Cp4.1LG03g05320 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG03g05320 vs. Swiss-Prot
Match: GCP4_MEDTR (Gamma-tubulin complex component 4 homolog OS=Medicago truncatula GN=85P PE=2 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 1.5e-292
Identity = 531/758 (70.05%), Postives = 605/758 (79.82%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIID R+       N+L  + PIS E TFKLAPDISF++PS+R+L
Sbjct: 1   MLHELLLALLGYTGDLIIDRRD-------NNLSANTPISDECTFKLAPDISFIDPSDREL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           I+RII  GFYYREL+RF+ KSRNL+WIRS N + L N         E PSVYRRA+ANGI
Sbjct: 61  IERIITLGFYYRELERFSAKSRNLNWIRSENANPLENK--------EKPSVYRRALANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEIL+VY S++LH+EQ LLSET+PILA VTQGL+KFF L PPL++L+L+IER DIRGG+L
Sbjct: 121 VEILAVYSSSILHIEQLLLSETMPILATVTQGLNKFFSLLPPLYELILKIERGDIRGGEL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHK+CHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGIL+D+HGEFFI RQ+GR+ 
Sbjct: 181 LNLLHKKCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILEDRHGEFFISRQEGRDV 240

Query: 241 DQGSAVQVLSEKLGRLST-DESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLR 300
           +  S+ Q +SEKL RLST D SL+DWH+GFHI LDMLP+YI M+VAESILFAGKA+RVLR
Sbjct: 241 ENSSSHQEISEKLSRLSTADASLSDWHMGFHISLDMLPEYIPMRVAESILFAGKAVRVLR 300

Query: 301 NPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIE 360
           NPS +F  Q   +  +   P+   KI G    F  Q+EP   T +  E+LL QSEADKIE
Sbjct: 301 NPSPSFLSQ---DDVYPQEPKRFPKIHGFEGRFNFQREPIINTGMRVEDLLPQSEADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
            MLLDLKESSEFHKRSFE A+DSI+AIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF
Sbjct: 361 NMLLDLKESSEFHKRSFECAVDSIQAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420

Query: 421 FQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVK- 480
           FQCFLEESR LMRLPPRQSTAEADLM+PFQLA+ KT  +ED+YFS+VSLRMPS+G+ VK 
Sbjct: 421 FQCFLEESRQLMRLPPRQSTAEADLMVPFQLASLKTIGEEDKYFSKVSLRMPSYGITVKP 480

Query: 481 SAQGDPK-EKTYTDGNPGGALSNISSDM-----------------------------YCR 540
           S    PK      DG  G ++SN SS+M                             Y +
Sbjct: 481 SLLNVPKATSAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSRYLK 540

Query: 541 VFQYLLRLKRTQMELEKSWASLMHQDHADFSKN-RNDRFDGSISPQKRQRFRQMWRVREH 600
           VFQYLLRLKRTQMELEK WAS+MHQ H+ F+KN ++D+    I+ Q+ QRFR MWRVREH
Sbjct: 541 VFQYLLRLKRTQMELEKLWASVMHQYHSIFAKNKKSDQDKSPITQQRDQRFRSMWRVREH 600

Query: 601 MAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVS 660
           MAFLIRNLQFYIQVDVIESQWNILQ  IQDSHDFTELVGFHQEYLSALISQ+FLDIGSVS
Sbjct: 601 MAFLIRNLQFYIQVDVIESQWNILQSHIQDSHDFTELVGFHQEYLSALISQTFLDIGSVS 660

Query: 661 RILDGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPF 720
           RILDGIMKLCLQFCW+IENQD  S+ SELEH+ EEFNKKSNSLYTILRSSRL GSQR PF
Sbjct: 661 RILDGIMKLCLQFCWNIENQDNFSNTSELEHIAEEFNKKSNSLYTILRSSRLAGSQRTPF 720

Query: 721 LRRFLMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 726
           LRRFL+RLN NSFFE+TA+ V+NVV PRP   P LNQ+
Sbjct: 721 LRRFLLRLNLNSFFESTAKEVMNVVRPRP-TFPGLNQR 739

BLAST of Cp4.1LG03g05320 vs. Swiss-Prot
Match: GACP4_ARATH (Gamma-tubulin complex component 4 OS=Arabidopsis thaliana GN=GCP4 PE=2 SV=2)

HSP 1 Score: 992.3 bits (2564), Expect = 2.9e-288
Identity = 527/745 (70.74%), Postives = 594/745 (79.73%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLG+TGDLI+DERE    LGL     D+P+S E TFKLAPDISF+EPSERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGLA-FNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRS-SNGSSLPNSVESSKDKTEI-PSVYRRAIAN 120
           I+R+I  GFYYRELDRFA KSRNLSWIRS ++   L  + E SK   E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGG 180
           GI EILSVYRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL++++LEIERDDIRGG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQVMYNQLA+WMVYGILQD HGEFFI+RQD  
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETDQGSAVQVLSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRV 300
           + D  S+ + +SEKL R S  E SLTDWH GFHI LDMLPDYI M++ ESILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADK 360
           LRNPS AF  Q   ++S     R   +I+G   +   + E      LTG ELL QSEADK
Sbjct: 301 LRNPSPAF--QFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGELLPQSEADK 360

Query: 361 IEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKG 420
           IEAML DLKESSEFHKRSFE  +DS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL KG
Sbjct: 361 IEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEKG 420

Query: 421 DFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKV 480
           DFFQCFLEESR LMRLPPRQST E+DLM+PFQLAATKT ++ED+YFSRVSLRMPSFGV V
Sbjct: 421 DFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVTV 480

Query: 481 KSAQGDPKEKTYTDGNPGGALSNISSD-------------------------MYCRVFQY 540
           +S+Q D      +        S+ S D                          Y +VFQY
Sbjct: 481 RSSQADMVRSKVSLTGKANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSKYLKVFQY 540

Query: 541 LLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMAFLI 600
           L+RLKRTQMELEKSWAS+MHQDH + +++R D  +GS S Q+RQ  R MWRVREHMAFLI
Sbjct: 541 LIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVREHMAFLI 600

Query: 601 RNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILDG 660
           RNLQFYIQVDVIESQW +LQ  I DS DFTELVGFHQEYLSALISQSFLDIGSVSRILD 
Sbjct: 601 RNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGSVSRILDS 660

Query: 661 IMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRFL 718
           IMKLCLQFCW+IENQ+ + + SELE++ EEFNKKSNSLYTILRSS+L GSQRAPFLRRFL
Sbjct: 661 IMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRAPFLRRFL 720

BLAST of Cp4.1LG03g05320 vs. Swiss-Prot
Match: GCP4_MOUSE (Gamma-tubulin complex component 4 OS=Mus musculus GN=Tubgcp4 PE=1 SV=2)

HSP 1 Score: 275.8 bits (704), Expect = 1.4e-72
Identity = 217/756 (28.70%), Postives = 362/756 (47.88%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL PSE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           + R+   G  Y     F  +         +      +     + +  +  +Y RA   G+
Sbjct: 61  LNRLCRLGTDYIRFTEFIEQY--------TGHVQQQDHHPPQQGQGGLHGIYLRAFCTGL 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
             +L  YR A+L +EQ+ L++    ++ V   LD+F +LFP +  +V +I+   I G Q+
Sbjct: 121 DSVLQPYRQALLDLEQEFLADPHLSISHVNYSLDQFQLLFPSVMVVVEQIKSQKIHGCQI 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           L  ++K    G+P +++ ++++L   H VMY QL++WM++G+L D+H EFFI++     T
Sbjct: 181 LETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGT 240

Query: 241 DQGSAVQVLSEKLG---------------RLSTDESLTDWHL-GFHIYLDMLPDYIHMKV 300
              + ++   E LG               RL  +E++    L  F + +++LP YI ++V
Sbjct: 241 -LSAQLEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRV 300

Query: 301 AESILFAGKAIRVLRNPSNAFWCQGA--GNQSHSHMPRLPLKIKGNTRNFPLQKEPFFAT 360
           AE ILF G+++++  N +     +G+   NQ  +    L            L+++P F+ 
Sbjct: 301 AEKILFVGESVQMFENQNVNLTRKGSILKNQEDTFAAEL----------HRLKQQPLFS- 360

Query: 361 KLTGEELLLQSEADKIEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLN 420
                              L+D           FE  +D IR+  A HLW+L+V  +DL 
Sbjct: 361 -------------------LVD-----------FEQVVDRIRSTVAEHLWKLMVEESDLL 420

Query: 421 GHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRY 480
           G LK +KD++LL +G+ FQ F++ ++H+++ PP   T E D+ + FQ +A K   D+D  
Sbjct: 421 GQLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNL 480

Query: 481 FSRVSLRMPSFGVKVKSAQGDPKEKTYTDGNP------GGALSNIS-------------- 540
              + L +   G   K+    P+E    + +P      G A   +S              
Sbjct: 481 LPLLHLTIEYHGKDHKADATQPREVPSRETSPREAPSSGWAALGLSYKVQWPLHILFTPA 540

Query: 541 -SDMYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQM 600
             + Y  VF+YLL ++R Q EL+  WA  M + H     N+ D                 
Sbjct: 541 VLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKH--LKSNQTDAV--------------K 600

Query: 601 WRVREHMAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFL 660
           WR+R HMAFL+ NLQ+Y+QVDV+ESQ++ L  +I  + DF  +   H  +LS L++QSF+
Sbjct: 601 WRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNLLAQSFI 660

Query: 661 DIGSVSRILDGIMKLCLQFCWSIENQD----KSSDPSELEHLTEEFNKKSNSLYTILRSS 714
            +  V   L+ I+ LC  FC S+ +Q+         ++L  L + F+++S+ L+ IL S 
Sbjct: 661 LLKPVFHCLNEILDLCHSFC-SLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFKILSSV 666

BLAST of Cp4.1LG03g05320 vs. Swiss-Prot
Match: GCP4_HUMAN (Gamma-tubulin complex component 4 OS=Homo sapiens GN=TUBGCP4 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 6.8e-72
Identity = 214/755 (28.34%), Postives = 359/755 (47.55%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL PSE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           + R+   G  Y     F  +         +      +   S + +  +  +Y RA   G+
Sbjct: 61  LNRLCRLGTDYIRFTEFIEQY--------TGHVQQQDHHPSQQGQGGLHGIYLRAFCTGL 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
             +L  YR A+L +EQ+ L +    ++ V   LD+F +LFP +  +V +I+   I G Q+
Sbjct: 121 DSVLQPYRQALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQI 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRR------ 240
           L  ++K    G+P +++ ++++L   H VMY QL++WM++G+L D+H EFFI++      
Sbjct: 181 LETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGN 240

Query: 241 --QDGRETDQGSAVQVLSEK-------LGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVA 300
                 E ++   +  L+ K       L  +  +  L      F + +++LP YI ++VA
Sbjct: 241 VSAQPEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRVA 300

Query: 301 ESILFAGKAIRVLRNPSNAFWCQGA--GNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATK 360
           E ILF G+++++  N +     +G+   NQ  +    L            L+++P F+  
Sbjct: 301 EKILFVGESVQMFENQNVNLTRKGSILKNQEDTFAAEL----------HRLKQQPLFS-- 360

Query: 361 LTGEELLLQSEADKIEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNG 420
                             L+D           FE  +D IR+  A HLW+L+V  +DL G
Sbjct: 361 ------------------LVD-----------FEQVVDRIRSTVAEHLWKLMVEESDLLG 420

Query: 421 HLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYF 480
            LK +KD++LL +G+ FQ F++ ++H+++ PP   T E D+ + FQ +A K   D+D   
Sbjct: 421 QLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNLL 480

Query: 481 SRVSLRMPSFGVKVKSAQGDPKEKTYTDGNP------GGALSNIS--------------- 540
             + L +   G + K+     +E    + +P      G A   +S               
Sbjct: 481 PLLHLTIEYHGKEHKADATQAREGPSRETSPREAPASGWAALGLSYKVQWPLHILFTPAV 540

Query: 541 SDMYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMW 600
            + Y  VF+YLL ++R Q EL+  WA  M + H     N+ D                 W
Sbjct: 541 LEKYNVVFKYLLSVRRVQAELQHCWALQMQRKH--LKSNQTDAI--------------KW 600

Query: 601 RVREHMAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLD 660
           R+R HMAFL+ NLQ+Y+QVDV+ESQ++ L  +I  + DF  +   H  +LS L++QSF+ 
Sbjct: 601 RLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNLLAQSFIL 660

Query: 661 IGSVSRILDGIMKLCLQFCWSIENQD----KSSDPSELEHLTEEFNKKSNSLYTILRSSR 714
           +  V   L+ I+ LC  FC S+ +Q+         ++L  L + F+++S+ L+ IL S R
Sbjct: 661 LKPVFHCLNEILDLCHSFC-SLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFKILSSVR 666

BLAST of Cp4.1LG03g05320 vs. Swiss-Prot
Match: GCP4_DROME (Gamma-tubulin complex component 4 homolog OS=Drosophila melanogaster GN=Grip75 PE=1 SV=2)

HSP 1 Score: 164.9 bits (416), Expect = 3.4e-39
Identity = 168/677 (24.82%), Postives = 302/677 (44.61%), Query Frame = 1

Query: 52  FLEPSERDLIQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSV 111
           F+ P ER++   II     Y+E+++F   S      +S     LP+S+            
Sbjct: 31  FIHPCEREIFMDIIKIIKVYQEVEQFTHSSGR----KSDTHGELPDSLHG---------Y 90

Query: 112 YRRAIANGIVEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIE 171
           Y   +A GI   L  Y + +  +E+  L      L+ V   L   F L   +  L+ EI 
Sbjct: 91  YLLNLAKGIEMALEEYYAEIGRLEKYCLGNERNSLSYVYNALYAKFPLLVFMRNLITEIH 150

Query: 172 RDDIRGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFF 231
             ++RG  LL+ LH++C  G  +L+  I+ ++       ++ LA W+++G++ D H EFF
Sbjct: 151 VLNLRGCVLLHNLHQQCEHGDIQLEKAIKIIMKPVKNAFFSSLAHWLLFGVIDDVHSEFF 210

Query: 232 IRRQDGRETDQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFA 291
           I+       D  S  +  S     LS +++  D+   + + +  LP +  + +AE +LF 
Sbjct: 211 IKFTPTDAVDGSSFSK--SATCSLLSAEKNPEDYIWQYEVNMSQLPGFFSIVLAEKVLFV 270

Query: 292 GKAIRVLRNPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEEL-- 351
           G+ + V +   N                   +K+K  T   PL  +     +L  +++  
Sbjct: 271 GQTVLVFKMGRN-------------------VKVKNKTD--PLAAK---LAELDSDDIYQ 330

Query: 352 LLQSEADKIEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALK 411
           L      +   M++DL      +    E  I  I+   ++ L ++ V   DL   +  +K
Sbjct: 331 LWSGRESEFFKMVVDLSNEDTINVFRLEKVIIDIKNYVSARLSEIAVNEVDLERQMGLIK 390

Query: 412 DYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKT--TSDEDRY--FSR 471
           D+FLL +G+F+  F   S+ +  +   +     ++   F+LAAT T  T D D++    +
Sbjct: 391 DFFLLGRGEFYLEFC--SQMVGTMETYREERFKNVTRSFELAATVTGITDDLDKFSLICQ 450

Query: 472 VSLRMPSFGVKVKSAQGDPKEKTYTDGNPGGALSNISSDMYCRVFQYLLRLKRTQMELEK 531
            S   P         QG   +  Y +       S  + + Y  +F++LL ++  Q E+++
Sbjct: 451 RSTSEPDDTSDFNFLQGLSLKYEY-EWPLNLLFSPTTIERYNNIFRFLLIIRTYQYEIQR 510

Query: 532 SWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMAFLIRNLQFYIQVDVIE 591
            WA    +     +K+  D     + P  +     +  +R ++ F + N+Q+YIQVDV+E
Sbjct: 511 VWAKQTWR-----AKSAKD-----VPPNNK-----IITLRNYLMFFLNNMQYYIQVDVLE 570

Query: 592 SQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFL---------DIGSVSR--ILDGIM 651
           SQ+ IL + I+   DF  +   H  +L+ ++S  FL           GS +R  I   ++
Sbjct: 571 SQFGILMNVIKSRSDFEVIQRAHTVFLANVLSHCFLLNESETQLNVTGSQNRNPIYGTLL 630

Query: 652 KL---CLQFCWSIENQDKSSD-PSELEHLTEEFNKKSNSLYTIL---RSSRLVGSQRAPF 705
           KL   C +F    + +D S D   E++ L E F  +  SL  +L   +S+  +G      
Sbjct: 631 KLFGICEKFAHMTQTKDPSDDLEDEVDQLNESFGVQIASLIQLLVDVKSASCLGP----- 645

BLAST of Cp4.1LG03g05320 vs. TrEMBL
Match: M5VMI9_PRUPE (Gamma-tubulin complex component OS=Prunus persica GN=PRUPE_ppa001912mg PE=3 SV=1)

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 578/756 (76.46%), Postives = 637/756 (84.26%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREH   LG+     DAPIS EPTFKLAPDISF++PSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHSKSLGV-----DAPISDEPTFKLAPDISFIQPSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           I++I   GFYYRELDRF+ +SRNLSWIRS+N SSL + +  SK K E PSVYRRAIANGI
Sbjct: 61  IEKITTLGFYYRELDRFSVRSRNLSWIRSANASSLASDL--SKPKAEKPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVLH+EQKLLSETVPILA VTQGL+KFFVL PPL++LVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHIEQKLLSETVPILATVTQGLNKFFVLLPPLYELVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHKRCHCGVPELQTCIQRLLWH HQV+YNQLA+WMVYGILQD+HGEFFIRRQ+ ++ 
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHAHQVLYNQLAAWMVYGILQDQHGEFFIRRQEDKDV 240

Query: 241 DQGSAVQVLSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLR 300
           + GS+ + +SEKL R+STD+ SLTDWHLGFHIYLDMLP+YIHM+VAESILF+GKAIRVLR
Sbjct: 241 EHGSSHRDISEKLARMSTDDTSLTDWHLGFHIYLDMLPEYIHMRVAESILFSGKAIRVLR 300

Query: 301 NPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIE 360
           NPS+AF  Q      H  +PR   K++G +  FP  K+PF   +L GEELL QSEADKIE
Sbjct: 301 NPSHAFRFQDI--VYHQQVPRGSHKVQGFSGRFPFLKDPFADKELIGEELLPQSEADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
            MLL+LKESSEFHKRSFE AIDSIRA AASHLWQLVVVRADLNGHLKALKDYFLLAKGDF
Sbjct: 361 TMLLELKESSEFHKRSFECAIDSIRATAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420

Query: 421 FQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKS 480
           FQCFLEESR LMRLPPRQSTAEADLM+PFQLAA KT S+ED+YFSRVSLRMPSFG+ VKS
Sbjct: 421 FQCFLEESRQLMRLPPRQSTAEADLMVPFQLAAIKTISEEDKYFSRVSLRMPSFGMTVKS 480

Query: 481 AQGD-PKEKTYTDGNPGGALSNISSDM-----------------------------YCRV 540
           +Q D PK    TDGN G ALS+ SS++                             YCRV
Sbjct: 481 SQVDLPK----TDGNAGTALSSASSEISLDGWDGIALEYSVDWPLQLFFTPDVLSKYCRV 540

Query: 541 FQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMA 600
           FQYLLRLKRTQMELEKSWAS+MHQDH DF+K+RND   GS+S Q RQR R MWR+REHMA
Sbjct: 541 FQYLLRLKRTQMELEKSWASVMHQDHTDFAKHRNDHVKGSVSQQGRQRSRPMWRIREHMA 600

Query: 601 FLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660
           FLIRNLQFYIQVDVIESQWN+LQ  IQDS+DFT LVG HQEYLSALISQSFLDIGS+SRI
Sbjct: 601 FLIRNLQFYIQVDVIESQWNVLQAHIQDSNDFTGLVGVHQEYLSALISQSFLDIGSLSRI 660

Query: 661 LDGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLR 720
           LD IMKLCLQFCW+IENQ+ S++ SELEH+ EEFNKKSNSLYTILRSSRLVGSQRAPFLR
Sbjct: 661 LDSIMKLCLQFCWNIENQESSANTSELEHIIEEFNKKSNSLYTILRSSRLVGSQRAPFLR 720

Query: 721 RFLMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 726
           RFLMRLNFNSFFEATA+GVLNVV PRP  L VLNQQ
Sbjct: 721 RFLMRLNFNSFFEATAKGVLNVVRPRP-TLSVLNQQ 742

BLAST of Cp4.1LG03g05320 vs. TrEMBL
Match: A0A061DQM0_THECC (Gamma-tubulin complex component OS=Theobroma cacao GN=TCM_004669 PE=3 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 569/756 (75.26%), Postives = 633/756 (83.73%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLAL+GYTGDLIIDEREH+  LGL HL PDAPIS + +FKLA DISF++ SERDL
Sbjct: 1   MLHELLLALVGYTGDLIIDEREHHKSLGL-HLSPDAPISDQRSFKLASDISFIDSSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           I+R+I  GFYYRELDRFATKSRNLSWIRS++ S L  +   SK KTE PSVYRRAIANG+
Sbjct: 61  IERLITLGFYYRELDRFATKSRNLSWIRSADVSPLERAAGLSKPKTEKPSVYRRAIANGL 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVL +EQKLLSET+PILA VTQGL+KFFV+ PPL++L+LEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLQIEQKLLSETMPILATVTQGLNKFFVILPPLYELILEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHKRCHCGVPELQ CIQRLLWHGHQV+YNQLASWMVYGILQD+HGEFFIRRQ+ R+ 
Sbjct: 181 LNLLHKRCHCGVPELQACIQRLLWHGHQVLYNQLASWMVYGILQDQHGEFFIRRQEDRDV 240

Query: 241 DQGSAVQVLSEKLGRLSTDES-LTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLR 300
           D GS++  +SEKL RLSTD+S LTDWHLGFHI+LDMLP+YI M VAESILFAGKA+RVLR
Sbjct: 241 DYGSSISDMSEKLARLSTDDSSLTDWHLGFHIFLDMLPEYIPMHVAESILFAGKAVRVLR 300

Query: 301 NPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIE 360
           NPS AF  Q A    +  + +   K++G++   P  KEPF   K+ GEELL QSEADKIE
Sbjct: 301 NPSPAFQFQDA--LRNQQIKKGSQKVQGSSGRVPFHKEPFLDIKMIGEELLPQSEADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
            ML DLKESSEFHKRSFE ++DSIRA+AASHLWQLVVVRADLNGHLKALKDYFLLAKGDF
Sbjct: 361 TMLQDLKESSEFHKRSFECSVDSIRAVAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420

Query: 421 FQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKS 480
           FQCFLEESRHLMRLPPRQSTAEADLM+PFQLAA KT S+ED+YFS VSLRMPSFG+ VKS
Sbjct: 421 FQCFLEESRHLMRLPPRQSTAEADLMVPFQLAAIKTISEEDKYFSLVSLRMPSFGITVKS 480

Query: 481 AQGD-PKEKTYTDGNPGGALSNIS-----------------------------SDMYCRV 540
           +Q D P  KTYTDG+ G  LS+ S                                Y R+
Sbjct: 481 SQVDLPNTKTYTDGSSGAVLSSTSSETSLDGWDGIALEYSVDWPLQLFFTQEVLSKYRRI 540

Query: 541 FQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMA 600
           FQYLLRLKRTQMELEKSWAS+MHQDH DF+K+RNDR + SIS  +RQR R MW VREHMA
Sbjct: 541 FQYLLRLKRTQMELEKSWASVMHQDHTDFAKHRNDRMNCSISQPRRQRCRPMWHVREHMA 600

Query: 601 FLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660
           FLIRNLQFYIQVDVIESQWN+LQ  IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI
Sbjct: 601 FLIRNLQFYIQVDVIESQWNVLQSHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660

Query: 661 LDGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLR 720
           LD IM +CLQFCW+IENQ+ S + SELE +TEEFNKKSNSLYTILRSSRL GSQRAPFLR
Sbjct: 661 LDSIMTVCLQFCWNIENQESSQNTSELERITEEFNKKSNSLYTILRSSRLAGSQRAPFLR 720

Query: 721 RFLMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 726
           RFL+R+NFNSFFEATARGVLNVV PRP +LPVLNQ+
Sbjct: 721 RFLLRMNFNSFFEATARGVLNVVRPRP-SLPVLNQR 752

BLAST of Cp4.1LG03g05320 vs. TrEMBL
Match: A0A067JWA3_JATCU (Gamma-tubulin complex component OS=Jatropha curcas GN=JCGZ_13943 PE=3 SV=1)

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 578/752 (76.86%), Postives = 624/752 (82.98%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREH   +G+  L PDA IS E  FKLAPDISF+EPS+RDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHQKSIGVR-LSPDASISDERCFKLAPDISFIEPSDRDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRII  GFYYRELDRFATKSRNLSWIRSSN S L  + E S + TE PSVYRRAIANG+
Sbjct: 61  IQRIISLGFYYRELDRFATKSRNLSWIRSSNVSPLARATELSHNTTEKPSVYRRAIANGV 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVLH+EQKLLSETVPILA +TQGL+KFFVL PPL++LVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHIEQKLLSETVPILATITQGLNKFFVLLPPLYELVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWM+YGILQD+HGEFFIRRQ+ R+ 
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMIYGILQDQHGEFFIRRQEDRDV 240

Query: 241 DQGSAVQVLSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLR 300
              S+   +SEKL RLSTDE  LTDWHLGFHIYLDMLP+YIHM+VAESILFAGKAIRVL+
Sbjct: 241 KHNSSPD-MSEKLARLSTDEMPLTDWHLGFHIYLDMLPEYIHMRVAESILFAGKAIRVLQ 300

Query: 301 NPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIE 360
           NPS AF  Q      +  MPR   KI G +  FP QKEPF  T L GEELL QSEADKIE
Sbjct: 301 NPSPAF--QFKDPSHNQQMPRGAQKIHGLSGRFPFQKEPFVDTNLIGEELLPQSEADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
           AML  LKESSEFHKRSFE AIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF
Sbjct: 361 AMLQGLKESSEFHKRSFECAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420

Query: 421 FQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKS 480
           FQCF+EESR LMRLPPRQSTAEADLM+PFQLAA KT S+ED+YF RVSLRMPSFG+ VKS
Sbjct: 421 FQCFIEESRQLMRLPPRQSTAEADLMVPFQLAALKTISEEDKYFYRVSLRMPSFGITVKS 480

Query: 481 AQGD-PKEKTYTDGNPGGALSNISSDM-----------------------------YCRV 540
           +  D PK K Y+D   G ALSN SS+M                             Y +V
Sbjct: 481 SPADQPKSKVYSD---GAALSNASSEMSLDGWDGIALEYAVDWPLQLFFTQEVLSKYLKV 540

Query: 541 FQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMA 600
           FQYLLRLKRTQMELEKSWASLMHQDH DF+K R DR   S   ++RQRFR MW +REHMA
Sbjct: 541 FQYLLRLKRTQMELEKSWASLMHQDHTDFAKRRKDR-KNSTPHEQRQRFRPMWHIREHMA 600

Query: 601 FLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660
           FLIRNLQFYIQVDVIESQWN+LQ  IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI
Sbjct: 601 FLIRNLQFYIQVDVIESQWNVLQAHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660

Query: 661 LDGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLR 720
           LD IMKLCLQ+CWS+ENQ+ + + SEL+H+TEEFNKKSNSLYTILRSSRL GSQRAPFLR
Sbjct: 661 LDSIMKLCLQYCWSMENQENNPNTSELDHITEEFNKKSNSLYTILRSSRLAGSQRAPFLR 720

Query: 721 RFLMRLNFNSFFEATARGVLNVVGPRPAALPV 722
           RFL+RLNFNSFFEATARGVLNVV P P  LPV
Sbjct: 721 RFLLRLNFNSFFEATARGVLNVVRPSP-TLPV 743

BLAST of Cp4.1LG03g05320 vs. TrEMBL
Match: B9SAS5_RICCO (Gamma-tubulin complex component OS=Ricinus communis GN=RCOM_1178380 PE=3 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 575/754 (76.26%), Postives = 633/754 (83.95%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDER H   +G+ HL PDA IS E +FKLAPDISF++PS+RDL
Sbjct: 1   MLHELLLALLGYTGDLIIDERGHQKSIGV-HLSPDASISDERSFKLAPDISFIDPSDRDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVE-SSKDKTEIPSVYRRAIANG 120
           I+RII  GFYYRELDRFATKSRNLSWIRS+N S L  + E SS + T+  SVYRRAIANG
Sbjct: 61  IERIITLGFYYRELDRFATKSRNLSWIRSTNVSPLSRANELSSNNNTQKQSVYRRAIANG 120

Query: 121 IVEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQ 180
           IVEILSVYRSAVLH+EQKLLSETVPILA VTQGL+KFFVL PPL++LVLEIERDDIRGGQ
Sbjct: 121 IVEILSVYRSAVLHIEQKLLSETVPILATVTQGLNKFFVLLPPLYELVLEIERDDIRGGQ 180

Query: 181 LLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRE 240
           LLNLLHKR HCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQD+HGEFFI RQ+ R+
Sbjct: 181 LLNLLHKRSHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDQHGEFFITRQEDRD 240

Query: 241 TDQGSAVQVLSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVL 300
               SA   +SEKL RLSTD+ SLTDWHLGFHI+LDMLP+YIHM VAES+LFAGKAIRVL
Sbjct: 241 LVNSSAQPDMSEKLARLSTDDISLTDWHLGFHIFLDMLPEYIHMCVAESVLFAGKAIRVL 300

Query: 301 RNPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKI 360
           RNPS AF C+ + +  +  +P+    I+G    FP+QKEPF  + L GEELL QSEADKI
Sbjct: 301 RNPSPAFQCKDSLH--NQQVPKGGQNIQGFVGRFPVQKEPFVDSNLIGEELLPQSEADKI 360

Query: 361 EAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGD 420
           EA+L  LKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGD
Sbjct: 361 EALLQGLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGD 420

Query: 421 FFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVK 480
           FFQCFLEESR LMRLPPRQSTAEADLM+PFQLAA KT  +ED+YFSRVSLRMPSFG+ VK
Sbjct: 421 FFQCFLEESRQLMRLPPRQSTAEADLMVPFQLAAIKTIGEEDKYFSRVSLRMPSFGITVK 480

Query: 481 SAQGD-PKEKTYTDGNPGGALSNISSDM-----------------------------YCR 540
           S+Q D PK K ++D N G ALSN +S+M                             Y R
Sbjct: 481 SSQVDLPKSKAHSDSNSGAALSNAASEMFIDGWDGIALEYAVDWPLQLFFTQEVLSKYLR 540

Query: 541 VFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHM 600
           VFQYLLRLKRTQMELEKSWAS+MHQDH DF+K  NDR + SIS Q+RQRFR MWRVREHM
Sbjct: 541 VFQYLLRLKRTQMELEKSWASVMHQDHTDFAKRHNDR-NCSISQQRRQRFRPMWRVREHM 600

Query: 601 AFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSR 660
           AFLIRNLQFYIQVDVIESQWN+LQ  IQDSHDFTELVGFHQEYLSAL+SQSFLDIGSVSR
Sbjct: 601 AFLIRNLQFYIQVDVIESQWNVLQAHIQDSHDFTELVGFHQEYLSALVSQSFLDIGSVSR 660

Query: 661 ILDGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFL 720
           ILD IM+LCLQFCWSIENQ+ + + SEL+H+TEEFNKKSNSLYTILRSSRL GSQRAPFL
Sbjct: 661 ILDSIMRLCLQFCWSIENQESNPNTSELDHITEEFNKKSNSLYTILRSSRLAGSQRAPFL 720

Query: 721 RRFLMRLNFNSFFEATARGVLNVVGPRPAALPVL 723
           RRFL+RLN+N+FFEATARGVLNVV P P  LP L
Sbjct: 721 RRFLLRLNYNAFFEATARGVLNVVRPSP-TLPSL 749

BLAST of Cp4.1LG03g05320 vs. TrEMBL
Match: V4VW99_9ROSI (Gamma-tubulin complex component OS=Citrus clementina GN=CICLE_v10018978mg PE=3 SV=1)

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 568/756 (75.13%), Postives = 627/756 (82.94%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREH N      L  DAPIS E +FKLAPDISF+EPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHQNSAA-TPLSGDAPISEECSFKLAPDISFIEPSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           +++II  GFYYRE+DRFA+K+RNLSWIRS+N S L  + E SK KTE PSVYRRAIANGI
Sbjct: 61  LEKIITLGFYYREIDRFASKARNLSWIRSANASPLERAAELSKSKTERPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVL +EQKLLSET+PILA VTQGL+KFFVLFPPL++LVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLLIEQKLLSETMPILATVTQGLNKFFVLFPPLYELVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHKRCHCG+PELQ CIQRLLWHGHQVMYNQLASWMVYGILQD+HGEFFIRRQ+ R+T
Sbjct: 181 LNLLHKRCHCGMPELQACIQRLLWHGHQVMYNQLASWMVYGILQDQHGEFFIRRQEDRDT 240

Query: 241 DQGSAVQVLSEKLGRLSTD-ESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLR 300
           +Q S+   +SEKL RLSTD  S+TDWHLGFHI+LDMLP+YIHM++ ESILFAGKA+RVLR
Sbjct: 241 EQCSSHSDVSEKLARLSTDYTSVTDWHLGFHIFLDMLPEYIHMRIGESILFAGKAVRVLR 300

Query: 301 NPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIE 360
           NPS+AF  Q         + R   KI+G T  F +QKEP    KL GEELL QS+ADKIE
Sbjct: 301 NPSSAFRFQ------DQLITRSSQKIQGVTGRFSIQKEPLLDMKLIGEELLPQSDADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
           AML  LKE SEFHKRSFE A+DSIRAIAASHLWQLVVVRA+LNGHLKALKDYFLL KGDF
Sbjct: 361 AMLQGLKEPSEFHKRSFECAVDSIRAIAASHLWQLVVVRANLNGHLKALKDYFLLEKGDF 420

Query: 421 FQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKS 480
           FQCFLEESR LMRLPPRQSTAEADLM+PFQLAA KT S+ED+YFSRVSLRMPSFG+ VKS
Sbjct: 421 FQCFLEESRQLMRLPPRQSTAEADLMVPFQLAAIKTISEEDKYFSRVSLRMPSFGITVKS 480

Query: 481 AQGD-PKEKTYTDGNPGGALSNISSDM-----------------------------YCRV 540
              D  K K Y DG  G ALS+ +SD+                             YC+V
Sbjct: 481 PHVDLLKAKAYADGTSGAALSSGNSDISLDGWDGIALEYSVDWPLQLFFTQEVLSKYCKV 540

Query: 541 FQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMA 600
           FQYLLRLKRTQMELEKSWAS+MHQDH  F+K+RNDR + S S Q+RQ  R MWRVREHMA
Sbjct: 541 FQYLLRLKRTQMELEKSWASVMHQDHTYFAKHRNDRINYSKSQQRRQTCRPMWRVREHMA 600

Query: 601 FLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660
           FLIRNLQFYIQVDVIE+QWNILQ  IQ+SHDFTELVGFHQEYLSALISQSFLD+GSVSRI
Sbjct: 601 FLIRNLQFYIQVDVIETQWNILQAHIQESHDFTELVGFHQEYLSALISQSFLDLGSVSRI 660

Query: 661 LDGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLR 720
           LDGIMKLC+QFCW+IENQ+ S +  ELEH+TEEFNKKSNSLYTILRS+RL GSQRAPFLR
Sbjct: 661 LDGIMKLCIQFCWAIENQESSENTPELEHITEEFNKKSNSLYTILRSTRLAGSQRAPFLR 720

Query: 721 RFLMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 726
           RFL+RLNFNSFFE TARGVLNVV  RP A+ VLNQQ
Sbjct: 721 RFLLRLNFNSFFEMTARGVLNVVRQRP-AITVLNQQ 748

BLAST of Cp4.1LG03g05320 vs. TAIR10
Match: AT3G53760.1 (AT3G53760.1 GAMMA-TUBULIN COMPLEX PROTEIN 4)

HSP 1 Score: 992.3 bits (2564), Expect = 1.6e-289
Identity = 527/745 (70.74%), Postives = 594/745 (79.73%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLG+TGDLI+DERE    LGL     D+P+S E TFKLAPDISF+EPSERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGLA-FNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRS-SNGSSLPNSVESSKDKTEI-PSVYRRAIAN 120
           I+R+I  GFYYRELDRFA KSRNLSWIRS ++   L  + E SK   E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGG 180
           GI EILSVYRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL++++LEIERDDIRGG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQVMYNQLA+WMVYGILQD HGEFFI+RQD  
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETDQGSAVQVLSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRV 300
           + D  S+ + +SEKL R S  E SLTDWH GFHI LDMLPDYI M++ ESILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADK 360
           LRNPS AF  Q   ++S     R   +I+G   +   + E      LTG ELL QSEADK
Sbjct: 301 LRNPSPAF--QFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGELLPQSEADK 360

Query: 361 IEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKG 420
           IEAML DLKESSEFHKRSFE  +DS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL KG
Sbjct: 361 IEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEKG 420

Query: 421 DFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKV 480
           DFFQCFLEESR LMRLPPRQST E+DLM+PFQLAATKT ++ED+YFSRVSLRMPSFGV V
Sbjct: 421 DFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVTV 480

Query: 481 KSAQGDPKEKTYTDGNPGGALSNISSD-------------------------MYCRVFQY 540
           +S+Q D      +        S+ S D                          Y +VFQY
Sbjct: 481 RSSQADMVRSKVSLTGKANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSKYLKVFQY 540

Query: 541 LLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMAFLI 600
           L+RLKRTQMELEKSWAS+MHQDH + +++R D  +GS S Q+RQ  R MWRVREHMAFLI
Sbjct: 541 LIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVREHMAFLI 600

Query: 601 RNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILDG 660
           RNLQFYIQVDVIESQW +LQ  I DS DFTELVGFHQEYLSALISQSFLDIGSVSRILD 
Sbjct: 601 RNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGSVSRILDS 660

Query: 661 IMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRFL 718
           IMKLCLQFCW+IENQ+ + + SELE++ EEFNKKSNSLYTILRSS+L GSQRAPFLRRFL
Sbjct: 661 IMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRAPFLRRFL 720

BLAST of Cp4.1LG03g05320 vs. TAIR10
Match: AT5G06680.1 (AT5G06680.1 spindle pole body component 98)

HSP 1 Score: 87.8 bits (216), Expect = 3.0e-17
Identity = 104/457 (22.76%), Postives = 178/457 (38.95%), Query Frame = 1

Query: 175 IRGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRR 234
           +RGG +   +H     G P +   +  LL      ++  + SW++ G L+D  GEFF+  
Sbjct: 334 LRGGAMAGAIHLHAQHGDPLVHDFMMSLLRCVCSPLFEMVRSWVLEGELEDTFGEFFV-- 393

Query: 235 QDGRETDQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKA 294
                  Q   V +L               W  G+ ++  MLP +I   +A+ IL  GK+
Sbjct: 394 -----VGQPVKVDLL---------------WREGYKLHPAMLPSFISPSLAQRILRTGKS 453

Query: 295 IRVLRNPSNAF-WCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQS 354
           I  LR   +   W   A   + +          G T            T+  G   L   
Sbjct: 454 INFLRVCCDDHGWADAASEAAAA---------SGTT------------TRRGG---LGYG 513

Query: 355 EADKIEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFL 414
           E D +E ++      +E  KR     ID        HL  ++  R     H  A+K Y L
Sbjct: 514 ETDALEHLV------TEAAKR-----ID-------KHLLDVLYKRYKFKEHCLAIKRYLL 573

Query: 415 LAKGDFFQCFLEESRHLMRLPPRQ-STAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPS 474
           L +GDF Q  ++     +  P    S+ E    +   + A+    D+     R+ ++M  
Sbjct: 574 LGQGDFVQYLMDIVGPKLSEPANNISSFELAGFLEAAIRASNAQYDDRDMLDRLRVKMMP 633

Query: 475 FG--------VKVKSAQGDPKEKTYTDGNPGGALSNISSDMYCRVFQYLLRLKRTQMELE 534
            G          ++     P +  +T+      LS      Y RVF +L +LKR +  L 
Sbjct: 634 HGSGDRGWDVFSLEYEARVPLDTVFTE----SVLSK-----YLRVFNFLWKLKRVEHALI 693

Query: 535 KSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMAFLIRNLQFYIQVDVI 594
             W ++        + N   +   S+  Q     R+   +   M   + N Q+YI  +V+
Sbjct: 694 GIWKTMKPN---CITSNSFVKLQSSVKLQLLSALRRCQVLWNEMNHFVTNFQYYIMFEVL 714

Query: 595 ESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFL 622
           E  W+     ++ + D  +L+  H++YL+A++ +S L
Sbjct: 754 EVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLL 714

BLAST of Cp4.1LG03g05320 vs. TAIR10
Match: AT5G17410.2 (AT5G17410.2 Spc97 / Spc98 family of spindle pole body (SBP) component)

HSP 1 Score: 54.3 bits (129), Expect = 3.6e-07
Identity = 72/341 (21.11%), Postives = 135/341 (39.59%), Query Frame = 1

Query: 380 IDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQ-S 439
           I +    A+  L  L+  + DL G L+++K Y LL +GDF   F++ +R  +     + S
Sbjct: 342 IKAAHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEIS 401

Query: 440 TAEADLMIPFQLAATKTTSD---EDR--YFSRVSLRMPSFGVKVKSAQGDPKEKTYTDGN 499
             +   ++   L  T   +D   ED      R SL + + G+   +     ++     G 
Sbjct: 402 VEKLQSLLDLALRTTAAAADPRHEDLTCCVDRASL-LTTLGMHKDTDSNSIEDPMSITGL 461

Query: 500 PGGAL------------SNISSDMYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFSKN 559
              +L            S  +   Y  +F++L   K  + +L  +W   +HQ     +  
Sbjct: 462 ETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAWQ--IHQGIRSMNSK 521

Query: 560 RNDRFDGSISPQKRQRFRQMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDF 619
                  S+  +   +F             I +L  Y+  +V+E  W+++ DR+Q +   
Sbjct: 522 GTAILRSSLLCRSMLKF-------------ISSLLHYLTFEVLEPNWHVMHDRLQSTRSV 581

Query: 620 TELVGFHQEYLSALISQSFLDIGSVSRILDGIMKLCLQFC----WSIENQ---DKSSDPS 679
            E++  H  +L   +    L +  V + ++ +  +CLQ+     W I +    +  S P 
Sbjct: 582 DEVIQHHDFFLDKCLRGCLLLLPDVLKKMEKLKSVCLQYAAATQWLISSSIDINSQSHPQ 641

Query: 680 EL----EHLTE---EFNKKSNSLYTILRSSRLVGSQRAPFL 689
           +       +TE    F ++ NS    L      GSQ  P+L
Sbjct: 642 KTMIRDTTVTESIFNFEREFNSELQSLGPVLSKGSQAEPYL 666

BLAST of Cp4.1LG03g05320 vs. NCBI nr
Match: gi|778663742|ref|XP_011660150.1| (PREDICTED: gamma-tubulin complex component 4 homolog [Cucumis sativus])

HSP 1 Score: 1329.7 bits (3440), Expect = 0.0e+00
Identity = 677/754 (89.79%), Postives = 695/754 (92.18%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGL++L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRIIV GFYYRELDRFATKSRNLSWIRS N SSL NS ESSKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSE VPILAIVTQGLDKFFVLFPPLHQL+ EIERDDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSEMVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHS+MPRLPL +KGNTRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYF RVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFCRVSLRMPSFGVKVKSA 480

Query: 481 QGDPKEKTYTDGNPGGALSNISSDM-----------------------------YCRVFQ 540
           QGD KEK   DGNPGGALSN+S DM                             YCRVFQ
Sbjct: 481 QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF+ NR  +FDGSIS Q+RQRFR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQD SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 726
           LMRLNFNSFFEATARGVLNVV PRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754

BLAST of Cp4.1LG03g05320 vs. NCBI nr
Match: gi|659130829|ref|XP_008465374.1| (PREDICTED: gamma-tubulin complex component 4 homolog [Cucumis melo])

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 616/691 (89.15%), Postives = 634/691 (91.75%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGLN+L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRIIV GFYYRELDRFATKSRNLSWIRS N SSL NS ESSKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIERDDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHS+MPRLPL +KGNTRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQSAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDPKEKTYTDGNPGGAL-SNISSDM-----------------------------YCRVF 540
           QGD KEK   DGNPGGAL SN+S DM                             YCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADF+ NRN +FDGSIS Q+RQRFR+MWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDKSSDPSELEHLTE 662
           DGIMKLCLQFCWSIENQD SSDPSELEHLTE
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTE 691

BLAST of Cp4.1LG03g05320 vs. NCBI nr
Match: gi|659099008|ref|XP_008450387.1| (PREDICTED: gamma-tubulin complex component 4 homolog isoform X1 [Cucumis melo])

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 615/691 (89.00%), Postives = 632/691 (91.46%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGLN+L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRIIV GFYYRELDRFATKSRNLSWIRS N SSL  S ESSKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLAISTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIERDDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLICEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHS+MPRLPL +KGN RNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNARNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDPKEKTYTDGNPGGAL-SNISSDM-----------------------------YCRVF 540
           QGD KEK   DGNPGGAL SN+S DM                             YCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSPDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADF+  RN +FDGSIS Q+RQRFR+MWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANIRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDKSSDPSELEHLTE 662
           DGIMKLCLQFCWSIENQD SSDPSELEHLTE
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTE 691

BLAST of Cp4.1LG03g05320 vs. NCBI nr
Match: gi|595796847|ref|XP_007201205.1| (hypothetical protein PRUPE_ppa001912mg [Prunus persica])

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 578/756 (76.46%), Postives = 637/756 (84.26%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREH   LG+     DAPIS EPTFKLAPDISF++PSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHSKSLGV-----DAPISDEPTFKLAPDISFIQPSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           I++I   GFYYRELDRF+ +SRNLSWIRS+N SSL + +  SK K E PSVYRRAIANGI
Sbjct: 61  IEKITTLGFYYRELDRFSVRSRNLSWIRSANASSLASDL--SKPKAEKPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVLH+EQKLLSETVPILA VTQGL+KFFVL PPL++LVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHIEQKLLSETVPILATVTQGLNKFFVLLPPLYELVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHKRCHCGVPELQTCIQRLLWH HQV+YNQLA+WMVYGILQD+HGEFFIRRQ+ ++ 
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHAHQVLYNQLAAWMVYGILQDQHGEFFIRRQEDKDV 240

Query: 241 DQGSAVQVLSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLR 300
           + GS+ + +SEKL R+STD+ SLTDWHLGFHIYLDMLP+YIHM+VAESILF+GKAIRVLR
Sbjct: 241 EHGSSHRDISEKLARMSTDDTSLTDWHLGFHIYLDMLPEYIHMRVAESILFSGKAIRVLR 300

Query: 301 NPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIE 360
           NPS+AF  Q      H  +PR   K++G +  FP  K+PF   +L GEELL QSEADKIE
Sbjct: 301 NPSHAFRFQDI--VYHQQVPRGSHKVQGFSGRFPFLKDPFADKELIGEELLPQSEADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
            MLL+LKESSEFHKRSFE AIDSIRA AASHLWQLVVVRADLNGHLKALKDYFLLAKGDF
Sbjct: 361 TMLLELKESSEFHKRSFECAIDSIRATAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420

Query: 421 FQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKS 480
           FQCFLEESR LMRLPPRQSTAEADLM+PFQLAA KT S+ED+YFSRVSLRMPSFG+ VKS
Sbjct: 421 FQCFLEESRQLMRLPPRQSTAEADLMVPFQLAAIKTISEEDKYFSRVSLRMPSFGMTVKS 480

Query: 481 AQGD-PKEKTYTDGNPGGALSNISSDM-----------------------------YCRV 540
           +Q D PK    TDGN G ALS+ SS++                             YCRV
Sbjct: 481 SQVDLPK----TDGNAGTALSSASSEISLDGWDGIALEYSVDWPLQLFFTPDVLSKYCRV 540

Query: 541 FQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMA 600
           FQYLLRLKRTQMELEKSWAS+MHQDH DF+K+RND   GS+S Q RQR R MWR+REHMA
Sbjct: 541 FQYLLRLKRTQMELEKSWASVMHQDHTDFAKHRNDHVKGSVSQQGRQRSRPMWRIREHMA 600

Query: 601 FLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660
           FLIRNLQFYIQVDVIESQWN+LQ  IQDS+DFT LVG HQEYLSALISQSFLDIGS+SRI
Sbjct: 601 FLIRNLQFYIQVDVIESQWNVLQAHIQDSNDFTGLVGVHQEYLSALISQSFLDIGSLSRI 660

Query: 661 LDGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLR 720
           LD IMKLCLQFCW+IENQ+ S++ SELEH+ EEFNKKSNSLYTILRSSRLVGSQRAPFLR
Sbjct: 661 LDSIMKLCLQFCWNIENQESSANTSELEHIIEEFNKKSNSLYTILRSSRLVGSQRAPFLR 720

Query: 721 RFLMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 726
           RFLMRLNFNSFFEATA+GVLNVV PRP  L VLNQQ
Sbjct: 721 RFLMRLNFNSFFEATAKGVLNVVRPRP-TLSVLNQQ 742

BLAST of Cp4.1LG03g05320 vs. NCBI nr
Match: gi|590719039|ref|XP_007050947.1| (Tubulin gamma complex-associated protein isoform 1 [Theobroma cacao])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 569/756 (75.26%), Postives = 633/756 (83.73%), Query Frame = 1

Query: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLAL+GYTGDLIIDEREH+  LGL HL PDAPIS + +FKLA DISF++ SERDL
Sbjct: 1   MLHELLLALVGYTGDLIIDEREHHKSLGL-HLSPDAPISDQRSFKLASDISFIDSSERDL 60

Query: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           I+R+I  GFYYRELDRFATKSRNLSWIRS++ S L  +   SK KTE PSVYRRAIANG+
Sbjct: 61  IERLITLGFYYRELDRFATKSRNLSWIRSADVSPLERAAGLSKPKTEKPSVYRRAIANGL 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVL +EQKLLSET+PILA VTQGL+KFFV+ PPL++L+LEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLQIEQKLLSETMPILATVTQGLNKFFVILPPLYELILEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240
           LNLLHKRCHCGVPELQ CIQRLLWHGHQV+YNQLASWMVYGILQD+HGEFFIRRQ+ R+ 
Sbjct: 181 LNLLHKRCHCGVPELQACIQRLLWHGHQVLYNQLASWMVYGILQDQHGEFFIRRQEDRDV 240

Query: 241 DQGSAVQVLSEKLGRLSTDES-LTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLR 300
           D GS++  +SEKL RLSTD+S LTDWHLGFHI+LDMLP+YI M VAESILFAGKA+RVLR
Sbjct: 241 DYGSSISDMSEKLARLSTDDSSLTDWHLGFHIFLDMLPEYIPMHVAESILFAGKAVRVLR 300

Query: 301 NPSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIE 360
           NPS AF  Q A    +  + +   K++G++   P  KEPF   K+ GEELL QSEADKIE
Sbjct: 301 NPSPAFQFQDA--LRNQQIKKGSQKVQGSSGRVPFHKEPFLDIKMIGEELLPQSEADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
            ML DLKESSEFHKRSFE ++DSIRA+AASHLWQLVVVRADLNGHLKALKDYFLLAKGDF
Sbjct: 361 TMLQDLKESSEFHKRSFECSVDSIRAVAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420

Query: 421 FQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKS 480
           FQCFLEESRHLMRLPPRQSTAEADLM+PFQLAA KT S+ED+YFS VSLRMPSFG+ VKS
Sbjct: 421 FQCFLEESRHLMRLPPRQSTAEADLMVPFQLAAIKTISEEDKYFSLVSLRMPSFGITVKS 480

Query: 481 AQGD-PKEKTYTDGNPGGALSNIS-----------------------------SDMYCRV 540
           +Q D P  KTYTDG+ G  LS+ S                                Y R+
Sbjct: 481 SQVDLPNTKTYTDGSSGAVLSSTSSETSLDGWDGIALEYSVDWPLQLFFTQEVLSKYRRI 540

Query: 541 FQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMA 600
           FQYLLRLKRTQMELEKSWAS+MHQDH DF+K+RNDR + SIS  +RQR R MW VREHMA
Sbjct: 541 FQYLLRLKRTQMELEKSWASVMHQDHTDFAKHRNDRMNCSISQPRRQRCRPMWHVREHMA 600

Query: 601 FLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660
           FLIRNLQFYIQVDVIESQWN+LQ  IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI
Sbjct: 601 FLIRNLQFYIQVDVIESQWNVLQSHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRI 660

Query: 661 LDGIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLR 720
           LD IM +CLQFCW+IENQ+ S + SELE +TEEFNKKSNSLYTILRSSRL GSQRAPFLR
Sbjct: 661 LDSIMTVCLQFCWNIENQESSQNTSELERITEEFNKKSNSLYTILRSSRLAGSQRAPFLR 720

Query: 721 RFLMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 726
           RFL+R+NFNSFFEATARGVLNVV PRP +LPVLNQ+
Sbjct: 721 RFLLRMNFNSFFEATARGVLNVVRPRP-SLPVLNQR 752