Cp4.1LG01g08670 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG01g08670 vs. Swiss-Prot
Match: CLCC_ARATH (Chloride channel protein CLC-c OS=Arabidopsis thaliana GN=CLC-C PE=1 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 2.0e-289
Identity = 496/641 (77.38%), Postives = 572/641 (89.24%), Query Frame = 1

Query: 95  YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 154
           Y+ AF  + G N++LA +AA+LCA+IAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI
Sbjct: 137 YFQAFFAFAGCNLILATAAASLCAFIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 196

Query: 155 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 214
           FGSIF VA GFVVGKEGPMVHTGACIA+LLGQGGS+KYRLTWKWLR+FKNDRDRRDLITC
Sbjct: 197 FGSIFGVAAGFVVGKEGPMVHTGACIANLLGQGGSKKYRLTWKWLRFFKNDRDRRDLITC 256

Query: 215 GAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCG 274
           GAAAGVAAAFRAPVGGVLFALEEAASWWR+ALLWRTFFTTAVVAVVLR  +EFCR G+CG
Sbjct: 257 GAAAGVAAAFRAPVGGVLFALEEAASWWRNALLWRTFFTTAVVAVVLRSLIEFCRSGRCG 316

Query: 275 LFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGP 334
           LFG+GGLIMF++++    Y TPDL+AIV LGVIGGV GSLYNYLVDKVLRTYSIINE+GP
Sbjct: 317 LFGKGGLIMFDVNSGPVLYSTPDLLAIVFLGVIGGVLGSLYNYLVDKVLRTYSIINEKGP 376

Query: 335 GSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLED-ECPTVGRSGNYKNFQCPPGQYND 394
             K++LV+++S+L++C +FGLPWLSQC PCP  +E+ +CP+VGRS  YK+FQCPP  YND
Sbjct: 377 RFKIMLVMAVSILSSCCAFGLPWLSQCTPCPIGIEEGKCPSVGRSSIYKSFQCPPNHYND 436

Query: 395 LASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILA 454
           L+SL  NTNDD IRNLFTS ++ +F +S+L IFF ++Y LGIITYGIA+PSGLFIPVILA
Sbjct: 437 LSSLLLNTNDDAIRNLFTSRSENEFHISTLAIFFVAVYCLGIITYGIAIPSGLFIPVILA 496

Query: 455 GASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLM 514
           GASYGR+VGRL G V+ LDV L++LLGAASFLGGTMRMTVSLCVILLELTNNLLMLPL+M
Sbjct: 497 GASYGRLVGRLLGPVSQLDVGLFSLLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLVM 556

Query: 515 LVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLITFSGIEKV 574
           LVLLISK+VAD FN+GVYDQIV MKGLP+ME HAEP+MR LVA  V SG LI+FS +EKV
Sbjct: 557 LVLLISKTVADCFNRGVYDQIVTMKGLPYMEDHAEPYMRNLVAKDVVSGALISFSRVEKV 616

Query: 575 DNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKKTSIRSEILR 634
             I  ALKMT HNGFPVIDEPPF+++SELCG+ LRSHLLVLL+ KKF+K++T+  S+ILR
Sbjct: 617 GVIWQALKMTRHNGFPVIDEPPFTEASELCGIALRSHLLVLLQGKKFSKQRTTFGSQILR 676

Query: 635 SFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHA 694
           S KA DF K G GKG+K+EDLD++EEEMEM+VDLHPITNTSPYTV+ET+SLAKAAILF  
Sbjct: 677 SCKARDFGKAGLGKGLKIEDLDLSEEEMEMYVDLHPITNTSPYTVLETLSLAKAAILFRQ 736

Query: 695 LGLRHLLVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNP 735
           LGLRHL VVPKTPGRPPI GILTRHDFMPE++LGLYP+++P
Sbjct: 737 LGLRHLCVVPKTPGRPPIVGILTRHDFMPEHVLGLYPHIDP 777

BLAST of Cp4.1LG01g08670 vs. Swiss-Prot
Match: CLCG_ARATH (Putative chloride channel-like protein CLC-g OS=Arabidopsis thaliana GN=CBSCLC6 PE=3 SV=2)

HSP 1 Score: 854.7 bits (2207), Expect = 7.2e-247
Identity = 426/748 (56.95%), Postives = 559/748 (74.73%), Query Frame = 1

Query: 23  SQDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICPIESLDYEV---------WEKRF 82
           S   DS+++    PLL S     ++TSQ+AIVG+N+CPIESLDYE+         W  R 
Sbjct: 4   STTEDSVAV----PLLPSLRRATNSTSQVAIVGANVCPIESLDYEIAENDFFKQDWRGRS 63

Query: 83  SCVWFGSVFI-----FCAQLSDDLVW--------------------------YYLAFAVY 142
               F  VF+     FC  +   L+                           + + F V+
Sbjct: 64  KVEIFQYVFMKWLLCFCIGIIVSLIGFANNLAVENLAGVKFVVTSNMMIAGRFAMGFVVF 123

Query: 143 VGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFAVA 202
             +N++L   A+ + A++APAAAGSGIPEVKAYLNG+DA  I +  TL +KI G+I AV+
Sbjct: 124 SVTNLILTLFASVITAFVAPAAAGSGIPEVKAYLNGVDAPEIFSLRTLIIKIIGNISAVS 183

Query: 203 GGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAGVAA 262
              ++GK GPMVHTGAC+AS+LGQGGS++YRLTW+WLR+FKNDRDRRDL+TCGAAAG+AA
Sbjct: 184 ASLLIGKAGPMVHTGACVASILGQGGSKRYRLTWRWLRFFKNDRDRRDLVTCGAAAGIAA 243

Query: 263 AFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCGLFGEGGLI 322
           +FRAPVGGVLFALEE +SWWRSALLWR FF+TAVVA+VLR  ++ C  G+CGLFG+GGLI
Sbjct: 244 SFRAPVGGVLFALEEMSSWWRSALLWRIFFSTAVVAIVLRALIDVCLSGKCGLFGKGGLI 303

Query: 323 MFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPGSKVILVV 382
           MF++++EN++Y   D++ ++LLGV+GG+ GSLYN+L+DKVLR Y+ I E+G   K++L  
Sbjct: 304 MFDVYSENASYHLGDVLPVLLLGVVGGILGSLYNFLLDKVLRAYNYIYEKGVTWKILLAC 363

Query: 383 SISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDLASLFFNTN 442
           +IS+ T+C+ FGLP+L+ C PCP +  +ECPT+GRSGN+K +QCPPG YNDLASL FNTN
Sbjct: 364 AISIFTSCLLFGLPFLASCQPCPVDALEECPTIGRSGNFKKYQCPPGHYNDLASLIFNTN 423

Query: 443 DDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAGASYGRIVG 502
           DD I+NLF+  TD +F   S+ +FF + +FL I +YGI  P+GLF+PVI+ GASYGR VG
Sbjct: 424 DDAIKNLFSKNTDFEFHYFSVLVFFVTCFFLSIFSYGIVAPAGLFVPVIVTGASYGRFVG 483

Query: 503 RLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLISKSV 562
            L GS + L+  L+A+LGAASFLGGTMRMTVS CVILLELTNNLL+LP++M+VLLISK+V
Sbjct: 484 MLLGSNSNLNHGLFAVLGAASFLGGTMRMTVSTCVILLELTNNLLLLPMMMVVLLISKTV 543

Query: 563 ADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLITFSGIEKVDNIMHALKM 622
           AD FN  +Y+ I+K+KG P++ +HAEP+MRQL+ G V +GPL  F+GIEKV+ I+H LK 
Sbjct: 544 ADGFNANIYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQVFNGIEKVETIVHVLKT 603

Query: 623 TNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKKTSIRSEILRSFKAYDFAK 682
           TNHNGFPV+D PP + +  L GL+LR+H+L LLK++ F     +  S  L  FKA +FAK
Sbjct: 604 TNHNGFPVVDGPPLAAAPVLHGLILRAHILTLLKKRVFMPSPVACDSNTLSQFKAEEFAK 663

Query: 683 PGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHALGLRHLLVV 731
            GSG+  K+ED++++EEE+ M++DLHP +N SPYTVVETMSLAKA ILF  +G+RHLLV+
Sbjct: 664 KGSGRSDKIEDVELSEEELNMYLDLHPFSNASPYTVVETMSLAKALILFREVGIRHLLVI 723

BLAST of Cp4.1LG01g08670 vs. Swiss-Prot
Match: CLCA_ARATH (Chloride channel protein CLC-a OS=Arabidopsis thaliana GN=CLC-A PE=1 SV=2)

HSP 1 Score: 710.3 bits (1832), Expect = 2.2e-203
Identity = 379/757 (50.07%), Postives = 512/757 (67.64%), Query Frame = 1

Query: 24  QDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICPIESLDYEV---------WEKRFS 83
           +  D  + TL +PLL+    R  +++ LA+VG+ +  IESLDYE+         W  R  
Sbjct: 19  EGEDPENNTLNQPLLKRH--RTLSSTPLALVGAKVSHIESLDYEINENDLFKHDWRSRSK 78

Query: 84  CVWFGSVF----------IFCAQLSDDL--------------VWYYLA-------FAVYV 143
              F  +F          +F   ++  +              V YY+A         V+ 
Sbjct: 79  AQVFQYIFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVGYYIAQDRFWTGLMVFT 138

Query: 144 GSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFAVAG 203
           G+N+ L   A  L  Y AP AAG GIPE+KAYLNGID  ++   +T+ VKI GSI AVA 
Sbjct: 139 GANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGFTTMMVKIVGSIGAVAA 198

Query: 204 GFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAGVAAA 263
           G  +GKEGP+VH G+CIASLLGQGG   +R+ W+WLRYF NDRDRRDLITCG+A+GV AA
Sbjct: 199 GLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRDRRDLITCGSASGVCAA 258

Query: 264 FRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCGLFGEGGLIM 323
           FR+PVGGVLFALEE A+WWRSALLWRTFF+TAVV VVLR F+E C  G+CGLFG GGLIM
Sbjct: 259 FRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEICNSGKCGLFGSGGLIM 318

Query: 324 FNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPGSKVILVVS 383
           F++      Y   D+I + L+GV GG+ GSLYN+L+ KVLR Y++IN++G   KV+L + 
Sbjct: 319 FDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYNLINQKGKIHKVLLSLG 378

Query: 384 ISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDLASLFFNTND 443
           +SL T+   FGLP+L++C PC P++++ CPT GRSGN+K F CP G YNDL++L   TND
Sbjct: 379 VSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCPNGYYNDLSTLLLTTND 438

Query: 444 DVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAGASYGRIVGR 503
           D +RN+F+S T  +F + SL+IFF     LG+IT+GIA PSGLF+P+IL G++YGR++G 
Sbjct: 439 DAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILMGSAYGRMLGT 498

Query: 504 LFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLISKSVA 563
             GS   +D  LYA+LGAAS + G+MRMTVSLCVI LELTNNLL+LP+ M VLLI+K+V 
Sbjct: 499 AMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVG 558

Query: 564 DIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGV--ASGPLITFSGIEKVDNIMHALK 623
           D FN  +Y+ I+ +KGLPF+EA+ EP+MR L  G +  A  P++T +G+EKV NI+  L+
Sbjct: 559 DSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLR 618

Query: 624 MTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKF-TKKKTSIRSEILRSFKAYDF 683
            T HN FPV+D    +  +EL GL+LR+HL+ +LK++ F  +K+ +   E+   F   + 
Sbjct: 619 NTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNEKRRTEEWEVREKFTPVEL 678

Query: 684 AKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHALGLRHLL 736
           A+    +    +D+ I   EM+++VDLHP+TNT+PYTVV++MS+AKA +LF ++GLRHLL
Sbjct: 679 AE----REDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAKALVLFRSVGLRHLL 738

BLAST of Cp4.1LG01g08670 vs. Swiss-Prot
Match: CLCB_ARATH (Chloride channel protein CLC-b OS=Arabidopsis thaliana GN=CLC-B PE=1 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 2.4e-202
Identity = 362/649 (55.78%), Postives = 474/649 (73.04%), Query Frame = 1

Query: 95  YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 154
           Y     V VG+N+ L   A+ LC   AP AAG GIPE+KAYLNG+D  ++   +T+ VKI
Sbjct: 127 YVTGLMVLVGANLGLTLVASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATTMIVKI 186

Query: 155 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 214
            GSI AVA G  +GKEGP+VH G+CIASLLGQGG+  +R+ W+WLRYF NDRDRRDLITC
Sbjct: 187 VGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGTDNHRIKWRWLRYFNNDRDRRDLITC 246

Query: 215 GAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCG 274
           G+AAGV AAFR+PVGGVLFALEE A+WWRSALLWRTFF+TAVV VVLR F+E C  G+CG
Sbjct: 247 GSAAGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLREFIEICNSGKCG 306

Query: 275 LFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGP 334
           LFG+GGLIMF++     TY   D+I ++L+GVIGG+ GSLYN+L+ KVLR Y++INE+G 
Sbjct: 307 LFGKGGLIMFDVSHVTYTYHVTDIIPVMLIGVIGGILGSLYNHLLHKVLRLYNLINEKGK 366

Query: 335 GSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDL 394
             KV+L +++SL T+   +GLP+L++C PC P++++ CPT GRSGN+K F CP G YNDL
Sbjct: 367 IHKVLLSLTVSLFTSVCLYGLPFLAKCKPCDPSIDEICPTNGRSGNFKQFHCPKGYYNDL 426

Query: 395 ASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAG 454
           A+L   TNDD +RNLF+S T  +F + SL+IFF     LG+ T+GIA PSGLF+P+IL G
Sbjct: 427 ATLLLTTNDDAVRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIATPSGLFLPIILMG 486

Query: 455 ASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLML 514
           A+YGR++G   GS  ++D  LYA+LGAA+ + G+MRMTVSLCVI LELTNNLL+LP+ M+
Sbjct: 487 AAYGRMLGAAMGSYTSIDQGLYAVLGAAALMAGSMRMTVSLCVIFLELTNNLLLLPITMI 546

Query: 515 VLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAG--GVASGPLITFSGIEK 574
           VLLI+K+V D FN  +YD I+ +KGLPF+EA+ EP+MR L  G  G A  P++T  G+EK
Sbjct: 547 VLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPPVVTLQGVEK 606

Query: 575 VDNIMHALKMTNHNGFPVIDEPPF------SDSSELCGLVLRSHLLVLLKEKKF-TKKKT 634
           V NI+  LK T HN FPV+DE         + ++EL GL+LR+HL+ +LK++ F T+K+ 
Sbjct: 607 VSNIVDVLKNTTHNAFPVLDEAEVPQVGLATGATELHGLILRAHLVKVLKKRWFLTEKRR 666

Query: 635 SIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLA 694
           +   E+   F   + A+    +    +D+ I   EMEM+VDLHP+TNT+PYTV+E MS+A
Sbjct: 667 TEEWEVREKFPWDELAE----REDNFDDVAITSAEMEMYVDLHPLTNTTPYTVMENMSVA 726

Query: 695 KAAILFHALGLRHLLVVPK--TPGRPPIAGILTRHDFMPEYILGLYPYL 733
           KA +LF  +GLRHLL+VPK    G  P+ GILTR D     IL  +P L
Sbjct: 727 KALVLFRQVGLRHLLIVPKIQASGMCPVVGILTRQDLRAYNILQAFPLL 771

BLAST of Cp4.1LG01g08670 vs. Swiss-Prot
Match: CLCD_ARATH (Chloride channel protein CLC-d OS=Arabidopsis thaliana GN=CLC-D PE=1 SV=2)

HSP 1 Score: 577.8 bits (1488), Expect = 1.7e-163
Identity = 328/644 (50.93%), Postives = 433/644 (67.24%), Query Frame = 1

Query: 95  YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 154
           Y+  F VY+  N+VL FS+A +    APAAAGSGIPE+K YLNGID    L   TL  KI
Sbjct: 117 YFAGFIVYLLINLVLVFSSAYIITQFAPAAAGSGIPEIKGYLNGIDIPGTLLFRTLIGKI 176

Query: 155 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 214
           FGSI +V GG  +GKEGP+VHTGACIASLLGQGGS KY L  +W + FK+DRDRRDL+TC
Sbjct: 177 FGSIGSVGGGLALGKEGPLVHTGACIASLLGQGGSTKYHLNSRWPQLFKSDRDRRDLVTC 236

Query: 215 GAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCG 274
           G AAGVAAAFRAPVGGVLFALEE  SWWRS L+WR FFT+A+VAVV+R  M +C+ G CG
Sbjct: 237 GCAAGVAAAFRAPVGGVLFALEEVTSWWRSQLMWRVFFTSAIVAVVVRTAMGWCKSGICG 296

Query: 275 LFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLR-TYSIINERG 334
            FG GG I++++      Y   +L+ + ++GVIGG+ G+L+N L   +     + ++++G
Sbjct: 297 HFGGGGFIIWDVSDGQDDYYFKELLPMAVIGVIGGLLGALFNQLTLYMTSWRRNSLHKKG 356

Query: 335 PGSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLED---ECP-TVGRSGNYKNFQC-PP 394
              K+I    IS +T+ ISFGLP L +C PCP ++ D   ECP   G  GNY NF C   
Sbjct: 357 NRVKIIEACIISCITSAISFGLPLLRKCSPCPESVPDSGIECPRPPGMYGNYVNFFCKTD 416

Query: 395 GQYNDLASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFI 454
            +YNDLA++FFNT DD IRNLF++ T ++F   SL  F    Y L ++T+G AVP+G F+
Sbjct: 417 NEYNDLATIFFNTQDDAIRNLFSAKTMREFSAQSLLTFLAMFYTLAVVTFGTAVPAGQFV 476

Query: 455 PVILAGASYGRIVGRL---FGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 514
           P I+ G++YGR+VG     F     ++   YALLGAASFLGG+MRMTVSLCVI++E+TNN
Sbjct: 477 PGIMIGSTYGRLVGMFVVRFYKKLNIEEGTYALLGAASFLGGSMRMTVSLCVIMVEITNN 536

Query: 515 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVA-GGVASGPL 574
           L +LPL+MLVLLISK+V D FN+G+Y+   ++KG+P +E+  +  MRQ++A     S  +
Sbjct: 537 LKLLPLIMLVLLISKAVGDAFNEGLYEVQARLKGIPLLESRPKYHMRQMIAKEACQSQKV 596

Query: 575 ITFSGIEKVDNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKK 634
           I+   + +V ++   L    HNGFPVID    S  + + GLVLRSHLLVLL+ K   +  
Sbjct: 597 ISLPRVIRVADVASILGSNKHNGFPVIDHTR-SGETLVIGLVLRSHLLVLLQSKVDFQHS 656

Query: 635 -----TSIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVV 694
                 S R+ I  SF   +FAKP S KG+ +ED+ +  +++EM++DL P  N SPY V 
Sbjct: 657 PLPCDPSARN-IRHSFS--EFAKPVSSKGLCIEDIHLTSDDLEMYIDLAPFLNPSPYVVP 716

Query: 695 ETMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPE 724
           E MSL K   LF  LGLRHL VVP+ P R  + G++TR D + E
Sbjct: 717 EDMSLTKVYNLFRQLGLRHLFVVPR-PSR--VIGLITRKDLLIE 753

BLAST of Cp4.1LG01g08670 vs. TrEMBL
Match: A0A0A0L832_CUCSA (Chloride channel protein OS=Cucumis sativus GN=Csa_3G111220 PE=3 SV=1)

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 660/775 (85.16%), Postives = 692/775 (89.29%), Query Frame = 1

Query: 1   MDESSENAREIDGSERQVESLFSQDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICP 60
           +DE+ +N REID S+R V S F++DRDS+S+T GEPLLR+STARISTTSQLAIVGSNICP
Sbjct: 14  IDENHDNNREIDPSDRLVGSPFTEDRDSVSLTFGEPLLRTSTARISTTSQLAIVGSNICP 73

Query: 61  IESLDYEV---------WEKR-----FSCVWFGSVFIFCAQLSDDLVW------------ 120
           IESLDYE+         W  R     F  +     F  C  L   LV             
Sbjct: 74  IESLDYEIIENELFKQDWRSRKKKEIFQYIILKWAFCLCIGLITGLVGFFNNIAVENIAG 133

Query: 121 --------------YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGID 180
                         Y+ AF VYVGSN  LA +AA LCAYIAPAAAGSGIPEVKAYLNGID
Sbjct: 134 HKLRLTNNLMLKEKYFQAFVVYVGSNSALAIAAAILCAYIAPAAAGSGIPEVKAYLNGID 193

Query: 181 AYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLR 240
           AYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLR
Sbjct: 194 AYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLR 253

Query: 241 YFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVV 300
           YFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVV
Sbjct: 254 YFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVV 313

Query: 301 LRGFMEFCRGGQCGLFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVD 360
           LR FMEFCRGGQCGLFGEGGLIMF I+TENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVD
Sbjct: 314 LRSFMEFCRGGQCGLFGEGGLIMFKINTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVD 373

Query: 361 KVLRTYSIINERGPGSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGN 420
           KVLRTYSIINERGPG+K+ILVV++S+LTTC+SFGLPWLSQCLPCP +L+D+CPTVGRSGN
Sbjct: 374 KVLRTYSIINERGPGTKLILVVAVSILTTCVSFGLPWLSQCLPCPTDLDDQCPTVGRSGN 433

Query: 421 YKNFQCPPGQYNDLASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGI 480
           YKNFQCPPG YNDLASLFFNTNDD IRNLFTSA DK FQLSSLF+FF SIY LGIITYGI
Sbjct: 434 YKNFQCPPGHYNDLASLFFNTNDDAIRNLFTSANDKHFQLSSLFVFFVSIYCLGIITYGI 493

Query: 481 AVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILL 540
           AVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILL
Sbjct: 494 AVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILL 553

Query: 541 ELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVA 600
           ELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVA
Sbjct: 554 ELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVA 613

Query: 601 SGPLITFSGIEKVDNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKF 660
           SGPLITFSGIEKVDNI+HALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLL+EKKF
Sbjct: 614 SGPLITFSGIEKVDNIVHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLREKKF 673

Query: 661 TKKKTSIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVE 720
           TKKK S+RSEI R FKA+DFAKPGSGKGVKLEDLD NEEE+EMFVDLHPITNTSPYTVVE
Sbjct: 674 TKKKVSLRSEIWRGFKAHDFAKPGSGKGVKLEDLDFNEEELEMFVDLHPITNTSPYTVVE 733

Query: 721 TMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNPH 736
           +MSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPE+ILGLYP+LNPH
Sbjct: 734 SMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNPH 788

BLAST of Cp4.1LG01g08670 vs. TrEMBL
Match: I1Z8C9_CUCSA (Chloride channel protein OS=Cucumis sativus GN=ClCc PE=2 SV=1)

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 601/661 (90.92%), Postives = 622/661 (94.10%), Query Frame = 1

Query: 95  YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 154
           Y+ AF VYVGSN  LA +AA LCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI
Sbjct: 57  YFQAFVVYVGSNSALAIAAAILCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 116

Query: 155 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 214
           FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC
Sbjct: 117 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 176

Query: 215 GAAAGVAAAFRAPVGGVLFALEEAASW--------------------WRSALLWRTFFTT 274
           GAAAGVAAAFRAPVGGVLFALEEAASW                    WRSALLWRTFFTT
Sbjct: 177 GAAAGVAAAFRAPVGGVLFALEEAASWSLSLSLSPQHRNIIHVEIVRWRSALLWRTFFTT 236

Query: 275 AVVAVVLRGFMEFCRGGQCGLFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSL 334
           AVVAVVLR FMEFCRGGQCGLFGEGGLIMF I+TENSTYGTPDLIAIVLLGVIGGVFGSL
Sbjct: 237 AVVAVVLRSFMEFCRGGQCGLFGEGGLIMFKINTENSTYGTPDLIAIVLLGVIGGVFGSL 296

Query: 335 YNYLVDKVLRTYSIINERGPGSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPT 394
           YNYLVDKVLRTYSIINERGPG+K+ILVV++S+LTTC+SFGLPWLSQCLPCP +L+D+CPT
Sbjct: 297 YNYLVDKVLRTYSIINERGPGTKLILVVAVSILTTCVSFGLPWLSQCLPCPTDLDDQCPT 356

Query: 395 VGRSGNYKNFQCPPGQYNDLASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLG 454
           VGRSGNYKNFQCPPG YNDLASLFFNTNDD IRNLFTSA DK FQLSSLF+FF SIY LG
Sbjct: 357 VGRSGNYKNFQCPPGHYNDLASLFFNTNDDAIRNLFTSANDKHFQLSSLFVFFVSIYCLG 416

Query: 455 IITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVS 514
           IITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVS
Sbjct: 417 IITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVS 476

Query: 515 LCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQL 574
           LCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQL
Sbjct: 477 LCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQL 536

Query: 575 VAGGVASGPLITFSGIEKVDNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVL 634
           VAGGVASGPLITFSGIEKVDNI+HALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVL
Sbjct: 537 VAGGVASGPLITFSGIEKVDNIVHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVL 596

Query: 635 LKEKKFTKKKTSIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTS 694
           L+EKKFTKKK S+RSEI R FKA+DFAKPGSGKGVKLEDLD NEEE+EMFVDLHPITNTS
Sbjct: 597 LREKKFTKKKVSLRSEIWRGFKAHDFAKPGSGKGVKLEDLDFNEEELEMFVDLHPITNTS 656

Query: 695 PYTVVETMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNP 736
           PYTVVE+MSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPE+ILGLYP+LNP
Sbjct: 657 PYTVVESMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNP 716

BLAST of Cp4.1LG01g08670 vs. TrEMBL
Match: B9SPD9_RICCO (Chloride channel protein OS=Ricinus communis GN=RCOM_0497360 PE=3 SV=1)

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 555/756 (73.41%), Postives = 635/756 (83.99%), Query Frame = 1

Query: 12  DGSERQVESLF---SQDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICPIESLDYEV 71
           +G + ++E  F   + D++  SM   EPLL  S  R + TSQ+AIVG+NICPIESLDYE 
Sbjct: 6   EGHDIEIEGSFGGMTDDKEYNSMR--EPLLYQS--RKNNTSQIAIVGANICPIESLDYEY 65

Query: 72  WEKRFSCVWFGSVFI-----FCAQLSD------------------------DLVWYYLAF 131
               FSC  F  ++I     F   LS                          L+WYYLAF
Sbjct: 66  ----FSCFPFSFIYIIIHIPFVVALSFAFIFIRNENNLDYKILMSAYWFLLALLWYYLAF 125

Query: 132 AVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIF 191
           A Y G N++LA +AAALCA+IAPAAAGSGIPEVKAYLNG+DA SILAPSTLFVKIFGS+F
Sbjct: 126 AAYAGCNLILAIAAAALCAFIAPAAAGSGIPEVKAYLNGVDAPSILAPSTLFVKIFGSVF 185

Query: 192 AVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAG 251
            VAGGFVVGKEGPMVHTGACIASLLGQGGSRKY LTW+WLRYFKNDRDRRDL+TCGAAAG
Sbjct: 186 GVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYHLTWRWLRYFKNDRDRRDLVTCGAAAG 245

Query: 252 VAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCGLFGEG 311
           VAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLR FM FC+ G+CGLFGEG
Sbjct: 246 VAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRAFMGFCQNGRCGLFGEG 305

Query: 312 GLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPGSKVI 371
           GLIM+++++  + Y TPDL+A++ LGVIGG FGSLYN+LVDKVLRTYSIINE+GP  K++
Sbjct: 306 GLIMYDVNSAKTNYNTPDLLAVIFLGVIGGAFGSLYNFLVDKVLRTYSIINEKGPTFKIL 365

Query: 372 LVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDLASLFF 431
           LV+ ISLLT+C S+GLPWL+ C+PCP +L + CPT GRSGN+KNFQCP   YNDLASLFF
Sbjct: 366 LVIIISLLTSCCSYGLPWLAPCIPCPTDLAEVCPTKGRSGNFKNFQCPSNHYNDLASLFF 425

Query: 432 NTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAGASYGR 491
           NTNDD IRNLFTS ++K FQ+S+L +FF ++YFLGIITYGIA+PSGLFIPVILAGASYGR
Sbjct: 426 NTNDDAIRNLFTSGSEKSFQVSTLLVFFVAVYFLGIITYGIAIPSGLFIPVILAGASYGR 485

Query: 492 IVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLIS 551
           +VG+L G ++ LDVSL++LLGAASFLGGTMRMTVSLC+ILLELTN+LLMLPL+MLVLLIS
Sbjct: 486 LVGKLLGPISDLDVSLFSLLGAASFLGGTMRMTVSLCIILLELTNDLLMLPLMMLVLLIS 545

Query: 552 KSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLITFSGIEKVDNIMHA 611
           K+VAD FNKGVYDQIVKMKGLP+MEAHAEP+MRQLVA  V SGPL++FSG+EKV NI+HA
Sbjct: 546 KTVADSFNKGVYDQIVKMKGLPYMEAHAEPYMRQLVASDVVSGPLVSFSGVEKVGNILHA 605

Query: 612 LKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKKTSIRSEILRSFKAYD 671
           LK T HNGFPVIDEPPFSD+SELCGLVLRSHLLVLLK KKFTK +    S IL  FKA+D
Sbjct: 606 LKTTGHNGFPVIDEPPFSDASELCGLVLRSHLLVLLKGKKFTKLRIMSGSGILTIFKAHD 665

Query: 672 FAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHALGLRHL 731
           FAK GSGKG+KLEDLDI  EEM+M+VDLHPITNTSPYTVVE+MSLAKAA+LF  LGLRHL
Sbjct: 666 FAKAGSGKGIKLEDLDITAEEMDMYVDLHPITNTSPYTVVESMSLAKAAVLFRELGLRHL 725

Query: 732 LVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNPH 736
            VVPKTPGRPPI GILTRHDF+PE+ILGLYP++NPH
Sbjct: 726 CVVPKTPGRPPIVGILTRHDFVPEHILGLYPHINPH 753

BLAST of Cp4.1LG01g08670 vs. TrEMBL
Match: W9RLC9_9ROSA (Chloride channel protein OS=Morus notabilis GN=L484_010967 PE=3 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 3.0e-308
Identity = 551/762 (72.31%), Postives = 628/762 (82.41%), Query Frame = 1

Query: 12  DGSERQVESLFSQDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICPIESLDYEV--- 71
           D ++ +VE L   +R+     L EPLL+S   RI  TSQ+AIVG+N+CPIESLDYE+   
Sbjct: 3   DETDIEVERL---EREGSGACLKEPLLKS---RIKYTSQIAIVGANVCPIESLDYEIVEN 62

Query: 72  ------WEKRFSCVWFGSVFI----------------------------FCAQLSDDLVW 131
                 W  R     +  VF                             F   L+++L+ 
Sbjct: 63  DLFKQDWRSRKKVEIYQYVFFKWALALLIGLCTGLVGFFNNIAVENIAGFKLLLTNNLML 122

Query: 132 ---YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLF 191
              Y+LAF VYV  N+VLA +AA LCAYIAPAAAGSGIPEVKAYLNGIDA+SILAPSTLF
Sbjct: 123 KEKYFLAFIVYVSCNMVLAAAAAVLCAYIAPAAAGSGIPEVKAYLNGIDAHSILAPSTLF 182

Query: 192 VKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDL 251
           VKIFGSIF VA GFVVGKEGPMVHTG+CIASLLGQGGSRKYRLTWKWLR+FKNDRDRRDL
Sbjct: 183 VKIFGSIFGVAAGFVVGKEGPMVHTGSCIASLLGQGGSRKYRLTWKWLRFFKNDRDRRDL 242

Query: 252 ITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGG 311
           ITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFM  C+GG
Sbjct: 243 ITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMGICKGG 302

Query: 312 QCGLFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINE 371
           +CGLFGEGGLIMF++ +  + Y   DL+A++ LG+IGG+ GSLYN+ V+KVLR YSIINE
Sbjct: 303 KCGLFGEGGLIMFDVTSTKAAYNAADLLAVIFLGIIGGILGSLYNFFVNKVLRIYSIINE 362

Query: 372 RGPGSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQY 431
           RGP  KVILV++IS+LT+C S+GLPWLSQC PCPP LED+CPT+GR GNYKNFQCPP QY
Sbjct: 363 RGPLFKVILVLTISILTSCCSYGLPWLSQCTPCPPYLEDQCPTIGRCGNYKNFQCPPNQY 422

Query: 432 NDLASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVI 491
           NDLASLFFNTNDD IRNLF++ ++K+F LS+LF+FF +IYFLGIITYGIAVPSGLFIPVI
Sbjct: 423 NDLASLFFNTNDDAIRNLFSAGSEKEFHLSTLFVFFIAIYFLGIITYGIAVPSGLFIPVI 482

Query: 492 LAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPL 551
           LAGASYGR+VG L G  + LD  L+ALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPL
Sbjct: 483 LAGASYGRLVGTLVGYFSDLDSGLFALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPL 542

Query: 552 LMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLITFSGIE 611
           +MLVLLISK+VAD FNKGVYDQI+ +KGLP+MEAHAEP+MR L+A  V SGPL+TFSGIE
Sbjct: 543 MMLVLLISKTVADCFNKGVYDQILVLKGLPYMEAHAEPYMRNLIASDVVSGPLVTFSGIE 602

Query: 612 KVDNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKKTSIRSEI 671
           KV NI+ ALK+T HNGFPVIDEPP SD+ ELCGLVLRSHLLVLLK KKF++++    S+I
Sbjct: 603 KVGNILLALKITRHNGFPVIDEPPLSDAPELCGLVLRSHLLVLLKGKKFSEQRVMTGSQI 662

Query: 672 LRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILF 731
           +R  KA+DFAK GSGKG+KLEDLDI E EMEM+VDLHPITN SPYTVVETMSLAKAA+LF
Sbjct: 663 MRKIKAHDFAKAGSGKGIKLEDLDITEAEMEMYVDLHPITNASPYTVVETMSLAKAAVLF 722

Query: 732 HALGLRHLLVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLN 734
             LGLRHLLVVPKTPGRPPI GILTRHDFMPE++LGLYP++N
Sbjct: 723 RDLGLRHLLVVPKTPGRPPIVGILTRHDFMPEHVLGLYPHIN 758

BLAST of Cp4.1LG01g08670 vs. TrEMBL
Match: U5GH92_POPTR (Chloride channel protein OS=Populus trichocarpa GN=POPTR_0004s23680g PE=3 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 5.8e-304
Identity = 534/743 (71.87%), Postives = 612/743 (82.37%), Query Frame = 1

Query: 33  LGEPLLRSSTARISTTSQLAIVGSNICPIESLDYEV---------WEKRFSCVWFGSVFI 92
           L EP L  +  R + TSQ+AIVG+N CPIESLDYE+         W  R     F  V +
Sbjct: 2   LREPFLVRN--RKNNTSQIAIVGANTCPIESLDYEIAENELLKQDWRSRKKAEIFQYVVL 61

Query: 93  ----------------FCAQLSDDLV---------------WYYLAFAVYVGSNIVLAFS 152
                           F   L+ + +                YY AFA Y G N+VLA +
Sbjct: 62  KWTLALLIGLGTGLVGFFNNLAIENIAGFKLLVTNNLMLKEMYYQAFATYAGCNVVLAIA 121

Query: 153 AAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFAVAGGFVVGKEGP 212
           AAALCAY+APAAAGSGIPEVKAYLNG+DA SILAP+TLFVKIFGSIF VA GFVVGKEGP
Sbjct: 122 AAALCAYVAPAAAGSGIPEVKAYLNGVDAPSILAPATLFVKIFGSIFGVAAGFVVGKEGP 181

Query: 213 MVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAGVAAAFRAPVGGVL 272
           MVHTGACIAS LGQGGSRKY LTWKWLRYFKNDRDRRDLITCG+AAGVAAAFRAPVGGVL
Sbjct: 182 MVHTGACIASFLGQGGSRKYHLTWKWLRYFKNDRDRRDLITCGSAAGVAAAFRAPVGGVL 241

Query: 273 FALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCGLFGEGGLIMFNIHTENST 332
           FALEEAASWWRSALLWRTFFTTAVVAVVLR  +EFCR G+CGLFG+GGLIMF++++  +T
Sbjct: 242 FALEEAASWWRSALLWRTFFTTAVVAVVLRSLIEFCRTGKCGLFGQGGLIMFDVNSTKAT 301

Query: 333 YGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPGSKVILVVSISLLTTCIS 392
           Y TPDL+A++ LGVIGGVFGS YNY VDKVLRTYSIINERGP  K++LV+ ISLLT+C S
Sbjct: 302 YSTPDLLAVMFLGVIGGVFGSFYNYCVDKVLRTYSIINERGPSFKILLVIVISLLTSCCS 361

Query: 393 FGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDLASLFFNTNDDVIRNLFTS 452
           +GLPWLSQC+PCPP+L ++CPT  RSGN+KNFQCPP  YN+LASLFFNTNDD IR LFTS
Sbjct: 362 YGLPWLSQCIPCPPHLAEQCPTESRSGNFKNFQCPPNHYNNLASLFFNTNDDAIRILFTS 421

Query: 453 ATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLD 512
            ++K+F LS+L +FF +I+ LGI+TYGIAVPSGLFIPVILAGASYGR+VG L G ++ LD
Sbjct: 422 GSEKEFDLSTLLVFFVAIFCLGIVTYGIAVPSGLFIPVILAGASYGRLVGTLLGPLSNLD 481

Query: 513 VSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYD 572
           V L+ALLGAASFLGGTMRMTVSLCVILLELTN+LLMLPL+MLVLLISKSVADIFNKGVYD
Sbjct: 482 VGLFALLGAASFLGGTMRMTVSLCVILLELTNDLLMLPLMMLVLLISKSVADIFNKGVYD 541

Query: 573 QIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLITFSGIEKVDNIMHALKMTNHNGFPVID 632
           QI+K+KGLP+ME HAEP+MR L+A  V SGPL++FSG+EKV NI+H L++T HNGFPVID
Sbjct: 542 QIMKIKGLPYMETHAEPYMRHLIASDVVSGPLVSFSGVEKVGNILHVLRVTRHNGFPVID 601

Query: 633 EPPFSDSSELCGLVLRSHLLVLLKEKKFTKKKTSIRSEILRSFKAYDFAKPGSGKGVKLE 692
           EPP+SD+ ELCGLVLRSHLLVLLK KKFTK++    S+I+R FKA+DFAK GSGKGVKLE
Sbjct: 602 EPPYSDAPELCGLVLRSHLLVLLKGKKFTKQRVKTGSDIVRGFKAHDFAKAGSGKGVKLE 661

Query: 693 DLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHALGLRHLLVVPKTPGRPPIA 736
           DL+I EEEMEM++DLHPI NTSPYTVVE+MSLAKAA+LF  LGLRHL VV KTPG PPI 
Sbjct: 662 DLEITEEEMEMYIDLHPIANTSPYTVVESMSLAKAAVLFRELGLRHLCVVSKTPGMPPIV 721

BLAST of Cp4.1LG01g08670 vs. TAIR10
Match: AT5G49890.1 (AT5G49890.1 chloride channel C)

HSP 1 Score: 996.1 bits (2574), Expect = 1.1e-290
Identity = 496/641 (77.38%), Postives = 572/641 (89.24%), Query Frame = 1

Query: 95  YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 154
           Y+ AF  + G N++LA +AA+LCA+IAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI
Sbjct: 137 YFQAFFAFAGCNLILATAAASLCAFIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 196

Query: 155 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 214
           FGSIF VA GFVVGKEGPMVHTGACIA+LLGQGGS+KYRLTWKWLR+FKNDRDRRDLITC
Sbjct: 197 FGSIFGVAAGFVVGKEGPMVHTGACIANLLGQGGSKKYRLTWKWLRFFKNDRDRRDLITC 256

Query: 215 GAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCG 274
           GAAAGVAAAFRAPVGGVLFALEEAASWWR+ALLWRTFFTTAVVAVVLR  +EFCR G+CG
Sbjct: 257 GAAAGVAAAFRAPVGGVLFALEEAASWWRNALLWRTFFTTAVVAVVLRSLIEFCRSGRCG 316

Query: 275 LFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGP 334
           LFG+GGLIMF++++    Y TPDL+AIV LGVIGGV GSLYNYLVDKVLRTYSIINE+GP
Sbjct: 317 LFGKGGLIMFDVNSGPVLYSTPDLLAIVFLGVIGGVLGSLYNYLVDKVLRTYSIINEKGP 376

Query: 335 GSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLED-ECPTVGRSGNYKNFQCPPGQYND 394
             K++LV+++S+L++C +FGLPWLSQC PCP  +E+ +CP+VGRS  YK+FQCPP  YND
Sbjct: 377 RFKIMLVMAVSILSSCCAFGLPWLSQCTPCPIGIEEGKCPSVGRSSIYKSFQCPPNHYND 436

Query: 395 LASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILA 454
           L+SL  NTNDD IRNLFTS ++ +F +S+L IFF ++Y LGIITYGIA+PSGLFIPVILA
Sbjct: 437 LSSLLLNTNDDAIRNLFTSRSENEFHISTLAIFFVAVYCLGIITYGIAIPSGLFIPVILA 496

Query: 455 GASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLM 514
           GASYGR+VGRL G V+ LDV L++LLGAASFLGGTMRMTVSLCVILLELTNNLLMLPL+M
Sbjct: 497 GASYGRLVGRLLGPVSQLDVGLFSLLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLVM 556

Query: 515 LVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLITFSGIEKV 574
           LVLLISK+VAD FN+GVYDQIV MKGLP+ME HAEP+MR LVA  V SG LI+FS +EKV
Sbjct: 557 LVLLISKTVADCFNRGVYDQIVTMKGLPYMEDHAEPYMRNLVAKDVVSGALISFSRVEKV 616

Query: 575 DNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKKTSIRSEILR 634
             I  ALKMT HNGFPVIDEPPF+++SELCG+ LRSHLLVLL+ KKF+K++T+  S+ILR
Sbjct: 617 GVIWQALKMTRHNGFPVIDEPPFTEASELCGIALRSHLLVLLQGKKFSKQRTTFGSQILR 676

Query: 635 SFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHA 694
           S KA DF K G GKG+K+EDLD++EEEMEM+VDLHPITNTSPYTV+ET+SLAKAAILF  
Sbjct: 677 SCKARDFGKAGLGKGLKIEDLDLSEEEMEMYVDLHPITNTSPYTVLETLSLAKAAILFRQ 736

Query: 695 LGLRHLLVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNP 735
           LGLRHL VVPKTPGRPPI GILTRHDFMPE++LGLYP+++P
Sbjct: 737 LGLRHLCVVPKTPGRPPIVGILTRHDFMPEHVLGLYPHIDP 777

BLAST of Cp4.1LG01g08670 vs. TAIR10
Match: AT5G33280.1 (AT5G33280.1 Voltage-gated chloride channel family protein)

HSP 1 Score: 854.7 bits (2207), Expect = 4.1e-248
Identity = 426/748 (56.95%), Postives = 559/748 (74.73%), Query Frame = 1

Query: 23  SQDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICPIESLDYEV---------WEKRF 82
           S   DS+++    PLL S     ++TSQ+AIVG+N+CPIESLDYE+         W  R 
Sbjct: 4   STTEDSVAV----PLLPSLRRATNSTSQVAIVGANVCPIESLDYEIAENDFFKQDWRGRS 63

Query: 83  SCVWFGSVFI-----FCAQLSDDLVW--------------------------YYLAFAVY 142
               F  VF+     FC  +   L+                           + + F V+
Sbjct: 64  KVEIFQYVFMKWLLCFCIGIIVSLIGFANNLAVENLAGVKFVVTSNMMIAGRFAMGFVVF 123

Query: 143 VGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFAVA 202
             +N++L   A+ + A++APAAAGSGIPEVKAYLNG+DA  I +  TL +KI G+I AV+
Sbjct: 124 SVTNLILTLFASVITAFVAPAAAGSGIPEVKAYLNGVDAPEIFSLRTLIIKIIGNISAVS 183

Query: 203 GGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAGVAA 262
              ++GK GPMVHTGAC+AS+LGQGGS++YRLTW+WLR+FKNDRDRRDL+TCGAAAG+AA
Sbjct: 184 ASLLIGKAGPMVHTGACVASILGQGGSKRYRLTWRWLRFFKNDRDRRDLVTCGAAAGIAA 243

Query: 263 AFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCGLFGEGGLI 322
           +FRAPVGGVLFALEE +SWWRSALLWR FF+TAVVA+VLR  ++ C  G+CGLFG+GGLI
Sbjct: 244 SFRAPVGGVLFALEEMSSWWRSALLWRIFFSTAVVAIVLRALIDVCLSGKCGLFGKGGLI 303

Query: 323 MFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPGSKVILVV 382
           MF++++EN++Y   D++ ++LLGV+GG+ GSLYN+L+DKVLR Y+ I E+G   K++L  
Sbjct: 304 MFDVYSENASYHLGDVLPVLLLGVVGGILGSLYNFLLDKVLRAYNYIYEKGVTWKILLAC 363

Query: 383 SISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDLASLFFNTN 442
           +IS+ T+C+ FGLP+L+ C PCP +  +ECPT+GRSGN+K +QCPPG YNDLASL FNTN
Sbjct: 364 AISIFTSCLLFGLPFLASCQPCPVDALEECPTIGRSGNFKKYQCPPGHYNDLASLIFNTN 423

Query: 443 DDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAGASYGRIVG 502
           DD I+NLF+  TD +F   S+ +FF + +FL I +YGI  P+GLF+PVI+ GASYGR VG
Sbjct: 424 DDAIKNLFSKNTDFEFHYFSVLVFFVTCFFLSIFSYGIVAPAGLFVPVIVTGASYGRFVG 483

Query: 503 RLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLISKSV 562
            L GS + L+  L+A+LGAASFLGGTMRMTVS CVILLELTNNLL+LP++M+VLLISK+V
Sbjct: 484 MLLGSNSNLNHGLFAVLGAASFLGGTMRMTVSTCVILLELTNNLLLLPMMMVVLLISKTV 543

Query: 563 ADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLITFSGIEKVDNIMHALKM 622
           AD FN  +Y+ I+K+KG P++ +HAEP+MRQL+ G V +GPL  F+GIEKV+ I+H LK 
Sbjct: 544 ADGFNANIYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQVFNGIEKVETIVHVLKT 603

Query: 623 TNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKKTSIRSEILRSFKAYDFAK 682
           TNHNGFPV+D PP + +  L GL+LR+H+L LLK++ F     +  S  L  FKA +FAK
Sbjct: 604 TNHNGFPVVDGPPLAAAPVLHGLILRAHILTLLKKRVFMPSPVACDSNTLSQFKAEEFAK 663

Query: 683 PGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHALGLRHLLVV 731
            GSG+  K+ED++++EEE+ M++DLHP +N SPYTVVETMSLAKA ILF  +G+RHLLV+
Sbjct: 664 KGSGRSDKIEDVELSEEELNMYLDLHPFSNASPYTVVETMSLAKALILFREVGIRHLLVI 723

BLAST of Cp4.1LG01g08670 vs. TAIR10
Match: AT5G40890.1 (AT5G40890.1 chloride channel A)

HSP 1 Score: 710.3 bits (1832), Expect = 1.2e-204
Identity = 379/757 (50.07%), Postives = 512/757 (67.64%), Query Frame = 1

Query: 24  QDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICPIESLDYEV---------WEKRFS 83
           +  D  + TL +PLL+    R  +++ LA+VG+ +  IESLDYE+         W  R  
Sbjct: 19  EGEDPENNTLNQPLLKRH--RTLSSTPLALVGAKVSHIESLDYEINENDLFKHDWRSRSK 78

Query: 84  CVWFGSVF----------IFCAQLSDDL--------------VWYYLA-------FAVYV 143
              F  +F          +F   ++  +              V YY+A         V+ 
Sbjct: 79  AQVFQYIFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVGYYIAQDRFWTGLMVFT 138

Query: 144 GSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFAVAG 203
           G+N+ L   A  L  Y AP AAG GIPE+KAYLNGID  ++   +T+ VKI GSI AVA 
Sbjct: 139 GANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGFTTMMVKIVGSIGAVAA 198

Query: 204 GFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAGVAAA 263
           G  +GKEGP+VH G+CIASLLGQGG   +R+ W+WLRYF NDRDRRDLITCG+A+GV AA
Sbjct: 199 GLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRDRRDLITCGSASGVCAA 258

Query: 264 FRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCGLFGEGGLIM 323
           FR+PVGGVLFALEE A+WWRSALLWRTFF+TAVV VVLR F+E C  G+CGLFG GGLIM
Sbjct: 259 FRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEICNSGKCGLFGSGGLIM 318

Query: 324 FNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPGSKVILVVS 383
           F++      Y   D+I + L+GV GG+ GSLYN+L+ KVLR Y++IN++G   KV+L + 
Sbjct: 319 FDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYNLINQKGKIHKVLLSLG 378

Query: 384 ISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDLASLFFNTND 443
           +SL T+   FGLP+L++C PC P++++ CPT GRSGN+K F CP G YNDL++L   TND
Sbjct: 379 VSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCPNGYYNDLSTLLLTTND 438

Query: 444 DVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAGASYGRIVGR 503
           D +RN+F+S T  +F + SL+IFF     LG+IT+GIA PSGLF+P+IL G++YGR++G 
Sbjct: 439 DAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILMGSAYGRMLGT 498

Query: 504 LFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLISKSVA 563
             GS   +D  LYA+LGAAS + G+MRMTVSLCVI LELTNNLL+LP+ M VLLI+K+V 
Sbjct: 499 AMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVG 558

Query: 564 DIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGV--ASGPLITFSGIEKVDNIMHALK 623
           D FN  +Y+ I+ +KGLPF+EA+ EP+MR L  G +  A  P++T +G+EKV NI+  L+
Sbjct: 559 DSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLR 618

Query: 624 MTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKF-TKKKTSIRSEILRSFKAYDF 683
            T HN FPV+D    +  +EL GL+LR+HL+ +LK++ F  +K+ +   E+   F   + 
Sbjct: 619 NTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNEKRRTEEWEVREKFTPVEL 678

Query: 684 AKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHALGLRHLL 736
           A+    +    +D+ I   EM+++VDLHP+TNT+PYTVV++MS+AKA +LF ++GLRHLL
Sbjct: 679 AE----REDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAKALVLFRSVGLRHLL 738

BLAST of Cp4.1LG01g08670 vs. TAIR10
Match: AT3G27170.1 (AT3G27170.1 chloride channel B)

HSP 1 Score: 706.8 bits (1823), Expect = 1.4e-203
Identity = 362/649 (55.78%), Postives = 474/649 (73.04%), Query Frame = 1

Query: 95  YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 154
           Y     V VG+N+ L   A+ LC   AP AAG GIPE+KAYLNG+D  ++   +T+ VKI
Sbjct: 127 YVTGLMVLVGANLGLTLVASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATTMIVKI 186

Query: 155 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 214
            GSI AVA G  +GKEGP+VH G+CIASLLGQGG+  +R+ W+WLRYF NDRDRRDLITC
Sbjct: 187 VGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGTDNHRIKWRWLRYFNNDRDRRDLITC 246

Query: 215 GAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCG 274
           G+AAGV AAFR+PVGGVLFALEE A+WWRSALLWRTFF+TAVV VVLR F+E C  G+CG
Sbjct: 247 GSAAGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLREFIEICNSGKCG 306

Query: 275 LFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGP 334
           LFG+GGLIMF++     TY   D+I ++L+GVIGG+ GSLYN+L+ KVLR Y++INE+G 
Sbjct: 307 LFGKGGLIMFDVSHVTYTYHVTDIIPVMLIGVIGGILGSLYNHLLHKVLRLYNLINEKGK 366

Query: 335 GSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDL 394
             KV+L +++SL T+   +GLP+L++C PC P++++ CPT GRSGN+K F CP G YNDL
Sbjct: 367 IHKVLLSLTVSLFTSVCLYGLPFLAKCKPCDPSIDEICPTNGRSGNFKQFHCPKGYYNDL 426

Query: 395 ASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAG 454
           A+L   TNDD +RNLF+S T  +F + SL+IFF     LG+ T+GIA PSGLF+P+IL G
Sbjct: 427 ATLLLTTNDDAVRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIATPSGLFLPIILMG 486

Query: 455 ASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLML 514
           A+YGR++G   GS  ++D  LYA+LGAA+ + G+MRMTVSLCVI LELTNNLL+LP+ M+
Sbjct: 487 AAYGRMLGAAMGSYTSIDQGLYAVLGAAALMAGSMRMTVSLCVIFLELTNNLLLLPITMI 546

Query: 515 VLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAG--GVASGPLITFSGIEK 574
           VLLI+K+V D FN  +YD I+ +KGLPF+EA+ EP+MR L  G  G A  P++T  G+EK
Sbjct: 547 VLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPPVVTLQGVEK 606

Query: 575 VDNIMHALKMTNHNGFPVIDEPPF------SDSSELCGLVLRSHLLVLLKEKKF-TKKKT 634
           V NI+  LK T HN FPV+DE         + ++EL GL+LR+HL+ +LK++ F T+K+ 
Sbjct: 607 VSNIVDVLKNTTHNAFPVLDEAEVPQVGLATGATELHGLILRAHLVKVLKKRWFLTEKRR 666

Query: 635 SIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLA 694
           +   E+   F   + A+    +    +D+ I   EMEM+VDLHP+TNT+PYTV+E MS+A
Sbjct: 667 TEEWEVREKFPWDELAE----REDNFDDVAITSAEMEMYVDLHPLTNTTPYTVMENMSVA 726

Query: 695 KAAILFHALGLRHLLVVPK--TPGRPPIAGILTRHDFMPEYILGLYPYL 733
           KA +LF  +GLRHLL+VPK    G  P+ GILTR D     IL  +P L
Sbjct: 727 KALVLFRQVGLRHLLIVPKIQASGMCPVVGILTRQDLRAYNILQAFPLL 771

BLAST of Cp4.1LG01g08670 vs. TAIR10
Match: AT5G26240.1 (AT5G26240.1 chloride channel D)

HSP 1 Score: 577.8 bits (1488), Expect = 9.6e-165
Identity = 328/644 (50.93%), Postives = 433/644 (67.24%), Query Frame = 1

Query: 95  YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 154
           Y+  F VY+  N+VL FS+A +    APAAAGSGIPE+K YLNGID    L   TL  KI
Sbjct: 117 YFAGFIVYLLINLVLVFSSAYIITQFAPAAAGSGIPEIKGYLNGIDIPGTLLFRTLIGKI 176

Query: 155 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 214
           FGSI +V GG  +GKEGP+VHTGACIASLLGQGGS KY L  +W + FK+DRDRRDL+TC
Sbjct: 177 FGSIGSVGGGLALGKEGPLVHTGACIASLLGQGGSTKYHLNSRWPQLFKSDRDRRDLVTC 236

Query: 215 GAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCG 274
           G AAGVAAAFRAPVGGVLFALEE  SWWRS L+WR FFT+A+VAVV+R  M +C+ G CG
Sbjct: 237 GCAAGVAAAFRAPVGGVLFALEEVTSWWRSQLMWRVFFTSAIVAVVVRTAMGWCKSGICG 296

Query: 275 LFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLR-TYSIINERG 334
            FG GG I++++      Y   +L+ + ++GVIGG+ G+L+N L   +     + ++++G
Sbjct: 297 HFGGGGFIIWDVSDGQDDYYFKELLPMAVIGVIGGLLGALFNQLTLYMTSWRRNSLHKKG 356

Query: 335 PGSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLED---ECP-TVGRSGNYKNFQC-PP 394
              K+I    IS +T+ ISFGLP L +C PCP ++ D   ECP   G  GNY NF C   
Sbjct: 357 NRVKIIEACIISCITSAISFGLPLLRKCSPCPESVPDSGIECPRPPGMYGNYVNFFCKTD 416

Query: 395 GQYNDLASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFI 454
            +YNDLA++FFNT DD IRNLF++ T ++F   SL  F    Y L ++T+G AVP+G F+
Sbjct: 417 NEYNDLATIFFNTQDDAIRNLFSAKTMREFSAQSLLTFLAMFYTLAVVTFGTAVPAGQFV 476

Query: 455 PVILAGASYGRIVGRL---FGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNN 514
           P I+ G++YGR+VG     F     ++   YALLGAASFLGG+MRMTVSLCVI++E+TNN
Sbjct: 477 PGIMIGSTYGRLVGMFVVRFYKKLNIEEGTYALLGAASFLGGSMRMTVSLCVIMVEITNN 536

Query: 515 LLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVA-GGVASGPL 574
           L +LPL+MLVLLISK+V D FN+G+Y+   ++KG+P +E+  +  MRQ++A     S  +
Sbjct: 537 LKLLPLIMLVLLISKAVGDAFNEGLYEVQARLKGIPLLESRPKYHMRQMIAKEACQSQKV 596

Query: 575 ITFSGIEKVDNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKK 634
           I+   + +V ++   L    HNGFPVID    S  + + GLVLRSHLLVLL+ K   +  
Sbjct: 597 ISLPRVIRVADVASILGSNKHNGFPVIDHTR-SGETLVIGLVLRSHLLVLLQSKVDFQHS 656

Query: 635 -----TSIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVV 694
                 S R+ I  SF   +FAKP S KG+ +ED+ +  +++EM++DL P  N SPY V 
Sbjct: 657 PLPCDPSARN-IRHSFS--EFAKPVSSKGLCIEDIHLTSDDLEMYIDLAPFLNPSPYVVP 716

Query: 695 ETMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPE 724
           E MSL K   LF  LGLRHL VVP+ P R  + G++TR D + E
Sbjct: 717 EDMSLTKVYNLFRQLGLRHLFVVPR-PSR--VIGLITRKDLLIE 753

BLAST of Cp4.1LG01g08670 vs. NCBI nr
Match: gi|778676412|ref|XP_011650578.1| (PREDICTED: chloride channel protein CLC-c isoform X2 [Cucumis sativus])

HSP 1 Score: 1269.2 bits (3283), Expect = 0.0e+00
Identity = 652/735 (88.71%), Postives = 682/735 (92.79%), Query Frame = 1

Query: 1   MDESSENAREIDGSERQVESLFSQDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICP 60
           +DE+ +N REID S+R V S F++DRDS+S+T GEPLLR+STARISTTSQLAIVGSNICP
Sbjct: 14  IDENHDNNREIDPSDRLVGSPFTEDRDSVSLTFGEPLLRTSTARISTTSQLAIVGSNICP 73

Query: 61  IESLDYEVWEKRFSCVWFGSVFIFCAQLSDDLVWYYLAFAVYVGSNIVLAFSAAALCAYI 120
           IESLDYE                           Y+ AF VYVGSN  LA +AA LCAYI
Sbjct: 74  IESLDYE---------------------------YFQAFVVYVGSNSALAIAAAILCAYI 133

Query: 121 APAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACI 180
           APAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACI
Sbjct: 134 APAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACI 193

Query: 181 ASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAAS 240
           ASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAAS
Sbjct: 194 ASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAAS 253

Query: 241 WWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCGLFGEGGLIMFNIHTENSTYGTPDLIA 300
           WWRSALLWRTFFTTAVVAVVLR FMEFCRGGQCGLFGEGGLIMF I+TENSTYGTPDLIA
Sbjct: 254 WWRSALLWRTFFTTAVVAVVLRSFMEFCRGGQCGLFGEGGLIMFKINTENSTYGTPDLIA 313

Query: 301 IVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPGSKVILVVSISLLTTCISFGLPWLSQ 360
           IVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPG+K+ILVV++S+LTTC+SFGLPWLSQ
Sbjct: 314 IVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPGTKLILVVAVSILTTCVSFGLPWLSQ 373

Query: 361 CLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDLASLFFNTNDDVIRNLFTSATDKQFQL 420
           CLPCP +L+D+CPTVGRSGNYKNFQCPPG YNDLASLFFNTNDD IRNLFTSA DK FQL
Sbjct: 374 CLPCPTDLDDQCPTVGRSGNYKNFQCPPGHYNDLASLFFNTNDDAIRNLFTSANDKHFQL 433

Query: 421 SSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLG 480
           SSLF+FF SIY LGIITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLG
Sbjct: 434 SSLFVFFVSIYCLGIITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLG 493

Query: 481 AASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGL 540
           AASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGL
Sbjct: 494 AASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGL 553

Query: 541 PFMEAHAEPFMRQLVAGGVASGPLITFSGIEKVDNIMHALKMTNHNGFPVIDEPPFSDSS 600
           PFMEAHAEPFMRQLVAGGVASGPLITFSGIEKVDNI+HALKMTNHNGFPVIDEPPFSDSS
Sbjct: 554 PFMEAHAEPFMRQLVAGGVASGPLITFSGIEKVDNIVHALKMTNHNGFPVIDEPPFSDSS 613

Query: 601 ELCGLVLRSHLLVLLKEKKFTKKKTSIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEE 660
           ELCGLVLRSHLLVLL+EKKFTKKK S+RSEI R FKA+DFAKPGSGKGVKLEDLD NEEE
Sbjct: 614 ELCGLVLRSHLLVLLREKKFTKKKVSLRSEIWRGFKAHDFAKPGSGKGVKLEDLDFNEEE 673

Query: 661 MEMFVDLHPITNTSPYTVVETMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDF 720
           +EMFVDLHPITNTSPYTVVE+MSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDF
Sbjct: 674 LEMFVDLHPITNTSPYTVVESMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDF 721

Query: 721 MPEYILGLYPYLNPH 736
           MPE+ILGLYP+LNPH
Sbjct: 734 MPEHILGLYPHLNPH 721

BLAST of Cp4.1LG01g08670 vs. NCBI nr
Match: gi|449431884|ref|XP_004133730.1| (PREDICTED: chloride channel protein CLC-c isoform X1 [Cucumis sativus])

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 660/775 (85.16%), Postives = 692/775 (89.29%), Query Frame = 1

Query: 1   MDESSENAREIDGSERQVESLFSQDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICP 60
           +DE+ +N REID S+R V S F++DRDS+S+T GEPLLR+STARISTTSQLAIVGSNICP
Sbjct: 14  IDENHDNNREIDPSDRLVGSPFTEDRDSVSLTFGEPLLRTSTARISTTSQLAIVGSNICP 73

Query: 61  IESLDYEV---------WEKR-----FSCVWFGSVFIFCAQLSDDLVW------------ 120
           IESLDYE+         W  R     F  +     F  C  L   LV             
Sbjct: 74  IESLDYEIIENELFKQDWRSRKKKEIFQYIILKWAFCLCIGLITGLVGFFNNIAVENIAG 133

Query: 121 --------------YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGID 180
                         Y+ AF VYVGSN  LA +AA LCAYIAPAAAGSGIPEVKAYLNGID
Sbjct: 134 HKLRLTNNLMLKEKYFQAFVVYVGSNSALAIAAAILCAYIAPAAAGSGIPEVKAYLNGID 193

Query: 181 AYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLR 240
           AYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLR
Sbjct: 194 AYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLR 253

Query: 241 YFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVV 300
           YFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVV
Sbjct: 254 YFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVV 313

Query: 301 LRGFMEFCRGGQCGLFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVD 360
           LR FMEFCRGGQCGLFGEGGLIMF I+TENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVD
Sbjct: 314 LRSFMEFCRGGQCGLFGEGGLIMFKINTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVD 373

Query: 361 KVLRTYSIINERGPGSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGN 420
           KVLRTYSIINERGPG+K+ILVV++S+LTTC+SFGLPWLSQCLPCP +L+D+CPTVGRSGN
Sbjct: 374 KVLRTYSIINERGPGTKLILVVAVSILTTCVSFGLPWLSQCLPCPTDLDDQCPTVGRSGN 433

Query: 421 YKNFQCPPGQYNDLASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGI 480
           YKNFQCPPG YNDLASLFFNTNDD IRNLFTSA DK FQLSSLF+FF SIY LGIITYGI
Sbjct: 434 YKNFQCPPGHYNDLASLFFNTNDDAIRNLFTSANDKHFQLSSLFVFFVSIYCLGIITYGI 493

Query: 481 AVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILL 540
           AVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILL
Sbjct: 494 AVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILL 553

Query: 541 ELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVA 600
           ELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVA
Sbjct: 554 ELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVA 613

Query: 601 SGPLITFSGIEKVDNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKF 660
           SGPLITFSGIEKVDNI+HALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLL+EKKF
Sbjct: 614 SGPLITFSGIEKVDNIVHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLREKKF 673

Query: 661 TKKKTSIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVE 720
           TKKK S+RSEI R FKA+DFAKPGSGKGVKLEDLD NEEE+EMFVDLHPITNTSPYTVVE
Sbjct: 674 TKKKVSLRSEIWRGFKAHDFAKPGSGKGVKLEDLDFNEEELEMFVDLHPITNTSPYTVVE 733

Query: 721 TMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNPH 736
           +MSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPE+ILGLYP+LNPH
Sbjct: 734 SMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNPH 788

BLAST of Cp4.1LG01g08670 vs. NCBI nr
Match: gi|659102618|ref|XP_008452224.1| (PREDICTED: LOW QUALITY PROTEIN: chloride channel protein CLC-c [Cucumis melo])

HSP 1 Score: 1265.4 bits (3273), Expect = 0.0e+00
Identity = 659/775 (85.03%), Postives = 693/775 (89.42%), Query Frame = 1

Query: 1   MDESSENAREIDGSERQVESLFSQDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICP 60
           +DE+ +N REID S+R V S F++DRDS+S+TLGEPLLR+STARISTTSQLAIVGSNICP
Sbjct: 14  IDENHDNNREIDPSDRLVGSPFAEDRDSVSLTLGEPLLRTSTARISTTSQLAIVGSNICP 73

Query: 61  IESLDYEV---------WEKRFSCVWFGSVFI-----FCAQLSDDLVW------------ 120
           IESLDYE+         W  R     F  + +      C  L   LV             
Sbjct: 74  IESLDYEIIENELFKQDWRSRRKIEIFQYIILKWALCLCIGLITGLVGFFNNIAVENIAG 133

Query: 121 --------------YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGID 180
                         Y+ AF VYVGSN VLA +AA LCAYIAPAAAGSGIPEVKAYLNGID
Sbjct: 134 HKLLLTNNLMLKEKYFQAFVVYVGSNSVLAVAAAILCAYIAPAAAGSGIPEVKAYLNGID 193

Query: 181 AYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLR 240
           AYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLR
Sbjct: 194 AYSILAPSTLFVKIFGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLR 253

Query: 241 YFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVV 300
           YFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVV
Sbjct: 254 YFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVV 313

Query: 301 LRGFMEFCRGGQCGLFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVD 360
           LR FMEFCRGGQCGLFGEGGLIMF I+TENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVD
Sbjct: 314 LRSFMEFCRGGQCGLFGEGGLIMFKINTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVD 373

Query: 361 KVLRTYSIINERGPGSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGN 420
           KVLRTYSIINERGPG+K+ILVV++S+LTTC+SFGLPWLSQCLPCP +L+D+CPTVGRSGN
Sbjct: 374 KVLRTYSIINERGPGTKLILVVAVSILTTCVSFGLPWLSQCLPCPTDLDDQCPTVGRSGN 433

Query: 421 YKNFQCPPGQYNDLASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGI 480
           YKNFQCPPG YNDLASLFFNTNDD IRNLFTSA DK FQLSSLF+FF SIY LGIITYGI
Sbjct: 434 YKNFQCPPGHYNDLASLFFNTNDDAIRNLFTSANDKHFQLSSLFVFFVSIYCLGIITYGI 493

Query: 481 AVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILL 540
           AVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILL
Sbjct: 494 AVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILL 553

Query: 541 ELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVA 600
           ELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVA
Sbjct: 554 ELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVA 613

Query: 601 SGPLITFSGIEKVDNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKF 660
           SGPLITFSGIEKVDNI+HALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLL+EK F
Sbjct: 614 SGPLITFSGIEKVDNIVHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLREKXF 673

Query: 661 TKKKTSIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVE 720
           TKKK S+RSEI R FKA+DFAKPGSGKGVKLEDL+ NEEE+EMFVDLHPITNTSPYTVVE
Sbjct: 674 TKKKVSLRSEIWRGFKAHDFAKPGSGKGVKLEDLEFNEEELEMFVDLHPITNTSPYTVVE 733

Query: 721 TMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNPH 736
           +MSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPE+ILGLYP+LNPH
Sbjct: 734 SMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNPH 788

BLAST of Cp4.1LG01g08670 vs. NCBI nr
Match: gi|386649467|gb|AFJ15539.1| (chloride channel c [Cucumis sativus])

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 601/661 (90.92%), Postives = 622/661 (94.10%), Query Frame = 1

Query: 95  YYLAFAVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 154
           Y+ AF VYVGSN  LA +AA LCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI
Sbjct: 57  YFQAFVVYVGSNSALAIAAAILCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKI 116

Query: 155 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 214
           FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC
Sbjct: 117 FGSIFAVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITC 176

Query: 215 GAAAGVAAAFRAPVGGVLFALEEAASW--------------------WRSALLWRTFFTT 274
           GAAAGVAAAFRAPVGGVLFALEEAASW                    WRSALLWRTFFTT
Sbjct: 177 GAAAGVAAAFRAPVGGVLFALEEAASWSLSLSLSPQHRNIIHVEIVRWRSALLWRTFFTT 236

Query: 275 AVVAVVLRGFMEFCRGGQCGLFGEGGLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSL 334
           AVVAVVLR FMEFCRGGQCGLFGEGGLIMF I+TENSTYGTPDLIAIVLLGVIGGVFGSL
Sbjct: 237 AVVAVVLRSFMEFCRGGQCGLFGEGGLIMFKINTENSTYGTPDLIAIVLLGVIGGVFGSL 296

Query: 335 YNYLVDKVLRTYSIINERGPGSKVILVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPT 394
           YNYLVDKVLRTYSIINERGPG+K+ILVV++S+LTTC+SFGLPWLSQCLPCP +L+D+CPT
Sbjct: 297 YNYLVDKVLRTYSIINERGPGTKLILVVAVSILTTCVSFGLPWLSQCLPCPTDLDDQCPT 356

Query: 395 VGRSGNYKNFQCPPGQYNDLASLFFNTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLG 454
           VGRSGNYKNFQCPPG YNDLASLFFNTNDD IRNLFTSA DK FQLSSLF+FF SIY LG
Sbjct: 357 VGRSGNYKNFQCPPGHYNDLASLFFNTNDDAIRNLFTSANDKHFQLSSLFVFFVSIYCLG 416

Query: 455 IITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVS 514
           IITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVS
Sbjct: 417 IITYGIAVPSGLFIPVILAGASYGRIVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVS 476

Query: 515 LCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQL 574
           LCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQL
Sbjct: 477 LCVILLELTNNLLMLPLLMLVLLISKSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQL 536

Query: 575 VAGGVASGPLITFSGIEKVDNIMHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVL 634
           VAGGVASGPLITFSGIEKVDNI+HALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVL
Sbjct: 537 VAGGVASGPLITFSGIEKVDNIVHALKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVL 596

Query: 635 LKEKKFTKKKTSIRSEILRSFKAYDFAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTS 694
           L+EKKFTKKK S+RSEI R FKA+DFAKPGSGKGVKLEDLD NEEE+EMFVDLHPITNTS
Sbjct: 597 LREKKFTKKKVSLRSEIWRGFKAHDFAKPGSGKGVKLEDLDFNEEELEMFVDLHPITNTS 656

Query: 695 PYTVVETMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNP 736
           PYTVVE+MSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPE+ILGLYP+LNP
Sbjct: 657 PYTVVESMSLAKAAILFHALGLRHLLVVPKTPGRPPIAGILTRHDFMPEHILGLYPHLNP 716

BLAST of Cp4.1LG01g08670 vs. NCBI nr
Match: gi|223532782|gb|EEF34561.1| (chloride channel clc, putative [Ricinus communis])

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 555/756 (73.41%), Postives = 635/756 (83.99%), Query Frame = 1

Query: 12  DGSERQVESLF---SQDRDSISMTLGEPLLRSSTARISTTSQLAIVGSNICPIESLDYEV 71
           +G + ++E  F   + D++  SM   EPLL  S  R + TSQ+AIVG+NICPIESLDYE 
Sbjct: 6   EGHDIEIEGSFGGMTDDKEYNSMR--EPLLYQS--RKNNTSQIAIVGANICPIESLDYEY 65

Query: 72  WEKRFSCVWFGSVFI-----FCAQLSD------------------------DLVWYYLAF 131
               FSC  F  ++I     F   LS                          L+WYYLAF
Sbjct: 66  ----FSCFPFSFIYIIIHIPFVVALSFAFIFIRNENNLDYKILMSAYWFLLALLWYYLAF 125

Query: 132 AVYVGSNIVLAFSAAALCAYIAPAAAGSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIF 191
           A Y G N++LA +AAALCA+IAPAAAGSGIPEVKAYLNG+DA SILAPSTLFVKIFGS+F
Sbjct: 126 AAYAGCNLILAIAAAALCAFIAPAAAGSGIPEVKAYLNGVDAPSILAPSTLFVKIFGSVF 185

Query: 192 AVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYRLTWKWLRYFKNDRDRRDLITCGAAAG 251
            VAGGFVVGKEGPMVHTGACIASLLGQGGSRKY LTW+WLRYFKNDRDRRDL+TCGAAAG
Sbjct: 186 GVAGGFVVGKEGPMVHTGACIASLLGQGGSRKYHLTWRWLRYFKNDRDRRDLVTCGAAAG 245

Query: 252 VAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRGFMEFCRGGQCGLFGEG 311
           VAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLR FM FC+ G+CGLFGEG
Sbjct: 246 VAAAFRAPVGGVLFALEEAASWWRSALLWRTFFTTAVVAVVLRAFMGFCQNGRCGLFGEG 305

Query: 312 GLIMFNIHTENSTYGTPDLIAIVLLGVIGGVFGSLYNYLVDKVLRTYSIINERGPGSKVI 371
           GLIM+++++  + Y TPDL+A++ LGVIGG FGSLYN+LVDKVLRTYSIINE+GP  K++
Sbjct: 306 GLIMYDVNSAKTNYNTPDLLAVIFLGVIGGAFGSLYNFLVDKVLRTYSIINEKGPTFKIL 365

Query: 372 LVVSISLLTTCISFGLPWLSQCLPCPPNLEDECPTVGRSGNYKNFQCPPGQYNDLASLFF 431
           LV+ ISLLT+C S+GLPWL+ C+PCP +L + CPT GRSGN+KNFQCP   YNDLASLFF
Sbjct: 366 LVIIISLLTSCCSYGLPWLAPCIPCPTDLAEVCPTKGRSGNFKNFQCPSNHYNDLASLFF 425

Query: 432 NTNDDVIRNLFTSATDKQFQLSSLFIFFTSIYFLGIITYGIAVPSGLFIPVILAGASYGR 491
           NTNDD IRNLFTS ++K FQ+S+L +FF ++YFLGIITYGIA+PSGLFIPVILAGASYGR
Sbjct: 426 NTNDDAIRNLFTSGSEKSFQVSTLLVFFVAVYFLGIITYGIAIPSGLFIPVILAGASYGR 485

Query: 492 IVGRLFGSVATLDVSLYALLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLLMLVLLIS 551
           +VG+L G ++ LDVSL++LLGAASFLGGTMRMTVSLC+ILLELTN+LLMLPL+MLVLLIS
Sbjct: 486 LVGKLLGPISDLDVSLFSLLGAASFLGGTMRMTVSLCIILLELTNDLLMLPLMMLVLLIS 545

Query: 552 KSVADIFNKGVYDQIVKMKGLPFMEAHAEPFMRQLVAGGVASGPLITFSGIEKVDNIMHA 611
           K+VAD FNKGVYDQIVKMKGLP+MEAHAEP+MRQLVA  V SGPL++FSG+EKV NI+HA
Sbjct: 546 KTVADSFNKGVYDQIVKMKGLPYMEAHAEPYMRQLVASDVVSGPLVSFSGVEKVGNILHA 605

Query: 612 LKMTNHNGFPVIDEPPFSDSSELCGLVLRSHLLVLLKEKKFTKKKTSIRSEILRSFKAYD 671
           LK T HNGFPVIDEPPFSD+SELCGLVLRSHLLVLLK KKFTK +    S IL  FKA+D
Sbjct: 606 LKTTGHNGFPVIDEPPFSDASELCGLVLRSHLLVLLKGKKFTKLRIMSGSGILTIFKAHD 665

Query: 672 FAKPGSGKGVKLEDLDINEEEMEMFVDLHPITNTSPYTVVETMSLAKAAILFHALGLRHL 731
           FAK GSGKG+KLEDLDI  EEM+M+VDLHPITNTSPYTVVE+MSLAKAA+LF  LGLRHL
Sbjct: 666 FAKAGSGKGIKLEDLDITAEEMDMYVDLHPITNTSPYTVVESMSLAKAAVLFRELGLRHL 725

Query: 732 LVVPKTPGRPPIAGILTRHDFMPEYILGLYPYLNPH 736
            VVPKTPGRPPI GILTRHDF+PE+ILGLYP++NPH
Sbjct: 726 CVVPKTPGRPPIVGILTRHDFVPEHILGLYPHINPH 753