Cp4.1LG03g08420 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG03g08420
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionFolylpolyglutamate synthase
LocationCp4.1LG03 : 2849105 .. 2862298 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TATGGAAAAAAAAAACGGAAATTTAACCATCCAAATTTCATCCTCAGACACCAGTTTCAACGACAAAAAAAGTACGGACATATGTATATTATATTGCTCCAGATTCGGATTCTTGCTCATGGCGGCTTCGCGCTTCTAATCTCCCCAATCTGAGTGCAGCCCTTACTCCAGTTTCTTCGCATTTCTGCGTTTCTGTATGTATAACTCTTACTTTTTTTTTTCTTGCGCTCTACCTGTTCTTTCTGTGATTTTGTTTGTTCAATGTTTAGCTTCATCATCTACTTTACTGAATCGGTAGAGGTAGAGGTGGAGTGAGATGGAACTGCTGCTTTCTTATCCATATCAGTCGATGCTTCGTCTTGTAGTGAGAATTTGAAGAATAGGTTGAAACCTAAGTTTGAATTTGTACTTTTTTCTTGTGATGTGCAAAATTACTAAGGTTTTGTTTTCCAATCTAGCTTAACAACACACAATTCTTCTTCTTGTTCTCATTATTGTTGAATATTGATTGATTTATAATCGGCGCCTTTGGAATTTGAAAGCATTTGATCCCACTTCAAACCACTGGTCTCTAGAATAATGGGTTAGTGGTCCGAAGTTGTTCCTCACACAATCTCATTCTCAAGATATTGAATTTAGAAAGCCATCTATTCATTAGAGCTTGAAGTTCCCCCCCCTCCCNACAAAACCCGATAAACCCTTTGGTAAAGCCTTCATTTCTTTAGTTCTTCCTTTTCTCTACTTCAAAATACTACATTTTTCTTGTTGTATTATAAATTACCAATTTCTCAATATTCTCTATGCCTTGCCTCCCTGATGCTTGCACTTGCTCTTAGGTTCTAAAATACTATTACACTTTAATGGGCCTTGTGTTTTAGAAAACCCTGGATGGAATAGAATATCTTGGAGGCTATTCAGTAAAGCACCATCTCTTTCCTTCTCAATTCTCCTTTCTCACCTTTCTCTTGTTTTGTGAGGTTTGTGTACTCAGAAAGATTTGGTGTCTTCTTTGGCACTTCCTCAATATGATTTTTGATTATTGATTTCGAGTTTCCGAAAATCTTATTTGTTTTTCAATGATGTGATAGGTTTCTGAAAGGTATAAAGGCTGTTACTTGGGAAAAAAAATGAGAAACAATTTGCCTCAGTCATCTTCCTATGAATCTGCTCTGGAAGCACTTTCGTCCTTGATAACACAGAAAAGTCGTGGAACAATGGCTCCAGTAATCAGGAGCAAGACAAAATTAGAAAGGATGAGAAAGTACGTTGAGGTAACTCTTCTGACATCAGACTCTAATGAAGAAATCATGAAAGTAAAATGTTTCAACATTGCTGATCTGATATTGTGAATATGCGATAAAAAATTTGTTGGTCAATTATTTAGCTTGATTGCTCTTTTTAAATATTTACTATTGTCACAATAGAGAAGAGTGGAAATGATCATTTGAGTTTGCCTATCATACTACTTGAACCACTTTATAAAGATTAAATCACAATGCTTATGTTTCTTTTGCAGATTCTAGACCTAGTAGAATGTGTAAAGAAACTAAAAATTATTCACGTGGCTGGAACTAAGGGAAAGGTATTCTATTTTCTACCTTTAATATTGCTTATACATATTGCAGGTGAGGAATAAAACTTAGGTTAGAAGATCATTGGAGTATAGTTTATTTCATAACCTCTTTTTGCCTCAATCAATGAAAATTTTATGTTATGGTATTTATGTCTCTATAAATGAATGAAAAACAAATTTTCATCAAAGACAAATGAAAAATCCAACAAAAGAAGCCAACTAAAAAAGAAAGAAAGAAAGAAAGAAAGGAAGGAAGAAAGATGATAGGGAATAAGCCTCCCGTGTGTTTTACTTGTGCTCAAGGCAGCGTGAGCTACCCGAACAAGATAAGGAAGGAGCGAAGTAGTGTAAATTGCAATAAAGAGCATGGAGGTGTTTTTCTAATTTTTTAAGTTTAATCCTAATTTCAATTTTTTGTGTAAGTTCTTTAACATAATGTTACTGTAGGTCACATATGGGGAAAGAGAGTAAAAATATGTACAAAATAGCATGAGGTTAGGGTGGGAATAGTTTCAGGAAATAGGTAGGGGCTACTAGCTTGTTTTACTTCTTTGGGGATTTTGTATCCATTGTTAATTGAATAAGTTGAGTCTTGATTAGGAACTTTGATGGGGGAGGTACAAAGTGTTACTACCTCATAAAGGACCGTTGATGTAGCAAGAGGATTTAGAATGCATGGTCTCTGATGGTGTAGAGGGAGGGGAAAAAGGCAAACAGTTGGATATGTGATGGTTTTGGGTGTGGGGCAGTTGAGTGTAGTCGCTAATGTTTGATGTAAAGAATTATGAAGTGGATAGATTTTCGAAGCCTAACAATTCAAAATCTACAAGATGAGTAAAGAAGAGAGGAAGGGGGTGATAGTGATAGTGTTGAGTATTTCTGGATAGGCTTTAGCATGGTATTGCCTAATTGGAAAATGTCCGACTGAATAATATTTAGACCACGTTTCAATTTATAAAAAAATTATTTATGTTTTCCTGTTTTGCATCTCCCACGCTAAAATGAGGGGGAAAAAAAAAAAAACTCTTACTTTTTTTGCACCTCTCGCTTAAGTTCTAGAAGGCTGTCTCACTCTTTAAAAAACACCCGGGAAAGTATTGGTTGGGGACAAAGAACTTGAGGGGCAACTCTTGTCTGTCTTTGCCTCACTTATTCCCCATCTGTTTGTCCGAGACCGTTCTTGGAAGGTTTAAATTGGTGTTCCATTTTATTTCATTAGGGATAGGTATGGGTTTGAGACTCCTTTTAGTATGAAGGAAATCAAGAGGGCGTTTTTTTAGCTTCGATGGGAATAAGGTGCCTATCATGAGCTTCATTATGGCCTTCTCTAAGGAAAAGAAAGATATTAAGGAGGAGGAATTGGTTGTGGTTTTTATTGAATGTGATCATGGTCACTATGAATGAGACTTATATAGAACTATGCATACCTGGAGAGGGACTTTGCACTTTTTAGTCTCTTCATGATCTTCTAGCACTCGAGATGAATTTTTCGCAATCCTCTAAAGCTAGGGTTTGGATTTTGGTAATATTTCATACTATCAATGAAATATGTGTTTCCCAACAAATTCTTCTCAATGCTTGGGTTTTTTTTTTTTTTTTTTTTCTGTTAATTCCTCCTGTAATAAATTTTGTTGGTAACATTTCATTATGTCAATGAGAAGTATCATTTTTTTTTTGTAAAAAATGACATGCTATCCATATTCATTTTGCATTCATACTTTTTTCTTATCAATACAATGAATGAGTTGGCTAGTTTTCATGCTACAATTTTTTACATAAGTTTCGTCAAATAATGGAGCGAACTAAAAATGTATGAGAAAATTTGTGGGCCAAAAGATAACTATATTGTGATGGTTTAATGGCTCTCCAAAGTAAAATAAATAACTTTTGGTCCATCATTCATATCTTGCTGGTGCAGAAAAAGGAACATCAATACCTGGTTAAAGAATAAGCAGGGCCTCAGTATTGCAGTATTCTTTAATCCTCTTTTCATTTTCACCTGATCTGTATGAATTCAGGGTTCCACATGCACCTTTTCTGAAGCAATTTTACGGGAATGTGGTCTTCGAACTGGACTTTTCACTTCCCCTCATCTCATTGATGTGCGTGAGAGATTTCGTATAAATGGGTTAGTGTTGAAATATATGTTGGTATTTAGCAAAAAACATGTCTCATCTCACCCATCCCTCTCTTTCTATTATAAAACACAAACATCATGTACATGTGACACACTGAGATATTATTAGGAAGCTCTCCGTTTACTTATTATTTTCAAGCTCATTAACTTTGTTGAAGTTGTATTTCTTACTTTATCTAATATATATATATTTTTTACAATCTCACTTCGAATGGAACAATCAGATTGGATATATCAGAAGATAAATTTCTGCATTACTTCTGGGCATGCTGGAGACAATTGACGGTATTGTTGCTATTCCTTACTTCAATTCGATTCTATTTGATGCAGGCTCTAGCTAAGCTTTTTTGCACGGTCATGATTTATAAGAATTACAATGAGTATATACATCATTTGCATGTCCAGATGGTTTAGTCAACTGTAGATGTACGGGAAAGTATCAAACAAGAATACTACTTCTGCCTTAACCAACTTACCCACATTGGTACAGATCCACATACAATTGGTCCAGTTCATAATCTCTTGCATCCTCTTTGATTGTTGATGAAATGTTTTGATGAAGTTTCCAAGACAAAAACAGAAAAAAAGCTAACTTGTTGAGTTTATTTGGAATTGACGAGTCAGTCACTTGTGAAGGAAGATGCACATATATCAATCTTCTAGCATTTAGGAGGAGTGCGTTGGAGTAATAGTTGGAAACAGAGTTTGTGAAAACTTCAATATTTGAATAGTTCAATGATTCAGTTGAATTATGTGTGAATAATAAATTGATATTTTGTCTTATACTGGCTGAGATTCTAGCTTGTGCTTCAATATTCTTACTTTCTGTTCCTTACATGGCATAGCTATCTCTGGTGTATTTTCGTTTCCTAATTTTTTGGAACAAGAAACAAAATTTTCATTCATAGATGAAAAGAAAGAAAAAGATAAGGGTACAAATTACAATTCAACGATGTGTGTGCCTAATTTTTCTTTTGCCAGTATGCAAACTACAACCTTCAGTTTTGTGATTTTGGGTGTTAATTTGCAGGAAAGTGTTACAGAACATCTGCCAATGCCTGCACTATTTCAGTTTCTTACATTGTTGGCTTTTAAAATATTCATAGATGAAGAGGTAAATGACTTTAGTTTCAGTTAATATAGCTCCTATTTTAAACAGTCTAGGGGTAGTTGCAATGATTGCTTTCATTCCCTTGTTCTATTTTTGCAAGAACTTATGATTTATGCAAGAATTGAACATCATGAATGTCATGGACTTTTAATGTCATAGCATGATATATGAAGGATCTTTCGTACAATTCACTATCTTCTGTTACTAGCTTCTCTATTCCTTTCCCATATATTATTATGAATGGTGAAAGAATAGAGACGGCAGAGAAGTAGTCCTCTGCAAGCTTAAATAGGTTTCTTCAAACCTATTAGACTTTGTCCTCCCAGAAACTAATATGGCTTAGTGTTCTTTGGCATAAACTTTCTTTGACTTGGAAGAAAGCCTCGTTTCCTGAAGGGTGTTTGACTCGTTGATTTAGTCTGTCTCAGGTGGGATTTTATTATAATTTCTTTGTCGTTATGAAAAGGATAAAAATAAAGCACCAAAAGCCAAGTTCATTTCCTTTTCTTCTTTCAATGCACCATCTGGTGACCTTGCATCTTTAATGTCCATTTCTTCTTCTTCTTCTTCTATCATCATCAACACTTTGATCAATGTTTGTATATATATATATATATATATATATATATATATATATATATATTTATATATCTATAAACTTCAAATAACTCTCATGCTAAAATTTTGTTTTAGCTTATGATAAGTATTTATGATGCAATGCCACATATCAAAATTTTTGAGATATATCCAAACTAACGTCTTGCCAAAAAGGATATTTAAAGTCAATTATGATGCAAAAACACTCTATTTTAGGCAGAGTGCTCTGTTAGTTTTCATTCATTGAGTTGGAGGAATCCATAGGAGGCCCGCCGAGATGAAGAAATGGCTACTGCTAAAAGAAAAGGGAGGTCCACCTGCCTACCTAAGAAATCCGAGACCTGTTGTAGGAGAGAAGCAACTTGATTGATAGGGAGAGGAGTTTGTATAAATTCTTTTGAGATAAGTGAGATTAAATCTTGGATTATAGACTTTGGTACACTACCTTAGTTTGGATGTCATCTACATACACTAGAAGCAGTGTAGTCTTGGATTATAGACTTCGAATACCATAAGAATATTGTTATCGTTGGTTGCCGACTTAAATTGACAGCTCTCACTATGCGATGTGAAGAATACATTCCTTCACGATGATCTAGATGAGCAAAACTACATGAGTACTCCCCCAGGGTTTGAAAAAACTCGAGAAGTGTTTAGACTGAGAAAAGTCAAGGAGATCACTCTCTTTATCAAAAGCTAAGAAGCAAAGAGAGCTACAATGCTTCTAGTGTACGTAGATGACACCATAGTCACTTCAGATAACGAAGAAGAAGAGATTGAACTTGAGGGAAAGTGATGAGAGAATTCGAGATTAAAGAACTTAGAAGGCTAAAGTACTTTCTCTGTATTGAAGTCGCATACTCAGCCCAAAACATCTTTATATCACAACAAAAATATGTGATGAATTTATTGATGGAAATAAGAAAGGGCAAATGTAAACTTGCGCTAGTGCCAATTGATCCACATAAGAATTTGGAAGAGTCAAAAGAAGAACCGGTGAACAGAGAGATGTGCTGGAGGATGGTGGGGAGGCTTATATATTTAGCTCACACTCAACTAGACATAGCGTACTTGAGTGTATATTAGCCAATTTATGCACGACAAACAAGAGTCTTTCATTATTAGGGACAAATTTGGAATTTTTAAATTATAAAAAGAGTAAATATGTCCAAAATTGCTTCTCAACTATCCCACCATTTAATATAGTGTAGACGTGTTTGATAATTGTTGGCTAAATGTACATGAGTGTCCAACACGTGTGGTTTATTGAGCTTAATAAGTGTTTAACATGTGTCCAACAAGTATGGAACTTATATTCATTTTGTCAACTAATGATTTTCGTAGAATCTACTTGTTCTTTTCCTACAAAAGAAGCTCCCTCACAACCAGTTAACAACGTTTTAAACCTGATCAATTTGAGGATTAGTTTTTCTAAACATGTTTCAGTAACTTGGATTACAGAGTTACATCCTTATTTTCCTTCTTGTTGGCCTTTTTCCTTTATTTTTCATTATGGAATTATTGATTTTATCTCAATAGGTTGACGTTGCAATTATTGAAGTTGGTCTTGGGGGAATGTACGATTCAACAAATGTGGTATGCTCTGCCTATTAGAATTGGTTTATTCCAAAAGTTTTTGAGATTGAAAACTAGTAACTATGAAATCTTAAGATTAATAAAAATTGATTGATCAATTGGTGGTTATTGTGCAGATAGAAGAACCTATAGTCTGTGGCATCACTTCGCTTGGGATGGACCACATGGAGTTATTAGGCATATCTATCTTACTAGTCATGATTTTCTACCTTAAGCTTTTTGGTTAAAAATTTTTAATGTTAATTTTCGGGGAAAACCATTCAAGCATGAGATGGAGATGGCTTGCAGTATGAATGCTTGAAACAAACTAATGCTCCAATTGTTATAAATCTGGTTTGAGCATCAATGCAAAACAAAAAATGAATTACCTGTGAATTTAAGTTGGTTGCTTCGACCAGATTCTGACAGTGACACATGTTTCAGGAAACTCGCTAAGCCAGATTGCTTCGCACAAGGCTGGAATTCTCAAGGTACAGTGTCAATGTAAGCCTTTGCAATAACATGATTTTTTATTTTGTTCTAAATATGACCACCTAGGACAGGCTCTCATTGTATATCTCTGTTTGGGGGGGAGGGGTTCCCTCTACCCCTGCCATTAGGCTGTTCTTGTCCTTTTTTTGGAATATACCATTTTGATGTTTCTTGTAAAAAGAAAAATAAATATGACCACCTAGGACAGCATTCTTTCTTAGTTTAGAATCACAGTTTTGAAGAATGAAATGCACTAATATTGCACCAAGTGTTATGCATGGCTGTTGCTTATTTTTCTTTAGTGGATTAGTGCAATATTCTTCTTTGAGTATACAAAATTGCAGTTTTAAGAAAAAAAAAATATAAAGATATTTTGTGCCATGCTTGATTTTTACTCGTATTCTAAGTCAGCTTCACATCTTGTATCCATGTAAAGCCTCGAGTTCCTGCATTCACAGTACCACAGCTTCCAGAAGCAATGGATGTTATCCAGAACAAGGCACGTGAGTTGATGGTAATTTTCTTCCTTTCAAGACAAGATTCAATGCAACTCAAGCTATCTTTGGCTTATAAGCAGCTTCACGATTCTCGAGTTTGCATGTTAATACTCGTGAATTTTGAATAAAATCATGAAATGAATTTCTTTTTCAACTAGATTTTTGAGCAAACAATTAAGCAGTGAAATACTTGTAGAGCTTTCTCTGAAAACATTCAAGAATCTTGTAGTTAAGAGTTTTAATGCATGTTATAACAGTGATAAAAGCCTCGTATCATTGTGTATTGGTATTAGTAAAGCCCAGACACATTGATCAGCATTTTGTTGAATTTTCTTTGGTTTTATCAGAAGAAAGTCCTCCAGGTTAATGCTTGTCGTGTTCTCCTTCAGCACTTTACTGGATTCTACTTTGCGCTCTGGTTCTTATTGTTGTATCATGCATGAGCTCCAAGCTATTTTGTAACTATGGTTGATCTTCCATGCACACCAATTAGAGATCTTCTATGTAACTCTTTGATATCCCCTTATACTTCTGTATTTCTTTTATTCAACGAAATCATCAATAAAGCCCAAACATAGCTGAAGAGAATTTCATAGAGATAGGCTAAAATTCTACTTGCGGTAGTACAAGTTCAGCTCTCAATACTTCAAAGTATTAATAGAAATGTCTAGAATATACAATGTGCACATTTTATGTGGGGTTATGAAAACTCATGATACGGAACTTCATTGGACATTGACCTATGCTTACCGATTGCAGATCCCATTAGAGGTAGCTGAACCGCTTGACCATAAAAAGCTAGATGGATTGAAGCTTGGCATGTCTGGTGATCATCAGCTTGTTAATTCTGGTCTTGCTGTTTCCCTTTGTAAATGTTGGCTTCAGAGGACTGGAAACTGGGAAAGGATGTTCCTAAATGTTGGTCTTATCTTCATATTTTACTTTATGCTTGACATGACGTTTTTGTTGATGGTTTCTTCTTGAATTGTTGGTCGTGGCATTTAGGAAATGGGAGATTATAGTGACTGGAGGTGTTAAGTTGCTTGGTCTGTTCTTCCCCCCTCCCTGTTGACATGCGTGTTGTATATTATGCATGTGCTTCACCTGCCATACGTGCATGCATCCCCATTTATCAGAATGAAATTTGGATGATATCATAAAATGTGTTATGCGATAACTATTTTATCTAGGAACTAGAGGATTTTGTGTCTACTCTTAATCAATGTTGAGTTGATCTTTTCCCTTCAAACTGCTGCAATCTTCTTTCTTCTTTCTTCATTCTTCATTCTTCCTCTTCCTCTTCTTCTTCTTCTTCTTTTTTTTTTTTTTTCTCTTTATTATTTTTATTTTAAAATTTATGTTCTAAAACATAGATATTTAAGCCATTTCACTGTATCTGTCTTGTGCGAAAATCAGGGTTTCAAAGTGGCCAAAATACCAGATGCATTTCTTAGAGGTCTTTCAACAGCAAATCTTTCTGGGAGAGCACAGACAGTTTATGATTGTTATGCATCATCATTTAATTCTTCAACACAGTCTGAAAACTCGAATGGAGATTTAATATTCTATTTGGATGGAGCTCATAGCCCAGAAAGCATGGAGGCTTGTGCTAACTGGTTCTCTAGTGTTGTGAAGGGAAATCATAAGTCGTTACATTGTTTTAGTGCTGAAAATGTGGACAGAGATTCAGGAAATGATGATGTAAAAGTCCTGCAGGAAGCTGAGTCCACAAAGTTGTCAAAGCAGGTAATTTCCACAATTAACATTGTACTGTGGCATTTGAGATTTCTTTTCAGCGTTACTGATGAATCCCTCCCATTCCTGTTTTTTACTTCAACTTATATTGGTGACTTATTAAATTTTGATGATGTAAAAGACAGTTAAATTAATTTTTCCTGGTCTGTAGATTCTCCTATTCAATTGCATGGAAATGAGAGACCCTGAAATCTTATTTCCACGACTTGTGAATACATGTGCTTCCTCAGGTATTATGCTTGTACCCTTGTGTTTTTATTTTTTTATTCGGCTTTTATCAACCTAAATAACCCATAGATAGCAGTTATAATCACAATCCAATTTTTCTTCCCCTTACGCATAGGTGTACCTTGAGCCATGTTTTCCTCCACTTATGGTGCATAGGCCGGGGATTGAAATCTTCACAAAAGACATCCGTCGTTTTTCATTAAGATATATACATAATATGAACTTTTTCACTGTAAAAAAGCTATGCAACATGATATATTAATGATGTCATCGTCAATATTGCACATAATGCACGAAGATAACAGAAGTTGGGGATTCTTCTTTCAGATTTTATCTAAGATGTTCAACTTGTAATGGAGAAGAGTCCATGCAAGTTGCAGTAGGCAATTTAATCCATCTGGTCATCAAATTTATGTTGAGGATCTAACTGCCTTATGGCATGGTATCTTGCTGAGTGAAGAGGTCCATGGTGATTTTTGCGAAGCTGCTGATAGAATTTTAACACGCCGATCTATTTGTATGTTTAACAGTGGTTGTTCTAAAGTAGTTTTCGTTTTCAGCAGCACAATTTGAAATCTTAACATCATAAAAGGTGTTCTTCTGTTCATTATAACTGGAAAAGTGGTTAACAGGGATGCTCTCATTGATCTTACTTTCAAGTCAATTTTTGCCGGGTTTTGAGATTTTGAACCCATTTTTCTGACTTCGATCTTTCATCTTTAGGTGTTCATTTTTCGAAAGCCCTTTTTGTCCCTACCATGTCAACATATAGCAAAGTCTCTTCCAGTAATTCGGTGACTTCGTCAGATATTATAAACAAGGACTTGTCGTGGCAGCTCAACCTCCAGAAACTTTGGGAGAAGATTATGCATGGGAAGGGTATTGTTCCTTCTCTTTTTGCTTCAAGTCGCACAACAATTGAATATATTATGTTCGTTCTTTGTCTATTTTTCATATGCTGCAGTTATAACTGTCATGCATACTCCACTGAAGTGGGGAATGTTTGTTTAATTCCAATCTGGCTGCAGCTGAAATACTTTCTATGAAAAAAGAGGTGATTAACAGAAATTCTTTGAATTCTTTCATTGGAATATAATATATATTGAATCGTTACAATGATCTGCAATATGAAATAACCGATATTAGGTGCAATTGATGGAGGGAAAGTAAACTGTCAGTTCTTCAAATGAAATAAACCATTGATTAATAAGCGGTAAGTTTTAGGCTGTAGCAGGAAGTAAGTTTAAAGCTTTTTTAAAATGCTTTGTTGAAGTAAAGCTTGGATTTAGGGATGGAAAATATGTAGAAGTTATAGGTCTCACTGTACTGATTGCATTATTACAAAAATGAGAAAATAACCGACTTCCTTGGGGAAGTTCTTTAGATGGAAAATCTAAATTTCTATCGAGAAAAGAAAAATGAAAGAATACAAAATGATAAAAAAAAAAAAAAAAAACCCCACAAAAGGAGTTAAACCACCTGAACTACTATACAAATAATGAGCTCTGATCCAAAATTGTGTGAGACAACGGATAATTACAAAAGCTCTTGGAGACTAATGCTCAAAGTCGAGGAGAAGCATTAAAGCTGGCAATAGACTACACCTCTTCCTAGGATTTCCCAATTGCTTTAACAATCCTAATATTCCTCTGAATCCAAAGGCCCTGCAAAACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAAGAACCCAGCTTTCCACGAGATCTAACCCTTATCTCAAAGTGATAGATGCAAAGGAACCTCCTCCATTATATAACAACAATCTATTTGCTTGCTTGAACTGCTTGCTTTCCCCTATTTCTTCTTTCACGAGGGTTTCACTTTTGAGCCAAACAAACACCTAACGACTTTTAAAAACTCGCAGAACGAATGTGATGATGCAGCAAAGTAATGGTTGGCTGTGCATGATAACCCTCTTTGAAGTTTTTGTATTGCTATAAAAAAGTTGCAACTCACAGGCAAGATAATTATACTTAGTTCCCTAGAACTATACTTATATCTGATCATGCAGCAAAGCAATGGTTCTAGAACTATACTTATATCTGATCATGCAGCAAAGCAATGGTTCTGTTACTCAAATGATTCCACAATAGCAGTTCGTTGGACACTTTTCATGGATGAACAGCATTATAAGGCTATTGATAGCTTAATGCTAAATTCATGGAGGCTTTCCTTTTGTAAATGGTGGTTTAATATATGATTCAACTAATGATTTATGGTTTAATTGTTTTATTTGAATCTTGATCGAACATGGTTGTCATCTCATCTGCTTCTGCAGAACCAGATTCTATATCCGAAAAGGATCTTAAGCTCGACCACGAGCGAGGTTTGCCTCCTCTGTATGAGGACTTTCACTCAAGTCTGTCGGATAACAAATTCAGTGGCAGCGCAGTGATGCCTTCATTGCCATTGGCGATAAATTGGCTGAGAGATTGCGTCAGAGAATCCCCATCCATTAGACTACAGGTATGAGATATTTAAATCTCACTCTGTGAATGGCTGTGAAATTTCAGTTCCGTGATATCTTCTTTTGACCTTTTAACTCTGTTAATTAGTTTGTTCTTAAGTTGGGGATAGTCATAGGGTGATAGATATCTAAGTCATTCATTTGATCTTATTTCTGCCCTTTTCTCCATGATCAGCTTTTACTTTGTGCTTAGTTGAGTTCTTCAGAATTGTAGTTCATGCAGTTCATTATGAGTGCTGTTCTAAAAAATTAAAAAAAAAAAGGAAAAAGAAATCAAAATGTAGTTGTTTACTTGAGCAAATTTTGTGATGAATTCAGGTTCTTGTCACTGGATCCCTTCATCTGGTAGGAGATGTGCTCAAGTTAATAAAAAGGTGATGTTAGATCAACTCTTCTGATCTTCTCCCCATTGTTAGGGATGTGATATTTCAAACTTCCTAGGATCGCCTATCTGTTGGTTCACTTTTTGTTGGTTTTTATCTCACAGTTCATCGTTTTTCATTCAGTTCATACCTCAATAAATTAATTGTGGTAAATTTTATGTTATTTTCTGCTACTATCTTTGGTACTCCATTTCCTGGTGCTAGATTTTTCTTGGAAGAACAAAACTTTCCTTTCTTTCAAGATACAAATCATTTTCATTCTGGTAATTTAGTCTTCATTTGTTCAAGTGTCTGTGATGGTAATCTTGTAGAACCCTTTTTTTTTTATGTCTACATGAGAAAATAGGTT

mRNA sequence

TATGGAAAAAAAAAACGGAAATTTAACCATCCAAATTTCATCCTCAGACACCAGTTTCAACGACAAAAAAAGTACGGACATATGTATATTATATTGCTCCAGATTCGGATTCTTGCTCATGGCGGCTTCGCGCTTCTAATCTCCCCAATCTGAGTGCAGCCCTTACTCCAGTTTCTTCGCATTTCTGCGTTTCTGTTTCTGAAAGGTATAAAGGCTGTTACTTGGGAAAAAAAATGAGAAACAATTTGCCTCAGTCATCTTCCTATGAATCTGCTCTGGAAGCACTTTCGTCCTTGATAACACAGAAAAGTCGTGGAACAATGGCTCCAGTAATCAGGAGCAAGACAAAATTAGAAAGGATGAGAAAGTACGTTGAGATTCTAGACCTAGTAGAATGTGTAAAGAAACTAAAAATTATTCACGTGGCTGGAACTAAGGGAAAGGGTTCCACATGCACCTTTTCTGAAGCAATTTTACGGGAATGTGGTCTTCGAACTGGACTTTTCACTTCCCCTCATCTCATTGATGTGCGTGAGAGATTTCGTATAAATGGATTGGATATATCAGAAGATAAATTTCTGCATTACTTCTGGGCATGCTGGAGACAATTGACGGAAAGTGTTACAGAACATCTGCCAATGCCTGCACTATTTCAGTTTCTTACATTGTTGGCTTTTAAAATATTCATAGATGAAGAGGTTGACGTTGCAATTATTGAAGTTGGTCTTGGGGGAATGTACGATTCAACAAATGTGATAGAAGAACCTATAGTCTGTGGCATCACTTCGCTTGGGATGGACCACATGGAGAAACTCGCTAAGCCAGATTGCTTCGCACAAGGCTGGAATTCTCAAGTACCACAGCTTCCAGAAGCAATGGATGTTATCCAGAACAAGGCACGTGAGTTGATGATCCCATTAGAGGTAGCTGAACCGCTTGACCATAAAAAGCTAGATGGATTGAAGCTTGGCATGTCTGGTGATCATCAGCTTGTTAATTCTGGTCTTGCTGTTTCCCTTTGTAAATGTTGGCTTCAGAGGACTGGAAACTGGGAAAGGATGTTCCTAAATGGTTTCAAAGTGGCCAAAATACCAGATGCATTTCTTAGAGGTCTTTCAACAGCAAATCTTTCTGGGAGAGCACAGACAGTTTATGATTGTTATGCATCATCATTTAATTCTTCAACACAGTCTGAAAACTCGAATGGAGATTTAATATTCTATTTGGATGGAGCTCATAGCCCAGAAAGCATGGAGGCTTGTGCTAACTGGTTCTCTAGTGTTGTGAAGGGAAATCATAAGTCGTTACATTGTTTTAGTGCTGAAAATGTGGACAGAGATTCAGGAAATGATGATGTAAAAGTCCTGCAGGAAGCTGAGTCCACAAAGTTGTCAAAGCAGATTCTCCTATTCAATTGCATGGAAATGAGAGACCCTGAAATCTTATTTCCACGACTTGTGAATACATGTGCTTCCTCAGGTGTTCATTTTTCGAAAGCCCTTTTTGTCCCTACCATGTCAACATATAGCAAAGTCTCTTCCAGTAATTCGGTGACTTCGTCAGATATTATAAACAAGGACTTGTCGTGGCAGCTCAACCTCCAGAAACTTTGGGAGAAGATTATGCATGGGAAGGAACCAGATTCTATATCCGAAAAGGATCTTAAGCTCGACCACGAGCGAGGTTTGCCTCCTCTGTATGAGGACTTTCACTCAAGTCTGTCGGATAACAAATTCAGTGGCAGCGCAGTGATGCCTTCATTGCCATTGGCGATAAATTGGCTGAGAGATTGCGTCAGAGAATCCCCATCCATTAGACTACAGGTT

Coding sequence (CDS)

ATGAGAAACAATTTGCCTCAGTCATCTTCCTATGAATCTGCTCTGGAAGCACTTTCGTCCTTGATAACACAGAAAAGTCGTGGAACAATGGCTCCAGTAATCAGGAGCAAGACAAAATTAGAAAGGATGAGAAAGTACGTTGAGATTCTAGACCTAGTAGAATGTGTAAAGAAACTAAAAATTATTCACGTGGCTGGAACTAAGGGAAAGGGTTCCACATGCACCTTTTCTGAAGCAATTTTACGGGAATGTGGTCTTCGAACTGGACTTTTCACTTCCCCTCATCTCATTGATGTGCGTGAGAGATTTCGTATAAATGGATTGGATATATCAGAAGATAAATTTCTGCATTACTTCTGGGCATGCTGGAGACAATTGACGGAAAGTGTTACAGAACATCTGCCAATGCCTGCACTATTTCAGTTTCTTACATTGTTGGCTTTTAAAATATTCATAGATGAAGAGGTTGACGTTGCAATTATTGAAGTTGGTCTTGGGGGAATGTACGATTCAACAAATGTGATAGAAGAACCTATAGTCTGTGGCATCACTTCGCTTGGGATGGACCACATGGAGAAACTCGCTAAGCCAGATTGCTTCGCACAAGGCTGGAATTCTCAAGTACCACAGCTTCCAGAAGCAATGGATGTTATCCAGAACAAGGCACGTGAGTTGATGATCCCATTAGAGGTAGCTGAACCGCTTGACCATAAAAAGCTAGATGGATTGAAGCTTGGCATGTCTGGTGATCATCAGCTTGTTAATTCTGGTCTTGCTGTTTCCCTTTGTAAATGTTGGCTTCAGAGGACTGGAAACTGGGAAAGGATGTTCCTAAATGGTTTCAAAGTGGCCAAAATACCAGATGCATTTCTTAGAGGTCTTTCAACAGCAAATCTTTCTGGGAGAGCACAGACAGTTTATGATTGTTATGCATCATCATTTAATTCTTCAACACAGTCTGAAAACTCGAATGGAGATTTAATATTCTATTTGGATGGAGCTCATAGCCCAGAAAGCATGGAGGCTTGTGCTAACTGGTTCTCTAGTGTTGTGAAGGGAAATCATAAGTCGTTACATTGTTTTAGTGCTGAAAATGTGGACAGAGATTCAGGAAATGATGATGTAAAAGTCCTGCAGGAAGCTGAGTCCACAAAGTTGTCAAAGCAGATTCTCCTATTCAATTGCATGGAAATGAGAGACCCTGAAATCTTATTTCCACGACTTGTGAATACATGTGCTTCCTCAGGTGTTCATTTTTCGAAAGCCCTTTTTGTCCCTACCATGTCAACATATAGCAAAGTCTCTTCCAGTAATTCGGTGACTTCGTCAGATATTATAAACAAGGACTTGTCGTGGCAGCTCAACCTCCAGAAACTTTGGGAGAAGATTATGCATGGGAAGGAACCAGATTCTATATCCGAAAAGGATCTTAAGCTCGACCACGAGCGAGGTTTGCCTCCTCTGTATGAGGACTTTCACTCAAGTCTGTCGGATAACAAATTCAGTGGCAGCGCAGTGATGCCTTCATTGCCATTGGCGATAAATTGGCTGAGAGATTGCGTCAGAGAATCCCCATCCATTAGACTACAGGTT

Protein sequence

MRNNLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIHVAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACWRQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGITSLGMDHMEKLAKPDCFAQGWNSQVPQLPEAMDVIQNKARELMIPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRGLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVVKGNHKSLHCFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRDPEILFPRLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDSISEKDLKLDHERGLPPLYEDFHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESPSIRLQV
BLAST of Cp4.1LG03g08420 vs. Swiss-Prot
Match: FPGS2_ARATH (Folylpolyglutamate synthase OS=Arabidopsis thaliana GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 1.7e-165
Identity = 312/547 (57.04%), Postives = 381/547 (69.65%), Query Frame = 1

Query: 6   PQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIHVA 65
           P  SSY+ A+EALS+LI++++RG   P   ++ KLE++  Y++ILDL + +K+LK+IHVA
Sbjct: 78  PALSSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVA 137

Query: 66  GTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACWRQ 125
           GTKGKGSTC FSEAILR CG RTG+FTSPHLIDVRERFRI+GLDISE+KFL YFW CW+ 
Sbjct: 138 GTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKL 197

Query: 126 LTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGITS 185
           L E   + L MP LFQFLT+LAFKIF+ E+VDVA+IEVGLGG  DSTNVI++P+VCGI S
Sbjct: 198 LKEKAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIAS 257

Query: 186 LGMDHMEKLA--------------KPDCFAQGWNSQVPQLPEAMDVIQNKARELMIPLEV 245
           LGMDHM+ L               KP   A      VPQL EAMDV+Q  A  L +PLEV
Sbjct: 258 LGMDHMDILGNTLADIAFHKAGIFKPQIPA----FTVPQLSEAMDVLQKTANNLEVPLEV 317

Query: 246 AEPLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFL 305
             PL+ KKLDG+ LG+SGDHQLVN+GLAVSL +CWLQRTGNW+++F N  K  +IP AF 
Sbjct: 318 VAPLEPKKLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFC 377

Query: 306 RGLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVV 365
           RGL+TA L GRAQ V+D  +   +SS   E   GDLIFYLDGAHSPESMEAC  WFSS V
Sbjct: 378 RGLATARLHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAV 437

Query: 366 KGNHKSLHCFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRDPEILFPRLVNT 425
           +G+       +      + G D           ++SKQILLFNCME+RDP++L P+LV T
Sbjct: 438 RGDKSLSTAVNGYMRHGEYGTD---------LNRVSKQILLFNCMEVRDPQVLLPKLVTT 497

Query: 426 CASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDS 485
           CASSG HFS+ALFVP+MSTY+KV S  S   SD   KDL+WQ  LQ+LWEK + G   D+
Sbjct: 498 CASSGTHFSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQG--TDA 557

Query: 486 ISEKDLKLDHERGLPPLYEDF-------HSSLSDNKFSGSAVMPSLPLAINWLRDCVRES 532
             +  LK D    LPP   DF           +    + SAVMPSLPL INWLRDCVR +
Sbjct: 558 GLDHTLKPDGITALPP--HDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCVRRN 607

BLAST of Cp4.1LG03g08420 vs. Swiss-Prot
Match: FPGS1_ARATH (Folylpolyglutamate synthase OS=Arabidopsis thaliana GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.3e-125
Identity = 264/533 (49.53%), Postives = 336/533 (63.04%), Query Frame = 1

Query: 10  SYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIHVAGTKG 69
           SYE AL ALSSLIT++SR   +       + E +  Y+++LDL E + K+ +IHVAGTKG
Sbjct: 66  SYEEALAALSSLITKRSRADKS---NKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKG 125

Query: 70  KGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACWRQLTES 129
           KGSTCTF+E+I+R  G RTGLFTSPHLIDVRERFR++G+DISE+KFL YFW C+ +L E 
Sbjct: 126 KGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKER 185

Query: 130 VTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGITSLGMD 189
             E +PMP  F+FL LLAFKIF  EEVD AI+EVGLGG +D+TN +++P+VCGI+SLG D
Sbjct: 186 TNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYD 245

Query: 190 HMEKLA---------KPDCFAQGWNS-QVPQLPEAMDVIQNKARELMIPLEVAEPLDHKK 249
           HME L          K   F  G  +  VPQ  EAM V++ KA E  + LEV +PL  + 
Sbjct: 246 HMEILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARL 305

Query: 250 LDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRGLSTANL 309
           L G KLG+ G+HQ VN+GLAVSL   WLQ+ G  E    +  +++ +P+ F++GL+TA+L
Sbjct: 306 LSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGKLE--VPSRTQMSILPEKFIKGLATASL 365

Query: 310 SGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVVKGNHKSLH 369
            GRAQ V D Y  S         ++GDL+FYLDGAHSPESMEACA WFS  VKG+++S  
Sbjct: 366 QGRAQVVPDQYTES--------RTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGS 425

Query: 370 CFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRDPEILFPRLVNTCASSGVHF 429
                N    S +D         S +  +QILLFNCM +RDP +L P L N CA  GV+F
Sbjct: 426 SGHLVNGSAGSSHD-------KWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNF 485

Query: 430 SKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDSISEKDLKL 489
            KALFVP MS Y KV ++  +  +D    DLSWQ  LQK+WE ++        SE+D + 
Sbjct: 486 KKALFVPNMSVYHKVGTAADLPEND-PQVDLSWQFTLQKVWESLVQ-------SERDGEK 545

Query: 490 DHERGLPPLYEDFHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESPS-IRLQV 532
           D E              SD     S V  SLP+AI  LRD V ES S  R QV
Sbjct: 546 DGE--------------SD---GNSEVFTSLPMAIKCLRDTVHESSSATRFQV 553

BLAST of Cp4.1LG03g08420 vs. Swiss-Prot
Match: FPGS3_ARATH (Folylpolyglutamate synthase OS=Arabidopsis thaliana GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.7e-112
Identity = 238/537 (44.32%), Postives = 324/537 (60.34%), Query Frame = 1

Query: 8   SSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMR---KYVEILDLVECVKKLKIIHV 67
           S+ Y++ L+ALSSLIT++ R      + S  +  R R    Y+++L+L + V ++KIIHV
Sbjct: 10  SARYQNTLDALSSLITKRGR------LASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHV 69

Query: 68  AGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACWR 127
           AGTKGKGSTCTF+E+ILR  GLRTGLFTSPHLIDVRERFR+NG++IS++KF++YFW C+ 
Sbjct: 70  AGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFH 129

Query: 128 QLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGIT 187
           +L E  +  +PMP  F FL LLAFKIF  E+VDV I+EVGLGG +D+TNVI++P+VCGI+
Sbjct: 130 KLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGIS 189

Query: 188 SLGMDHMEKLA---------KPDCFAQGWNS-QVPQLPEAMDVIQNKARELMIPLEVAEP 247
           SLG DHME L          K   F  G  +  V Q  EAM V+  KA +L + L+V EP
Sbjct: 190 SLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEP 249

Query: 248 LDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRGL 307
           LD  +    +LG+ G+HQ +N+GLAV+LC  +L+  G  ++  L+  +   +P+ F+ GL
Sbjct: 250 LDSSQ----RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLD--QTNGLPEKFISGL 309

Query: 308 STANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVVKGN 367
           S A L GRA  V D                 ++++YLDGAHSPESMEACA WFS  +K N
Sbjct: 310 SNAYLMGRAMIVPDSELPE------------EIVYYLDGAHSPESMEACAIWFSKQIKQN 369

Query: 368 HKSLHCFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRDPEILFPRLVNTCAS 427
                                    +  + K S+QILLFNCM +RDP +L PRL + C  
Sbjct: 370 -------------------------QERNQKRSEQILLFNCMSVRDPSLLLPRLRSKCID 429

Query: 428 SGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDSISE 487
            GV F +A+FVP +S Y++V SS +V +     + +SWQ  LQ++WE +  G E  S S+
Sbjct: 430 QGVDFKRAVFVPNVSVYNQVGSSTNVGTR---VESMSWQFGLQRIWESLARG-EAKSNSK 474

Query: 488 KDLKLDHERGLPPLYEDFHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESPSIRLQV 532
            D K   E                     S V  SLP+A++WLRD  R+S  +R QV
Sbjct: 490 SDSKGKEE-------------------EKSFVFSSLPVAVDWLRDNARQSKQVRFQV 474

BLAST of Cp4.1LG03g08420 vs. Swiss-Prot
Match: FOLC_CRIGR (Folylpolyglutamate synthase, mitochondrial OS=Cricetulus griseus GN=FPGS PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 8.5e-64
Identity = 182/550 (33.09%), Postives = 274/550 (49.82%), Query Frame = 1

Query: 11  YESALEALSSLITQKSRGTMAPVIRS--KTKLERMRKYVEILDL-VECVKKLKIIHVAGT 70
           Y+ A+  L++L T  S        RS  + +LE M  Y+    L VE + +L IIHV GT
Sbjct: 45  YQDAVRMLNTLQTNASYLEQVKRQRSDPQAQLEAMEVYLARSGLQVEDLNQLNIIHVTGT 104

Query: 71  KGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACWRQLT 130
           KGKGSTC F+E ILR  GL+TG F+SPHL+ VRER RING  IS + F  +FW  + QL 
Sbjct: 105 KGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGKPISPELFTKHFWRLYHQLE 164

Query: 131 ESVTE-HLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGITSL 190
           E   + H+ MPA F+FLTL+AF +F+ E+VD+A++EVG+GG YD TN+I +P+VCG++SL
Sbjct: 165 EFKDDSHVAMPAYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNIIRKPVVCGVSSL 224

Query: 191 GMDH-------MEKLA--KPDCFAQGWNSQVPQLPEA-MDVIQNKARELMIPLEVAEPLD 250
           GMDH       +EK+A  K   F  G  +     PE  + V++++A++   PL +  PL+
Sbjct: 225 GMDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVLQPEGPLAVLRDRAQQTSCPLYLCPPLE 284

Query: 251 HKKLDG--LKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAK--------- 310
             +  G  L LG+ G+HQ  N+ LA+ L  CWL++  + +   +   KV++         
Sbjct: 285 ALEDGGLPLTLGLEGEHQRSNAALALQLAHCWLEQQDHQD---IRELKVSRPSMRWQLPL 344

Query: 311 ----IPDAFLR-GLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESM 370
                P + +R GL      GR Q +                  G L +YLDGAH+  S+
Sbjct: 345 APVFHPTSHMRHGLRDTEWPGRTQVL----------------RRGPLTWYLDGAHTTSSV 404

Query: 371 EACANWFSSVVKGNHKSLHCFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRD 430
           +AC  W+            C S +     SG  +V              +LLFN    RD
Sbjct: 405 QACVRWY------------CQSLQRSKHPSGGPEV-------------YVLLFNSTGDRD 464

Query: 431 PEILFPRLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLW 490
              L  +L+  C      F  A+F P ++  S   +++    +  +++ L   L  Q+ W
Sbjct: 465 SAALL-KLLQPC-----QFDYAVFCPNLTEVSSTENADQQNFTVTLDQVLLRCLQHQQHW 524

Query: 491 EKIMHGK-EPDSISEKDLKLDHERGLPPLYEDFHSSLSDNKFSGSAVMPSLPLAINWL-- 527
             +   +  PD +S    +      L P     HS+      + S V   +  A+ W+  
Sbjct: 525 SCLSEKQASPDFLSSPSPEPGRPGSLQPALRPPHST-----GTNSLVFSCISHALQWISQ 539

BLAST of Cp4.1LG03g08420 vs. Swiss-Prot
Match: FOLC_HUMAN (Folylpolyglutamate synthase, mitochondrial OS=Homo sapiens GN=FPGS PE=1 SV=3)

HSP 1 Score: 243.0 bits (619), Expect = 7.2e-63
Identity = 171/489 (34.97%), Postives = 251/489 (51.33%), Query Frame = 1

Query: 8   SSSYESALEALSSLITQKSRGTMAPVIRSK----TKLERMRKYVEILDL-VECVKKLKII 67
           S  Y+ A+  L++L  Q + G +  V R +    T+LE M  Y+    L VE + +L II
Sbjct: 42  SMEYQDAVRMLNTL--QTNAGYLEQVKRQRGDPQTQLEAMELYLARSGLQVEDLDRLNII 101

Query: 68  HVAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWAC 127
           HV GTKGKGSTC F+E ILR  GL+TG F+SPHL+ VRER RING  IS + F  YFW  
Sbjct: 102 HVTGTKGKGSTCAFTECILRSYGLKTGFFSSPHLVQVRERIRINGQPISPELFTKYFWRL 161

Query: 128 WRQLTESVT-EHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVC 187
           + +L E+     + MP  F+FLTL+AF +F+ E+VD+A++EVG+GG YD TN+I +P+VC
Sbjct: 162 YHRLEETKDGSCVSMPPYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNIIRKPVVC 221

Query: 188 GITSLGMDH-------MEKLA--KPDCFAQGWNSQVPQLPEA-MDVIQNKARELMIPLEV 247
           G++SLG+DH       +EK+A  K   F QG  +     PE  + V++++A+++  PL +
Sbjct: 222 GVSSLGIDHTSLLGDTVEKIAWQKGGIFKQGVPAFTVLQPEGPLAVLRDRAQQISCPLYL 281

Query: 248 AEPLDHKKLDG--LKLGMSGDHQLVNSGLAVSLCKCWLQRTGN--------------WER 307
              L+  +  G  L LG+ G+HQ  N+ LA+ L  CWLQR                 W+ 
Sbjct: 282 CPMLEALEEGGPPLTLGLEGEHQRSNAALALQLAHCWLQRQDRHGAGEPKASRPGLLWQL 341

Query: 308 MFLNGFKVAKIPDAFLR-GLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGA 367
                F+    P + +R GL      GR Q +                  G L +YLDGA
Sbjct: 342 PLAPVFQ----PTSHMRLGLRNTEWPGRTQVL----------------RRGPLTWYLDGA 401

Query: 368 HSPESMEACANWFSSVVKGNHKSLHCFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFN 427
           H+  S +AC  WF   ++G             +R SG  +V+V             LLFN
Sbjct: 402 HTASSAQACVRWFRQALQGR------------ERPSGGPEVRV-------------LLFN 461

Query: 428 CMEMRDPEILFPRLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQL 464
               RDP  L  +L+  C      F  A+F P ++  S   +++    +  +++ L   L
Sbjct: 462 ATGDRDPAALL-KLLQPC-----QFDYAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRCL 477

BLAST of Cp4.1LG03g08420 vs. TrEMBL
Match: A0A0A0LHM9_CUCSA (Folylpolyglutamate synthase OS=Cucumis sativus GN=Csa_3G823100 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 5.4e-251
Identity = 456/546 (83.52%), Postives = 476/546 (87.18%), Query Frame = 1

Query: 1   MRNNLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLK 60
           MRN+LPQ  SYES +EALSSLITQK RG M   IR++ K  RMRKYVEIL + ECVKKLK
Sbjct: 1   MRNSLPQLPSYESTMEALSSLITQKRRGEMVSAIRNEEKSMRMRKYVEILGIEECVKKLK 60

Query: 61  IIHVAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFW 120
           IIHVAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFW
Sbjct: 61  IIHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFW 120

Query: 121 ACWRQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIV 180
           ACW QL E+VTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGG YDSTNVIEEPIV
Sbjct: 121 ACWTQLKENVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGTYDSTNVIEEPIV 180

Query: 181 CGITSLGMDHMEKLA--------------KPDCFAQGWNSQVPQLPEAMDVIQNKARELM 240
           CGITSLGMDHME L               KP   A      VPQLPEAMDVIQ KARELM
Sbjct: 181 CGITSLGMDHMEILGDSLGMIASHKAGILKPQVPA----FTVPQLPEAMDVIQKKARELM 240

Query: 241 IPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKI 300
           +PLEVAEPL+ KKLDGLKLGMSGDHQ VNSGLAVSLCKCWLQRTGNWERM LNG K AKI
Sbjct: 241 VPLEVAEPLNDKKLDGLKLGMSGDHQFVNSGLAVSLCKCWLQRTGNWERMLLNGCKGAKI 300

Query: 301 PDAFLRGLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANW 360
           P AFLRGLSTANLSGRAQTVYD Y SSFNS+TQS+NSNGDL+FYLDGAHSPESMEACA+W
Sbjct: 301 PAAFLRGLSTANLSGRAQTVYDYYTSSFNSTTQSDNSNGDLVFYLDGAHSPESMEACASW 360

Query: 361 FSSVVKGNHKSLHCFSAENVDRDSGNDD-VKVLQEAESTKLSKQILLFNCMEMRDPEILF 420
           FSSVVKGNHKSL+ F A+NVDRDSGNDD VKVLQEAESTKLSKQ+LLFNCME+RDP IL 
Sbjct: 361 FSSVVKGNHKSLNSFRAKNVDRDSGNDDPVKVLQEAESTKLSKQVLLFNCMEVRDPHILL 420

Query: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMH 480
           PRLVNTCASSGVHFSKALFVPTMSTYSKVSS NSVTSSD I+KDLSWQLNLQ+LWEKIMH
Sbjct: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSGNSVTSSDNISKDLSWQLNLQRLWEKIMH 480

Query: 481 GKEPDSISEKDLKLDHERGLPPLYEDFHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESP 532
           GK  D+I EKDLKLD ERGLP LYED  SSLS+N  S SAVMPSLPLAI WLRDCVRESP
Sbjct: 481 GK--DAILEKDLKLDSERGLPALYEDSLSSLSENNLSSSAVMPSLPLAIKWLRDCVRESP 540

BLAST of Cp4.1LG03g08420 vs. TrEMBL
Match: F6HLM9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g05910 PE=4 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 8.0e-178
Identity = 339/545 (62.20%), Postives = 395/545 (72.48%), Query Frame = 1

Query: 5    LPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIHV 64
            LP SSSYE A+EALSSLIT + RG  + V     KL+RM  Y++IL L E +  LKIIHV
Sbjct: 632  LPLSSSYEMAMEALSSLITSQKRGGRSSVSGKYGKLDRMSMYLKILGLEEKIAGLKIIHV 691

Query: 65   AGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACWR 124
            AGTKGKGSTCTF EAILRECG RTGLF SPHLIDVRER RI+GLDISE+KFL +FW CW 
Sbjct: 692  AGTKGKGSTCTFCEAILRECGFRTGLFISPHLIDVRERLRIDGLDISEEKFLMHFWDCWN 751

Query: 125  QLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGIT 184
            +L E VT  LPMP LFQFL++LAFKIF  EEVDVAIIEVGLGG  DSTNVI+EP+VCGIT
Sbjct: 752  RLKEKVTNDLPMPPLFQFLSVLAFKIFTCEEVDVAIIEVGLGGKRDSTNVIKEPVVCGIT 811

Query: 185  SLGMDHMEKLA--------------KPDCFAQGWNSQVPQLPEAMDVIQNKARELMIPLE 244
            SLGMDH E L               KP   A      VPQL EAMDV+Q +A EL +PLE
Sbjct: 812  SLGMDHTETLGDTLGKIASHKAGIFKPQIPA----FTVPQLSEAMDVLQQRAHELEVPLE 871

Query: 245  VAEPLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAF 304
            VA PLDHK L GLKL +SGDHQ +N+GLAVSLCKCWLQ TGNWE++  N  +   + +AF
Sbjct: 872  VAAPLDHKNLKGLKLSLSGDHQFINAGLAVSLCKCWLQSTGNWEKLLKNANQEDDLLEAF 931

Query: 305  LRGLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSV 364
            LRGLSTA LSGRAQTVYD    S+N S  +ENS GDLIFYLDGAHSPESM+ CA WFS+ 
Sbjct: 932  LRGLSTARLSGRAQTVYDTPLKSYNLSEATENSFGDLIFYLDGAHSPESMDVCARWFSNA 991

Query: 365  VKGNHKSLHCFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRDPEILFPRLVN 424
            VK    S      +  +  +G    K     ES K+SKQILLFNCME+RDP IL P+LV 
Sbjct: 992  VKERRNSPSSSFVKVGNMVNGYIHHKKEDTEESNKISKQILLFNCMEVRDPHILLPQLVR 1051

Query: 425  TCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPD 484
            TCASSG HFSKALFVP++STY+KV+S +S+   D+  +DLSWQ NLQ++WEKI+H K  D
Sbjct: 1052 TCASSGTHFSKALFVPSISTYNKVTSGDSIVPLDLPARDLSWQFNLQRIWEKIIHNK--D 1111

Query: 485  SISEKDLKLDHERGLPP---LYEDFHSSLSDNK-FSGSAVMPSLPLAINWLRDCVRESPS 532
             + ++  K+D  R LPP   LYE+  S    +K  S SAVMPSLPL I WLRDCV+E+PS
Sbjct: 1112 VVVDESFKMDSPRILPPFEFLYENGSSGSPSSKCLSSSAVMPSLPLTIRWLRDCVKENPS 1170

BLAST of Cp4.1LG03g08420 vs. TrEMBL
Match: B9IHV7_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0016s04130g PE=4 SV=2)

HSP 1 Score: 629.0 bits (1621), Expect = 5.2e-177
Identity = 339/549 (61.75%), Postives = 408/549 (74.32%), Query Frame = 1

Query: 4   NLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIH 63
           ++P S SYE+ALEALSSLI Q+ RG    +     KL+RMR Y++ILDL E V  LKIIH
Sbjct: 82  DVPLSDSYEAALEALSSLIRQQKRGDQKTIGGKYGKLDRMRMYLKILDLEERVAGLKIIH 141

Query: 64  VAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACW 123
           VAGTKGKGSTCTF EAILRE G RTGLFTSPHLIDVRERFRING+DISE KFL YFW CW
Sbjct: 142 VAGTKGKGSTCTFCEAILRESGFRTGLFTSPHLIDVRERFRINGVDISEVKFLLYFWNCW 201

Query: 124 RQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGI 183
            QL E  TE LPMP LFQFLT+LAFKIF+ E+VDV+IIEVGLGG  DSTNVIE+P+VCGI
Sbjct: 202 NQLKEHETEDLPMPPLFQFLTVLAFKIFVCEQVDVSIIEVGLGGRNDSTNVIEKPVVCGI 261

Query: 184 TSLGMDHMEKLAK-----PDCFAQGWNSQVP-----QLPEAMDVIQNKARELMIPLEVAE 243
           TSLGMDH E L           A  +  Q+P     Q+ EAMDV+Q  ARELM+PL+V E
Sbjct: 262 TSLGMDHTETLGNTIGQIASHKAGVFKHQIPAFTVLQVSEAMDVLQENARELMVPLKVVE 321

Query: 244 PLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRG 303
           PLD K L+GLKL +SGDHQ  N+GLAVSLCK WLQRTGNWE++F    + A +P+AFLRG
Sbjct: 322 PLDSKALNGLKLSLSGDHQFSNAGLAVSLCKSWLQRTGNWEKLFQKDNREANLPEAFLRG 381

Query: 304 LSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVVKG 363
           LSTA++SGRAQ V+D  +SS + S++   ++GDLIFYLDGAHSPES+EACA WFS  VKG
Sbjct: 382 LSTAHISGRAQIVHDSSSSSSHVSSEVAETSGDLIFYLDGAHSPESIEACAKWFSVAVKG 441

Query: 364 NHK-------SLHCFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRDPEILFP 423
           N++       S H     N+ R +G+   +     E  K+SK+ILLFNCME+RDP+IL P
Sbjct: 442 NNQSPPLVLSSSHNIENINIVRKNGHVQHEKCNIQEFNKISKKILLFNCMEVRDPQILLP 501

Query: 424 RLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHG 483
           RLV+TCASSG  FSKA+FVP++STY+KV+S  SV  SDI +KDLSWQ +LQ+LWE+I+HG
Sbjct: 502 RLVSTCASSGTFFSKAIFVPSISTYNKVTSGTSVIPSDISSKDLSWQFSLQRLWERIVHG 561

Query: 484 KEPDSISEKDLKLDHERGLPP---LYED-FHSSLSDNKFSGSAVMPSLPLAINWLRDCVR 532
            + D + EK  K+D     P    LYED  + S S+   + SAV+PSLPL I WLRDCVR
Sbjct: 562 IDTD-LLEKSTKMDAAETSPHREFLYEDASNCSPSNGYLACSAVIPSLPLTIKWLRDCVR 621

BLAST of Cp4.1LG03g08420 vs. TrEMBL
Match: A0A061F3L1_THECC (Dhfs-fpgs c isoform 1 OS=Theobroma cacao GN=TCM_024488 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.3e-175
Identity = 331/545 (60.73%), Postives = 404/545 (74.13%), Query Frame = 1

Query: 4   NLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIH 63
           ++P S SYE A++ALSSLIT++ RG  + V+    KL+RM  Y++IL L E V  LKIIH
Sbjct: 95  DVPLSFSYEPAMDALSSLITRQKRGERSNVMGKYGKLDRMLMYLKILGLEEQVAGLKIIH 154

Query: 64  VAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACW 123
           VAGTKGKGSTCTF EAILRE G RTGLFTSPHLIDVRERFR++GL+ISE+KFL YFW CW
Sbjct: 155 VAGTKGKGSTCTFCEAILRESGFRTGLFTSPHLIDVRERFRLDGLEISEEKFLQYFWNCW 214

Query: 124 RQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGI 183
             L E++T+ LPMP LFQFLT+LAFKIF+ E+VDVAIIEVGLGG  DSTNVI++P+VCGI
Sbjct: 215 HILKENITDALPMPPLFQFLTVLAFKIFVCEQVDVAIIEVGLGGTRDSTNVIKQPVVCGI 274

Query: 184 TSLGMDHMEKLA---------KPDCFAQGWNS-QVPQLPEAMDVIQNKARELMIPLEVAE 243
           TSLGMDHME L          K   F     +  VPQL EAM+V+Q +A ELM+PL VA 
Sbjct: 275 TSLGMDHMETLGNTLGQIALHKAGIFKHQIPAFTVPQLSEAMEVLQERAHELMVPLNVAA 334

Query: 244 PLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRG 303
           PLD K L G+K+ +SGDHQL+N+GLAVSLCKCWLQRTGNWE++  N  +   IP+AF RG
Sbjct: 335 PLDSKNLKGVKISLSGDHQLINAGLAVSLCKCWLQRTGNWEKILHNDSQETDIPEAFCRG 394

Query: 304 LSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVVKG 363
           LSTA LSGRA+ VYD ++  +NSS   ENS G+LIFYLDGAHSPESMEACA WFSSV KG
Sbjct: 395 LSTACLSGRAEIVYDKFSKYYNSSATDENSCGNLIFYLDGAHSPESMEACARWFSSVAKG 454

Query: 364 NHK-SLHCFSAENVDRDSGNDDVKVLQE--AESTKLSKQILLFNCMEMRDPEILFPRLVN 423
           N K S    SA    R+ GN   + +++   ES  +SKQILLFNCME+RDP++L   LVN
Sbjct: 455 NGKVSPLSLSAL---RNFGNGCARHIKDKVEESHVISKQILLFNCMEVRDPQVLLTHLVN 514

Query: 424 TCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPD 483
            C+SSG H SKALFVP MS Y+KV+S  S+T+SD  + D SWQ +LQ++WEK++HG  PD
Sbjct: 515 ACSSSGTHLSKALFVPNMSKYNKVTSGTSITASDSTSGDFSWQFSLQRIWEKVIHG--PD 574

Query: 484 SISEKDLKLDHERGLPP---LYED-FHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESPS 532
            + ++   ++    LP    LYED FH S +D   + SAVMPSLP  I WLRDCV+E PS
Sbjct: 575 PVLDRSTMMNRTVSLPSLEFLYEDAFHCSAADQHLACSAVMPSLPQTIKWLRDCVKEKPS 634

BLAST of Cp4.1LG03g08420 vs. TrEMBL
Match: B9H9H1_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s04390g PE=4 SV=2)

HSP 1 Score: 623.6 bits (1607), Expect = 2.2e-175
Identity = 338/550 (61.45%), Postives = 412/550 (74.91%), Query Frame = 1

Query: 4   NLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIH 63
           ++P S+SYE A+EALSSLI QK RG    +     KL+RMR Y++ILDL E +  LKIIH
Sbjct: 87  DVPLSTSYEVAMEALSSLIRQKKRGDRKGIGGKYGKLDRMRIYLKILDLEEHMAGLKIIH 146

Query: 64  VAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACW 123
           VAGTKGKGSTCTF EA+LRE G RTGLFTSPHLIDVRERFRING+DISEDKFL YFW CW
Sbjct: 147 VAGTKGKGSTCTFCEAMLRESGFRTGLFTSPHLIDVRERFRINGVDISEDKFLSYFWNCW 206

Query: 124 RQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGI 183
            +L E  TE LPMP LFQFLT+LAFKIF+ E+VDV+IIEVGLGG  DSTNVIEEP+VCGI
Sbjct: 207 NRLKEHETEDLPMPPLFQFLTVLAFKIFVCEQVDVSIIEVGLGGRNDSTNVIEEPVVCGI 266

Query: 184 TSLGMDHMEKLA---------KPDCFAQGWNS-QVPQLPEAMDVIQNKARELMIPLEVAE 243
           TSLGMDH E L          K   F     +  VPQ+ EAMDV+Q  A+EL +PL+V E
Sbjct: 267 TSLGMDHTEALGNTIGQIASHKAGIFKHQIPAFTVPQVSEAMDVLQENAQELTVPLKVVE 326

Query: 244 PLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRG 303
           PLD  +L+GLKL +SG+HQL N+GLAVSLCKCWLQRTGNWE++F    K A +P+AFLRG
Sbjct: 327 PLDSNELNGLKLSLSGNHQLSNAGLAVSLCKCWLQRTGNWEKLFQIDNKEANLPEAFLRG 386

Query: 304 LSTANLSGRAQTVYDCYASSFNS-STQSENSNGDLIFYLDGAHSPESMEACANWFSSVVK 363
           LS A+++GRAQ V D  ++S +  S++   ++GDLIFYLDGAHSPESME CA WFSS VK
Sbjct: 387 LSKAHIAGRAQIVPDLSSTSSSCISSEVAEASGDLIFYLDGAHSPESMEVCAEWFSSAVK 446

Query: 364 GNH--KSLHCFSA---ENVDRDSGNDDV--KVLQEAESTKLSKQILLFNCMEMRDPEILF 423
            N+   SL  FS+   E++++  GN  +  +     E  K+SK+ILLFNCM++RDP+IL 
Sbjct: 447 ENNLSSSLVSFSSHDIESINKVPGNGYMQHEKFNIQEINKISKKILLFNCMDVRDPQILL 506

Query: 424 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMH 483
           PRLV+TCASSG  FSKA+FVP++STY+KV+S  SV  SDI +KDLSWQ +LQ+LWEKI+H
Sbjct: 507 PRLVSTCASSGTFFSKAIFVPSISTYNKVTSGTSVVPSDISSKDLSWQFSLQRLWEKIVH 566

Query: 484 GKEPDSISEKDLKLDHERGLPP---LYED-FHSSLSDNKFSGSAVMPSLPLAINWLRDCV 532
           G + DS+ EK  K+D    LP    LYED  + S +D   + SAV+PSLPL I WLR CV
Sbjct: 567 GIDTDSLLEKSTKMDGAETLPRRQFLYEDASNCSPTDGYLACSAVIPSLPLTIKWLRHCV 626

BLAST of Cp4.1LG03g08420 vs. TAIR10
Match: AT3G10160.1 (AT3G10160.1 DHFS-FPGS homolog C)

HSP 1 Score: 583.9 bits (1504), Expect = 9.7e-167
Identity = 312/547 (57.04%), Postives = 381/547 (69.65%), Query Frame = 1

Query: 6   PQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIHVA 65
           P  SSY+ A+EALS+LI++++RG   P   ++ KLE++  Y++ILDL + +K+LK+IHVA
Sbjct: 78  PALSSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVA 137

Query: 66  GTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACWRQ 125
           GTKGKGSTC FSEAILR CG RTG+FTSPHLIDVRERFRI+GLDISE+KFL YFW CW+ 
Sbjct: 138 GTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKL 197

Query: 126 LTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGITS 185
           L E   + L MP LFQFLT+LAFKIF+ E+VDVA+IEVGLGG  DSTNVI++P+VCGI S
Sbjct: 198 LKEKAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIAS 257

Query: 186 LGMDHMEKLA--------------KPDCFAQGWNSQVPQLPEAMDVIQNKARELMIPLEV 245
           LGMDHM+ L               KP   A      VPQL EAMDV+Q  A  L +PLEV
Sbjct: 258 LGMDHMDILGNTLADIAFHKAGIFKPQIPA----FTVPQLSEAMDVLQKTANNLEVPLEV 317

Query: 246 AEPLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFL 305
             PL+ KKLDG+ LG+SGDHQLVN+GLAVSL +CWLQRTGNW+++F N  K  +IP AF 
Sbjct: 318 VAPLEPKKLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFC 377

Query: 306 RGLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVV 365
           RGL+TA L GRAQ V+D  +   +SS   E   GDLIFYLDGAHSPESMEAC  WFSS V
Sbjct: 378 RGLATARLHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAV 437

Query: 366 KGNHKSLHCFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRDPEILFPRLVNT 425
           +G+       +      + G D           ++SKQILLFNCME+RDP++L P+LV T
Sbjct: 438 RGDKSLSTAVNGYMRHGEYGTD---------LNRVSKQILLFNCMEVRDPQVLLPKLVTT 497

Query: 426 CASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDS 485
           CASSG HFS+ALFVP+MSTY+KV S  S   SD   KDL+WQ  LQ+LWEK + G   D+
Sbjct: 498 CASSGTHFSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQG--TDA 557

Query: 486 ISEKDLKLDHERGLPPLYEDF-------HSSLSDNKFSGSAVMPSLPLAINWLRDCVRES 532
             +  LK D    LPP   DF           +    + SAVMPSLPL INWLRDCVR +
Sbjct: 558 GLDHTLKPDGITALPP--HDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCVRRN 607

BLAST of Cp4.1LG03g08420 vs. TAIR10
Match: AT5G05980.1 (AT5G05980.1 DHFS-FPGS homolog B)

HSP 1 Score: 451.4 bits (1160), Expect = 7.5e-127
Identity = 264/533 (49.53%), Postives = 336/533 (63.04%), Query Frame = 1

Query: 10  SYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIHVAGTKG 69
           SYE AL ALSSLIT++SR   +       + E +  Y+++LDL E + K+ +IHVAGTKG
Sbjct: 66  SYEEALAALSSLITKRSRADKS---NKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKG 125

Query: 70  KGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACWRQLTES 129
           KGSTCTF+E+I+R  G RTGLFTSPHLIDVRERFR++G+DISE+KFL YFW C+ +L E 
Sbjct: 126 KGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKER 185

Query: 130 VTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGITSLGMD 189
             E +PMP  F+FL LLAFKIF  EEVD AI+EVGLGG +D+TN +++P+VCGI+SLG D
Sbjct: 186 TNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYD 245

Query: 190 HMEKLA---------KPDCFAQGWNS-QVPQLPEAMDVIQNKARELMIPLEVAEPLDHKK 249
           HME L          K   F  G  +  VPQ  EAM V++ KA E  + LEV +PL  + 
Sbjct: 246 HMEILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARL 305

Query: 250 LDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRGLSTANL 309
           L G KLG+ G+HQ VN+GLAVSL   WLQ+ G  E    +  +++ +P+ F++GL+TA+L
Sbjct: 306 LSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGKLE--VPSRTQMSILPEKFIKGLATASL 365

Query: 310 SGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVVKGNHKSLH 369
            GRAQ V D Y  S         ++GDL+FYLDGAHSPESMEACA WFS  VKG+++S  
Sbjct: 366 QGRAQVVPDQYTES--------RTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGS 425

Query: 370 CFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRDPEILFPRLVNTCASSGVHF 429
                N    S +D         S +  +QILLFNCM +RDP +L P L N CA  GV+F
Sbjct: 426 SGHLVNGSAGSSHD-------KWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNF 485

Query: 430 SKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDSISEKDLKL 489
            KALFVP MS Y KV ++  +  +D    DLSWQ  LQK+WE ++        SE+D + 
Sbjct: 486 KKALFVPNMSVYHKVGTAADLPEND-PQVDLSWQFTLQKVWESLVQ-------SERDGEK 545

Query: 490 DHERGLPPLYEDFHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESPS-IRLQV 532
           D E              SD     S V  SLP+AI  LRD V ES S  R QV
Sbjct: 546 DGE--------------SD---GNSEVFTSLPMAIKCLRDTVHESSSATRFQV 553

BLAST of Cp4.1LG03g08420 vs. TAIR10
Match: AT3G55630.3 (AT3G55630.3 DHFS-FPGS homolog D)

HSP 1 Score: 407.9 bits (1047), Expect = 9.5e-114
Identity = 238/537 (44.32%), Postives = 324/537 (60.34%), Query Frame = 1

Query: 8   SSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMR---KYVEILDLVECVKKLKIIHV 67
           S+ Y++ L+ALSSLIT++ R      + S  +  R R    Y+++L+L + V ++KIIHV
Sbjct: 10  SARYQNTLDALSSLITKRGR------LASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHV 69

Query: 68  AGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACWR 127
           AGTKGKGSTCTF+E+ILR  GLRTGLFTSPHLIDVRERFR+NG++IS++KF++YFW C+ 
Sbjct: 70  AGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFH 129

Query: 128 QLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGIT 187
           +L E  +  +PMP  F FL LLAFKIF  E+VDV I+EVGLGG +D+TNVI++P+VCGI+
Sbjct: 130 KLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGIS 189

Query: 188 SLGMDHMEKLA---------KPDCFAQGWNS-QVPQLPEAMDVIQNKARELMIPLEVAEP 247
           SLG DHME L          K   F  G  +  V Q  EAM V+  KA +L + L+V EP
Sbjct: 190 SLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEP 249

Query: 248 LDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRGL 307
           LD  +    +LG+ G+HQ +N+GLAV+LC  +L+  G  ++  L+  +   +P+ F+ GL
Sbjct: 250 LDSSQ----RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLD--QTNGLPEKFISGL 309

Query: 308 STANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVVKGN 367
           S A L GRA  V D                 ++++YLDGAHSPESMEACA WFS  +K N
Sbjct: 310 SNAYLMGRAMIVPDSELPE------------EIVYYLDGAHSPESMEACAIWFSKQIKQN 369

Query: 368 HKSLHCFSAENVDRDSGNDDVKVLQEAESTKLSKQILLFNCMEMRDPEILFPRLVNTCAS 427
                                    +  + K S+QILLFNCM +RDP +L PRL + C  
Sbjct: 370 -------------------------QERNQKRSEQILLFNCMSVRDPSLLLPRLRSKCID 429

Query: 428 SGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDSISE 487
            GV F +A+FVP +S Y++V SS +V +     + +SWQ  LQ++WE +  G E  S S+
Sbjct: 430 QGVDFKRAVFVPNVSVYNQVGSSTNVGTR---VESMSWQFGLQRIWESLARG-EAKSNSK 474

Query: 488 KDLKLDHERGLPPLYEDFHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESPSIRLQV 532
            D K   E                     S V  SLP+A++WLRD  R+S  +R QV
Sbjct: 490 SDSKGKEE-------------------EKSFVFSSLPVAVDWLRDNARQSKQVRFQV 474

BLAST of Cp4.1LG03g08420 vs. TAIR10
Match: AT5G41480.1 (AT5G41480.1 Folylpolyglutamate synthetase family protein)

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-24
Identity = 59/139 (42.45%), Postives = 83/139 (59.71%), Query Frame = 1

Query: 58  KLKIIHVAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLH 117
           K K++HVAGTKGKGST  F   ILR  G   G ++SPH++ ++ER   NG  +S      
Sbjct: 93  KYKVVHVAGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISCNGEPVSASTLND 152

Query: 118 YFWACWRQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEE 177
            F++    L +S+ E     + F+ LT +AF +F  E VD+A+IE GLGG  D+TNVIE 
Sbjct: 153 LFYSVKPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIES 212

Query: 178 PIVCG--ITSLGMDHMEKL 195
             +    IT++G +HM  L
Sbjct: 213 SNLAASVITTIGEEHMAAL 231

BLAST of Cp4.1LG03g08420 vs. NCBI nr
Match: gi|449437789|ref|XP_004136673.1| (PREDICTED: folylpolyglutamate synthase isoform X1 [Cucumis sativus])

HSP 1 Score: 874.8 bits (2259), Expect = 7.8e-251
Identity = 456/546 (83.52%), Postives = 476/546 (87.18%), Query Frame = 1

Query: 1   MRNNLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLK 60
           MRN+LPQ  SYES +EALSSLITQK RG M   IR++ K  RMRKYVEIL + ECVKKLK
Sbjct: 1   MRNSLPQLPSYESTMEALSSLITQKRRGEMVSAIRNEEKSMRMRKYVEILGIEECVKKLK 60

Query: 61  IIHVAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFW 120
           IIHVAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFW
Sbjct: 61  IIHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFW 120

Query: 121 ACWRQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIV 180
           ACW QL E+VTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGG YDSTNVIEEPIV
Sbjct: 121 ACWTQLKENVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGTYDSTNVIEEPIV 180

Query: 181 CGITSLGMDHMEKLA--------------KPDCFAQGWNSQVPQLPEAMDVIQNKARELM 240
           CGITSLGMDHME L               KP   A      VPQLPEAMDVIQ KARELM
Sbjct: 181 CGITSLGMDHMEILGDSLGMIASHKAGILKPQVPA----FTVPQLPEAMDVIQKKARELM 240

Query: 241 IPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKI 300
           +PLEVAEPL+ KKLDGLKLGMSGDHQ VNSGLAVSLCKCWLQRTGNWERM LNG K AKI
Sbjct: 241 VPLEVAEPLNDKKLDGLKLGMSGDHQFVNSGLAVSLCKCWLQRTGNWERMLLNGCKGAKI 300

Query: 301 PDAFLRGLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANW 360
           P AFLRGLSTANLSGRAQTVYD Y SSFNS+TQS+NSNGDL+FYLDGAHSPESMEACA+W
Sbjct: 301 PAAFLRGLSTANLSGRAQTVYDYYTSSFNSTTQSDNSNGDLVFYLDGAHSPESMEACASW 360

Query: 361 FSSVVKGNHKSLHCFSAENVDRDSGNDD-VKVLQEAESTKLSKQILLFNCMEMRDPEILF 420
           FSSVVKGNHKSL+ F A+NVDRDSGNDD VKVLQEAESTKLSKQ+LLFNCME+RDP IL 
Sbjct: 361 FSSVVKGNHKSLNSFRAKNVDRDSGNDDPVKVLQEAESTKLSKQVLLFNCMEVRDPHILL 420

Query: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMH 480
           PRLVNTCASSGVHFSKALFVPTMSTYSKVSS NSVTSSD I+KDLSWQLNLQ+LWEKIMH
Sbjct: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSGNSVTSSDNISKDLSWQLNLQRLWEKIMH 480

Query: 481 GKEPDSISEKDLKLDHERGLPPLYEDFHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESP 532
           GK  D+I EKDLKLD ERGLP LYED  SSLS+N  S SAVMPSLPLAI WLRDCVRESP
Sbjct: 481 GK--DAILEKDLKLDSERGLPALYEDSLSSLSENNLSSSAVMPSLPLAIKWLRDCVRESP 540

BLAST of Cp4.1LG03g08420 vs. NCBI nr
Match: gi|659085292|ref|XP_008443342.1| (PREDICTED: folylpolyglutamate synthase isoform X1 [Cucumis melo])

HSP 1 Score: 867.5 bits (2240), Expect = 1.2e-248
Identity = 450/546 (82.42%), Postives = 473/546 (86.63%), Query Frame = 1

Query: 1   MRNNLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLK 60
           MRN+LPQ  SYESA+EALSSLI QK RG M   I  + KL +MRKY+EIL + ECVKKLK
Sbjct: 1   MRNSLPQLPSYESAMEALSSLIRQKRRGEMVSAISKEEKLVKMRKYIEILGVGECVKKLK 60

Query: 61  IIHVAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFW 120
           IIHVAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFW
Sbjct: 61  IIHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFW 120

Query: 121 ACWRQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIV 180
           ACW QL E+VTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGG YDSTNVIEEPIV
Sbjct: 121 ACWTQLKENVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGTYDSTNVIEEPIV 180

Query: 181 CGITSLGMDHMEKLA--------------KPDCFAQGWNSQVPQLPEAMDVIQNKARELM 240
           CGITSLGMDHME L               KP   A      VPQLPEAMDVIQ KARELM
Sbjct: 181 CGITSLGMDHMEILGDSLGMIASHKAGILKPQVPA----FTVPQLPEAMDVIQKKARELM 240

Query: 241 IPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKI 300
           +PLEVAEPLD KKLDGLKLGMSGDHQ VNSGLAVSLCKCWLQRTGNWERMFLNGFK AKI
Sbjct: 241 VPLEVAEPLDVKKLDGLKLGMSGDHQFVNSGLAVSLCKCWLQRTGNWERMFLNGFKGAKI 300

Query: 301 PDAFLRGLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANW 360
           P AFLRGLSTANLSGRAQ VYDCY  SF+S+TQS+NSNGDL+FYLDGAHSPESMEACA+W
Sbjct: 301 PAAFLRGLSTANLSGRAQIVYDCYTPSFHSTTQSDNSNGDLVFYLDGAHSPESMEACASW 360

Query: 361 FSSVVKGNHKSLHCFSAENVDRDSGNDD-VKVLQEAESTKLSKQILLFNCMEMRDPEILF 420
           FSSVVKGNHKSL+CF A+ +DRDSGNDD V+V QEAESTKLSKQILLFNCME+RDP IL 
Sbjct: 361 FSSVVKGNHKSLNCFRAKTMDRDSGNDDPVEVPQEAESTKLSKQILLFNCMEVRDPHILL 420

Query: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMH 480
           PRLVNTCASSGVHFSKALFVPTMSTYSKVSS NSVT SD I+KDLSWQLNLQ+LWEKIMH
Sbjct: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSGNSVTPSDNISKDLSWQLNLQRLWEKIMH 480

Query: 481 GKEPDSISEKDLKLDHERGLPPLYEDFHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESP 532
           GK  D+I EKDLKLD ERGLP LYED  SSLS+N  S SA +PSLPLAI WLRDCVRESP
Sbjct: 481 GK--DAILEKDLKLDSERGLPALYEDSLSSLSENNLSSSAAVPSLPLAIKWLRDCVRESP 540

BLAST of Cp4.1LG03g08420 vs. NCBI nr
Match: gi|778685402|ref|XP_011652214.1| (PREDICTED: folylpolyglutamate synthase isoform X2 [Cucumis sativus])

HSP 1 Score: 781.9 bits (2018), Expect = 6.8e-223
Identity = 405/483 (83.85%), Postives = 424/483 (87.78%), Query Frame = 1

Query: 1   MRNNLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLK 60
           MRN+LPQ  SYES +EALSSLITQK RG M   IR++ K  RMRKYVEIL + ECVKKLK
Sbjct: 1   MRNSLPQLPSYESTMEALSSLITQKRRGEMVSAIRNEEKSMRMRKYVEILGIEECVKKLK 60

Query: 61  IIHVAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFW 120
           IIHVAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFW
Sbjct: 61  IIHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFW 120

Query: 121 ACWRQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIV 180
           ACW QL E+VTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGG YDSTNVIEEPIV
Sbjct: 121 ACWTQLKENVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGTYDSTNVIEEPIV 180

Query: 181 CGITSLGMDHMEKLA--------------KPDCFAQGWNSQVPQLPEAMDVIQNKARELM 240
           CGITSLGMDHME L               KP   A      VPQLPEAMDVIQ KARELM
Sbjct: 181 CGITSLGMDHMEILGDSLGMIASHKAGILKPQVPA----FTVPQLPEAMDVIQKKARELM 240

Query: 241 IPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKI 300
           +PLEVAEPL+ KKLDGLKLGMSGDHQ VNSGLAVSLCKCWLQRTGNWERM LNG K AKI
Sbjct: 241 VPLEVAEPLNDKKLDGLKLGMSGDHQFVNSGLAVSLCKCWLQRTGNWERMLLNGCKGAKI 300

Query: 301 PDAFLRGLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANW 360
           P AFLRGLSTANLSGRAQTVYD Y SSFNS+TQS+NSNGDL+FYLDGAHSPESMEACA+W
Sbjct: 301 PAAFLRGLSTANLSGRAQTVYDYYTSSFNSTTQSDNSNGDLVFYLDGAHSPESMEACASW 360

Query: 361 FSSVVKGNHKSLHCFSAENVDRDSGNDD-VKVLQEAESTKLSKQILLFNCMEMRDPEILF 420
           FSSVVKGNHKSL+ F A+NVDRDSGNDD VKVLQEAESTKLSKQ+LLFNCME+RDP IL 
Sbjct: 361 FSSVVKGNHKSLNSFRAKNVDRDSGNDDPVKVLQEAESTKLSKQVLLFNCMEVRDPHILL 420

Query: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMH 469
           PRLVNTCASSGVHFSKALFVPTMSTYSKVSS NSVTSSD I+KDLSWQLNLQ+LWEKIMH
Sbjct: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSGNSVTSSDNISKDLSWQLNLQRLWEKIMH 479

BLAST of Cp4.1LG03g08420 vs. NCBI nr
Match: gi|659085294|ref|XP_008443343.1| (PREDICTED: folylpolyglutamate synthase isoform X2 [Cucumis melo])

HSP 1 Score: 777.7 bits (2007), Expect = 1.3e-221
Identity = 401/483 (83.02%), Postives = 422/483 (87.37%), Query Frame = 1

Query: 1   MRNNLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLK 60
           MRN+LPQ  SYESA+EALSSLI QK RG M   I  + KL +MRKY+EIL + ECVKKLK
Sbjct: 1   MRNSLPQLPSYESAMEALSSLIRQKRRGEMVSAISKEEKLVKMRKYIEILGVGECVKKLK 60

Query: 61  IIHVAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFW 120
           IIHVAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFW
Sbjct: 61  IIHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFW 120

Query: 121 ACWRQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIV 180
           ACW QL E+VTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGG YDSTNVIEEPIV
Sbjct: 121 ACWTQLKENVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGTYDSTNVIEEPIV 180

Query: 181 CGITSLGMDHMEKLA--------------KPDCFAQGWNSQVPQLPEAMDVIQNKARELM 240
           CGITSLGMDHME L               KP   A      VPQLPEAMDVIQ KARELM
Sbjct: 181 CGITSLGMDHMEILGDSLGMIASHKAGILKPQVPA----FTVPQLPEAMDVIQKKARELM 240

Query: 241 IPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKI 300
           +PLEVAEPLD KKLDGLKLGMSGDHQ VNSGLAVSLCKCWLQRTGNWERMFLNGFK AKI
Sbjct: 241 VPLEVAEPLDVKKLDGLKLGMSGDHQFVNSGLAVSLCKCWLQRTGNWERMFLNGFKGAKI 300

Query: 301 PDAFLRGLSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANW 360
           P AFLRGLSTANLSGRAQ VYDCY  SF+S+TQS+NSNGDL+FYLDGAHSPESMEACA+W
Sbjct: 301 PAAFLRGLSTANLSGRAQIVYDCYTPSFHSTTQSDNSNGDLVFYLDGAHSPESMEACASW 360

Query: 361 FSSVVKGNHKSLHCFSAENVDRDSGNDD-VKVLQEAESTKLSKQILLFNCMEMRDPEILF 420
           FSSVVKGNHKSL+CF A+ +DRDSGNDD V+V QEAESTKLSKQILLFNCME+RDP IL 
Sbjct: 361 FSSVVKGNHKSLNCFRAKTMDRDSGNDDPVEVPQEAESTKLSKQILLFNCMEVRDPHILL 420

Query: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMH 469
           PRLVNTCASSGVHFSKALFVPTMSTYSKVSS NSVT SD I+KDLSWQLNLQ+LWEKIMH
Sbjct: 421 PRLVNTCASSGVHFSKALFVPTMSTYSKVSSGNSVTPSDNISKDLSWQLNLQRLWEKIMH 479

BLAST of Cp4.1LG03g08420 vs. NCBI nr
Match: gi|1009159846|ref|XP_015898037.1| (PREDICTED: folylpolyglutamate synthase [Ziziphus jujuba])

HSP 1 Score: 652.5 bits (1682), Expect = 6.3e-184
Identity = 338/543 (62.25%), Postives = 418/543 (76.98%), Query Frame = 1

Query: 4   NLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIH 63
           + P +SSYESA+EALSSLIT++ RG  + +     KLERM  Y+++LDL E ++ LKIIH
Sbjct: 76  DFPFASSYESAMEALSSLITRQKRGGRSSIGGKYGKLERMSMYLKMLDLEEHIEGLKIIH 135

Query: 64  VAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACW 123
           VAGTKGKGSTCTF EAILRECGLRTGLFTSPHL+DVRERFR++GL+I+E+KFL YFW CW
Sbjct: 136 VAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLVDVRERFRLDGLEITENKFLQYFWECW 195

Query: 124 RQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGI 183
           R+L +++TE LPMP LFQFLT+LAFKIFI E+VDVAIIEVGLGG  DSTNVI EP+VCGI
Sbjct: 196 RKLEDNITEDLPMPPLFQFLTVLAFKIFISEQVDVAIIEVGLGGKNDSTNVINEPVVCGI 255

Query: 184 TSLGMDHMEKLAKPDCFAQGWNSQV--PQLP--------EAMDVIQNKARELMIPLEVAE 243
           TSLGMDH + L           + +  P++P        EAMDVIQ  A +LM+PLEVA+
Sbjct: 256 TSLGMDHTDVLGNTLGQIASHKAGIFKPKIPAFTVSQSLEAMDVIQEIACQLMVPLEVAQ 315

Query: 244 PLDHKKLDGLKLGMSGDHQLVNSGLAVSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRG 303
           P+DHKKL GL+L ++GDHQ VN+GLAVSLCKCWLQRTGNWE++  N  + A +PDAFLRG
Sbjct: 316 PVDHKKLKGLELSLAGDHQFVNAGLAVSLCKCWLQRTGNWEKLLKNDIQEADLPDAFLRG 375

Query: 304 LSTANLSGRAQTVYDCYASSFNSSTQSENSNGDLIFYLDGAHSPESMEACANWFSSVVKG 363
           LSTA +SGRAQ V+D Y +S++SS  S +S GDLIFYLDGAHSPESME C  WFSS  KG
Sbjct: 376 LSTAYISGRAQIVHDTYPTSYSSSHVSGSSCGDLIFYLDGAHSPESMEVCGRWFSSASKG 435

Query: 364 NHKSLHCFSAENVDRDSGNDDVKVLQE--AESTKLSKQILLFNCMEMRDPEILFPRLVNT 423
           N       S+E+ +   GN  ++  +E    S K+SKQILLFNCM++RDP+IL PRLV+T
Sbjct: 436 NKN-----SSESEEGIWGNGHLQHKRENMESSCKMSKQILLFNCMDVRDPQILLPRLVST 495

Query: 424 CASSGVHFSKALFVPTMSTYSKVSSSNSVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDS 483
           CASSG HFSKALFVP++S Y+KV+S  SV   D  N+DLSWQ +LQ+LWE+I+HG++ D+
Sbjct: 496 CASSGTHFSKALFVPSISAYNKVTSGASVIPLDNFNRDLSWQFHLQRLWERILHGRDVDT 555

Query: 484 ISEKDLKLDHERGLPP--LYED-FHSSLSDNKFSGSAVMPSLPLAINWLRDCVRESPSIR 532
             +   K+DH + +P   LYED  H S +DN F+ SAVMPSLPL I WLRDCV+E+PS+R
Sbjct: 556 AFDNGSKMDHAKIIPHEFLYEDATHCSPTDNHFACSAVMPSLPLTIKWLRDCVKENPSLR 613

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
FPGS2_ARATH1.7e-16557.04Folylpolyglutamate synthase OS=Arabidopsis thaliana GN=FPGS2 PE=1 SV=1[more]
FPGS1_ARATH1.3e-12549.53Folylpolyglutamate synthase OS=Arabidopsis thaliana GN=FPGS1 PE=1 SV=1[more]
FPGS3_ARATH1.7e-11244.32Folylpolyglutamate synthase OS=Arabidopsis thaliana GN=FPGS3 PE=1 SV=1[more]
FOLC_CRIGR8.5e-6433.09Folylpolyglutamate synthase, mitochondrial OS=Cricetulus griseus GN=FPGS PE=2 SV... [more]
FOLC_HUMAN7.2e-6334.97Folylpolyglutamate synthase, mitochondrial OS=Homo sapiens GN=FPGS PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LHM9_CUCSA5.4e-25183.52Folylpolyglutamate synthase OS=Cucumis sativus GN=Csa_3G823100 PE=3 SV=1[more]
F6HLM9_VITVI8.0e-17862.20Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g05910 PE=4 SV=... [more]
B9IHV7_POPTR5.2e-17761.75Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0016s04130g PE=4 SV=2[more]
A0A061F3L1_THECC1.3e-17560.73Dhfs-fpgs c isoform 1 OS=Theobroma cacao GN=TCM_024488 PE=4 SV=1[more]
B9H9H1_POPTR2.2e-17561.45Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s04390g PE=4 SV=2[more]
Match NameE-valueIdentityDescription
AT3G10160.19.7e-16757.04 DHFS-FPGS homolog C[more]
AT5G05980.17.5e-12749.53 DHFS-FPGS homolog B[more]
AT3G55630.39.5e-11444.32 DHFS-FPGS homolog D[more]
AT5G41480.11.4e-2442.45 Folylpolyglutamate synthetase family protein[more]
Match NameE-valueIdentityDescription
gi|449437789|ref|XP_004136673.1|7.8e-25183.52PREDICTED: folylpolyglutamate synthase isoform X1 [Cucumis sativus][more]
gi|659085292|ref|XP_008443342.1|1.2e-24882.42PREDICTED: folylpolyglutamate synthase isoform X1 [Cucumis melo][more]
gi|778685402|ref|XP_011652214.1|6.8e-22383.85PREDICTED: folylpolyglutamate synthase isoform X2 [Cucumis sativus][more]
gi|659085294|ref|XP_008443343.1|1.3e-22183.02PREDICTED: folylpolyglutamate synthase isoform X2 [Cucumis melo][more]
gi|1009159846|ref|XP_015898037.1|6.3e-18462.25PREDICTED: folylpolyglutamate synthase [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016874ligase activity
GO:0005524ATP binding
GO:0004326tetrahydrofolylpolyglutamate synthase activity
Vocabulary: Biological Process
TermDefinition
GO:0009058biosynthetic process
GO:0009396folic acid-containing compound biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR018109Folylpolyglutamate_synth_CS
IPR013221Mur_ligase_cen
IPR004101Mur_ligase_C
IPR001645Folylpolyglutamate_synth
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046656 folic acid biosynthetic process
biological_process GO:0006536 glutamate metabolic process
biological_process GO:0006730 one-carbon metabolic process
biological_process GO:0046901 tetrahydrofolylpolyglutamate biosynthetic process
biological_process GO:0009058 biosynthetic process
biological_process GO:0009396 folic acid-containing compound biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004326 tetrahydrofolylpolyglutamate synthase activity
molecular_function GO:0016874 ligase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g08420.1Cp4.1LG03g08420.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001645Folylpolyglutamate synthetasePANTHERPTHR11136FOLYLPOLYGLUTAMATE SYNTHASE-RELATEDcoord: 388..531
score: 9.1E-281coord: 1..362
score: 9.1E
IPR001645Folylpolyglutamate synthetaseTIGRFAMsTIGR01499TIGR01499coord: 40..362
score: 8.1
IPR004101Mur ligase, C-terminalunknownSSF53244MurD-like peptide ligases, peptide-binding domaincoord: 325..410
score: 4.7
IPR013221Mur ligase, centralGENE3DG3DSA:3.40.1190.10coord: 9..298
score: 3.9
IPR013221Mur ligase, centralunknownSSF53623MurD-like peptide ligases, catalytic domaincoord: 31..267
score: 1.44
IPR018109Folylpolyglutamate synthetase, conserved sitePROSITEPS01012FOLYLPOLYGLU_SYNT_2coord: 160..175
scor
NoneNo IPR availablePANTHERPTHR11136:SF7FOLYLPOLYGLUTAMATE SYNTHASEcoord: 388..531
score: 9.1E-281coord: 1..362
score: 9.1E