Cp4.1LG00g03620 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG00g03620
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionC-4 sterol methyl oxidase 2
LocationCp4.1LG00 : 13740090 .. 13744583 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGCAGTATCTGATCACAAACTTCAGTGATTTTCAACTAGCTTGTATTGGAAGCTTTATTATCCATGAAAGCGTGTTCTTCTTATCTGGGCTTCCCTTTGTTTTTCTGGAAAGAGCAGGGTGGCTAAGCAAGTACAAGATCCAGGTTTGCCCTCTTGCTTCACTAGAAATCTTCATAAGTTCTTATGGATGCTTGATCAATATTTGATATTTGAATAAGATTTACCTTTGTTTGTGATTAATGTATGATTATGAACTAGATTAGATATAATTAGTGCTTTAAGCTCTATTTCTCATTGATTATTCTTTCTATCAAGGAGAATGATATGATATGAAAAGAAAAGAAAATCACTGCCTCTTGAGCTGATACTTATGGATGCAGGCAAAGAACCTATCAACTGTGTTTTATCGCTCGAGTAATTAACTCGACTAATTATGGGTTAATTTTTTCTGCTTTATTCAGGCTAAGAATAATAGTCCTGCTGCTCAAGGAAAATGTATTTCGCGCCTACTGCTCTATCACTTTGGTGTAAATCTGCCCGTTATGATCGTTTCGTATCCGGTGTTCAAGCGTATGGGCATGAGAAGCACTCTTCCATTGCCATCCTGGTATCTTCAGGTTCTTTATGTTCTAAAGCATATATTTTAGGAGTTTTATCTAGCTAGGTTTAGTTTTTACGTTTTTTTGTGCAATCTCATATTGATTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTACTAGACGCGTTTTAAAACCTTGAGGGGAAGCCTGAAAGGAAAAGTTCAAAGAGGGCAATATCTGCTAGCGGTGGGCTTGGGCCGTTACGACCCCACATCGGTTGGGGAGGAGAACGAAACCTTCTTTATAAGGGTGTGGAAACGTCTCCCTATTAGACGCGTTTTAAAACATTGAGGAGAAGCCCAAAAGCGAAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGGCCGTTACAATCTCACATCGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTACTAGACGCGTTTTAAAACCTTGAGGGGAAGCCTGAAAGGAAAAGTTCAAAGAGGGCAATATCTGCTAGCGGTGGGCTTGGGCCGTTACGACCCCACATCGGTTGGGGAGGAGAACGAAACCTTCTTTATAAGGGTGTGGAAACGTCTCCCTATTAGACGCGTTTTAAAACATTGAGGAGAAGCCCAAAAGCGAAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGGCCGTTACAATCTCACATCGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGATGCGTTTTAAAACCTTGTGGGGAAGCCGGGAAGGGAAAGCCCCAAAAGGACAATATCTACTAGCGGTGGGCTTGGGTCGTTACAATCCCACATTGGGTGGGGAGGAGAATGAAACATTCTTTATAAGGGTATAGAAACCTCTCTCTAGTAGGCGCGTTTTAAAACAAAGAGGACAATATCTGCTAACGGTGGGCTTGGATCGTTACAACCCCACATCGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAACCTTGAGGGGAAGTTCAAAATGGGAAGCCCAAAGAAGACAATATCTGCTAGTGGTTGGCTTGGGCTGTTACAACCCCACATCAGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTTCCTAGACGCGTTTTAAAACCTTGAAGGGAAGCTTGGAAGGCAAAGCCCAAAGAGGACAATAAGTGATAAGATAAGGAACCAACTACAAGGGGAATAAGATTCGGATTACCTTGTTGATCGAATATCTCAAGGCAAGAACACTTGATTGAGATTCGAATCACTCCACAAGCAAGATTGATCATGTCGAGCTTGAATGATTCTACATGCAATCTAAACTACATAGAATTGCAAAGAAACTTAGCCATTGGCTAAAGAAAAGCACAAATGCTTCTTTCTATATTTTCCAAGTCTACCTTACAAATACAACATACATGGCTTTATATAGCCTCAAAATGAAACTATTAAAGGCATTCCAAAAGTTGTAACATTCATACTTTATGGCCATAATTAGCCATTATGTAAATGTAACCTAAAGTAAATAAAATGTCTTAAAATACAATAACTCTAAATTACTCTAAATTGTAACCCACCCAAAATTTATAACCATCAAACTTCATTCCTCTTCAATGTGACATGAATTGCAACCTCTTTTGATAATTTTGACAACATTTTCTTCACATCTTCATTGAAGTATATTGTATGAATGATGTCTCTTGGTTCATATCAGGTGGGCTTGGACCGTTACATTTTCCTTATCTTGGGTTATATCATTACCTTTTCAAGAATTTAATTTGGTTTGCGTTTCATTCATGTAGGAAAGTCGTTTTCGGCCAGATAATATTTTACTTTATTGTGGAGGATTTTGTTTTCTACTGGGGCCATAGAATTTTACACACCAAATGGCTGTACAAGAACGTCCACAGCGTGCATCACGAGTAAGCGAGATTTACGAATGAAAATCCATGGATATGCTGCCTTCTTCCATGATAAAATCGTCTTAGGCTGTTTTATCGTTCTTATACGTGCAGATACGCTACCCCTTTTGGACTAACATCAGAATATGCTCACCCTGCCGAGATCCTGTTCCTCGGATTCGCAACCATTGTCGGTCCTGCTCTTACCGGTCCCCATCTAATGACTCTGTGGTTATGGATGGTACTTCGAGTGCTAGAGACGGTCGAGGCTCATTGTGGTTACGATTTTCCTTGGAGCCCTTCAAACTTCATACCTTTGTATGGGGGGTGAGTTGATTTGGCTTTGTAATGTCTTAAAGGAATACTTTGCTCTTTATCTGTTGTTCCTGAAATCAGCTTTGCTCTTTCTCCACAGTGCTTACTTTCATGATTATCATCACCGGCTGCTTTATACAAAATCTGGCAACTACTCGTCGACGTTCACTTACATGGACTGGTAAGTTCTGGTCCGAACAAGGTCGAATCGTTTATTACGTTATTGGTTTGAGCTTCATAGCATTAAAACCCGCTTTGTGGAACATTGATTTACCATTCATTCAATAGTTTCACGACTATTTCCTCGACTGTTGATCTGTGTAGTAAGTGTCGGTAAGCAAGGGGGTTGTTTGAATAATAGCTAGATGATGATCGTTGAATTACGAATGCACACCACATTCTTCAGTCGCTTTTTCTCTTGTCCTATGATACTATTGATGACCAATCAAACTCTGGAGAAGAAGCAAGCTTCAACGCTCACCAAGAGTACAGAGCCCTCATTATGGGGAGGGTAAAGAGCTTCCAACGACCTTTCATTCCCTTGTTTCGTCGTGGCACACCCCACACACAAACACCACCCGGCTTAGGGGGACTAGAAAACACCCTTCGGTTTACCCCCAGCCCTTGCCGCCTTCCATAACAAGCCCTTGAGCTTCCTTTGCGCTACCGTCCTCCTAGAAGCCACCAGGTTGACCCCTAGGCTTTTTAGGAATTACCTGAAACTTGAGCATCTTGTATTTCATCAAAATTGGGGATCCCCTTAAACATTCTTTTTTAGACAAAGGGCTTGACTTTTATCGGGTTAGCGTCGAGAAAGAGTACACCAACTAGTTGGCTATGTAAAAAGAGAGTGGGCTACCGAGAACACGAAGCTTGCTCTCTATTCGATCTTTCATCGAGCAGTAAACACGAAAAGGAGTTTTCATCTAGCTTGCATCTGTCATTTACCAAATTTGATCTCTTTGTACATGAAATATCTTGTAGAGATCTATATAGGTTTTGAACATCTGAAGAGACTCATCATCTGAATGGCTAACATGGAACTTTCATATGGGAAAGTCATATTTATATGCATTTCACGTTTTTGCATGTTCATGTTCATGGATTATCAATCATTTCTATGCATGGAAATAGCAGAAAAACACCGTCGATAACGTTTCGACTTCTTTGATCGGCAGGATATTTGGAACCGATAAGGGTTTCAGAAATTTGGAAGCTATAAAAAAGGCGGAAAGTTGATGAGACGAAAGAGAACGAGCTGAGGAAAACGTCGGGTACGTTCGAGATAGGTTTCTGATGTATAGGCCGACTCGAAAAGGTTTAGTTCTTCTATATCATTACCCTTTCTTGTGTGCCATGATGTGTTTTGTGTCTGTTTTGTTGCTTAAAATGGCTGTATTCCGAAGCTAATGTTGAGCTTCTACTATGGAATTTGGCTGTCATCTTCTTGTGGTTCAGTTAAAACTCTGTGTTCTTCTACCAATTGGCTTTCCATGTGACTTTATCTGTGTTGTGAAAAAGGTTTGAAGCTCATGTGTTAATGTCAAAAAGGAAAACTTAGAAGTTTATGGCATTGAGGAAATCGGGTCTTTAAAAT

mRNA sequence

ATGGTGCAGTATCTGATCACAAACTTCAGTGATTTTCAACTAGCTTGTATTGGAAGCTTTATTATCCATGAAAGCGTGTTCTTCTTATCTGGGCTTCCCTTTGTTTTTCTGGAAAGAGCAGGGTGGCTAAGCAAGTACAAGATCCAGGCTAAGAATAATAGTCCTGCTGCTCAAGGAAAATGTATTTCGCGCCTACTGCTCTATCACTTTGGTGTAAATCTGCCCGTTATGATCGTTTCGTATCCGGTGTTCAAGCGTATGGGCATGAGAAGCACTCTTCCATTGCCATCCTGGAAAGTCGTTTTCGGCCAGATAATATTTTACTTTATTGTGGAGGATTTTGTTTTCTACTGGGGCCATAGAATTTTACACACCAAATGGCTGTACAAGAACGTCCACAGCGTGCATCACGAATACGCTACCCCTTTTGGACTAACATCAGAATATGCTCACCCTGCCGAGATCCTGTTCCTCGGATTCGCAACCATTGTCGGTCCTGCTCTTACCGGTCCCCATCTAATGACTCTGTGGTTATGGATGGTACTTCGAGTGCTAGAGACGGTCGAGGCTCATTGTGGTTACGATTTTCCTTGGAGCCCTTCAAACTTCATACCTTTGTATGGGGGTGCTTACTTTCATGATTATCATCACCGGCTGCTTTATACAAAATCTGGCAACTACTCGTCGACGTTCACTTACATGGACTGGATATTTGGAACCGATAAGGGTTTCAGAAATTTGGAAGCTATAAAAAAGGCGGAAAGTTGATGAGACGAAAGAGAACGAGCTGAGGAAAACGTCGGGTACGTTCGAGAAGGTTTAGTTCTTCTATATCATTACCCTTTCTTGTGTGCCATGATGTGTTTTGTGTCTGTTTTGTTGCTTAAAATGGCTGTATTCCGAAGCTAATGTTGAGCTTCTACTATGGAATTTGGCTGTCATCTTCTTGTGGTTCAGTTAAAACTCTGTGTTCTTCTACCAATTGGCTTTCCATGTGACTTTATCTGTGTTGTGAAAAAGGTTTGAAGCTCATGTGTTAATGTCAAAAAGGAAAACTTAGAAGTTTATGGCATTGAGGAAATCGGGTCTTTAAAAT

Coding sequence (CDS)

ATGGTGCAGTATCTGATCACAAACTTCAGTGATTTTCAACTAGCTTGTATTGGAAGCTTTATTATCCATGAAAGCGTGTTCTTCTTATCTGGGCTTCCCTTTGTTTTTCTGGAAAGAGCAGGGTGGCTAAGCAAGTACAAGATCCAGGCTAAGAATAATAGTCCTGCTGCTCAAGGAAAATGTATTTCGCGCCTACTGCTCTATCACTTTGGTGTAAATCTGCCCGTTATGATCGTTTCGTATCCGGTGTTCAAGCGTATGGGCATGAGAAGCACTCTTCCATTGCCATCCTGGAAAGTCGTTTTCGGCCAGATAATATTTTACTTTATTGTGGAGGATTTTGTTTTCTACTGGGGCCATAGAATTTTACACACCAAATGGCTGTACAAGAACGTCCACAGCGTGCATCACGAATACGCTACCCCTTTTGGACTAACATCAGAATATGCTCACCCTGCCGAGATCCTGTTCCTCGGATTCGCAACCATTGTCGGTCCTGCTCTTACCGGTCCCCATCTAATGACTCTGTGGTTATGGATGGTACTTCGAGTGCTAGAGACGGTCGAGGCTCATTGTGGTTACGATTTTCCTTGGAGCCCTTCAAACTTCATACCTTTGTATGGGGGTGCTTACTTTCATGATTATCATCACCGGCTGCTTTATACAAAATCTGGCAACTACTCGTCGACGTTCACTTACATGGACTGGATATTTGGAACCGATAAGGGTTTCAGAAATTTGGAAGCTATAAAAAAGGCGGAAAGTTGA

Protein sequence

MVQYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCISRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLRVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKGFRNLEAIKKAES
BLAST of Cp4.1LG00g03620 vs. Swiss-Prot
Match: SMO22_ARATH (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 3.0e-131
Identity = 214/250 (85.60%), Postives = 235/250 (94.00%), Query Frame = 1

Query: 3   QYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCI 62
           QYL+T+FSDFQLACIGSF++HESVFFLSGLPF+FLER G+LSKYKIQ KNN+PAAQGKCI
Sbjct: 10  QYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNNTPAAQGKCI 69

Query: 63  SRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRI 122
           +RLLLYHF VNLP+M+ SYPVF+ MGMRS+ PLPSWK V  QI+FYFI+EDFVFYWGHRI
Sbjct: 70  TRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRI 129

Query: 123 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVL 182
           LH+KWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL+TLWLWMVL
Sbjct: 130 LHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVL 189

Query: 183 RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 242
           RVLETVEAHCGY FPWS SNF+PLYGGA FHDYHHRLLYTKSGNYSSTF YMDWIFGTDK
Sbjct: 190 RVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDK 249

Query: 243 GFRNLEAIKK 253
           G+R L+ +K+
Sbjct: 250 GYRRLKTLKE 259

BLAST of Cp4.1LG00g03620 vs. Swiss-Prot
Match: SMO21_ARATH (Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana GN=SMO2-1 PE=2 SV=2)

HSP 1 Score: 453.0 bits (1164), Expect = 2.2e-126
Identity = 210/262 (80.15%), Postives = 234/262 (89.31%), Query Frame = 1

Query: 3   QYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCI 62
           +YL+T+FSDFQLACIGSFI+HESVFFLSGLP++FLER G+LS YKIQ K+N+P AQGKCI
Sbjct: 10  KYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPEAQGKCI 69

Query: 63  SRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRI 122
           +RLLLYH  VNLP+M+ SYPVF+ MGM S+ PLPSWKVV  QI+FYFI+EDFVFYWGHRI
Sbjct: 70  ARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRI 129

Query: 123 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVL 182
           LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL+TLWLWM+L
Sbjct: 130 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMML 189

Query: 183 RVLETVEAHCGYDFPWSPSNFIPLYGG------------AYFHDYHHRLLYTKSGNYSST 242
           RV+ETVEAHCGY FPWSPSNF+PLYGG            A FHDYHHRLLYTKSGNYSST
Sbjct: 190 RVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLYTKSGNYSST 249

Query: 243 FTYMDWIFGTDKGFRNLEAIKK 253
           F YMDWIFGTDKG+R L+A+K+
Sbjct: 250 FVYMDWIFGTDKGYRKLKALKE 271

BLAST of Cp4.1LG00g03620 vs. Swiss-Prot
Match: MSMOB_DICDI (Putative methylsterol monooxygenase DDB_G0269788 OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 7.9e-60
Identity = 111/242 (45.87%), Postives = 154/242 (63.64%), Query Frame = 1

Query: 14  LACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQ-AKNNSPAAQGKCISRLLLYHFGV 73
           L   G+FI HE  +F S +PF   +   +L KYKIQ  K N    Q  CI ++L+ H  V
Sbjct: 25  LITYGTFIAHEVFYFGSFIPFFLCDFMPFLQKYKIQPTKKNEWKTQFNCIFKVLMTHIFV 84

Query: 74  NLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRILHTKWLYKNV 133
            LP+M +  P  K +G+ +  PLPS   +   I   F++EDF FYW HR LH  + YK++
Sbjct: 85  QLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACCFLIEDFYFYWVHRALHHGFWYKHI 144

Query: 134 HSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLRVLETVEAHC 193
           H VHH++A PFG+T+EYAHP E + LG  T++GP L    L TLW+W+  R+ +TVE H 
Sbjct: 145 HKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFLFSRDLFTLWVWLGTRLFQTVECHS 204

Query: 194 GYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKGFRNLEAIKK 253
           GYDFPW+P+  IP +GG++FHD+HH    T  GNYSSTFTY+D IFGT   + + + I+ 
Sbjct: 205 GYDFPWNPTKLIPFWGGSHFHDFHHE---TFVGNYSSTFTYLDKIFGTSDKYYSRKQIRD 263

Query: 254 AE 255
           ++
Sbjct: 265 SK 263

BLAST of Cp4.1LG00g03620 vs. Swiss-Prot
Match: SMO11_ARATH (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 8.8e-59
Identity = 114/253 (45.06%), Postives = 156/253 (61.66%), Query Frame = 1

Query: 10  SDFQLACIGSFIIHESVFFLSGLPFVFLERA----GWLSKYKIQAK-NNSPAAQGKCISR 69
           SD+ L C     +   VF L  LP VF+E A    G  ++YKIQ K N S +   KC   
Sbjct: 33  SDYYLYCHNILFLF-LVFSLVPLPLVFVELARSASGLFNRYKIQPKVNYSLSDMFKCYKD 92

Query: 70  LLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRILH 129
           ++     V  P+ +VSYP  + + +RS LPLP+   +  Q++ YF++ED+  YW HR  H
Sbjct: 93  VMTMFILVVGPLQLVSYPSIQMIEIRSGLPLPTITEMLSQLVVYFLIEDYTNYWVHRFFH 152

Query: 130 TKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLRV 189
           +KW Y  +H VHHEY  P G  + YAH AE+L LG  T +GPA+   H++T WLW+ LR 
Sbjct: 153 SKWGYDKIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAIAPGHMITFWLWIALRQ 212

Query: 190 LETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKGF 249
           +E +E H GYDFPWSP+ +IP YGGA +HDYHH +      N++S FTY D+I+GTDKG+
Sbjct: 213 MEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGY 272

Query: 250 R----NLEAIKKA 254
           R     LE IK++
Sbjct: 273 RFQKKLLEQIKES 284

BLAST of Cp4.1LG00g03620 vs. Swiss-Prot
Match: SMO13_ARATH (Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana GN=SMO1-3 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.3e-57
Identity = 106/237 (44.73%), Postives = 147/237 (62.03%), Query Frame = 1

Query: 10  SDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAK-NNSPAAQGKCISRLLLY 69
           S+F + C  + ++   VF L+  P V +E  GW  ++KIQ K   S +   +C   ++  
Sbjct: 33  SNFHVYC-HTILVLFLVFSLAPFPLVIVEWTGWFDQFKIQKKVKYSLSDMFQCYKEVMKL 92

Query: 70  HFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRILHTKWL 129
              V   + IVSYP  + +G+RS LPLPS   +  Q++ YF++ED+  YW HR +H KW 
Sbjct: 93  FLLVVGTLQIVSYPSIQMVGIRSGLPLPSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWG 152

Query: 130 YKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLRVLETV 189
           Y+ +H +HHEY +P G  S YAH AEIL LG  T +GPA+   H+MT WLW+ LR  E +
Sbjct: 153 YEKIHRIHHEYTSPIGYASPYAHWAEILILGIPTFLGPAIAPGHIMTFWLWISLRQFEAI 212

Query: 190 EAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKGFR 246
           E H GYDFPWS +  IP YGG  +HDYHH +      N++S FTY D+I+GTDKG+R
Sbjct: 213 ETHSGYDFPWSVTKLIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYR 268

BLAST of Cp4.1LG00g03620 vs. TrEMBL
Match: A0A0A0LVE5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G524750 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 2.3e-146
Identity = 245/253 (96.84%), Postives = 251/253 (99.21%), Query Frame = 1

Query: 3   QYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCI 62
           QYLITNFSDFQLACIGSFIIHESVFFLSGLPF+ LERAGWLSKYKIQAKNNSPAAQGKCI
Sbjct: 10  QYLITNFSDFQLACIGSFIIHESVFFLSGLPFILLERAGWLSKYKIQAKNNSPAAQGKCI 69

Query: 63  SRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRI 122
           SRLLLYHFGVNLPVM+VSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFI+EDFVFYWGHRI
Sbjct: 70  SRLLLYHFGVNLPVMLVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIIEDFVFYWGHRI 129

Query: 123 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVL 182
           LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATI+GPALTGPHL TLWLWMV+
Sbjct: 130 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIIGPALTGPHLFTLWLWMVV 189

Query: 183 RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 242
           RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK
Sbjct: 190 RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 249

Query: 243 GFRNLEAIKKAES 256
           GFRNLEAIKKAE+
Sbjct: 250 GFRNLEAIKKAEN 262

BLAST of Cp4.1LG00g03620 vs. TrEMBL
Match: V4SCC9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029052mg PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 1.1e-132
Identity = 222/251 (88.45%), Postives = 238/251 (94.82%), Query Frame = 1

Query: 1   MVQYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGK 60
           M QYLIT+FSDFQLAC+GSF +HES+FFL+GLPF++LERAGWLSKYKIQ KNNSPAAQ K
Sbjct: 1   MTQYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKNNSPAAQEK 60

Query: 61  CISRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGH 120
           CI+RLLLYHFGVNLPVMI SYPVFK MGM+S+LPLPSWKVV  QIIFYFI+EDFVFYWGH
Sbjct: 61  CITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILEDFVFYWGH 120

Query: 121 RILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWM 180
           RILHTKWLYK+VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHLMTLWLWM
Sbjct: 121 RILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWM 180

Query: 181 VLRVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGT 240
           VLRVLETVEAHCGY FPWS SNFIPLYGGA FHDYHHRLLYTKSGNYSSTF YMDWIFGT
Sbjct: 181 VLRVLETVEAHCGYHFPWSLSNFIPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 240

Query: 241 DKGFRNLEAIK 252
           DKG+R L+A+K
Sbjct: 241 DKGYRKLKALK 251

BLAST of Cp4.1LG00g03620 vs. TrEMBL
Match: A0A067FH15_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g024309mg PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 9.2e-132
Identity = 220/249 (88.35%), Postives = 237/249 (95.18%), Query Frame = 1

Query: 3   QYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCI 62
           +YLIT+FSDFQLAC+GSF +HES+FFL+GLPF++LERAGWLSKYKIQ KNNSPAAQ KCI
Sbjct: 10  RYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKNNSPAAQEKCI 69

Query: 63  SRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRI 122
           +RLLLYHFGVNLPVMI SYPVFK MGM+S+LPLPSWKVV  QIIFYFI+EDFVFYWGHRI
Sbjct: 70  TRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILEDFVFYWGHRI 129

Query: 123 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVL 182
           LHTKWLYK+VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHLMTLWLWMVL
Sbjct: 130 LHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVL 189

Query: 183 RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 242
           RVLETVEAHCGY FPWS SNFIPLYGGA FHDYHHRLLYTKSGNYSSTF YMDWIFGTDK
Sbjct: 190 RVLETVEAHCGYHFPWSLSNFIPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDK 249

Query: 243 GFRNLEAIK 252
           G+R L+A+K
Sbjct: 250 GYRKLKALK 258

BLAST of Cp4.1LG00g03620 vs. TrEMBL
Match: V4UFS8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029052mg PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 9.2e-132
Identity = 220/249 (88.35%), Postives = 237/249 (95.18%), Query Frame = 1

Query: 3   QYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCI 62
           +YLIT+FSDFQLAC+GSF +HES+FFL+GLPF++LERAGWLSKYKIQ KNNSPAAQ KCI
Sbjct: 10  RYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKNNSPAAQEKCI 69

Query: 63  SRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRI 122
           +RLLLYHFGVNLPVMI SYPVFK MGM+S+LPLPSWKVV  QIIFYFI+EDFVFYWGHRI
Sbjct: 70  TRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILEDFVFYWGHRI 129

Query: 123 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVL 182
           LHTKWLYK+VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHLMTLWLWMVL
Sbjct: 130 LHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVL 189

Query: 183 RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 242
           RVLETVEAHCGY FPWS SNFIPLYGGA FHDYHHRLLYTKSGNYSSTF YMDWIFGTDK
Sbjct: 190 RVLETVEAHCGYHFPWSLSNFIPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDK 249

Query: 243 GFRNLEAIK 252
           G+R L+A+K
Sbjct: 250 GYRKLKALK 258

BLAST of Cp4.1LG00g03620 vs. TrEMBL
Match: A0A067L4U4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02933 PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 2.3e-130
Identity = 218/248 (87.90%), Postives = 235/248 (94.76%), Query Frame = 1

Query: 4   YLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCIS 63
           YLIT+FSDFQLAC+GSF +HESVFFLSGLPF++LERAGWLSKYKIQ KNNSPAAQ KCI+
Sbjct: 11  YLITHFSDFQLACLGSFFLHESVFFLSGLPFIYLERAGWLSKYKIQTKNNSPAAQEKCIT 70

Query: 64  RLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRIL 123
           RLLLYHFGVNLPVM+ SYPVF+ MGMRS LPLPSWKVV  QIIFYFI+EDFVFYWGHRIL
Sbjct: 71  RLLLYHFGVNLPVMLFSYPVFRYMGMRSDLPLPSWKVVLTQIIFYFILEDFVFYWGHRIL 130

Query: 124 HTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLR 183
           HTKWLYK+VHSVHHEYATPFGLTSEYAHPAEILFLGFATI+GPA+TGPHL+TLWLWM+LR
Sbjct: 131 HTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIIGPAITGPHLITLWLWMMLR 190

Query: 184 VLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKG 243
           VLETVEAHCGY FPWS SNFIPLYGGA FHDYHHRLLYTKSGNYSSTF YMDWIFGTDKG
Sbjct: 191 VLETVEAHCGYHFPWSLSNFIPLYGGADFHDYHHRLLYTKSGNYSSTFIYMDWIFGTDKG 250

Query: 244 FRNLEAIK 252
           +R L+A+K
Sbjct: 251 YRKLKALK 258

BLAST of Cp4.1LG00g03620 vs. TAIR10
Match: AT1G07420.1 (AT1G07420.1 sterol 4-alpha-methyl-oxidase 2-1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.7e-132
Identity = 214/250 (85.60%), Postives = 235/250 (94.00%), Query Frame = 1

Query: 3   QYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCI 62
           QYL+T+FSDFQLACIGSF++HESVFFLSGLPF+FLER G+LSKYKIQ KNN+PAAQGKCI
Sbjct: 10  QYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNNTPAAQGKCI 69

Query: 63  SRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRI 122
           +RLLLYHF VNLP+M+ SYPVF+ MGMRS+ PLPSWK V  QI+FYFI+EDFVFYWGHRI
Sbjct: 70  TRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRI 129

Query: 123 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVL 182
           LH+KWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL+TLWLWMVL
Sbjct: 130 LHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVL 189

Query: 183 RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 242
           RVLETVEAHCGY FPWS SNF+PLYGGA FHDYHHRLLYTKSGNYSSTF YMDWIFGTDK
Sbjct: 190 RVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDK 249

Query: 243 GFRNLEAIKK 253
           G+R L+ +K+
Sbjct: 250 GYRRLKTLKE 259

BLAST of Cp4.1LG00g03620 vs. TAIR10
Match: AT2G29390.1 (AT2G29390.1 sterol 4-alpha-methyl-oxidase 2-2)

HSP 1 Score: 453.0 bits (1164), Expect = 1.2e-127
Identity = 210/262 (80.15%), Postives = 234/262 (89.31%), Query Frame = 1

Query: 3   QYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCI 62
           +YL+T+FSDFQLACIGSFI+HESVFFLSGLP++FLER G+LS YKIQ K+N+P AQGKCI
Sbjct: 10  KYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPEAQGKCI 69

Query: 63  SRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRI 122
           +RLLLYH  VNLP+M+ SYPVF+ MGM S+ PLPSWKVV  QI+FYFI+EDFVFYWGHRI
Sbjct: 70  ARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRI 129

Query: 123 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVL 182
           LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL+TLWLWM+L
Sbjct: 130 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMML 189

Query: 183 RVLETVEAHCGYDFPWSPSNFIPLYGG------------AYFHDYHHRLLYTKSGNYSST 242
           RV+ETVEAHCGY FPWSPSNF+PLYGG            A FHDYHHRLLYTKSGNYSST
Sbjct: 190 RVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLYTKSGNYSST 249

Query: 243 FTYMDWIFGTDKGFRNLEAIKK 253
           F YMDWIFGTDKG+R L+A+K+
Sbjct: 250 FVYMDWIFGTDKGYRKLKALKE 271

BLAST of Cp4.1LG00g03620 vs. TAIR10
Match: AT4G12110.1 (AT4G12110.1 sterol-4alpha-methyl oxidase 1-1)

HSP 1 Score: 228.4 bits (581), Expect = 4.9e-60
Identity = 114/253 (45.06%), Postives = 156/253 (61.66%), Query Frame = 1

Query: 10  SDFQLACIGSFIIHESVFFLSGLPFVFLERA----GWLSKYKIQAK-NNSPAAQGKCISR 69
           SD+ L C     +   VF L  LP VF+E A    G  ++YKIQ K N S +   KC   
Sbjct: 33  SDYYLYCHNILFLF-LVFSLVPLPLVFVELARSASGLFNRYKIQPKVNYSLSDMFKCYKD 92

Query: 70  LLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRILH 129
           ++     V  P+ +VSYP  + + +RS LPLP+   +  Q++ YF++ED+  YW HR  H
Sbjct: 93  VMTMFILVVGPLQLVSYPSIQMIEIRSGLPLPTITEMLSQLVVYFLIEDYTNYWVHRFFH 152

Query: 130 TKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLRV 189
           +KW Y  +H VHHEY  P G  + YAH AE+L LG  T +GPA+   H++T WLW+ LR 
Sbjct: 153 SKWGYDKIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAIAPGHMITFWLWIALRQ 212

Query: 190 LETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKGF 249
           +E +E H GYDFPWSP+ +IP YGGA +HDYHH +      N++S FTY D+I+GTDKG+
Sbjct: 213 MEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGY 272

Query: 250 R----NLEAIKKA 254
           R     LE IK++
Sbjct: 273 RFQKKLLEQIKES 284

BLAST of Cp4.1LG00g03620 vs. TAIR10
Match: AT4G22753.1 (AT4G22753.1 sterol 4-alpha methyl oxidase 1-3)

HSP 1 Score: 224.6 bits (571), Expect = 7.1e-59
Identity = 106/237 (44.73%), Postives = 147/237 (62.03%), Query Frame = 1

Query: 10  SDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAK-NNSPAAQGKCISRLLLY 69
           S+F + C  + ++   VF L+  P V +E  GW  ++KIQ K   S +   +C   ++  
Sbjct: 33  SNFHVYC-HTILVLFLVFSLAPFPLVIVEWTGWFDQFKIQKKVKYSLSDMFQCYKEVMKL 92

Query: 70  HFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRILHTKWL 129
              V   + IVSYP  + +G+RS LPLPS   +  Q++ YF++ED+  YW HR +H KW 
Sbjct: 93  FLLVVGTLQIVSYPSIQMVGIRSGLPLPSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWG 152

Query: 130 YKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLRVLETV 189
           Y+ +H +HHEY +P G  S YAH AEIL LG  T +GPA+   H+MT WLW+ LR  E +
Sbjct: 153 YEKIHRIHHEYTSPIGYASPYAHWAEILILGIPTFLGPAIAPGHIMTFWLWISLRQFEAI 212

Query: 190 EAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKGFR 246
           E H GYDFPWS +  IP YGG  +HDYHH +      N++S FTY D+I+GTDKG+R
Sbjct: 213 ETHSGYDFPWSVTKLIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYR 268

BLAST of Cp4.1LG00g03620 vs. TAIR10
Match: AT4G22756.1 (AT4G22756.1 sterol C4-methyl oxidase 1-2)

HSP 1 Score: 221.5 bits (563), Expect = 6.0e-58
Identity = 108/241 (44.81%), Postives = 148/241 (61.41%), Query Frame = 1

Query: 10  SDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLS----KYKIQAK-NNSPAAQGKCISR 69
           SD+ L C     +   +F L  LP VF+E +   S    +YKIQ K  NS ++  KC   
Sbjct: 33  SDYYLYCHNILFLF-LIFSLVPLPLVFIESSQSTSDLFNRYKIQPKVKNSFSSMFKCYKD 92

Query: 70  LLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRILH 129
           ++     V  P+ +VSYP  + + +RS LPLPS   +  Q++ YF+VED+  YW HR  H
Sbjct: 93  VMKMFILVVGPLQLVSYPSIQMIEIRSGLPLPSCMEIVAQLVVYFLVEDYTNYWVHRFFH 152

Query: 130 TKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLRV 189
            KW Y+  H +HHEY  P G  + YAH AE+L LG  T +GPA+   H++T WLW+ LR 
Sbjct: 153 CKWGYEKFHHIHHEYTAPIGYAAPYAHWAEVLLLGIPTFLGPAIAPGHMITFWLWIALRQ 212

Query: 190 LETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKGF 246
           +E +E H GYDFPWS + +IP YGGA +HDYHH +      N++S FTY D+I+GTDKG+
Sbjct: 213 IEAIETHSGYDFPWSLTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGY 272

BLAST of Cp4.1LG00g03620 vs. NCBI nr
Match: gi|449474027|ref|XP_004154053.1| (PREDICTED: methylsterol monooxygenase 2-2 [Cucumis sativus])

HSP 1 Score: 526.2 bits (1354), Expect = 3.3e-146
Identity = 245/253 (96.84%), Postives = 251/253 (99.21%), Query Frame = 1

Query: 3   QYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCI 62
           QYLITNFSDFQLACIGSFIIHESVFFLSGLPF+ LERAGWLSKYKIQAKNNSPAAQGKCI
Sbjct: 10  QYLITNFSDFQLACIGSFIIHESVFFLSGLPFILLERAGWLSKYKIQAKNNSPAAQGKCI 69

Query: 63  SRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRI 122
           SRLLLYHFGVNLPVM+VSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFI+EDFVFYWGHRI
Sbjct: 70  SRLLLYHFGVNLPVMLVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIIEDFVFYWGHRI 129

Query: 123 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVL 182
           LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATI+GPALTGPHL TLWLWMV+
Sbjct: 130 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIIGPALTGPHLFTLWLWMVV 189

Query: 183 RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 242
           RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK
Sbjct: 190 RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 249

Query: 243 GFRNLEAIKKAES 256
           GFRNLEAIKKAE+
Sbjct: 250 GFRNLEAIKKAEN 262

BLAST of Cp4.1LG00g03620 vs. NCBI nr
Match: gi|659115210|ref|XP_008457442.1| (PREDICTED: methylsterol monooxygenase 2-2 isoform X1 [Cucumis melo])

HSP 1 Score: 523.9 bits (1348), Expect = 1.6e-145
Identity = 245/253 (96.84%), Postives = 250/253 (98.81%), Query Frame = 1

Query: 3   QYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCI 62
           QYLITNFSDFQLACIGSFIIHESVFFLSGLPF+ LERAGWLSKYKIQAKNNSPAAQGKCI
Sbjct: 10  QYLITNFSDFQLACIGSFIIHESVFFLSGLPFILLERAGWLSKYKIQAKNNSPAAQGKCI 69

Query: 63  SRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRI 122
           SRLLLYHFGVNLPVM+VSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFI+EDFVFYWGHRI
Sbjct: 70  SRLLLYHFGVNLPVMLVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIIEDFVFYWGHRI 129

Query: 123 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVL 182
           LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL TLWLWMV+
Sbjct: 130 LHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLFTLWLWMVV 189

Query: 183 RVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 242
           RVLETVEAHCGY FPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK
Sbjct: 190 RVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDK 249

Query: 243 GFRNLEAIKKAES 256
           GFRNLEAIKKAE+
Sbjct: 250 GFRNLEAIKKAEN 262

BLAST of Cp4.1LG00g03620 vs. NCBI nr
Match: gi|659115212|ref|XP_008457443.1| (PREDICTED: methylsterol monooxygenase 2-2 isoform X2 [Cucumis melo])

HSP 1 Score: 523.9 bits (1348), Expect = 1.6e-145
Identity = 245/254 (96.46%), Postives = 251/254 (98.82%), Query Frame = 1

Query: 2   VQYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKC 61
           +QYLITNFSDFQLACIGSFIIHESVFFLSGLPF+ LERAGWLSKYKIQAKNNSPAAQGKC
Sbjct: 1   MQYLITNFSDFQLACIGSFIIHESVFFLSGLPFILLERAGWLSKYKIQAKNNSPAAQGKC 60

Query: 62  ISRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHR 121
           ISRLLLYHFGVNLPVM+VSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFI+EDFVFYWGHR
Sbjct: 61  ISRLLLYHFGVNLPVMLVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIIEDFVFYWGHR 120

Query: 122 ILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMV 181
           ILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL TLWLWMV
Sbjct: 121 ILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLFTLWLWMV 180

Query: 182 LRVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTD 241
           +RVLETVEAHCGY FPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTD
Sbjct: 181 VRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTD 240

Query: 242 KGFRNLEAIKKAES 256
           KGFRNLEAIKKAE+
Sbjct: 241 KGFRNLEAIKKAEN 254

BLAST of Cp4.1LG00g03620 vs. NCBI nr
Match: gi|567864526|ref|XP_006424912.1| (hypothetical protein CICLE_v10029052mg [Citrus clementina])

HSP 1 Score: 480.7 bits (1236), Expect = 1.6e-132
Identity = 222/251 (88.45%), Postives = 238/251 (94.82%), Query Frame = 1

Query: 1   MVQYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGK 60
           M QYLIT+FSDFQLAC+GSF +HES+FFL+GLPF++LERAGWLSKYKIQ KNNSPAAQ K
Sbjct: 1   MTQYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKNNSPAAQEK 60

Query: 61  CISRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGH 120
           CI+RLLLYHFGVNLPVMI SYPVFK MGM+S+LPLPSWKVV  QIIFYFI+EDFVFYWGH
Sbjct: 61  CITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILEDFVFYWGH 120

Query: 121 RILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWM 180
           RILHTKWLYK+VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHLMTLWLWM
Sbjct: 121 RILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWM 180

Query: 181 VLRVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGT 240
           VLRVLETVEAHCGY FPWS SNFIPLYGGA FHDYHHRLLYTKSGNYSSTF YMDWIFGT
Sbjct: 181 VLRVLETVEAHCGYHFPWSLSNFIPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 240

Query: 241 DKGFRNLEAIK 252
           DKG+R L+A+K
Sbjct: 241 DKGYRKLKALK 251

BLAST of Cp4.1LG00g03620 vs. NCBI nr
Match: gi|1009114727|ref|XP_015873842.1| (PREDICTED: methylsterol monooxygenase 2-2 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 478.0 bits (1229), Expect = 1.0e-131
Identity = 221/249 (88.76%), Postives = 238/249 (95.58%), Query Frame = 1

Query: 4   YLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNNSPAAQGKCIS 63
           YLIT+FSDFQLAC+GSF +HESVFFLSGLPF++LERAGWLSKYKIQ KNNSPAAQ KCI+
Sbjct: 11  YLITHFSDFQLACLGSFFLHESVFFLSGLPFIYLERAGWLSKYKIQTKNNSPAAQEKCIT 70

Query: 64  RLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRIL 123
           RLLLYHFGVNLPVMI SYPVF+ MGM+S+LPLPSWKVV  QIIFYFI+EDFVFYWGHRIL
Sbjct: 71  RLLLYHFGVNLPVMIFSYPVFRYMGMQSSLPLPSWKVVLTQIIFYFILEDFVFYWGHRIL 130

Query: 124 HTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLR 183
           HTKWLYK+VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL+TLWLWMVLR
Sbjct: 131 HTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLITLWLWMVLR 190

Query: 184 VLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKG 243
           VLETVEAHCGY FPWS SNF+PLYGGA FHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKG
Sbjct: 191 VLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKG 250

Query: 244 FRNLEAIKK 253
           +R L+A+KK
Sbjct: 251 YRKLKALKK 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SMO22_ARATH3.0e-13185.60Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana GN=SMO2-2 PE=2 SV=1[more]
SMO21_ARATH2.2e-12680.15Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana GN=SMO2-1 PE=2 SV=2[more]
MSMOB_DICDI7.9e-6045.87Putative methylsterol monooxygenase DDB_G0269788 OS=Dictyostelium discoideum GN=... [more]
SMO11_ARATH8.8e-5945.06Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana GN=SMO1-1 PE=1 SV=1[more]
SMO13_ARATH1.3e-5744.73Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana GN=SMO1-3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LVE5_CUCSA2.3e-14696.84Uncharacterized protein OS=Cucumis sativus GN=Csa_1G524750 PE=3 SV=1[more]
V4SCC9_9ROSI1.1e-13288.45Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029052mg PE=3 SV=1[more]
A0A067FH15_CITSI9.2e-13288.35Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g024309mg PE=3 SV=1[more]
V4UFS8_9ROSI9.2e-13288.35Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029052mg PE=3 SV=1[more]
A0A067L4U4_JATCU2.3e-13087.90Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02933 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G07420.11.7e-13285.60 sterol 4-alpha-methyl-oxidase 2-1[more]
AT2G29390.11.2e-12780.15 sterol 4-alpha-methyl-oxidase 2-2[more]
AT4G12110.14.9e-6045.06 sterol-4alpha-methyl oxidase 1-1[more]
AT4G22753.17.1e-5944.73 sterol 4-alpha methyl oxidase 1-3[more]
AT4G22756.16.0e-5844.81 sterol C4-methyl oxidase 1-2[more]
Match NameE-valueIdentityDescription
gi|449474027|ref|XP_004154053.1|3.3e-14696.84PREDICTED: methylsterol monooxygenase 2-2 [Cucumis sativus][more]
gi|659115210|ref|XP_008457442.1|1.6e-14596.84PREDICTED: methylsterol monooxygenase 2-2 isoform X1 [Cucumis melo][more]
gi|659115212|ref|XP_008457443.1|1.6e-14596.46PREDICTED: methylsterol monooxygenase 2-2 isoform X2 [Cucumis melo][more]
gi|567864526|ref|XP_006424912.1|1.6e-13288.45hypothetical protein CICLE_v10029052mg [Citrus clementina][more]
gi|1009114727|ref|XP_015873842.1|1.0e-13188.76PREDICTED: methylsterol monooxygenase 2-2 isoform X1 [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006633fatty acid biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0005506iron ion binding
Vocabulary: INTERPRO
TermDefinition
IPR006694Fatty_acid_hydroxylase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0000254 C-4 methylsterol oxidase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0004497 monooxygenase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG00g03620.1Cp4.1LG00g03620.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 106..240
score: 1.7
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 3..254
score: 6.0E
NoneNo IPR availablePANTHERPTHR11863:SF27METHYLSTEROL MONOOXYGENASE 2-1-RELATEDcoord: 3..254
score: 6.0E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG00g03620Cp4.1LG20g08340Cucurbita pepo (Zucchini)cpecpeB008
The following block(s) are covering this gene:

None