Cp4.1LG01g01810 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG01g01810 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 1.6e-243
Identity = 430/729 (58.98%), Postives = 534/729 (73.25%), Query Frame = 1

Query: 1   LVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPD 60
           L  F  F  + LAS LDS D+DG+ +YIVY+G K ED  S   HH  MLE+VVGSTFAP+
Sbjct: 9   LFFFSLFFSNRLASRLDS-DDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGSTFAPE 68

Query: 61  ALLHSYKRSFNGFVFIIW-----NVSAKEGVVSVFPNGKKHLHTTRSWDFMGFTKNVRRV 120
           ++LH+YKRSFNGF   +       +++ EGVVSVF N    LHTTRSWDF+GF   V R 
Sbjct: 69  SVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRR 128

Query: 121 KQVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSD 180
            QVESNIVVGVLD+GIWPESPSF D G+  PP KWKG C+TS NFRCNRKI+GAR+Y   
Sbjct: 129 SQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIG 188

Query: 181 KSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWS 240
           +   P D+  PRD++GHGTHTAST AGGLV+QA+LYGL LGTARGGVP ARIA YK+CW+
Sbjct: 189 RPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWN 248

Query: 241 DGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGN 300
           DGC D DILAA+DDAIADGVDIISLSVGG  P++YF D+IAIG+FH+++ GILTSNSAGN
Sbjct: 249 DGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGN 308

Query: 301 DGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAG 360
            GP++FT  + SPW LSVAAS++DRK V++VQ+GN   +QG +INTFD   + YPL+   
Sbjct: 309 GGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGR 368

Query: 361 NAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSP-STFASFSGAVGVVMNDAGV 420
           + PN   GF  S+SRFC+  SV+ NL++GKI++C++   P   F S  GA GV+M  +  
Sbjct: 369 DIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMT-SNT 428

Query: 421 KDNARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNP 480
           +D A SYPLPSS L P        Y+ S + P ATIFKS T+ + SAP++VSFSSRGPN 
Sbjct: 429 RDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNR 488

Query: 481 ETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYL- 540
            T D++KPD++ PGVEILAAW  +A V  G+R  R TL+NIISGTSMSCPH T  A Y+ 
Sbjct: 489 ATKDVIKPDISGPGVEILAAWPSVAPV-GGIR--RNTLFNIISGTSMSCPHITGIATYVK 548

Query: 541 --------------------------NPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVR 600
                                     NPQAEFAYG+GH+NP+KAV PGLVYDA ESDYV+
Sbjct: 549 TYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVK 608

Query: 601 FLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVG 660
           FLCGQGY T  VRR++GD+S CT  N+GRVWDLNYPSF LS +PS++ NQ+F RT+T+V 
Sbjct: 609 FLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVA 668

Query: 661 SKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAALLWTDGH 697
            + +TYRA ++  P+GLTISVNP VLSFN +G +KSFT+TVRG +   +VSA+L+W+DG 
Sbjct: 669 PQASTYRA-MISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGV 727

BLAST of Cp4.1LG01g01810 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 4.4e-169
Identity = 324/722 (44.88%), Postives = 446/722 (61.77%), Query Frame = 1

Query: 17  DSSDNDGRK---VYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGF 76
           D S ND R+   VYIVY+G  PE   S PSHH+ +L+++VG+  A   L+ SYKRSFNGF
Sbjct: 20  DVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGF 79

Query: 77  VFIIWNVSAKE-----GVVSVFPNGKKHLHTTRSWDFMGFTKNVRRVKQVESNIVVGVLD 136
              +    +++      VVSVFP+    L TTRSWDF+GF +  RR    ES+++VGV+D
Sbjct: 80  AANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVID 139

Query: 137 SGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDKSFPPEDIKSPRD 196
           SGIWPES SF D G+G PP KWKG+C+    F CN K++GAR Y            S RD
Sbjct: 140 SGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFAD-------SARD 199

Query: 197 SDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFD 256
            +GHGTHTAST AG  V  AS YGLA GTARGGVPSARIA YK+C++  C D DILAAFD
Sbjct: 200 EEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFNR-CNDVDILAAFD 259

Query: 257 DAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSP 316
           DAIADGVD+IS+S+  +      N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SP
Sbjct: 260 DAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSP 319

Query: 317 WSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSS 376
           W ++VAAS  DR+ + +V LGN     G ++NTF+L G ++P++Y     NVS   + + 
Sbjct: 320 WMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQ 379

Query: 377 SRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVKDNARSYPLPSSYL 436
           + +CS   VD  LV+GKI+LCD  L     A  +GA+GV++ +  + D+A   P P+S L
Sbjct: 380 AGYCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSL 439

Query: 437 GPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPG 496
           G     +IK+Y+ S + P A I ++  + D  AP + SFSSRGP+    ++LKPD++APG
Sbjct: 440 GFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPG 499

Query: 497 VEILAAWSPIASVSS--GVRDSRTTLYNIISGTSMSCPHATAAAVYL-------NPQAEF 556
           +EILAA+SP+AS SS     D R+  Y+++SGTSM+CPH    A Y+       +P A  
Sbjct: 500 LEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIK 559

Query: 557 --------------------AYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMV 616
                               AYG+G INP KA +PGLVY+ +  DY++ LC +G+ +  +
Sbjct: 560 SAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTL 619

Query: 617 RRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLG 676
              SG +  C+      V DLNYP+     +  +  N  F+RTVTNVG   +TY+A V+ 
Sbjct: 620 TTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVP 679

Query: 677 VPRGLTISVNPPVLSFNAIGQKKSFTVTVRGG--VNQPIVSAALLWTDGHHHVRSPITVY 700
           +   L IS+ P +L F  + +KKSF VT+ G    +   VS++++W+DG H VRSPI  Y
Sbjct: 680 LQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 727

BLAST of Cp4.1LG01g01810 vs. Swiss-Prot
Match: SBT44_ARATH (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 2.3e-165
Identity = 327/733 (44.61%), Postives = 457/733 (62.35%), Query Frame = 1

Query: 10  SLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSYKR 69
           SL + S D  D+  ++VYIVYLG+ P     TP S HM +L+E+ G +   + L+ SYK+
Sbjct: 18  SLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKK 77

Query: 70  SFNGFVFIIWNVSAK-----EGVVSVFPNGKKHLHTTRSWDFMGFTKNVR--RVKQVESN 129
           SFNGF   +     K     E VVSVFP+ K  L TT SW+FMG  + ++  R + +ES+
Sbjct: 78  SFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESD 137

Query: 130 IVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDKSFPPE 189
            ++GV+DSGI+PES SFSD G+G PP KWKG C    NF CN K++GAR Y +      +
Sbjct: 138 TIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS----K 197

Query: 190 DIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDA 249
             ++ RD  GHGTHTAS  AG  V  ++ YGL  GTARGGVP+ARIAVYK+C ++GC   
Sbjct: 198 ANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGE 257

Query: 250 DILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYF 309
            +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M  G+LT N+AGN+GP   
Sbjct: 258 AMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKIS 317

Query: 310 TIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVS 369
           T+ + +PW  SVAAS  +R  ++KV LG+  +  G ++NT+D+ G  YPL+Y  +A    
Sbjct: 318 TVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSA--AL 377

Query: 370 GGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVG-VVMNDAGVKDNARS 429
              +   +R C    +D  LV+GKI+LCDS       A   GAVG +V N    +   RS
Sbjct: 378 STCSVDKARLCEPKCLDGKLVKGKIVLCDS-TKGLIEAQKLGAVGSIVKNPEPDRAFIRS 437

Query: 430 YPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPETYDIL 489
           +P+  S+L      ++ +YM S K P AT+ KS  +++  APL+ SFSSRGP+    DIL
Sbjct: 438 FPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDIL 497

Query: 490 KPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYL---NPQ- 549
           KPD+TAPGVEILAA+SP +S +    D+R   Y+++SGTSM+CPH    A Y+   +PQ 
Sbjct: 498 KPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQW 557

Query: 550 -------------------------AEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCG 609
                                     EFAYG+GH++P+ A+NPGLVY+  ++D++ FLCG
Sbjct: 558 SPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCG 617

Query: 610 QGYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFRRTVTNVGSKV 669
             YT+  +R +SGD+S CT+  S  +  +LNYP+ +   + ++  N  F+RTVTNVG + 
Sbjct: 618 LNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQK 677

Query: 670 ATYRAKVLGVPRG-LTISVNPPVLSFNAIGQKKSFTVTVRG---GVNQPIVSAALLWTDG 700
           +TY AKV+  P   L+I V+P VLS  ++ +K+SF VTV     G  QP VSA L+W+DG
Sbjct: 678 STYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP-VSANLIWSDG 737

BLAST of Cp4.1LG01g01810 vs. Swiss-Prot
Match: SBT46_ARATH (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 6.6e-165
Identity = 331/733 (45.16%), Postives = 444/733 (60.57%), Query Frame = 1

Query: 10  SLLASSLDSS--DNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSY 69
           +LL  S  S+  D+  ++VYIVY+G  P      P SHH  +L++V G +   D L+ +Y
Sbjct: 15  ALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQDRLVRNY 74

Query: 70  KRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGFT--KNVRRVKQVE 129
           KRSFNGF   +       +++ + VVSVFP+   +L TT SW+FMG    K  +R   +E
Sbjct: 75  KRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIE 134

Query: 130 SNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDKSFP 189
           S+ ++GV+DSGI+PES SFS  G+G PP KWKG C+   NF CN K++GAR Y       
Sbjct: 135 SDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTPKLEGF 194

Query: 190 PEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSD--G 249
           PE   S RD+ GHG+HTAS  AG  V   S YGL  GT RGGVP+ARIAVYK+C      
Sbjct: 195 PE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIR 254

Query: 250 CYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDG 309
           C    ILAAFDDAIAD VDII++S+G +    +  D++AIGAFH+M  GILT N AGN+G
Sbjct: 255 CTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNG 314

Query: 310 PDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNA 369
           P+  TI + +PW  +VAAS+++R  ++KV LGN     G ++N+FDL GK+YPL+Y  +A
Sbjct: 315 PERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKSA 374

Query: 370 PNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVKDN 429
              S     SS+ FCS   +D   V+GKI+LCD+  +P    +      +V N    +D 
Sbjct: 375 ---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNP--YEDA 434

Query: 430 ARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPETY 489
           A  +  P S L     N + +Y+ S K P A + KS T+ +  AP++ S+SSRGPNP  +
Sbjct: 435 ASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIH 494

Query: 490 DILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYL---- 549
           DILKPD+TAPG EILAA+SP    S    D+R   Y +ISGTSMSCPH    A Y+    
Sbjct: 495 DILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTVISGTSMSCPHVAGVAAYIKTFH 554

Query: 550 --------------------------NPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVR 609
                                     N  AEFAYGAGH++P+ A++PGLVY+A +SD++ 
Sbjct: 555 PLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHIT 614

Query: 610 FLCGQGYTTAMVRRLSGDHSVCTRANSGRVW-DLNYPSFALSSTPSESINQFFRRTVTNV 669
           FLCG  YT   +R +SGD S CT+  +  +  +LNYPS +   + ++     FRRTVTNV
Sbjct: 615 FLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNV 674

Query: 670 GSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGG--VNQPIVSAALLWT 698
           G   ATY+AKV+G    L + V P VLS  ++ +KKSFTVTV G     + +VSA L+W+
Sbjct: 675 GRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWS 734

BLAST of Cp4.1LG01g01810 vs. Swiss-Prot
Match: SBT45_ARATH (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 9.5e-164
Identity = 334/736 (45.38%), Postives = 442/736 (60.05%), Query Frame = 1

Query: 10  SLLASSLDSSDNDG--RKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSY 69
           +LL  S  S+D D   ++ YIVY+G  P      P SHH  +L++V G +   D L+ +Y
Sbjct: 16  ALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSIEDRLVRNY 75

Query: 70  KRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGF--TKNVRRVKQVE 129
           KRSFNGF   +       +++ + VVSVFPN K  L TT SW+FMG   +K  +R   +E
Sbjct: 76  KRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKRNTIIE 135

Query: 130 SNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDKSFP 189
           S+ ++GV+DSGI+PES SFS  G+G PP KWKG C+   NF  N K++GAR Y       
Sbjct: 136 SDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYYTPKLEGF 195

Query: 190 PEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKIC--WSDG 249
           PE   S RD  GHG+HTAST AG  V   S YGL  GTARGGVP+ARIAVYK+C    DG
Sbjct: 196 PE---SARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGVDG 255

Query: 250 CYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDG 309
           C    ILAAFDDAIAD VDII++S+GG+    +  D IAIGAFH+M  GIL  NSAGN G
Sbjct: 256 CTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGNSG 315

Query: 310 PDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNA 369
           P+  T+ + +PW  +VAAS+ +R  V+KV LGN     G ++N+FDL GK+YPL+Y  +A
Sbjct: 316 PEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGKSA 375

Query: 370 PNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVKDN 429
            +  G    +S+ FCS   +D   V+GKI+LCDS  +P    +      +V +     D 
Sbjct: 376 SSSCGA---ASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHR--TDV 435

Query: 430 ARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPETY 489
           A  +  P S L     N + +YM S K P A + KS T+ +  AP++ S+ SRGPN    
Sbjct: 436 ASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIP 495

Query: 490 DILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYL---- 549
           DILKPD+TAPG EI+AA+SP A  S  + D+R   Y++ +GTSMSCPH    A YL    
Sbjct: 496 DILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 555

Query: 550 --------------------------NPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVR 609
                                     N  AEFAYGAGH++P+ A++PGLVY+A +SD++ 
Sbjct: 556 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 615

Query: 610 FLCGQGYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFRRTVTNV 669
           FLCG  YT   +R +SGD S CT+  +  +  +LNYPS     + ++     FRRTVTNV
Sbjct: 616 FLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNV 675

Query: 670 GSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGG--VNQPIVSAALLWT 701
           G   ATY+AKV+G    L + V P VLS  ++ +KKSFTVT  G     + +VSA L+W+
Sbjct: 676 GRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWS 735

BLAST of Cp4.1LG01g01810 vs. TrEMBL
Match: A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1)

HSP 1 Score: 1200.3 bits (3104), Expect = 0.0e+00
Identity = 596/704 (84.66%), Postives = 636/704 (90.34%), Query Frame = 1

Query: 31  LGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGFVFIIWN-----VSAKEG 90
           +GNK EDSASTPSHHMRMLEEVVGS+FAP+ALLHSYKRSFNGFV  +       +SAKE 
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 91  VVSVFPNGKKHLHTTRSWDFMGFTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGL 150
           VVSVFPN KKHLHTTRSWDFMGFT+   RVKQVESNIVVGVLDSGIWPESPSFSDVGYG 
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 151 PPAKWKGACQTSANFRCNRKIVGARAYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLV 210
           PP KWKGACQTSANF CNRKI+GARAYRSDK FPPEDIKSPRDSDGHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180

Query: 211 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN 270
           NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240

Query: 271 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSK 330
           KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS+
Sbjct: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300

Query: 331 VQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGK 390
           VQLGNKN +QGYTINTFDLKGKQ+PLIYAG+APN+S GFTGSSSRFCSRNSVDRNLV+GK
Sbjct: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360

Query: 391 ILLCDSILSPSTFASFSGAVGVVMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKF 450
           I+LCDS+LSP+TF S +GAVGVVMND GVKDNARSYPLPSSYL PV G+NIKTYM   +F
Sbjct: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420

Query: 451 PTATIFKSNTVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV 510
           PTATI KSN VNDTSAP IVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGV
Sbjct: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480

Query: 511 RDSRTTLYNIISGTSMSCPHATAAAVY---------------------------LNPQAE 570
           RDSRTTLYNIISGTSMSCPHATAAAVY                           LN Q E
Sbjct: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540

Query: 571 FAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVW 630
           FAYGAGHINP++AV+PGL+YDA ESDYVRFLCGQGYTTAMVRRLSGD+SVCTRANSGRVW
Sbjct: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600

Query: 631 DLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAI 690
           DLNYPSFALSST S+S NQFFRRTVTNVGSKV+TYRAKV+GVPRGL+I+VNPPVLSFNAI
Sbjct: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660

Query: 691 GQKKSFTVTVRGGVNQPIVSAALLWTDGHHHVRSPITVYVVDKA 703
           GQKKSFT+T+RG ++Q IVSA+L+W+DGHH+VRSPITV+VV  A
Sbjct: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 704

BLAST of Cp4.1LG01g01810 vs. TrEMBL
Match: A0A0A0KLY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1)

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 546/732 (74.59%), Postives = 610/732 (83.33%), Query Frame = 1

Query: 1   LVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPD 60
           LV+ +    SLLAS  DS  ND RK+YIVY+GNKP+D+ASTPSHHMRML EV GS FAP+
Sbjct: 11  LVLVLGLVSSLLASGFDSK-NDDRKIYIVYMGNKPQDTASTPSHHMRMLREVTGSNFAPE 70

Query: 61  ALLHSYKRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGFTKNVRRV 120
           +LLHSYKRSFNGFV  +       +SAKEGVVSVFP+GKKHLHTTRSWDF+GFTK+V RV
Sbjct: 71  SLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFIGFTKDVPRV 130

Query: 121 KQVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSD 180
            QVES+IVVGVLDSGIWPE+PSFSD GYG  PAKWKG CQ   NF CN+KI+GARAYRSD
Sbjct: 131 NQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKIIGARAYRSD 190

Query: 181 KSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWS 240
             FP EDI SPRDS+GHGTHTASTVAGGLV+QASLYGLALGTARGGVPSARIAVYKICWS
Sbjct: 191 NVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWS 250

Query: 241 DGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGN 300
           DGC DADILAAFDDAIADGVDIISLSVGG++ +YYFNDSIAIGAFHSMKHGILTSNSAGN
Sbjct: 251 DGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHGILTSNSAGN 310

Query: 301 DGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAG 360
           DGPDYFTIRNFSPWSLSVAAS+ DRKLVS+V++GN NVYQGYTINTFD  GKQYPLIYAG
Sbjct: 311 DGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLGKQYPLIYAG 370

Query: 361 NAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVK 420
           +APN+ GGFTGS SRFCS  SVD NLV GKILLCDSIL+PS F  FS AVGVVMND GVK
Sbjct: 371 DAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVGVVMNDDGVK 430

Query: 421 DNARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPE 480
             + SYPLPSSYL  V G+ IKTYM SN  PTATIFKS+ VND+SAP IVSFSSRGPNPE
Sbjct: 431 YPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSSAPFIVSFSSRGPNPE 490

Query: 481 TYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVY--- 540
           T DILKPDLTAPGVEILAAWSPIA VSSGV DSRTTLYNIISGTSMSCPH TAAAVY   
Sbjct: 491 TLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHVTAAAVYVKT 550

Query: 541 ------------------------LNPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRF 600
                                   +N +AEFAYGAG INP+KA++PGLVYDA E DYV+F
Sbjct: 551 FHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYDANEFDYVKF 610

Query: 601 LCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVGS 660
           LCGQGYT+ MV+ LS D+++C  AN GRVWDLNYPSFALSSTPS+SINQFF RT+T+V S
Sbjct: 611 LCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFFTRTLTSVDS 670

Query: 661 KVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVN-QPIVSAALLWTDGH 700
             +TY + +LG P+GLTI+VNP VLSF+ IG+KK+FT+T++G ++   IVSA+L+W+D  
Sbjct: 671 NASTYTSTILGAPQGLTITVNPKVLSFSGIGEKKTFTLTIQGTIDPTTIVSASLVWSDSS 730

BLAST of Cp4.1LG01g01810 vs. TrEMBL
Match: A0A0A0KNZ5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187870 PE=4 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 2.3e-294
Identity = 506/740 (68.38%), Postives = 594/740 (80.27%), Query Frame = 1

Query: 1   LVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNK-----PEDSASTPSHHMRMLEEVVGS 60
           +V+    SG L++ S   SD+D RKVYIVY+G+K      +D+ S PSHH R+LE+   S
Sbjct: 10  IVVLCLISGLLVSCS--GSDHDARKVYIVYMGDKLHDTDSDDTDSAPSHHKRILEKGTSS 69

Query: 61  TFAPDALLHSYKRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGFTK 120
            FAP+ LLHSYKRSFNGFV  +       +SA E VVS+FPN KKHLHTTRSWDF+G TK
Sbjct: 70  NFAPEFLLHSYKRSFNGFVAKLTEEEAQKISAMEEVVSLFPNEKKHLHTTRSWDFIGLTK 129

Query: 121 NVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGAR 180
           +  RVKQVESN+VVGV D+GIWPE+PSFSDVGYG  PAKWKG CQTSANF CN+KI+GAR
Sbjct: 130 DAPRVKQVESNLVVGVFDTGIWPENPSFSDVGYGPIPAKWKGTCQTSANFTCNKKIIGAR 189

Query: 181 AYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVY 240
           AYRS+  FPPEDI+SPRDSDGHGTHTASTV GGLVN+AS YGLA GTARGG PSA IAVY
Sbjct: 190 AYRSNNDFPPEDIRSPRDSDGHGTHTASTVVGGLVNEASFYGLAGGTARGGTPSACIAVY 249

Query: 241 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTS 300
           KICWSDGCY  DILAAFDDAIADGVD+IS+S+G  +   YF D  AIGAFH+MK+GILTS
Sbjct: 250 KICWSDGCYSTDILAAFDDAIADGVDMISISLGSPQSSPYFLDPTAIGAFHAMKNGILTS 309

Query: 301 NSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYP 360
            SAGN+GP+YF++ N +PW+LSV AS+IDRKL SKV+LGN+N+YQG+TINTFDL+GKQYP
Sbjct: 310 TSAGNEGPNYFSVSNVAPWALSVGASTIDRKLASKVELGNRNIYQGFTINTFDLEGKQYP 369

Query: 361 LIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMN 420
           LIYA +APN++GGFTGS SRFCS NSV+ NLV+GK+L+CDS+L PS F +FS AVGV+MN
Sbjct: 370 LIYARDAPNIAGGFTGSMSRFCSANSVNANLVKGKVLVCDSVLPPSRFVNFSDAVGVIMN 429

Query: 421 DAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSR 480
           D   KD++ SYPLPSSYL    GNN+KTYM SN  PTATI+KSN +NDTSAPL+VSFSSR
Sbjct: 430 DGRTKDSSGSYPLPSSYLTTADGNNVKTYMSSNGAPTATIYKSNAINDTSAPLVVSFSSR 489

Query: 481 GPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAA 540
           GPNP+T+DILKPDLTAPGV+ILAAWSPIA VSSGV DSRTTLYNIISGTSMSCPH TAAA
Sbjct: 490 GPNPQTFDILKPDLTAPGVQILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHVTAAA 549

Query: 541 VY---------------------------LNPQAEFAYGAGHINPVKAVNPGLVYDAQES 600
           VY                           LN QAEFAYGAG I+PVKA++PGLVYDA ES
Sbjct: 550 VYVKTFHPTWSPAAIQSALMTTATPLSAVLNMQAEFAYGAGQIDPVKAIDPGLVYDAGES 609

Query: 601 DYVRFLCGQGYTTAMVRRLSGD-HSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRT 660
           DYV+FLCGQGYTT+MV+R S D ++VC   N GRVWDLNYPSFALSS+PS   NQ+F RT
Sbjct: 610 DYVKFLCGQGYTTSMVQRFSNDKNTVCNSTNMGRVWDLNYPSFALSSSPSRPFNQYFTRT 669

Query: 661 VTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAALL 703
           +TNVGSK +TY + V G P+GLTI+VNP  LSFN+ GQK++FT+T+RG V+  I SA+L+
Sbjct: 670 LTNVGSKASTYTSTVRGTPQGLTITVNPTSLSFNSTGQKRNFTLTIRGTVSSSIASASLI 729

BLAST of Cp4.1LG01g01810 vs. TrEMBL
Match: K7NBW1_SIRGR (Cucumisin OS=Siraitia grosvenorii PE=2 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 1.1e-275
Identity = 488/730 (66.85%), Postives = 577/730 (79.04%), Query Frame = 1

Query: 2   VIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPDA 61
           +IF+S   SLL SS DS+D DGRK+YIVY+G+K ED+AS   +H  MLEEVVGSTFAP++
Sbjct: 13  LIFLSLFCSLLVSSSDSND-DGRKIYIVYMGSKLEDTASAHLYHRAMLEEVVGSTFAPES 72

Query: 62  LLHSYKRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGFTKNVRRVK 121
           ++++YKRSFNGF   +       ++AKEGVVSVFP+ K HLHTTRSWDF+G ++NV RVK
Sbjct: 73  VIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLGISQNVPRVK 132

Query: 122 QVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDK 181
           QVESNIVVGV DSGIWPE+PSF+D G+G  PA W+G CQ S NFRCNRKI+GARAYRS  
Sbjct: 133 QVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKIIGARAYRSS- 192

Query: 182 SFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSD 241
           + PP D++SPRD+DGHGTHTASTVAG LV+QASLYGL +GTARGGVP ARIAVYKICWSD
Sbjct: 193 TLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARIAVYKICWSD 252

Query: 242 GCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGND 301
           GC DADILAAFDDAIADGVDIISLSVGG  P+ Y  +SIAIG+FH+MK GILTSNSAGN+
Sbjct: 253 GCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGILTSNSAGNN 312

Query: 302 GPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGN 361
           GP  FT+ + SPW  +VAASS DRK V++V LGN N YQG +INTFD++  QYPLIYAGN
Sbjct: 313 GPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDMR-NQYPLIYAGN 372

Query: 362 APNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVKD 421
           AP++  GF  S+SR+C  +SVD NLVRGKILLCDS   P+ FASF GA GV+M  +  +D
Sbjct: 373 APSI--GFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVLM-QSNTRD 432

Query: 422 NARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPET 481
           +A SYPLP+S L P  GNNIK YM S + PTATIFKS  V DTSAP++VSFSSRGPN  T
Sbjct: 433 HASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSRGPNYVT 492

Query: 482 YDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYL--- 541
           +DILKPD TAPGVEILAAW P+A + SGVRDSR+ LYNIISGTSMSCPH TA AV++   
Sbjct: 493 HDILKPDSTAPGVEILAAWPPVAPI-SGVRDSRSALYNIISGTSMSCPHVTAIAVHIKTF 552

Query: 542 ------------------------NPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFL 601
                                   N  AEFAYG+GH+NP+KAV+PGLVYDA ESDYV+FL
Sbjct: 553 YPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDASESDYVKFL 612

Query: 602 CGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVGSK 661
           CG+GYTTAMVR  +GD+S CT  N GRVWDLNYPSFALS + S++ NQ FRRT+TNV S 
Sbjct: 613 CGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQTANQSFRRTLTNVVSG 672

Query: 662 VATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAALLWTDGHHH 700
            +TYRA +   P+GL+ISVNP VLSFN IG +KSFT+TVRG V+Q IVSA+L+W+DG H+
Sbjct: 673 ASTYRASI-SAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVSQAIVSASLVWSDGSHN 732

BLAST of Cp4.1LG01g01810 vs. TrEMBL
Match: D7TLL6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g02480 PE=4 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 7.7e-245
Identity = 435/701 (62.05%), Postives = 519/701 (74.04%), Query Frame = 1

Query: 31  LGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGFVFIIWNVSAKE-----G 90
           +G+KP    S  + H  ML++V GS  A D+LL+SYKRSFNGFV  +     KE     G
Sbjct: 1   MGDKPSGDISAVTAHTNMLQQVFGSNIASDSLLYSYKRSFNGFVVKLTEEEMKELEGMDG 60

Query: 91  VVSVFPNGKKHLHTTRSWDFMGFTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGL 150
           VVS+FPN KK LHTTRSWDF+GF + V R   VES++++ VLD+GIWPES SF D G+G 
Sbjct: 61  VVSIFPNEKKKLHTTRSWDFIGFPQQVNRT-SVESDVIIAVLDTGIWPESDSFKDKGFGP 120

Query: 151 PPAKWKGACQTSANFRCNRKIVGARAYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLV 210
           PP+KWKG CQ  +NF CN KI+GAR YRS   F PED+++PRDS+GHGTHTAST AGGLV
Sbjct: 121 PPSKWKGICQGLSNFTCNNKIIGARYYRSYGEFSPEDLQTPRDSEGHGTHTASTAAGGLV 180

Query: 211 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN 270
           + ASL G  LGTARGGVPSARIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG+
Sbjct: 181 SMASLLGFGLGTARGGVPSARIAVYKICWSDGCADADILAAFDDAIADGVDIISLSVGGS 240

Query: 271 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSK 330
            PK YF DSIAIGAFH+MK+GILTS SAGNDGP++ +I NFSPWSLSVAAS+IDRK  +K
Sbjct: 241 TPKNYFADSIAIGAFHAMKNGILTSTSAGNDGPNFASITNFSPWSLSVAASTIDRKFFTK 300

Query: 331 VQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGK 390
           VQLG+  VY+G +INTF+  G  YP IY G+APN++GGF+ ++SRFC+RNS+D NLV+GK
Sbjct: 301 VQLGDSKVYEGISINTFEPNG-MYPFIYGGDAPNITGGFSANTSRFCTRNSLDPNLVKGK 360

Query: 391 ILLCDSILSPSTFASFSGAVGVVMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKF 450
           I+LCD I S  T A  +GAVG VM D G KD+A  +PLP+SYLG   G++I  Y+ S   
Sbjct: 361 IVLCD-IFSNGTGAFLAGAVGTVMADRGAKDSAWPFPLPASYLGAQDGSSIAYYVTSTSN 420

Query: 451 PTATIFKSNTVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV 510
           PTA+I KS  VNDT AP IVSFSSRGPNP T DILKPDL APGV ILAAW PI+ +S   
Sbjct: 421 PTASILKSTEVNDTLAPFIVSFSSRGPNPATLDILKPDLAAPGVHILAAWPPISPISGVQ 480

Query: 511 RDSRTTLYNIISGTSMSCPHATAAAVYL---------------------------NPQAE 570
            D+R  LY + SGTSM+CPHAT AA Y+                           NP AE
Sbjct: 481 GDTRAVLYTMQSGTSMACPHATGAAAYIKSFHPTWSPAAIKSALMTTALPMSAEKNPDAE 540

Query: 571 FAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVW 630
           FAYGAG I+P+K+VNPGLVYDA + DYV+FLCGQGYTT  ++ ++GD+SVC+ A +G VW
Sbjct: 541 FAYGAGQIDPLKSVNPGLVYDADKIDYVKFLCGQGYTTQTLQLVTGDNSVCSEATNGTVW 600

Query: 631 DLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAI 690
           DLNYPSFALSS+  ESI   F RTVTNVGS V+TY+A V G P GL I V P +LSF ++
Sbjct: 601 DLNYPSFALSSSTFESITGVFTRTVTNVGSPVSTYKATVTGAPIGLQIQVVPDILSFTSL 660

Query: 691 GQKKSFTVTVRGGVNQPIVSAALLWTDGHHHVRSPITVYVV 700
           GQK SF + V G V   IVSA+L+W DG H VRSPI V ++
Sbjct: 661 GQKLSFVLKVEGKVGDNIVSASLVWDDGVHQVRSPIVVSIL 698

BLAST of Cp4.1LG01g01810 vs. TAIR10
Match: AT5G59100.1 (AT5G59100.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 583.9 bits (1504), Expect = 1.3e-166
Identity = 327/733 (44.61%), Postives = 457/733 (62.35%), Query Frame = 1

Query: 10  SLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSYKR 69
           SL + S D  D+  ++VYIVYLG+ P     TP S HM +L+E+ G +   + L+ SYK+
Sbjct: 18  SLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKK 77

Query: 70  SFNGFVFIIWNVSAK-----EGVVSVFPNGKKHLHTTRSWDFMGFTKNVR--RVKQVESN 129
           SFNGF   +     K     E VVSVFP+ K  L TT SW+FMG  + ++  R + +ES+
Sbjct: 78  SFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESD 137

Query: 130 IVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDKSFPPE 189
            ++GV+DSGI+PES SFSD G+G PP KWKG C    NF CN K++GAR Y +      +
Sbjct: 138 TIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS----K 197

Query: 190 DIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDA 249
             ++ RD  GHGTHTAS  AG  V  ++ YGL  GTARGGVP+ARIAVYK+C ++GC   
Sbjct: 198 ANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGE 257

Query: 250 DILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYF 309
            +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M  G+LT N+AGN+GP   
Sbjct: 258 AMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKIS 317

Query: 310 TIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVS 369
           T+ + +PW  SVAAS  +R  ++KV LG+  +  G ++NT+D+ G  YPL+Y  +A    
Sbjct: 318 TVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSA--AL 377

Query: 370 GGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVG-VVMNDAGVKDNARS 429
              +   +R C    +D  LV+GKI+LCDS       A   GAVG +V N    +   RS
Sbjct: 378 STCSVDKARLCEPKCLDGKLVKGKIVLCDS-TKGLIEAQKLGAVGSIVKNPEPDRAFIRS 437

Query: 430 YPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPETYDIL 489
           +P+  S+L      ++ +YM S K P AT+ KS  +++  APL+ SFSSRGP+    DIL
Sbjct: 438 FPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDIL 497

Query: 490 KPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYL---NPQ- 549
           KPD+TAPGVEILAA+SP +S +    D+R   Y+++SGTSM+CPH    A Y+   +PQ 
Sbjct: 498 KPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQW 557

Query: 550 -------------------------AEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCG 609
                                     EFAYG+GH++P+ A+NPGLVY+  ++D++ FLCG
Sbjct: 558 SPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCG 617

Query: 610 QGYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFRRTVTNVGSKV 669
             YT+  +R +SGD+S CT+  S  +  +LNYP+ +   + ++  N  F+RTVTNVG + 
Sbjct: 618 LNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQK 677

Query: 670 ATYRAKVLGVPRG-LTISVNPPVLSFNAIGQKKSFTVTVRG---GVNQPIVSAALLWTDG 700
           +TY AKV+  P   L+I V+P VLS  ++ +K+SF VTV     G  QP VSA L+W+DG
Sbjct: 678 STYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP-VSANLIWSDG 737

BLAST of Cp4.1LG01g01810 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 583.2 bits (1502), Expect = 2.2e-166
Identity = 314/705 (44.54%), Postives = 435/705 (61.70%), Query Frame = 1

Query: 31  LGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGFVFIIWNVSAKE-----G 90
           +G  PE   S PSHH+ +L+++VG+  A   L+ SYKRSFNGF   +    +++      
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 91  VVSVFPNGKKHLHTTRSWDFMGFTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGL 150
           VVSVFP+    L TTRSWDF+GF +  RR    ES+++VGV+DSGIWPES SF D G+G 
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 151 PPAKWKGACQTSANFRCNRKIVGARAYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLV 210
           PP KWKG+C+    F CN K++GAR Y            S RD +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAV 180

Query: 211 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN 270
             AS YGLA GTARGGVPSARIA YK+C++  C D DILAAFDDAIADGVD+IS+S+  +
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCFNR-CNDVDILAAFDDAIADGVDVISISISAD 240

Query: 271 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSK 330
                 N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SPW ++VAAS  DR+ + +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 331 VQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGK 390
           V LGN     G ++NTF+L G ++P++Y     NVS   + + + +CS   VD  LV+GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 391 ILLCDSILSPSTFASFSGAVGVVMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKF 450
           I+LCD  L     A  +GA+GV++ +  + D+A   P P+S LG     +IK+Y+ S + 
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 451 PTATIFKSNTVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSS-- 510
           P A I ++  + D  AP + SFSSRGP+    ++LKPD++APG+EILAA+SP+AS SS  
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 511 GVRDSRTTLYNIISGTSMSCPHATAAAVYL-------NPQAEF----------------- 570
              D R+  Y+++SGTSM+CPH    A Y+       +P A                   
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 571 ---AYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGR 630
              AYG+G INP KA +PGLVY+ +  DY++ LC +G+ +  +   SG +  C+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600

Query: 631 VWDLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFN 690
           V DLNYP+     +  +  N  F+RTVTNVG   +TY+A V+ +   L IS+ P +L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 691 AIGQKKSFTVTVRGG--VNQPIVSAALLWTDGHHHVRSPITVYVV 700
            + +KKSF VT+ G    +   VS++++W+DG H VRSPI  Y +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of Cp4.1LG01g01810 vs. TAIR10
Match: AT3G46850.1 (AT3G46850.1 Subtilase family protein)

HSP 1 Score: 582.4 bits (1500), Expect = 3.7e-166
Identity = 331/733 (45.16%), Postives = 444/733 (60.57%), Query Frame = 1

Query: 10  SLLASSLDSS--DNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSY 69
           +LL  S  S+  D+  ++VYIVY+G  P      P SHH  +L++V G +   D L+ +Y
Sbjct: 15  ALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQDRLVRNY 74

Query: 70  KRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGFT--KNVRRVKQVE 129
           KRSFNGF   +       +++ + VVSVFP+   +L TT SW+FMG    K  +R   +E
Sbjct: 75  KRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIE 134

Query: 130 SNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDKSFP 189
           S+ ++GV+DSGI+PES SFS  G+G PP KWKG C+   NF CN K++GAR Y       
Sbjct: 135 SDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTPKLEGF 194

Query: 190 PEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSD--G 249
           PE   S RD+ GHG+HTAS  AG  V   S YGL  GT RGGVP+ARIAVYK+C      
Sbjct: 195 PE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIR 254

Query: 250 CYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDG 309
           C    ILAAFDDAIAD VDII++S+G +    +  D++AIGAFH+M  GILT N AGN+G
Sbjct: 255 CTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNG 314

Query: 310 PDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNA 369
           P+  TI + +PW  +VAAS+++R  ++KV LGN     G ++N+FDL GK+YPL+Y  +A
Sbjct: 315 PERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKSA 374

Query: 370 PNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVKDN 429
              S     SS+ FCS   +D   V+GKI+LCD+  +P    +      +V N    +D 
Sbjct: 375 ---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNP--YEDA 434

Query: 430 ARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPETY 489
           A  +  P S L     N + +Y+ S K P A + KS T+ +  AP++ S+SSRGPNP  +
Sbjct: 435 ASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIH 494

Query: 490 DILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYL---- 549
           DILKPD+TAPG EILAA+SP    S    D+R   Y +ISGTSMSCPH    A Y+    
Sbjct: 495 DILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTVISGTSMSCPHVAGVAAYIKTFH 554

Query: 550 --------------------------NPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVR 609
                                     N  AEFAYGAGH++P+ A++PGLVY+A +SD++ 
Sbjct: 555 PLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHIT 614

Query: 610 FLCGQGYTTAMVRRLSGDHSVCTRANSGRVW-DLNYPSFALSSTPSESINQFFRRTVTNV 669
           FLCG  YT   +R +SGD S CT+  +  +  +LNYPS +   + ++     FRRTVTNV
Sbjct: 615 FLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNV 674

Query: 670 GSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGG--VNQPIVSAALLWT 698
           G   ATY+AKV+G    L + V P VLS  ++ +KKSFTVTV G     + +VSA L+W+
Sbjct: 675 GRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWS 734

BLAST of Cp4.1LG01g01810 vs. TAIR10
Match: AT3G46840.1 (AT3G46840.1 Subtilase family protein)

HSP 1 Score: 578.6 bits (1490), Expect = 5.4e-165
Identity = 334/736 (45.38%), Postives = 442/736 (60.05%), Query Frame = 1

Query: 10  SLLASSLDSSDNDG--RKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSY 69
           +LL  S  S+D D   ++ YIVY+G  P      P SHH  +L++V G +   D L+ +Y
Sbjct: 16  ALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSIEDRLVRNY 75

Query: 70  KRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGF--TKNVRRVKQVE 129
           KRSFNGF   +       +++ + VVSVFPN K  L TT SW+FMG   +K  +R   +E
Sbjct: 76  KRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKRNTIIE 135

Query: 130 SNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDKSFP 189
           S+ ++GV+DSGI+PES SFS  G+G PP KWKG C+   NF  N K++GAR Y       
Sbjct: 136 SDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYYTPKLEGF 195

Query: 190 PEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKIC--WSDG 249
           PE   S RD  GHG+HTAST AG  V   S YGL  GTARGGVP+ARIAVYK+C    DG
Sbjct: 196 PE---SARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGVDG 255

Query: 250 CYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDG 309
           C    ILAAFDDAIAD VDII++S+GG+    +  D IAIGAFH+M  GIL  NSAGN G
Sbjct: 256 CTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGNSG 315

Query: 310 PDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNA 369
           P+  T+ + +PW  +VAAS+ +R  V+KV LGN     G ++N+FDL GK+YPL+Y  +A
Sbjct: 316 PEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGKSA 375

Query: 370 PNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVKDN 429
            +  G    +S+ FCS   +D   V+GKI+LCDS  +P    +      +V +     D 
Sbjct: 376 SSSCGA---ASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHR--TDV 435

Query: 430 ARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPETY 489
           A  +  P S L     N + +YM S K P A + KS T+ +  AP++ S+ SRGPN    
Sbjct: 436 ASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIP 495

Query: 490 DILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYL---- 549
           DILKPD+TAPG EI+AA+SP A  S  + D+R   Y++ +GTSMSCPH    A YL    
Sbjct: 496 DILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 555

Query: 550 --------------------------NPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVR 609
                                     N  AEFAYGAGH++P+ A++PGLVY+A +SD++ 
Sbjct: 556 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 615

Query: 610 FLCGQGYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFRRTVTNV 669
           FLCG  YT   +R +SGD S CT+  +  +  +LNYPS     + ++     FRRTVTNV
Sbjct: 616 FLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNV 675

Query: 670 GSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGG--VNQPIVSAALLWT 701
           G   ATY+AKV+G    L + V P VLS  ++ +KKSFTVT  G     + +VSA L+W+
Sbjct: 676 GRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWS 735

BLAST of Cp4.1LG01g01810 vs. TAIR10
Match: AT5G59090.1 (AT5G59090.1 subtilase 4.12)

HSP 1 Score: 571.2 bits (1471), Expect = 8.6e-163
Identity = 322/738 (43.63%), Postives = 454/738 (61.52%), Query Frame = 1

Query: 11  LLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSYKRS 70
           LL SS+ +  ++  +VYIVY+G+    +   P S HM +L++V G +     L+ SYKRS
Sbjct: 17  LLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRS 76

Query: 71  FNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGFT--KNVRRVKQVESNI 130
           FNGF   +       ++  EGVVSVFPN    LHTT SWDFMG    KN +R   +ES+ 
Sbjct: 77  FNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDT 136

Query: 131 VVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDKSFPPED 190
           ++GV+D+GIWPES SFSD G+G PP KWKG C    NF CN K++GAR Y S+ +     
Sbjct: 137 IIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEGT----- 196

Query: 191 IKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDAD 250
               RD+ GHGTHTAST AG  V   S +G+  GT RGGVP++RIA YK+C   GC    
Sbjct: 197 ----RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEA 256

Query: 251 ILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFT 310
           +L++FDDAIADGVD+I++S+G   P  + +D IAIGAFH+M  GILT +SAGN GP   T
Sbjct: 257 LLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTT 316

Query: 311 IRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSG 370
           + + +PW  +VAAS+ +R  ++KV LGN     G ++N FD+KGK+YPL+Y  +A   S 
Sbjct: 317 VSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSA--ASS 376

Query: 371 GFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTF--ASFSGAVGVVMNDAGVKDNARS 430
                ++  C+   ++++ V+GKIL+C     PS +  A   GA+ ++ + +   D A +
Sbjct: 377 ACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKIAKSVGAIAII-DKSPRPDVAFT 436

Query: 431 YPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPETYDIL 490
           + LP+S L      ++ +Y+ S   P A + K+ T+ + ++P+I SFSSRGPN    DIL
Sbjct: 437 HHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDIL 496

Query: 491 KPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYLN------ 550
           KPD+TAPGVEILAA+SP    S    D+R   Y++ SGTSM+CPH    A Y+       
Sbjct: 497 KPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRW 556

Query: 551 -----------------------PQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCG 610
                                     EFAYGAGH++P+ A+NPGLVY+  ++D++ FLCG
Sbjct: 557 SPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCG 616

Query: 611 QGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFA--LSSTPSESINQFFRRTVTNVGSK 670
             YT+  ++ +SGD   C++ N     +LNYPS +  LS T S + +  F RT+TNVG+ 
Sbjct: 617 MNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDS-TFSVTFNRTLTNVGTP 676

Query: 671 VATYRAKVL-GVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGG-VNQPIVSAA-LLWTDG 703
            +TY++KV+ G    L+I V P VL F  + +K+SF+VTV G  V+  + S+A L+W+DG
Sbjct: 677 NSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDG 736

BLAST of Cp4.1LG01g01810 vs. NCBI nr
Match: gi|659123579|ref|XP_008461729.1| (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 621/733 (84.72%), Postives = 662/733 (90.31%), Query Frame = 1

Query: 2   VIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPDA 61
           V+F S   SLLAS LDS DNDGRK+YIVY+GNKPEDSASTPSHHMRMLEEVVGS+FAP+A
Sbjct: 36  VVFSSLFCSLLASGLDS-DNDGRKIYIVYMGNKPEDSASTPSHHMRMLEEVVGSSFAPEA 95

Query: 62  LLHSYKRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGFTKNVRRVK 121
           LLHSYKRSFNGFV  +       +SAKE VVSVFPN KKHLHTTRSWDFMGFT+NV RVK
Sbjct: 96  LLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGFTQNVPRVK 155

Query: 122 QVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDK 181
           QVESNIVVGVLDSGIWPESPSFSDVGYG PPAKWKGACQTSANFRCNRKI+GARAYRSDK
Sbjct: 156 QVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGARAYRSDK 215

Query: 182 SFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSD 241
            FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSARIAVYKICWSD
Sbjct: 216 FFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSARIAVYKICWSD 275

Query: 242 GCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGND 301
           GCYDADILAAFDDAIADGVDIISLSVGGN+PKYYFNDSIAIGAFHSMKHGILTSNSAGND
Sbjct: 276 GCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHGILTSNSAGND 335

Query: 302 GPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGN 361
           GPDYFTIRNFSPWSLSVAASSIDRKLVS+VQLGNKN+YQGYTINTFDLKGKQYPLIYAG+
Sbjct: 336 GPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKGKQYPLIYAGS 395

Query: 362 APNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVKD 421
           APN+SGGFTGSSSRFCSRNSVDRNLV+GKI+LCDS+LSP+TF S +GA+GVVMND GVKD
Sbjct: 396 APNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMGVVMNDLGVKD 455

Query: 422 NARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPET 481
           NARSYPLPSSYL PV GNNIKTYM  NKFPTATI KSN VNDTSAP IVSFSSRGPNPET
Sbjct: 456 NARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVSFSSRGPNPET 515

Query: 482 YDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVY---- 541
           YDILKPDLTAPGVEILAAWSPIA+VSSGVRDSRTTLYNIISGTSMSCPHATAAAVY    
Sbjct: 516 YDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYVKTF 575

Query: 542 -----------------------LNPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFL 601
                                  LN Q EFAYGAGHINP+KAV+PGL+YDA E DYVRFL
Sbjct: 576 HPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYDAYERDYVRFL 635

Query: 602 CGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVGSK 661
           CGQGYTTAMVRRLSGD+S C+RANSGRVWDLNYPSFALSST ++S NQFFRRTVTNVGSK
Sbjct: 636 CGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFFRRTVTNVGSK 695

Query: 662 VATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAALLWTDGHHH 703
           V+TYRAKV+G PRGL+I+VNPPVLSFNAIGQKKSFT+T+RG + Q IVSA+L+W+DG+H+
Sbjct: 696 VSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSASLMWSDGYHN 755

BLAST of Cp4.1LG01g01810 vs. NCBI nr
Match: gi|778701133|ref|XP_004149947.2| (PREDICTED: cucumisin-like [Cucumis sativus])

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 618/733 (84.31%), Postives = 659/733 (89.90%), Query Frame = 1

Query: 2   VIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPDA 61
           V F S   SLLASSLDS DNDGRK+YIVY+GNK EDSASTPSHHMRMLEEVVGS+FAP+A
Sbjct: 19  VFFSSLFSSLLASSLDS-DNDGRKIYIVYMGNKLEDSASTPSHHMRMLEEVVGSSFAPEA 78

Query: 62  LLHSYKRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGFTKNVRRVK 121
           LLHSYKRSFNGFV  +       +SAKE VVSVFPN KKHLHTTRSWDFMGFT+   RVK
Sbjct: 79  LLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGFTQKAPRVK 138

Query: 122 QVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSDK 181
           QVESNIVVGVLDSGIWPESPSFSDVGYG PP KWKGACQTSANF CNRKI+GARAYRSDK
Sbjct: 139 QVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQTSANFHCNRKIIGARAYRSDK 198

Query: 182 SFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSD 241
            FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSD
Sbjct: 199 FFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSD 258

Query: 242 GCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGND 301
           GCYDADILAAFDDAIADGVDIISLSVGG+KPKYYFNDSIAIGAFHSMKHGILTSNSAGND
Sbjct: 259 GCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTSNSAGND 318

Query: 302 GPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGN 361
           GPDYFTIRNFSPWSLSVAASSIDRKLVS+VQLGNKN +QGYTINTFDLKGKQ+PLIYAG+
Sbjct: 319 GPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNTFQGYTINTFDLKGKQHPLIYAGS 378

Query: 362 APNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVKD 421
           APN+S GFTGSSSRFCSRNSVDRNLV+GKI+LCDS+LSP+TF S +GAVGVVMND GVKD
Sbjct: 379 APNISAGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAVGVVMNDLGVKD 438

Query: 422 NARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPET 481
           NARSYPLPSSYL PV G+NIKTYM   +FPTATI KSN VNDTSAP IVSFSSRGPNPET
Sbjct: 439 NARSYPLPSSYLDPVDGDNIKTYMDRTRFPTATILKSNAVNDTSAPWIVSFSSRGPNPET 498

Query: 482 YDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVY---- 541
           YDILKPDLTAPGVEILAAWSPIA+VSSGVRDSRTTLYNIISGTSMSCPHATAAAVY    
Sbjct: 499 YDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATAAAVYVKTF 558

Query: 542 -----------------------LNPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFL 601
                                  LN Q EFAYGAGHINP++AV+PGL+YDA ESDYVRFL
Sbjct: 559 HPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLRAVHPGLLYDAYESDYVRFL 618

Query: 602 CGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVGSK 661
           CGQGYTTAMVRRLSGD+SVCTRANSGRVWDLNYPSFALSST S+S NQFFRRTVTNVGSK
Sbjct: 619 CGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTSSQSFNQFFRRTVTNVGSK 678

Query: 662 VATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAALLWTDGHHH 703
           V+TYRAKV+GVPRGL+I+VNPPVLSFNAIGQKKSFT+T+RG ++Q IVSA+L+W+DGHH+
Sbjct: 679 VSTYRAKVVGVPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSISQSIVSASLVWSDGHHN 738

BLAST of Cp4.1LG01g01810 vs. NCBI nr
Match: gi|700195413|gb|KGN50590.1| (hypothetical protein Csa_5G187880 [Cucumis sativus])

HSP 1 Score: 1200.3 bits (3104), Expect = 0.0e+00
Identity = 596/704 (84.66%), Postives = 636/704 (90.34%), Query Frame = 1

Query: 31  LGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGFVFIIWN-----VSAKEG 90
           +GNK EDSASTPSHHMRMLEEVVGS+FAP+ALLHSYKRSFNGFV  +       +SAKE 
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 91  VVSVFPNGKKHLHTTRSWDFMGFTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGL 150
           VVSVFPN KKHLHTTRSWDFMGFT+   RVKQVESNIVVGVLDSGIWPESPSFSDVGYG 
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 151 PPAKWKGACQTSANFRCNRKIVGARAYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLV 210
           PP KWKGACQTSANF CNRKI+GARAYRSDK FPPEDIKSPRDSDGHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180

Query: 211 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN 270
           NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240

Query: 271 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSK 330
           KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS+
Sbjct: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300

Query: 331 VQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGK 390
           VQLGNKN +QGYTINTFDLKGKQ+PLIYAG+APN+S GFTGSSSRFCSRNSVDRNLV+GK
Sbjct: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360

Query: 391 ILLCDSILSPSTFASFSGAVGVVMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKF 450
           I+LCDS+LSP+TF S +GAVGVVMND GVKDNARSYPLPSSYL PV G+NIKTYM   +F
Sbjct: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420

Query: 451 PTATIFKSNTVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV 510
           PTATI KSN VNDTSAP IVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGV
Sbjct: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480

Query: 511 RDSRTTLYNIISGTSMSCPHATAAAVY---------------------------LNPQAE 570
           RDSRTTLYNIISGTSMSCPHATAAAVY                           LN Q E
Sbjct: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540

Query: 571 FAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVW 630
           FAYGAGHINP++AV+PGL+YDA ESDYVRFLCGQGYTTAMVRRLSGD+SVCTRANSGRVW
Sbjct: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600

Query: 631 DLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAI 690
           DLNYPSFALSST S+S NQFFRRTVTNVGSKV+TYRAKV+GVPRGL+I+VNPPVLSFNAI
Sbjct: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660

Query: 691 GQKKSFTVTVRGGVNQPIVSAALLWTDGHHHVRSPITVYVVDKA 703
           GQKKSFT+T+RG ++Q IVSA+L+W+DGHH+VRSPITV+VV  A
Sbjct: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 704

BLAST of Cp4.1LG01g01810 vs. NCBI nr
Match: gi|659123581|ref|XP_008461730.1| (PREDICTED: cucumisin-like isoform X2 [Cucumis melo])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 578/683 (84.63%), Postives = 617/683 (90.34%), Query Frame = 1

Query: 52  VVGSTFAPDALLHSYKRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFM 111
           +V S+FAP+ALLHSYKRSFNGFV  +       +SAKE VVSVFPN KKHLHTTRSWDFM
Sbjct: 2   MVESSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFM 61

Query: 112 GFTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKI 171
           GFT+NV RVKQVESNIVVGVLDSGIWPESPSFSDVGYG PPAKWKGACQTSANFRCNRKI
Sbjct: 62  GFTQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKI 121

Query: 172 VGARAYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSAR 231
           +GARAYRSDK FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSAR
Sbjct: 122 IGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSAR 181

Query: 232 IAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHG 291
           IAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN+PKYYFNDSIAIGAFHSMKHG
Sbjct: 182 IAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHG 241

Query: 292 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKG 351
           ILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS+VQLGNKN+YQGYTINTFDLKG
Sbjct: 242 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKG 301

Query: 352 KQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVG 411
           KQYPLIYAG+APN+SGGFTGSSSRFCSRNSVDRNLV+GKI+LCDS+LSP+TF S +GA+G
Sbjct: 302 KQYPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMG 361

Query: 412 VVMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVS 471
           VVMND GVKDNARSYPLPSSYL PV GNNIKTYM  NKFPTATI KSN VNDTSAP IVS
Sbjct: 362 VVMNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVS 421

Query: 472 FSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHA 531
           FSSRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGVRDSRTTLYNIISGTSMSCPHA
Sbjct: 422 FSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHA 481

Query: 532 TAAAVY---------------------------LNPQAEFAYGAGHINPVKAVNPGLVYD 591
           TAAAVY                           LN Q EFAYGAGHINP+KAV+PGL+YD
Sbjct: 482 TAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYD 541

Query: 592 AQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFF 651
           A E DYVRFLCGQGYTTAMVRRLSGD+S C+RANSGRVWDLNYPSFALSST ++S NQFF
Sbjct: 542 AYERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFF 601

Query: 652 RRTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSA 703
           RRTVTNVGSKV+TYRAKV+G PRGL+I+VNPPVLSFNAIGQKKSFT+T+RG + Q IVSA
Sbjct: 602 RRTVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSA 661

BLAST of Cp4.1LG01g01810 vs. NCBI nr
Match: gi|700195411|gb|KGN50588.1| (hypothetical protein Csa_5G187860 [Cucumis sativus])

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 546/732 (74.59%), Postives = 610/732 (83.33%), Query Frame = 1

Query: 1   LVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPD 60
           LV+ +    SLLAS  DS  ND RK+YIVY+GNKP+D+ASTPSHHMRML EV GS FAP+
Sbjct: 11  LVLVLGLVSSLLASGFDSK-NDDRKIYIVYMGNKPQDTASTPSHHMRMLREVTGSNFAPE 70

Query: 61  ALLHSYKRSFNGFVFIIWN-----VSAKEGVVSVFPNGKKHLHTTRSWDFMGFTKNVRRV 120
           +LLHSYKRSFNGFV  +       +SAKEGVVSVFP+GKKHLHTTRSWDF+GFTK+V RV
Sbjct: 71  SLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFIGFTKDVPRV 130

Query: 121 KQVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIVGARAYRSD 180
            QVES+IVVGVLDSGIWPE+PSFSD GYG  PAKWKG CQ   NF CN+KI+GARAYRSD
Sbjct: 131 NQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKIIGARAYRSD 190

Query: 181 KSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWS 240
             FP EDI SPRDS+GHGTHTASTVAGGLV+QASLYGLALGTARGGVPSARIAVYKICWS
Sbjct: 191 NVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWS 250

Query: 241 DGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGN 300
           DGC DADILAAFDDAIADGVDIISLSVGG++ +YYFNDSIAIGAFHSMKHGILTSNSAGN
Sbjct: 251 DGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHGILTSNSAGN 310

Query: 301 DGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAG 360
           DGPDYFTIRNFSPWSLSVAAS+ DRKLVS+V++GN NVYQGYTINTFD  GKQYPLIYAG
Sbjct: 311 DGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLGKQYPLIYAG 370

Query: 361 NAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGVVMNDAGVK 420
           +APN+ GGFTGS SRFCS  SVD NLV GKILLCDSIL+PS F  FS AVGVVMND GVK
Sbjct: 371 DAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVGVVMNDDGVK 430

Query: 421 DNARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSFSSRGPNPE 480
             + SYPLPSSYL  V G+ IKTYM SN  PTATIFKS+ VND+SAP IVSFSSRGPNPE
Sbjct: 431 YPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSSAPFIVSFSSRGPNPE 490

Query: 481 TYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHATAAAVY--- 540
           T DILKPDLTAPGVEILAAWSPIA VSSGV DSRTTLYNIISGTSMSCPH TAAAVY   
Sbjct: 491 TLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHVTAAAVYVKT 550

Query: 541 ------------------------LNPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRF 600
                                   +N +AEFAYGAG INP+KA++PGLVYDA E DYV+F
Sbjct: 551 FHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYDANEFDYVKF 610

Query: 601 LCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVGS 660
           LCGQGYT+ MV+ LS D+++C  AN GRVWDLNYPSFALSSTPS+SINQFF RT+T+V S
Sbjct: 611 LCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFFTRTLTSVDS 670

Query: 661 KVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVN-QPIVSAALLWTDGH 700
             +TY + +LG P+GLTI+VNP VLSF+ IG+KK+FT+T++G ++   IVSA+L+W+D  
Sbjct: 671 NASTYTSTILGAPQGLTITVNPKVLSFSGIGEKKTFTLTIQGTIDPTTIVSASLVWSDSS 730