGAGAAACAAATGCTTAAGCTCGCACTAAAATTGGACAACATCTTCGAAAAGATGATCAATCAACGACTGAGAATGGCCGATGGGGACGAGAAGCAGAACAAAAACGATTTCTTGCAATTCTTACTTCAGATCAAAGATGAAGGCGATTCCAAAACGCCTCTCACCATGGTTCAAATCAAAGGCGTGCTCATGACCGTCGAGAAATTTGGGATTGTTTTGAAGATGAAGACTCCTCTGGTTGCCATCTGCACTCCCAGCCTCTCTCACCCCACTCTGTATCAGTAA

Protein sequence

MIFRRRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYELRRREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRELISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDEKQNKNDFLQFLLQIKDEGDSKTPLTMVQIKGVLMTVEKFGIVLKMKTPLVAICTPSLSHPTLYQ
BLAST of Cp4.1LG05g02550 vs. Swiss-Prot
Match: C76BA_SWEMU (Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.4e-39
Identity = 90/269 (33.46%), Postives = 146/269 (54.28%), Query Frame = 1

Query: 3   FRRRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSV 62
           F R+   LPPGP  +PL+GNL  L  + H    +LA+K+GPI+ LQLG    I++ S  +
Sbjct: 25  FSRKSKNLPPGPSPLPLIGNLHLLGDQPHKSLAKLAKKHGPIMGLQLGQVTTIVVTSSGM 84

Query: 63  AREVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSV 122
           A+EVL+  D+ F++  +P A  A     Y ++W P    WR LRK     M S   LD+ 
Sbjct: 85  AKEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVASRWRGLRKALNSNMFSGNRLDAN 144

Query: 123 YELRRREVRNTVAHLYR--RAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEF 182
             LR R+V+  +A+  +  + G A+++G   F T  N++++ ++   +     D  A EF
Sbjct: 145 QHLRSRKVQELIAYCRKSSQTGDAIDVGRAAFRTSLNLLSNTMFSKDLTDPYSDS-AKEF 204

Query: 183 RELISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGD 242
           ++L+ ++ +  GKPN+ D+FP L + D QGI K+M     K+  +F  +I++RL+     
Sbjct: 205 KDLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKILELFGGLIDERLQQKKA- 264

Query: 243 EKQNKNDFLQFLLQIKDEGDSKTPLTMVQ 270
            K   +D L  LL   +E   +   T +Q
Sbjct: 265 -KGVNDDVLDVLLTTSEESPEEIDRTHIQ 290

BLAST of Cp4.1LG05g02550 vs. Swiss-Prot
Match: C76B6_CATRO (Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 7.1e-39
Identity = 90/267 (33.71%), Postives = 147/267 (55.06%), Query Frame = 1

Query: 5   RRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAR 64
           RR   LPPGP  +P +G+L  L  + H    +L++K+GPI+ L+LG    I+I+S ++A+
Sbjct: 25  RRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKKHGPIMSLKLGQITTIVISSSTMAK 84

Query: 65  EVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYE 124
           EVL+  D+ F++  VP A  A     + +VW P    WR LRKV    + S   LD+   
Sbjct: 85  EVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVASRWRSLRKVLNSNIFSGNRLDANQH 144

Query: 125 LRRREVRNTVAHLYR--RAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRE 184
           LR R+V+  +A+  +  ++G AV++G   F T  N+++++++   +     D  A EF++
Sbjct: 145 LRTRKVQELIAYCRKNSQSGEAVDVGRAAFRTSLNLLSNLIFSKDLTDPYSDS-AKEFKD 204

Query: 185 LISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDEK 244
           L+ +I +  GKPN+ DFFP L + D QGI  +M     ++  +F  ++N+RL       K
Sbjct: 205 LVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEVLKLFGGLVNERLEQR--RSK 264

Query: 245 QNKNDFLQFLLQIKDEGDSKTPLTMVQ 270
             KND L  LL    E   +   T ++
Sbjct: 265 GEKNDVLDVLLTTSQESPEEIDRTHIE 288

BLAST of Cp4.1LG05g02550 vs. Swiss-Prot
Match: C75A2_SOLME (Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.1e-37
Identity = 90/302 (29.80%), Postives = 163/302 (53.97%), Query Frame = 1

Query: 6   RRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVARE 65
           RR +LPPGP G P++G LP L    H    ++A+KYGPI+ L++G+   ++ ++P+ A+ 
Sbjct: 32  RRRRLPPGPEGWPVIGALPLLGGMPHVALAKMAKKYGPIMYLKVGTCGMVVASTPNAAKA 91

Query: 66  VLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYEL 125
            LK  DI F+N      A    Y   D+V++PYGP W++LRK+  + ML   +L++   +
Sbjct: 92  FLKTLDINFSNRPPNAGATHMAYNAQDMVFAPYGPRWKLLRKLSNLHMLGGKALENWANV 151

Query: 126 RRREVRNTVAHLY--RRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFREL 185
           R  E+ + +  ++     G  + + +     + N++  ++    V   E+     EF+ +
Sbjct: 152 RANELGHMLKSMFDASHVGERIVVADMLTFAMANMIGQVMLSKRV-FVEKGKEVNEFKNM 211

Query: 186 ISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDEKQ 245
           + ++  + G  N+ DF P++A  DLQGIEK M KL  K D++  KM  +    A  +E++
Sbjct: 212 VVELMTVAGYFNIGDFIPQIAWMDLQGIEKGMKKLHKKFDDLLTKMFEE--HEATSNERK 271

Query: 246 NKNDFLQFLLQIKDEGDSKTPLTMVQIKGVLMTVEKFGIVLKMKTPLVAICTPSLSHPTL 305
            K DFL F++  +D  + +  L++  IK +L+ +   G          A+ T  + +PT+
Sbjct: 272 GKPDFLDFIMANRDNSEGER-LSITNIKALLLNLFTAGTDTSSSVIEWAL-TEMMKNPTI 328

BLAST of Cp4.1LG05g02550 vs. Swiss-Prot
Match: CYPH1_SALMI (Ferruginol synthase OS=Salvia miltiorrhiza GN=CYP76AH1 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.1e-37
Identity = 88/264 (33.33%), Postives = 150/264 (56.82%), Query Frame = 1

Query: 3   FRRRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSV 62
           FRRRR  LPPGP   P++GN+  L    H  F +L+++YGP++ + LGS   +I++SP +
Sbjct: 22  FRRRR-NLPPGPFPYPIVGNMLQLGANPHQVFAKLSKRYGPLMSIHLGSLYTVIVSSPEM 81

Query: 63  AREVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSV 122
           A+E+L  H   F+   + +A  A  +    + + P   EWR +RK+C  +M SN S+++ 
Sbjct: 82  AKEILHRHGQVFSGRTIAQAVHACDHDKISMGFLPVASEWRDMRKICKEQMFSNQSMEAS 141

Query: 123 YELRRREVRNTVAHLYR--RAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAA-E 182
             LRR++++  + H+ +   +G AV+I E  F+T  N++++ L+  S    E D  A  E
Sbjct: 142 QGLRRQKLQQLLDHVQKCSDSGRAVDIREAAFITTLNLMSATLF--SSQATEFDSKATME 201

Query: 183 FRELISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADG 242
           F+E+I  +  ++G PN +D+FP L  FD QG++++      KL    E  +N+RL     
Sbjct: 202 FKEIIEGVATIVGVPNFADYFPILRPFDPQGVKRRADVFFGKLLAKIEGYLNERLESKRA 261

Query: 243 D-EKQNKNDFLQFLLQIKDEGDSK 263
           +     K+DFL+ ++ I    + K
Sbjct: 262 NPNAPKKDDFLEIVVDIIQANEFK 282

BLAST of Cp4.1LG05g02550 vs. Swiss-Prot
Match: C7BL2_LACSA (Costunolide synthase OS=Lactuca sativa GN=CYP71BL2 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 7.3e-36
Identity = 85/268 (31.72%), Postives = 145/268 (54.10%), Query Frame = 1

Query: 10  LPPGPPGVPLLGNLPFL-DPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREVLK 69
           LPPGPP +P++GN+  L  P  H     LA+KYGPI+ LQLG    +++++P +ARE++K
Sbjct: 30  LPPGPPKLPIIGNIHQLKSPTPHRVLRNLAKKYGPIMHLQLGQVSTVVVSTPRLAREIMK 89

Query: 70  DHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYELRRR 129
            +DI+FA+      ++   Y   DI W+PYG  WR ++K+CT+++LS   + S   +R  
Sbjct: 90  TNDISFADRPTTTTSQIFFYKAQDIGWAPYGEYWRQMKKICTLELLSAKKVRSFSSIREE 149

Query: 130 EVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRELISDIT 189
           E+R     L  +AG+ VN  E     V N++     G S          A   E++ D+ 
Sbjct: 150 ELRRISKVLESKAGTPVNFTEMTVEMVNNVICKATLGDSCKDQ------ATLIEVLYDVL 209

Query: 190 LLLGKPNVSDFFPRLARFD-LQGIEKQMLKLALKLDNIFEKMINQRLRMADGDEKQNKND 249
             L   N++ ++P L   + + G + + LK+  +LD+I E ++ +    + G  K ++ D
Sbjct: 210 KTLSAFNLASYYPGLQFLNVILGKKAKWLKMQKQLDDILEDVLKE--HRSKGRNKSDQED 269

Query: 250 FLQFLLQIKDEGDSKTPLTMVQIKGVLM 276
            +  LL++KD G     +T   +K V++
Sbjct: 270 LVDVLLRVKDTGGLDFTVTDEHVKAVVL 289

BLAST of Cp4.1LG05g02550 vs. TrEMBL
Match: A0A0A0KKS3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159620 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 5.7e-128
Identity = 227/274 (82.85%), Postives = 249/274 (90.88%), Query Frame = 1

Query: 3   FRRRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSV 62
           F RRR+ LPPGPPGVPLLGNLPFLDPELH+YFTEL RKYGPIV+LQLG KIGII+NS S+
Sbjct: 38  FSRRRIHLPPGPPGVPLLGNLPFLDPELHSYFTELGRKYGPIVRLQLGGKIGIIVNSSSL 97

Query: 63  AREVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSV 122
           AREVLKDHDITFANHDV  A RAATYGG DI W+PYGPEWRMLRKVCT+KMLSNASLDSV
Sbjct: 98  AREVLKDHDITFANHDVHEAGRAATYGGSDISWAPYGPEWRMLRKVCTIKMLSNASLDSV 157

Query: 123 YELRRREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDE-RDGIAAEFR 182
           YELRRREVRNTVAHLYRRAGSAVN+GEQGFLT+FN+VTSMLWGGSV+ D+ RD IAAEFR
Sbjct: 158 YELRRREVRNTVAHLYRRAGSAVNVGEQGFLTIFNVVTSMLWGGSVESDQSRDNIAAEFR 217

Query: 183 ELISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDE 242
           E IS+IT LLG+PNVSDFFP LARFDLQGIEKQM+K  LKLD IFEKMI++R+RM D +E
Sbjct: 218 ETISEITKLLGQPNVSDFFPSLARFDLQGIEKQMVKHVLKLDTIFEKMIDERVRMKDVNE 277

Query: 243 KQNKNDFLQFLLQIKDEGDSKTPLTMVQIKGVLM 276
              KNDFLQFLL +KDEGDSKTPLTMVQ+KG+LM
Sbjct: 278 SVKKNDFLQFLLTVKDEGDSKTPLTMVQLKGLLM 311

BLAST of Cp4.1LG05g02550 vs. TrEMBL
Match: A0A0A0KR55_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159600 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.1e-118
Identity = 210/273 (76.92%), Postives = 242/273 (88.64%), Query Frame = 1

Query: 5   RRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAR 64
           R R+ LPPGP GVPLLGNLPFL PELHTYF +L +KYGPIVKLQLG KIGIIINSPSV R
Sbjct: 34  RLRVPLPPGPRGVPLLGNLPFLHPELHTYFAQLGQKYGPIVKLQLGRKIGIIINSPSVVR 93

Query: 65  EVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYE 124
           EVLKDHD+TFAN DVP+A RAA+YGG DIVW+PYGP+WRMLRKVC +KMLSNA+LDSVYE
Sbjct: 94  EVLKDHDVTFANRDVPQAGRAASYGGSDIVWTPYGPKWRMLRKVCVVKMLSNATLDSVYE 153

Query: 125 LRRREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRELI 184
           LRR+EVRNTVAHLY RAG+AVN+GEQGFLTVFN+VTSMLWGGSV+G++RDG+AAEFRE +
Sbjct: 154 LRRKEVRNTVAHLYARAGTAVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETV 213

Query: 185 SDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDE--K 244
           S++T LLG PNVSDFFP LARFDLQGIEK+M +LA + D+IFEKMI+QRL++  GDE   
Sbjct: 214 SEMTELLGLPNVSDFFPSLARFDLQGIEKKMRELAPRFDSIFEKMIDQRLKIDGGDEGGS 273

Query: 245 QNKNDFLQFLLQIKDEGDSKTPLTMVQIKGVLM 276
             KNDFLQFLLQ+KDE +SKTPLTM  +K +LM
Sbjct: 274 VKKNDFLQFLLQVKDEEESKTPLTMTHLKALLM 306

BLAST of Cp4.1LG05g02550 vs. TrEMBL
Match: A0A0A0KP59_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159610 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 3.7e-111
Identity = 203/299 (67.89%), Postives = 241/299 (80.60%), Query Frame = 1

Query: 7   RLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREV 66
           RLQLPPGP GVPLLGNLPFLDP LHTYF EL +KYG IVKLQLG K+GII+NSPSVARE+
Sbjct: 39  RLQLPPGPRGVPLLGNLPFLDPNLHTYFMELGQKYGSIVKLQLGGKVGIIVNSPSVAREI 98

Query: 67  LKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYELR 126
           LKDHDITFAN DVP+A R ATYGG+DI W+PYGPEWRMLRKVCT+K+L NASLD VYELR
Sbjct: 99  LKDHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKLLGNASLDMVYELR 158

Query: 127 RREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRELISD 186
           R EVR TVA LY+RA S V IGEQ F TVFN++TSMLWGG+++G+E+  +A  FRE++S 
Sbjct: 159 RSEVRKTVAQLYQRAESTVKIGEQVFFTVFNVITSMLWGGTMEGEEKAAVAVVFREMVSA 218

Query: 187 ITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDE-KQNK 246
           +T L GKPN+SDFFP LA  D+QGIEK+MLKL  KLD IFEK+I++RLRM + +E  +NK
Sbjct: 219 MTELAGKPNISDFFPSLACLDVQGIEKKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNK 278

Query: 247 NDFLQFLLQIKDEGDSKTPLTMVQIKGVLM------------TVEKFGIVLKMKTPLVA 293
           NDFLQFLL++KDE DS+TPLT+VQ+K +LM            T+E F +   MK P VA
Sbjct: 279 NDFLQFLLKVKDESDSQTPLTVVQLKALLMDMVFGGTDTSSNTIE-FAMAEMMKNPKVA 336

BLAST of Cp4.1LG05g02550 vs. TrEMBL
Match: A0A059CH32_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02011 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 4.8e-103
Identity = 182/266 (68.42%), Postives = 221/266 (83.08%), Query Frame = 1

Query: 10  LPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREVLKD 69
           LPPGP G+PL+GNLPFLDPELHTYF  LAR YGP++KLQLG K+G+I+ SP+ AREVLKD
Sbjct: 51  LPPGPAGLPLVGNLPFLDPELHTYFAALARMYGPVLKLQLGKKLGVIVTSPATAREVLKD 110

Query: 70  HDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYELRRRE 129
           +D+ FAN DVP A RAA+YGG DIVW+PYGPEWRMLRKVC +KMLSN +LDSVY+LRRRE
Sbjct: 111 NDVIFANRDVPIAGRAASYGGSDIVWTPYGPEWRMLRKVCVLKMLSNHTLDSVYDLRRRE 170

Query: 130 VRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRELISDITL 189
           VR TV +   RAGS VN+GEQ FLTV N++TSMLWGG+V G+ER+ + A+FR+++SD+T 
Sbjct: 171 VRKTVGYFRVRAGSPVNVGEQMFLTVLNVITSMLWGGTVQGEERESLGADFRQVVSDMTE 230

Query: 190 LLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDEKQNKNDFL 249
           LLGKPNVSDF+P LARFD QGIEK+M  L  + D IFEKMI QRLRM + D + +  DFL
Sbjct: 231 LLGKPNVSDFYPGLARFDFQGIEKRMKGLGKRFDGIFEKMIAQRLRM-ERDGRSHCKDFL 290

Query: 250 QFLLQIKDEGDSKTPLTMVQIKGVLM 276
           QFLL++KDE D+KTPLTM  +K +LM
Sbjct: 291 QFLLELKDEEDAKTPLTMTGLKALLM 315

BLAST of Cp4.1LG05g02550 vs. TrEMBL
Match: U5GNW2_POPTR (Cytochrome P450 family protein OS=Populus trichocarpa GN=POPTR_0001s07850g PE=3 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.4e-102
Identity = 182/274 (66.42%), Postives = 223/274 (81.39%), Query Frame = 1

Query: 4   RRRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVA 63
           ++ R  LPPGP G+PL+GNL  LDP+LHTYF  LAR YGPI+KLQLGSK+GII++SP++A
Sbjct: 28  KKGRPSLPPGPRGLPLIGNLASLDPDLHTYFAGLARTYGPILKLQLGSKLGIIVSSPNLA 87

Query: 64  REVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVY 123
           REVLKDHDITFAN DVP  AR A YGG DI WSPYGPEWRMLRKVC +KMLSN++LDSVY
Sbjct: 88  REVLKDHDITFANRDVPDVARIAAYGGSDIAWSPYGPEWRMLRKVCVLKMLSNSTLDSVY 147

Query: 124 ELRRREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFREL 183
           ELRRREVRN +A++Y + GS +N+GEQ FLT+ N+VTSMLWGG+V G+ER  + AEFR +
Sbjct: 148 ELRRREVRNIIAYIYSKPGSPINVGEQTFLTILNVVTSMLWGGTVQGEERGSLGAEFRRV 207

Query: 184 ISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRM-ADGDEK 243
           ++D+T LLG PN+SDFFP LARFDLQG+ K+M  LA K D IF++MI ++L + A GD  
Sbjct: 208 VADMTELLGAPNISDFFPALARFDLQGLVKKMSGLAPKFDQIFDRMIEKQLSIDALGDRA 267

Query: 244 -QNKNDFLQFLLQIKDEGDSKTPLTMVQIKGVLM 276
             +  DFLQFLL++KDEGD KTPLTM  IK +LM
Sbjct: 268 GASSKDFLQFLLKVKDEGDVKTPLTMTHIKALLM 301

BLAST of Cp4.1LG05g02550 vs. TAIR10
Match: AT4G12320.1 (AT4G12320.1 cytochrome P450, family 706, subfamily A, polypeptide 6)

HSP 1 Score: 330.9 bits (847), Expect = 8.5e-91
Identity = 160/271 (59.04%), Postives = 210/271 (77.49%), Query Frame = 1

Query: 10  LPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREVLKD 69
           LPPGP G+P++GNLPFLDP+LHTYFT+LA  YGPI KL LGSK+ +++N+PS+ARE+LKD
Sbjct: 42  LPPGPRGLPIVGNLPFLDPDLHTYFTKLAESYGPIFKLNLGSKLTVVVNTPSLAREILKD 101

Query: 70  HDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYELRRRE 129
            DI F+NHDVP  ARA TYGG D+VW PYG EWRMLRKVC +K+LS+ +L+S YELRR+E
Sbjct: 102 QDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRKVCVLKLLSHRTLNSFYELRRKE 161

Query: 130 VRNTVAHLYRRA--GSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRELISDI 189
           +R    +LY++    S VN+GEQ FLT+ N+  +MLWGGSV  +E + +  EF+E+IS+I
Sbjct: 162 IRERTRYLYQKGQEESPVNVGEQVFLTMMNLTMNMLWGGSVKAEEMESVGTEFKEVISEI 221

Query: 190 TLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQR--LRMADGDEKQNK 249
           T LLG+PNVSDFFPRLARFDLQG+ K+M   A +LD I ++ I Q   LR  DGD+ + K
Sbjct: 222 TRLLGEPNVSDFFPRLARFDLQGLVKKMHVCARELDAILDRAIEQMQLLRTRDGDDGECK 281

Query: 250 NDFLQFLLQIKD-EGDSKTPLTMVQIKGVLM 276
            DFLQ L+++KD E DS+ P+T+  +K VL+
Sbjct: 282 -DFLQHLMKLKDQEADSEVPITVNHVKAVLV 311

BLAST of Cp4.1LG05g02550 vs. TAIR10
Match: AT4G12310.1 (AT4G12310.1 cytochrome P450, family 706, subfamily A, polypeptide 5)

HSP 1 Score: 321.6 bits (823), Expect = 5.1e-88
Identity = 161/280 (57.50%), Postives = 208/280 (74.29%), Query Frame = 1

Query: 2   IFRRR-RLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSP 61
           IF+R  +  LPPGP G+P++GNLPFLDP+LHTYFT+LA+ +GPI KL LGSK+ +++NSP
Sbjct: 35  IFKRSPQPPLPPGPRGLPIVGNLPFLDPDLHTYFTKLAQSHGPIFKLNLGSKLTVVVNSP 94

Query: 62  SVAREVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLD 121
           S+A E+LKD DI F+NHDVP  ARA TYGG D+VW PYG EWRMLRKVC  K+ S  +LD
Sbjct: 95  SLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRKVCAAKLFSRKTLD 154

Query: 122 SVYELRRREVRNTVAHLYRRA--GSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAA 181
           S YELRR+E+R     LY++    S VN+GEQ FLT+ N++ +MLWGGSV  ++ + +  
Sbjct: 155 SFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNMLWGGSVKAEDMESVGT 214

Query: 182 EFRELISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMIN--QRLRM 241
           EF+ +IS+IT LLG PNVSDFFP LARFDLQG+ K+M   A  LD I ++ I   QRLR 
Sbjct: 215 EFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYARDLDAILDRAIEQMQRLRS 274

Query: 242 ADGDEKQNKNDFLQFLLQIKD-EGDSKTPLTMVQIKGVLM 276
            DGD+ + K DFLQ L++++D E DS  P+TM  +K VLM
Sbjct: 275 RDGDDGECK-DFLQHLMKLRDQEADSDVPITMNHVKAVLM 313

BLAST of Cp4.1LG05g02550 vs. TAIR10
Match: AT4G12300.1 (AT4G12300.1 cytochrome P450, family 706, subfamily A, polypeptide 4)

HSP 1 Score: 314.3 bits (804), Expect = 8.2e-86
Identity = 154/277 (55.60%), Postives = 205/277 (74.01%), Query Frame = 1

Query: 2   IFRRR-RLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSP 61
           IF+R  +  LPPGP G+P++GNLPFLDP+LHTYF  LA+ +GPI KL LGSK+ I++NSP
Sbjct: 33  IFKRSPQPSLPPGPRGLPIVGNLPFLDPDLHTYFANLAQSHGPIFKLNLGSKLTIVVNSP 92

Query: 62  SVAREVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLD 121
           S+ARE+LKD DI F+N DVP   RAATYGG DIVW+PYG EWR LRK+C +K+LS  +LD
Sbjct: 93  SLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPYGAEWRQLRKICVLKLLSRKTLD 152

Query: 122 SVYELRRREVRNTVAHLYR--RAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAA 181
           S YELRR+EVR    +LY   R  S V +G+Q FLT+ N+  +MLWGGSV  +E + +  
Sbjct: 153 SFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTMMNLTMNMLWGGSVKAEEMESVGT 212

Query: 182 EFRELISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMAD 241
           EF+ +IS+IT LL +PNVSDFFP LARFDLQG+ K+M   A +LD + ++ I Q   +  
Sbjct: 213 EFKGVISEITRLLSEPNVSDFFPWLARFDLQGLVKRMGVCARELDAVLDRAIEQMKPLRG 272

Query: 242 GDEKQNKNDFLQFLLQIKD-EGDSKTPLTMVQIKGVL 275
            D+ + K DFLQ+L+++KD EGDS+ P+T+  +K +L
Sbjct: 273 RDDDEVK-DFLQYLMKLKDQEGDSEVPITINHVKALL 308

BLAST of Cp4.1LG05g02550 vs. TAIR10
Match: AT5G44620.1 (AT5G44620.1 cytochrome P450, family 706, subfamily A, polypeptide 3)

HSP 1 Score: 303.9 bits (777), Expect = 1.1e-82
Identity = 151/274 (55.11%), Postives = 196/274 (71.53%), Query Frame = 1

Query: 4   RRRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVA 63
           +RR   LPPGP G+P++GNLPFL PELHTYF  LA+K+GPI KL LG+K+ I++ S  VA
Sbjct: 40  KRRSPPLPPGPWGLPIIGNLPFLQPELHTYFQGLAKKHGPIFKLWLGAKLTIVVTSSEVA 99

Query: 64  REVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVY 123
           +E+LK +DI FANHDVP      TYGG +I+WSPYGP+WRMLRK+C  ++L NA LDS  
Sbjct: 100 QEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGPKWRMLRKLCVNRILRNAMLDSST 159

Query: 124 ELRRREVRNTVAHL--YRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFR 183
           +LRRRE R TV +L    R GS VN+GEQ FL + N+VT MLWG +V  +ER+ + AEF 
Sbjct: 160 DLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNVVTQMLWGTTVKEEEREVVGAEFL 219

Query: 184 ELISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDE 243
           E+I ++  LL  PN+SDFFP L+RFDLQG+ K+M + A ++D +F+++INQRL M     
Sbjct: 220 EVIREMNDLLLVPNISDFFPVLSRFDLQGLAKRMRRPAQRMDQMFDRIINQRLGMDRDSS 279

Query: 244 KQNKNDFLQFLLQIKDEGDSKTPLTMVQIKGVLM 276
                DFL  LL++KDE   KT LTM  +K VLM
Sbjct: 280 DGRAVDFLDVLLKVKDEEAEKTKLTMNDVKAVLM 313

BLAST of Cp4.1LG05g02550 vs. TAIR10
Match: AT4G12330.1 (AT4G12330.1 cytochrome P450, family 706, subfamily A, polypeptide 7)

HSP 1 Score: 291.2 bits (744), Expect = 7.4e-79
Identity = 151/284 (53.17%), Postives = 203/284 (71.48%), Query Frame = 1

Query: 10  LPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREVLKD 69
           LPPGP G+P++GNLPFL PELHTYF  LA+K+GP+ KL LG+K+ I+I S    R++L+ 
Sbjct: 46  LPPGPRGLPIVGNLPFLHPELHTYFHSLAQKHGPVFKLWLGAKLTIVITSSEATRDILRT 105

Query: 70  HDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLD--SVYELRR 129
           +D+ FAN DVP A   +TYGG DIVWSPYGPEW MLRK+C  KMLSNA+LD  S   LRR
Sbjct: 106 NDVIFANDDVPVAGSLSTYGGVDIVWSPYGPEWPMLRKICINKMLSNATLDSNSFSALRR 165

Query: 130 REVRNTVAHL--YRRAGSAVNIGEQGFLTVFNIVTSMLWGGSV-DGDERDGIAAEFRELI 189
           +E R TV +L    RAG AVN+GEQ F+T+ N+VT MLWG +V D +ER+ + AEF ELI
Sbjct: 166 QETRRTVRYLADRARAGLAVNVGEQIFVTILNVVTQMLWGETVADDEEREKVGAEFLELI 225

Query: 190 SDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDEKQN 249
           ++I  ++GKPNVSDFFP L+RFDLQG+ K++ + A ++D +F+++I+QR+ M D   K N
Sbjct: 226 TEIIDVVGKPNVSDFFPVLSRFDLQGLAKRVRRSAQRMDRMFDRIISQRMGM-DKGSKGN 285

Query: 250 KNDFLQFLLQIKDEGDSKTPLTMVQIKGVLMTVEKFGIVLKMKT 289
             DFL  LL  KDE ++   ++M  +K +LM +   G    + T
Sbjct: 286 GGDFLMVLLNAKDEDEN---MSMNHVKALLMDMVLGGTDTSLNT 325

BLAST of Cp4.1LG05g02550 vs. NCBI nr
Match: gi|449459696|ref|XP_004147582.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 465.3 bits (1196), Expect = 8.1e-128
Identity = 227/274 (82.85%), Postives = 249/274 (90.88%), Query Frame = 1

Query: 3   FRRRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSV 62
           F RRR+ LPPGPPGVPLLGNLPFLDPELH+YFTEL RKYGPIV+LQLG KIGII+NS S+
Sbjct: 38  FSRRRIHLPPGPPGVPLLGNLPFLDPELHSYFTELGRKYGPIVRLQLGGKIGIIVNSSSL 97

Query: 63  AREVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSV 122
           AREVLKDHDITFANHDV  A RAATYGG DI W+PYGPEWRMLRKVCT+KMLSNASLDSV
Sbjct: 98  AREVLKDHDITFANHDVHEAGRAATYGGSDISWAPYGPEWRMLRKVCTIKMLSNASLDSV 157

Query: 123 YELRRREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDE-RDGIAAEFR 182
           YELRRREVRNTVAHLYRRAGSAVN+GEQGFLT+FN+VTSMLWGGSV+ D+ RD IAAEFR
Sbjct: 158 YELRRREVRNTVAHLYRRAGSAVNVGEQGFLTIFNVVTSMLWGGSVESDQSRDNIAAEFR 217

Query: 183 ELISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDE 242
           E IS+IT LLG+PNVSDFFP LARFDLQGIEKQM+K  LKLD IFEKMI++R+RM D +E
Sbjct: 218 ETISEITKLLGQPNVSDFFPSLARFDLQGIEKQMVKHVLKLDTIFEKMIDERVRMKDVNE 277

Query: 243 KQNKNDFLQFLLQIKDEGDSKTPLTMVQIKGVLM 276
              KNDFLQFLL +KDEGDSKTPLTMVQ+KG+LM
Sbjct: 278 SVKKNDFLQFLLTVKDEGDSKTPLTMVQLKGLLM 311

BLAST of Cp4.1LG05g02550 vs. NCBI nr
Match: gi|659073578|ref|XP_008437137.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo])

HSP 1 Score: 453.8 bits (1166), Expect = 2.5e-124
Identity = 221/272 (81.25%), Postives = 246/272 (90.44%), Query Frame = 1

Query: 5   RRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAR 64
           +RR+ LPPGP GVPLLGNLPFLDPELH+YFTEL RKYGPIV+L+LG KIGII+NS SVAR
Sbjct: 40  QRRIHLPPGPTGVPLLGNLPFLDPELHSYFTELGRKYGPIVRLRLGGKIGIIVNSSSVAR 99

Query: 65  EVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYE 124
           EVLKDHDITFAN DVP+A RAATYGGYDI W+PYGPEWRMLRKVCT+KMLSNASLDSVYE
Sbjct: 100 EVLKDHDITFANRDVPQAGRAATYGGYDISWAPYGPEWRMLRKVCTVKMLSNASLDSVYE 159

Query: 125 LRRREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDE-RDGIAAEFREL 184
           LRRREVR TVAHLYRRAGSAVN+GEQGFLT+FN+VTSMLWGGSV+ D+ RD IAA+FRE 
Sbjct: 160 LRRREVRKTVAHLYRRAGSAVNVGEQGFLTIFNVVTSMLWGGSVENDQSRDDIAADFRET 219

Query: 185 ISDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDEKQ 244
           +SD+T LLG PNVSDFFP LARFDLQGIEKQM K   KLD IFEKMI++RLRM D +E  
Sbjct: 220 VSDVTKLLGLPNVSDFFPSLARFDLQGIEKQMGKHVQKLDAIFEKMIDERLRMKDVNESA 279

Query: 245 NKNDFLQFLLQIKDEGDSKTPLTMVQIKGVLM 276
            K+DFLQFLL++KDEGDSKTPLTMVQ+KG+LM
Sbjct: 280 KKDDFLQFLLKVKDEGDSKTPLTMVQLKGLLM 311

BLAST of Cp4.1LG05g02550 vs. NCBI nr
Match: gi|659073580|ref|XP_008437138.1| (PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo])

HSP 1 Score: 436.4 bits (1121), Expect = 4.1e-119
Identity = 209/273 (76.56%), Postives = 244/273 (89.38%), Query Frame = 1

Query: 5   RRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAR 64
           R R+ LPPGP GVPLLGNLPFLDPELHTYF +L++KYGPIVKLQLG KIGIIINSPSV R
Sbjct: 34  RLRVPLPPGPRGVPLLGNLPFLDPELHTYFAQLSQKYGPIVKLQLGRKIGIIINSPSVVR 93

Query: 65  EVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYE 124
           EVLKDHD+TFAN DVP A RAA+YGG DIVW+PYGP+WRMLRKVC +KMLSNA+LDSVYE
Sbjct: 94  EVLKDHDVTFANRDVPHAGRAASYGGSDIVWTPYGPQWRMLRKVCVLKMLSNATLDSVYE 153

Query: 125 LRRREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRELI 184
           LRRREVRNTVAHLY RAG+ VN+GEQGFLTVFN+VTSMLWGGSV+G++RDG+AAEFRE +
Sbjct: 154 LRRREVRNTVAHLYARAGTRVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETV 213

Query: 185 SDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDEKQN 244
           S++T LLG PNVSDFFP LARFD+QGIEK+M +LA + D+IFEKMI+QRL++   DE ++
Sbjct: 214 SEMTELLGLPNVSDFFPSLARFDIQGIEKKMRELAPRFDSIFEKMIDQRLKIDGKDEGES 273

Query: 245 --KNDFLQFLLQIKDEGDSKTPLTMVQIKGVLM 276
             KNDFLQFLLQ+KD+G+SKTPLTM  +K +LM
Sbjct: 274 VKKNDFLQFLLQVKDDGESKTPLTMTHLKALLM 306

BLAST of Cp4.1LG05g02550 vs. NCBI nr
Match: gi|449459692|ref|XP_004147580.1| (PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis sativus])

HSP 1 Score: 434.5 bits (1116), Expect = 1.5e-118
Identity = 210/273 (76.92%), Postives = 242/273 (88.64%), Query Frame = 1

Query: 5   RRRLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAR 64
           R R+ LPPGP GVPLLGNLPFL PELHTYF +L +KYGPIVKLQLG KIGIIINSPSV R
Sbjct: 34  RLRVPLPPGPRGVPLLGNLPFLHPELHTYFAQLGQKYGPIVKLQLGRKIGIIINSPSVVR 93

Query: 65  EVLKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYE 124
           EVLKDHD+TFAN DVP+A RAA+YGG DIVW+PYGP+WRMLRKVC +KMLSNA+LDSVYE
Sbjct: 94  EVLKDHDVTFANRDVPQAGRAASYGGSDIVWTPYGPKWRMLRKVCVVKMLSNATLDSVYE 153

Query: 125 LRRREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRELI 184
           LRR+EVRNTVAHLY RAG+AVN+GEQGFLTVFN+VTSMLWGGSV+G++RDG+AAEFRE +
Sbjct: 154 LRRKEVRNTVAHLYARAGTAVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETV 213

Query: 185 SDITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDE--K 244
           S++T LLG PNVSDFFP LARFDLQGIEK+M +LA + D+IFEKMI+QRL++  GDE   
Sbjct: 214 SEMTELLGLPNVSDFFPSLARFDLQGIEKKMRELAPRFDSIFEKMIDQRLKIDGGDEGGS 273

Query: 245 QNKNDFLQFLLQIKDEGDSKTPLTMVQIKGVLM 276
             KNDFLQFLLQ+KDE +SKTPLTM  +K +LM
Sbjct: 274 VKKNDFLQFLLQVKDEEESKTPLTMTHLKALLM 306

BLAST of Cp4.1LG05g02550 vs. NCBI nr
Match: gi|778699925|ref|XP_011654786.1| (PREDICTED: flavonoid 3',5'-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 409.5 bits (1051), Expect = 5.3e-111
Identity = 203/299 (67.89%), Postives = 241/299 (80.60%), Query Frame = 1

Query: 7   RLQLPPGPPGVPLLGNLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREV 66
           RLQLPPGP GVPLLGNLPFLDP LHTYF EL +KYG IVKLQLG K+GII+NSPSVARE+
Sbjct: 39  RLQLPPGPRGVPLLGNLPFLDPNLHTYFMELGQKYGSIVKLQLGGKVGIIVNSPSVAREI 98

Query: 67  LKDHDITFANHDVPRAARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYELR 126
           LKDHDITFAN DVP+A R ATYGG+DI W+PYGPEWRMLRKVCT+K+L NASLD VYELR
Sbjct: 99  LKDHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKLLGNASLDMVYELR 158

Query: 127 RREVRNTVAHLYRRAGSAVNIGEQGFLTVFNIVTSMLWGGSVDGDERDGIAAEFRELISD 186
           R EVR TVA LY+RA S V IGEQ F TVFN++TSMLWGG+++G+E+  +A  FRE++S 
Sbjct: 159 RSEVRKTVAQLYQRAESTVKIGEQVFFTVFNVITSMLWGGTMEGEEKAAVAVVFREMVSA 218

Query: 187 ITLLLGKPNVSDFFPRLARFDLQGIEKQMLKLALKLDNIFEKMINQRLRMADGDE-KQNK 246
           +T L GKPN+SDFFP LA  D+QGIEK+MLKL  KLD IFEK+I++RLRM + +E  +NK
Sbjct: 219 MTELAGKPNISDFFPSLACLDVQGIEKKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNK 278

Query: 247 NDFLQFLLQIKDEGDSKTPLTMVQIKGVLM------------TVEKFGIVLKMKTPLVA 293
           NDFLQFLL++KDE DS+TPLT+VQ+K +LM            T+E F +   MK P VA
Sbjct: 279 NDFLQFLLKVKDESDSQTPLTVVQLKALLMDMVFGGTDTSSNTIE-FAMAEMMKNPKVA 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C76BA_SWEMU1.4e-3933.46Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1[more]
C76B6_CATRO7.1e-3933.71Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1[more]
C75A2_SOLME5.1e-3729.80Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1[more]
CYPH1_SALMI5.1e-3733.33Ferruginol synthase OS=Salvia miltiorrhiza GN=CYP76AH1 PE=1 SV=1[more]
C7BL2_LACSA7.3e-3631.72Costunolide synthase OS=Lactuca sativa GN=CYP71BL2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KKS3_CUCSA5.7e-12882.85Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159620 PE=3 SV=1[more]
A0A0A0KR55_CUCSA1.1e-11876.92Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159600 PE=3 SV=1[more]
A0A0A0KP59_CUCSA3.7e-11167.89Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159610 PE=3 SV=1[more]
A0A059CH32_EUCGR4.8e-10368.42Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02011 PE=3 SV=1[more]
U5GNW2_POPTR5.4e-10266.42Cytochrome P450 family protein OS=Populus trichocarpa GN=POPTR_0001s07850g PE=3 ... [more]
Match NameE-valueIdentityDescription
AT4G12320.18.5e-9159.04 cytochrome P450, family 706, subfamily A, polypeptide 6[more]
AT4G12310.15.1e-8857.50 cytochrome P450, family 706, subfamily A, polypeptide 5[more]
AT4G12300.18.2e-8655.60 cytochrome P450, family 706, subfamily A, polypeptide 4[more]
AT5G44620.11.1e-8255.11 cytochrome P450, family 706, subfamily A, polypeptide 3[more]
AT4G12330.17.4e-7953.17 cytochrome P450, family 706, subfamily A, polypeptide 7[more]
Match NameE-valueIdentityDescription
gi|449459696|ref|XP_004147582.1|8.1e-12882.85PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus][more]
gi|659073578|ref|XP_008437137.1|2.5e-12481.25PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo][more]
gi|659073580|ref|XP_008437138.1|4.1e-11976.56PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo][more]
gi|449459692|ref|XP_004147580.1|1.5e-11876.92PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis sativus][more]
gi|778699925|ref|XP_011654786.1|5.3e-11167.89PREDICTED: flavonoid 3',5'-hydroxylase-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006040 amino sugar metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0033772 flavonoid 3',5'-hydroxylase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0008762 UDP-N-acetylmuramate dehydrogenase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g02550.1Cp4.1LG05g02550.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 3..276
score: 5.2
IPR001128Cytochrome P450PFAMPF00067p450coord: 11..276
score: 5.8
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 2..276
score: 7.33
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 154..172
score: 1.8E-6coord: 38..57
score: 1.8E-6coord: 62..83
score: 1.
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 6..275
score: 2.0E
NoneNo IPR availablePANTHERPTHR24298:SF114CYTOCHROME P450 - LIKE PROTEIN-RELATEDcoord: 6..275
score: 2.0E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG05g02550Cp4.1LG13g02220Cucurbita pepo (Zucchini)cpecpeB226