Cp4.1LG00g08780 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG00g08780
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCalcium-binding EF-hand family protein, putative
LocationCp4.1LG00 : 27682651 .. 27682929 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAATTCCAAAAGTAAAACCTACAAACGAGTTCTGGTTCCAGTCTCGGAAGAACAATTATTGGATATATTCAAGAGCCATGATCAGGACGGAGACGGAAAGCTATCAAAGGAGGAATTGAAGCAAGCCTTCAGCTATCTTGGATCACGTTTCAGTGCCTTTAGAGCTGAAGAAGCCCTACGCACCGTTGATGATAACCATGACGGTGTTGTCAGCATGGACGAGATGAACAAGCTAATTCAATATACCAAAGGTCGTAAATACACTATTTGTTGA

mRNA sequence

ATGGGGAATTCCAAAAGTAAAACCTACAAACGAGTTCTGGTTCCAGTCTCGGAAGAACAATTATTGGATATATTCAAGAGCCATGATCAGGACGGAGACGGAAAGCTATCAAAGGAGGAATTGAAGCAAGCCTTCAGCTATCTTGGATCACGTTTCAGTGCCTTTAGAGCTGAAGAAGCCCTACGCACCGTTGATGATAACCATGACGGTGTTGTCAGCATGGACGAGATGAACAAGCTAATTCAATATACCAAAGGTCGTAAATACACTATTTGTTGA

Coding sequence (CDS)

ATGGGGAATTCCAAAAGTAAAACCTACAAACGAGTTCTGGTTCCAGTCTCGGAAGAACAATTATTGGATATATTCAAGAGCCATGATCAGGACGGAGACGGAAAGCTATCAAAGGAGGAATTGAAGCAAGCCTTCAGCTATCTTGGATCACGTTTCAGTGCCTTTAGAGCTGAAGAAGCCCTACGCACCGTTGATGATAACCATGACGGTGTTGTCAGCATGGACGAGATGAACAAGCTAATTCAATATACCAAAGGTCGTAAATACACTATTTGTTGA

Protein sequence

MGNSKSKTYKRVLVPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVSMDEMNKLIQYTKGRKYTIC
Homology
[Close]
BLAST of Cp4.1LG00g08780 vs. Swiss-Prot
Match: CML15_ARATH (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana GN=CML15 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 6.9e-06
Identity = 26/68 (38.24%), Postives = 40/68 (58.82%), Query Frame = 1 

Query: 14  VPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVS 73
           V ++ EQLL+IFKS D+DG+G +S  EL  A + +G   +     E ++  D N DGV+S
Sbjct: 76  VLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEADTNGDGVIS 135

Query: 74  MDEMNKLI 82
             E   ++
Sbjct: 136 FGEFASIM 143

[Close]
BLAST of Cp4.1LG00g08780 vs. TrEMBL
Match: A0A0A0LRB6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357340 PE=4 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 1.1e-31
Identity = 70/91 (76.92%), Postives = 77/91 (84.62%), Query Frame = 1 

Query: 1  MGNSKSKTYKRVLVPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEA 60
          MGNSKSKTYKRVLVP SEEQL ++FK+HD+DGDG L+KEELKQAF YLGSRF  FR EEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60

Query: 61 LRTVDDNHDGVVSMDEMNKLIQYTKGRKYTI 92
          LR  D N DGV+SMDEM KLI+Y K RKYTI
Sbjct: 61 LRAADTNGDGVISMDEMGKLIKYAKTRKYTI 91

[Close]
BLAST of Cp4.1LG00g08780 vs. TrEMBL
Match: A0A0A0LL93_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357850 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 2.1e-17
Identity = 47/71 (66.20%), Postives = 58/71 (81.69%), Query Frame = 1 

Query: 16 VSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVSMD 75
          +SEEQL  IFK HD D D KL+KEEL +AF+YLGSR++A R +EA+R VD NHDGV+S+D
Sbjct: 8  LSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLD 67

Query: 76 EMNKLIQYTKG 87
          EM+KLI Y KG
Sbjct: 68 EMSKLIVYVKG 78

[Close]
BLAST of Cp4.1LG00g08780 vs. TrEMBL
Match: A0A0A0LP43_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357350 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.4e-16
Identity = 43/86 (50.00%), Postives = 59/86 (68.60%), Query Frame = 1 

Query: 1  MGNSKSKTYKRVLVPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEA 60
          MG S    +K     ++E ++  +FK+HD+DGDG+L+KEELK AF YLG +F+ FR  +A
Sbjct: 1  MGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQA 60

Query: 61 LRTVDDNHDGVVSMDEMNKLIQYTKG 87
          L   DDN D V+S +EM+KLI YT G
Sbjct: 61 LHAADDNCDDVISFEEMSKLIMYTAG 86

[Close]
BLAST of Cp4.1LG00g08780 vs. TrEMBL
Match: A0A0A0L4V0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639740 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.7e-14
Identity = 42/83 (50.60%), Postives = 57/83 (68.67%), Query Frame = 1 

Query: 9  YKRVLVPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNH 68
          YKR  VP S +QL DIF  HD DGDG+LS+ ELK AF  LGS +S FRA ++L+  D + 
Sbjct: 14 YKRCPVPFSRDQLRDIFLKHDGDGDGRLSRLELKSAFESLGSNWSRFRARQSLKAADADG 73

Query: 69 DGVVSMDEMNKLIQYTKGRKYTI 92
          DG V++DE+++L+ Y    +Y I
Sbjct: 74 DGYVTLDELDRLLDYAVRCEYAI 96

[Close]
BLAST of Cp4.1LG00g08780 vs. TrEMBL
Match: M5XMC5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013983mg PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 9.1e-13
Identity = 40/79 (50.63%), Postives = 57/79 (72.15%), Query Frame = 1 

Query: 14 VPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVS 73
          VP ++EQ+ D+FK HD++GDG+LSKEEL  AF  LG+++  FRA  A +  DDN DG +S
Sbjct: 14 VPYAKEQIRDVFKRHDKNGDGQLSKEELDAAFDELGAKWPPFRAWFARQYADDNGDGFIS 73

Query: 74 MD-EMNKLIQYTKGRKYTI 92
          +D E++KL+QY    KY +
Sbjct: 74 IDKELSKLVQYALELKYIL 92

[Close]
BLAST of Cp4.1LG00g08780 vs. TAIR10
Match: AT1G18530.1 (AT1G18530.1 EF hand calcium-binding protein family)

HSP 1 Score: 51.2 bits (121), Expect = 3.9e-07
Identity = 26/68 (38.24%), Postives = 40/68 (58.82%), Query Frame = 1 

Query: 14  VPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVS 73
           V ++ EQLL+IFKS D+DG+G +S  EL  A + +G   +     E ++  D N DGV+S
Sbjct: 76  VLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEADTNGDGVIS 135

Query: 74  MDEMNKLI 82
             E   ++
Sbjct: 136 FGEFASIM 143

[Close]
BLAST of Cp4.1LG00g08780 vs. TAIR10
Match: AT3G25600.1 (AT3G25600.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 48.9 bits (115), Expect = 1.9e-06
Identity = 21/68 (30.88%), Postives = 42/68 (61.76%), Query Frame = 1 

Query: 14  VPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVS 73
           V +++EQL+++F+S D+DG+G ++  EL  + + +G   +     E +   D N DGV+S
Sbjct: 81  VLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEADSNGDGVIS 140

Query: 74  MDEMNKLI 82
            +E + ++
Sbjct: 141 FNEFSHIM 148

[Close]
BLAST of Cp4.1LG00g08780 vs. TAIR10
Match: AT3G51920.1 (AT3G51920.1 calmodulin 9)

HSP 1 Score: 47.4 bits (111), Expect = 5.6e-06
Identity = 21/65 (32.31%), Postives = 41/65 (63.08%), Query Frame = 1 

Query: 17  SEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVSMDE 76
           + ++L+++F+  D+DGDG +S+ EL +    +G + +A  AE  +R  D + DG +S  E
Sbjct: 82  ASDELIEVFRVFDRDGDGLISQLELGEGMKDMGMKITAEEAEHMVREADLDGDGFLSFHE 141

Query: 77  MNKLI 82
            +K++
Sbjct: 142 FSKMM 146

[Close]
BLAST of Cp4.1LG00g08780 vs. TAIR10
Match: AT3G50770.1 (AT3G50770.1 calmodulin-like 41)

HSP 1 Score: 47.0 bits (110), Expect = 7.4e-06
Identity = 23/60 (38.33%), Postives = 35/60 (58.33%), Query Frame = 1 

Query: 17  SEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVSMDE 76
           S+E+L  +F   D DGDGK+S  EL+  F  +G   S   A+EA+  VD + DG +  ++
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

[Close]
BLAST of Cp4.1LG00g08780 vs. TAIR10
Match: AT5G42380.1 (AT5G42380.1 calmodulin like 37)

HSP 1 Score: 46.6 bits (109), Expect = 9.6e-06
Identity = 24/67 (35.82%), Postives = 37/67 (55.22%), Query Frame = 1 

Query: 20  QLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVSMDEMNK 79
           +L  +F   D + DGK+S EEL+   S LG   S+   EE ++T D + DG +  +E  K
Sbjct: 49  ELRTVFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLK 108

Query: 80  LIQYTKG 87
           L++   G
Sbjct: 109 LMEGEDG 115

[Close]
BLAST of Cp4.1LG00g08780 vs. NCBI nr
Match: gi|700207392|gb|KGN62511.1| (hypothetical protein Csa_2G357340 [Cucumis sativus])

HSP 1 Score: 143.7 bits (361), Expect = 1.6e-31
Identity = 70/91 (76.92%), Postives = 77/91 (84.62%), Query Frame = 1 

Query: 1  MGNSKSKTYKRVLVPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEA 60
          MGNSKSKTYKRVLVP SEEQL ++FK+HD+DGDG L+KEELKQAF YLGSRF  FR EEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60

Query: 61 LRTVDDNHDGVVSMDEMNKLIQYTKGRKYTI 92
          LR  D N DGV+SMDEM KLI+Y K RKYTI
Sbjct: 61 LRAADTNGDGVISMDEMGKLIKYAKTRKYTI 91

[Close]
BLAST of Cp4.1LG00g08780 vs. NCBI nr
Match: gi|700207394|gb|KGN62513.1| (hypothetical protein Csa_2G357850 [Cucumis sativus])

HSP 1 Score: 96.3 bits (238), Expect = 3.0e-17
Identity = 47/71 (66.20%), Postives = 58/71 (81.69%), Query Frame = 1 

Query: 16 VSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVSMD 75
          +SEEQL  IFK HD D D KL+KEEL +AF+YLGSR++A R +EA+R VD NHDGV+S+D
Sbjct: 8  LSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLD 67

Query: 76 EMNKLIQYTKG 87
          EM+KLI Y KG
Sbjct: 68 EMSKLIVYVKG 78

[Close]
BLAST of Cp4.1LG00g08780 vs. NCBI nr
Match: gi|700207393|gb|KGN62512.1| (hypothetical protein Csa_2G357350 [Cucumis sativus])

HSP 1 Score: 93.6 bits (231), Expect = 1.9e-16
Identity = 43/86 (50.00%), Postives = 59/86 (68.60%), Query Frame = 1 

Query: 1  MGNSKSKTYKRVLVPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEA 60
          MG S    +K     ++E ++  +FK+HD+DGDG+L+KEELK AF YLG +F+ FR  +A
Sbjct: 1  MGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQA 60

Query: 61 LRTVDDNHDGVVSMDEMNKLIQYTKG 87
          L   DDN D V+S +EM+KLI YT G
Sbjct: 61 LHAADDNCDDVISFEEMSKLIMYTAG 86

[Close]
BLAST of Cp4.1LG00g08780 vs. NCBI nr
Match: gi|659087772|ref|XP_008444628.1| (PREDICTED: probable calcium-binding protein CML18 [Cucumis melo])

HSP 1 Score: 90.1 bits (222), Expect = 2.1e-15
Identity = 47/71 (66.20%), Postives = 55/71 (77.46%), Query Frame = 1 

Query: 16  VSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVSMD 75
           +SEEQL  IFK HD D D KL+KEEL +AF YLGSR++A R + ALR VD N DGV+SMD
Sbjct: 42  LSEEQLAWIFKKHDIDRDRKLTKEELARAFDYLGSRWTARRVKGALRAVDANGDGVISMD 101

Query: 76  EMNKLIQYTKG 87
           EM+KLI Y KG
Sbjct: 102 EMSKLIVYVKG 112

[Close]
BLAST of Cp4.1LG00g08780 vs. NCBI nr
Match: gi|700200028|gb|KGN55186.1| (hypothetical protein Csa_4G639740 [Cucumis sativus])

HSP 1 Score: 86.7 bits (213), Expect = 2.4e-14
Identity = 42/83 (50.60%), Postives = 57/83 (68.67%), Query Frame = 1 

Query: 9  YKRVLVPVSEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNH 68
          YKR  VP S +QL DIF  HD DGDG+LS+ ELK AF  LGS +S FRA ++L+  D + 
Sbjct: 14 YKRCPVPFSRDQLRDIFLKHDGDGDGRLSRLELKSAFESLGSNWSRFRARQSLKAADADG 73

Query: 69 DGVVSMDEMNKLIQYTKGRKYTI 92
          DG V++DE+++L+ Y    +Y I
Sbjct: 74 DGYVTLDELDRLLDYAVRCEYAI 96

The following BLAST results are available for this feature:
BLAST of Cp4.1LG00g08780 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML15_ARATH6.9e-0638.24Probable calcium-binding protein CML15 OS=Arabidopsis thaliana GN=CML15 PE=2 SV=... [more]
BLAST of Cp4.1LG00g08780 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LRB6_CUCSA1.1e-3176.92Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357340 PE=4 SV=1[more]
A0A0A0LL93_CUCSA2.1e-1766.20Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357850 PE=4 SV=1[more]
A0A0A0LP43_CUCSA1.4e-1650.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357350 PE=4 SV=1[more]
A0A0A0L4V0_CUCSA1.7e-1450.60Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639740 PE=4 SV=1[more]
M5XMC5_PRUPE9.1e-1350.63Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013983mg PE=4 SV=1[more]
BLAST of Cp4.1LG00g08780 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18530.13.9e-0738.24 EF hand calcium-binding protein family[more]
AT3G25600.11.9e-0630.88 Calcium-binding EF-hand family protein[more]
AT3G51920.15.6e-0632.31 calmodulin 9[more]
AT3G50770.17.4e-0638.33 calmodulin-like 41[more]
AT5G42380.19.6e-0635.82 calmodulin like 37[more]
BLAST of Cp4.1LG00g08780 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700207392|gb|KGN62511.1|1.6e-3176.92hypothetical protein Csa_2G357340 [Cucumis sativus][more]
gi|700207394|gb|KGN62513.1|3.0e-1766.20hypothetical protein Csa_2G357850 [Cucumis sativus][more]
gi|700207393|gb|KGN62512.1|1.9e-1650.00hypothetical protein Csa_2G357350 [Cucumis sativus][more]
gi|659087772|ref|XP_008444628.1|2.1e-1566.20PREDICTED: probable calcium-binding protein CML18 [Cucumis melo][more]
gi|700200028|gb|KGN55186.1|2.4e-1450.60hypothetical protein Csa_4G639740 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG00g08780.1Cp4.1LG00g08780.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 18..81
score: 1.9
IPR002048EF-hand domainSMARTSM00054efh_1coord: 56..84
score: 3.2coord: 20..48
score: 0
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 52..87
score: 9.855coord: 16..51
score: 15
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 15..82
score: 9.8
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 7..81
score: 7.54
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 65..77
score: -coord: 29..41
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 13..86
score: 6.6
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 13..86
score: 6.6

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG00g08780Cucurbita pepo (Zucchini)cpecpeB001