Cp4.1LG01g16960 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG01g16960
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCalcium-binding EF-hand family protein, putative
LocationCp4.1LG01 : 11018886 .. 11019344 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTATCTCCAAACTTCAAGCTTCCATGGCTAAGTACGAAGCAAGTGAGATGATCAAGAAGCATATTAGAAATGGTGATAATGTTCAACTACAATCTTTCAGTGGCATTGCTCGAGATTCAACTCAACTCAACAATCAAGGCAAGATACTAGAAATCCTAAAAGATAACAAGAATGTTGGGCTCAAGCTTAGTTCAAATGCTCACCCAAAGACATCTAAGTTGCAATTTAGTCGAGAGCAGATGAAGGAGATCTTTAGATATCATGATAACGATAAGGATGGTTTTCTCAATTTAAGGGAGCTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGGTATGTCATATGCGGATGCAAATAAAGATGGCCTTATCAGCGAGGCTGAACTTGACAAACTCATTGATTATGCTCAGAAATTTGTGAAGAAGAAATGA

mRNA sequence

ATGTTTATCTCCAAACTTCAAGCTTCCATGGCTAAGTACGAAGCAAGTGAGATGATCAAGAAGCATATTAGAAATGGTGATAATGTTCAACTACAATCTTTCAGTGGCATTGCTCGAGATTCAACTCAACTCAACAATCAAGGCAAGATACTAGAAATCCTAAAAGATAACAAGAATGTTGGGCTCAAGCTTAGTTCAAATGCTCACCCAAAGACATCTAAGTTGCAATTTAGTCGAGAGCAGATGAAGGAGATCTTTAGATATCATGATAACGATAAGGATGGTTTTCTCAATTTAAGGGAGCTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGGTATGTCATATGCGGATGCAAATAAAGATGGCCTTATCAGCGAGGCTGAACTTGACAAACTCATTGATTATGCTCAGAAATTTGTGAAGAAGAAATGA

Coding sequence (CDS)

ATGTTTATCTCCAAACTTCAAGCTTCCATGGCTAAGTACGAAGCAAGTGAGATGATCAAGAAGCATATTAGAAATGGTGATAATGTTCAACTACAATCTTTCAGTGGCATTGCTCGAGATTCAACTCAACTCAACAATCAAGGCAAGATACTAGAAATCCTAAAAGATAACAAGAATGTTGGGCTCAAGCTTAGTTCAAATGCTCACCCAAAGACATCTAAGTTGCAATTTAGTCGAGAGCAGATGAAGGAGATCTTTAGATATCATGATAACGATAAGGATGGTTTTCTCAATTTAAGGGAGCTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGGTATGTCATATGCGGATGCAAATAAAGATGGCCTTATCAGCGAGGCTGAACTTGACAAACTCATTGATTATGCTCAGAAATTTGTGAAGAAGAAATGA

Protein sequence

MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNNQGKILEILKDNKNVGLKLSSNAHPKTSKLQFSREQMKEIFRYHDNDKDGFLNLRELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK
Homology
[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.5e-41
Identity = 97/172 (56.40%), Postives = 121/172 (70.35%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNNQGKILEILKDNKNV 60
           M+ISKLQASMA YEAS +IK++ +NGD V++++F  + R STQ+ N+  +L+ILK +  V
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60

Query: 61  GLKLSSNAH--------------------PKTSKLQFSREQMKEIFRYHDNDKDGFLNLR 120
             KL S AH                     K+S +  SRE++KEIF+YHDN+ DGFLN  
Sbjct: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAFAFLGS+ PF KA YGM YADAN+DGLISEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.6e-38
Identity = 95/173 (54.91%), Postives = 119/173 (68.79%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLN-NQGKILEILKDNKN 60
           M I +LQ S AKYEAS+++KK+ +NG+ V L +F+  AR STQ+N N+    + LK N +
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87

Query: 61  VGLKLSSNAH--------------------PKTSKLQFSREQMKEIFRYHDNDKDGFLNL 120
           V  KLSSNA                      K S +QFSREQ+KEIF+Y+D+D+DGFLN+
Sbjct: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147

Query: 121 RELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           RE+TKAFA LGSI PF KA +GM YAD NKDGLI E ELDKLI+YA KF+KKK
Sbjct: 148 REVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 5.1e-37
Identity = 93/176 (52.84%), Postives = 118/176 (67.05%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNN-QGKILEIL--KDN 60
           M+IS LQ S AKYEAS+++KKH  N + V L++F+GI   ST++NN Q    EI+    N
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  KNVGLKLSSNAHP---------------------KTSKLQFSREQMKEIFRYHDNDKDGF 120
             V   L+SNA+                      K S +QFSREQ+KEIF+YHD+D DGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNLRELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           LNLRE+TKAFAFLG+I+P  KA +GM YADANKDGLIS+ EL+KL+DYA KF  K+
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.6e-35
Identity = 93/172 (54.07%), Postives = 118/172 (68.60%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNV----QLQSFSGIARDSTQLNNQGKILE--IL 60
           M +S LQA  AK EA+E+IKK+ RNGD V    +LQSF   AR STQLNN   +    + 
Sbjct: 1   MSVSHLQA-WAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVP 60

Query: 61  KDNKNVGLKLSSNA--------------HPKTSKLQFSREQMKEIFRYHDNDKDGFLNLR 120
           K N+N+  K+SSN                 K S +  SREQ+KEIF+YHD++KDGFL++R
Sbjct: 61  KTNENLAQKVSSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIR 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAF+ +GSI+PF KA Y M+YAD ++DGL+SEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFSSMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-21
Identity = 55/85 (64.71%), Postives = 64/85 (75.29%), Query Frame = 1 

Query: 68  AHPKTSKLQFSREQMKEIFRYHDNDKDGFLNLRELTKAFAFLGSIIPFEKASYGMSYADA 127
           A P  SK  FSRE+MKEIFR HD D DG+L++ EL KA  FLG  IPF KA YGM+Y D 
Sbjct: 2   ALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDE 61

Query: 128 NKDGLISEAELDKLIDYAQKFVKKK 153
           N DGLISE ELDKLIDYA++F K++
Sbjct: 62  NGDGLISEDELDKLIDYAERFQKRR 86

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 175.6 bits (444), Expect = 6.4e-41
Identity = 97/172 (56.40%), Postives = 121/172 (70.35%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNNQGKILEILKDNKNV 60
           M+ISKLQASMA YEAS +IK++ +NGD V++++F  + R STQ+ N+  +L+ILK +  V
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60

Query: 61  GLKLSSNAH--------------------PKTSKLQFSREQMKEIFRYHDNDKDGFLNLR 120
             KL S AH                     K+S +  SRE++KEIF+YHDN+ DGFLN  
Sbjct: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAFAFLGS+ PF KA YGM YADAN+DGLISEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|700194512|gb|KGN49689.1| (hypothetical protein Csa_5G067670 [Cucumis sativus])

HSP 1 Score: 167.2 bits (422), Expect = 2.3e-38
Identity = 95/173 (54.91%), Postives = 119/173 (68.79%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLN-NQGKILEILKDNKN 60
           M I +LQ S AKYEAS+++KK+ +NG+ V L +F+  AR STQ+N N+    + LK N +
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87

Query: 61  VGLKLSSNAH--------------------PKTSKLQFSREQMKEIFRYHDNDKDGFLNL 120
           V  KLSSNA                      K S +QFSREQ+KEIF+Y+D+D+DGFLN+
Sbjct: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147

Query: 121 RELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           RE+TKAFA LGSI PF KA +GM YAD NKDGLI E ELDKLI+YA KF+KKK
Sbjct: 148 REVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 162.2 bits (409), Expect = 7.4e-37
Identity = 93/176 (52.84%), Postives = 118/176 (67.05%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNN-QGKILEIL--KDN 60
           M+IS LQ S AKYEAS+++KKH  N + V L++F+GI   ST++NN Q    EI+    N
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  KNVGLKLSSNAHP---------------------KTSKLQFSREQMKEIFRYHDNDKDGF 120
             V   L+SNA+                      K S +QFSREQ+KEIF+YHD+D DGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNLRELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           LNLRE+TKAFAFLG+I+P  KA +GM YADANKDGLIS+ EL+KL+DYA KF  K+
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|449470190|ref|XP_004152801.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus])

HSP 1 Score: 157.1 bits (396), Expect = 2.4e-35
Identity = 93/172 (54.07%), Postives = 118/172 (68.60%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNV----QLQSFSGIARDSTQLNNQGKILE--IL 60
           M +S LQA  AK EA+E+IKK+ RNGD V    +LQSF   AR STQLNN   +    + 
Sbjct: 1   MSVSHLQA-WAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVP 60

Query: 61  KDNKNVGLKLSSNA--------------HPKTSKLQFSREQMKEIFRYHDNDKDGFLNLR 120
           K N+N+  K+SSN                 K S +  SREQ+KEIF+YHD++KDGFL++R
Sbjct: 61  KTNENLAQKVSSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIR 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAF+ +GSI+PF KA Y M+YAD ++DGL+SEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFSSMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match: gi|659089539|ref|XP_008445564.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo])

HSP 1 Score: 142.5 bits (358), Expect = 6.0e-31
Identity = 91/172 (52.91%), Postives = 113/172 (65.70%), Query Frame = 1 

Query: 1   MFISKLQASMAKYEASEMIKKHIRNGDNV----QLQSFSGIARDSTQLNNQGKILE--IL 60
           M +S LQA  AK EASE+IKK+ RNGD V    +LQSF   AR    L N   +    I 
Sbjct: 1   MSVSHLQA-WAKIEASELIKKYDRNGDGVLTKEELQSFLHDAR----LKNIRFVSSSTIP 60

Query: 61  KDNKNVGLKLSSNAHPKTSK--------------LQFSREQMKEIFRYHDNDKDGFLNLR 120
           K N+N+  K+SSN   K  K              +  SREQ+KEIF+YHD++KDGFL++R
Sbjct: 61  KTNENLDQKVSSNTRIKPKKQLLDKAQDSLRQSNVPLSREQIKEIFKYHDSNKDGFLSIR 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 153
           ELTKAF  LGSI+PF KA + M+YAD ++DGLISEAEL KLIDYA+K +KKK
Sbjct: 121 ELTKAFGSLGSIVPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

The following BLAST results are available for this feature:
BLAST of Cp4.1LG01g16960 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of Cp4.1LG01g16960 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KMQ1_CUCSA4.5e-4156.40Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1[more]
A0A0A0KLF3_CUCSA1.6e-3854.91Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1[more]
A0A0A0KIY4_CUCSA5.1e-3752.84Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1[more]
A0A0A0LRC8_CUCSA1.6e-3554.07Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1[more]
A0A0A0LKX1_CUCSA1.4e-2164.71Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1[more]
BLAST of Cp4.1LG01g16960 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of Cp4.1LG01g16960 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700194510|gb|KGN49687.1|6.4e-4156.40hypothetical protein Csa_5G067410 [Cucumis sativus][more]
gi|700194512|gb|KGN49689.1|2.3e-3854.91hypothetical protein Csa_5G067670 [Cucumis sativus][more]
gi|700194513|gb|KGN49690.1|7.4e-3752.84hypothetical protein Csa_5G068170 [Cucumis sativus][more]
gi|449470190|ref|XP_004152801.1|2.4e-3554.07PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus][more]
gi|659089539|ref|XP_008445564.1|6.0e-3152.91PREDICTED: probable calcium-binding protein CML11 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g16960.1Cp4.1LG01g16960.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 80..140
score: 4.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 77..112
score: 12.477coord: 113..148
score: 6
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 5..142
score: 1.6
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 73..140
score: 3.91
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 126..138
score: -coord: 90..102
scor
NoneNo IPR availableunknownCoilCoilcoord: 149..152
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 73..145
score: 1.6
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 73..145
score: 1.6

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG01g16960Cucurbita maxima (Rimu)cmacpeB720
Cp4.1LG01g16960Cucurbita maxima (Rimu)cmacpeB732
Cp4.1LG01g16960Cucurbita moschata (Rifu)cmocpeB673
Cp4.1LG01g16960Cucurbita moschata (Rifu)cmocpeB680
Cp4.1LG01g16960Cucurbita moschata (Rifu)cmocpeB682
Cp4.1LG01g16960Cucurbita moschata (Rifu)cmocpeB686
Cp4.1LG01g16960Wild cucumber (PI 183967)cpecpiB449
Cp4.1LG01g16960Cucumber (Chinese Long) v2cpecuB449
Cp4.1LG01g16960Bottle gourd (USVL1VR-Ls)cpelsiB319
Cp4.1LG01g16960Watermelon (Charleston Gray)cpewcgB365
Cp4.1LG01g16960Watermelon (Charleston Gray)cpewcgB369
Cp4.1LG01g16960Watermelon (97103) v1cpewmB455
Cp4.1LG01g16960Melon (DHL92) v3.5.1cpemeB374
Cp4.1LG01g16960Cucumber (Gy14) v2cgybcpeB663
Cp4.1LG01g16960Melon (DHL92) v3.6.1cpemedB433
Cp4.1LG01g16960Cucumber (Chinese Long) v3cpecucB0557
Cp4.1LG01g16960Wax gourdcpewgoB0559