Cp4.1LG01g14270 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG01g14270
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	3-oxo-5-alpha-steroid 4-dehydrogenase family protein
Location	Cp4.1LG01 : 7816590 .. 7820342 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGATGTGAATTTACGGAAATGCCCATCTGCAATTTCAATGTAACGTGCGGTATACAGCACCACTGGTTCTCGGCTCGACCCGAAGGTTCAATCCCCTAAACTGAAAATGGTTGCGATCTATAAATCCTCATTTCAAACGCCTCCGACGGCGTTTCAAACTTCTACAGAGAGTTTCTGGCGGTTTTTCTCATCTGGTTCTCGGCGGCGATGGGTATTTTGTCGGCGTTTCTTTTCCCTCCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCTGGGATTTCCGAGATTAAAGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTGCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTCGAGGTACTCTCAATTTCTCTACGCCTCTGATTGAGTTCTGAAATTTCAATCCGATTTTAAAATTAATTCTTCGATTCAGTTCGATTGTGGCTTTATTGAGAATCTGTTAATCTTATTCTTCGAAAATTCGTGGCGAAAAGAAAATGGCGGAGAGATCTGGATCTCTTCTCCCTTCTTTGCATCTTATGGTTCCTCTGGAGATTTCGGAACTCGTCTTTTCGTTTACCGGATTAGGAAGACTTTGAACCTATCTTGATTTCTTTTTTTTTTTTATTTTTATAATTTTTTGTCGTATGAACTTGAATAATTAACCATCCACTTTACAAATATCCGACTTCATTAGTTTTCCTCAACTAATTTAATTGTCATTAAGAACTCGTGAAGTCAACTTTGTGTATAATAATAATTTTGGAATTATTATTATATGAAGTCATGTACAAGAAAGAACAAACTGCACGTTTCTGCTCAACAGTTTTCTAAGAAATTTGTTTCAATAATCACTAGATGATTTGCACTTATTGTACCTTTCATTCATGCGACAATTATTTTTGAATCAATTCGAGTATTCTTCTCAAATTATAGAAGCTATTCCTGTATTATTTCTCCAACCAATTGCATCTGAGGGGAACTAGAAATGATAACAAAAGTTTTGGAAGATCATATGAATCATTTAGAGAACAATGAAAAGTAATATAAACAATTTCAAAAACAACAAAACACCTAATCTTATATTAAATATATCATTGAATTCAAAAGCTTAAACGGATAGACAATATGAATTACTTACTATCATGAAATTCTTTGCAGGTTCTATTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTCGTCCACGGTGATCATGATATACACACAGCACCTATCACAAGGACTTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCATTTTCTCTTGTCCAAAACCAGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCCTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGGTACGTCTCCAAATTTGAAAACTTCCCCAGCCATGTCAAAGCCCTGCTCCCTTTTGTGTTTTAGAACCCTTCCTTTCTTCATTTCATAAAAGCTACTTATGTTTATGCTTTACACAATTAAGTTAGGCTCATACTACTATTGTATTAGTTCCCTTAAGTTCTGATTCTGTTTATCACTACAATAGTAGAAGGTAAAGCAGTTGGATTTTGTTCTTTACCTGATCTTAATAATGACCATTTATACTTGTGTGTTTTTATTTATTAAAATTAAAATACTTTCTATGTTATGAAATTGAATTGAATTAAATTAAAAATGTTATTGAAACCTTAATCTGGAAAATTAGTCTAAAACTCCTAGGTTATATATTTGTTGTTTTAGAAAAAAACGCTCGTTCATGCAAGTGTTTCTATGGACTTTGTCGTCGACCAAATTGGGTTGTTCGTATTTGATAATAATATTGATTATCATCCTCCGTTGATAGCATTCGGCCCTAGTATAGAAGAGGAGTATCTAAAAAAATTCGATGATCCGAAAAACTCGATCAACCCATCCAAACCTATACAAATGTGTAATAAAATAAAATAAAAATTTATAGTTAGGTTCATTATTTTAAAAAAAAAAAAAAAATTATACAGAGTCTAATCCAACCCATTAACACATACATTCTATTTTTTTATTTAAAAAAAAAAAACTAATTTCTTAGAAAATAAATATATGTAAATTTTGTTTACTCTTTTTTTATAAAAATTAAAAATTAAGAGGATAAAAATTGTTATTTTCTAGATTTATAAATAAGGTTGCAAACTATCAAAAATAAAAATATTTTTAGAATATTCAGCTAAAATAAATCATAAATAATACTAGAAAAAAGAAAAAAATAATAAAAAAAACAAGAAATGTATTATATATATATATATACACTTAATTATGGAATGGCCACACACTAAGAAAGAAAAGGACATGAAGGAGATTTTTTTTTTTATAAATTGCAGAGCGATGAGGCAATCGTCGTTCACCATTAAATTTCCATCCTTCTCTGCAACTCACGATTTCAAGAGCAAAGACGACGGCCACCACCACCGCCGCCGCCGCCGCCGCCTCCATCTTCTGGTCCTTCTCCCATGCCGACATTCCTCTTTTCCCCATTTTCTTCTACCTTCCTCCTCGCTCTTTCTTCCCTCACCATGCTTTCCATTTTGGTCCTCGCTACGTCGGAATTTCTCGGCCGCCACTTGCAGTATTCCAAATTCTGGAGCCCTAAAACCTCCTCCAACAAAATCCACCTCCCTTCCCGATCCGCTATGCTCCTCTGTTACGTCCCTGCTTTCGCCGTCGCCTCCATCTCCCTCTTCCTCCTCCTCCTCCCGCGCTACAATAACTCCTCCACGAGGATTTTCCTAATCAACTCCCTTCTCCTCATCCATTTCTTCAAACGAATCTTCGAGGTACGTCTACTTAACTAATTAGATCCTCTCGATTCTGTCTGTTCTGGAAGTTAATTAGCGAAATCGAAACTCGATTATTTCAGGTTTTGTTCGTTCATAAATACAGCAACAAAATGGGCCTGAGCGACGCGATTAGAGTCTCGATTACCTATGTTCTCCTTACCGCTAGCACAATCTCCTCTCAGATGGTTTCGCATAATCTGGAGGAGCCGCAGATCGATCTGAGGGCGGCTGGATTGGCGATGTACTGGATTGGAATCACTGGAAATCTGTATCACCACGTTCTACTAGCGAAATTGAGGAAAGAAGGCGATAGAGAGTACAGGATTCCAAGGGGAGGGATGTTTGAAGTGATCGTGTGCCCTCACTACCTGTTTGAGATGGTAGAGTTCTTGGGAATCGCTTTCGTTTCGCAGACGCTGTTCCCAATGGTGTTCGCCTTCGGATCCGCCCTGTTCTTGATTGGCCGAAGCTACGCCACCAGGAATTGGTACGTTTCTAAATTTGAAGATTTTCCTCGCCATGTTAAAGCTATTCTGCCTTTCGTTTTTTGA

mRNA sequence

ATGAAAGATTTTCTGGCGGTTTTTCTCATCTGGTTCTCGGCGGCGATGGGTATTTTGTCGGCGTTTCTTTTCCCTCCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCTGGGATTTCCGAGATTAAAGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTGCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTCGAGGTTCTATTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTCGTCCACGGTGATCATGATATACACACAGCACCTATCACAAGGACTTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCATTTTCTCTTGTCCAAAACCAGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCCTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGGTACGTCTCCAAATTTGAAAACTTCCCCAGCCATGTCAAAGCCCTGCTCCCTTTTAGCGATGAGGCAATCGTCCAAAGACGACGGCCACCACCACCGCCGCCGCCGCCGCCGCCTCCATCTTCTGGTCCTTCTCCCATGCCGACATTCCTCTTTTCCCCATTTTCTTCTACCTTCCTCCTCGCTCTTTCTTCCCTCACCATGCTTTCCATTTTGGTCCTCGCTACGTCGGAATTTCTCGGCCGCCACTTGCAGTATTCCAAATTCTGGAGCCCTAAAACCTCCTCCAACAAAATCCACCTCCCTTCCCGATCCGCTATGCTCCTCTGTTACGTCCCTGCTTTCGCCGTCGCCTCCATCTCCCTCTTCCTCCTCCTCCTCCCGCGCTACAATAACTCCTCCACGAGGATTTTCCTAATCAACTCCCTTCTCCTCATCCATTTCTTCAAACGAATCTTCGAGGTTTTGTTCGTTCATAAATACAGCAACAAAATGGGCCTGAGCGACGCGATTAGAGTCTCGATTACCTATGTTCTCCTTACCGCTAGCACAATCTCCTCTCAGATGGTTTCGCATAATCTGGAGGAGCCGCAGATCGATCTGAGGGCGGCTGGATTGGCGATGTACTGGATTGGAATCACTGGAAATCTGTATCACCACGTTCTACTAGCGAAATTGAGGAAAGAAGGCGATAGAGAGTACAGGATTCCAAGGGGAGGGATGTTTGAAGTGATCGTGTGCCCTCACTACCTGTTTGAGATGGTAGAGTTCTTGGGAATCGCTTTCGTTTCGCAGACGCTGTTCCCAATGGTGTTCGCCTTCGGATCCGCCCTGTTCTTGATTGGCCGAAGCTACGCCACCAGGAATTGGTACGTTTCTAAATTTGAAGATTTTCCTCGCCATGTTAAAGCTATTCTGCCTTTCGTTTTTTGA

Coding sequence (CDS)

ATGAAAGATTTTCTGGCGGTTTTTCTCATCTGGTTCTCGGCGGCGATGGGTATTTTGTCGGCGTTTCTTTTCCCTCCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCTGGGATTTCCGAGATTAAAGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTGCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTCGAGGTTCTATTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTCGTCCACGGTGATCATGATATACACACAGCACCTATCACAAGGACTTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCATTTTCTCTTGTCCAAAACCAGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCCTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGGTACGTCTCCAAATTTGAAAACTTCCCCAGCCATGTCAAAGCCCTGCTCCCTTTTAGCGATGAGGCAATCGTCCAAAGACGACGGCCACCACCACCGCCGCCGCCGCCGCCGCCTCCATCTTCTGGTCCTTCTCCCATGCCGACATTCCTCTTTTCCCCATTTTCTTCTACCTTCCTCCTCGCTCTTTCTTCCCTCACCATGCTTTCCATTTTGGTCCTCGCTACGTCGGAATTTCTCGGCCGCCACTTGCAGTATTCCAAATTCTGGAGCCCTAAAACCTCCTCCAACAAAATCCACCTCCCTTCCCGATCCGCTATGCTCCTCTGTTACGTCCCTGCTTTCGCCGTCGCCTCCATCTCCCTCTTCCTCCTCCTCCTCCCGCGCTACAATAACTCCTCCACGAGGATTTTCCTAATCAACTCCCTTCTCCTCATCCATTTCTTCAAACGAATCTTCGAGGTTTTGTTCGTTCATAAATACAGCAACAAAATGGGCCTGAGCGACGCGATTAGAGTCTCGATTACCTATGTTCTCCTTACCGCTAGCACAATCTCCTCTCAGATGGTTTCGCATAATCTGGAGGAGCCGCAGATCGATCTGAGGGCGGCTGGATTGGCGATGTACTGGATTGGAATCACTGGAAATCTGTATCACCACGTTCTACTAGCGAAATTGAGGAAAGAAGGCGATAGAGAGTACAGGATTCCAAGGGGAGGGATGTTTGAAGTGATCGTGTGCCCTCACTACCTGTTTGAGATGGTAGAGTTCTTGGGAATCGCTTTCGTTTCGCAGACGCTGTTCCCAATGGTGTTCGCCTTCGGATCCGCCCTGTTCTTGATTGGCCGAAGCTACGCCACCAGGAATTGGTACGTTTCTAAATTTGAAGATTTTCCTCGCCATGTTAAAGCTATTCTGCCTTTCGTTTTTTGA

Protein sequence

MKDFLAVFLIWFSAAMGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEPKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPFSDEAIVQRRRPPPPPPPPPPPSSGPSPMPTFLFSPFSSTFLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPKTSSNKIHLPSRSAMLLCYVPAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMGLSDAIRVSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYATRNWYVSKFEDFPRHVKAILPFVF

Homology

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BLAST of Cp4.1LG01g14270 vs. Swiss-Prot
Match: S5A1_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 5.8e-18
Identity = 44/105 (41.90%), Postives = 64/105 (60.95%), Query Frame = 1


Query: 175 IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 234
           IG  L+L G++ N +   +L   RKPG+TGYKIP+GGLF  V   +Y  EI+E+ G+A  
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 235 SQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K L+PF
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPF 261

[Close]

BLAST of Cp4.1LG01g14270 vs. Swiss-Prot
Match: S5A1_MOUSE (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 93.2 bits (230), Expect = 9.8e-18
Identity = 72/247 (29.15%), Postives = 114/247 (46.15%), Query Frame = 1


Query: 43  MGMAGISEIKGKHLQYSKFWNANSAPKAPS----EEPKLSSKYGMLLAYTPAFLAGAASF 102
           +   G+  +   + +YS  W+    P  P+    E P ++  + +     PA    AA  
Sbjct: 22  VAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMA--WPLYECIRPA----AARL 81

Query: 103 WFFPSDNERILVLKAALTLHFFKR--IFEVLFIHKYSSKMVFDTAIKISF----SYCSST 162
              P+      VL A   +H+ +R  +F VL I      ++F   +   F     Y  S 
Sbjct: 82  GNLPNR-----VLLAMFLIHYVQRTLVFPVL-IRGGKPTLLFTFVLAFLFCTLNGYLQSR 141

Query: 163 VIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGL 222
            +  +  +    +  P       G AL+L+G++ N +   +L   RKPGETGYKIP+GGL
Sbjct: 142 YLSQFAVYAEDWVTHPCF---LTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGL 201

Query: 223 FSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSH 280
           F  V   +Y  E++E+ GFA  S  L  + F+  T   L  R+    +WY+ KFE++P  
Sbjct: 202 FEYVSSANYFGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKT 253

[Close]

BLAST of Cp4.1LG01g14270 vs. Swiss-Prot
Match: S5A1_RAT (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 1.3e-17
Identity = 45/104 (43.27%), Postives = 63/104 (60.58%), Query Frame = 1


Query: 176 GIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFIS 235
           G AL+L+G++ N +   +L   RKPGETGYKIP+GGLF  V   +Y  E++E+ GFA  S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213

Query: 236 QELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
             L  + F+  T   L  R+    +WY  KFE++P   K L+PF
Sbjct: 214 WSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPF 257

[Close]

BLAST of Cp4.1LG01g14270 vs. Swiss-Prot
Match: S5A1_HUMAN (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.3e-17
Identity = 55/172 (31.98%), Postives = 88/172 (51.16%), Query Frame = 1


Query: 110 VLKAALTLHFFKRIFEVLFIHKYSSKM-VFDTAIKISFSYCSSTVIMIYTQHLSQGLPEP 169
           +L A   +H+  R     F+ +    M +    + I F  C+  +   Y  H +    + 
Sbjct: 86  ILLAMFLVHYGHRCLIYPFLMRGGKPMPLLACTMAIMFCTCNGYLQSRYLSHCAVYADDW 145

Query: 170 PIDLKN-IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIE 229
             D +  IG  L+L G++ N +   +L   RKPG+TGYKIP+GGLF  V   +Y  EI+E
Sbjct: 146 VTDPRFLIGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIME 205

Query: 230 FFGFAFISQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
           + G+A  S  +    F+  T  +L+GR+    +WY+ KFE +P   K ++PF
Sbjct: 206 WCGYALASWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPF 257

[Close]

BLAST of Cp4.1LG01g14270 vs. Swiss-Prot
Match: S5A1_BOVIN (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus GN=SRD5A1 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 8.3e-17
Identity = 56/176 (31.82%), Postives = 88/176 (50.00%), Query Frame = 1


Query: 110 VLKAALTLHFFKRIFEVLFIHKYSSKMVFDTAIKISFSYCS------STVIMIYTQHLSQ 169
           +L A   +H+ +R     F+ +    M    A  ++F +C+      S  +  Y  +   
Sbjct: 84  ILLAMFLVHYAQRSLVFPFLIRGGKPMPL-YAFLLAFIFCTYNGYLQSRYLSQYAVYADD 143

Query: 170 GLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLF 229
            L +P       G AL+LIG++ N +   +L   RKPGETGYKIP+GGLF  +   +Y  
Sbjct: 144 WLSDPRF---LTGSALWLIGMLINIHSDHVLRNLRKPGETGYKIPRGGLFEYISAANYFG 203

Query: 230 EIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
           E++E+ G+A  S  +    F+  T   L  R+    KWY  KFE++P   K ++PF
Sbjct: 204 EVVEWCGYALASWSIQGWAFAVFTFCVLFTRAQQHHKWYHEKFEDYPKFRKIMIPF 255

[Close]

BLAST of Cp4.1LG01g14270 vs. TrEMBL
Match: A0A103Y9N2_CYNCS (3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal OS=Cynara cardunculus var. scolymus GN=Ccrd_016565 PE=4 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 3.3e-137
Identity = 275/559 (49.19%), Postives = 364/559 (65.12%), Query Frame = 1


Query: 18  ILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEPKL 77
           +L  FLFP P S FV  M VV+ +A+  A   EIKGKHL+YSKF NA    K   ++ KL
Sbjct: 6   VLLNFLFPSPSSWFVTAMLVVNLSALTDAAYMEIKGKHLRYSKFLNAGGVAK--KQDMKL 65

Query: 78  SSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKMV 137
           SS+ GMLL YTPAFL G ASF+  P  + R ++L +ALTLHFFKR+ EVLF+HKYS  M 
Sbjct: 66  SSRNGMLLFYTPAFLVGVASFFVVPYQDLRFVLLASALTLHFFKRVLEVLFLHKYSGSMA 125

Query: 138 FDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKT 197
            ++ I I+ SY SST  MIY Q LS+ +PEP +DLK +GI LF IG IGNFYHH LL+  
Sbjct: 126 LESPIIITMSYTSSTATMIYAQFLSRDVPEPTVDLKYVGIGLFFIGTIGNFYHHCLLANL 185

Query: 198 RKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYA 257
           R  G+  YKIP+GGLF LVICPHYLFEI+ + G + ISQ  Y   F+ GT  YL GRSY 
Sbjct: 186 RNKGDKEYKIPQGGLFGLVICPHYLFEILAYAGISSISQTPYAFAFTLGTIFYLIGRSYG 245

Query: 258 TRKWYVSKF-ENFPSHVKALLPFSDEAIVQRRRPPPPPPPPPPPSSGPSPMPTFLFSPFS 317
           TR WY+SKF + FP  VKAL+P+ + A   RR   P         + PS     LF P S
Sbjct: 246 TRGWYLSKFGDKFPKDVKALIPYCEFANNLRR--TPNTLRLIIKMAIPSIFLNLLFPPPS 305

Query: 318 STFLLALSSLTMLSILVLATSEFLGRHLQYSKFWS----PKTSSNKIHLPSRSAMLLCYV 377
           S F+  ++ +   S+      E  G+H+QYSKF +     K    ++ L S++ MLL Y 
Sbjct: 306 SLFVTTMTVVGFSSLTNAGYKEIKGQHMQYSKFLNVGVVEKEKEKEMKLSSKNGMLLFYA 365

Query: 378 PAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMGLSDAIR 437
           P+F V   S F      + +   R  L+ S L +HFFKR+ EVLFVHKYS  M L  AI 
Sbjct: 366 PSFLVGLTSFF-----AFPHEDLRFGLLVSALTVHFFKRVVEVLFVHKYSGSMALESAII 425

Query: 438 VSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDR 497
           V ++Y   TA+ I +Q++S ++ EP +DL+  G+ ++ IG  GN YHH LLA LRK+GD+
Sbjct: 426 VPLSYAFSTATMIYTQILSRDVPEPLLDLKYVGVGLFIIGEIGNFYHHWLLANLRKKGDK 485

Query: 498 EYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYATRNWYV 557
           EY+IP+GG+F++++CPHYLFE++ ++GIA +SQTL+   F  G+  +L GRSY TR WY+
Sbjct: 486 EYKIPKGGLFDLVICPHYLFEILAYVGIASISQTLYAFSFTLGTIFYLTGRSYTTREWYL 545

Query: 558 SKFED-FPRHVKAILPFVF 571
           SKF D FP++VKA +P++F
Sbjct: 546 SKFGDKFPKNVKAFIPYIF 555

[Close]

BLAST of Cp4.1LG01g14270 vs. TrEMBL
Match: A0A0A0KTC7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612880 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.0e-114
Identity = 211/275 (76.73%), Postives = 230/275 (83.64%), Query Frame = 1


Query: 5   LAVFLIWFSAAMGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNA 64
           L +F IW SAAM +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEI GKHLQYSKFWNA
Sbjct: 10  LKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNA 69

Query: 65  NSAPKAPSEEPKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIF 124
           N A K  +EE K+SSKYGMLLAYTPAFLAGAASFW FPS +ER                 
Sbjct: 70  NGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDER----------------- 129

Query: 125 EVLFIHKYSSKMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGI 184
            VLFIHKYSSKMV  TAI+IS SY  ST IMIYTQHLSQGLPEPPIDLKNIG+  FLIGI
Sbjct: 130 -VLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGI 189

Query: 185 IGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFS 244
           IGNFYHH+LLS+TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS
Sbjct: 190 IGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFS 249

Query: 245 TGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
            GTA+YLAGRSYATRKWY+SKFE FP++VKALLPF
Sbjct: 250 IGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPF 266

[Close]

BLAST of Cp4.1LG01g14270 vs. TrEMBL
Match: A0A166DSU9_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_006469 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.1e-112
Identity = 232/558 (41.58%), Postives = 330/558 (59.14%), Query Frame = 1


Query: 19  LSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEPKLS 78
           L  F++PPP S F+N MTV++   +   G+ E+KGK +QYSKF+N +      S+  +LS
Sbjct: 6   LLRFIYPPPASWFINAMTVITGAILVNGGLMEVKGKSMQYSKFYNTSKKNAEASKITELS 65

Query: 79  SKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKMVF 138
            + GML+ Y PAF+A      F   +  R  ++ +ALT+HFFKR+FEV FIH YS  M  
Sbjct: 66  GRKGMLVVYAPAFVASVIFLAFMRDEGLRFTLVTSALTVHFFKRLFEVSFIHNYSGSMPI 125

Query: 139 DTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTR 198
           D  I  S SY   TV + Y+QHL   LPEP IDLK  G A FL+GI GNFYHH+LLSK R
Sbjct: 126 DIVINFSASYFLYTVTVTYSQHLVAELPEPVIDLKYAGTASFLVGITGNFYHHYLLSKLR 185

Query: 199 KPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYAT 258
           K GE  YKIP+G      +C           G ++ +++                     
Sbjct: 186 KEGEKQYKIPQG-----AVC---------LMGRSYATRKW-------------------- 245

Query: 259 RKWYVSKFENFPSHVKALLPFSDEAIVQRRRPPPPPPPPPPPSSGPSPMPTFLFSPFSST 318
              Y+SKFE+FP  VKAL+P    A+V                   S +  F++ P +S 
Sbjct: 246 ---YLSKFEDFPKDVKALIP----AMVL------------------STLLRFIYPPPASW 305

Query: 319 FLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPK------TSSNKIHLPSRSAMLLCYV 378
           F+ A++ +   S++     E  G+ +QYSKF++        + +  I L  R+ ML+ Y 
Sbjct: 306 FITAMTVINGASLVNAGLMEIKGKSMQYSKFFNTNKKNVEASGAKIIELSGRNGMLVAYG 365

Query: 379 PAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMGLSDAIR 438
           PAF  +  SL  +      N   R  L++S L +HFFKR+FEVLF+HKYS  M +  AI 
Sbjct: 366 PAFVASVTSLAFM-----PNEGLRFTLVSSALAVHFFKRLFEVLFIHKYSGSMPIDSAIV 425

Query: 439 VSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDR 498
           +S +Y L +  TI SQ +     EP +DL+ AG+ ++ +GI GN YHH LL+KLRKEGD+
Sbjct: 426 ISTSYFLSSLCTIYSQHLCGQFPEPVVDLKYAGITIFLVGIVGNFYHHYLLSKLRKEGDK 485

Query: 499 EYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYATRNWYV 558
           EY+IP+GG+F++++CPHYLFE++ F+GI+ +SQT++ + F  G+  +L GRSYATR WY+
Sbjct: 486 EYKIPQGGLFDLVICPHYLFEILGFIGISCMSQTVYALSFTAGTIGYLTGRSYATRRWYL 499

Query: 559 SKFEDFPRHVKAILPFVF 571
           SKFEDFP+ VKA++P++F
Sbjct: 546 SKFEDFPKDVKALIPYIF 499

[Close]

BLAST of Cp4.1LG01g14270 vs. TrEMBL
Match: A0A0A0KWK8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612900 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.8e-103
Identity = 192/265 (72.45%), Postives = 220/265 (83.02%), Query Frame = 1


Query: 16  MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 75
           M  LS F+FPPP S FVNVMTVVSS  + + G+SE +GKHL+YSKFW++N+A     +  
Sbjct: 1   MDTLSEFVFPPPASAFVNVMTVVSSAIVAVVGLSEARGKHLKYSKFWSSNNA-NTDRKSF 60

Query: 76  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 135
            LS+K GML+AY PAFLAG  S W FPS++ RIL+LK+AL LHFFKR  EVLF+HKYSSK
Sbjct: 61  NLSAKLGMLVAYIPAFLAGVVSLWLFPSEDRRILLLKSALVLHFFKRNLEVLFLHKYSSK 120

Query: 136 MVFDTAIKISFSYCSSTVIMIYTQHLSQGL-PEPPIDLKNIGIALFLIGIIGNFYHHFLL 195
           M  D+ I ISFSY SST IMIYTQHLSQGL  EPPIDLK+IG+ LFLIGIIGNFYHH+LL
Sbjct: 121 MAVDSVITISFSYFSSTAIMIYTQHLSQGLLREPPIDLKSIGVGLFLIGIIGNFYHHYLL 180

Query: 196 SKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGR 255
           S+TRK GET YKIPKGGLFSL+ICPHYLFEIIEFFGFAFISQ +Y L F+  TA+YL+GR
Sbjct: 181 SQTRKQGETSYKIPKGGLFSLIICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLSGR 240

Query: 256 SYATRKWYVSKFENFPSHVKALLPF 280
           SYATRKWYVSKFE+FP HVKALLPF
Sbjct: 241 SYATRKWYVSKFEDFPIHVKALLPF 264

[Close]

BLAST of Cp4.1LG01g14270 vs. TrEMBL
Match: A0A0A0KRA7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612890 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 5.0e-101
Identity = 187/270 (69.26%), Postives = 218/270 (80.74%), Query Frame = 1


Query: 12  FSAAMGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWN-ANSAPKA 71
           F+AAM  LSAF FP PPS  VNVMTVVSS  + + G SE +G HLQYSKFWN +NSA K+
Sbjct: 3   FTAAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKS 62

Query: 72  P-SEEPKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFI 131
             SE+ ++SS+ GMLL YTPA LAGAASFWF P+D+ R+L+LK+ALT HFFKR FEVLF+
Sbjct: 63  SNSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFV 122

Query: 132 HKYSSKMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFY 191
           HKYS KMV D+A+ IS SY SST +MIY Q  S+ LPEP IDLKNIGIA+F+IGIIGN Y
Sbjct: 123 HKYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMY 182

Query: 192 HHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAI 251
           HH LLS+TRK GETGYKIPKGGLF ++ICPHY+FEI  +FGFA ISQ  Y   F+  TAI
Sbjct: 183 HHILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAI 242

Query: 252 YLAGRSYATRKWYVSKFENFPSHVKALLPF 280
           YL GRSYATRKWYVSKFE+FP H+KAL+PF
Sbjct: 243 YLGGRSYATRKWYVSKFEDFPQHIKALIPF 272

[Close]

BLAST of Cp4.1LG01g14270 vs. TAIR10
Match: AT5G16010.1 (AT5G16010.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 311.6 bits (797), Expect = 9.9e-85
Identity = 151/266 (56.77%), Postives = 201/266 (75.56%), Query Frame = 1


Query: 16  MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEE- 75
           M ++++F++PPPPS  +N M+VV   A+   G SEI+G HL+YSKF  ++S+P+   E  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 76  PKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSS 135
             +SS+ GMLL YTPAFLA A+SF+  PSD+ R L+LK+AL LHFFKR+FEVLFIHKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 136 KMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLL 195
            M  D+A+ IS SY SST +M+Y+Q+L+ GL EP  D+K  G+ +F++GI+GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 196 SKTRK-PGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAG 255
           +K RK  G+  YKIPKGGLF ++ICPHYLFEI+ F+ F  ISQ +Y   F+ GT +YL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 256 RSYATRKWYVSKFENFPSHVKALLPF 280
           RSYATR WY+SKF++FP H+KAL+PF
Sbjct: 241 RSYATRTWYLSKFDDFPKHIKALIPF 266

[Close]

BLAST of Cp4.1LG01g14270 vs. TAIR10
Match: AT2G38050.1 (AT2G38050.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 74.7 bits (182), Expect = 2.0e-13
Identity = 40/106 (37.74%), Postives = 60/106 (56.60%), Query Frame = 1


Query: 175 IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 234
           IG+ +F+ G+  N      L + +K    GY IP+GG F LV CP+Y  E IE+ G+A +
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 235 SQELYPLCFSTGTAIYLAGRSYATRKWYVSKF-ENFPSHVKALLPF 280
           +     + F   T   L  R+ A+ KWY++KF E +P   KA++PF
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPF 260

[Close]

BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: gi|976920344|gb|KVI05075.1| (3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal [Cynara cardunculus var. scolymus])

HSP 1 Score: 496.9 bits (1278), Expect = 4.7e-137
Identity = 275/559 (49.19%), Postives = 364/559 (65.12%), Query Frame = 1


Query: 18  ILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEPKL 77
           +L  FLFP P S FV  M VV+ +A+  A   EIKGKHL+YSKF NA    K   ++ KL
Sbjct: 6   VLLNFLFPSPSSWFVTAMLVVNLSALTDAAYMEIKGKHLRYSKFLNAGGVAK--KQDMKL 65

Query: 78  SSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKMV 137
           SS+ GMLL YTPAFL G ASF+  P  + R ++L +ALTLHFFKR+ EVLF+HKYS  M 
Sbjct: 66  SSRNGMLLFYTPAFLVGVASFFVVPYQDLRFVLLASALTLHFFKRVLEVLFLHKYSGSMA 125

Query: 138 FDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKT 197
            ++ I I+ SY SST  MIY Q LS+ +PEP +DLK +GI LF IG IGNFYHH LL+  
Sbjct: 126 LESPIIITMSYTSSTATMIYAQFLSRDVPEPTVDLKYVGIGLFFIGTIGNFYHHCLLANL 185

Query: 198 RKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYA 257
           R  G+  YKIP+GGLF LVICPHYLFEI+ + G + ISQ  Y   F+ GT  YL GRSY 
Sbjct: 186 RNKGDKEYKIPQGGLFGLVICPHYLFEILAYAGISSISQTPYAFAFTLGTIFYLIGRSYG 245

Query: 258 TRKWYVSKF-ENFPSHVKALLPFSDEAIVQRRRPPPPPPPPPPPSSGPSPMPTFLFSPFS 317
           TR WY+SKF + FP  VKAL+P+ + A   RR   P         + PS     LF P S
Sbjct: 246 TRGWYLSKFGDKFPKDVKALIPYCEFANNLRR--TPNTLRLIIKMAIPSIFLNLLFPPPS 305

Query: 318 STFLLALSSLTMLSILVLATSEFLGRHLQYSKFWS----PKTSSNKIHLPSRSAMLLCYV 377
           S F+  ++ +   S+      E  G+H+QYSKF +     K    ++ L S++ MLL Y 
Sbjct: 306 SLFVTTMTVVGFSSLTNAGYKEIKGQHMQYSKFLNVGVVEKEKEKEMKLSSKNGMLLFYA 365

Query: 378 PAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMGLSDAIR 437
           P+F V   S F      + +   R  L+ S L +HFFKR+ EVLFVHKYS  M L  AI 
Sbjct: 366 PSFLVGLTSFF-----AFPHEDLRFGLLVSALTVHFFKRVVEVLFVHKYSGSMALESAII 425

Query: 438 VSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDR 497
           V ++Y   TA+ I +Q++S ++ EP +DL+  G+ ++ IG  GN YHH LLA LRK+GD+
Sbjct: 426 VPLSYAFSTATMIYTQILSRDVPEPLLDLKYVGVGLFIIGEIGNFYHHWLLANLRKKGDK 485

Query: 498 EYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYATRNWYV 557
           EY+IP+GG+F++++CPHYLFE++ ++GIA +SQTL+   F  G+  +L GRSY TR WY+
Sbjct: 486 EYKIPKGGLFDLVICPHYLFEILAYVGIASISQTLYAFSFTLGTIFYLTGRSYTTREWYL 545

Query: 558 SKFED-FPRHVKAILPFVF 571
           SKF D FP++VKA +P++F
Sbjct: 546 SKFGDKFPKNVKAFIPYIF 555

[Close]

BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: gi|659091769|ref|XP_008446722.1| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo])

HSP 1 Score: 465.3 bits (1196), Expect = 1.5e-127
Identity = 229/272 (84.19%), Postives = 244/272 (89.71%), Query Frame = 1


Query: 8   FLIWFSAAMGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSA 67
           F IW S AM ILSAFLFPPPPSTFVNVMTVVSST++ MAG+SEIKGKHLQYSKFWNANS 
Sbjct: 3   FPIWVSVAMDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSG 62

Query: 68  PKAPSEEPKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVL 127
            K  ++E KLSSKYGMLL YTPAFLAGAASFW FPS +ER  +LK+ALTLHFFKRIFEVL
Sbjct: 63  SKLSAQESKLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVL 122

Query: 128 FIHKYSSKMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGN 187
           FIHKYSSKMV  TAI+ISFSY  ST IMIYTQHLSQGLPEPPIDLKNIG+ LFL GIIGN
Sbjct: 123 FIHKYSSKMVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGN 182

Query: 188 FYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGT 247
           FYHH+LLS+TRK  ET YKIPKGGLFS VICPHYLFEII +FGFAFISQELYPLCFS GT
Sbjct: 183 FYHHYLLSQTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGT 242

Query: 248 AIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
           AIYLAGRSYATRKWY+SKFENFP+HVKALLPF
Sbjct: 243 AIYLAGRSYATRKWYISKFENFPNHVKALLPF 274

[Close]

BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: gi|778706250|ref|XP_004135052.2| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus])

HSP 1 Score: 461.1 bits (1185), Expect = 2.9e-126
Identity = 225/275 (81.82%), Postives = 247/275 (89.82%), Query Frame = 1


Query: 5   LAVFLIWFSAAMGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNA 64
           L +F IW SAAM +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEI GKHLQYSKFWNA
Sbjct: 10  LKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNA 69

Query: 65  NSAPKAPSEEPKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIF 124
           N A K  +EE K+SSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIF
Sbjct: 70  NGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIF 129

Query: 125 EVLFIHKYSSKMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGI 184
           EVLFIHKYSSKMV  TAI+IS SY  ST IMIYTQHLSQGLPEPPIDLKNIG+  FLIGI
Sbjct: 130 EVLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGI 189

Query: 185 IGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFS 244
           IGNFYHH+LLS+TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS
Sbjct: 190 IGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFS 249

Query: 245 TGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
            GTA+YLAGRSYATRKWY+SKFE FP++VKALLPF
Sbjct: 250 IGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPF 284

[Close]

BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: gi|700196982|gb|KGN52159.1| (hypothetical protein Csa_5G612880 [Cucumis sativus])

HSP 1 Score: 422.2 bits (1084), Expect = 1.5e-114
Identity = 211/275 (76.73%), Postives = 230/275 (83.64%), Query Frame = 1


Query: 5   LAVFLIWFSAAMGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNA 64
           L +F IW SAAM +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEI GKHLQYSKFWNA
Sbjct: 10  LKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNA 69

Query: 65  NSAPKAPSEEPKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIF 124
           N A K  +EE K+SSKYGMLLAYTPAFLAGAASFW FPS +ER                 
Sbjct: 70  NGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDER----------------- 129

Query: 125 EVLFIHKYSSKMVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGI 184
            VLFIHKYSSKMV  TAI+IS SY  ST IMIYTQHLSQGLPEPPIDLKNIG+  FLIGI
Sbjct: 130 -VLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGI 189

Query: 185 IGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFS 244
           IGNFYHH+LLS+TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS
Sbjct: 190 IGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFS 249

Query: 245 TGTAIYLAGRSYATRKWYVSKFENFPSHVKALLPF 280
            GTA+YLAGRSYATRKWY+SKFE FP++VKALLPF
Sbjct: 250 IGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPF 266

[Close]

BLAST of Cp4.1LG01g14270 vs. NCBI nr
Match: gi|1021047852|gb|KZN05632.1| (hypothetical protein DCAR_006469 [Daucus carota subsp. sativus])

HSP 1 Score: 414.5 bits (1064), Expect = 3.1e-112
Identity = 232/558 (41.58%), Postives = 330/558 (59.14%), Query Frame = 1


Query: 19  LSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEPKLS 78
           L  F++PPP S F+N MTV++   +   G+ E+KGK +QYSKF+N +      S+  +LS
Sbjct: 6   LLRFIYPPPASWFINAMTVITGAILVNGGLMEVKGKSMQYSKFYNTSKKNAEASKITELS 65

Query: 79  SKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKMVF 138
            + GML+ Y PAF+A      F   +  R  ++ +ALT+HFFKR+FEV FIH YS  M  
Sbjct: 66  GRKGMLVVYAPAFVASVIFLAFMRDEGLRFTLVTSALTVHFFKRLFEVSFIHNYSGSMPI 125

Query: 139 DTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTR 198
           D  I  S SY   TV + Y+QHL   LPEP IDLK  G A FL+GI GNFYHH+LLSK R
Sbjct: 126 DIVINFSASYFLYTVTVTYSQHLVAELPEPVIDLKYAGTASFLVGITGNFYHHYLLSKLR 185

Query: 199 KPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYAT 258
           K GE  YKIP+G      +C           G ++ +++                     
Sbjct: 186 KEGEKQYKIPQG-----AVC---------LMGRSYATRKW-------------------- 245

Query: 259 RKWYVSKFENFPSHVKALLPFSDEAIVQRRRPPPPPPPPPPPSSGPSPMPTFLFSPFSST 318
              Y+SKFE+FP  VKAL+P    A+V                   S +  F++ P +S 
Sbjct: 246 ---YLSKFEDFPKDVKALIP----AMVL------------------STLLRFIYPPPASW 305

Query: 319 FLLALSSLTMLSILVLATSEFLGRHLQYSKFWSPK------TSSNKIHLPSRSAMLLCYV 378
           F+ A++ +   S++     E  G+ +QYSKF++        + +  I L  R+ ML+ Y 
Sbjct: 306 FITAMTVINGASLVNAGLMEIKGKSMQYSKFFNTNKKNVEASGAKIIELSGRNGMLVAYG 365

Query: 379 PAFAVASISLFLLLLPRYNNSSTRIFLINSLLLIHFFKRIFEVLFVHKYSNKMGLSDAIR 438
           PAF  +  SL  +      N   R  L++S L +HFFKR+FEVLF+HKYS  M +  AI 
Sbjct: 366 PAFVASVTSLAFM-----PNEGLRFTLVSSALAVHFFKRLFEVLFIHKYSGSMPIDSAIV 425

Query: 439 VSITYVLLTASTISSQMVSHNLEEPQIDLRAAGLAMYWIGITGNLYHHVLLAKLRKEGDR 498
           +S +Y L +  TI SQ +     EP +DL+ AG+ ++ +GI GN YHH LL+KLRKEGD+
Sbjct: 426 ISTSYFLSSLCTIYSQHLCGQFPEPVVDLKYAGITIFLVGIVGNFYHHYLLSKLRKEGDK 485

Query: 499 EYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFPMVFAFGSALFLIGRSYATRNWYV 558
           EY+IP+GG+F++++CPHYLFE++ F+GI+ +SQT++ + F  G+  +L GRSYATR WY+
Sbjct: 486 EYKIPQGGLFDLVICPHYLFEILGFIGISCMSQTVYALSFTAGTIGYLTGRSYATRRWYL 499

Query: 559 SKFEDFPRHVKAILPFVF 571
           SKFEDFP+ VKA++P++F
Sbjct: 546 SKFEDFPKDVKALIPYIF 499

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g14270 vs. Swiss-Prot


Match Name	E-value	Identity	Description
S5A1_MACFA	5.8e-18	41.90	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV...	[more]
S5A1_MOUSE	9.8e-18	29.15	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2	[more]
S5A1_RAT	1.3e-17	43.27	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2	[more]
S5A1_HUMAN	1.3e-17	31.98	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1	[more]
S5A1_BOVIN	8.3e-17	31.82	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus GN=SRD5A1 PE=2 SV=1	[more]

BLAST of Cp4.1LG01g14270 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A103Y9N2_CYNCS	3.3e-137	49.19	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal OS=Cynara cardunculus var. sco...	[more]
A0A0A0KTC7_CUCSA	1.0e-114	76.73	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612880 PE=4 SV=1	[more]
A0A166DSU9_DAUCA	2.1e-112	41.58	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_006469 PE=4 SV=1	[more]
A0A0A0KWK8_CUCSA	1.8e-103	72.45	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612900 PE=4 SV=1	[more]
A0A0A0KRA7_CUCSA	5.0e-101	69.26	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612890 PE=4 SV=1	[more]

BLAST of Cp4.1LG01g14270 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16010.1	9.9e-85	56.77	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	2.0e-13	37.74	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

BLAST of Cp4.1LG01g14270 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|976920344\|gb\|KVI05075.1\|	4.7e-137	49.19	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal [Cynara cardunculus var. scoly...	[more]
gi\|659091769\|ref\|XP_008446722.1\|	1.5e-127	84.19	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo]	[more]
gi\|778706250\|ref\|XP_004135052.2\|	2.9e-126	81.82	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus]	[more]
gi\|700196982\|gb\|KGN52159.1\|	1.5e-114	76.73	hypothetical protein Csa_5G612880 [Cucumis sativus]	[more]
gi\|1021047852\|gb\|KZN05632.1\|	3.1e-112	41.58	hypothetical protein DCAR_006469 [Daucus carota subsp. sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Vocabulary: **INTERPRO**
Term	Definition
IPR001104	3-oxo-5_a-steroid_4-DH_C

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g14270.1	Cp4.1LG01g14270.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 463..570 score: 9.9E-28coord: 140..279 score: 2.4
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROFILE	PS50244	S5A_REDUCTASE	coord: 176..243 score: 25.788coord: 465..534 score: 2
None	No IPR available	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 523..570 score: 3.8E-111coord: 31..233 score: 3.8E
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 31..233 score: 3.8E-111coord: 523..570 score: 3.8E
None	No IPR available	unknown	SSF101447	Formin homology 2 domain (FH2 domain)	coord: 385..421 score: 2.2E-5coord: 290..300 score: 2.

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG01g14270	Silver-seed gourd	carcpeB1177
Cp4.1LG01g14270	Silver-seed gourd	carcpeB1466
Cp4.1LG01g14270	Cucumber (Chinese Long) v3	cpecucB0543
Cp4.1LG01g14270	Wax gourd	cpewgoB0563
Cp4.1LG01g14270	Cucurbita pepo (Zucchini)	cpecpeB040
Cp4.1LG01g14270	Cucumber (Gy14) v1	cgycpeB0750
Cp4.1LG01g14270	Cucurbita maxima (Rimu)	cmacpeB429
Cp4.1LG01g14270	Cucurbita maxima (Rimu)	cmacpeB720
Cp4.1LG01g14270	Cucurbita moschata (Rifu)	cmocpeB392
Cp4.1LG01g14270	Cucurbita moschata (Rifu)	cmocpeB673
Cp4.1LG01g14270	Wild cucumber (PI 183967)	cpecpiB439
Cp4.1LG01g14270	Cucumber (Chinese Long) v2	cpecuB439
Cp4.1LG01g14270	Bottle gourd (USVL1VR-Ls)	cpelsiB352
Cp4.1LG01g14270	Watermelon (Charleston Gray)	cpewcgB388
Cp4.1LG01g14270	Watermelon (97103) v1	cpewmB427
Cp4.1LG01g14270	Melon (DHL92) v3.5.1	cpemeB363
Cp4.1LG01g14270	Cucumber (Gy14) v2	cgybcpeB648
Cp4.1LG01g14270	Melon (DHL92) v3.6.1	cpemedB423