Cp4.1LG04g08300 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG04g08300
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Description4-hydroxy-3-methylbut-2-enyl diphosphate reductase
LocationCp4.1LG04 : 3135670 .. 3137897 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGACAGAGAGAGGAAACAGTTGCATTGAAATCAATGGAGCAGGAAGACGAAGAAGAAGAAGTGGCCGTTGTCTGAGCTTGTTACTGGAAATCGAGTTCTCAGACGATTCTCTTGCATGGTTTTCTCTCTGCAACTCTATCATCTTGCAACGCGCACTGATGCCGCCTTGCCGGAGAGTTTCGCCGGAGTTCGGTCAGTCCGGTGCAGGAAGCCTCTGTCCGTGAGATGCGATGGCGATTCTTCTTCTTCTTCTTCCTCTTTGGTGGCTGCTTATTCCAAATTTGACGCGAAGGTGTTCCGGAAGAACTTGACGAGGAGCGAGAATTATAATCGCAAAGGGTTTGGTTACAAGGACGAGACTCTGGCGCTCATGAATCGCGAGTATACGAGTGAGCCTCGATTTTCTTGACTTTGTTAAATCGTTAATGGAATTTATGAAAAGGATTATGAATTCCTTGTTTTTCTTTTGCATGCTCCATTTTGTGGCAAAACTGAAGAATACCTGCTCCTTATGAAATTTAGTCTTCCGATTTGACTTCGCTCAAGGCTAAATTGAAGAGAAAAAGCTTAATGTTTTCTAAAAGATTTTGTGCGTAAGCCTGTTTGTTCGAATTTCAGGTGATATCATAAAGACTCTCAAAGAAAATGGATTTGAATATAGGTGGGGAAATGTTACTGTAAAACTGGCGGAAGCATATGGATTTTGTTGGGGAGTTGAGCGAGCAGTCCAGATTGCTTATGAAGCGAGAAAACAATTTCCTGATGAGAAAATATGGTTACAAACTGAATGTAAGTTACCCTACAGATTTCAGCTCTAATCCCTTCTAGTTTCTTGGTTCACTTTTCAAATTGGCATTACATTATAAAGCCCACCTTTTCTTTCCTTGATTACTCATCATTTTTCATCCTTGTGAATCCATGTTAGCATGGGGAGTTAGTTGAGAAGGAGAATTGGCTACCCAAGGGTCCTATCACAATCGGTGTTACATCCGGTGCCTCGACCCCCGATAAGGTTAGCCATACACTTCGGCTTATATGCTTGCTGGTACATATTTGATCTGCAATAGATTTATAACCATAACATGTCATACGCGTTTGACTAGGTTGTCGAAGATGCTCTTGTCAAAGTCTTCGACATTAAGCGTGAAGAAGCATTGCAGGTTGCATAAATTTATAGGTTAGTAGTTCAAATTGACTTGTTAGAGACAAGAGTTGCATGATCGTAAGTGTAACTAAAGGTGATACCTTCCCTTCATCAATCATATACCCTCTTGTATCAAAATAGTAATGTTGTGCAGTAAAGATCTTAGTCCTCACAGAAATGAATTACATAGTAATGAGTACATCAAGTATGTCGACCTTGAAAACCGTTGGATCGTACCTGCAGTTGTATTGTTTGGACTTTCGAGTCGATTATTCACCCTCAAATTAGCGATATTTTCTTATAATAAATGGCGTACATGGCTACCTATGTTACATTATATACAAAATTTATTTGACTTGGAACATGGCGGTGATGAAAGAATTATAAATTGGTAAAACCGTCCTCTATATGTATATGTAAGAAAGAAGACAGCCAACTGTGATCTCAGGCCAAAATTTTGTAGTTGGACACTGTGTTTCTCTCTTGTTCCATTTTTGTCTTAGTAACTAGGCATCATTGTTATTGAACTGATTGTGAGATGGTTCGATGATTTCTGGAACACGATCGAGTAAACTGTCAATCTCCTCTGGAAATTCGACCTTTGCACGGTTCGTCTTTCCTCCGACGATCACCACTGTTCCGTCTTGTATGGCCCATATCTTTCAATCAAAGAAATGGTAAGTACACCATGTTATCTTTTTCTAGCATTGAGCATTAATAATAGGTTGAAGAAAAGCATATTTACCTGTGAATGAGAGAGAAAACTGATACATGTTGTGAGCATTAGAGCACCAAATCCAGCATAAACAACAGGCACTCCTGGGTCAGTCTGGAGATAAATAGGCAAATATCAATGACAGTTTAGAATCCAGTTTTGACACACCAGCTAAAAACTAGTGAACGTTTCCGAGTCGTTACCTTCAACTCCAGCCCAGTGCTGCCAATTGCATCAAGTATTTCAATTTTCATACCGTCGATATCGATTGGAAGCTTAGAGCTTGGACGTCGAACCCCGACAAACTTCCCTTCCTGATCATACAGGACAATGGATTGCAGATCACGAGCAAGCATTGATCTGAAAT

mRNA sequence

GAAGACAGAGAGAGGAAACAGTTGCATTGAAATCAATGGAGCAGGAAGACGAAGAAGAAGAAGTGGCCGTTGTCTGAGCTTGTTACTGGAAATCGAGTTCTCAGACGATTCTCTTGCATGGTTTTCTCTCTGCAACTCTATCATCTTGCAACGCGCACTGATGCCGCCTTGCCGGAGAGTTTCGCCGGAGTTCGGTCAGTCCGGTGCAGGAAGCCTCTGTCCGTGAGATGCGATGGCGATTCTTCTTCTTCTTCTTCCTCTTTGGTGGCTGCTTATTCCAAATTTGACGCGAAGGTGTTCCGGAAGAACTTGACGAGGAGCGAGAATTATAATCGCAAAGGGTTTGGTTACAAGGACGAGACTCTGGCGCTCATGAATCGCGAGTATACGAGTGATATCATAAAGACTCTCAAAGAAAATGGATTTGAATATAGGTGGGGAAATGTTACTGTAAAACTGGCGGAAGCATATGGATTTTGTTGGGGAGTTGAGCGAGCAGTCCAGATTGCTTATGAAGCGAGAAAACAATTTCCTGATGAGAAAATATGGTTACAAACTGAATTTGAGAAGGAGAATTGGCTACCCAAGGGTCCTATCACAATCGGTGTTACATCCGGTGCCTCGACCCCCGATAAGGTTGTCGAAGATGCTCTTGTCAAAGTCTTCGACATTAAGCGTGAAGAAGCATTGCAGGTTGCATAAATTTATAGGTTAGTAGTTCAAATTGACTTGTTAGAGACAAGAGTTGCATGATCGTAAGTGTAACTAAAGGTGATACCTTCCCTTCATCAATCATATACCCTCTTGTATCAAAATAGTAATGTTGTGCAGTAAAGATCTTAGTCCTCACAGAAATGAATTACATAGTAATGAGTACATCAAGTATGTCGACCTTGAAAACCGTTGGATCGTACCTGCAGTTGTATTGTTTGGACTTTCGAGTCGATTATTCACCCTCAAATTAGCGATATTTTCTTATAATAAATGGCGTACATGGCTACCTATGTTACATTATATACAAAATTTATTTGACTTGGAACATGGCGGTGATGAAAGAATTATAAATTGGTAAAACCGTCCTCTATATGTATATGTAAGAAAGAAGACAGCCAACTGTGATCTCAGGCCAAAATTTTGTAGTTGGACACTGTGTTTCTCTCTTGTTCCATTTTTGTCTTAGTAACTAGGCATCATTGTTATTGAACTGATTGTGAGATGGTTCGATGATTTCTGGAACACGATCGAGTAAACTGTCAATCTCCTCTGGAAATTCGACCTTTGCACGGTTCGTCTTTCCTCCGACGATCACCACTGTTCCGTCTTGTATGGCCCATATCTTTCAATCAAAGAAATGGTAAGTACACCATGTTATCTTTTTCTAGCATTGAGCATTAATAATAGGTTGAAGAAAAGCATATTTACCTGTGAATGAGAGAGAAAACTGATACATGTTGTGAGCATTAGAGCACCAAATCCAGCATAAACAACAGGCACTCCTGGGTCAGTCTGGAGATAAATAGGCAAATATCAATGACAGTTTAGAATCCAGTTTTGACACACCAGCTAAAAACTAGTGAACGTTTCCGAGTCGTTACCTTCAACTCCAGCCCAGTGCTGCCAATTGCATCAAGTATTTCAATTTTCATACCGTCGATATCGATTGGAAGCTTAGAGCTTGGACGTCGAACCCCGACAAACTTCCCTTCCTGATCATACAGGACAATGGATTGCAGATCACGAGCAAGCATTGATCTGAAAT

Coding sequence (CDS)

ATGGTTTTCTCTCTGCAACTCTATCATCTTGCAACGCGCACTGATGCCGCCTTGCCGGAGAGTTTCGCCGGAGTTCGGTCAGTCCGGTGCAGGAAGCCTCTGTCCGTGAGATGCGATGGCGATTCTTCTTCTTCTTCTTCCTCTTTGGTGGCTGCTTATTCCAAATTTGACGCGAAGGTGTTCCGGAAGAACTTGACGAGGAGCGAGAATTATAATCGCAAAGGGTTTGGTTACAAGGACGAGACTCTGGCGCTCATGAATCGCGAGTATACGAGTGATATCATAAAGACTCTCAAAGAAAATGGATTTGAATATAGGTGGGGAAATGTTACTGTAAAACTGGCGGAAGCATATGGATTTTGTTGGGGAGTTGAGCGAGCAGTCCAGATTGCTTATGAAGCGAGAAAACAATTTCCTGATGAGAAAATATGGTTACAAACTGAATTTGAGAAGGAGAATTGGCTACCCAAGGGTCCTATCACAATCGGTGTTACATCCGGTGCCTCGACCCCCGATAAGGTTGTCGAAGATGCTCTTGTCAAAGTCTTCGACATTAAGCGTGAAGAAGCATTGCAGGTTGCATAA

Protein sequence

MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYGFCWGVERAVQIAYEARKQFPDEKIWLQTEFEKENWLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREEALQVA
Homology
[Close]
BLAST of Cp4.1LG04g08300 vs. Swiss-Prot
Match: ISPH_ARATH (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana GN=ISPH PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.2e-47
Identity = 95/149 (63.76%), Postives = 114/149 (76.51%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPES-FAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAK 60
           M  +LQ   L  R D  + E+  +G  S+R RK LSVRC     ++ S  V   S FDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  VFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYG 120
           VFRKNLTRS+NYNRKGFG+K+ETL LMNREYTSDI++TLK NG+ Y WG+VTVKLA+AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPDEKIWLQTE 149
           FCWGVERAVQIAYEARKQFP+E++W+  E
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNE 149

[Close]
BLAST of Cp4.1LG04g08300 vs. Swiss-Prot
Match: ISPH_ORYSJ (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica GN=ISPH PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.8e-43
Identity = 96/176 (54.55%), Postives = 118/176 (67.05%), Query Frame = 1 

Query: 10  LATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAKVFRKNLTRSE 69
           + T+  +AL    A       R P SVRCD  ++SS S+  +  + FD K FR NLTRS+
Sbjct: 4   ITTQLRSALLSPAASPSRRARRAPSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSD 63

Query: 70  NYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYGFCWGVERAVQ 129
           NYNRKGFG+K ETL LM++EYTSD+IKTLKENG ++ WG VTVKLAEAYGFCWGVERAVQ
Sbjct: 64  NYNRKGFGHKKETLELMSQEYTSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQ 123

Query: 130 IAYEARKQFPDEKIWLQTEFEKENWLPKGPITIGVTSGASTPDKVVEDALVKVFDI 186
           IAYEARKQFPD++IWL  E      + K    +GV         +  DA +K FD+
Sbjct: 124 IAYEARKQFPDDRIWLTNEIIHNPTVNKRLEDMGV-------QNIPVDAGIKDFDV 172

[Close]
BLAST of Cp4.1LG04g08300 vs. Swiss-Prot
Match: ISPH_CYAA5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain ATCC 51142) GN=ispH PE=3 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.6e-26
Identity = 50/92 (54.35%), Postives = 72/92 (78.26%), Query Frame = 1 

Query: 57  DAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAE 116
           D K F+++L +S NY+RKGFG++ E +  MN EY S +I+ ++EN ++++ G+VT+KLAE
Sbjct: 2   DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61

Query: 117 AYGFCWGVERAVQIAYEARKQFPDEKIWLQTE 149
           A+GFCWGVERAV +AYE R+ FP EKIW+  E
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQEKIWITNE 93

[Close]
BLAST of Cp4.1LG04g08300 vs. Swiss-Prot
Match: ISPH_SYNP2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=ispH PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.7e-25
Identity = 51/92 (55.43%), Postives = 68/92 (73.91%), Query Frame = 1 

Query: 57  DAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAE 116
           D K F+++L  SENY R+GFG+KDE   +M  EY S +I+ +++N +E   G+VT+ LAE
Sbjct: 2   DTKAFKRSLNHSENYYRQGFGHKDEVAGMMTSEYQSSLIQEIRDNNYELTRGDVTIYLAE 61

Query: 117 AYGFCWGVERAVQIAYEARKQFPDEKIWLQTE 149
           A+GFCWGVERAV +AYE RK FP EKIW+  E
Sbjct: 62  AFGFCWGVERAVAMAYETRKHFPTEKIWVTNE 93

[Close]
BLAST of Cp4.1LG04g08300 vs. Swiss-Prot
Match: ISPH_MICAN (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Microcystis aeruginosa (strain NIES-843) GN=ispH PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.2e-25
Identity = 49/92 (53.26%), Postives = 70/92 (76.09%), Query Frame = 1 

Query: 57  DAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAE 116
           D K F++ L +S+NYNRKGFG+K+E +  M  EY SD+I+ ++EN +  + G+VT+ LA+
Sbjct: 2   DTKSFKRTLQQSDNYNRKGFGHKEEVMDAMTNEYQSDLIQEIRENNYRLQRGDVTIYLAQ 61

Query: 117 AYGFCWGVERAVQIAYEARKQFPDEKIWLQTE 149
           A+GFCWGVERAV +AYE R+ FP E+IW+  E
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQERIWITNE 93

[Close]
BLAST of Cp4.1LG04g08300 vs. TrEMBL
Match: K7NBR0_SIRGR (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Siraitia grosvenorii PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.7e-64
Identity = 128/153 (83.66%), Postives = 137/153 (89.54%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSL-----VAAYSK 60
           M FSLQLY L+TRTDAALPE+F+G+R+VR RKPLSVRCDGDSSSSSSS      VA  S 
Sbjct: 1   MAFSLQLYRLSTRTDAALPENFSGIRAVRYRKPLSVRCDGDSSSSSSSSSSSSSVAMDSN 60

Query: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLA 120
           FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKE+ +EY WG+VTVKLA
Sbjct: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKEHEYEYTWGDVTVKLA 120

Query: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWLQTE 149
           EAYGFCWGVERAVQIAYEARKQFPDEKIW+  E
Sbjct: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWITNE 153

[Close]
BLAST of Cp4.1LG04g08300 vs. TrEMBL
Match: A0A0A0K3D8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G075680 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 8.2e-64
Identity = 125/151 (82.78%), Postives = 134/151 (88.74%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSL---VAAYSKFD 60
           M FS+QLY L TRTDAA+PE+F  VRS+R RKP+SVRCDGDSSSSSSS     A  S FD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEA 120
           AKVFR+NLT+S+NYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEY WG+VTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLQTE 149
           YGFCWGVERAVQIAYEARKQFPDEKIWL  E
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNE 151

[Close]
BLAST of Cp4.1LG04g08300 vs. TrEMBL
Match: A0A0M4K5E2_MORAL (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Morus alba PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 8.8e-58
Identity = 115/148 (77.70%), Postives = 126/148 (85.14%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAKV 60
           M  + QL  + TRTD  LPE+F    S+RCRKPLS+RC GDSSS+SS  VAA S FDAKV
Sbjct: 1   MAIAFQLCRIPTRTDLFLPETF----SLRCRKPLSLRCSGDSSSASSPAVAAESDFDAKV 60

Query: 61  FRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYGF 120
           FRKNLTRS+NYNRKGFG+K+ETL LMNREYTSD+IKTLKENG EY WGNVTVKLAEAYGF
Sbjct: 61  FRKNLTRSKNYNRKGFGHKEETLELMNREYTSDVIKTLKENGNEYTWGNVTVKLAEAYGF 120

Query: 121 CWGVERAVQIAYEARKQFPDEKIWLQTE 149
           CWGVERAVQIAYEARKQFPDEKIW+  E
Sbjct: 121 CWGVERAVQIAYEARKQFPDEKIWITNE 144

[Close]
BLAST of Cp4.1LG04g08300 vs. TrEMBL
Match: F6HQJ5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g02030 PE=3 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.1e-57
Identity = 115/148 (77.70%), Postives = 126/148 (85.14%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAKV 60
           M  SLQL   +T +D +LPE+FAG+   R RKPLSVRC G+S SSSSS VA  S FDAKV
Sbjct: 1   MAMSLQLCRFSTFSDRSLPEAFAGIGVFRRRKPLSVRCSGESESSSSSSVAVDSDFDAKV 60

Query: 61  FRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYGF 120
           FR NLTRS+NYNRKGFG+KDETL LMNREYTSDIIKTLKENG EY+WGNVTVKLAEAYGF
Sbjct: 61  FRHNLTRSKNYNRKGFGHKDETLELMNREYTSDIIKTLKENGNEYKWGNVTVKLAEAYGF 120

Query: 121 CWGVERAVQIAYEARKQFPDEKIWLQTE 149
           CWGVERAVQIAYEARKQFP+EKIW+  E
Sbjct: 121 CWGVERAVQIAYEARKQFPEEKIWITNE 148

[Close]
BLAST of Cp4.1LG04g08300 vs. TrEMBL
Match: W9R118_9ROSA (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Morus notabilis GN=L484_026961 PE=3 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.5e-57
Identity = 115/148 (77.70%), Postives = 125/148 (84.46%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAKV 60
           M  + QL  + TRTD  LPE+F    S+RCRKPLSVRC GDSSS+SS  VA  S FDAKV
Sbjct: 37  MAIAFQLCRIPTRTDLFLPETF----SLRCRKPLSVRCSGDSSSASSPFVAVESDFDAKV 96

Query: 61  FRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYGF 120
           FRKNLTRS+NYNRKGFG+K+ETL LMNREYTSDIIKTLKENG EY WGNVTVKLAE+YGF
Sbjct: 97  FRKNLTRSKNYNRKGFGHKEETLELMNREYTSDIIKTLKENGNEYTWGNVTVKLAESYGF 156

Query: 121 CWGVERAVQIAYEARKQFPDEKIWLQTE 149
           CWGVERAVQIAYEARKQFPDEKIW+  E
Sbjct: 157 CWGVERAVQIAYEARKQFPDEKIWITNE 180

[Close]
BLAST of Cp4.1LG04g08300 vs. TAIR10
Match: AT4G34350.1 (AT4G34350.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase)

HSP 1 Score: 191.0 bits (484), Expect = 6.7e-49
Identity = 95/149 (63.76%), Postives = 114/149 (76.51%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPES-FAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAK 60
           M  +LQ   L  R D  + E+  +G  S+R RK LSVRC     ++ S  V   S FDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  VFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYG 120
           VFRKNLTRS+NYNRKGFG+K+ETL LMNREYTSDI++TLK NG+ Y WG+VTVKLA+AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPDEKIWLQTE 149
           FCWGVERAVQIAYEARKQFP+E++W+  E
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNE 149

[Close]
BLAST of Cp4.1LG04g08300 vs. NCBI nr
Match: gi|343466165|gb|AEM42976.1| (4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Siraitia grosvenorii])

HSP 1 Score: 253.8 bits (647), Expect = 2.4e-64
Identity = 128/153 (83.66%), Postives = 137/153 (89.54%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSL-----VAAYSK 60
           M FSLQLY L+TRTDAALPE+F+G+R+VR RKPLSVRCDGDSSSSSSS      VA  S 
Sbjct: 1   MAFSLQLYRLSTRTDAALPENFSGIRAVRYRKPLSVRCDGDSSSSSSSSSSSSSVAMDSN 60

Query: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLA 120
           FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKE+ +EY WG+VTVKLA
Sbjct: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKEHEYEYTWGDVTVKLA 120

Query: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWLQTE 149
           EAYGFCWGVERAVQIAYEARKQFPDEKIW+  E
Sbjct: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWITNE 153

[Close]
BLAST of Cp4.1LG04g08300 vs. NCBI nr
Match: gi|778725300|ref|XP_004137008.2| (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativus])

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-63
Identity = 125/151 (82.78%), Postives = 134/151 (88.74%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSL---VAAYSKFD 60
           M FS+QLY L TRTDAA+PE+F  VRS+R RKP+SVRCDGDSSSSSSS     A  S FD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEA 120
           AKVFR+NLT+S+NYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEY WG+VTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLQTE 149
           YGFCWGVERAVQIAYEARKQFPDEKIWL  E
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNE 151

[Close]
BLAST of Cp4.1LG04g08300 vs. NCBI nr
Match: gi|659109783|ref|XP_008454877.1| (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 249.2 bits (635), Expect = 5.8e-63
Identity = 124/148 (83.78%), Postives = 134/148 (90.54%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAKV 60
           M FS+QLY L TRTDAALPE+ + VRS+R RKP+SVRCDGDSSSSSS+ V   S FDAKV
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGDSSSSSSAAVD--SDFDAKV 60

Query: 61  FRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYGF 120
           FR+NLT+S+NYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEY WG+VTVKLAEAYGF
Sbjct: 61  FRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGF 120

Query: 121 CWGVERAVQIAYEARKQFPDEKIWLQTE 149
           CWGVERAVQIAYEARKQFPDEKIWL  E
Sbjct: 121 CWGVERAVQIAYEARKQFPDEKIWLTNE 146

[Close]
BLAST of Cp4.1LG04g08300 vs. NCBI nr
Match: gi|927030534|gb|ALD62477.1| (4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Morus alba])

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-57
Identity = 115/148 (77.70%), Postives = 126/148 (85.14%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAKV 60
           M  + QL  + TRTD  LPE+F    S+RCRKPLS+RC GDSSS+SS  VAA S FDAKV
Sbjct: 1   MAIAFQLCRIPTRTDLFLPETF----SLRCRKPLSLRCSGDSSSASSPAVAAESDFDAKV 60

Query: 61  FRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYGF 120
           FRKNLTRS+NYNRKGFG+K+ETL LMNREYTSD+IKTLKENG EY WGNVTVKLAEAYGF
Sbjct: 61  FRKNLTRSKNYNRKGFGHKEETLELMNREYTSDVIKTLKENGNEYTWGNVTVKLAEAYGF 120

Query: 121 CWGVERAVQIAYEARKQFPDEKIWLQTE 149
           CWGVERAVQIAYEARKQFPDEKIW+  E
Sbjct: 121 CWGVERAVQIAYEARKQFPDEKIWITNE 144

[Close]
BLAST of Cp4.1LG04g08300 vs. NCBI nr
Match: gi|225428566|ref|XP_002284659.1| (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Vitis vinifera])

HSP 1 Score: 231.1 bits (588), Expect = 1.6e-57
Identity = 115/148 (77.70%), Postives = 126/148 (85.14%), Query Frame = 1 

Query: 1   MVFSLQLYHLATRTDAALPESFAGVRSVRCRKPLSVRCDGDSSSSSSSLVAAYSKFDAKV 60
           M  SLQL   +T +D +LPE+FAG+   R RKPLSVRC G+S SSSSS VA  S FDAKV
Sbjct: 1   MAMSLQLCRFSTFSDRSLPEAFAGIGVFRRRKPLSVRCSGESESSSSSSVAVDSDFDAKV 60

Query: 61  FRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYRWGNVTVKLAEAYGF 120
           FR NLTRS+NYNRKGFG+KDETL LMNREYTSDIIKTLKENG EY+WGNVTVKLAEAYGF
Sbjct: 61  FRHNLTRSKNYNRKGFGHKDETLELMNREYTSDIIKTLKENGNEYKWGNVTVKLAEAYGF 120

Query: 121 CWGVERAVQIAYEARKQFPDEKIWLQTE 149
           CWGVERAVQIAYEARKQFP+EKIW+  E
Sbjct: 121 CWGVERAVQIAYEARKQFPEEKIWITNE 148

The following BLAST results are available for this feature:
BLAST of Cp4.1LG04g08300 vs. Swiss-Prot
Match NameE-valueIdentityDescription
ISPH_ARATH1.2e-4763.764-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis... [more]
ISPH_ORYSJ1.8e-4354.554-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ... [more]
ISPH_CYAA52.6e-2654.354-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain ATC... [more]
ISPH_SYNP21.7e-2555.434-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ... [more]
ISPH_MICAN2.2e-2553.264-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Microcystis aeruginosa (st... [more]
BLAST of Cp4.1LG04g08300 vs. TrEMBL
Match NameE-valueIdentityDescription
K7NBR0_SIRGR1.7e-6483.664-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Siraitia grosvenorii PE=2 ... [more]
A0A0A0K3D8_CUCSA8.2e-6482.78Uncharacterized protein OS=Cucumis sativus GN=Csa_7G075680 PE=3 SV=1[more]
A0A0M4K5E2_MORAL8.8e-5877.704-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Morus alba PE=2 SV=1[more]
F6HQJ5_VITVI1.1e-5777.70Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g02030 PE=3 SV=... [more]
W9R118_9ROSA1.5e-5777.704-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Morus notabilis GN=L484_02... [more]
BLAST of Cp4.1LG04g08300 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G34350.16.7e-4963.76 4-hydroxy-3-methylbut-2-enyl diphosphate reductase[more]
BLAST of Cp4.1LG04g08300 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|343466165|gb|AEM42976.1|2.4e-6483.664-hydroxy-3-methylbut-2-enyl diphosphate reductase [Siraitia grosvenorii][more]
gi|778725300|ref|XP_004137008.2|1.2e-6382.78PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cu... [more]
gi|659109783|ref|XP_008454877.1|5.8e-6383.78PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-lik... [more]
gi|927030534|gb|ALD62477.1|1.3e-5777.704-hydroxy-3-methylbut-2-enyl diphosphate reductase [Morus alba][more]
gi|225428566|ref|XP_002284659.1|1.6e-5777.70PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Vi... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:00517454-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
GO:0046872metal ion binding
Vocabulary: Biological Process
TermDefinition
GO:0050992dimethylallyl diphosphate biosynthetic process
GO:0019288isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Vocabulary: INTERPRO
TermDefinition
IPR003451LytB/IspH
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050992 dimethylallyl diphosphate biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0051745 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g08300.1Cp4.1LG04g08300.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductasePFAMPF02401LYTBcoord: 112..145
score: 1.
NoneNo IPR availablePANTHERPTHR31619FAMILY NOT NAMEDcoord: 1..148
score: 9.2
NoneNo IPR availablePANTHERPTHR31619:SF24-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTICcoord: 1..148
score: 9.2

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG04g08300CmaCh19G006460Cucurbita maxima (Rimu)cmacpeB539
Cp4.1LG04g08300CmoCh11G013260Cucurbita moschata (Rifu)cmocpeB126
Cp4.1LG04g08300CmoCh19G006580Cucurbita moschata (Rifu)cmocpeB494
Cp4.1LG04g08300Cla015963Watermelon (97103) v1cpewmB665
Cp4.1LG04g08300MELO3C018407Melon (DHL92) v3.5.1cpemeB604
Cp4.1LG04g08300ClCG02G005330Watermelon (Charleston Gray)cpewcgB590
Cp4.1LG04g08300Lsi11G010600Bottle gourd (USVL1VR-Ls)cpelsiB533
Cp4.1LG04g08300MELO3C018407.2Melon (DHL92) v3.6.1cpemedB715
Cp4.1LG04g08300Bhi05G001046Wax gourdcpewgoB0855
Cp4.1LG04g08300Carg22032Silver-seed gourdcarcpeB0296
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG04g08300Cp4.1LG15g05170Cucurbita pepo (Zucchini)cpecpeB269
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG04g08300Cucurbita pepo (Zucchini)cpecpeB500
Cp4.1LG04g08300Cucumber (Gy14) v1cgycpeB0602
Cp4.1LG04g08300Cucurbita maxima (Rimu)cmacpeB154
Cp4.1LG04g08300Wild cucumber (PI 183967)cpecpiB671
Cp4.1LG04g08300Wild cucumber (PI 183967)cpecpiB697
Cp4.1LG04g08300Cucumber (Chinese Long) v2cpecuB668
Cp4.1LG04g08300Cucumber (Chinese Long) v2cpecuB695
Cp4.1LG04g08300Bottle gourd (USVL1VR-Ls)cpelsiB525
Cp4.1LG04g08300Cucumber (Gy14) v2cgybcpeB237
Cp4.1LG04g08300Cucumber (Gy14) v2cgybcpeB969
Cp4.1LG04g08300Melon (DHL92) v3.6.1cpemedB746
Cp4.1LG04g08300Silver-seed gourdcarcpeB0932
Cp4.1LG04g08300Cucumber (Chinese Long) v3cpecucB0835
Cp4.1LG04g08300Cucumber (Chinese Long) v3cpecucB0868
Cp4.1LG04g08300Wax gourdcpewgoB0864