Cp4.1LG04g07470 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG04g07470
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionHemK methyltransferase family member 2
LocationCp4.1LG04 : 4152815 .. 4153522 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCCCATTCAAAACTGCGCAAATCCACCTTGTGAGTTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCATTTGCTTTGGTTGATGCACTTTTAGCTGACCGAGTTAACTTGTTAAATCATCACCCAACATTGTGCTTAGAAGTAGGCTGTGGAAGTGGGTATGTCATAACATCTCTTGCTCTTATGTTGGGAAAGGAAGCCTCTGGCACTCACTATATTGCTACAGACATCAATCCTCATGCAACGAGAGTCACTCAGGAGACACTAGAAGCACATGGGGTTCATGCGGAGTTGATATCCACTGACATAGCATATGGTTTGGAGAAACGACTGGCTTCATCTGTTGATGTGTTGATTGTGAATCCACCCTATGTTCCAACCCCTGAGGATGAAGTGGGTCTTGAAGGAATTGCCTCTGCTTGGGCAGGAGGGGAAAATGGCCGCAGCGTTATCGATAGAATGTTGCCTGTTGCTGATGCTCTTTTGTCTGACAAAGGCTGGTTGTACATGGTTTTTCTTGAAGCAAACAATCCCTCCCAGATATGCCTTCAAATGAGAGAAAAAGGGTATGCTAGTCGAATCGTTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTTATCAAGTTTTGGAAGAATGCTGATCTTCAAGTTCATGGAAAAGATTCAAGTCACAAAACAGGAACAATTTCAACATCTTAA

mRNA sequence

ATGTCCCCATTCAAAACTGCGCAAATCCACCTTGTGAGTTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCATTTGCTTTGGTTGATGCACTTTTAGCTGACCGAGTTAACTTGTTAAATCATCACCCAACATTGTGCTTAGAAGTAGGCTGTGGAAGTGGGTATGTCATAACATCTCTTGCTCTTATGTTGGGAAAGGAAGCCTCTGGCACTCACTATATTGCTACAGACATCAATCCTCATGCAACGAGAGTCACTCAGGAGACACTAGAAGCACATGGGGTTCATGCGGAGTTGATATCCACTGACATAGCATATGGTTTGGAGAAACGACTGGCTTCATCTGTTGATGTGTTGATTGTGAATCCACCCTATGTTCCAACCCCTGAGGATGAAGTGGGTCTTGAAGGAATTGCCTCTGCTTGGGCAGGAGGGGAAAATGGCCGCAGCGTTATCGATAGAATGTTGCCTGTTGCTGATGCTCTTTTGTCTGACAAAGGCTGGTTGTACATGGTTTTTCTTGAAGCAAACAATCCCTCCCAGATATGCCTTCAAATGAGAGAAAAAGGGTATGCTAGTCGAATCGTTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTTATCAAGTTTTGGAAGAATGCTGATCTTCAAGTTCATGGAAAAGATTCAAGTCACAAAACAGGAACAATTTCAACATCTTAA

Coding sequence (CDS)

ATGTCCCCATTCAAAACTGCGCAAATCCACCTTGTGAGTTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCATTTGCTTTGGTTGATGCACTTTTAGCTGACCGAGTTAACTTGTTAAATCATCACCCAACATTGTGCTTAGAAGTAGGCTGTGGAAGTGGGTATGTCATAACATCTCTTGCTCTTATGTTGGGAAAGGAAGCCTCTGGCACTCACTATATTGCTACAGACATCAATCCTCATGCAACGAGAGTCACTCAGGAGACACTAGAAGCACATGGGGTTCATGCGGAGTTGATATCCACTGACATAGCATATGGTTTGGAGAAACGACTGGCTTCATCTGTTGATGTGTTGATTGTGAATCCACCCTATGTTCCAACCCCTGAGGATGAAGTGGGTCTTGAAGGAATTGCCTCTGCTTGGGCAGGAGGGGAAAATGGCCGCAGCGTTATCGATAGAATGTTGCCTGTTGCTGATGCTCTTTTGTCTGACAAAGGCTGGTTGTACATGGTTTTTCTTGAAGCAAACAATCCCTCCCAGATATGCCTTCAAATGAGAGAAAAAGGGTATGCTAGTCGAATCGTTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTTATCAAGTTTTGGAAGAATGCTGATCTTCAAGTTCATGGAAAAGATTCAAGTCACAAAACAGGAACAATTTCAACATCTTAA

Protein sequence

MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS
Homology
[Close]
BLAST of Cp4.1LG04g07470 vs. Swiss-Prot
Match: HEMK2_HUMAN (HemK methyltransferase family member 2 OS=Homo sapiens GN=N6AMT1 PE=1 SV=3)

HSP 1 Score: 172.2 bits (435), Expect = 6.9e-42
Identity = 91/199 (45.73%), Postives = 120/199 (60.30%), Query Frame = 1 

Query: 17  EVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIA 76
           +VYEP +D+F L++AL A    L      +CLEVG GSG V   LA M+G +A    Y+ 
Sbjct: 21  DVYEPAEDTFLLLNALEAAAAELAGVE--ICLEVGSGSGVVSAFLASMIGPQAL---YMC 80

Query: 77  TDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNPPYVPTPEDEVGL 136
           TDINP A   T ET   + VH + + TD+  GL  RL   VD+L+ NPPYV TP  EVG 
Sbjct: 81  TDINPEAAACTLETARCNKVHIQPVITDLVKGLLPRLTEKVDLLVFNPPYVVTPPQEVGS 140

Query: 137 EGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRI 196
            GI +AWAGG+NGR V+DR  P+   LLS KG  Y+V ++ NNP +I   M+ KG     
Sbjct: 141 HGIEAAWAGGKNGREVMDRFFPLVPDLLSPKGLFYLVTIKENNPEEILKIMKTKGLQGTT 200

Query: 197 VVQRSTEEESLHVIKFWKN 216
            + R   +E+L V+KF K+
Sbjct: 201 ALSRQAGQETLSVLKFTKS 214

[Close]
BLAST of Cp4.1LG04g07470 vs. Swiss-Prot
Match: MTQ2_SCHPO (eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=mtq2 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.6e-22
Identity = 73/221 (33.03%), Postives = 114/221 (51.58%), Query Frame = 1 

Query: 6   TAQIHLVSSHREVYEPCDDSFALVDALLADRVNL---LNHHPTLCLEVGCGSGYVITSLA 65
           T+Q+ L    ++VYEP +D+FAL+DAL  D   L         L  E+GCGSG   + L 
Sbjct: 7   TSQLRL-KEFQDVYEPAEDTFALLDALEKDAKKLRQMAEMKNLLTAEIGCGSGCASSFLK 66

Query: 66  LMLGKEASGTHYIATDINPHATRVTQET-LEAHGVHAE------LISTDIAYGLEKRLAS 125
             + K     H+++ DI+  A R ++ T L    ++ +       + T    G+  RL +
Sbjct: 67  SGILKNKPIVHFMS-DISNSACRASKITALNNRELYKDDNGLFITVQTSFLDGI--RLGN 126

Query: 126 SVDVLIVNPPYVPTPEDEVGLEG--IASAWAGGENGRSVIDRMLPVADALLSDKGWLYMV 185
            VD+LI NPPYVPT  +E+  E   IASAWAGG +G  V   +L     +LS  G  YMV
Sbjct: 127 GVDILIFNPPYVPTEFEEIPSEAATIASAWAGGTDGMDVTSTLLNQLKDILSQDGVFYMV 186

Query: 186 FLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWK 215
            +  N    IC  +++ G+     ++R    E+L +++ ++
Sbjct: 187 AVARNKLHSICEILQKDGFIVNETLKRKAGRETLSILRIYR 223

[Close]
BLAST of Cp4.1LG04g07470 vs. Swiss-Prot
Match: Y928_METJA (Putative protein methyltransferase MJ0928 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) GN=MJ0928 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 8.5e-16
Identity = 55/200 (27.50%), Postives = 94/200 (47.00%), Query Frame = 1 

Query: 15  HREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHY 74
           H EVYEP +DS  L+        NL++      LE+G G+G +  + A    K+  G   
Sbjct: 13  HPEVYEPAEDSILLLK-------NLVDVKNKDVLEIGVGTGLISIACAKKGAKKIVGV-- 72

Query: 75  IATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNPPYVPTPEDEV 134
              DINP+A ++ +E  + + V+     +D    L + +    DV++ NPPY+PT EDE 
Sbjct: 73  ---DINPYAVKLAKENAKLNNVNISFFESD----LFENVTGKFDVILFNPPYLPTSEDEK 132

Query: 135 GLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYAS 194
               +  A+ GG++GR ++DR +      L   G + ++        +   +++  G+  
Sbjct: 133 IDSYLNFAFDGGKDGREILDRFIYELPNYLKKGGVVQILQSSLTGEKETINKLKPLGFKV 192

Query: 195 RIVVQRSTEEESLHVIKFWK 215
            I  +     E L VI  W+
Sbjct: 193 EISARLKVPFEELMVINAWR 196

[Close]
BLAST of Cp4.1LG04g07470 vs. Swiss-Prot
Match: MTQ2_YEAST (eRF1 methyltransferase catalytic subunit MTQ2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MTQ2 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.2e-15
Identity = 56/180 (31.11%), Postives = 87/180 (48.33%), Query Frame = 1 

Query: 17  EVYEPCDDSFALVDALLADRVNL---LNHHPTLCLEVGCGSGYVITSLALMLGKEASGTH 76
           +VYEP +DSF ++D L  +   L     +   +  E+G GSG V T L          + 
Sbjct: 13  KVYEPAEDSFLILDCLEKEHDFLKQKFGNRLAIVCEIGSGSGIVTTFLMQNKIIPQENSI 72

Query: 77  YIATDINPHATRVTQETLEAHGVHA---ELISTDIAYGLEKRLASSVDVLIVNPPYVPTP 136
           ++A DINP A   T +T + +   +   E+I  D+   +     + VDVLI NPPYVP  
Sbjct: 73  HLAVDINPWALEATLDTAKLNSCKSSFLEVIQADLNSSIRN---NQVDVLIFNPPYVPAE 132

Query: 137 --EDEVGLEGIASAW-----AGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQI 184
              D  G    A  W      GG++G ++ D++L   + +LS  G  Y++F   N P ++
Sbjct: 133 CVPDVPGSREEADQWLDLALLGGKDGMAITDKLLRQLEQILSPDGVAYILFCARNKPKEV 189

[Close]
BLAST of Cp4.1LG04g07470 vs. Swiss-Prot
Match: Y1784_ARCFU (Putative protein methyltransferase AF_1784 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) GN=AF_1784 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.7e-13
Identity = 56/197 (28.43%), Postives = 93/197 (47.21%), Query Frame = 1 

Query: 18  VYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIAT 77
           +YEP +DS  L++A L +            +EVG GSG+V   L      +      + T
Sbjct: 2   IYEPAEDSELLLEAALREL-----KPDDEVIEVGAGSGFVAERL------KGKCKCILTT 61

Query: 78  DINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNPPYVPTPEDEVGLE 137
           DI+P A       +E      +++ TDIA G+ K+ +    +++ NPPYV   ++    +
Sbjct: 62  DISPFAA------MELRRKGLDVVMTDIAKGIRKKFS----LVLFNPPYVELEDELRRGD 121

Query: 138 GIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRIV 197
            +  A  GG+ G  VI + L   D +++++G   ++    N P    L + E+G+   IV
Sbjct: 122 WLDVAIDGGKKGVKVIKKFLDSLDEIMAERGRAILIASSQNEPDVFDL-IDERGFRYEIV 176

Query: 198 VQRSTEEESLHVIKFWK 215
            +R    E L  IK WK
Sbjct: 182 GERGLFFEKLFAIKIWK 176

[Close]
BLAST of Cp4.1LG04g07470 vs. TrEMBL
Match: E5GCR1_CUCME (N6-DNA-methyltransferase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 9.5e-115
Identity = 205/230 (89.13%), Postives = 216/230 (93.91%), Query Frame = 1 

Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MS F+TAQIHLVSSHREVYEPCDDSFALVDALLADRV LLNH+PTLCLE GCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LAL+LGKEAS THYIATDINPHA RVTQETLEAHG+HAEL+ TD+A GLEKRLA SVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           +VNPPYVPTPEDEVG +GIASAWAGGENGRSVIDR+LPVAD LLSDKGWLYMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG 231
            QICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GKDS+HKTG
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTG 230

[Close]
BLAST of Cp4.1LG04g07470 vs. TrEMBL
Match: A0A0A0K7J9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067460 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 1.6e-114
Identity = 205/230 (89.13%), Postives = 216/230 (93.91%), Query Frame = 1 

Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MS F+TAQI LVSSHREVYEPCDDSFALVDALLAD+V LLNHHPTLCLE GCGSGYVITS
Sbjct: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LAL+LGKEAS THYIATDINPHA RVTQETLEAHG+HAEL+ TDIA GLEKRLA SVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           +VNPPYVPTPEDEVG +GIASAWAGGENGRSVIDR+LPVAD LLSDKGWLYMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG 231
           +QICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GKDS+HKTG
Sbjct: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTG 230

[Close]
BLAST of Cp4.1LG04g07470 vs. TrEMBL
Match: I1JKQ0_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_03G027700 PE=4 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 8.0e-98
Identity = 175/222 (78.83%), Postives = 197/222 (88.74%), Query Frame = 1 

Query: 5   KTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALM 64
           +TAQIHLVSSH+EVYEPCDDSFALVDALLADR NLL H PTLC+E+GCGSGYVITSLAL+
Sbjct: 9   RTAQIHLVSSHQEVYEPCDDSFALVDALLADRNNLLEHRPTLCMEIGCGSGYVITSLALL 68

Query: 65  LGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNP 124
           LG+E  G +YIATDINPHA RVT ETLEAHGV AELI TDIA GLE RLA  VDV++VNP
Sbjct: 69  LGQEGCGINYIATDINPHAVRVTHETLEAHGVGAELIVTDIASGLEDRLAGLVDVIVVNP 128

Query: 125 PYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQIC 184
           PYVPTPEDEVG+EGI S+WAGGENGRSVID++LPVAD LLS+KGWLYMV L ANNPS+IC
Sbjct: 129 PYVPTPEDEVGVEGIMSSWAGGENGRSVIDKILPVADRLLSEKGWLYMVTLTANNPSEIC 188

Query: 185 LQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSS 227
            QMR+KGYAS+IVVQRSTEEESLH+IKFW++ D + +  D S
Sbjct: 189 HQMRKKGYASKIVVQRSTEEESLHIIKFWRDFDTEANETDQS 230

[Close]
BLAST of Cp4.1LG04g07470 vs. TrEMBL
Match: A0A0B2RUZ4_GLYSO (HemK methyltransferase family member 2 OS=Glycine soja GN=glysoja_030984 PE=4 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 8.0e-98
Identity = 175/222 (78.83%), Postives = 197/222 (88.74%), Query Frame = 1 

Query: 5   KTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALM 64
           +TAQIHLVSSH+EVYEPCDDSFALVDALLADR NLL H PTLC+E+GCGSGYVITSLAL+
Sbjct: 9   RTAQIHLVSSHQEVYEPCDDSFALVDALLADRNNLLEHRPTLCMEIGCGSGYVITSLALL 68

Query: 65  LGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNP 124
           LG+E  G +YIATDINPHA RVT ETLEAHGV AELI TDIA GLE RLA  VDV++VNP
Sbjct: 69  LGQEGCGINYIATDINPHAVRVTHETLEAHGVGAELIVTDIASGLEDRLAGLVDVIVVNP 128

Query: 125 PYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQIC 184
           PYVPTPEDEVG+EGI S+WAGGENGRSVID++LPVAD LLS+KGWLYMV L ANNPS+IC
Sbjct: 129 PYVPTPEDEVGVEGIMSSWAGGENGRSVIDKILPVADRLLSEKGWLYMVTLTANNPSEIC 188

Query: 185 LQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSS 227
            QMR+KGYAS+IVVQRSTEEESLH+IKFW++ D + +  D S
Sbjct: 189 HQMRKKGYASKIVVQRSTEEESLHIIKFWRDFDTEANETDQS 230

[Close]
BLAST of Cp4.1LG04g07470 vs. TrEMBL
Match: K7K3R1_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.4e-97
Identity = 174/222 (78.38%), Postives = 197/222 (88.74%), Query Frame = 1 

Query: 5   KTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALM 64
           +TAQIHLVSSH+EVYEPCDDSFALVDALLADR NLL HHPTLC+E+GCGSGYVITSLAL+
Sbjct: 647 RTAQIHLVSSHQEVYEPCDDSFALVDALLADRNNLLEHHPTLCMEIGCGSGYVITSLALI 706

Query: 65  LGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNP 124
           LG+E  G +YIATDINPHA RVT ETLEAHGV AELI TDIA GLE RLA  VDV++VNP
Sbjct: 707 LGQEVCGINYIATDINPHAVRVTHETLEAHGVGAELIVTDIASGLEDRLAGLVDVMVVNP 766

Query: 125 PYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQIC 184
           PYVPTPEDEVG+EGI S+WAGGENGRSVI+++LPVAD LLS+KGWLYMV L  NNPS+IC
Sbjct: 767 PYVPTPEDEVGVEGITSSWAGGENGRSVINKILPVADRLLSEKGWLYMVTLTENNPSEIC 826

Query: 185 LQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSS 227
            QMR+KGYAS+IVVQRSTEEESLH+IKFW++ D + +  D S
Sbjct: 827 HQMRKKGYASKIVVQRSTEEESLHIIKFWRDFDTEANETDQS 868

[Close]
BLAST of Cp4.1LG04g07470 vs. TAIR10
Match: AT3G13440.1 (AT3G13440.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 334.0 bits (855), Expect = 7.7e-92
Identity = 157/224 (70.09%), Postives = 190/224 (84.82%), Query Frame = 1 

Query: 3   PFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLA 62
           P + A I LVSSHREVYEPCDDSFALVDALLADR NL+ H+P +C+E+GCGSGYVITSL 
Sbjct: 34  PPRIAAIRLVSSHREVYEPCDDSFALVDALLADRTNLIEHNPKICMEIGCGSGYVITSLI 93

Query: 63  LMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIV 122
           L+L  E  G HY+A D NP ATRVT+ETLEAHGV+A++I  D+A GLEKRLA SVDV++V
Sbjct: 94  LLLQNEVPGVHYLAIDTNPIATRVTKETLEAHGVNADVICADLATGLEKRLAGSVDVIVV 153

Query: 123 NPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQ 182
           NPPYVPTPE EVG+EGIASAWAGGENGR+VID++LPV D LLS+KGW Y+V L +N P++
Sbjct: 154 NPPYVPTPEYEVGMEGIASAWAGGENGRTVIDKILPVVDLLLSEKGWFYLVTLTSNFPAE 213

Query: 183 ICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSS 227
           ICL MR+KGYASRIVVQRSTEEE+L ++KFW++ D +   K++S
Sbjct: 214 ICLMMRKKGYASRIVVQRSTEEENLVILKFWRDKDEESQDKETS 257

[Close]
BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: gi|659110217|ref|XP_008455111.1| (PREDICTED: hemK methyltransferase family member 2 [Cucumis melo])

HSP 1 Score: 421.0 bits (1081), Expect = 1.4e-114
Identity = 205/230 (89.13%), Postives = 216/230 (93.91%), Query Frame = 1 

Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MS F+TAQIHLVSSHREVYEPCDDSFALVDALLADRV LLNH+PTLCLE GCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LAL+LGKEAS THYIATDINPHA RVTQETLEAHG+HAEL+ TD+A GLEKRLA SVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           +VNPPYVPTPEDEVG +GIASAWAGGENGRSVIDR+LPVAD LLSDKGWLYMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG 231
            QICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GKDS+HKTG
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTG 230

[Close]
BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: gi|449438254|ref|XP_004136904.1| (PREDICTED: hemK methyltransferase family member 2 [Cucumis sativus])

HSP 1 Score: 420.2 bits (1079), Expect = 2.3e-114
Identity = 205/230 (89.13%), Postives = 216/230 (93.91%), Query Frame = 1 

Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MS F+TAQI LVSSHREVYEPCDDSFALVDALLAD+V LLNHHPTLCLE GCGSGYVITS
Sbjct: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LAL+LGKEAS THYIATDINPHA RVTQETLEAHG+HAEL+ TDIA GLEKRLA SVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           +VNPPYVPTPEDEVG +GIASAWAGGENGRSVIDR+LPVAD LLSDKGWLYMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG 231
           +QICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GKDS+HKTG
Sbjct: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTG 230

[Close]
BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: gi|719965612|ref|XP_010257002.1| (PREDICTED: hemK methyltransferase family member 2 [Nelumbo nucifera])

HSP 1 Score: 378.3 bits (970), Expect = 1.0e-101
Identity = 178/231 (77.06%), Postives = 206/231 (89.18%), Query Frame = 1 

Query: 5   KTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALM 64
           K+AQI LVSSH EVYEPCDDSFALVDALLADR NLL HHP+LC+EVGCGSGYVITSLA+M
Sbjct: 38  KSAQIRLVSSHPEVYEPCDDSFALVDALLADRTNLLEHHPSLCMEVGCGSGYVITSLAIM 97

Query: 65  LGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNP 124
           LG+E +G HYIATDINPHA +VT ETL+AHGVHAE+ISTDIA GLE+RL+  VDV++VNP
Sbjct: 98  LGQEVAGVHYIATDINPHAVQVTSETLKAHGVHAEVISTDIASGLERRLSGIVDVMVVNP 157

Query: 125 PYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQIC 184
           PYVPTPEDEVG +GI SAWAGGENGR VIDR+LP+AD LLSD+GWLYMV L ANNPSQIC
Sbjct: 158 PYVPTPEDEVGRDGITSAWAGGENGRRVIDRILPIADKLLSDRGWLYMVMLAANNPSQIC 217

Query: 185 LQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS 236
           L MREKGYASRI+VQRSTEEESLHV+KFW+++D+QV  ++ +  + T ST+
Sbjct: 218 LAMREKGYASRIIVQRSTEEESLHVVKFWRDSDIQVGAREPTTTSRTTSTT 268

[Close]
BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: gi|1009166820|ref|XP_015901791.1| (PREDICTED: hemK methyltransferase family member 2 [Ziziphus jujuba])

HSP 1 Score: 372.5 bits (955), Expect = 5.5e-100
Identity = 178/215 (82.79%), Postives = 195/215 (90.70%), Query Frame = 1 

Query: 4   FKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLAL 63
           F+TAQI LVSSH EVYEPCDDSFALVDALLADR NL+ H PTLC+EVGCGSGYVITSLAL
Sbjct: 3   FRTAQIRLVSSHPEVYEPCDDSFALVDALLADRTNLVQHQPTLCMEVGCGSGYVITSLAL 62

Query: 64  MLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVN 123
           +LGKE+ G H+IATDINPHA RVTQETLEAHGVHAELISTDIA GLE+RLA SVDV++VN
Sbjct: 63  ILGKESVGAHFIATDINPHAVRVTQETLEAHGVHAELISTDIALGLEQRLAGSVDVMVVN 122

Query: 124 PPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQI 183
           PPYVPTPEDEVG EGI SAWAGGENGR VIDR+LP+AD LLS+KGWLYMV L ANNP QI
Sbjct: 123 PPYVPTPEDEVGCEGIVSAWAGGENGRVVIDRILPIADNLLSEKGWLYMVTLAANNPIQI 182

Query: 184 CLQMREKGYASRIVVQRSTEEESLHVIKFWKNADL 219
           C  MREKGYASRIV+QRSTEEESLH+IKFW++ D+
Sbjct: 183 CRTMREKGYASRIVLQRSTEEESLHIIKFWRDFDI 217

[Close]
BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: gi|571444113|ref|XP_006576417.1| (PREDICTED: hemK methyltransferase family member 2-like [Glycine max])

HSP 1 Score: 364.8 bits (935), Expect = 1.2e-97
Identity = 175/222 (78.83%), Postives = 197/222 (88.74%), Query Frame = 1 

Query: 5   KTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALM 64
           +TAQIHLVSSH+EVYEPCDDSFALVDALLADR NLL H PTLC+E+GCGSGYVITSLAL+
Sbjct: 9   RTAQIHLVSSHQEVYEPCDDSFALVDALLADRNNLLEHRPTLCMEIGCGSGYVITSLALL 68

Query: 65  LGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNP 124
           LG+E  G +YIATDINPHA RVT ETLEAHGV AELI TDIA GLE RLA  VDV++VNP
Sbjct: 69  LGQEGCGINYIATDINPHAVRVTHETLEAHGVGAELIVTDIASGLEDRLAGLVDVIVVNP 128

Query: 125 PYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQIC 184
           PYVPTPEDEVG+EGI S+WAGGENGRSVID++LPVAD LLS+KGWLYMV L ANNPS+IC
Sbjct: 129 PYVPTPEDEVGVEGIMSSWAGGENGRSVIDKILPVADRLLSEKGWLYMVTLTANNPSEIC 188

Query: 185 LQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSS 227
            QMR+KGYAS+IVVQRSTEEESLH+IKFW++ D + +  D S
Sbjct: 189 HQMRKKGYASKIVVQRSTEEESLHIIKFWRDFDTEANETDQS 230

The following BLAST results are available for this feature:
BLAST of Cp4.1LG04g07470 vs. Swiss-Prot
Match NameE-valueIdentityDescription
HEMK2_HUMAN6.9e-4245.73HemK methyltransferase family member 2 OS=Homo sapiens GN=N6AMT1 PE=1 SV=3[more]
MTQ2_SCHPO4.6e-2233.03eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (stra... [more]
Y928_METJA8.5e-1627.50Putative protein methyltransferase MJ0928 OS=Methanocaldococcus jannaschii (stra... [more]
MTQ2_YEAST4.2e-1531.11eRF1 methyltransferase catalytic subunit MTQ2 OS=Saccharomyces cerevisiae (strai... [more]
Y1784_ARCFU6.7e-1328.43Putative protein methyltransferase AF_1784 OS=Archaeoglobus fulgidus (strain ATC... [more]
BLAST of Cp4.1LG04g07470 vs. TrEMBL
Match NameE-valueIdentityDescription
E5GCR1_CUCME9.5e-11589.13N6-DNA-methyltransferase OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0K7J9_CUCSA1.6e-11489.13Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067460 PE=4 SV=1[more]
I1JKQ0_SOYBN8.0e-9878.83Uncharacterized protein OS=Glycine max GN=GLYMA_03G027700 PE=4 SV=1[more]
A0A0B2RUZ4_GLYSO8.0e-9878.83HemK methyltransferase family member 2 OS=Glycine soja GN=glysoja_030984 PE=4 SV... [more]
K7K3R1_SOYBN1.4e-9778.38Uncharacterized protein OS=Glycine max PE=4 SV=1[more]
BLAST of Cp4.1LG04g07470 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G13440.17.7e-9270.09 S-adenosyl-L-methionine-dependent methyltransferases superfamily pro... [more]
BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659110217|ref|XP_008455111.1|1.4e-11489.13PREDICTED: hemK methyltransferase family member 2 [Cucumis melo][more]
gi|449438254|ref|XP_004136904.1|2.3e-11489.13PREDICTED: hemK methyltransferase family member 2 [Cucumis sativus][more]
gi|719965612|ref|XP_010257002.1|1.0e-10177.06PREDICTED: hemK methyltransferase family member 2 [Nelumbo nucifera][more]
gi|1009166820|ref|XP_015901791.1|5.5e-10082.79PREDICTED: hemK methyltransferase family member 2 [Ziziphus jujuba][more]
gi|571444113|ref|XP_006576417.1|1.2e-9778.83PREDICTED: hemK methyltransferase family member 2-like [Glycine max][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008276protein methyltransferase activity
GO:0008168methyltransferase activity
GO:0003676nucleic acid binding
Vocabulary: Biological Process
TermDefinition
GO:0032259methylation
Vocabulary: INTERPRO
TermDefinition
IPR007848Small_mtfrase_dom
IPR004557PrmC-related
IPR002052DNA_methylase_N6_adenine_CS
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032775 DNA methylation on adenine
biological_process GO:0006479 protein methylation
biological_process GO:0034047 regulation of protein phosphatase type 2A activity
biological_process GO:0007165 signal transduction
biological_process GO:0032259 methylation
cellular_component GO:0000159 protein phosphatase type 2A complex
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008276 protein methyltransferase activity
molecular_function GO:0019888 protein phosphatase regulator activity
molecular_function GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0019706 protein-cysteine S-palmitoyltransferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g07470.1Cp4.1LG04g07470.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002052DNA methylase, N-6 adenine-specific, conserved sitePROSITEPS00092N6_MTASEcoord: 120..126
scor
IPR004557Eukaryotic/archaeal PrmC-relatedTIGRFAMsTIGR00537TIGR00537coord: 18..213
score: 4.2
IPR007848Methyltransferase small domainPFAMPF05175MTScoord: 43..173
score: 4.1
NoneNo IPR availablePANTHERPTHR18895METHYLTRANSFERASEcoord: 5..217
score: 3.4
NoneNo IPR availablePANTHERPTHR18895:SF71HEMK METHYLTRANSFERASE FAMILY MEMBER 2coord: 5..217
score: 3.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG04g07470Cucsa.218590Cucumber (Gy14) v1cgycpeB0601
Cp4.1LG04g07470CmaCh19G005110Cucurbita maxima (Rimu)cmacpeB539
Cp4.1LG04g07470CmoCh11G014420Cucurbita moschata (Rifu)cmocpeB126
Cp4.1LG04g07470CmoCh19G005240Cucurbita moschata (Rifu)cmocpeB494
Cp4.1LG04g07470Csa7G067460Cucumber (Chinese Long) v2cpecuB694
Cp4.1LG04g07470MELO3C018575Melon (DHL92) v3.5.1cpemeB614
Cp4.1LG04g07470ClCG02G007550Watermelon (Charleston Gray)cpewcgB590
Cp4.1LG04g07470CSPI07G06260Wild cucumber (PI 183967)cpecpiB696
Cp4.1LG04g07470Lsi03G006460Bottle gourd (USVL1VR-Ls)cpelsiB543
Cp4.1LG04g07470MELO3C018575.2Melon (DHL92) v3.6.1cpemedB726
Cp4.1LG04g07470CsaV3_7G007230Cucumber (Chinese Long) v3cpecucB0867
Cp4.1LG04g07470Bhi05G000823Wax gourdcpewgoB0856
Cp4.1LG04g07470CsGy7G005890Cucumber (Gy14) v2cgybcpeB967
Cp4.1LG04g07470Carg23052Silver-seed gourdcarcpeB1033
Cp4.1LG04g07470Carg16306Silver-seed gourdcarcpeB0639
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG04g07470Cp4.1LG15g04100Cucurbita pepo (Zucchini)cpecpeB269
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG04g07470Cucumber (Chinese Long) v3cpecucB0868
Cp4.1LG04g07470Cucurbita pepo (Zucchini)cpecpeB400
Cp4.1LG04g07470Cucumber (Gy14) v1cgycpeB0602
Cp4.1LG04g07470Cucurbita maxima (Rimu)cmacpeB154
Cp4.1LG04g07470Cucurbita maxima (Rimu)cmacpeB749
Cp4.1LG04g07470Cucurbita moschata (Rifu)cmocpeB321
Cp4.1LG04g07470Cucurbita moschata (Rifu)cmocpeB703
Cp4.1LG04g07470Wild cucumber (PI 183967)cpecpiB697
Cp4.1LG04g07470Cucumber (Chinese Long) v2cpecuB695
Cp4.1LG04g07470Bottle gourd (USVL1VR-Ls)cpelsiB548
Cp4.1LG04g07470Melon (DHL92) v3.5.1cpemeB604
Cp4.1LG04g07470Cucumber (Gy14) v2cgybcpeB969
Cp4.1LG04g07470Melon (DHL92) v3.6.1cpemedB715
Cp4.1LG04g07470Melon (DHL92) v3.6.1cpemedB769
Cp4.1LG04g07470Silver-seed gourdcarcpeB0409
Cp4.1LG04g07470Silver-seed gourdcarcpeB0529