Cp4.1LG01g12640 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG01g12640
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectate lyase
LocationCp4.1LG01 : 8663789 .. 8666488 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAAAGCAACGGAAACAGAAAAAACAGCCAAAGCTGAGCCACAGAAAATATCTATGTTAGTAAATAACTGTTTTAACCGTCCGAGTAGCTAACGGTTCTCCCTTCTTCCCGCCATGAACGACCTTCGCTACCCCATCATCATCTTCCTCTGTTTTCCCTCATAACTCCTTAAAACCTTCTCCTTCCCATCAACCAATTCACACTTCCTCAAGCGGAATCCTTCGTGTTTCGCTTGAGGATTCAAATCCGGTAATCATAGTCTAAGATCCAAAGGGCTTTCATCATCCCACAGAAATGGTAGCACCCACGAGCAGAGCTATGCTTGGGGTTCTGCTAACATTATCCTTGGTGGCTACTGCATATGCTGACATTGGAAACTTCGATGATTACTGGAAATCACGCGCCGCCAAGGCTGAGAAAGCCGCCGCCAAGGCCTTCGACCCTAATCCAGAGAACGTCACCGACATCCTCAATGCTGAAGTTGAGCAGTAAGTTGTTTTATTTTTTTTTTTTCAATCTTCAGGAGAAATTTTGTAGTAAGAACTCAGGGGAAATGAATTAATGGGATTGGAGTTTGATGCAGGGAATTGAGCGGAAGCAATGATACGAGAAGGAATTTGAAGAGATATAAAGGGCCATGTCTAGCCACTAATCCCATTGACCGGTGCTGGAGATGCGACCCCAATTGGGCAAAAAACAGGAAGAAGCTGGCCGATTGTGTTCTGGGTTTTGGCTGGAAGACCATCGGCGGGAAGTTGGGTCCATTCTACGTGGTGAACGATTCGTCGGACTCGGATATCATGGAACCAAAACCTGGGACCCTCCGACATGCTGTGATCCAAAAGGGGCCGTTGTGGATCACATTCTCAACCAACATGGCCATTAGGCTAACTCAGGAGCTCATCGTGTCAAGCGACAAGACCATAGATGCAAGAGGCGCTAACGTTCAGATTGCTTATGGGGCTGGCATTACTCTTCAGTATGTACAAAATGTGATCATCCATGGGCTTCGTATCCATCACATCGTCATGGGTAGCGGCGGAATGATCAGGGACGCTGTGGACCATGTGGGTTTGAGGACAATGAGTGACGGCGATGGCATTTCAATTTTCGGTTCCTCCAACATTTGGATTGACCATGTTTCCATGTCCAATTGTCAAGATGGTCTAATCGATGCCATCATGGGATCCACTGCCATCACCATATCCAATAGCCATTTCACTCACCACAATGATGTAATGAAGCATGCTGAAACCATGTGACTGATGTTTTCATAAAAGAGTTTAAAAGTTGGGAGTTTTATGCAGGTGATGCTGTTCGGAGCAAGCGATGGGTACTCGAATGATCAGATAATGCAAGTGACAGTGGCATTCAATCACTTTGGTCAAGGGTTGGTGCAGAGGATGCCAAGGTGCCGATGGGGGTTCTTCCATGTCGTCAACAATGACTACACTCATTGGCTTATGTATGCCATAGGTGGTAGCCAGCATCCCATCATTATCAGCCAGGGCAATCGATTCATTGCTCCTCCAAATGTAAATGCTAAAGAGGTAATCAAATCAAATCAAATCAAATCAAATATTTAATTTTCTCAAGAAACTAAAACTGGGTGTGAACCAACTCAGGTGACAAAGAGAGAGTACTCGCCGGAGAGTGTGTGGAAGACGTGGACATGGAGATCAGAGGGAGACTTGATGATGAACGGAGCATTCTTTGTTACCTCCGGCGACCAGAGGAAGAAACGACCTTTCTCAAAGATGGATATGATCTCGTACAGAGAAGGGACATACGTTACCAGAATGACACGTATGTCAGGTGCGCTAGCCTGCTTCATCGGCAGACCATGCTAAACTTCAACTCACACGTCGACTTAAACTCGCCGTCAACGAACAGATTTTTACTCTTTGGGTCACCGCTCAACTCATAGAGTATTAATTTTTTGGTTGGCTCGACTGGATTGGGTCTTTCTGGCATTATCAACCCTCTTTGTTCACTTCCTTATTTACCATAGCATTAATAAATATTTGTTATTATTGGACATTTTTTTTATGGGGAAATTTTGAAAGAATACAAATGTGAAATAAAAGAACGTAAATTTGACGTGCGTTCTAACACATAATGTTACAACTTTTCTTTTTTATTTTTACCTTTGGCAAAGTCTTAAAAATTCCTCCTGTAAATGTTGCAAACTGTAAAAAAAAAAAAAAAAGATGTTAGAACTTTTATTTTACCTTGGCAAGATCATAAAAATTCCTCCTGTAAATGTTGTGCACTGTAAAAAAAAAAAAAAAAAGTGAGCTACAAAAGAATGGATGATGAATGAACAGTGATTCTAAAGAAATAATCAGCCACAAGAAGCGACATAGTAGTGATTTACGGGGCAAAATGGGGCAAAATTTTGTTCCATGACCCTTACGTATCATTGATTCTGTAGAATCTGGAGTGGAATTGAATTTCTCAATACTGTAGTTTATTCGTGTGAAGGAGTAATCACACCTCAAAAAGTCCTAACGTTGCATGGTATTTTACTCATTGCCAACATGAACTATTCATAAATTTTAGGAAAAGGAAGAAATTGCGCCTCCTTGGAATATATGTAAGATAAAAAACTTGAACAAAAGTGTGGATGATGGTGGAATCGACACAGGAGGATGCACAGCAGACAATCTCTCTTCCCATTAACATCTAGTTGATGCGGA

mRNA sequence

TTAAAAGCAACGGAAACAGAAAAAACAGCCAAAGCTGAGCCACAGAAAATATCTATGTTAGTAAATAACTGTTTTAACCGTCCGAGTAGCTAACGGTTCTCCCTTCTTCCCGCCATGAACGACCTTCGCTACCCCATCATCATCTTCCTCTGTTTTCCCTCATAACTCCTTAAAACCTTCTCCTTCCCATCAACCAATTCACACTTCCTCAAGCGGAATCCTTCGTGTTTCGCTTGAGGATTCAAATCCGGTAATCATAGTCTAAGATCCAAAGGGCTTTCATCATCCCACAGAAATGGTAGCACCCACGAGCAGAGCTATGCTTGGGGTTCTGCTAACATTATCCTTGGTGGCTACTGCATATGCTGACATTGGAAACTTCGATGATTACTGGAAATCACGCGCCGCCAAGGCTGAGAAAGCCGCCGCCAAGGCCTTCGACCCTAATCCAGAGAACGTCACCGACATCCTCAATGCTGAAGTTGAGCAGGAATTGAGCGGAAGCAATGATACGAGAAGGAATTTGAAGAGATATAAAGGGCCATGTCTAGCCACTAATCCCATTGACCGGTGCTGGAGATGCGACCCCAATTGGGCAAAAAACAGGAAGAAGCTGGCCGATTGTGTTCTGGGTTTTGGCTGGAAGACCATCGGCGGGAAGTTGGGTCCATTCTACGTGGTGAACGATTCGTCGGACTCGGATATCATGGAACCAAAACCTGGGACCCTCCGACATGCTGTGATCCAAAAGGGGCCGTTGTGGATCACATTCTCAACCAACATGGCCATTAGGCTAACTCAGGAGCTCATCGTGTCAAGCGACAAGACCATAGATGCAAGAGGCGCTAACGTTCAGATTGCTTATGGGGCTGGCATTACTCTTCAGTATGTACAAAATGTGATCATCCATGGGCTTCGTATCCATCACATCGTCATGGGTAGCGGCGGAATGATCAGGGACGCTGTGGACCATGTGGGTTTGAGGACAATGAGTGACGGCGATGGCATTTCAATTTTCGGTTCCTCCAACATTTGGATTGACCATGTTTCCATGTCCAATTGTCAAGATGGTCTAATCGATGCCATCATGGGATCCACTGCCATCACCATATCCAATAGCCATTTCACTCACCACAATGATGTGATGCTGTTCGGAGCAAGCGATGGGTACTCGAATGATCAGATAATGCAAGTGACAGTGGCATTCAATCACTTTGGTCAAGGGTTGGTGCAGAGGATGCCAAGGTGCCGATGGGGGTTCTTCCATGTCGTCAACAATGACTACACTCATTGGCTTATGTATGCCATAGGTGGTAGCCAGCATCCCATCATTATCAGCCAGGGCAATCGATTCATTGCTCCTCCAAATGTAAATGCTAAAGAGGTGACAAAGAGAGAGTACTCGCCGGAGAGTGTGTGGAAGACGTGGACATGGAGATCAGAGGGAGACTTGATGATGAACGGAGCATTCTTTGTTACCTCCGGCGACCAGAGGAAGAAACGACCTTTCTCAAAGATGGATATGATCTCGTACAGAGAAGGGACATACGTTACCAGAATGACACGTATGTCAGGTGCGCTAGCCTGCTTCATCGGCAGACCATGCTAAACTTCAACTCACACGTCGACTTAAACTCGCCGTCAACGAACAGATTTTTACTCTTTGGGTCACCGCTCAACTCATAGAGTATTAATTTTTTGGTTGGCTCGACTGGATTGGGTCTTTCTGGCATTATCAACCCTCTTTGTTCACTTCCTTATTTACCATAGCATTAATAAATATTTGTTATTATTGGACATTTTTTTTATGGGGAAATTTTGAAAGAATACAAATGTGAAATAAAAGAACGTAAATTTGACGTGCGTTCTAACACATAATGTTACAACTTTTCTTTTTTATTTTTACCTTTGGCAAAGTCTTAAAAATTCCTCCTGTAAATGTTGCAAACTGTAAAAAAAAAAAAAAAAGATGTTAGAACTTTTATTTTACCTTGGCAAGATCATAAAAATTCCTCCTGTAAATGTTGTGCACTGTAAAAAAAAAAAAAAAAAGTGAGCTACAAAAGAATGGATGATGAATGAACAGTGATTCTAAAGAAATAATCAGCCACAAGAAGCGACATAGTAGTGATTTACGGGGCAAAATGGGGCAAAATTTTGTTCCATGACCCTTACGTATCATTGATTCTGTAGAATCTGGAGTGGAATTGAATTTCTCAATACTGTAGTTTATTCGTGTGAAGGAGTAATCACACCTCAAAAAGTCCTAACGTTGCATGGTATTTTACTCATTGCCAACATGAACTATTCATAAATTTTAGGAAAAGGAAGAAATTGCGCCTCCTTGGAATATATGTAAGATAAAAAACTTGAACAAAAGTGTGGATGATGGTGGAATCGACACAGGAGGATGCACAGCAGACAATCTCTCTTCCCATTAACATCTAGTTGATGCGGA

Coding sequence (CDS)

ATGGTAGCACCCACGAGCAGAGCTATGCTTGGGGTTCTGCTAACATTATCCTTGGTGGCTACTGCATATGCTGACATTGGAAACTTCGATGATTACTGGAAATCACGCGCCGCCAAGGCTGAGAAAGCCGCCGCCAAGGCCTTCGACCCTAATCCAGAGAACGTCACCGACATCCTCAATGCTGAAGTTGAGCAGGAATTGAGCGGAAGCAATGATACGAGAAGGAATTTGAAGAGATATAAAGGGCCATGTCTAGCCACTAATCCCATTGACCGGTGCTGGAGATGCGACCCCAATTGGGCAAAAAACAGGAAGAAGCTGGCCGATTGTGTTCTGGGTTTTGGCTGGAAGACCATCGGCGGGAAGTTGGGTCCATTCTACGTGGTGAACGATTCGTCGGACTCGGATATCATGGAACCAAAACCTGGGACCCTCCGACATGCTGTGATCCAAAAGGGGCCGTTGTGGATCACATTCTCAACCAACATGGCCATTAGGCTAACTCAGGAGCTCATCGTGTCAAGCGACAAGACCATAGATGCAAGAGGCGCTAACGTTCAGATTGCTTATGGGGCTGGCATTACTCTTCAGTATGTACAAAATGTGATCATCCATGGGCTTCGTATCCATCACATCGTCATGGGTAGCGGCGGAATGATCAGGGACGCTGTGGACCATGTGGGTTTGAGGACAATGAGTGACGGCGATGGCATTTCAATTTTCGGTTCCTCCAACATTTGGATTGACCATGTTTCCATGTCCAATTGTCAAGATGGTCTAATCGATGCCATCATGGGATCCACTGCCATCACCATATCCAATAGCCATTTCACTCACCACAATGATGTGATGCTGTTCGGAGCAAGCGATGGGTACTCGAATGATCAGATAATGCAAGTGACAGTGGCATTCAATCACTTTGGTCAAGGGTTGGTGCAGAGGATGCCAAGGTGCCGATGGGGGTTCTTCCATGTCGTCAACAATGACTACACTCATTGGCTTATGTATGCCATAGGTGGTAGCCAGCATCCCATCATTATCAGCCAGGGCAATCGATTCATTGCTCCTCCAAATGTAAATGCTAAAGAGGTGACAAAGAGAGAGTACTCGCCGGAGAGTGTGTGGAAGACGTGGACATGGAGATCAGAGGGAGACTTGATGATGAACGGAGCATTCTTTGTTACCTCCGGCGACCAGAGGAAGAAACGACCTTTCTCAAAGATGGATATGATCTCGTACAGAGAAGGGACATACGTTACCAGAATGACACGTATGTCAGGTGCGCTAGCCTGCTTCATCGGCAGACCATGCTAA

Protein sequence

MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALACFIGRPC
BLAST of Cp4.1LG01g12640 vs. Swiss-Prot
Match: PLY59_SOLLC (Probable pectate lyase P59 OS=Solanum lycopersicum GN=LAT59 PE=2 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 3.3e-162
Identity = 275/432 (63.66%), Postives = 326/432 (75.46%), Query Frame = 1

Query: 17  SLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQEL--------S 76
           +++ +  A IG++D+ W+ RA +A++ A   ++P+PENVT   N ++   +        +
Sbjct: 19  TIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMKELKKVKGT 78

Query: 77  GSNDTRRNL--KRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPF 136
            +N TRR L  K+Y GPC+ TNPID+CWRCDPNWA NRKKLADC +GFG K IGGK G F
Sbjct: 79  HNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKAIGGKDGEF 138

Query: 137 YVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANV 196
           YVV D+SD D  +PKPGTLRHAVIQK PLWI F   M IRL QE+I+ SDKTIDARG NV
Sbjct: 139 YVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKTIDARGVNV 198

Query: 197 QIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNI 256
            I  GAGITLQY++NVIIHGL IH IV G+GGM+RDAVDH+G+RT SDGDGISIFG+S I
Sbjct: 199 HITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGISIFGASYI 258

Query: 257 WIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNH 316
           WIDHVSM  C DGLIDA+ GST ITISN HFT HN+VMLFGASD  S DQ+MQ+T+AFNH
Sbjct: 259 WIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVMQITLAFNH 318

Query: 317 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTK 376
           FG+ L+QRMPRCRWG+ HVVNNDYTHW MYAIGGS HP II QGNRFIAPP++  K+VTK
Sbjct: 319 FGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPDIFKKQVTK 378

Query: 377 REYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKM-DMISYREGTYVTRMTRM 436
           REY+PESVW  WTWRSEG+L MNGA+F  SGD         + D IS      VT MTR 
Sbjct: 379 REYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPAEDVTWMTRF 438

Query: 437 SGALACFIGRPC 438
           +G L C  G+PC
Sbjct: 439 AGVLGCKPGKPC 449

BLAST of Cp4.1LG01g12640 vs. Swiss-Prot
Match: PLY_LILLO (Pectate lyase OS=Lilium longiflorum PE=2 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 5.2e-152
Identity = 263/426 (61.74%), Postives = 314/426 (73.71%), Query Frame = 1

Query: 13  LLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSND 72
           LL  +  A + A+I  FD+YW+ ++  A+  A KA+ P+PE VT+  N  V     G N 
Sbjct: 11  LLFFASAALSSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NS 70

Query: 73  TRRNLKRYK-GPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVND 132
           TRRNL+  K G CLATNPIDRCWRC  NW+ NRK L  CV GFG KT GG  G  YVV D
Sbjct: 71  TRRNLRTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTD 130

Query: 133 SSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYG 192
            SD  + +PK GTLR  VIQ  PLWI F  +M IRL QELI+++DKTID RGANVQIA G
Sbjct: 131 PSDDSLTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGG 190

Query: 193 AGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWIDHV 252
           A +T+Q+V NVIIHG+ IH I  G GG+IRD+  H G+RT SDGDGISI GSSNIWIDHV
Sbjct: 191 AQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHV 250

Query: 253 SMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGL 312
           S++ C DGLID I+GSTAITISN H T H+DVML GASD Y+ D+IMQVTVAFNHFG+GL
Sbjct: 251 SLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGL 310

Query: 313 VQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSP 372
           VQRMPRCR+GF HVVNNDYTHW+MYA+GGSQHP IISQGNR+IAP    AKEVTKR+Y+ 
Sbjct: 311 VQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAE 370

Query: 373 ESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALAC 432
            + W  WTW+S+GDL ++GAFFV SG   + + +SK D+I  + GT+V R+TR SGAL C
Sbjct: 371 PAEWSKWTWKSQGDLFVSGAFFVESGGPFENK-YSKKDLIKAKPGTFVQRLTRFSGALNC 430

Query: 433 FIGRPC 438
                C
Sbjct: 431 KENMEC 434

BLAST of Cp4.1LG01g12640 vs. Swiss-Prot
Match: PLY7_ARATH (Probable pectate lyase 7 OS=Arabidopsis thaliana GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.1e-149
Identity = 260/464 (56.03%), Postives = 329/464 (70.91%), Query Frame = 1

Query: 12  VLLTLSLVATAYADIGN-FDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDI 71
           V+   SL+ T  A++ +  D+YW ++A +A K    A+ P+P            +N TD+
Sbjct: 12  VICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDV 71

Query: 72  LNAEVEQEL------------SGSNDTRRNL------------KRYKGPCLATNPIDRCW 131
              E E+ +            S +N TRR+L             +  GPC A+NPID+CW
Sbjct: 72  EGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCW 131

Query: 132 RCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGP 191
           RC P+WA+ RKKL  CV GFG++T GGK G  YVV    D D++ P+PGTLRHAVIQK P
Sbjct: 132 RCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEP 191

Query: 192 LWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVM 251
           LWI F  +M+IRL+QEL+++SDKTIDARGANV IAYGAGIT+QYV N+IIHGL +HHIV 
Sbjct: 192 LWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVK 251

Query: 252 GSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISN 311
            SGG+IRD+++H G R  +DGDGISIFG++NIW+DH+SMS CQDGLIDAIMGSTAITISN
Sbjct: 252 SSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISN 311

Query: 312 SHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 371
           SHFTHHNDVML GA +   +D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW 
Sbjct: 312 SHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWE 371

Query: 372 MYAIGGSQHPIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFF 431
           +YAIGGSQ P I+S GNRFIAPP+  + +EVTKR+Y+ ES WK W WRSE D+ MN A+F
Sbjct: 372 LYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYF 431

Query: 432 VTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALACFIGRPC 438
             SG+   K   S+  MI  + G  V+++T+ +GAL C +G+ C
Sbjct: 432 RQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Cp4.1LG01g12640 vs. Swiss-Prot
Match: PLY19_ARATH (Probable pectate lyase 19 OS=Arabidopsis thaliana GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.6e-148
Identity = 257/455 (56.48%), Postives = 322/455 (70.77%), Query Frame = 1

Query: 18  LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDILNAE--- 77
           L+ T   +I   D+YW  RA +A +   +A+  +P            +N TD+   E   
Sbjct: 18  LIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDG 77

Query: 78  -----------VEQELSGSNDTRRNLK--------RYKGPCLATNPIDRCWRCDPNWAKN 137
                      +E   S +N TRR+L+        + KGPC A+NPID+CWRC  +WAK 
Sbjct: 78  DAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKR 137

Query: 138 RKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNM 197
           RKKL  CV GFG +T GGK G  YVV  + D D++ PKPGTLRHAVIQK PLWI F  +M
Sbjct: 138 RKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDM 197

Query: 198 AIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDA 257
           +IRL QEL+++S KTIDARGANV +A+GAGIT+Q+V+NVIIHGL IHHI   SGGMIRD+
Sbjct: 198 SIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHISESSGGMIRDS 257

Query: 258 VDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDV 317
           VDH G+RT +DGDG+SI+GSSNIW+DH+SMS CQDGLIDAI+GST ITISNSHFTHHNDV
Sbjct: 258 VDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDV 317

Query: 318 MLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 377
           ML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIGGSQ 
Sbjct: 318 MLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQG 377

Query: 378 PIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKK 437
           P I+S GNRFIAPP+  + +EVTKR+Y+ E  WK W WRS+ D+ MNGA+F  SG+ + K
Sbjct: 378 PTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYK 437

BLAST of Cp4.1LG01g12640 vs. Swiss-Prot
Match: PLY3_ARATH (Probable pectate lyase 3 OS=Arabidopsis thaliana GN=AT59 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 4.6e-148
Identity = 253/430 (58.84%), Postives = 310/430 (72.09%), Query Frame = 1

Query: 25  DIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGS---NDTRRNLKRYK 84
           ++  FD YW  R + A K    ++DP+P NVT+  N  V   +  S   NDTRR L + +
Sbjct: 30  NVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQVR 89

Query: 85  ---------GPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDS 144
                    G CLA NPID CWRCD NWA NRKKLADCVLGFG +T GGK GP YVV D+
Sbjct: 90  SGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKDA 149

Query: 145 SDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGA 204
           SD+D++ PKPGTLRHAV + GPLWI F+ +M I+L QEL+++SDKTID RGA V I  GA
Sbjct: 150 SDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEGA 209

Query: 205 GITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWIDHVS 264
           G+TLQ+V NVIIH + + HIV G+GG+IRD+  H+GLRT SDGDGIS+FG++NIWIDHVS
Sbjct: 210 GLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHVS 269

Query: 265 MSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLV 324
           M+ C DG+IDAI GSTA+TISNSHFT H +VMLFGA D +  D+ MQ+TVAFNHFG+ L 
Sbjct: 270 MTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRLE 329

Query: 325 QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPE 384
           QRMPRCR+G  HVVNNDYTHW MYAIGG+ +P IISQGNRFIAPPN  AK++TKREY+P 
Sbjct: 330 QRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTPY 389

Query: 385 SVWKTWTWRSEGDLMMNGAFFVTSG-----DQRKKRPFSKMDMISYREGTYVTRMTRMSG 438
             WK+W W+SEGD  +NGA+FV SG       + K P      I  + GT V ++T  +G
Sbjct: 390 GEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLTMDAG 449

BLAST of Cp4.1LG01g12640 vs. TrEMBL
Match: A0A0A0KSL4_CUCSA (Pectate lyase OS=Cucumis sativus GN=Csa_5G604340 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 1.6e-224
Identity = 370/429 (86.25%), Postives = 401/429 (93.47%), Query Frame = 1

Query: 10  LGVLLTLSLVAT-AYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELS 69
           L +LLTLSL+AT A+ADIG+FDDYWK+RAA AEKAAA A+DP+PE V + +NAE+    S
Sbjct: 4   LSLLLTLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYDPDPETVANSVNAEIAHASS 63

Query: 70  GSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYV 129
           G N TRRNLK+Y GPCLATNPIDRCWRCDPNWA+NRKKLADCVLGFG KT GGKLGP+YV
Sbjct: 64  GRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYV 123

Query: 130 VNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQI 189
           VNDSSDSD+M PKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI++SDKTIDARGANVQI
Sbjct: 124 VNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQI 183

Query: 190 AYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWI 249
           AYGAGITLQY++NVIIHGLRIHHIV+GSGGMIRDAVDHVGLRTMSDGDGISIFGSSN+WI
Sbjct: 184 AYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWI 243

Query: 250 DHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFG 309
           DHVSMSNC DGLIDAIMGSTAITISN HFTHHN+VMLFGASDGYS DQIMQ+TVAFNHFG
Sbjct: 244 DHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQITVAFNHFG 303

Query: 310 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKRE 369
           QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTKRE
Sbjct: 304 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTKRE 363

Query: 370 YSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGA 429
           YSPE VWK+WTWRSEGDLMMNGAFFVTSGDQ K+RPFS+MDMISY+ GTYV RMTR +G+
Sbjct: 364 YSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVKRMTRFAGS 423

Query: 430 LACFIGRPC 438
           LACF+GRPC
Sbjct: 424 LACFVGRPC 432

BLAST of Cp4.1LG01g12640 vs. TrEMBL
Match: A0A059BFL8_EUCGR (Pectate lyase OS=Eucalyptus grandis GN=EUGRSUZ_G02541 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 2.3e-191
Identity = 314/420 (74.76%), Postives = 361/420 (85.95%), Query Frame = 1

Query: 18  LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSNDTRRNL 77
           LV +  A+I  FD+YW  RA +A+K A KA+DP+PE VT + NA+V++ + G+N TRR L
Sbjct: 17  LVPSLQANIEEFDEYWAKRAEEAQKDALKAYDPHPETVTSLFNAKVDKAVRGNNSTRRQL 76

Query: 78  KRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDI 137
           K+YKGPCLATNPIDRCWRC+ NWA+NRK+LA+CVLGFG KT GGK G FYVV DSSD+D+
Sbjct: 77  KKYKGPCLATNPIDRCWRCNRNWARNRKRLANCVLGFGRKTRGGKRGWFYVVTDSSDNDM 136

Query: 138 MEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQ 197
           + PKPGTLRHAV+QKGPLWI F+ +M IRL QELI++SDKTIDARGANV IAYGAGITLQ
Sbjct: 137 VNPKPGTLRHAVVQKGPLWIIFAGSMVIRLNQELIMTSDKTIDARGANVHIAYGAGITLQ 196

Query: 198 YVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQ 257
           +++NVIIHGL IH IV GSGG+IRDA DHVGLRT SDGDGISIFGSS++WIDHVSMS C 
Sbjct: 197 FIRNVIIHGLHIHDIVRGSGGLIRDAHDHVGLRTASDGDGISIFGSSHLWIDHVSMSACS 256

Query: 258 DGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPR 317
           DGLIDAI GSTAITISN HFTHHN+VMLFGASDGYS DQIMQ+TVAFNHFG+GLVQRMPR
Sbjct: 257 DGLIDAIQGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQITVAFNHFGRGLVQRMPR 316

Query: 318 CRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWKT 377
           CRWGFFHVVNNDYTHWLMYAIGGSQHP IISQGNRFIAPPN+ AKEVTKR+Y+ E  WK 
Sbjct: 317 CRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNIAAKEVTKRDYATEDQWKN 376

Query: 378 WTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALACFIGRPC 437
           W W+SEGDLMMNGAFFV SG+ R + PFS+ D+I  + GTYV R+TR +G L C +GRPC
Sbjct: 377 WLWKSEGDLMMNGAFFVNSGNPRARFPFSRRDVIKAKPGTYVRRLTRFAGKLGCRVGRPC 436

BLAST of Cp4.1LG01g12640 vs. TrEMBL
Match: W9SK59_9ROSA (Pectate lyase OS=Morus notabilis GN=L484_001952 PE=3 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 9.5e-185
Identity = 304/437 (69.57%), Postives = 359/437 (82.15%), Query Frame = 1

Query: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MV  T   ++ ++  + L+ +  A+IG+FD+ W+ RA +A KAA +A+DPNPE VTD  N
Sbjct: 1   MVGTTKLGLVFLIGFVVLIPSLQANIGDFDESWQQRAEEAHKAALEAYDPNPEEVTDSFN 60

Query: 61  AEVEQELSGSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120
            EV++ + GSN TRR L+RY GPC ATNPIDRCWRC  NWA NRK+LA CVLGFG KT G
Sbjct: 61  EEVDKVIDGSNSTRRELRRYSGPCAATNPIDRCWRCRSNWASNRKRLARCVLGFGRKTTG 120

Query: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           G+ G +YVV D SD+D+  P+PGTLRHAVIQK PLWI F+ +M IRL+QEL+++S KTID
Sbjct: 121 GRSGRYYVVTDPSDNDVQNPRPGTLRHAVIQKQPLWIIFAHSMIIRLSQELLITSHKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISI 240
           ARGAN+ IA GAGIT+Q+ +NVIIHGL IH IV   GGMIRD+VDH G RT +DGDGISI
Sbjct: 181 ARGANIHIANGAGITIQFARNVIIHGLHIHDIVASGGGMIRDSVDHFGFRTPADGDGISI 240

Query: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGS+NIW+DH+SMSNCQDGLIDAI GSTAITISN HFTHHNDVMLFGASD +  D+IMQV
Sbjct: 241 FGSTNIWLDHISMSNCQDGLIDAIQGSTAITISNCHFTHHNDVMLFGASDSFEGDKIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFG+GLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGS HP IISQGNRFIAPPN  
Sbjct: 301 TVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSSHPTIISQGNRFIAPPNNA 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AK+VTKR+Y+ E  WK WTWRSEGDLMMNGAFFV SG++ KKRPF++ DMI  + GT+VT
Sbjct: 361 AKQVTKRDYAAEWEWKRWTWRSEGDLMMNGAFFVQSGNRSKKRPFTRHDMIKAKPGTFVT 420

Query: 421 RMTRMSGALACFIGRPC 438
           R+TR +GAL C+ G+ C
Sbjct: 421 RLTRFAGALNCYAGKKC 437

BLAST of Cp4.1LG01g12640 vs. TrEMBL
Match: W9S2X9_9ROSA (Pectate lyase OS=Morus notabilis GN=L484_014236 PE=3 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 2.8e-184
Identity = 305/420 (72.62%), Postives = 355/420 (84.52%), Query Frame = 1

Query: 22  AYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSNDTRRNLKRYK 81
           A+A I  FDDYWKSRA +A+K A +A+ P+PE VT+ LN +VE+ L+  N TRR L+  K
Sbjct: 2   AHAHIAEFDDYWKSRAEQAQKDALQAYHPHPEEVTNQLNYDVEEALTVKNSTRRVLRGKK 61

Query: 82  ----GPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDI 141
               GPC+ATNPIDRCWRCDPNWA NRK+LADC  GFG  ++GGK G +YVV DSSD+D+
Sbjct: 62  NKKGGPCMATNPIDRCWRCDPNWASNRKRLADCARGFGRNSVGGKFGRYYVVTDSSDTDL 121

Query: 142 MEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQ 201
           + PKPGTLRHAVIQK PLWI F+ +M IRL++ELI++ DKTID RGANV+IAYGAGIT+Q
Sbjct: 122 VNPKPGTLRHAVIQKAPLWIIFARSMIIRLSEELIMTGDKTIDGRGANVRIAYGAGITIQ 181

Query: 202 YVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQ 261
           +V+NVIIH + IH I  GSGG IRD+++H+G RT SDGDGISIFGSS+IWIDHVSMSNCQ
Sbjct: 182 FVKNVIIHNIHIHDINAGSGGNIRDSINHIGFRTKSDGDGISIFGSSHIWIDHVSMSNCQ 241

Query: 262 DGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPR 321
           DGLIDAIMGSTAITISN HFTHHN+VMLFGASD YS+DQ MQVTVAFNHFGQGLVQRMPR
Sbjct: 242 DGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYSDDQRMQVTVAFNHFGQGLVQRMPR 301

Query: 322 CRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWKT 381
           CRWGFFHVVNNDYTHWLMYAIGGSQHP IISQGNRFIAP ++NAK+VTKR+Y+PES WK 
Sbjct: 302 CRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPLDLNAKQVTKRDYAPESDWKN 361

Query: 382 WTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALACFIGRPC 438
           W WRSEGDL+MNGAFFV SG +  KR +S+  MI Y+ GT+VTR+TR +G L C IGRPC
Sbjct: 362 WRWRSEGDLLMNGAFFVQSGKRLSKRRYSRPGMIKYKPGTFVTRLTRYAGTLVCGIGRPC 421

BLAST of Cp4.1LG01g12640 vs. TrEMBL
Match: A0A0A0LCU3_CUCSA (Pectate lyase OS=Cucumis sativus GN=Csa_3G827350 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 3.0e-183
Identity = 300/430 (69.77%), Postives = 359/430 (83.49%), Query Frame = 1

Query: 12  VLLTLS-LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVE---QEL 71
           VL++++ L+ T  A I  FDD+   ++ +A++A+ +AF P+P NVTD  N  V    + +
Sbjct: 12  VLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFNQHVHLALEGI 71

Query: 72  SGSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFY 131
            GSN TRR+L +Y GPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFG KT GGK G  Y
Sbjct: 72  EGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKTTGGKKGRIY 131

Query: 132 VVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQ 191
           VV D SD+D++ P+PGTLR+  +QK PLWI F+ +M IRL++EL+++S KTIDARGANV 
Sbjct: 132 VVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSHKTIDARGANVH 191

Query: 192 IAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIW 251
           IAYGAG+++Q+ +NVIIHGLRIHH++   GGMIRD  +HVGLRT+SDGDGISIFGS+NIW
Sbjct: 192 IAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGISIFGSTNIW 251

Query: 252 IDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHF 311
           +DH+SMSNCQDGLIDAI GSTAITISNSHFTHHNDVMLFGASD Y  D IMQVTVAFNHF
Sbjct: 252 LDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHF 311

Query: 312 GQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKR 371
           G+GLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP IISQGNRFIAPPN  A+++T R
Sbjct: 312 GKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAARQITNR 371

Query: 372 EYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSG 431
            Y+ ESVWKTWTWRSEGDLMMNGA+FV SG+  K+RP+S+ DMI  + GT+V R+TR SG
Sbjct: 372 NYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSG 431

Query: 432 ALACFIGRPC 438
           +L C++GRPC
Sbjct: 432 SLNCYVGRPC 441

BLAST of Cp4.1LG01g12640 vs. TAIR10
Match: AT3G01270.1 (AT3G01270.1 Pectate lyase family protein)

HSP 1 Score: 531.2 bits (1367), Expect = 6.1e-151
Identity = 260/464 (56.03%), Postives = 329/464 (70.91%), Query Frame = 1

Query: 12  VLLTLSLVATAYADIGN-FDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDI 71
           V+   SL+ T  A++ +  D+YW ++A +A K    A+ P+P            +N TD+
Sbjct: 12  VICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDV 71

Query: 72  LNAEVEQEL------------SGSNDTRRNL------------KRYKGPCLATNPIDRCW 131
              E E+ +            S +N TRR+L             +  GPC A+NPID+CW
Sbjct: 72  EGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCW 131

Query: 132 RCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGP 191
           RC P+WA+ RKKL  CV GFG++T GGK G  YVV    D D++ P+PGTLRHAVIQK P
Sbjct: 132 RCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEP 191

Query: 192 LWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVM 251
           LWI F  +M+IRL+QEL+++SDKTIDARGANV IAYGAGIT+QYV N+IIHGL +HHIV 
Sbjct: 192 LWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVK 251

Query: 252 GSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISN 311
            SGG+IRD+++H G R  +DGDGISIFG++NIW+DH+SMS CQDGLIDAIMGSTAITISN
Sbjct: 252 SSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISN 311

Query: 312 SHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 371
           SHFTHHNDVML GA +   +D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW 
Sbjct: 312 SHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWE 371

Query: 372 MYAIGGSQHPIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFF 431
           +YAIGGSQ P I+S GNRFIAPP+  + +EVTKR+Y+ ES WK W WRSE D+ MN A+F
Sbjct: 372 LYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYF 431

Query: 432 VTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALACFIGRPC 438
             SG+   K   S+  MI  + G  V+++T+ +GAL C +G+ C
Sbjct: 432 RQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Cp4.1LG01g12640 vs. TAIR10
Match: AT5G15110.1 (AT5G15110.1 Pectate lyase family protein)

HSP 1 Score: 526.9 bits (1356), Expect = 1.2e-149
Identity = 256/455 (56.26%), Postives = 322/455 (70.77%), Query Frame = 1

Query: 18  LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDILNAE--- 77
           L+ T   +I   D+YW  RA +A +   +A+  +P            +N TD+   E   
Sbjct: 18  LIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDG 77

Query: 78  -----------VEQELSGSNDTRRNLK--------RYKGPCLATNPIDRCWRCDPNWAKN 137
                      +E   S +N TRR+L+        + KGPC A+NPID+CWRC  +WAK 
Sbjct: 78  DAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKR 137

Query: 138 RKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNM 197
           RKKL  CV GFG +T GGK G  YVV  + D D++ PKPGTLRHAVIQK PLWI F  +M
Sbjct: 138 RKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDM 197

Query: 198 AIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDA 257
           +IRL QEL+++S KTIDARGANV +A+GAGIT+Q+V+NV+IHGL IHHI   SGGMIRD+
Sbjct: 198 SIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHISESSGGMIRDS 257

Query: 258 VDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDV 317
           VDH G+RT +DGDG+SI+GSSNIW+DH+SMS CQDGLIDAI+GST ITISNSHFTHHNDV
Sbjct: 258 VDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDV 317

Query: 318 MLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 377
           ML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIGGSQ 
Sbjct: 318 MLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQG 377

Query: 378 PIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKK 437
           P I+S GNRFIAPP+  + +EVTKR+Y+ E  WK W WRS+ D+ MNGA+F  SG+ + K
Sbjct: 378 PTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYK 437

BLAST of Cp4.1LG01g12640 vs. TAIR10
Match: AT1G14420.1 (AT1G14420.1 Pectate lyase family protein)

HSP 1 Score: 525.8 bits (1353), Expect = 2.6e-149
Identity = 253/430 (58.84%), Postives = 310/430 (72.09%), Query Frame = 1

Query: 25  DIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGS---NDTRRNLKRYK 84
           ++  FD YW  R + A K    ++DP+P NVT+  N  V   +  S   NDTRR L + +
Sbjct: 30  NVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQVR 89

Query: 85  ---------GPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDS 144
                    G CLA NPID CWRCD NWA NRKKLADCVLGFG +T GGK GP YVV D+
Sbjct: 90  SGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKDA 149

Query: 145 SDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGA 204
           SD+D++ PKPGTLRHAV + GPLWI F+ +M I+L QEL+++SDKTID RGA V I  GA
Sbjct: 150 SDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEGA 209

Query: 205 GITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWIDHVS 264
           G+TLQ+V NVIIH + + HIV G+GG+IRD+  H+GLRT SDGDGIS+FG++NIWIDHVS
Sbjct: 210 GLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHVS 269

Query: 265 MSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLV 324
           M+ C DG+IDAI GSTA+TISNSHFT H +VMLFGA D +  D+ MQ+TVAFNHFG+ L 
Sbjct: 270 MTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRLE 329

Query: 325 QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPE 384
           QRMPRCR+G  HVVNNDYTHW MYAIGG+ +P IISQGNRFIAPPN  AK++TKREY+P 
Sbjct: 330 QRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTPY 389

Query: 385 SVWKTWTWRSEGDLMMNGAFFVTSG-----DQRKKRPFSKMDMISYREGTYVTRMTRMSG 438
             WK+W W+SEGD  +NGA+FV SG       + K P      I  + GT V ++T  +G
Sbjct: 390 GEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLTMDAG 449

BLAST of Cp4.1LG01g12640 vs. TAIR10
Match: AT2G02720.1 (AT2G02720.1 Pectate lyase family protein)

HSP 1 Score: 498.0 bits (1281), Expect = 5.7e-141
Identity = 252/455 (55.38%), Postives = 322/455 (70.77%), Query Frame = 1

Query: 1   MVAPTSRAMLGVLLTLSLVA-TAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDIL 60
           MV   S   + V L+L++V  +  A I  +D+YW  R   A +   +++DPNPENVTD  
Sbjct: 1   MVNLGSYVFVFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHF 60

Query: 61  N--AEVEQELSGS-NDTRRNLK---------RYKGPCLATNPIDRCWRCDPNWAKNRKKL 120
           N  A +  E +G  N+TRR+L+         R  G   + N ID+CWR D NW KNRKKL
Sbjct: 61  NYHAALAMETTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKL 120

Query: 121 ADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRL 180
           ADCVLGFG KT GGK GP YVV D SD+D+++PKPGT+RHAV +  PLWI F+ +M I+L
Sbjct: 121 ADCVLGFGRKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKL 180

Query: 181 TQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHV 240
            QELI+++DKTID RGA + I  GAG+TLQ+V+NVIIH + I  I  G+GG+I D+  H 
Sbjct: 181 QQELIITNDKTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHF 240

Query: 241 GLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFG 300
           GLRT+SDGDGI+IFG++N+WIDHVSM++C DG+IDAIMGSTAITISNSHFT H++VMLFG
Sbjct: 241 GLRTVSDGDGINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFG 300

Query: 301 ASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIII 360
            ++    D+ MQ+TVAFNHFG+ L QRMPR R+G  HVVNNDYTHW MYAIGG+ +P II
Sbjct: 301 GTNKDVIDKKMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTII 360

Query: 361 SQGNRFIAPPNVNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSG-----DQRKK 420
           SQGNRFIAPP  ++K+VTKREY+P   WK+W W+SE D  +NGA+FV SG         K
Sbjct: 361 SQGNRFIAPPIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPK 420

Query: 421 RPFSKMDMISYREGTYVTRMTRMSGALACFIGRPC 438
            P  +   I  + GT V R+T+ +G L C  G+ C
Sbjct: 421 NPIPRKFAIRPQPGTKVRRLTKDAGTLGCKPGKSC 455

BLAST of Cp4.1LG01g12640 vs. TAIR10
Match: AT4G13710.1 (AT4G13710.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 465.3 bits (1196), Expect = 4.1e-131
Identity = 225/387 (58.14%), Postives = 279/387 (72.09%), Query Frame = 1

Query: 51  NPENVTDILNAEVEQELSGSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADC 110
           +P+ V D + A++ Q    +   RR L  +   C   NPID CWRCD NW KNRK+LADC
Sbjct: 88  DPDMVADEV-AKLVQMSEQNRTARRKLGFFS--CGTGNPIDDCWRCDRNWHKNRKRLADC 147

Query: 111 VLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQE 170
            +GFG   IGG+ G FY+V D +D D++ PKPGTLRHAVIQ+ PLWI F  +M I L QE
Sbjct: 148 GIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQE 207

Query: 171 LIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLR 230
           LI++S KTIDARG+NV IA GA IT+Q++ NVIIHGL IH        M+R +  H G R
Sbjct: 208 LIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWR 267

Query: 231 TMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASD 290
           TM+DGD +SIFGSS+IWIDH S+S+C DGL+DA+MGSTAIT+SN+HFTHHN+VML G SD
Sbjct: 268 TMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSD 327

Query: 291 GYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQG 350
            Y+ D++MQVT+A+NHFG+GLVQRMPRCR G+FHVVNNDYTHW MYAIGGS  P I SQG
Sbjct: 328 SYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQG 387

Query: 351 NRFIAPPNVNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDM 410
           NR+ AP +  AKEVTKR  +  S WK W WRSEGDL++NGAFF  SG       + +   
Sbjct: 388 NRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSG-AGASASYGRASS 447

Query: 411 ISYREGTYVTRMTRMSGALACFIGRPC 438
           ++ +  + V  +T  +GAL C  GRPC
Sbjct: 448 LAAKPSSMVDTITSTAGALGCRKGRPC 470

BLAST of Cp4.1LG01g12640 vs. NCBI nr
Match: gi|659091001|ref|XP_008446316.1| (PREDICTED: probable pectate lyase P59 [Cucumis melo])

HSP 1 Score: 805.8 bits (2080), Expect = 3.6e-230
Identity = 383/431 (88.86%), Postives = 408/431 (94.66%), Query Frame = 1

Query: 8   AMLGVLLTLSLVAT-AYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQE 67
           A L +LLTLSL+AT A+ADIG+FDDYWK+RAA+AE+AAA A++PNPE + D LNAEV Q 
Sbjct: 2   ARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQA 61

Query: 68  LSGSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPF 127
           LSGSN TRRNLK+Y GPC ATNPIDRCWRCDPNWA+NRKKLADCVLGFG KTIGGKLGP+
Sbjct: 62  LSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGPY 121

Query: 128 YVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANV 187
           YVVNDSSDSD+M PKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIV+SDKTIDARGANV
Sbjct: 122 YVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGANV 181

Query: 188 QIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNI 247
           QIAYGAGITLQYV+NVIIHGLRIHHIV+G+GGMIRDAVDHVGLRTMSDGDGISIFGSSN+
Sbjct: 182 QIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSNV 241

Query: 248 WIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNH 307
           WIDHVSMSNCQDGLIDAIMGSTAITISN HFTHHN+VMLFGASDGYSNDQIMQVTVAFNH
Sbjct: 242 WIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFNH 301

Query: 308 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTK 367
           FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTK
Sbjct: 302 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTK 361

Query: 368 REYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMS 427
           REYSPE VWK+WTWRSEGDLMMNGAFFVTSGDQRK+RPFS  DMISY+ GTYV RMTR S
Sbjct: 362 REYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRFS 421

Query: 428 GALACFIGRPC 438
           GALACF+GRPC
Sbjct: 422 GALACFVGRPC 432

BLAST of Cp4.1LG01g12640 vs. NCBI nr
Match: gi|449435358|ref|XP_004135462.1| (PREDICTED: probable pectate lyase P59 [Cucumis sativus])

HSP 1 Score: 786.6 bits (2030), Expect = 2.3e-224
Identity = 370/429 (86.25%), Postives = 401/429 (93.47%), Query Frame = 1

Query: 10  LGVLLTLSLVAT-AYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELS 69
           L +LLTLSL+AT A+ADIG+FDDYWK+RAA AEKAAA A+DP+PE V + +NAE+    S
Sbjct: 4   LSLLLTLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYDPDPETVANSVNAEIAHASS 63

Query: 70  GSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYV 129
           G N TRRNLK+Y GPCLATNPIDRCWRCDPNWA+NRKKLADCVLGFG KT GGKLGP+YV
Sbjct: 64  GRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYV 123

Query: 130 VNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQI 189
           VNDSSDSD+M PKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI++SDKTIDARGANVQI
Sbjct: 124 VNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQI 183

Query: 190 AYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWI 249
           AYGAGITLQY++NVIIHGLRIHHIV+GSGGMIRDAVDHVGLRTMSDGDGISIFGSSN+WI
Sbjct: 184 AYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWI 243

Query: 250 DHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFG 309
           DHVSMSNC DGLIDAIMGSTAITISN HFTHHN+VMLFGASDGYS DQIMQ+TVAFNHFG
Sbjct: 244 DHVSMSNCHDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQITVAFNHFG 303

Query: 310 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKRE 369
           QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTKRE
Sbjct: 304 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTKRE 363

Query: 370 YSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGA 429
           YSPE VWK+WTWRSEGDLMMNGAFFVTSGDQ K+RPFS+MDMISY+ GTYV RMTR +G+
Sbjct: 364 YSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVKRMTRFAGS 423

Query: 430 LACFIGRPC 438
           LACF+GRPC
Sbjct: 424 LACFVGRPC 432

BLAST of Cp4.1LG01g12640 vs. NCBI nr
Match: gi|702421940|ref|XP_010066949.1| (PREDICTED: pectate lyase [Eucalyptus grandis])

HSP 1 Score: 676.4 bits (1744), Expect = 3.3e-191
Identity = 314/420 (74.76%), Postives = 361/420 (85.95%), Query Frame = 1

Query: 18  LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSNDTRRNL 77
           LV +  A+I  FD+YW  RA +A+K A KA+DP+PE VT + NA+V++ + G+N TRR L
Sbjct: 17  LVPSLQANIEEFDEYWAKRAEEAQKDALKAYDPHPETVTSLFNAKVDKAVRGNNSTRRQL 76

Query: 78  KRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDI 137
           K+YKGPCLATNPIDRCWRC+ NWA+NRK+LA+CVLGFG KT GGK G FYVV DSSD+D+
Sbjct: 77  KKYKGPCLATNPIDRCWRCNRNWARNRKRLANCVLGFGRKTRGGKRGWFYVVTDSSDNDM 136

Query: 138 MEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQ 197
           + PKPGTLRHAV+QKGPLWI F+ +M IRL QELI++SDKTIDARGANV IAYGAGITLQ
Sbjct: 137 VNPKPGTLRHAVVQKGPLWIIFAGSMVIRLNQELIMTSDKTIDARGANVHIAYGAGITLQ 196

Query: 198 YVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQ 257
           +++NVIIHGL IH IV GSGG+IRDA DHVGLRT SDGDGISIFGSS++WIDHVSMS C 
Sbjct: 197 FIRNVIIHGLHIHDIVRGSGGLIRDAHDHVGLRTASDGDGISIFGSSHLWIDHVSMSACS 256

Query: 258 DGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPR 317
           DGLIDAI GSTAITISN HFTHHN+VMLFGASDGYS DQIMQ+TVAFNHFG+GLVQRMPR
Sbjct: 257 DGLIDAIQGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQITVAFNHFGRGLVQRMPR 316

Query: 318 CRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWKT 377
           CRWGFFHVVNNDYTHWLMYAIGGSQHP IISQGNRFIAPPN+ AKEVTKR+Y+ E  WK 
Sbjct: 317 CRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNIAAKEVTKRDYATEDQWKN 376

Query: 378 WTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALACFIGRPC 437
           W W+SEGDLMMNGAFFV SG+ R + PFS+ D+I  + GTYV R+TR +G L C +GRPC
Sbjct: 377 WLWKSEGDLMMNGAFFVNSGNPRARFPFSRRDVIKAKPGTYVRRLTRFAGKLGCRVGRPC 436

BLAST of Cp4.1LG01g12640 vs. NCBI nr
Match: gi|659085461|ref|XP_008443432.1| (PREDICTED: probable pectate lyase 19 [Cucumis melo])

HSP 1 Score: 661.8 bits (1706), Expect = 8.5e-187
Identity = 310/441 (70.29%), Postives = 366/441 (82.99%), Query Frame = 1

Query: 1   MVAPTSRAMLGVLLTLS-LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDIL 60
           MVA     +L VL++++ L+ T  A I  FDDY   +A +A++A+ +AF P+P NVTD  
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NAEVE---QELSGSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGW 120
           N  V    + + GSN TRR+L +YKGPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFG 
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSD 180
           KT GGK G  YVV D SD+D++ PKPGTLR+  +QK PLWI F+ +M IRL++EL+V+S 
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGD 240
           KTIDARG+NV IAYGAG+++Q+ +NVIIHGLRIHH++   GGMIRD  +HVGLRT+SDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQ 300
           GISIFGSSNIW+DH+SMSNCQDGLIDAI GSTAITISNSHFTHHNDVMLFGASD Y  D 
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAP 360
           IMQVTVAFNHFG+GLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP IISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNVNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREG 420
           PN  A+++T R Y+PESVWKTWTWRSEGDLMMNGA+F+ SG+  K+RP+S+ DMI  + G
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TYVTRMTRMSGALACFIGRPC 438
           T+V R+TR SG+L CF+GRPC
Sbjct: 421 TFVRRLTRFSGSLNCFVGRPC 441

BLAST of Cp4.1LG01g12640 vs. NCBI nr
Match: gi|703158829|ref|XP_010112093.1| (putative pectate lyase 7 [Morus notabilis])

HSP 1 Score: 654.4 bits (1687), Expect = 1.4e-184
Identity = 304/437 (69.57%), Postives = 359/437 (82.15%), Query Frame = 1

Query: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MV  T   ++ ++  + L+ +  A+IG+FD+ W+ RA +A KAA +A+DPNPE VTD  N
Sbjct: 1   MVGTTKLGLVFLIGFVVLIPSLQANIGDFDESWQQRAEEAHKAALEAYDPNPEEVTDSFN 60

Query: 61  AEVEQELSGSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120
            EV++ + GSN TRR L+RY GPC ATNPIDRCWRC  NWA NRK+LA CVLGFG KT G
Sbjct: 61  EEVDKVIDGSNSTRRELRRYSGPCAATNPIDRCWRCRSNWASNRKRLARCVLGFGRKTTG 120

Query: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           G+ G +YVV D SD+D+  P+PGTLRHAVIQK PLWI F+ +M IRL+QEL+++S KTID
Sbjct: 121 GRSGRYYVVTDPSDNDVQNPRPGTLRHAVIQKQPLWIIFAHSMIIRLSQELLITSHKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISI 240
           ARGAN+ IA GAGIT+Q+ +NVIIHGL IH IV   GGMIRD+VDH G RT +DGDGISI
Sbjct: 181 ARGANIHIANGAGITIQFARNVIIHGLHIHDIVASGGGMIRDSVDHFGFRTPADGDGISI 240

Query: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGS+NIW+DH+SMSNCQDGLIDAI GSTAITISN HFTHHNDVMLFGASD +  D+IMQV
Sbjct: 241 FGSTNIWLDHISMSNCQDGLIDAIQGSTAITISNCHFTHHNDVMLFGASDSFEGDKIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFG+GLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGS HP IISQGNRFIAPPN  
Sbjct: 301 TVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSSHPTIISQGNRFIAPPNNA 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AK+VTKR+Y+ E  WK WTWRSEGDLMMNGAFFV SG++ KKRPF++ DMI  + GT+VT
Sbjct: 361 AKQVTKRDYAAEWEWKRWTWRSEGDLMMNGAFFVQSGNRSKKRPFTRHDMIKAKPGTFVT 420

Query: 421 RMTRMSGALACFIGRPC 438
           R+TR +GAL C+ G+ C
Sbjct: 421 RLTRFAGALNCYAGKKC 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PLY59_SOLLC3.3e-16263.66Probable pectate lyase P59 OS=Solanum lycopersicum GN=LAT59 PE=2 SV=1[more]
PLY_LILLO5.2e-15261.74Pectate lyase OS=Lilium longiflorum PE=2 SV=1[more]
PLY7_ARATH1.1e-14956.03Probable pectate lyase 7 OS=Arabidopsis thaliana GN=At3g01270 PE=2 SV=1[more]
PLY19_ARATH1.6e-14856.48Probable pectate lyase 19 OS=Arabidopsis thaliana GN=At5g15110 PE=2 SV=1[more]
PLY3_ARATH4.6e-14858.84Probable pectate lyase 3 OS=Arabidopsis thaliana GN=AT59 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KSL4_CUCSA1.6e-22486.25Pectate lyase OS=Cucumis sativus GN=Csa_5G604340 PE=3 SV=1[more]
A0A059BFL8_EUCGR2.3e-19174.76Pectate lyase OS=Eucalyptus grandis GN=EUGRSUZ_G02541 PE=3 SV=1[more]
W9SK59_9ROSA9.5e-18569.57Pectate lyase OS=Morus notabilis GN=L484_001952 PE=3 SV=1[more]
W9S2X9_9ROSA2.8e-18472.62Pectate lyase OS=Morus notabilis GN=L484_014236 PE=3 SV=1[more]
A0A0A0LCU3_CUCSA3.0e-18369.77Pectate lyase OS=Cucumis sativus GN=Csa_3G827350 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G01270.16.1e-15156.03 Pectate lyase family protein[more]
AT5G15110.11.2e-14956.26 Pectate lyase family protein[more]
AT1G14420.12.6e-14958.84 Pectate lyase family protein[more]
AT2G02720.15.7e-14155.38 Pectate lyase family protein[more]
AT4G13710.14.1e-13158.14 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659091001|ref|XP_008446316.1|3.6e-23088.86PREDICTED: probable pectate lyase P59 [Cucumis melo][more]
gi|449435358|ref|XP_004135462.1|2.3e-22486.25PREDICTED: probable pectate lyase P59 [Cucumis sativus][more]
gi|702421940|ref|XP_010066949.1|3.3e-19174.76PREDICTED: pectate lyase [Eucalyptus grandis][more]
gi|659085461|ref|XP_008443432.1|8.5e-18770.29PREDICTED: probable pectate lyase 19 [Cucumis melo][more]
gi|703158829|ref|XP_010112093.1|1.4e-18469.57putative pectate lyase 7 [Morus notabilis][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0030570pectate lyase activity
Vocabulary: INTERPRO
TermDefinition
IPR018082AmbAllergen
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR007524Pec_lyase_N
IPR002022Pec_lyase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g12640.1Cp4.1LG01g12640.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002022Pectate lyase/Amb allergenPFAMPF00544Pec_lyase_Ccoord: 172..353
score: 8.1
IPR002022Pectate lyase/Amb allergenSMARTSM00656amb_allcoord: 162..359
score: 9.3E
IPR007524Pectate lyase, N-terminalPFAMPF04431Pec_lyase_Ncoord: 26..77
score: 7.2
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 88..428
score: 5.03
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 87..426
score: 5.5E
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 336..355
score: 4.3E-78coord: 378..402
score: 4.3E-78coord: 234..255
score: 4.3E-78coord: 408..431
score: 4.3E-78coord: 134..159
score: 4.3E-78coord: 110..127
score: 4.3E-78coord: 170..186
score: 4.3E-78coord: 314..333
score: 4.3
NoneNo IPR availablePANTHERPTHR31683FAMILY NOT NAMEDcoord: 12..437
score:
NoneNo IPR availablePANTHERPTHR31683:SF7PECTATE LYASE 19-RELATEDcoord: 12..437
score:

The following gene(s) are paralogous to this gene:

None