CmoCh19G004630 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh19G004630
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Cytochrome P450 family ent-kaurenoic acid oxidase
Location	Cmo_Chr19 : 5592799 .. 5593085 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAACGATTAATGTTTGTATAATTATTGGATTTCTTTTAGGGGTTTACATGTTTGTGAGGAAACTCAATGAAATATGGTATTTGGTTAAGTTGGGAAGAAAGGCTTACAAATCTCTTCCTCCTGGTGATATGGGATGGCCTCTTCTTGGCTCCTCCTTCTCCTTTTACAAAGCATTTATAGTCGGAGGTGATCCCGACTCTTTCATTTATCACCTTCTTTCCAGGTATGTTTCTTCATCTCTCTTTTTCAGGTCATATGTATTGTTATTAGGTGCATATCATGA

mRNA sequence

ATGGAAACGATTAATGTTTGTATAATTATTGGATTTCTTTTAGGGGTTTACATGTTTGTGAGGAAACTCAATGAAATATGGTATTTGGTTAAGTTGGGAAGAAAGGCTTACAAATCTCTTCCTCCTGGTGATATGGGATGGCCTCTTCTTGGCTCCTCCTTCTCCTTTTACAAAGCATTTATAGTCGGAGGTGATCCCGACTCTTTCATTTATCACCTTCTTTCCAGGTGCATATCATGA

Coding sequence (CDS)

ATGGAAACGATTAATGTTTGTATAATTATTGGATTTCTTTTAGGGGTTTACATGTTTGTGAGGAAACTCAATGAAATATGGTATTTGGTTAAGTTGGGAAGAAAGGCTTACAAATCTCTTCCTCCTGGTGATATGGGATGGCCTCTTCTTGGCTCCTCCTTCTCCTTTTACAAAGCATTTATAGTCGGAGGTGATCCCGACTCTTTCATTTATCACCTTCTTTCCAGGTGCATATCATGA

Homology

[Close]

BLAST of CmoCh19G004630 vs. Swiss-Prot
Match: BAMO_GLYUR (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 7.5e-09
Identity = 40/78 (51.28%), Postives = 47/78 (60.26%), Query Frame = 1


Query: 1  METINVCIIIGFLLGVYMF----VRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSF 60
          ME   VC+    LL  Y+F    VR LN  +Y VKL RK +  LPPGDMGWPL+G   SF
Sbjct: 1  MEVHWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEHP-LPPGDMGWPLIGDLLSF 60

Query: 61 YKAFIVGGDPDSFIYHLL 75
           K F   G PDSFI +L+
Sbjct: 61 IKDF-SSGHPDSFINNLV 76

[Close]

BLAST of CmoCh19G004630 vs. Swiss-Prot
Match: KAO2_ARATH (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana GN=KAO2 PE=2 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 9.2e-07
Identity = 32/70 (45.71%), Postives = 46/70 (65.71%), Query Frame = 1


Query: 8  IIIGFLLGVYMFVRKLNEIW-YLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFIVGGDP 67
          II+G  L V  +V K   +W Y+ KLG K +  LPPGD+GWP++G+ +SF +AF    DP
Sbjct: 14 IILG--LFVLKWVLKRVNVWIYVSKLGEKKHY-LPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 68 DSFIYHLLSR 77
          +SFI   ++R
Sbjct: 74 ESFIQSYITR 79

[Close]

BLAST of CmoCh19G004630 vs. TrEMBL
Match: A0A0A0LI74_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G904060 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 2.5e-27
Identity = 57/73 (78.08%), Postives = 67/73 (91.78%), Query Frame = 1


Query: 4  INVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFIVG 63
          ++VC+IIG +LGVY FVRKLNE+WYLVKLGR+ YKSLP GD+GWPL+GSSFSFYKAF  G
Sbjct: 2  LSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAG 61

Query: 64 GDPDSFIYHLLSR 77
          GDPDSFI+HL+SR
Sbjct: 62 GDPDSFIHHLISR 74

[Close]

BLAST of CmoCh19G004630 vs. TrEMBL
Match: A0A0A0LF52_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G903530 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 4.9e-23
Identity = 53/76 (69.74%), Postives = 63/76 (82.89%), Query Frame = 1


Query: 1  METINVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60
          ME +NVC+IIG +LG Y+FVR+ NEIWYL KLGRK  KSLPPGD+GWP +GS+FSFYKAF
Sbjct: 1  MELMNVCVIIGVVLGFYVFVRRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKAF 60

Query: 61 IVGGDPDSFIYHLLSR 77
           V GDP +FI+ LL R
Sbjct: 61 KVEGDPYTFIHTLLLR 76

[Close]

BLAST of CmoCh19G004630 vs. TrEMBL
Match: A0A097IYP6_CUCSA (Cytochrome P450 OS=Cucumis sativus GN=Csa3G903550 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 2.0e-21
Identity = 52/76 (68.42%), Postives = 62/76 (81.58%), Query Frame = 1


Query: 1  METINVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60
          ME I+VC+IIG +LGV + VR+LNE+WYLVKLG +AYKSLPPGD+GWP++G SFS YK F
Sbjct: 1  MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60

Query: 61 IVGGDPDSFIYHLLSR 77
          IV  DP SFI  L SR
Sbjct: 61 IVQEDPISFIQSLHSR 76

[Close]

BLAST of CmoCh19G004630 vs. TrEMBL
Match: A0A0A0LHC2_CUCSA (Cytochrome P450 OS=Cucumis sativus GN=Csa3G9038540 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.8e-17
Identity = 47/73 (64.38%), Postives = 58/73 (79.45%), Query Frame = 1


Query: 4  INVCIIIGFLLGV-YMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFIV 63
          +NVC+IIG +LG  Y+F R+ NEIWY  KLGRK YKSLPPGD+GWP +GS+ +F KAF V
Sbjct: 5  MNVCVIIGVVLGFFYVFARRFNEIWYSFKLGRKIYKSLPPGDLGWPFVGSTLAFNKAFRV 64

Query: 64 GGDPDSFIY-HLL 75
           GDP +FI+ HLL
Sbjct: 65 VGDPYTFIHTHLL 77

[Close]

BLAST of CmoCh19G004630 vs. TrEMBL
Match: A0A059CHU0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02177 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 2.1e-10
Identity = 42/80 (52.50%), Postives = 51/80 (63.75%), Query Frame = 1


Query: 1  METINVCIIIGFLLGVYMFV----RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSF 60
          ME   +C+I    +G Y FV    RK+NE  Y+ KLG K Y SLPPGDMGWP LG   SF
Sbjct: 1  MEVNLMCLIFTCFVGGYAFVFGFLRKINEWLYVRKLGEKQY-SLPPGDMGWPFLGKMLSF 60

Query: 61 YKAFIVGGDPDSFIYHLLSR 77
          ++AF   GDPDSFI   ++R
Sbjct: 61 WRAFQF-GDPDSFISSFVTR 78

[Close]

BLAST of CmoCh19G004630 vs. TAIR10
Match: AT2G32440.1 (AT2G32440.1 ent-kaurenoic acid hydroxylase 2)

HSP 1 Score: 53.9 bits (128), Expect = 5.2e-08
Identity = 32/70 (45.71%), Postives = 46/70 (65.71%), Query Frame = 1


Query: 8  IIIGFLLGVYMFVRKLNEIW-YLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFIVGGDP 67
          II+G  L V  +V K   +W Y+ KLG K +  LPPGD+GWP++G+ +SF +AF    DP
Sbjct: 14 IILG--LFVLKWVLKRVNVWIYVSKLGEKKHY-LPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 68 DSFIYHLLSR 77
          +SFI   ++R
Sbjct: 74 ESFIQSYITR 79

[Close]

BLAST of CmoCh19G004630 vs. NCBI nr
Match: gi|778688019|ref|XP_011652661.1| (PREDICTED: beta-amyrin 11-oxidase-like [Cucumis sativus])

HSP 1 Score: 132.9 bits (333), Expect = 2.5e-28
Identity = 59/76 (77.63%), Postives = 69/76 (90.79%), Query Frame = 1


Query: 1  METINVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60
          ME ++VC+IIG +LGVY FVRKLNE+WYLVKLGR+ YKSLP GD+GWPL+GSSFSFYKAF
Sbjct: 2  MEMLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAF 61

Query: 61 IVGGDPDSFIYHLLSR 77
            GGDPDSFI+HL+SR
Sbjct: 62 KAGGDPDSFIHHLISR 77

[Close]

BLAST of CmoCh19G004630 vs. NCBI nr
Match: gi|700205284|gb|KGN60417.1| (hypothetical protein Csa_3G904060 [Cucumis sativus])

HSP 1 Score: 129.0 bits (323), Expect = 3.6e-27
Identity = 57/73 (78.08%), Postives = 67/73 (91.78%), Query Frame = 1


Query: 4  INVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAFIVG 63
          ++VC+IIG +LGVY FVRKLNE+WYLVKLGR+ YKSLP GD+GWPL+GSSFSFYKAF  G
Sbjct: 2  LSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKAFKAG 61

Query: 64 GDPDSFIYHLLSR 77
          GDPDSFI+HL+SR
Sbjct: 62 GDPDSFIHHLISR 74

[Close]

BLAST of CmoCh19G004630 vs. NCBI nr
Match: gi|778688013|ref|XP_011652660.1| (PREDICTED: beta-amyrin 11-oxidase-like [Cucumis sativus])

HSP 1 Score: 114.8 bits (286), Expect = 7.0e-23
Identity = 53/76 (69.74%), Postives = 63/76 (82.89%), Query Frame = 1


Query: 1  METINVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60
          ME +NVC+IIG +LG Y+FVR+ NEIWYL KLGRK  KSLPPGD+GWP +GS+FSFYKAF
Sbjct: 1  MELMNVCVIIGVVLGFYVFVRRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKAF 60

Query: 61 IVGGDPDSFIYHLLSR 77
           V GDP +FI+ LL R
Sbjct: 61 KVEGDPYTFIHTLLLR 76

[Close]

BLAST of CmoCh19G004630 vs. NCBI nr
Match: gi|700205280|gb|KGN60413.1| (hypothetical protein Csa_3G903530 [Cucumis sativus])

HSP 1 Score: 114.8 bits (286), Expect = 7.0e-23
Identity = 53/76 (69.74%), Postives = 63/76 (82.89%), Query Frame = 1


Query: 1  METINVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60
          ME +NVC+IIG +LG Y+FVR+ NEIWYL KLGRK  KSLPPGD+GWP +GS+FSFYKAF
Sbjct: 1  MELMNVCVIIGVVLGFYVFVRRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKAF 60

Query: 61 IVGGDPDSFIYHLLSR 77
           V GDP +FI+ LL R
Sbjct: 61 KVEGDPYTFIHTLLLR 76

[Close]

BLAST of CmoCh19G004630 vs. NCBI nr
Match: gi|659134177|ref|XP_008467067.1| (PREDICTED: ent-kaurenoic acid oxidase 2-like, partial [Cucumis melo])

HSP 1 Score: 109.8 bits (273), Expect = 2.3e-21
Identity = 53/77 (68.83%), Postives = 64/77 (83.12%), Query Frame = 1


Query: 1  METINVCIIIGFLLGVYMFV-RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKA 60
          ME I+VC+IIG +LGV + V R+LNE+WYLVKLG +AYKSLPPGD+GWP++GSSFSFYK 
Sbjct: 1  MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60

Query: 61 FIVGGDPDSFIYHLLSR 77
          F+V  DP SFI  L SR
Sbjct: 61 FVVQEDPISFIQSLHSR 77

The following BLAST results are available for this feature:

BLAST of CmoCh19G004630 vs. Swiss-Prot


Match Name	E-value	Identity	Description
BAMO_GLYUR	7.5e-09	51.28	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis GN=CYP88D6 PE=1 SV=1	[more]
KAO2_ARATH	9.2e-07	45.71	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana GN=KAO2 PE=2 SV=2	[more]

BLAST of CmoCh19G004630 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LI74_CUCSA	2.5e-27	78.08	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G904060 PE=4 SV=1	[more]
A0A0A0LF52_CUCSA	4.9e-23	69.74	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G903530 PE=4 SV=1	[more]
A0A097IYP6_CUCSA	2.0e-21	68.42	Cytochrome P450 OS=Cucumis sativus GN=Csa3G903550 PE=2 SV=1	[more]
A0A0A0LHC2_CUCSA	1.8e-17	64.38	Cytochrome P450 OS=Cucumis sativus GN=Csa3G9038540 PE=2 SV=1	[more]
A0A059CHU0_EUCGR	2.1e-10	52.50	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02177 PE=3 SV=1	[more]

BLAST of CmoCh19G004630 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G32440.1	5.2e-08	45.71	ent-kaurenoic acid hydroxylase 2	[more]

BLAST of CmoCh19G004630 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778688019\|ref\|XP_011652661.1\|	2.5e-28	77.63	PREDICTED: beta-amyrin 11-oxidase-like [Cucumis sativus]	[more]
gi\|700205284\|gb\|KGN60417.1\|	3.6e-27	78.08	hypothetical protein Csa_3G904060 [Cucumis sativus]	[more]
gi\|778688013\|ref\|XP_011652660.1\|	7.0e-23	69.74	PREDICTED: beta-amyrin 11-oxidase-like [Cucumis sativus]	[more]
gi\|700205280\|gb\|KGN60413.1\|	7.0e-23	69.74	hypothetical protein Csa_3G903530 [Cucumis sativus]	[more]
gi\|659134177\|ref\|XP_008467067.1\|	2.3e-21	68.83	PREDICTED: ent-kaurenoic acid oxidase 2-like, partial [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh19G004630.1	CmoCh19G004630.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR24286	FAMILY NOT NAMED	coord: 9..76 score: 6.9
None	No IPR available	PANTHER	PTHR24286:SF67	ENT-KAURENOIC ACID OXIDASE 1-RELATED	coord: 9..76 score: 6.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh19G004630	MELO3C031942.2	Melon (DHL92) v3.6.1	cmomedB548
CmoCh19G004630	CsaV3_3G001960	Cucumber (Chinese Long) v3	cmocucB0609

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh19G004630	Cucurbita maxima (Rimu)	cmacmoB508
CmoCh19G004630	Cucurbita maxima (Rimu)	cmacmoB597
CmoCh19G004630	Wild cucumber (PI 183967)	cmocpiB511
CmoCh19G004630	Cucumber (Chinese Long) v2	cmocuB505
CmoCh19G004630	Melon (DHL92) v3.5.1	cmomeB458
CmoCh19G004630	Watermelon (Charleston Gray)	cmowcgB446
CmoCh19G004630	Watermelon (97103) v1	cmowmB469
CmoCh19G004630	Cucurbita pepo (Zucchini)	cmocpeB481
CmoCh19G004630	Cucurbita pepo (Zucchini)	cmocpeB506
CmoCh19G004630	Bottle gourd (USVL1VR-Ls)	cmolsiB472
CmoCh19G004630	Bottle gourd (USVL1VR-Ls)	cmolsiB477
CmoCh19G004630	Cucumber (Gy14) v2	cgybcmoB354
CmoCh19G004630	Melon (DHL92) v3.6.1	cmomedB529
CmoCh19G004630	Silver-seed gourd	carcmoB0125
CmoCh19G004630	Silver-seed gourd	carcmoB1054
CmoCh19G004630	Cucumber (Chinese Long) v3	cmocucB0589
CmoCh19G004630	Cucumber (Chinese Long) v3	cmocucB0605
CmoCh19G004630	Watermelon (97103) v2	cmowmbB534
CmoCh19G004630	Wax gourd	cmowgoB0647
CmoCh19G004630	Wax gourd	cmowgoB0669
CmoCh19G004630	Cucurbita moschata (Rifu)	cmocmoB385
CmoCh19G004630	Cucumber (Gy14) v1	cgycmoB0868