Cp4.1LG03g04020 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG03g04020
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionE3 ubiquitin-protein ligase RNF5
LocationCp4.1LG03 : 1952877 .. 1954157 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGATGAAATCTTTGATGCAATGAACCTTGACTTGAATCTGGGTCCTATTCCAGAGCCGCTTTCGAACTCGGTTCTTCAAATTGAAGATGTGAATTTAGCTGATTTGACTGAGGATCCTGTTCATAGATTTGGGGAAGCTATCAGGTTTAGGACGAGACAGCGGTGGCGAAGGCGTCAAGTCCAAATTCCTTCTGAGACTCAGAATATTACAATGGAATTGAGTGAAATCATGGTCAATTCAGGCAATGGGAATACATTGCAGCCATTACAGGCTGGTGAGGGCAGTATCACTGCTGAGGAAAGGAAGAACGAAGCGGGGAAAACATGTGAAATCAACAATGAAGCTCTGGAAGATGAAAAGACAGAGAATAAGAGTGATGTTGAAAAGGGCAGCGATTCTGATGGGAGTTTCTTTGATTGTAATATATGTCTGGATTTGGCTAGGGACCCCGTTGTAACTTGTTGCGGTCATCTATACTGTTGGCCGTGCCTTTATCGATGGTTACATCTACATTCAGATGCCAAGGAATGCCCTGTATGTAAAGGAGAGGTCACTACAAAAAACGTAACTCCAATCTATGGGCGTGGTGGTAATACCCCAGTGACAGAGGAGGATGCTACACTTAAGATTCCTCTAAGGCCCCACGCTCGGAAAGTTGAAAGTTTGAGGCAAACCATCCAGAGGACTGCATTGAGTTTCCCAGTGGAGGATATGATTCGTCGATTGGGAAACAGATTTGATTTTAATCGTGACCTTGACCAACCGCTCGAACCAGACAGCTCACACGAAACCTTTGGTAGATCTCCAACCTTGTTGAATAGGCTTTTAACATCCAGGGGAATTCGTAGAGAACAAATGTCACTGCCCCCCCATGATGAAGTAGGAGATTTAGCACACGCCGATGTGTCTGGCATTGAGACTGGGGAAACCCGTCTTCAGTCTCTCCCCGTGCTTCGATCATTACTACACCGGACAAGAGTTTCTTCTCTTACTTCTGCATTTAACTCCGCTGATAGGCTTCCAGATGGTCTTCTATATACCGATCCGCTCATTCCTAGAATTCAAGAGCAGCCTCCTCCACCTGTTGAGGATAGAGATTCATTCTCTAGCATTGCTGCTGTTATCAATTCAGAGAGTCAAATGGACACTGCTGTAGAAATTGATTCCATGGTCTCCATTTCAACTTCATCCTCCAGAAGGAGAACTGATGCCTTACAGGTTTCTGATGTCGACAGTGGAAACTCTCGGGCACCCAGGCGGAGAAGATTGGCTTGA

mRNA sequence

ATGGGTGATGAAATCTTTGATGCAATGAACCTTGACTTGAATCTGGGTCCTATTCCAGAGCCGCTTTCGAACTCGGTTCTTCAAATTGAAGATGTGAATTTAGCTGATTTGACTGAGGATCCTGTTCATAGATTTGGGGAAGCTATCAGGTTTAGGACGAGACAGCGGTGGCGAAGGCGTCAAGTCCAAATTCCTTCTGAGACTCAGAATATTACAATGGAATTGAGTGAAATCATGGTCAATTCAGGCAATGGGAATACATTGCAGCCATTACAGGCTGGTGAGGGCAGTATCACTGCTGAGGAAAGGAAGAACGAAGCGGGGAAAACATGTGAAATCAACAATGAAGCTCTGGAAGATGAAAAGACAGAGAATAAGAGTGATGTTGAAAAGGGCAGCGATTCTGATGGGAGTTTCTTTGATTGTAATATATGTCTGGATTTGGCTAGGGACCCCGTTGTAACTTGTTGCGGTCATCTATACTGTTGGCCGTGCCTTTATCGATGGTTACATCTACATTCAGATGCCAAGGAATGCCCTGTATGTAAAGGAGAGGTCACTACAAAAAACGTAACTCCAATCTATGGGCGTGGTGGTAATACCCCAGTGACAGAGGAGGATGCTACACTTAAGATTCCTCTAAGGCCCCACGCTCGGAAAGTTGAAAGTTTGAGGCAAACCATCCAGAGGACTGCATTGAGTTTCCCAGTGGAGGATATGATTCGTCGATTGGGAAACAGATTTGATTTTAATCGTGACCTTGACCAACCGCTCGAACCAGACAGCTCACACGAAACCTTTGGTAGATCTCCAACCTTGTTGAATAGGCTTTTAACATCCAGGGGAATTCGTAGAGAACAAATGTCACTGCCCCCCCATGATGAAGTAGGAGATTTAGCACACGCCGATGTGTCTGGCATTGAGACTGGGGAAACCCGTCTTCAGTCTCTCCCCGTGCTTCGATCATTACTACACCGGACAAGAGTTTCTTCTCTTACTTCTGCATTTAACTCCGCTGATAGGCTTCCAGATGGTCTTCTATATACCGATCCGCTCATTCCTAGAATTCAAGAGCAGCCTCCTCCACCTGTTGAGGATAGAGATTCATTCTCTAGCATTGCTGCTGTTATCAATTCAGAGAGTCAAATGGACACTGCTGTAGAAATTGATTCCATGGTCTCCATTTCAACTTCATCCTCCAGAAGGAGAACTGATGCCTTACAGGTTTCTGATGTCGACAGTGGAAACTCTCGGGCACCCAGGCGGAGAAGATTGGCTTGA

Coding sequence (CDS)

ATGGGTGATGAAATCTTTGATGCAATGAACCTTGACTTGAATCTGGGTCCTATTCCAGAGCCGCTTTCGAACTCGGTTCTTCAAATTGAAGATGTGAATTTAGCTGATTTGACTGAGGATCCTGTTCATAGATTTGGGGAAGCTATCAGGTTTAGGACGAGACAGCGGTGGCGAAGGCGTCAAGTCCAAATTCCTTCTGAGACTCAGAATATTACAATGGAATTGAGTGAAATCATGGTCAATTCAGGCAATGGGAATACATTGCAGCCATTACAGGCTGGTGAGGGCAGTATCACTGCTGAGGAAAGGAAGAACGAAGCGGGGAAAACATGTGAAATCAACAATGAAGCTCTGGAAGATGAAAAGACAGAGAATAAGAGTGATGTTGAAAAGGGCAGCGATTCTGATGGGAGTTTCTTTGATTGTAATATATGTCTGGATTTGGCTAGGGACCCCGTTGTAACTTGTTGCGGTCATCTATACTGTTGGCCGTGCCTTTATCGATGGTTACATCTACATTCAGATGCCAAGGAATGCCCTGTATGTAAAGGAGAGGTCACTACAAAAAACGTAACTCCAATCTATGGGCGTGGTGGTAATACCCCAGTGACAGAGGAGGATGCTACACTTAAGATTCCTCTAAGGCCCCACGCTCGGAAAGTTGAAAGTTTGAGGCAAACCATCCAGAGGACTGCATTGAGTTTCCCAGTGGAGGATATGATTCGTCGATTGGGAAACAGATTTGATTTTAATCGTGACCTTGACCAACCGCTCGAACCAGACAGCTCACACGAAACCTTTGGTAGATCTCCAACCTTGTTGAATAGGCTTTTAACATCCAGGGGAATTCGTAGAGAACAAATGTCACTGCCCCCCCATGATGAAGTAGGAGATTTAGCACACGCCGATGTGTCTGGCATTGAGACTGGGGAAACCCGTCTTCAGTCTCTCCCCGTGCTTCGATCATTACTACACCGGACAAGAGTTTCTTCTCTTACTTCTGCATTTAACTCCGCTGATAGGCTTCCAGATGGTCTTCTATATACCGATCCGCTCATTCCTAGAATTCAAGAGCAGCCTCCTCCACCTGTTGAGGATAGAGATTCATTCTCTAGCATTGCTGCTGTTATCAATTCAGAGAGTCAAATGGACACTGCTGTAGAAATTGATTCCATGGTCTCCATTTCAACTTCATCCTCCAGAAGGAGAACTGATGCCTTACAGGTTTCTGATGTCGACAGTGGAAACTCTCGGGCACCCAGGCGGAGAAGATTGGCTTGA

Protein sequence

MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRRQVQIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALEDEKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVEDMIRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSRGIRREQMSLPPHDEVGDLAHADVSGIETGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGLLYTDPLIPRIQEQPPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSRAPRRRRLA
BLAST of Cp4.1LG03g04020 vs. Swiss-Prot
Match: RNF5_HUMAN (E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens GN=RNF5 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.4e-21
Identity = 42/84 (50.00%), Postives = 58/84 (69.05%), Query Frame = 1

Query: 137 GSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVCKGEVTTKNVTPIYG 196
           G+ F+CNICL+ AR+ VV+ CGHLYCWPCL++WL    + +ECPVCK  ++ + V P+YG
Sbjct: 22  GATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYG 81

Query: 197 RGGNTPVTEEDATLKIPLRPHARK 221
           RG   P   +D  LK P RP  ++
Sbjct: 82  RGSQKP---QDPRLKTPPRPQGQR 102

BLAST of Cp4.1LG03g04020 vs. Swiss-Prot
Match: RNF5_RAT (E3 ubiquitin-protein ligase RNF5 OS=Rattus norvegicus GN=Rnf5 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.4e-21
Identity = 45/91 (49.45%), Postives = 61/91 (67.03%), Query Frame = 1

Query: 130 EKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVCKGEVTTK 189
           E+G  S  + F+CNICL+ AR+ VV+ CGHLYCWPCL++WL    D +ECPVCK  ++ +
Sbjct: 17  ERGGAS--ATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPDRQECPVCKAGISRE 76

Query: 190 NVTPIYGRGGNTPVTEEDATLKIPLRPHARK 221
            V P+YGRG   P   +D  LK P RP  ++
Sbjct: 77  KVVPLYGRGSQKP---QDPRLKTPPRPQGQR 102

BLAST of Cp4.1LG03g04020 vs. Swiss-Prot
Match: RNF5_MOUSE (E3 ubiquitin-protein ligase RNF5 OS=Mus musculus GN=Rnf5 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.4e-21
Identity = 45/91 (49.45%), Postives = 61/91 (67.03%), Query Frame = 1

Query: 130 EKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVCKGEVTTK 189
           E+G  S  + F+CNICL+ AR+ VV+ CGHLYCWPCL++WL    D +ECPVCK  ++ +
Sbjct: 17  ERGGAS--ATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPDRQECPVCKAGISRE 76

Query: 190 NVTPIYGRGGNTPVTEEDATLKIPLRPHARK 221
            V P+YGRG   P   +D  LK P RP  ++
Sbjct: 77  KVVPLYGRGSQKP---QDPRLKTPPRPQGQR 102

BLAST of Cp4.1LG03g04020 vs. Swiss-Prot
Match: RMA3_ARATH (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana GN=RMA3 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 3.5e-20
Identity = 45/98 (45.92%), Postives = 62/98 (63.27%), Query Frame = 1

Query: 132 GSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDA-------KECPVCKG 191
           G  ++   FDCNICLD A DPVVT CGHL+CWPC+Y+WLH+   +         CPVCK 
Sbjct: 34  GQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKS 93

Query: 192 EVTTKNVTPIYGRGGNTPVT-----EEDA-TLKIPLRP 217
            +T  ++ P+YGRG ++P +     ++DA +  IP RP
Sbjct: 94  NITITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRP 131

BLAST of Cp4.1LG03g04020 vs. Swiss-Prot
Match: RMA1_CAPAN (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 6.0e-20
Identity = 43/89 (48.31%), Postives = 56/89 (62.92%), Query Frame = 1

Query: 140 FDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKE--------CPVCKGEVTTKNV 199
           FDCNICLD   +PV+T CGHLYCWPC+Y+W++  S + E        CPVCK EV+ K +
Sbjct: 39  FDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQQQPQCPVCKAEVSEKTL 98

Query: 200 TPIYGRGGNTPVTEE----DATLKIPLRP 217
            P+YGRGG +    E    +  + IP RP
Sbjct: 99  IPLYGRGGQSTKPSEGKAPNLGIVIPQRP 127

BLAST of Cp4.1LG03g04020 vs. TrEMBL
Match: A0A0A0LB96_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G806220 PE=4 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 4.7e-221
Identity = 396/426 (92.96%), Postives = 405/426 (95.07%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRR 60
           MGDEIFDAMNLDLNLGPIPEPLSNSV   EDVNLADLTE+ VHRFGE IRFRTRQRWRRR
Sbjct: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVPN-EDVNLADLTEESVHRFGETIRFRTRQRWRRR 60

Query: 61  QVQIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALED 120
           QVQIP ETQNITMELSEI+VNSGNGN+LQPLQ GEGSITAEERKNE GKTCEINNEALED
Sbjct: 61  QVQIPPETQNITMELSEIIVNSGNGNSLQPLQPGEGSITAEERKNETGKTCEINNEALED 120

Query: 121 EKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECP 180
            KTENKSDVEKGSDSDGSFFDCNICLDL+RDPVVTCCGHLYCWPCLYRWLHLHSDAKECP
Sbjct: 121 GKTENKSDVEKGSDSDGSFFDCNICLDLSRDPVVTCCGHLYCWPCLYRWLHLHSDAKECP 180

Query: 181 VCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVEDM 240
           VCKGEVTTKNVTPIYGRG NTPVTEEDATLKIP RPHARKVESLRQTIQRT  SFPVEDM
Sbjct: 181 VCKGEVTTKNVTPIYGRGSNTPVTEEDATLKIPPRPHARKVESLRQTIQRTPFSFPVEDM 240

Query: 241 IRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSRGIRREQMSLPPHDEVGDLA 300
           IRRLGNRFDFNRDLDQP EPDSS+ETFGRSPTLLNR+LTSRGIRRE++SL PHD+VGDLA
Sbjct: 241 IRRLGNRFDFNRDLDQPPEPDSSNETFGRSPTLLNRILTSRGIRRERISL-PHDDVGDLA 300

Query: 301 HADVSGIETGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGLLYTDPLIPRIQEQP 360
           H DVS IE GETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDG LYTDPLIPR QEQP
Sbjct: 301 HTDVSSIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFLYTDPLIPRSQEQP 360

Query: 361 PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSRAP 420
           PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRR DALQVSDVDSGNSRAP
Sbjct: 361 PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRNDALQVSDVDSGNSRAP 420

Query: 421 RRRRLA 427
           RRRRLA
Sbjct: 421 RRRRLA 424

BLAST of Cp4.1LG03g04020 vs. TrEMBL
Match: C6TK36_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_19G148900 PE=2 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 3.9e-135
Identity = 272/448 (60.71%), Postives = 326/448 (72.77%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRR 60
           M +E  DAMNLDLNLGP PEP     +  E VNL D   +P+ R  EA+R R RQRWR R
Sbjct: 1   MEEETSDAMNLDLNLGPGPEP-ETGPMSNEAVNLDDWIGEPLQRISEAVR-RARQRWRWR 60

Query: 61  QV-------------------QIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAE 120
            +                   QIP E +NI++EL+  +VNSGNG+ LQ   AGEGS+ AE
Sbjct: 61  HLPLPHPEFHAHFPTEATRHFQIPPEARNISVELNHFLVNSGNGSALQ---AGEGSVAAE 120

Query: 121 ERKNEAGKTCEINNEALEDEKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLY 180
           ER  E  K C+  N   EDE ++ K DVEKGS +DG FFDCNICLDLARDPVVTCCGHL+
Sbjct: 121 ERMEEVPKACDNVNGVAEDETSQKKEDVEKGSGNDGDFFDCNICLDLARDPVVTCCGHLF 180

Query: 181 CWPCLYRWLHLHSDAKECPVCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKV 240
           CWPCLYRWLHLHSDAKECPVCKGEVT K+VTP+YGRG N    EED+ LKIP RP A++V
Sbjct: 181 CWPCLYRWLHLHSDAKECPVCKGEVTLKSVTPVYGRGNNVRGPEEDSALKIPPRPQAKRV 240

Query: 241 ESLRQTIQRTALSFPVEDMIRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSR 300
           ESLRQTIQR A + PVE+MIRRLG+R D  RDL QP EPD++ ET  R+ +LL+R LT+R
Sbjct: 241 ESLRQTIQRNAFALPVEEMIRRLGSRIDLTRDLVQPPEPDAARETAERTTSLLSRFLTAR 300

Query: 301 GIRREQMSL--PPHDEVGDLAHADVSGIETGE--TRLQSLPVLRSLLHRTRVSSLTSAFN 360
           G+RRE  +L  PP D +G LA  +V+  E G+  +R+QS  + R+  HR  +S+L+SA  
Sbjct: 301 GMRREHQNLVAPPEDVMG-LAQNNVNTSEAGDPRSRVQSHLLRRTQSHRATLSTLSSALT 360

Query: 361 SADRLPDGLLYTDPLIPRIQEQPPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSIST 420
           SA+RL +    ++PL  R QEQPPPPV+DRDSFSSI AVINSESQ+DTAVEIDSMVS+ST
Sbjct: 361 SAERLVEAYFRSNPL-GRNQEQPPPPVDDRDSFSSIGAVINSESQLDTAVEIDSMVSLST 420

Query: 421 SSSRRRTDALQVSDVDSGNSRAPRRRRL 426
           SSSRRR DA +VSDVDSG+SRAPRRRRL
Sbjct: 421 SSSRRRNDASRVSDVDSGDSRAPRRRRL 441

BLAST of Cp4.1LG03g04020 vs. TrEMBL
Match: A0A0B2SK57_GLYSO (E3 ubiquitin-protein ligase RMA3 OS=Glycine soja GN=glysoja_028432 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 3.9e-135
Identity = 272/448 (60.71%), Postives = 326/448 (72.77%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRR 60
           M +E  DAMNLDLNLGP PEP     +  E VNL D   +P+ R  EA+R R RQRWR R
Sbjct: 1   MEEETSDAMNLDLNLGPGPEP-ETGPMSNEAVNLDDWIGEPLQRISEAVR-RARQRWRWR 60

Query: 61  QV-------------------QIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAE 120
            +                   QIP E +NI++EL+  +VNSGNG+ LQ   AGEGS+ AE
Sbjct: 61  HLPLPHPEFHAHFPTEATRHFQIPPEARNISVELNHFLVNSGNGSALQ---AGEGSVAAE 120

Query: 121 ERKNEAGKTCEINNEALEDEKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLY 180
           ER  E  K C+  N   EDE ++ K DVEKGS +DG FFDCNICLDLARDPVVTCCGHL+
Sbjct: 121 ERMEEVPKACDNVNGVAEDETSQKKEDVEKGSGNDGDFFDCNICLDLARDPVVTCCGHLF 180

Query: 181 CWPCLYRWLHLHSDAKECPVCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKV 240
           CWPCLYRWLHLHSDAKECPVCKGEVT K+VTP+YGRG N    EED+ LKIP RP A++V
Sbjct: 181 CWPCLYRWLHLHSDAKECPVCKGEVTLKSVTPVYGRGNNVRGPEEDSALKIPPRPQAKRV 240

Query: 241 ESLRQTIQRTALSFPVEDMIRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSR 300
           ESLRQTIQR A + PVE+MIRRLG+R D  RDL QP EPD++ ET  R+ +LL+R LT+R
Sbjct: 241 ESLRQTIQRNAFALPVEEMIRRLGSRIDLTRDLVQPPEPDAARETAERTTSLLSRFLTAR 300

Query: 301 GIRREQMSL--PPHDEVGDLAHADVSGIETGE--TRLQSLPVLRSLLHRTRVSSLTSAFN 360
           G+RRE  +L  PP D +G LA  +V+  E G+  +R+QS  + R+  HR  +S+L+SA  
Sbjct: 301 GMRREHQNLVAPPEDVMG-LAQNNVNTSEAGDPRSRVQSHLLRRTQSHRATLSTLSSALT 360

Query: 361 SADRLPDGLLYTDPLIPRIQEQPPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSIST 420
           SA+RL +    ++PL  R QEQPPPPV+DRDSFSSI AVINSESQ+DTAVEIDSMVS+ST
Sbjct: 361 SAERLVEAYFRSNPL-GRNQEQPPPPVDDRDSFSSIGAVINSESQLDTAVEIDSMVSLST 420

Query: 421 SSSRRRTDALQVSDVDSGNSRAPRRRRL 426
           SSSRRR DA +VSDVDSG+SRAPRRRRL
Sbjct: 421 SSSRRRNDASRVSDVDSGDSRAPRRRRL 441

BLAST of Cp4.1LG03g04020 vs. TrEMBL
Match: V7CYG5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G143400g PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 5.1e-135
Identity = 269/446 (60.31%), Postives = 321/446 (71.97%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRR 60
           M +E  DAMNLDLNLGP PEP +  +   E VNL D   +P+ R  EA+RFR RQRWR R
Sbjct: 1   MEEETSDAMNLDLNLGPGPEPETGPISN-EAVNLDDWIGEPLQRISEAVRFRARQRWRWR 60

Query: 61  QV-------------------QIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAE 120
            +                    IP E  NI+MEL++ +VNSGNG  LQ   AGEGS+ AE
Sbjct: 61  HLPLPHPELHVHIPTEAARHFHIPPEAHNISMELNQFLVNSGNGTALQ---AGEGSVAAE 120

Query: 121 ERKNEAGKTCEINNEALEDEKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLY 180
           ER  EA K CE  N   EDE ++ K DVEKGS +DG FFDCNICLD++RDPVVTCCGHL+
Sbjct: 121 ERMEEAPKACENVNGVAEDEASQKKDDVEKGSGTDGDFFDCNICLDISRDPVVTCCGHLF 180

Query: 181 CWPCLYRWLHLHSDAKECPVCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKV 240
           CWPCLYRWLHLHSDAKECPVCKGEVT K+VTPIYGRG N    EED+ LKIP RP AR+V
Sbjct: 181 CWPCLYRWLHLHSDAKECPVCKGEVTLKSVTPIYGRGNNVRGHEEDSALKIPPRPQARRV 240

Query: 241 ESLRQTIQRTALSFPVEDMIRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSR 300
           ESLRQTIQR A + PVE+MIRRLG+R D  RD  QP E D + ET  R+ +LL+R LT+R
Sbjct: 241 ESLRQTIQRNAFALPVEEMIRRLGSRIDLTRDFVQPHEVDGARETAERTTSLLSRFLTAR 300

Query: 301 GIRREQMSLPPHDEVGDLAHADVSGIETGE--TRLQSLPVLRSLLHRTRVSSLTSAFNSA 360
           G+RREQ   P  ++V  LA  + +  E G+  +R+QS  + R+  HR  +S+L+SA  SA
Sbjct: 301 GMRREQNLGPLPEDVMALAQNNANTAEAGDNRSRVQSHLLRRTQSHRATLSTLSSALTSA 360

Query: 361 DRLPDGLLYTDPLIPRIQEQPPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSS 420
           +RL +    ++P I R QEQPPPPV+DRDSFSSI AVINSESQ+DTAVEIDSMVS+STSS
Sbjct: 361 ERLVEAYFRSNP-IGRNQEQPPPPVDDRDSFSSIGAVINSESQVDTAVEIDSMVSLSTSS 420

Query: 421 SRRRTDALQVSDVDSGNSRAPRRRRL 426
           SRRR DA ++SDVDSG+SRAPRRRRL
Sbjct: 421 SRRRNDASRLSDVDSGDSRAPRRRRL 441

BLAST of Cp4.1LG03g04020 vs. TrEMBL
Match: A0A0B2PDJ6_GLYSO (E3 ubiquitin-protein ligase RMA3 OS=Glycine soja GN=glysoja_012739 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 5.1e-135
Identity = 273/448 (60.94%), Postives = 325/448 (72.54%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRR 60
           M +E  DAMNLDLNLGP PEP +  +   E VNL D   +P+ R  EA+R+R RQRWR R
Sbjct: 1   MEEETSDAMNLDLNLGPGPEPETGPISN-EAVNLDDWIGEPLQRISEAVRYRARQRWRWR 60

Query: 61  QV-------------------QIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAE 120
           Q+                   QIP E  NI++EL+  +VNSGNG+ LQ   AGEGS+ AE
Sbjct: 61  QLPLPPPEFHAHFPTEATRHFQIPPEAHNISVELNHFLVNSGNGSALQ---AGEGSVAAE 120

Query: 121 ERKNEAGKTCEINNEALEDEKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLY 180
           ER  E  K CE  N   EDE ++ K D+EKGS +DG FFDCNICLDLARDPVVTCCGHL+
Sbjct: 121 ERMEEVPKACENINGVAEDEASQKKGDIEKGSGNDGDFFDCNICLDLARDPVVTCCGHLF 180

Query: 181 CWPCLYRWLHLHSDAKECPVCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKV 240
           CW CLYRWLHLHSDAKECPVCKGEVT K+VTPIYGR  N    EED+ LKIP RP A++V
Sbjct: 181 CWSCLYRWLHLHSDAKECPVCKGEVTLKSVTPIYGRANNVRGPEEDSALKIPPRPQAKRV 240

Query: 241 ESLRQTIQRTALSFPVEDMIRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSR 300
           ESLRQTIQR A + PVE+MIRRLG+R +  RDL QP EPD++ ET  R+ +LL R LT++
Sbjct: 241 ESLRQTIQRNAFALPVEEMIRRLGSRIELTRDLVQPPEPDAARETAERTTSLL-RFLTAQ 300

Query: 301 GIRREQMSL--PPHDEVGDLAHADVSGIETGE--TRLQSLPVLRSLLHRTRVSSLTSAFN 360
           GIRRE  +L  PP D +G LA  +V+  E G+  TR+QS  + R+  HR  +S+L+SA  
Sbjct: 301 GIRREHQNLVAPPEDVMG-LAQNNVNTSEAGDPRTRVQSHLLRRTQSHRATLSTLSSALT 360

Query: 361 SADRLPDGLLYTDPLIPRIQEQPPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSIST 420
           SA+RL +    ++PL  R QEQPPPPVEDRDSFSSI AVINSESQ+DTAVEIDSMVS+ST
Sbjct: 361 SAERLVEAYFRSNPL-GRNQEQPPPPVEDRDSFSSIGAVINSESQLDTAVEIDSMVSLST 420

Query: 421 SSSRRRTDALQVSDVDSGNSRAPRRRRL 426
           SSSRRR DA +VSDVDSG+SRAPRRRRL
Sbjct: 421 SSSRRRNDASRVSDVDSGDSRAPRRRRL 441

BLAST of Cp4.1LG03g04020 vs. TAIR10
Match: AT3G58030.1 (AT3G58030.1 RING/U-box superfamily protein)

HSP 1 Score: 374.4 bits (960), Expect = 9.3e-104
Identity = 240/452 (53.10%), Postives = 293/452 (64.82%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPE----PLSNSVLQIEDVNLADLTEDPVHRFGEAI-RFRTRQ 60
           MG+E+ D MNLDLNLGP PE    P  N     E VNLAD T DP  R  EA+ R RTR 
Sbjct: 1   MGEELADTMNLDLNLGPGPESDLQPAPN-----ETVNLADWTNDPPERSSEAVTRIRTRH 60

Query: 61  RWRRRQVQIP----SETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTC 120
           R R RQ+ +P    SET  + +EL+++M NS N      +Q GEGS    ER NE  K C
Sbjct: 61  RTRFRQLNLPIPVLSETHTMAIELNQLMGNSVN---RAAMQTGEGS----ERGNEDLKMC 120

Query: 121 EINNEALEDEKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLH 180
           E  + AL D   + K+DVEK S SDG+FFDCNICLDL+++PV+TCCGHLYCWPCLY+WL 
Sbjct: 121 ENGDGALGDGVLDKKADVEKSSGSDGNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQ 180

Query: 181 LHSDAKECPVCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRT 240
           + SDAKECPVCKGEVT+K VTPIYGRG +    EE    K+P+RPHAR++ESLR TIQR+
Sbjct: 181 I-SDAKECPVCKGEVTSKTVTPIYGRGNHKREIEESLDTKVPMRPHARRIESLRNTIQRS 240

Query: 241 ALSFPVEDMIRRLGNRFDFNRDLDQPLEPD-----SSHETFGRSPTLLNRLLTSRGIRRE 300
             + P+E+MIRR+ NRF    D D    PD     +S     R+ ++LNRL+TSRG+R E
Sbjct: 241 PFTIPMEEMIRRIQNRF----DRDSTPVPDFSNREASERVNDRANSILNRLMTSRGVRSE 300

Query: 301 Q-------MSLPPHDEVGDLAHADVSGIE-TGETRLQSLPVLRSL-LHR-TRVSSLTSAF 360
           Q        ++    E  DL       +E    TR   L + R L  HR  R+S+ TSA 
Sbjct: 301 QNQASAAAAAIVAASEDIDLNPNIAPDLEGESNTRFHPLLIRRQLQSHRVARISTFTSAL 360

Query: 361 NSADRLPDGLLYTDPLIPRIQEQ---PPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMV 420
           +SA+RL D    T PL    QEQ    P  V+DRDSFSSIAAVINSESQ+DTAVEIDSM 
Sbjct: 361 SSAERLVDAYFRTHPLGRNHQEQNHHAPVVVDDRDSFSSIAAVINSESQVDTAVEIDSM- 420

Query: 421 SISTSSSRRRTD-ALQVSDVDSGNSRAPRRRR 425
           ++STSSSRRR +   +VSDVDS +SR PRRRR
Sbjct: 421 ALSTSSSRRRNENGSRVSDVDSADSRPPRRRR 434

BLAST of Cp4.1LG03g04020 vs. TAIR10
Match: AT2G42030.1 (AT2G42030.1 RING/U-box superfamily protein)

HSP 1 Score: 294.3 bits (752), Expect = 1.2e-79
Identity = 209/447 (46.76%), Postives = 266/447 (59.51%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPE----PLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQR 60
           MG+EI   +NLDLNLGP PE    P +N         LA    + + RF    R R R R
Sbjct: 1   MGEEISSTVNLDLNLGPDPELGLEPATNYARSDWGNGLAARESESLRRF----RSRYRSR 60

Query: 61  WRR-RQVQIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINN 120
           +RR   + + +ET +  MELS++++ SGN   +  L AGEGS+ + ER  E  K CE  +
Sbjct: 61  FRRIDMLPVLAETHSPAMELSQLLITSGN---VVALPAGEGSMASGERVIEDSKKCENGS 120

Query: 121 EAL-EDEKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHS 180
           + + ED  TE K DVEK   SDGSFFDC ICLDL++DPVVT CGHLYCW CLY+WL + S
Sbjct: 121 KVMAEDNVTEEKRDVEKSVGSDGSFFDCYICLDLSKDPVVTNCGHLYCWSCLYQWLQV-S 180

Query: 181 DAKECPVCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALS 240
           +AKECPVCKGEV+ K VTPIYGRG     +EE +  KIP RP AR+ ESLR T+ R+   
Sbjct: 181 EAKECPVCKGEVSVKTVTPIYGRGIQKRESEEVSNTKIPSRPQARRTESLRTTLNRSG-H 240

Query: 241 FPVEDMIRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSRGIRREQ------M 300
            P E             R L   LE +SS       P+ LNR +TSRG+R E+      +
Sbjct: 241 IPTE-----------IFRHLQDRLERESSTGERRARPS-LNRFMTSRGVRAEENQSSEAL 300

Query: 301 SLPPHDEVGDLAHADVSGIETGETRLQSLPVLRSLLHRT-------RVSSLTSAFNSADR 360
             P  DE+ D+    ++     E   ++L   R+L  +        RVSS T   +SA+R
Sbjct: 301 VEPSSDEINDI-DLILNNAPENEEENENLSSSRALATQRRWAQMYGRVSSFT--LSSAER 360

Query: 361 LPDGLLYTDPLIPRIQEQ---PPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTS 420
           L D  L T  L  R QEQ   PP  VEDRDSFSSI  VINSESQ++TA EI+SM+++STS
Sbjct: 361 LADTYLITHAL-GRNQEQNSSPPVGVEDRDSFSSIVGVINSESQVETAAEINSMLTVSTS 420

Query: 421 SS-RRRTDALQVSDVDSGNSRAPRRRR 425
           SS RR  ++ +VSDVDS +SR  RRRR
Sbjct: 421 SSVRRHENSSRVSDVDSADSRPLRRRR 422

BLAST of Cp4.1LG03g04020 vs. TAIR10
Match: AT1G19310.1 (AT1G19310.1 RING/U-box superfamily protein)

HSP 1 Score: 119.4 bits (298), Expect = 5.4e-27
Identity = 48/93 (51.61%), Postives = 70/93 (75.27%), Query Frame = 1

Query: 133 SDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVCKGEVTTKNVT 192
           +++D S F+CNICLDLA+DP+VT CGHL+CWPCLY+WLHLHS +K+CPVCK  +    + 
Sbjct: 14  NNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKDCPVCKAVIEEDRLV 73

Query: 193 PIYGRGGNT--PVTEEDATLKIPLRPHARKVES 224
           P+YGRG ++  P ++    L++P RP  ++ E+
Sbjct: 74  PLYGRGKSSADPRSKSIPGLEVPNRPSGQRPET 106

BLAST of Cp4.1LG03g04020 vs. TAIR10
Match: AT2G23780.1 (AT2G23780.1 RING/U-box superfamily protein)

HSP 1 Score: 115.9 bits (289), Expect = 6.0e-26
Identity = 54/118 (45.76%), Postives = 73/118 (61.86%), Query Frame = 1

Query: 119 EDEKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKE 178
           E   + + SD    ++  G  F+CNIC +LA+DP+VT CGHL+CWPCLYRWLH HS ++E
Sbjct: 5   ESSTSTSYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLHHHSHSQE 64

Query: 179 CPVCKGEVTTKNVTPIYGRGGN--TPVTEEDATLKIPLRPHARKVESLRQTIQRTALS 235
           CPVCK  V    + P+YGRG N   P ++    L+IP RP  ++ E+     Q  A S
Sbjct: 65  CPVCKAVVQDDKLVPLYGRGKNQTDPRSKRYPGLRIPNRPTGQRPETAAPPPQPEAAS 122

BLAST of Cp4.1LG03g04020 vs. TAIR10
Match: AT2G44410.1 (AT2G44410.1 RING/U-box superfamily protein)

HSP 1 Score: 115.5 bits (288), Expect = 7.8e-26
Identity = 110/360 (30.56%), Postives = 157/360 (43.61%), Query Frame = 1

Query: 101 EERKNEAGKTCEINNEALEDEKTENKS-------DVEKGSDSDGSFFDCNICLDLARDPV 160
           E R + AG   E + E L  E  ENK+       ++EK S   G FFDCNICL+ A DP+
Sbjct: 78  EHRDSTAGVIHERSRERLV-ENGENKTYLIAKALNMEKTSSVPGGFFDCNICLEKAEDPI 137

Query: 161 VTCCGHLYCWPCLYRWLHLHSDAKECPVCKGEVTTKNVTPIYGRGGNTPVTE---EDATL 220
           +TCCGHL+CW C Y+   ++ + KECPVC GEVT   V PIYG G +   T+   ED  +
Sbjct: 138 LTCCGHLFCWGCFYQLPLIYLNIKECPVCDGEVTDAEVIPIYGNGDDCDGTKPKLEDCGI 197

Query: 221 KIPLRPHARKVESLRQ-TIQRTALSFP------VEDMIRRL----------GNRFDFNRD 280
            +P RP+A++VES+RQ  I R    FP      +E + R +          G+ F     
Sbjct: 198 SLPPRPNAKRVESVRQKIINRGNPFFPGHENEAIEHIRRTIDSIGLQALAQGDEFGLTNI 257

Query: 281 LDQ-----PLEPDSSHETFGRSP----TLLNRLLTSRGIRREQMSLP-PHDEVGDLAHAD 340
           ++        +    H      P     LL    T  G+  +   +P P D+      + 
Sbjct: 258 INNGGDNGGQQQQQQHHHHHHPPFGPLRLLPPYATFPGLLVDTSDIPAPFDDDAFDVDSF 317

Query: 341 VSGIETGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGLLYTDPLIPRIQEQPPPP 400
           V        R +  P +R+   R R +      N++  +   L  +    PR    P   
Sbjct: 318 VDTTSLRRNRRRPSPAVRASYQRNRSN------NASQTISFRLGSSASSAPREFAVPSSS 377

Query: 401 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSRAPRRR 424
           +  R    +   V+ S              S S SSSRRRT+     DV++G     RRR
Sbjct: 378 IMTRSQTVNPTEVVTS--------------STSASSSRRRTE-----DVNNGPRTRSRRR 411

BLAST of Cp4.1LG03g04020 vs. NCBI nr
Match: gi|659084718|ref|XP_008443034.1| (PREDICTED: uncharacterized protein LOC103486753 [Cucumis melo])

HSP 1 Score: 785.4 bits (2027), Expect = 5.0e-224
Identity = 400/426 (93.90%), Postives = 408/426 (95.77%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRR 60
           MGDE+FDAMNLDLNLGPIPEPLSNSV   EDVNLADLTE+PVHRFGE IRFRTRQRWRRR
Sbjct: 1   MGDEMFDAMNLDLNLGPIPEPLSNSVPN-EDVNLADLTEEPVHRFGETIRFRTRQRWRRR 60

Query: 61  QVQIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALED 120
           QVQIP ETQNITMELSEIMVNSGNGN+LQPLQ GEGSITAEERKNE GKTCEINNEALED
Sbjct: 61  QVQIPPETQNITMELSEIMVNSGNGNSLQPLQPGEGSITAEERKNETGKTCEINNEALED 120

Query: 121 EKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECP 180
           EKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECP
Sbjct: 121 EKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECP 180

Query: 181 VCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVEDM 240
           VCKGEVTTKNVTPIYGRG NTP TEEDATLKIPLRPHARKVESLRQTIQRTA SFPVEDM
Sbjct: 181 VCKGEVTTKNVTPIYGRGSNTPATEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEDM 240

Query: 241 IRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSRGIRREQMSLPPHDEVGDLA 300
           IRRLGNRFDFNRDLDQP EPDSS+ETFGRSPTLLNR+LTSRGIRREQ+SL PHD+VGDLA
Sbjct: 241 IRRLGNRFDFNRDLDQPPEPDSSNETFGRSPTLLNRILTSRGIRREQISL-PHDDVGDLA 300

Query: 301 HADVSGIETGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGLLYTDPLIPRIQEQP 360
           H DVS IETGETRLQSLPVLRSLLHRTRVSSL SAFNSADRLP+G LYTDPLIPR QEQP
Sbjct: 301 HTDVSSIETGETRLQSLPVLRSLLHRTRVSSLASAFNSADRLPEGFLYTDPLIPRSQEQP 360

Query: 361 PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSRAP 420
           PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRR DALQVSDVDSGNSRAP
Sbjct: 361 PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRNDALQVSDVDSGNSRAP 420

Query: 421 RRRRLA 427
           RRRRLA
Sbjct: 421 RRRRLA 424

BLAST of Cp4.1LG03g04020 vs. NCBI nr
Match: gi|778684736|ref|XP_011652084.1| (PREDICTED: uncharacterized protein LOC101220497 [Cucumis sativus])

HSP 1 Score: 775.0 bits (2000), Expect = 6.7e-221
Identity = 396/426 (92.96%), Postives = 405/426 (95.07%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRR 60
           MGDEIFDAMNLDLNLGPIPEPLSNSV   EDVNLADLTE+ VHRFGE IRFRTRQRWRRR
Sbjct: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVPN-EDVNLADLTEESVHRFGETIRFRTRQRWRRR 60

Query: 61  QVQIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALED 120
           QVQIP ETQNITMELSEI+VNSGNGN+LQPLQ GEGSITAEERKNE GKTCEINNEALED
Sbjct: 61  QVQIPPETQNITMELSEIIVNSGNGNSLQPLQPGEGSITAEERKNETGKTCEINNEALED 120

Query: 121 EKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECP 180
            KTENKSDVEKGSDSDGSFFDCNICLDL+RDPVVTCCGHLYCWPCLYRWLHLHSDAKECP
Sbjct: 121 GKTENKSDVEKGSDSDGSFFDCNICLDLSRDPVVTCCGHLYCWPCLYRWLHLHSDAKECP 180

Query: 181 VCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVEDM 240
           VCKGEVTTKNVTPIYGRG NTPVTEEDATLKIP RPHARKVESLRQTIQRT  SFPVEDM
Sbjct: 181 VCKGEVTTKNVTPIYGRGSNTPVTEEDATLKIPPRPHARKVESLRQTIQRTPFSFPVEDM 240

Query: 241 IRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSRGIRREQMSLPPHDEVGDLA 300
           IRRLGNRFDFNRDLDQP EPDSS+ETFGRSPTLLNR+LTSRGIRRE++SL PHD+VGDLA
Sbjct: 241 IRRLGNRFDFNRDLDQPPEPDSSNETFGRSPTLLNRILTSRGIRRERISL-PHDDVGDLA 300

Query: 301 HADVSGIETGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGLLYTDPLIPRIQEQP 360
           H DVS IE GETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDG LYTDPLIPR QEQP
Sbjct: 301 HTDVSSIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFLYTDPLIPRSQEQP 360

Query: 361 PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSRAP 420
           PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRR DALQVSDVDSGNSRAP
Sbjct: 361 PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRNDALQVSDVDSGNSRAP 420

Query: 421 RRRRLA 427
           RRRRLA
Sbjct: 421 RRRRLA 424

BLAST of Cp4.1LG03g04020 vs. NCBI nr
Match: gi|645271442|ref|XP_008240913.1| (PREDICTED: uncharacterized protein LOC103339404 [Prunus mume])

HSP 1 Score: 502.3 bits (1292), Expect = 8.4e-139
Identity = 278/432 (64.35%), Postives = 327/432 (75.69%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQ-RWRR 60
           MG+E  DAMNLDLNLGP PEP S S    E VNL    E P+HR  E +  R R  RW  
Sbjct: 1   MGEETSDAMNLDLNLGPGPEPASASN---EPVNLDAWIEAPIHRIAEPVGIRARHWRWAH 60

Query: 61  RQVQIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALE 120
                  E QNI+MEL+++MVNSGN +TLQ   AGEGS+TAEER ++  K+CE NN   E
Sbjct: 61  ------PEGQNISMELNQLMVNSGNSSTLQ---AGEGSVTAEERTSDVPKSCENNNGVSE 120

Query: 121 DEKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKEC 180
           +E ++NK DVEKGS +DGSFFDCNICLDLARDPVVTCCGHL+CWPCLYRWLH+HSDAKEC
Sbjct: 121 NETSDNKDDVEKGSGNDGSFFDCNICLDLARDPVVTCCGHLFCWPCLYRWLHVHSDAKEC 180

Query: 181 PVCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVED 240
           PVCKGEVT KNVTPIYG  GNT + E D   KIPLRP AR+VESLRQTIQR+A +FPVE+
Sbjct: 181 PVCKGEVTIKNVTPIYGH-GNT-MREPDDDSKIPLRPQARRVESLRQTIQRSAFNFPVEE 240

Query: 241 MIRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSRGIRREQMSLPPHDEVGDL 300
           MIRRLGNRFD  RD+ QPLEP+++ ET  R+ TLLNR+LTSRG+RREQ  +   D+V DL
Sbjct: 241 MIRRLGNRFDLTRDIVQPLEPENARETAERTSTLLNRILTSRGLRREQNPVALADDVVDL 300

Query: 301 AHADVSGIETGET-RLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGLLYTDPLI----- 360
               ++G+ETGE  RLQSL + RS  HR   SS +SA +SA+RL +    + P       
Sbjct: 301 TQGSMTGLETGENRRLQSLLLRRSQSHRAAFSSFSSALSSAERLVEAYFRSHPTSTGRNQ 360

Query: 361 PRIQEQPPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVD 420
            + Q+Q P PV+DRDSFSSIAAVINSESQ+DTAVEIDSMVS+STSSSRRR DA +VSD+D
Sbjct: 361 EQQQQQQPAPVDDRDSFSSIAAVINSESQLDTAVEIDSMVSLSTSSSRRRNDASRVSDMD 418

Query: 421 SGNSRAPRRRRL 426
           SG+SRAPRRRRL
Sbjct: 421 SGDSRAPRRRRL 418

BLAST of Cp4.1LG03g04020 vs. NCBI nr
Match: gi|694314042|ref|XP_009369445.1| (PREDICTED: RING finger protein 219 [Pyrus x bretschneideri])

HSP 1 Score: 492.7 bits (1267), Expect = 6.7e-136
Identity = 270/427 (63.23%), Postives = 327/427 (76.58%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRR 60
           MG+E  DAMNLDLNL P P+    S+L  E VNL    + P HR  EA+R        R+
Sbjct: 1   MGEETSDAMNLDLNLAPGPDV--GSMLN-EPVNLDTWIDSPFHRIAEAVR--------RQ 60

Query: 61  QVQIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALED 120
               P E +NI+MEL+E+MVNSGN +TLQP   GEGS+TAEER ++A KTCE NN  LE+
Sbjct: 61  WTWSPPEGRNISMELNELMVNSGNSSTLQP---GEGSVTAEERTSDASKTCENNNGILEN 120

Query: 121 EKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECP 180
           E + NK DVEKG  SDGSFFDCNICLDLARDP+VTCCGHL+CWPC+YRWLH+  DAKECP
Sbjct: 121 ENSVNKDDVEKGGGSDGSFFDCNICLDLARDPIVTCCGHLFCWPCIYRWLHV-QDAKECP 180

Query: 181 VCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVEDM 240
           VCKGEVT KNVTPIYGRG N    ++D+ LKIPLRPHAR+VESLRQ+IQR+AL+FPVE+M
Sbjct: 181 VCKGEVTMKNVTPIYGRGNNVREPDDDSNLKIPLRPHARRVESLRQSIQRSALTFPVEEM 240

Query: 241 IRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSRGIRREQMSLPPHDEVGDLA 300
           IRRLGNRFD  RD+  P EP+++ ET  R+  LLNR+LTSRG+RREQ  +   D+V D+ 
Sbjct: 241 IRRLGNRFDLTRDVIHPSEPENARETAERA-NLLNRILTSRGLRREQNPVAVADDVVDIT 300

Query: 301 HADVSGIETGET-RLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGLLYTDPLIP-RIQE 360
            +++SG+ET E  +LQSL + R+  HR  +SSL+SA +SA+RL +      P  P R QE
Sbjct: 301 QSNMSGLETRENHQLQSLLLRRTQSHRATLSSLSSALSSAERLVESFYRNHPTPPGRNQE 360

Query: 361 QPPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSR 420
           Q P PV+DRDSFSSIAAVINSESQ+DTAVEIDSMVS+STSSSRRR D+ +VSD+DSG+SR
Sbjct: 361 QQPAPVDDRDSFSSIAAVINSESQLDTAVEIDSMVSLSTSSSRRRNDSSRVSDMDSGDSR 411

Query: 421 APRRRRL 426
           A RRRRL
Sbjct: 421 AHRRRRL 411

BLAST of Cp4.1LG03g04020 vs. NCBI nr
Match: gi|657990000|ref|XP_008387222.1| (PREDICTED: uncharacterized protein LOC103449673 [Malus domestica])

HSP 1 Score: 490.7 bits (1262), Expect = 2.5e-135
Identity = 267/427 (62.53%), Postives = 326/427 (76.35%), Query Frame = 1

Query: 1   MGDEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRR 60
           MG+E  DAMNLDLNL P P+    S+L  E VNL    + P+HR  EA+R        R+
Sbjct: 1   MGEETSDAMNLDLNLAPSPD--LGSMLN-EPVNLDTWIDSPIHRIAEAVR--------RQ 60

Query: 61  QVQIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALED 120
               P E +N++MEL+E+MVNSGN +TLQP   GEGS+TAEER ++A KTCE NN  LE+
Sbjct: 61  WTWSPPEGRNVSMELNELMVNSGNSSTLQP---GEGSVTAEERTSDASKTCENNNGILEN 120

Query: 121 EKTENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECP 180
           E + NK DVEKG  SDGSFFDCNICLDLARDP+VTCCGHL+CWPC+YRWLH+  DAKECP
Sbjct: 121 ENSVNKDDVEKGGGSDGSFFDCNICLDLARDPIVTCCGHLFCWPCIYRWLHV-QDAKECP 180

Query: 181 VCKGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVEDM 240
           VCKGEVT KNVTPIYGRG N    ++D+ LKIPLRPHAR+VESLRQTIQR A++FPVE+M
Sbjct: 181 VCKGEVTMKNVTPIYGRGNNVREPDDDSNLKIPLRPHARRVESLRQTIQRXAITFPVEEM 240

Query: 241 IRRLGNRFDFNRDLDQPLEPDSSHETFGRSPTLLNRLLTSRGIRREQMSLPPHDEVGDLA 300
           IRRLGNRFD   D+  P EP+++ ET  R+  LLNR+LTSRG+RREQ  +   D+V D+ 
Sbjct: 241 IRRLGNRFDLTXDVIHPSEPENARETAERA-NLLNRILTSRGLRREQNPVALADDVVDIT 300

Query: 301 HADVSGIETGET-RLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGLLYTDPLIP-RIQE 360
            ++++G+ET E  +LQSL + R+  HR  +SSL+SA +SA+RL +      P  P R QE
Sbjct: 301 QSNMTGLETRENHQLQSLLLRRTQSHRATLSSLSSALSSAERLVESFYRNHPTPPGRNQE 360

Query: 361 QPPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSR 420
           Q P PV+DRDSFSSIAAVINSESQ+DTAVEIDSMVS+STSSSRRR D+ +VSD+DSG+SR
Sbjct: 361 QQPAPVDDRDSFSSIAAVINSESQLDTAVEIDSMVSLSTSSSRRRNDSSRVSDMDSGDSR 411

Query: 421 APRRRRL 426
           A RRRRL
Sbjct: 421 AHRRRRL 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RNF5_HUMAN1.4e-2150.00E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens GN=RNF5 PE=1 SV=1[more]
RNF5_RAT2.4e-2149.45E3 ubiquitin-protein ligase RNF5 OS=Rattus norvegicus GN=Rnf5 PE=2 SV=1[more]
RNF5_MOUSE2.4e-2149.45E3 ubiquitin-protein ligase RNF5 OS=Mus musculus GN=Rnf5 PE=1 SV=1[more]
RMA3_ARATH3.5e-2045.92E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana GN=RMA3 PE=1 SV=1[more]
RMA1_CAPAN6.0e-2048.31E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum GN=RMA1H1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LB96_CUCSA4.7e-22192.96Uncharacterized protein OS=Cucumis sativus GN=Csa_3G806220 PE=4 SV=1[more]
C6TK36_SOYBN3.9e-13560.71Uncharacterized protein OS=Glycine max GN=GLYMA_19G148900 PE=2 SV=1[more]
A0A0B2SK57_GLYSO3.9e-13560.71E3 ubiquitin-protein ligase RMA3 OS=Glycine soja GN=glysoja_028432 PE=4 SV=1[more]
V7CYG5_PHAVU5.1e-13560.31Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G143400g PE=4 SV=1[more]
A0A0B2PDJ6_GLYSO5.1e-13560.94E3 ubiquitin-protein ligase RMA3 OS=Glycine soja GN=glysoja_012739 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G58030.19.3e-10453.10 RING/U-box superfamily protein[more]
AT2G42030.11.2e-7946.76 RING/U-box superfamily protein[more]
AT1G19310.15.4e-2751.61 RING/U-box superfamily protein[more]
AT2G23780.16.0e-2645.76 RING/U-box superfamily protein[more]
AT2G44410.17.8e-2630.56 RING/U-box superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659084718|ref|XP_008443034.1|5.0e-22493.90PREDICTED: uncharacterized protein LOC103486753 [Cucumis melo][more]
gi|778684736|ref|XP_011652084.1|6.7e-22192.96PREDICTED: uncharacterized protein LOC101220497 [Cucumis sativus][more]
gi|645271442|ref|XP_008240913.1|8.4e-13964.35PREDICTED: uncharacterized protein LOC103339404 [Prunus mume][more]
gi|694314042|ref|XP_009369445.1|6.7e-13663.23PREDICTED: RING finger protein 219 [Pyrus x bretschneideri][more]
gi|657990000|ref|XP_008387222.1|2.5e-13562.53PREDICTED: uncharacterized protein LOC103449673 [Malus domestica][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR017907Znf_RING_CS
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g04020.1Cp4.1LG03g04020.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 142..182
score: 7.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 142..183
score: 13
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 124..195
score: 1.1
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 157..166
scor
NoneNo IPR availablePANTHERPTHR12313RNF5coord: 18..249
score: 3.3E
NoneNo IPR availablePANTHERPTHR12313:SF14E3 UBIQUITIN-PROTEIN LIGASE RMA1-RELATEDcoord: 18..249
score: 3.3E
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 142..187
score: 5.
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 138..195
score: 6.86