Cp4.1LG03g05240 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG03g05240
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Invertase/pectin methylesterase inhibitor family protein
Location	Cp4.1LG03 : 4896027 .. 4896575 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTTATCTCAGCCATTGTGGTTGTTCTCTGCCTGTGCATGGCGGCAAAGCGCCACGACGCCACCCGCCCCGTGGAGGAAGGGATGGCCCTAATTGAGGAAATGTGTAGCCAAACCAAACACAAGGAGCTGTGCGTGGACTCCCTGACCTCGGACCCCAACAGCTACCCGGCGGACAAGACGGGGCTAGCCCTGGTGGCCCTCCGCTTCGCCTCCTCCAACGCCTCGGACATCTCGGAGAGCATCAAGGTGATGTTCAATGACTCGACCCTGGCCAAGCCGATGGTGCAGGAGGCTCTCCTGGACTGCCTCGATGAGTACCTAGACGCGTCCCAACAGCTGGACGACTCCATTGCGGCGCTGACGGTGAAGGCGTATGGGGACCTGGAGAAATGGGTGAATATAGCCATGACGGATGTCCGCACGTGTGGGACGGAGTTGGGCAGCGAGCGCCCACCGCTGCTGACGGAGAGGAATGACGTGTTTATTAAGCTCTGTGAAATTGCCCTGACCATTTCCAAGATTGCGCAGGGTGAAGGGAACTGA

mRNA sequence

ATGAAATTTATCTCAGCCATTGTGGTTGTTCTCTGCCTGTGCATGGCGGCAAAGCGCCACGACGCCACCCGCCCCGTGGAGGAAGGGATGGCCCTAATTGAGGAAATGTGTAGCCAAACCAAACACAAGGAGCTGTGCGTGGACTCCCTGACCTCGGACCCCAACAGCTACCCGGCGGACAAGACGGGGCTAGCCCTGGTGGCCCTCCGCTTCGCCTCCTCCAACGCCTCGGACATCTCGGAGAGCATCAAGGTGATGTTCAATGACTCGACCCTGGCCAAGCCGATGGTGCAGGAGGCTCTCCTGGACTGCCTCGATGAGTACCTAGACGCGTCCCAACAGCTGGACGACTCCATTGCGGCGCTGACGGTGAAGGCGTATGGGGACCTGGAGAAATGGGTGAATATAGCCATGACGGATGTCCGCACGTGTGGGACGGAGTTGGGCAGCGAGCGCCCACCGCTGCTGACGGAGAGGAATGACGTGTTTATTAAGCTCTGTGAAATTGCCCTGACCATTTCCAAGATTGCGCAGGGTGAAGGGAACTGA

Coding sequence (CDS)

ATGAAATTTATCTCAGCCATTGTGGTTGTTCTCTGCCTGTGCATGGCGGCAAAGCGCCACGACGCCACCCGCCCCGTGGAGGAAGGGATGGCCCTAATTGAGGAAATGTGTAGCCAAACCAAACACAAGGAGCTGTGCGTGGACTCCCTGACCTCGGACCCCAACAGCTACCCGGCGGACAAGACGGGGCTAGCCCTGGTGGCCCTCCGCTTCGCCTCCTCCAACGCCTCGGACATCTCGGAGAGCATCAAGGTGATGTTCAATGACTCGACCCTGGCCAAGCCGATGGTGCAGGAGGCTCTCCTGGACTGCCTCGATGAGTACCTAGACGCGTCCCAACAGCTGGACGACTCCATTGCGGCGCTGACGGTGAAGGCGTATGGGGACCTGGAGAAATGGGTGAATATAGCCATGACGGATGTCCGCACGTGTGGGACGGAGTTGGGCAGCGAGCGCCCACCGCTGCTGACGGAGAGGAATGACGTGTTTATTAAGCTCTGTGAAATTGCCCTGACCATTTCCAAGATTGCGCAGGGTGAAGGGAACTGA

Protein sequence

MKFISAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTLAKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQGEGN

Homology

[Close]

BLAST of Cp4.1LG03g05240 vs. Swiss-Prot
Match: PMEI_ACTDE (Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-10
Identity = 53/181 (29.28%), Postives = 86/181 (47.51%), Query Frame = 1


Query: 3   FISAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPADKT 62
           F+S ++V+L +   ++R      V+    LI E+C +T++  LC+ +L SDP S   D  
Sbjct: 11  FVSLLLVILFISPLSQRPS----VKAENHLISEICPKTRNPSLCLQALESDPRSASKDLK 70

Query: 63  GLALVALRFASSNASDISESIKVMFNDSTLAKPMVQEALLDCLDEYLDASQQLDDSIAAL 122
           GL   ++  A ++A   S+ I  + N +T   P ++     C + Y DA   L  +   L
Sbjct: 71  GLGQFSIDIAQASAKQTSKIIASLTNQAT--DPKLKGRYETCSENYADAIDSLGQAKQFL 130

Query: 123 TVKAYGDLEKWVNIAMTDVRTCGTELGSERPP-LLTERNDVFIK---LCEIALTISKIAQ 180
           T   Y  L  + + A     TC  E   E PP + T+ +   +K   LC+I L IS +  
Sbjct: 131 TSGDYNSLNIYASAAFDGAGTC--EDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLP 183

[Close]

BLAST of Cp4.1LG03g05240 vs. Swiss-Prot
Match: PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.1e-07
Identity = 37/135 (27.41%), Postives = 66/135 (48.89%), Query Frame = 1


Query: 44  ELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTLAKPMVQEALLD 103
           + CV SL +DP S+ AD  GL +++   A  + S I   I  +        P +++ L D
Sbjct: 44  DFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTFIGRILKSK--VDPALKKYLND 103

Query: 104 CLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPL--LTERND 163
           C+  Y DA   + ++IA    K Y      ++ A+ D  TC      ++  +  +T+ N 
Sbjct: 104 CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENK 163

Query: 164 VFIKLCEIALTISKI 177
            +++L  I+L I+K+
Sbjct: 164 DYVQLTAISLAITKL 176

[Close]

BLAST of Cp4.1LG03g05240 vs. TrEMBL
Match: A0A0A0KY96_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001930 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-56
Identity = 117/180 (65.00%), Postives = 149/180 (82.78%), Query Frame = 1


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPV-EEGMALIEEMCSQTKHKELCVDSLTSDPNSYPA 60
           MKF SAI++VLCLCMA +R DA     EEG+ +I++MC+QT +K+LC+ SLTSDPNS+PA
Sbjct: 1   MKFFSAILLVLCLCMAPQRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60

Query: 61  DKTGLALVALRFASSNASDISESIKVMFND-STLAKPMVQEALLDCLDEYLDASQQLDDS 120
           DK GLALVALR ASSNASDISESIKVM N+ S   +P VQ+AL DCLDEYL+ASQQLDDS
Sbjct: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120

Query: 121 IAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           IAA+  KAYGD+++WV +A+T+VRTC +   + +P +LT RN+ FIKLC+IAL+I+KIA+
Sbjct: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPT-KPSVLTPRNEEFIKLCDIALSITKIAE 179

[Close]

BLAST of Cp4.1LG03g05240 vs. TrEMBL
Match: W9QQ78_9ROSA (Putative invertase inhibitor OS=Morus notabilis GN=L484_011114 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-30
Identity = 73/144 (50.69%), Postives = 97/144 (67.36%), Query Frame = 1


Query: 33  IEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTL 92
           +E+ C    HK+ C+  L SDPNS+ +D  GL L+ALR ASSNASDISE IKV+ NDS+L
Sbjct: 35  LEKACQHAGHKDSCISMLQSDPNSHSSDVKGLTLIALRLASSNASDISEHIKVLLNDSSL 94

Query: 93  AKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSER 152
             P VQ+ L +CL+ YLDA++QLDDS+AAL   AY D+E WVN+A+ D  +C + L    
Sbjct: 95  -DPAVQDGLSECLEYYLDAAEQLDDSVAALLANAYKDVEAWVNVAIIDAASCDSALDGHE 154

Query: 153 PPLLTERNDVFIKLCEIALTISKI 177
             +L  + D F  LC+ AL I K+
Sbjct: 155 -FVLGGKTDAFRGLCDNALAIIKV 176

[Close]

BLAST of Cp4.1LG03g05240 vs. TrEMBL
Match: M5VKT0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011794mg PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-26
Identity = 65/184 (35.33%), Postives = 108/184 (58.70%), Query Frame = 1


Query: 3   FISAIVVVLCLCMAAKRHDATRPVEEG----MALIEEMCSQTKHKELCVDSLTSDPNSYP 62
           F+S + V+  + +A      T    +     M LI++ C    HK+LC+D L +DPNS  
Sbjct: 11  FLSTLSVLQFVLVAGHESQGTNGGSKAGVVNMNLIQQACQHAPHKDLCIDILKNDPNSKG 70

Query: 63  ADKTGLALVALRFASSNASDISESIK-VMFNDSTLAKPMVQEALLDCLDEYLDASQQLDD 122
           AD TGLA +A+R A + AS++   ++ ++ N++T   P+VQ+ + DC++ Y DA++QLDD
Sbjct: 71  ADLTGLAYIAIRLAGAYASEVDAHLRSLLINNATTLSPVVQQGVADCIEHYSDANEQLDD 130

Query: 123 SIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIA 182
            IAA++ K + D+E WVN+A++D   C      E+  +L ++N+ F +LC   L + K  
Sbjct: 131 CIAAMSTKNFKDVEVWVNVAISDADYCDDSFSGEKSVVL-QKNEAFRQLCNNVLAVVKAL 190

[Close]

BLAST of Cp4.1LG03g05240 vs. TrEMBL
Match: A0A061DYQ4_THECC (Plant invertase/pectin methylesterase inhibitor superfamily protein, putative OS=Theobroma cacao GN=TCM_004694 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.9e-23
Identity = 66/146 (45.21%), Postives = 92/146 (63.01%), Query Frame = 1


Query: 32  LIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDST 91
           LI E C + ++K+LC+ SL ++  S  AD   LAL+AL+ AS+N SD S  IK + + S 
Sbjct: 32  LIAEACQRARYKDLCISSLEAEHASQDADLAALALIALKVASNNGSDTSVYIKKILDGSN 91

Query: 92  LAKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSE 151
           L +P V++   DC + YL A+QQLDDS+AAL  KAY D + W+  A+TD  TC  E G +
Sbjct: 92  L-EPTVEQNFQDCSENYLSATQQLDDSLAALVSKAYKDAKVWLEAAITDAITC--ESGLK 151

Query: 152 RPP----LLTERNDVFIKLCEIALTI 174
           + P     L  RN++F+KLC  AL I
Sbjct: 152 QSPGNELELFSRNNIFLKLCSNALDI 174

[Close]

BLAST of Cp4.1LG03g05240 vs. TrEMBL
Match: B9H1C2_POPTR (Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa GN=POPTR_0004s01630g PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.9e-22
Identity = 63/148 (42.57%), Postives = 90/148 (60.81%), Query Frame = 1


Query: 32  LIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDST 91
           LI+E+C++T +K  CV SL S+P+S  A+   L ++AL  AS+NA++ S  IK     + 
Sbjct: 31  LIQEVCTKTHNKVNCVASLESNPDSKQANLQQLGIIALNLASTNATNTSSYIKTTLLSNK 90

Query: 92  LAKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSE 151
              P+ ++AL DC D+YLDA QQLDDS+AAL   A  D+  WV  A+ DV +C      +
Sbjct: 91  TLGPVNEQALEDCSDQYLDAIQQLDDSLAALLANATNDVRAWVRAAVADVESCENGFKKQ 150

Query: 152 RP---PLLTERNDVFIKLCEIALTISKI 177
            P    LL+ RN VF +LC   L I+K+
Sbjct: 151 VPGQQMLLSSRNAVFRQLCNNVLVINKL 178

[Close]

BLAST of Cp4.1LG03g05240 vs. TAIR10
Match: AT1G55770.1 (AT1G55770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 103.2 bits (256), Expect = 1.7e-22
Identity = 62/176 (35.23%), Postives = 104/176 (59.09%), Query Frame = 1


Query: 5   SAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNS--YPADKT 64
           SA V+ LC+ + +     T     GM  I ++C  +    LC+ S+TS P S  +     
Sbjct: 4   SAGVLFLCIVLISF---VTGNANSGM--ISDLCKHSDDPNLCLSSITSRPESGEFAGTSN 63

Query: 65  GLALVALRFASSNASDISESIKVMFNDSTLAKPMVQEALLDCLDEYLDASQQLDDSIAAL 124
            + ++A+  AS+NAS  S  IK   ++  L +P +++ L DC  +Y DA +QLDDSI+A+
Sbjct: 64  QIEIIAISAASANASATSSYIKQKLSNEDL-EPAIEDTLEDCQKDYQDAVEQLDDSISAM 123

Query: 125 TVKAYGDLEKWVNIAMTDVRTCGTELGSE--RPPLLTERNDVFIKLCEIALTISKI 177
              A+ D++ W++ A++ + +CG+ LGS       L++RN+VF+KLC+ AL I+K+
Sbjct: 124 LADAHTDVDVWLSAAISAIESCGSALGSRAGNDAELSQRNEVFLKLCKNALMINKM 173

[Close]

BLAST of Cp4.1LG03g05240 vs. TAIR10
Match: AT4G02250.1 (AT4G02250.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 91.3 bits (225), Expect = 6.7e-19
Identity = 56/136 (41.18%), Postives = 74/136 (54.41%), Query Frame = 1


Query: 43  KELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTLAKPMVQEALL 102
           K  CV SLTS P +  A    L ++AL  ASSNASD S  IK       L +P +++ L 
Sbjct: 23  KAFCVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNL-EPALEDTLD 82

Query: 103 DCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSE--RPPLLTERN 162
           DC   YLDA  QLDDS+AAL   ++ D++ W+N A++D   C   L         L  RN
Sbjct: 83  DCSKNYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRN 142

Query: 163 DVFIKLCEIALTISKI 177
              +KLC+ AL I+ I
Sbjct: 143 TNLLKLCKDALLINTI 157

[Close]

BLAST of Cp4.1LG03g05240 vs. TAIR10
Match: AT3G17220.1 (AT3G17220.1 pectin methylesterase inhibitor 2)

HSP 1 Score: 51.2 bits (121), Expect = 7.7e-07
Identity = 37/148 (25.00%), Postives = 68/148 (45.95%), Query Frame = 1


Query: 30  MALIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFND 89
           +A I+ +C + K++  C   + S+P +  AD   LA +    A ++AS+    I+ +   
Sbjct: 27  VADIKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKT 86

Query: 90  STLAKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELG 149
           +T   P +++A   C+  Y  A   L+D+  +L       L   V+ AM    TC  ++ 
Sbjct: 87  AT--NPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMA 146

Query: 150 SER-PPLLTERNDVFIKLCEIALTISKI 177
             +  P   + +  F  +C I L IS +
Sbjct: 147 DFKVDPSAVKNSGDFQNICGIVLVISNM 172

[Close]

BLAST of Cp4.1LG03g05240 vs. TAIR10
Match: AT5G46940.1 (AT5G46940.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 49.3 bits (116), Expect = 2.9e-06
Identity = 46/184 (25.00%), Postives = 81/184 (44.02%), Query Frame = 1


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQT-----KHK-ELCVDSLTSDP 60
           MKF+  +V    L         +  +  G  LI   C +      K K  LCV SL ++P
Sbjct: 1   MKFLLYLVTFFVL---------SNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNP 60

Query: 61  NSYPA-DKTGLALVALRFASSNASDISESIKVMFNDSTLAKPMVQEALLDCLDEYLDASQ 120
            +  A D  GL + + + A + A+ +  ++  +     + K M    L DCL  Y DA  
Sbjct: 61  QAKTAKDLAGLVMASTKNAVTKATTLKGTVDKIIKGKKVNK-MTAMPLRDCLQLYTDAIG 120

Query: 121 QLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPL-LTERNDVFIKLCEIALT 177
            L++++A +  + Y  ++  ++ AM    TC T     + P  +T+ ND   ++  I L 
Sbjct: 121 SLNEALAGVKSRNYPTVKTVLSAAMDTPSTCETGFKERKAPSPVTKENDNLYQMILIPLA 174

[Close]

BLAST of Cp4.1LG03g05240 vs. NCBI nr
Match: gi|778689341|ref|XP_004152360.2| (PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus])

HSP 1 Score: 227.6 bits (579), Expect = 1.7e-56
Identity = 117/180 (65.00%), Postives = 149/180 (82.78%), Query Frame = 1


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPV-EEGMALIEEMCSQTKHKELCVDSLTSDPNSYPA 60
           MKF SAI++VLCLCMA +R DA     EEG+ +I++MC+QT +K+LC+ SLTSDPNS+PA
Sbjct: 1   MKFFSAILLVLCLCMAPQRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60

Query: 61  DKTGLALVALRFASSNASDISESIKVMFND-STLAKPMVQEALLDCLDEYLDASQQLDDS 120
           DK GLALVALR ASSNASDISESIKVM N+ S   +P VQ+AL DCLDEYL+ASQQLDDS
Sbjct: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120

Query: 121 IAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           IAA+  KAYGD+++WV +A+T+VRTC +   + +P +LT RN+ FIKLC+IAL+I+KIA+
Sbjct: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPT-KPSVLTPRNEEFIKLCDIALSITKIAE 179

[Close]

BLAST of Cp4.1LG03g05240 vs. NCBI nr
Match: gi|659109003|ref|XP_008454497.1| (PREDICTED: uncharacterized protein LOC103494896 [Cucumis melo])

HSP 1 Score: 156.0 bits (393), Expect = 6.3e-35
Identity = 79/116 (68.10%), Postives = 96/116 (82.76%), Query Frame = 1


Query: 63  GLALVALRFASSNASDISESIKVMFNDSTLAKPMVQEALLDCLDEYLDASQQLDDSIAAL 122
           GLALVALR ASSNASDISESIKVM N+++  +P VQ+ L DCLDEYLDASQQLDDSIAA+
Sbjct: 2   GLALVALRLASSNASDISESIKVMLNETSQNEPAVQQGLFDCLDEYLDASQQLDDSIAAI 61

Query: 123 TVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
             KAYGD+EKWV+ A+ DVRTC     + +P +LT RN+ FIKLC+IAL+IS+IA+
Sbjct: 62  IAKAYGDVEKWVHAAVADVRTCENSFPT-KPSVLTPRNEEFIKLCDIALSISRIAE 116

[Close]

BLAST of Cp4.1LG03g05240 vs. NCBI nr
Match: gi|1009148279|ref|XP_015891852.1| (PREDICTED: putative invertase inhibitor [Ziziphus jujuba])

HSP 1 Score: 146.4 bits (368), Expect = 5.0e-32
Identity = 77/174 (44.25%), Postives = 113/174 (64.94%), Query Frame = 1


Query: 3   FISAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPADKT 62
           F+S++  + C   +   +++      G  LIE  C     ++LCV +L +DPN++ AD  
Sbjct: 9   FLSSLCFLSCSSQSVFANESNAT---GTNLIETACQHAGQRDLCVTTLEADPNTHDADML 68

Query: 63  GLALVALRFASSNASDISESIKVMFNDSTLAKPMVQEALLDCLDEYLDASQQLDDSIAAL 122
           GLAL+ALR ASSNASDISE  K++F D+TL +P +Q+   +CLD YLDA++QLDDSIAAL
Sbjct: 69  GLALIALRLASSNASDISEHAKLLFKDTTL-EPTIQDGYGECLDHYLDAAEQLDDSIAAL 128

Query: 123 TVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKI 177
             KAY D+E WV +A++D   C + L  + P ++ E+N +F +LC   L I K+
Sbjct: 129 LTKAYKDVEVWVKVAISDADQCESALKGKEPAMV-EKNQIFRQLCNNVLAIVKV 177

[Close]

BLAST of Cp4.1LG03g05240 vs. NCBI nr
Match: gi|703068674|ref|XP_010088298.1| (Putative invertase inhibitor [Morus notabilis])

HSP 1 Score: 141.4 bits (355), Expect = 1.6e-30
Identity = 73/144 (50.69%), Postives = 97/144 (67.36%), Query Frame = 1


Query: 33  IEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTL 92
           +E+ C    HK+ C+  L SDPNS+ +D  GL L+ALR ASSNASDISE IKV+ NDS+L
Sbjct: 35  LEKACQHAGHKDSCISMLQSDPNSHSSDVKGLTLIALRLASSNASDISEHIKVLLNDSSL 94

Query: 93  AKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSER 152
             P VQ+ L +CL+ YLDA++QLDDS+AAL   AY D+E WVN+A+ D  +C + L    
Sbjct: 95  -DPAVQDGLSECLEYYLDAAEQLDDSVAALLANAYKDVEAWVNVAIIDAASCDSALDGHE 154

Query: 153 PPLLTERNDVFIKLCEIALTISKI 177
             +L  + D F  LC+ AL I K+
Sbjct: 155 -FVLGGKTDAFRGLCDNALAIIKV 176

[Close]

BLAST of Cp4.1LG03g05240 vs. NCBI nr
Match: gi|470112897|ref|XP_004292666.1| (PREDICTED: putative invertase inhibitor [Fragaria vesca subsp. vesca])

HSP 1 Score: 133.3 bits (334), Expect = 4.4e-28
Identity = 66/151 (43.71%), Postives = 100/151 (66.23%), Query Frame = 1


Query: 32  LIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIK-VMFNDS 91
           L+EE C Q  +K+LC++SL  DPNS  AD TGLA +ALR A++NA+D+   ++ +M  ++
Sbjct: 48  LLEEACKQATNKDLCINSLKKDPNSQGADLTGLAFIALRLAAANAADVESHLRTLMLKNA 107

Query: 92  TLAKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGS 151
           T   P VQ+ + DC+D Y DA++QLDDS+AAL+ K   D+  WV +A+ D   C + L  
Sbjct: 108 TGLDPDVQQGVQDCIDSYTDANEQLDDSVAALSDKKCKDVVVWVKVAIADSNYCESSLDG 167

Query: 152 ERPPLLTERNDVFIKLCEIALTISKIAQGEG 182
           E P +L ++N VFI++CE  L++ K    +G
Sbjct: 168 E-PTVLADKNKVFIQMCENVLSVIKALAPKG 197

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g05240 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PMEI_ACTDE	1.4e-10	29.28	Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2	[more]
PLA1_PLAAC	1.1e-07	27.41	Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1	[more]

BLAST of Cp4.1LG03g05240 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KY96_CUCSA	1.2e-56	65.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001930 PE=4 SV=1	[more]
W9QQ78_9ROSA	1.1e-30	50.69	Putative invertase inhibitor OS=Morus notabilis GN=L484_011114 PE=4 SV=1	[more]
M5VKT0_PRUPE	2.2e-26	35.33	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011794mg PE=4 SV=1	[more]
A0A061DYQ4_THECC	3.9e-23	45.21	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative OS...	[more]
B9H1C2_POPTR	1.9e-22	42.57	Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa ...	[more]

BLAST of Cp4.1LG03g05240 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G55770.1	1.7e-22	35.23	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G02250.1	6.7e-19	41.18	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G17220.1	7.7e-07	25.00	pectin methylesterase inhibitor 2	[more]
AT5G46940.1	2.9e-06	25.00	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Cp4.1LG03g05240 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778689341\|ref\|XP_004152360.2\|	1.7e-56	65.00	PREDICTED: cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus]	[more]
gi\|659109003\|ref\|XP_008454497.1\|	6.3e-35	68.10	PREDICTED: uncharacterized protein LOC103494896 [Cucumis melo]	[more]
gi\|1009148279\|ref\|XP_015891852.1\|	5.0e-32	44.25	PREDICTED: putative invertase inhibitor [Ziziphus jujuba]	[more]
gi\|703068674\|ref\|XP_010088298.1\|	1.6e-30	50.69	Putative invertase inhibitor [Morus notabilis]	[more]
gi\|470112897\|ref\|XP_004292666.1\|	4.4e-28	43.71	PREDICTED: putative invertase inhibitor [Fragaria vesca subsp. vesca]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0030245	cellulose catabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0008810	cellulase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g05240.1	Cp4.1LG03g05240.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 31..175 score: 5.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..173 score: 6.0
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..173 score: 1.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 5..176 score: 7.5
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 31..175 score: 9.94
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 3..176 score: 4.9
None	No IPR available	PANTHER	PTHR31707:SF63	INVERTASE-RELATED	coord: 3..176 score: 4.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG03g05240	Cucsa.096970	Cucumber (Gy14) v1	cgycpeB0198
Cp4.1LG03g05240	CmaCh14G009140	Cucurbita maxima (Rimu)	cmacpeB283
Cp4.1LG03g05240	CmoCh14G009410	Cucurbita moschata (Rifu)	cmocpeB245
Cp4.1LG03g05240	Cla017394	Watermelon (97103) v1	cpewmB595
Cp4.1LG03g05240	Csa4G001930	Cucumber (Chinese Long) v2	cpecuB616
Cp4.1LG03g05240	MELO3C017944	Melon (DHL92) v3.5.1	cpemeB589
Cp4.1LG03g05240	ClCG10G011070	Watermelon (Charleston Gray)	cpewcgB544
Cp4.1LG03g05240	CSPI04G00970	Wild cucumber (PI 183967)	cpecpiB618
Cp4.1LG03g05240	Lsi03G010880	Bottle gourd (USVL1VR-Ls)	cpelsiB500
Cp4.1LG03g05240	MELO3C017944.2	Melon (DHL92) v3.6.1	cpemedB699
Cp4.1LG03g05240	CsaV3_4G000950	Cucumber (Chinese Long) v3	cpecucB0768
Cp4.1LG03g05240	Bhi11G001029	Wax gourd	cpewgoB0742
Cp4.1LG03g05240	CsGy4G000850	Cucumber (Gy14) v2	cgybcpeB553
Cp4.1LG03g05240	Carg11141	Silver-seed gourd	carcpeB1147

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG03g05240	Cucurbita pepo (Zucchini)	cpecpeB336
Cp4.1LG03g05240	Cucurbita pepo (Zucchini)	cpecpeB449
Cp4.1LG03g05240	Cucurbita maxima (Rimu)	cmacpeB494
Cp4.1LG03g05240	Cucurbita moschata (Rifu)	cmocpeB453
Cp4.1LG03g05240	Silver-seed gourd	carcpeB1073
Cp4.1LG03g05240	Wax gourd	cpewgoB0819