Cp4.1LG01g16960 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG01g16960
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCalcium-binding EF-hand family protein, putative
LocationCp4.1LG01 : 11018886 .. 11019344 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTATCTCCAAACTTCAAGCTTCCATGGCTAAGTACGAAGCAAGTGAGATGATCAAGAAGCATATTAGAAATGGTGATAATGTTCAACTACAATCTTTCAGTGGCATTGCTCGAGATTCAACTCAACTCAACAATCAAGGCAAGATACTAGAAATCCTAAAAGATAACAAGAATGTTGGGCTCAAGCTTAGTTCAAATGCTCACCCAAAGACATCTAAGTTGCAATTTAGTCGAGAGCAGATGAAGGAGATCTTTAGATATCATGATAACGATAAGGATGGTTTTCTCAATTTAAGGGAGCTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGGTATGTCATATGCGGATGCAAATAAAGATGGCCTTATCAGCGAGGCTGAACTTGACAAACTCATTGATTATGCTCAGAAATTTGTGAAGAAGAAATGA

mRNA sequence

ATGTTTATCTCCAAACTTCAAGCTTCCATGGCTAAGTACGAAGCAAGTGAGATGATCAAGAAGCATATTAGAAATGGTGATAATGTTCAACTACAATCTTTCAGTGGCATTGCTCGAGATTCAACTCAACTCAACAATCAAGGCAAGATACTAGAAATCCTAAAAGATAACAAGAATGTTGGGCTCAAGCTTAGTTCAAATGCTCACCCAAAGACATCTAAGTTGCAATTTAGTCGAGAGCAGATGAAGGAGATCTTTAGATATCATGATAACGATAAGGATGGTTTTCTCAATTTAAGGGAGCTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGGTATGTCATATGCGGATGCAAATAAAGATGGCCTTATCAGCGAGGCTGAACTTGACAAACTCATTGATTATGCTCAGAAATTTGTGAAGAAGAAATGA

Coding sequence (CDS)

ATGTTTATCTCCAAACTTCAAGCTTCCATGGCTAAGTACGAAGCAAGTGAGATGATCAAGAAGCATATTAGAAATGGTGATAATGTTCAACTACAATCTTTCAGTGGCATTGCTCGAGATTCAACTCAACTCAACAATCAAGGCAAGATACTAGAAATCCTAAAAGATAACAAGAATGTTGGGCTCAAGCTTAGTTCAAATGCTCACCCAAAGACATCTAAGTTGCAATTTAGTCGAGAGCAGATGAAGGAGATCTTTAGATATCATGATAACGATAAGGATGGTTTTCTCAATTTAAGGGAGCTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGGTATGTCATATGCGGATGCAAATAAAGATGGCCTTATCAGCGAGGCTGAACTTGACAAACTCATTGATTATGCTCAGAAATTTGTGAAGAAGAAATGA

Protein sequence

MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNNQGKILEILKDNKNVGLKLSSNAHPKTSKLQFSREQMKEIFRYHDNDKDGFLNLRELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK
Homology
[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.5e-41
Identity = 97/172 (56.40%), Postives = 121/172 (70.35%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNNQGKILEILKDNKNV 60
           M+ISKLQASMA YEAS +IK++ +NGD V++++F  + R STQ+ N+  +L+ILK +  V
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60

Query: 61  GLKLSSNAH--------------------PKTSKLQFSREQMKEIFRYHDNDKDGFLNLR 120
             KL S AH                     K+S +  SRE++KEIF+YHDN+ DGFLN  
Sbjct: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAFAFLGS+ PF KA YGM YADAN+DGLISEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.6e-38
Identity = 95/173 (54.91%), Postives = 119/173 (68.79%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLN-NQGKILEILKDNKN 60
           M I +LQ S AKYEAS+++KK+ +NG+ V L +F+  AR STQ+N N+    + LK N +
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87

Query: 61  VGLKLSSNAH--------------------PKTSKLQFSREQMKEIFRYHDNDKDGFLNL 120
           V  KLSSNA                      K S +QFSREQ+KEIF+Y+D+D+DGFLN+
Sbjct: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147

Query: 121 RELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           RE+TKAFA LGSI PF KA +GM YAD NKDGLI E ELDKLI+YA KF+KKK
Sbjct: 148 REVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 5.1e-37
Identity = 93/176 (52.84%), Postives = 118/176 (67.05%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNN-QGKILEIL--KDN 60
           M+IS LQ S AKYEAS+++KKH  N + V L++F+GI   ST++NN Q    EI+    N
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  KNVGLKLSSNAHP---------------------KTSKLQFSREQMKEIFRYHDNDKDGF 120
             V   L+SNA+                      K S +QFSREQ+KEIF+YHD+D DGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNLRELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           LNLRE+TKAFAFLG+I+P  KA +GM YADANKDGLIS+ EL+KL+DYA KF  K+
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.6e-35
Identity = 93/172 (54.07%), Postives = 118/172 (68.60%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNV----QLQSFSGIARDSTQLNNQGKILE--IL 60
           M +S LQA  AK EA+E+IKK+ RNGD V    +LQSF   AR STQLNN   +    + 
Sbjct: 1   MSVSHLQA-WAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVP 60

Query: 61  KDNKNVGLKLSSNA--------------HPKTSKLQFSREQMKEIFRYHDNDKDGFLNLR 120
           K N+N+  K+SSN                 K S +  SREQ+KEIF+YHD++KDGFL++R
Sbjct: 61  KTNENLAQKVSSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIR 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAF+ +GSI+PF KA Y M+YAD ++DGL+SEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFSSMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-21
Identity = 55/85 (64.71%), Postives = 64/85 (75.29%), Query Frame = 1 

Query: 68  AHPKTSKLQFSREQMKEIFRYHDNDKDGFLNLRELTKAFAFLGSIIPFEKASYGMSYADA 127
           A P  SK  FSRE+MKEIFR HD D DG+L++ EL KA  FLG  IPF KA YGM+Y D 
Sbjct: 2   ALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDE 61

Query: 128 NKDGLISEAELDKLIDYAQKFVKKK 153
           N DGLISE ELDKLIDYA++F K++
Sbjct: 62  NGDGLISEDELDKLIDYAERFQKRR 86

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 175.6 bits (444), Expect = 6.4e-41
Identity = 97/172 (56.40%), Postives = 121/172 (70.35%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNNQGKILEILKDNKNV 60
           M+ISKLQASMA YEAS +IK++ +NGD V++++F  + R STQ+ N+  +L+ILK +  V
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60

Query: 61  GLKLSSNAH--------------------PKTSKLQFSREQMKEIFRYHDNDKDGFLNLR 120
             KL S AH                     K+S +  SRE++KEIF+YHDN+ DGFLN  
Sbjct: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAFAFLGS+ PF KA YGM YADAN+DGLISEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|700194512|gb|KGN49689.1| (hypothetical protein Csa_5G067670 [Cucumis sativus])

HSP 1 Score: 167.2 bits (422), Expect = 2.3e-38
Identity = 95/173 (54.91%), Postives = 119/173 (68.79%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLN-NQGKILEILKDNKN 60
           M I +LQ S AKYEAS+++KK+ +NG+ V L +F+  AR STQ+N N+    + LK N +
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87

Query: 61  VGLKLSSNAH--------------------PKTSKLQFSREQMKEIFRYHDNDKDGFLNL 120
           V  KLSSNA                      K S +QFSREQ+KEIF+Y+D+D+DGFLN+
Sbjct: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147

Query: 121 RELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           RE+TKAFA LGSI PF KA +GM YAD NKDGLI E ELDKLI+YA KF+KKK
Sbjct: 148 REVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 162.2 bits (409), Expect = 7.4e-37
Identity = 93/176 (52.84%), Postives = 118/176 (67.05%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNN-QGKILEIL--KDN 60
           M+IS LQ S AKYEAS+++KKH  N + V L++F+GI   ST++NN Q    EI+    N
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  KNVGLKLSSNAHP---------------------KTSKLQFSREQMKEIFRYHDNDKDGF 120
             V   L+SNA+                      K S +QFSREQ+KEIF+YHD+D DGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNLRELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           LNLRE+TKAFAFLG+I+P  KA +GM YADANKDGLIS+ EL+KL+DYA KF  K+
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|449470190|ref|XP_004152801.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus])

HSP 1 Score: 157.1 bits (396), Expect = 2.4e-35
Identity = 93/172 (54.07%), Postives = 118/172 (68.60%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNV----QLQSFSGIARDSTQLNNQGKILE--IL 60
           M +S LQA  AK EA+E+IKK+ RNGD V    +LQSF   AR STQLNN   +    + 
Sbjct: 1   MSVSHLQA-WAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVP 60

Query: 61  KDNKNVGLKLSSNA--------------HPKTSKLQFSREQMKEIFRYHDNDKDGFLNLR 120
           K N+N+  K+SSN                 K S +  SREQ+KEIF+YHD++KDGFL++R
Sbjct: 61  KTNENLAQKVSSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIR 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAF+ +GSI+PF KA Y M+YAD ++DGL+SEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFSSMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|659089539|ref|XP_008445564.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo])

HSP 1 Score: 142.5 bits (358), Expect = 6.0e-31
Identity = 91/172 (52.91%), Postives = 113/172 (65.70%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNV----QLQSFSGIARDSTQLNNQGKILE--IL 60
           M +S LQA  AK EASE+IKK+ RNGD V    +LQSF   AR    L N   +    I 
Sbjct: 1   MSVSHLQA-WAKIEASELIKKYDRNGDGVLTKEELQSFLHDAR----LKNIRFVSSSTIP 60

Query: 61  KDNKNVGLKLSSNAHPKTSK--------------LQFSREQMKEIFRYHDNDKDGFLNLR 120
           K N+N+  K+SSN   K  K              +  SREQ+KEIF+YHD++KDGFL++R
Sbjct: 61  KTNENLDQKVSSNTRIKPKKQLLDKAQDSLRQSNVPLSREQIKEIFKYHDSNKDGFLSIR 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAF  LGSI+PF KA + M+YAD ++DGLISEAEL KLIDYA+K +KKK
Sbjct: 121 ELTKAFGSLGSIVPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

The following BLAST results are available for this feature:
BLAST of Cp4.1LG01g16960 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KMQ1_CUCSA4.5e-4156.40Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1[more]
A0A0A0KLF3_CUCSA1.6e-3854.91Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1[more]
A0A0A0KIY4_CUCSA5.1e-3752.84Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1[more]
A0A0A0LRC8_CUCSA1.6e-3554.07Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1[more]
A0A0A0LKX1_CUCSA1.4e-2164.71Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1[more]
BLAST of Cp4.1LG01g16960 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700194510|gb|KGN49687.1|6.4e-4156.40hypothetical protein Csa_5G067410 [Cucumis sativus][more]
gi|700194512|gb|KGN49689.1|2.3e-3854.91hypothetical protein Csa_5G067670 [Cucumis sativus][more]
gi|700194513|gb|KGN49690.1|7.4e-3752.84hypothetical protein Csa_5G068170 [Cucumis sativus][more]
gi|449470190|ref|XP_004152801.1|2.4e-3554.07PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus][more]
gi|659089539|ref|XP_008445564.1|6.0e-3152.91PREDICTED: probable calcium-binding protein CML11 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g16960.1Cp4.1LG01g16960.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 80..140
score: 4.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 77..112
score: 12.477coord: 113..148
score: 6
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 5..142
score: 1.6
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 73..140
score: 3.91
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 126..138
score: -coord: 90..102
scor
NoneNo IPR availableunknownCoilCoilcoord: 149..152
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 73..145
score: 1.6
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 73..145
score: 1.6

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG01g16960Wax gourdcpewgoB0559
Cp4.1LG01g16960Cucurbita maxima (Rimu)cmacpeB720
Cp4.1LG01g16960Cucurbita maxima (Rimu)cmacpeB732
Cp4.1LG01g16960Cucurbita moschata (Rifu)cmocpeB673
Cp4.1LG01g16960Cucurbita moschata (Rifu)cmocpeB680
Cp4.1LG01g16960Cucurbita moschata (Rifu)cmocpeB682
Cp4.1LG01g16960Cucurbita moschata (Rifu)cmocpeB686
Cp4.1LG01g16960Wild cucumber (PI 183967)cpecpiB449
Cp4.1LG01g16960Cucumber (Chinese Long) v2cpecuB449
Cp4.1LG01g16960Bottle gourd (USVL1VR-Ls)cpelsiB319
Cp4.1LG01g16960Watermelon (Charleston Gray)cpewcgB365
Cp4.1LG01g16960Watermelon (Charleston Gray)cpewcgB369
Cp4.1LG01g16960Watermelon (97103) v1cpewmB455
Cp4.1LG01g16960Melon (DHL92) v3.5.1cpemeB374
Cp4.1LG01g16960Cucumber (Gy14) v2cgybcpeB663
Cp4.1LG01g16960Melon (DHL92) v3.6.1cpemedB433
Cp4.1LG01g16960Cucumber (Chinese Long) v3cpecucB0557