Cp4.1LG00g00830 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG00g00830
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCp4.1LG00 : 2008088 .. 2011042 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTAGCAAAACAATTCACACAAACGCATTTGATTTCCTCTCTTCATTAAATTACCCTCTGCAGATCTTGTTCCCTCATAATTCCGGCCATGTCCGCCACCGCCAAGCTGCTTGTCGCCGATCTCGCTTCCACCGTCCACCAACTCCCTTCCCGCTTCGTCCGCCTCATCGACGACCGTCCAAACTTCTCCCATGTCCGAGCTCCGACCGGCTACTCTTTCCCTGTCATTGACCTCCACAACCTCCACGGCCCCTCTCGTGCTGATGTCGTCGCCCAAATTGGCCAAGCCTGTGAGCGCGACGGTTTCTTTCTGGTAATGGGTTGTTTCTAATTTGGGTAAAGTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGTTTTAATGGAGGGGTTTGGCTGTTTTAGGTGAAGAACCATGGGGTGCCGGAGGAGATGATGAATGGGATGCTGAGATTGGCTAGGGAGTTTTTCCGGCTGCCGGAGTGTGAGAGACTTAAGAGCTATTCCGATGATCCGACAAAGACGACGAGGCTTTCGACGAGTTTCAATGTGAAGACGGAGAAGGTGGCAAATTGGAGAGATTTCTTGAGACTCCATTGTTACCCTCTCGAGCTTTATGTCAATGAATGGCCCGACAATCCTCCATCCTTCAGGTAACAAACACTTTTCATTCATGTTATGTCTGTACACTGTCCAAGCCGTAAGCAGATGTTGTCCTCTTTGTACCTTCTCTTCTTCTGAGTTACTCTCAATTTTTTAAATGGTCTAATCTGGAGAGCCACACCATTGTAAAGGGTGGAGAGATTTGGAGAGCCACACCATTGTAAAGGGTGGAGAGATTTCTTCCCAACCAGGTAAGATCCCGCAATCCATCCATCTTTGGAGCCCAACCTTCTTGTTGAAATGGGATCCCACCAAATTCACCCTCTTTAGGTCCCACCATGTTGCTGACACATCATCTCATGTCCATGGGACCCCACCAAATTCACCCTCCTTAGGTCCCAGCATGTTCTTGATCGTCTCATGTCCACCCTCTTTGGAGCTCAGCCTCCTCACTAACATATTGCCTGATGTTTTGACTCTAATCTTTGACTTTCCCTTTAGAGCTTACCCTCAAGGTTTTTGAAATGTGTCTCTTGGTAAGAGGTTTCCACACCCAAAGAGTGTTTTGTTCTCCTCCCTTGCCGATGTGCGATCTCATAATCCACCCATCTTCGGGGCCATCGTTCTCGTTGGCACTCGTACTTTTCTCTGATCAATGTGGGACCCCCACCAAATCCACCCCCTTGAGGGCCAGCGTCCTTGTTAGCACATCGCCTCATGTCCACCCTGTTCGGGGCTCTCCTCCCTAACCGATGTGCGATCTTATAATCCACCTATCTTCAGGGCCAGCGTTCTCGTTGGCACTCGTTCTTTTCTCTAATCAATGTGGGACCCCACCAAATCCACCCCCTTCGGGTCTAGCGTTCTTGTTGTCACATCGCCTCATGTTTACCCTGTTCGGGGCTCACCCAATGTTTGGCTCTAATACCATATGTAATGGCTCAAGCGCACCGCTAGCAGATATTGTTCTTTCTTGGTTTTACTCTTTAAAAAGCCTCTGCTAATGAGATGTTTCCACACCCTTATAAAATATGTTTCGATCTTCTCCTCCCTAACATTTCTAACATAAAATATCCTTACTTGCCTTACACAGGAAGGAAGTGGCGGAGTATTGTACAAGTGTGAGGGAATTGACACTAAAGCTATTAGAGGCCATTTCTGAGAGCTTGGGGTTACCAAAAGACTCCATAGCCAATTCAATAGGAGGCCATGGCCAACACATGGCCTTGAACTACTACCCGCCATGTCCACAGCCAGAGCTCACCTTTGGCTTACCCGGCCACACCGACCCGAACCTCATCACTCTCCTCCTCCAAGACCAAGTCCCCGGCCTCCAAGCCCTCCACGACAACACTTGGTTCGCTCTCGACCCCATCCCTAACACCTTTATAATTAACGTCGGCGATCAAATGCAGGTATAACCCTCTTCTTATATATATATGTCAAAACAAAGATCATGAATATATAAATGATAATATTTATGTTTTTGTTTAGGCGTTGAGTAATGACAGATACAAGAGCGTGCTTCATCGGGCTGTTGTGAATAGTGAAACGGAGCGAATTTCGATCCCGACATTTTATTGTCCATCGCAGGACGCGATGATCGGGCCGTTTAAGGAGCTGGTCGACGACAAGAATAGAGCGGTTTATAGACAATTTACTTATTCAGAATATTACAAGACGTTTTGGAATGAAGGACTTGCAACCAAAAGATGCTTAGATTTGTTTAGAGTTGAATGATTTGCACACTCCAATTGATCCTTCCATGCTTAAGGGCTGCTCGCATCTCCGACAGAGGAGCTTTCTGAGCTGGTTGAGCTGATGTCGGGCCGCACAATGGCCTCCTCCATCAACGACCAACTGCCAAGCTTGACATGGGATGACAAGTTGACAGCGTATGGCTTGAAGACTGGCCAGCTGAGATGTTTATCATCATAGTTTATCCATAATGTTCTTATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

mRNA sequence

TGTAGCAAAACAATTCACACAAACGCATTTGATTTCCTCTCTTCATTAAATTACCCTCTGCAGATCTTGTTCCCTCATAATTCCGGCCATGTCCGCCACCGCCAAGCTGCTTGTCGCCGATCTCGCTTCCACCGTCCACCAACTCCCTTCCCGCTTCGTCCGCCTCATCGACGACCGTCCAAACTTCTCCCATGTCCGAGCTCCGACCGGCTACTCTTTCCCTGTCATTGACCTCCACAACCTCCACGGCCCCTCTCGTGCTGATGTCGTCGCCCAAATTGGCCAAGCCTGTGAGCGCGACGGTTTCTTTCTGGTGAAGAACCATGGGGTGCCGGAGGAGATGATGAATGGGATGCTGAGATTGGCTAGGGAGTTTTTCCGGCTGCCGGAGTGTGAGAGACTTAAGAGCTATTCCGATGATCCGACAAAGACGACGAGGCTTTCGACGAGTTTCAATGTGAAGACGGAGAAGCTATTAGAGGCCATTTCTGAGAGCTTGGGGTTACCAAAAGACTCCATAGCCAATTCAATAGGAGGCCATGGCCAACACATGGCCTTGAACTACTACCCGCCATGTCCACAGCCAGAGCTCACCTTTGGCTTACCCGGCCACACCGACCCGAACCTCATCACTCTCCTCCTCCAAGACCAAGTCCCCGGCCTCCAAGCCCTCCACGACAACACTTGGTTCGCTCTCGACCCCATCCCTAACACCTTTATAATTAACGTCGGCGATCAAATGCAGGCGTTGAGTAATGACAGATACAAGAGCGTGCTTCATCGGGCTGTTGTGAATAGTGAAACGGAGCGAATTTCGATCCCGACATTTTATTGTCCATCGCAGGACGCGATGATCGGGCCGTTTAAGGAGCTGGTCGACGACAAGAATAGAGCGGTTTATAGACAATTTACTTATTCAGAATATTACAAGACGTTTTGGAATGAAGGACTTGCAACCAAAAGATGCTTAGATTTGTTTAGAGTTGAATGATTTGCACACTCCAATTGATCCTTCCATGCTTAAGGGCTGCTCGCATCTCCGACAGAGGAGCTTTCTGAGCTGGTTGAGCTGATGTCGGGCCGCACAATGGCCTCCTCCATCAACGACCAACTGCCAAGCTTGACATGGGATGACAAGTTGACAGCGTATGGCTTGAAGACTGGCCAGCTGAGATGTTTATCATCATAGTTTATCCATAATGTTCTTATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Coding sequence (CDS)

ATGTCCGCCACCGCCAAGCTGCTTGTCGCCGATCTCGCTTCCACCGTCCACCAACTCCCTTCCCGCTTCGTCCGCCTCATCGACGACCGTCCAAACTTCTCCCATGTCCGAGCTCCGACCGGCTACTCTTTCCCTGTCATTGACCTCCACAACCTCCACGGCCCCTCTCGTGCTGATGTCGTCGCCCAAATTGGCCAAGCCTGTGAGCGCGACGGTTTCTTTCTGGTGAAGAACCATGGGGTGCCGGAGGAGATGATGAATGGGATGCTGAGATTGGCTAGGGAGTTTTTCCGGCTGCCGGAGTGTGAGAGACTTAAGAGCTATTCCGATGATCCGACAAAGACGACGAGGCTTTCGACGAGTTTCAATGTGAAGACGGAGAAGCTATTAGAGGCCATTTCTGAGAGCTTGGGGTTACCAAAAGACTCCATAGCCAATTCAATAGGAGGCCATGGCCAACACATGGCCTTGAACTACTACCCGCCATGTCCACAGCCAGAGCTCACCTTTGGCTTACCCGGCCACACCGACCCGAACCTCATCACTCTCCTCCTCCAAGACCAAGTCCCCGGCCTCCAAGCCCTCCACGACAACACTTGGTTCGCTCTCGACCCCATCCCTAACACCTTTATAATTAACGTCGGCGATCAAATGCAGGCGTTGAGTAATGACAGATACAAGAGCGTGCTTCATCGGGCTGTTGTGAATAGTGAAACGGAGCGAATTTCGATCCCGACATTTTATTGTCCATCGCAGGACGCGATGATCGGGCCGTTTAAGGAGCTGGTCGACGACAAGAATAGAGCGGTTTATAGACAATTTACTTATTCAGAATATTACAAGACGTTTTGGAATGAAGGACTTGCAACCAAAAGATGCTTAGATTTGTTTAGAGTTGAATGA

Protein sequence

MSATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADVVAQIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLSTSFNVKTEKLLEAISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQALHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDAMIGPFKELVDDKNRAVYRQFTYSEYYKTFWNEGLATKRCLDLFRVE
BLAST of Cp4.1LG00g00830 vs. Swiss-Prot
Match: DLO1_ARATH (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana GN=DLO1 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.2e-70
Identity = 155/348 (44.54%), Postives = 198/348 (56.90%), Query Frame = 1

Query: 2   SATAKLLVADLASTVHQLPSRFVRLIDDRPNFSH-------------------------- 61
           SA +KLLV+D AS+VH +PS +VR I DRPN S                           
Sbjct: 4   SAISKLLVSDFASSVH-IPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQ 63

Query: 62  --VRAPTGYSFPVIDLHNLHGPSRADVVAQIGQACERDGFFLVKNHGVPEEMMNGM--LR 121
               A + Y F  I  H +      D      Q   R+ F   ++  V     +     R
Sbjct: 64  QLASACSTYGFFQIKNHGV-----PDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTR 123

Query: 122 LA-------------REFFRL---PECERLKSYSDDPTK----TTRLSTSFNVKTEKLLE 181
           L+             R+F RL   P  + ++ +   P      T   +TS      +LLE
Sbjct: 124 LSTSFNVGADKVLNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLE 183

Query: 182 AISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPG 241
           AISESLGL  D I+N +G H QHMA NYYPPCP+PELT+GLPGH DP +IT+LLQDQV G
Sbjct: 184 AISESLGLESDHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSG 243

Query: 242 LQALHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPS 299
           LQ   D+ W A+ PIPNTFI+N+GDQMQ +SND+YKSVLHRAVVN+E ER+SIPTFY PS
Sbjct: 244 LQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPS 303

BLAST of Cp4.1LG00g00830 vs. Swiss-Prot
Match: DLO2_ARATH (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.6e-67
Identity = 115/186 (61.83%), Postives = 148/186 (79.57%), Query Frame = 1

Query: 115 TTRLSTSFNVKTEKLLEAISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPG 174
           T   +TS       LLEAISESLGL KD ++N+IG HGQHMA+NYYP CPQPELT+GLPG
Sbjct: 159 TAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPG 218

Query: 175 HTDPNLITLLLQDQVPGLQALHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAV 234
           H D NLIT+LLQD+V GLQ   D  W A++P+PNTFI+N+GDQMQ +SN++YKSVLHRAV
Sbjct: 219 HKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAV 278

Query: 235 VNSETERISIPTFYCPSQDAMIGPFKELVDDK--NRAVYRQFTYSEYYKTFWNEGLATKR 294
           VNS+ ERISIPTFYCPS+DA+I P +EL++++  + A+YR FTY+EY++ FW+    T+ 
Sbjct: 279 VNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTES 338

Query: 295 CLDLFR 299
           C+D F+
Sbjct: 339 CIDSFK 344

BLAST of Cp4.1LG00g00830 vs. Swiss-Prot
Match: DMR6_ARATH (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana GN=DMR6 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.6e-54
Identity = 99/174 (56.90%), Postives = 127/174 (72.99%), Query Frame = 1

Query: 128 KLLEAISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQD 187
           K+ E ISESLGL KD +   +G  GQHMA+NYYPPCP+PELT+GLP HTDPN +T+LLQD
Sbjct: 165 KIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQD 224

Query: 188 -QVPGLQALHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPT 247
             V GLQ L D  WFA++P P+ F+IN+GDQ+QALSN  YKSV HRAV N+E  R+S+ +
Sbjct: 225 TTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVAS 284

Query: 248 FYCPSQDAMIGPFK---ELVDDKNRAVYRQFTYSEYYKTFWNEGLATKRCLDLF 298
           F CP+  A++ P K   E  DD+ + VY+ FTY+EYYK FW+  L  + CL+ F
Sbjct: 285 FLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENF 338

BLAST of Cp4.1LG00g00830 vs. Swiss-Prot
Match: ACCH5_ARATH (1-aminocyclopropane-1-carboxylate oxidase homolog 5 OS=Arabidopsis thaliana GN=2A6 PE=2 SV=2)

HSP 1 Score: 168.3 bits (425), Expect = 1.3e-40
Identity = 106/329 (32.22%), Postives = 156/329 (47.42%), Query Frame = 1

Query: 12  LASTVHQLPSRFVRLIDDRPNFSHVRAPTGY-SFPVIDLH--NLHGPSRADVVAQIGQAC 71
           +AS + ++P+ F    D   +      P+   + P +DL   ++   SR  VV +IG A 
Sbjct: 61  VASGIKEIPAMFHTPPDTLTSLKQTAPPSQQLTIPTVDLKGGSMDLISRRSVVEKIGDAA 120

Query: 72  ERDGFFLVKNHGVPEEMMNGMLRLAREFFR-------------------------LPECE 131
           ER GFF V NHG+  E+M  M    R F                           L  C 
Sbjct: 121 ERWGFFQVVNHGISVEVMERMKEGIRRFHEQDPEVKKRFYSRDHTRDVLYYSNIDLHTCN 180

Query: 132 RLKSYSD--------DPTKTTRL-----------STSFNVKTEKLLEAISESLGLPKDSI 191
           +  ++ D        DP K   L           S       E L E +SE+LGL  + +
Sbjct: 181 KAANWRDTLACYMAPDPPKLQDLPAVCGEIMMEYSKQLMTLGEFLFELLSEALGLNPNHL 240

Query: 192 ANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQALHDNTWFALD 251
            +        M   YYPPCPQP+LT G+  HTD + IT+LLQD + GLQ +HD  W  + 
Sbjct: 241 KDMGCAKSHIMFGQYYPPCPQPDLTLGISKHTDFSFITILLQDNIGGLQVIHDQCWVDVS 300

Query: 252 PIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETE-RISIPTF---YCPSQDAMIGPFK 290
           P+P   +IN+GD +Q +SND++ S  HR + N  +E RIS+P F   +      + GP K
Sbjct: 301 PVPGALVINIGDLLQLISNDKFISAEHRVIANGSSEPRISMPCFVSTFMKPNPRIYGPIK 360

BLAST of Cp4.1LG00g00830 vs. Swiss-Prot
Match: F6H2_ARATH (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 6.3e-40
Identity = 101/328 (30.79%), Postives = 155/328 (47.26%), Query Frame = 1

Query: 19  LPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADVVAQIGQACERDGFFLVKN 78
           LP ++++  ++R    HV+  +  S PVID+ NL   S +  V     A E  GFF V N
Sbjct: 37  LPDQYIQPFEERLINFHVKEDSDESIPVIDISNLDEKSVSKAVCD---AAEEWGFFQVIN 96

Query: 79  HGVPEEMMNGMLRLAREFFRLP-------------------------ECERLKSYSD--- 138
           HGV  E++  M      FF LP                           E+   + D   
Sbjct: 97  HGVSMEVLENMKTATHRFFGLPVEEKRKFSREKSLSTNVRFGTSFSPHAEKALEWKDYLS 156

Query: 139 --------------DPTKTTRLSTSFNVK--TEKLLEAISESLGLPK-DSIANSIGGHGQ 198
                         D  ++  L      K   +KLL  + E+L + + D    S      
Sbjct: 157 LFFVSEAEASQLWPDSCRSETLEYMNETKPLVKKLLRFLGENLNVKELDKTKESFFMGST 216

Query: 199 HMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGL--QALHDNTWFALDPIPNTFI 258
            + LNYYP CP PELT G+  H+D + +T+LLQD++ GL  ++L    W  + PI  + +
Sbjct: 217 RINLNYYPICPNPELTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPISGSLV 276

Query: 259 INVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDAMIGPFKELVDDKNRAVY 300
           IN+GD MQ +SN RYKSV HR + N    RIS+P F  P  +++IGP  E++++  + VY
Sbjct: 277 INIGDAMQIMSNGRYKSVEHRVLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVY 336

BLAST of Cp4.1LG00g00830 vs. TrEMBL
Match: A0A058ZVW3_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L00789 PE=3 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 7.6e-109
Identity = 187/344 (54.36%), Postives = 241/344 (70.06%), Query Frame = 1

Query: 2   SATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADVV 61
           +A  KLL+ D    ++++P+ ++R   DRPN + V A    + P+IDL  L GP+R D++
Sbjct: 3   TAATKLLLIDRVPGINRVPNSYIRPQADRPNLTEVEASDASTIPLIDLQGLFGPNRDDII 62

Query: 62  AQIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLSTS 121
            QIG+AC+RDGFF +KNHG+PEE++  ++ +A EFFRLPE ERLK+YSDDP+K+TRLSTS
Sbjct: 63  RQIGKACQRDGFFQIKNHGIPEEVVQAIMNIAGEFFRLPESERLKNYSDDPSKSTRLSTS 122

Query: 122 FNVKTEKLL----------------------------------------------EAISE 181
           FN+KTEK+                                               EAISE
Sbjct: 123 FNIKTEKVSNWRDFLRLHCYPLEEYMHEWPTNPPSFRKEVGEYCTRVRELALKLLEAISE 182

Query: 182 SLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQAL 241
           SLGL ++ I+ ++G HGQHMA+NYYPPCPQPELT+GLPGHTD NLIT+LLQD+VPGLQ L
Sbjct: 183 SLGLEREYISKNLGKHGQHMAMNYYPPCPQPELTYGLPGHTDQNLITILLQDEVPGLQVL 242

Query: 242 HDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDAM 300
            D  W A++PIPNTFI+N+GDQMQ +SND+YKS+LHRAVVN + ERISIPTFYCPS +A+
Sbjct: 243 RDGKWIAVNPIPNTFIVNIGDQMQVISNDKYKSILHRAVVNCDRERISIPTFYCPSPEAL 302

BLAST of Cp4.1LG00g00830 vs. TrEMBL
Match: M5W9T9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008091mg PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.2e-107
Identity = 196/344 (56.98%), Postives = 239/344 (69.48%), Query Frame = 1

Query: 1   MSATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADV 60
           M+   KLL+ DL S V+ +PS +VR I DRPN S V+     S P+IDL +LHG + +++
Sbjct: 1   MATATKLLLTDLMSGVNHVPSNYVRPISDRPNLSDVQISDA-SIPLIDLKDLHGHNHSNI 60

Query: 61  VAQIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLST 120
           + QIG AC+ DGFF VKNHGVPEEM+  ML +AREFF+LPE ERLK YSDDP+KTTRLST
Sbjct: 61  IKQIGLACQTDGFFQVKNHGVPEEMIKDMLSIAREFFKLPESERLKMYSDDPSKTTRLST 120

Query: 121 SFNVKTEK----------------------------------------------LLEAIS 180
           SFNV+TEK                                              LL AIS
Sbjct: 121 SFNVRTEKLSNWRDFLRLHCYPLEDYVQEWPNNPPSFREQVGEYCTTVRGLVLRLLGAIS 180

Query: 181 ESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQA 240
           ESLGL K+ I  ++G  GQHMALNYYPPCP+PELT+GLPGHTD NLIT+LLQD V GLQ 
Sbjct: 181 ESLGLEKNYIVEALGKQGQHMALNYYPPCPEPELTYGLPGHTDCNLITILLQDDVAGLQV 240

Query: 241 LHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDA 299
           L +  W A++PIPNTFI+N+GD MQ +SND+YKSVLHRAVVN ++ERISIPTFYCPS DA
Sbjct: 241 LRNGKWVAVNPIPNTFIVNIGDMMQVISNDKYKSVLHRAVVNCKSERISIPTFYCPSPDA 300

BLAST of Cp4.1LG00g00830 vs. TrEMBL
Match: M5WF25_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008100mg PE=3 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 4.2e-107
Identity = 196/344 (56.98%), Postives = 238/344 (69.19%), Query Frame = 1

Query: 1   MSATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADV 60
           M+   KLL+ DL S V+ +PS +VR I DRPN S V+     S P+IDL +LHG + +++
Sbjct: 1   MATATKLLLTDLMSGVNHVPSNYVRPISDRPNLSDVQISDA-SIPLIDLKDLHGHNHSNI 60

Query: 61  VAQIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLST 120
           + QIG AC+ DGFF VKNHGVPEEM+  ML +AREFF+LPE ERLK YSDDP+KTTRLST
Sbjct: 61  IKQIGLACQTDGFFQVKNHGVPEEMIKDMLSIAREFFKLPESERLKMYSDDPSKTTRLST 120

Query: 121 SFNVKTEK----------------------------------------------LLEAIS 180
           SFNV+TEK                                              LL AIS
Sbjct: 121 SFNVRTEKLSNWRDFLRLHCYPLEDYVQEWPNNPPSFREQVGEYCTTVRGLVLRLLGAIS 180

Query: 181 ESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQA 240
           ESLGL K+ I  ++G  GQHMALNYYPPCP+PELT+GLPGHTD NLIT+LLQD V GLQ 
Sbjct: 181 ESLGLEKNYIVEALGKQGQHMALNYYPPCPEPELTYGLPGHTDCNLITILLQDDVAGLQV 240

Query: 241 LHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDA 299
           L +  W A++PIPNTFI+N+GD MQ +SND+YKSVLHRAVVN  +ERISIPTFYCPS DA
Sbjct: 241 LRNGKWVAVNPIPNTFIVNIGDMMQVISNDKYKSVLHRAVVNCNSERISIPTFYCPSPDA 300

BLAST of Cp4.1LG00g00830 vs. TrEMBL
Match: A0A058ZVN3_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L00786 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 5.4e-107
Identity = 184/344 (53.49%), Postives = 240/344 (69.77%), Query Frame = 1

Query: 2   SATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADVV 61
           +A  KLL+ D    ++++P+ ++R   DRPN + V A    + P+IDL  L GP+R D++
Sbjct: 3   TAATKLLLIDRVPGINRVPNSYIRPEADRPNLTEVEASDASTIPLIDLQGLFGPNRDDII 62

Query: 62  AQIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLSTS 121
             IG+AC++DGFF +KNHG+PEE++  ++ +A EFFRLPE ERLK+YSDDP+K+TRLSTS
Sbjct: 63  RLIGEACQQDGFFQIKNHGIPEEVVQAIMNIAGEFFRLPESERLKNYSDDPSKSTRLSTS 122

Query: 122 FNVKTEKLL----------------------------------------------EAISE 181
           FN+KTEK+                                               EAISE
Sbjct: 123 FNIKTEKVSNWRDFLRLHCYPLEEYMHEWPTNPPSFRKEVGEYCTRVRELALKLLEAISE 182

Query: 182 SLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQAL 241
           SLGL ++ I+ ++G HGQHMA+NYYPPCPQPELT+GLPGHTD NLIT+LLQD VPGLQ L
Sbjct: 183 SLGLEREYISKNLGKHGQHMAMNYYPPCPQPELTYGLPGHTDRNLITILLQDDVPGLQVL 242

Query: 242 HDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDAM 300
            D  W A++PIPNTFI+N+GDQMQ +SND+YKS+LHRAVVN + ERISIPTFYCPS +A+
Sbjct: 243 RDGKWIAVNPIPNTFIVNIGDQMQVISNDKYKSILHRAVVNCDRERISIPTFYCPSPEAL 302

BLAST of Cp4.1LG00g00830 vs. TrEMBL
Match: A0A058ZVM4_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L00350 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 9.6e-104
Identity = 185/343 (53.94%), Postives = 236/343 (68.80%), Query Frame = 1

Query: 3   ATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADVVA 62
           A AK+L+ DLAS + ++P  ++R   DRPN + V A    S P++DL  L GP+R D++ 
Sbjct: 4   AAAKVLLTDLASGIDRVPDNYIRSEADRPNLTEVEASDASSIPLVDLQGLSGPNRDDIIR 63

Query: 63  QIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLSTSF 122
           QIG+AC++DGFF +KNHG+PEE +  ++ +AREFFRLPE ERLK+YSDDPTK+TRLSTSF
Sbjct: 64  QIGRACQQDGFFQIKNHGIPEEKVQAIMNIAREFFRLPESERLKNYSDDPTKSTRLSTSF 123

Query: 123 NVKTEKL-------------LE----------------------AISESLGLPKDSIANS 182
           N+KTEK+             LE                       + E +    ++I+ S
Sbjct: 124 NLKTEKVSNWRDFLRLHCYPLEEYMHEWPTNPPSFRKEVGEYCTRVRELVFKLLEAISES 183

Query: 183 IG-----------GHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQALH 242
           +G            HGQHMA+NYYPPCPQPELT+GLPGHTDPNLIT+LLQD VPGLQ L 
Sbjct: 184 LGLEREYISQNLGKHGQHMAMNYYPPCPQPELTYGLPGHTDPNLITILLQDDVPGLQVLR 243

Query: 243 DNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDAMI 300
           +  W A++PIPNTFI+N+GDQMQ +SND+YKSVLHRAVVN  TERISIPTFYCPS + +I
Sbjct: 244 NGKWVAVNPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNCNTERISIPTFYCPSPEGLI 303

BLAST of Cp4.1LG00g00830 vs. TAIR10
Match: AT4G10500.1 (AT4G10500.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 268.1 bits (684), Expect = 6.6e-72
Identity = 155/348 (44.54%), Postives = 198/348 (56.90%), Query Frame = 1

Query: 2   SATAKLLVADLASTVHQLPSRFVRLIDDRPNFSH-------------------------- 61
           SA +KLLV+D AS+VH +PS +VR I DRPN S                           
Sbjct: 4   SAISKLLVSDFASSVH-IPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQ 63

Query: 62  --VRAPTGYSFPVIDLHNLHGPSRADVVAQIGQACERDGFFLVKNHGVPEEMMNGM--LR 121
               A + Y F  I  H +      D      Q   R+ F   ++  V     +     R
Sbjct: 64  QLASACSTYGFFQIKNHGV-----PDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTR 123

Query: 122 LA-------------REFFRL---PECERLKSYSDDPTK----TTRLSTSFNVKTEKLLE 181
           L+             R+F RL   P  + ++ +   P      T   +TS      +LLE
Sbjct: 124 LSTSFNVGADKVLNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLE 183

Query: 182 AISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPG 241
           AISESLGL  D I+N +G H QHMA NYYPPCP+PELT+GLPGH DP +IT+LLQDQV G
Sbjct: 184 AISESLGLESDHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSG 243

Query: 242 LQALHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPS 299
           LQ   D+ W A+ PIPNTFI+N+GDQMQ +SND+YKSVLHRAVVN+E ER+SIPTFY PS
Sbjct: 244 LQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPS 303

BLAST of Cp4.1LG00g00830 vs. TAIR10
Match: AT4G10490.1 (AT4G10490.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 257.7 bits (657), Expect = 9.0e-69
Identity = 115/186 (61.83%), Postives = 148/186 (79.57%), Query Frame = 1

Query: 115 TTRLSTSFNVKTEKLLEAISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPG 174
           T   +TS       LLEAISESLGL KD ++N+IG HGQHMA+NYYP CPQPELT+GLPG
Sbjct: 159 TAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPG 218

Query: 175 HTDPNLITLLLQDQVPGLQALHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAV 234
           H D NLIT+LLQD+V GLQ   D  W A++P+PNTFI+N+GDQMQ +SN++YKSVLHRAV
Sbjct: 219 HKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAV 278

Query: 235 VNSETERISIPTFYCPSQDAMIGPFKELVDDK--NRAVYRQFTYSEYYKTFWNEGLATKR 294
           VNS+ ERISIPTFYCPS+DA+I P +EL++++  + A+YR FTY+EY++ FW+    T+ 
Sbjct: 279 VNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFDTES 338

Query: 295 CLDLFR 299
           C+D F+
Sbjct: 339 CIDSFK 344

BLAST of Cp4.1LG00g00830 vs. TAIR10
Match: AT5G24530.1 (AT5G24530.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 213.8 bits (543), Expect = 1.5e-55
Identity = 99/174 (56.90%), Postives = 127/174 (72.99%), Query Frame = 1

Query: 128 KLLEAISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQD 187
           K+ E ISESLGL KD +   +G  GQHMA+NYYPPCP+PELT+GLP HTDPN +T+LLQD
Sbjct: 165 KIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQD 224

Query: 188 -QVPGLQALHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPT 247
             V GLQ L D  WFA++P P+ F+IN+GDQ+QALSN  YKSV HRAV N+E  R+S+ +
Sbjct: 225 TTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVAS 284

Query: 248 FYCPSQDAMIGPFK---ELVDDKNRAVYRQFTYSEYYKTFWNEGLATKRCLDLF 298
           F CP+  A++ P K   E  DD+ + VY+ FTY+EYYK FW+  L  + CL+ F
Sbjct: 285 FLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENF 338

BLAST of Cp4.1LG00g00830 vs. TAIR10
Match: AT1G03410.1 (AT1G03410.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 168.3 bits (425), Expect = 7.1e-42
Identity = 106/329 (32.22%), Postives = 156/329 (47.42%), Query Frame = 1

Query: 12  LASTVHQLPSRFVRLIDDRPNFSHVRAPTGY-SFPVIDLH--NLHGPSRADVVAQIGQAC 71
           +AS + ++P+ F    D   +      P+   + P +DL   ++   SR  VV +IG A 
Sbjct: 61  VASGIKEIPAMFHTPPDTLTSLKQTAPPSQQLTIPTVDLKGGSMDLISRRSVVEKIGDAA 120

Query: 72  ERDGFFLVKNHGVPEEMMNGMLRLAREFFR-------------------------LPECE 131
           ER GFF V NHG+  E+M  M    R F                           L  C 
Sbjct: 121 ERWGFFQVVNHGISVEVMERMKEGIRRFHEQDPEVKKRFYSRDHTRDVLYYSNIDLHTCN 180

Query: 132 RLKSYSD--------DPTKTTRL-----------STSFNVKTEKLLEAISESLGLPKDSI 191
           +  ++ D        DP K   L           S       E L E +SE+LGL  + +
Sbjct: 181 KAANWRDTLACYMAPDPPKLQDLPAVCGEIMMEYSKQLMTLGEFLFELLSEALGLNPNHL 240

Query: 192 ANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQALHDNTWFALD 251
            +        M   YYPPCPQP+LT G+  HTD + IT+LLQD + GLQ +HD  W  + 
Sbjct: 241 KDMGCAKSHIMFGQYYPPCPQPDLTLGISKHTDFSFITILLQDNIGGLQVIHDQCWVDVS 300

Query: 252 PIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETE-RISIPTF---YCPSQDAMIGPFK 290
           P+P   +IN+GD +Q +SND++ S  HR + N  +E RIS+P F   +      + GP K
Sbjct: 301 PVPGALVINIGDLLQLISNDKFISAEHRVIANGSSEPRISMPCFVSTFMKPNPRIYGPIK 360

BLAST of Cp4.1LG00g00830 vs. TAIR10
Match: AT3G21420.1 (AT3G21420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 167.5 bits (423), Expect = 1.2e-41
Identity = 110/346 (31.79%), Postives = 166/346 (47.98%), Query Frame = 1

Query: 12  LASTVHQLPSRFVR------LIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADV---VA 71
           + S  +++P RF+R      ++       H+     +  PVIDL  L  P   D    + 
Sbjct: 21  IKSKPNKVPERFIREEYERGVVVSSLKTHHLH----HQIPVIDLSKLSKPDNDDFFFEIL 80

Query: 72  QIGQACERDGFFLVKNHGVPEE------------------------MMNGMLRLAREFFR 131
           ++ QACE  GFF V NHG+  E                        M  G ++   + F 
Sbjct: 81  KLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPGTVQGYGQAFI 140

Query: 132 LPECERL---------------KSYSDDPTKTTRLSTSFNVKTE-------KLLEAISES 191
             E ++L               ++    P+K  R S S    ++       +LL+ I+ S
Sbjct: 141 FSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLKYIAIS 200

Query: 192 LGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQ--VPGLQA 251
           LGL ++      G   Q + +NYYPPC  P+L  GL  H+D + +T+L Q +    GLQ 
Sbjct: 201 LGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSCVGLQI 260

Query: 252 LHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDA 300
           L DNTW  + P+PN  +IN+GD ++ LSN +YKSV HRAV N E ER++I TFY P+ + 
Sbjct: 261 LKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYAPNYEV 320

BLAST of Cp4.1LG00g00830 vs. NCBI nr
Match: gi|470147895|ref|XP_004309513.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 409.5 bits (1051), Expect = 5.2e-111
Identity = 200/341 (58.65%), Postives = 237/341 (69.50%), Query Frame = 1

Query: 4   TAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADVVAQ 63
           TAKLL+ DL   V  +PS ++R I DRPN + V+A +  S P+IDL  L+GP+ +D++ Q
Sbjct: 3   TAKLLLTDLIPVVDHVPSNYIRPISDRPNLADVQASSEGSIPLIDLQGLNGPNHSDIIQQ 62

Query: 64  IGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLSTSFN 123
           I QAC+ DGFF VKNHGVP EM++GML +AREFFRLPE ER+K YSDDP+KTTRLSTSFN
Sbjct: 63  IAQACQNDGFFQVKNHGVPAEMIDGMLNIAREFFRLPESERMKMYSDDPSKTTRLSTSFN 122

Query: 124 VKTE----------------------------------------------KLLEAISESL 183
           VKTE                                              KLLEAISESL
Sbjct: 123 VKTEKTANWRDFLRLHCYPLEDYVHEWPSNPPSFREEVGEYCTSVRGLVLKLLEAISESL 182

Query: 184 GLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQALHD 243
           GL KD I  ++G   QHMALNYYPPCP+PELT+GLPGHTD NLIT+LLQD VPGLQ L  
Sbjct: 183 GLQKDYIVKALGKQAQHMALNYYPPCPEPELTYGLPGHTDGNLITILLQDDVPGLQVLRK 242

Query: 244 NTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDAMIG 299
             W A++PIPNT+I+N+GDQMQ +SNDRYKSVLHRAVVN   ERIS+PTFYCPS DA+IG
Sbjct: 243 GKWVAVNPIPNTYIVNIGDQMQVISNDRYKSVLHRAVVNYNKERISVPTFYCPSPDALIG 302

BLAST of Cp4.1LG00g00830 vs. NCBI nr
Match: gi|702508029|ref|XP_010040087.1| (PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Eucalyptus grandis])

HSP 1 Score: 401.7 bits (1031), Expect = 1.1e-108
Identity = 187/344 (54.36%), Postives = 241/344 (70.06%), Query Frame = 1

Query: 2   SATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADVV 61
           +A  KLL+ D    ++++P+ ++R   DRPN + V A    + P+IDL  L GP+R D++
Sbjct: 3   TAATKLLLIDRVPGINRVPNSYIRPQADRPNLTEVEASDASTIPLIDLQGLFGPNRDDII 62

Query: 62  AQIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLSTS 121
            QIG+AC+RDGFF +KNHG+PEE++  ++ +A EFFRLPE ERLK+YSDDP+K+TRLSTS
Sbjct: 63  RQIGKACQRDGFFQIKNHGIPEEVVQAIMNIAGEFFRLPESERLKNYSDDPSKSTRLSTS 122

Query: 122 FNVKTEKLL----------------------------------------------EAISE 181
           FN+KTEK+                                               EAISE
Sbjct: 123 FNIKTEKVSNWRDFLRLHCYPLEEYMHEWPTNPPSFRKEVGEYCTRVRELALKLLEAISE 182

Query: 182 SLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQAL 241
           SLGL ++ I+ ++G HGQHMA+NYYPPCPQPELT+GLPGHTD NLIT+LLQD+VPGLQ L
Sbjct: 183 SLGLEREYISKNLGKHGQHMAMNYYPPCPQPELTYGLPGHTDQNLITILLQDEVPGLQVL 242

Query: 242 HDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDAM 300
            D  W A++PIPNTFI+N+GDQMQ +SND+YKS+LHRAVVN + ERISIPTFYCPS +A+
Sbjct: 243 RDGKWIAVNPIPNTFIVNIGDQMQVISNDKYKSILHRAVVNCDRERISIPTFYCPSPEAL 302

BLAST of Cp4.1LG00g00830 vs. NCBI nr
Match: gi|645290121|ref|XP_008245738.1| (PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Prunus mume])

HSP 1 Score: 396.7 bits (1018), Expect = 3.5e-107
Identity = 193/344 (56.10%), Postives = 237/344 (68.90%), Query Frame = 1

Query: 1   MSATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADV 60
           M+   KLL+ DL S V+ +PS +VR I DRPN   V+     S P+IDL +LHG + +++
Sbjct: 1   MATATKLLLTDLMSGVNHVPSNYVRPISDRPNLRDVQISDASSIPLIDLKDLHGHNHSNI 60

Query: 61  VAQIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLST 120
           + QIG AC+ DGFF VKNHGVPEEM+  M  +AR+FF+LPE ERLK YSDDP+KTTRLST
Sbjct: 61  IKQIGLACQTDGFFQVKNHGVPEEMIKDMSSIARKFFKLPESERLKMYSDDPSKTTRLST 120

Query: 121 SFNVKTEK----------------------------------------------LLEAIS 180
           SFNV+TEK                                              LL AIS
Sbjct: 121 SFNVRTEKLSNWRDFLRLHCYPLEDYVQEWPNNPPSFREQVGEYCTSVRGLVLRLLGAIS 180

Query: 181 ESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQA 240
           ESLGL K+ I  ++G  GQHMALNYYPPCP+PELT+GLPGHTD NLIT+LLQD VPGLQ 
Sbjct: 181 ESLGLEKNYIVEALGKQGQHMALNYYPPCPEPELTYGLPGHTDCNLITILLQDDVPGLQV 240

Query: 241 LHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDA 299
           L +  W A++PIPNTFI+N+GD MQ +SND+YKSVLHRAVVN  +ERISIPTFYCPS DA
Sbjct: 241 LRNGKWVAVNPIPNTFIVNIGDMMQVISNDKYKSVLHRAVVNCNSERISIPTFYCPSPDA 300

BLAST of Cp4.1LG00g00830 vs. NCBI nr
Match: gi|595847248|ref|XP_007209285.1| (hypothetical protein PRUPE_ppa008091mg [Prunus persica])

HSP 1 Score: 396.4 bits (1017), Expect = 4.6e-107
Identity = 196/344 (56.98%), Postives = 239/344 (69.48%), Query Frame = 1

Query: 1   MSATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADV 60
           M+   KLL+ DL S V+ +PS +VR I DRPN S V+     S P+IDL +LHG + +++
Sbjct: 1   MATATKLLLTDLMSGVNHVPSNYVRPISDRPNLSDVQISDA-SIPLIDLKDLHGHNHSNI 60

Query: 61  VAQIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLST 120
           + QIG AC+ DGFF VKNHGVPEEM+  ML +AREFF+LPE ERLK YSDDP+KTTRLST
Sbjct: 61  IKQIGLACQTDGFFQVKNHGVPEEMIKDMLSIAREFFKLPESERLKMYSDDPSKTTRLST 120

Query: 121 SFNVKTEK----------------------------------------------LLEAIS 180
           SFNV+TEK                                              LL AIS
Sbjct: 121 SFNVRTEKLSNWRDFLRLHCYPLEDYVQEWPNNPPSFREQVGEYCTTVRGLVLRLLGAIS 180

Query: 181 ESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQA 240
           ESLGL K+ I  ++G  GQHMALNYYPPCP+PELT+GLPGHTD NLIT+LLQD V GLQ 
Sbjct: 181 ESLGLEKNYIVEALGKQGQHMALNYYPPCPEPELTYGLPGHTDCNLITILLQDDVAGLQV 240

Query: 241 LHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDA 299
           L +  W A++PIPNTFI+N+GD MQ +SND+YKSVLHRAVVN ++ERISIPTFYCPS DA
Sbjct: 241 LRNGKWVAVNPIPNTFIVNIGDMMQVISNDKYKSVLHRAVVNCKSERISIPTFYCPSPDA 300

BLAST of Cp4.1LG00g00830 vs. NCBI nr
Match: gi|595860692|ref|XP_007211193.1| (hypothetical protein PRUPE_ppa008100mg [Prunus persica])

HSP 1 Score: 396.0 bits (1016), Expect = 6.0e-107
Identity = 196/344 (56.98%), Postives = 238/344 (69.19%), Query Frame = 1

Query: 1   MSATAKLLVADLASTVHQLPSRFVRLIDDRPNFSHVRAPTGYSFPVIDLHNLHGPSRADV 60
           M+   KLL+ DL S V+ +PS +VR I DRPN S V+     S P+IDL +LHG + +++
Sbjct: 1   MATATKLLLTDLMSGVNHVPSNYVRPISDRPNLSDVQISDA-SIPLIDLKDLHGHNHSNI 60

Query: 61  VAQIGQACERDGFFLVKNHGVPEEMMNGMLRLAREFFRLPECERLKSYSDDPTKTTRLST 120
           + QIG AC+ DGFF VKNHGVPEEM+  ML +AREFF+LPE ERLK YSDDP+KTTRLST
Sbjct: 61  IKQIGLACQTDGFFQVKNHGVPEEMIKDMLSIAREFFKLPESERLKMYSDDPSKTTRLST 120

Query: 121 SFNVKTEK----------------------------------------------LLEAIS 180
           SFNV+TEK                                              LL AIS
Sbjct: 121 SFNVRTEKLSNWRDFLRLHCYPLEDYVQEWPNNPPSFREQVGEYCTTVRGLVLRLLGAIS 180

Query: 181 ESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTFGLPGHTDPNLITLLLQDQVPGLQA 240
           ESLGL K+ I  ++G  GQHMALNYYPPCP+PELT+GLPGHTD NLIT+LLQD V GLQ 
Sbjct: 181 ESLGLEKNYIVEALGKQGQHMALNYYPPCPEPELTYGLPGHTDCNLITILLQDDVAGLQV 240

Query: 241 LHDNTWFALDPIPNTFIINVGDQMQALSNDRYKSVLHRAVVNSETERISIPTFYCPSQDA 299
           L +  W A++PIPNTFI+N+GD MQ +SND+YKSVLHRAVVN  +ERISIPTFYCPS DA
Sbjct: 241 LRNGKWVAVNPIPNTFIVNIGDMMQVISNDKYKSVLHRAVVNCNSERISIPTFYCPSPDA 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
DLO1_ARATH1.2e-7044.54Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana GN=DLO1 PE=1 SV=1[more]
DLO2_ARATH1.6e-6761.83Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1[more]
DMR6_ARATH2.6e-5456.90Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana GN=DMR6 PE=1 SV=1[more]
ACCH5_ARATH1.3e-4032.221-aminocyclopropane-1-carboxylate oxidase homolog 5 OS=Arabidopsis thaliana GN=2... [more]
F6H2_ARATH6.3e-4030.79Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A058ZVW3_EUCGR7.6e-10954.36Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L00789 PE=3 SV=1[more]
M5W9T9_PRUPE3.2e-10756.98Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008091mg PE=3 SV=1[more]
M5WF25_PRUPE4.2e-10756.98Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008100mg PE=3 SV=1[more]
A0A058ZVN3_EUCGR5.4e-10753.49Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L00786 PE=3 SV=1[more]
A0A058ZVM4_EUCGR9.6e-10453.94Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L00350 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G10500.16.6e-7244.54 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G10490.19.0e-6961.83 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G24530.11.5e-5556.90 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G03410.17.1e-4232.22 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT3G21420.11.2e-4131.79 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
Match NameE-valueIdentityDescription
gi|470147895|ref|XP_004309513.1|5.2e-11158.65PREDICTED: hyoscyamine 6-dioxygenase-like [Fragaria vesca subsp. vesca][more]
gi|702508029|ref|XP_010040087.1|1.1e-10854.36PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Eucalyptus grandis][more]
gi|645290121|ref|XP_008245738.1|3.5e-10756.10PREDICTED: hyoscyamine 6-dioxygenase-like isoform X2 [Prunus mume][more]
gi|595847248|ref|XP_007209285.1|4.6e-10756.98hypothetical protein PRUPE_ppa008091mg [Prunus persica][more]
gi|595860692|ref|XP_007211193.1|6.0e-10756.98hypothetical protein PRUPE_ppa008100mg [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
IPR005123Oxoglu/Fe-dep_dioxygenase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009813 flavonoid biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0005506 iron ion binding
molecular_function GO:0045486 naringenin 3-dioxygenase activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG00g00830.1Cp4.1LG00g00830.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 153..250
score: 4.3
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 151..250
score: 14
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 45..136
score: 6.1
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 14..126
score: 6.0E-24coord: 127..296
score: 2.3
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 9..293
score: 6.8E
NoneNo IPR availablePANTHERPTHR10209:SF196F3H7.17 PROTEINcoord: 9..293
score: 6.8E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 13..288
score: 2.88