CmoCh19G000740 (gene) Cucurbita moschata (Rifu)

NameCmoCh19G000740
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionPumpkin fruit trypsin inhibitor
LocationCmo_Chr19 : 410855 .. 411173 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAACAATGTCAGGTAGTAATTTTTAATGCAATGATGTATTGATATTAGTTCAATATGTATTGATATCATTTTATGTATCTAATAAGTATAGATTATATGGTGAAGTTATGTTGAATGAGGAGAAGTGGCGTTGGCCAGAGTTGGTGGGGATGGATGGGGAGGAAGCAATGAAGATAATACAGCAAGAGAATCCTTCTCTCGACGTAATTCTAATGCCACGAGGTCAAAACTGGGCATTTCCCAACTTTGTGTTCAATCGAGTCCGAGTCTTTGTTGACCATTGTGGAAAGGTGAATTCGACCCCTCGAATTGGCTAA

mRNA sequence

GAAAACAATGTCAGTTATGTTGAATGAGGAGAAGTGGCGTTGGCCAGAGTTGGTGGGGATGGATGGGGAGGAAGCAATGAAGATAATACAGCAAGAGAATCCTTCTCTCGACGTAATTCTAATGCCACGAGGTCAAAACTGGGCATTTCCCAACTTTGTGTTCAATCGAGTCCGAGTCTTTGTTGACCATTGTGGAAAGGTGAATTCGACCCCTCGAATTGGCTAA

Coding sequence (CDS)

ATGTCAGTTATGTTGAATGAGGAGAAGTGGCGTTGGCCAGAGTTGGTGGGGATGGATGGGGAGGAAGCAATGAAGATAATACAGCAAGAGAATCCTTCTCTCGACGTAATTCTAATGCCACGAGGTCAAAACTGGGCATTTCCCAACTTTGTGTTCAATCGAGTCCGAGTCTTTGTTGACCATTGTGGAAAGGTGAATTCGACCCCTCGAATTGGCTAA
BLAST of CmoCh19G000740 vs. Swiss-Prot
Match: ICI2_HORVU (Subtilisin-chymotrypsin inhibitor-2A OS=Hordeum vulgare PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 2.2e-07
Identity = 26/64 (40.62%), Postives = 36/64 (56.25%), Query Frame = 1

Query: 9  KWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNST 68
          K  WPELVG   EEA K+I Q+ P   +I++P G       +  +RVR+FVD    +   
Sbjct: 22 KTEWPELVGKSVEEAKKVILQDKPEAQIIVLPVG-TIVTMEYRIDRVRLFVDKLDNIAQV 81

Query: 69 PRIG 73
          PR+G
Sbjct: 82 PRVG 84

BLAST of CmoCh19G000740 vs. Swiss-Prot
Match: ICIW_WHEAT (Subtilisin-chymotrypsin inhibitor WSCI OS=Triticum aestivum PE=1 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 2.9e-07
Identity = 25/67 (37.31%), Postives = 38/67 (56.72%), Query Frame = 1

Query: 6  NEEKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKV 65
          + +K  WPELVG   EEA K+I Q+     ++++P G       +  +RVR+FVD   K+
Sbjct: 19 HNQKTEWPELVGKSVEEAKKVILQDKSEAQIVVLPVG-TIVTMEYRIDRVRLFVDSLDKI 78

Query: 66 NSTPRIG 73
             PR+G
Sbjct: 79 AQVPRVG 84

BLAST of CmoCh19G000740 vs. Swiss-Prot
Match: ICI3_HORVU (Subtilisin-chymotrypsin inhibitor-2B (Fragment) OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.1e-06
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 1

Query: 7  EEKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVN 66
           +K  WPELV    EEA K+I Q+ P   +I++P G       +  +RVR+FVD    + 
Sbjct: 8  NQKTEWPELVEKSVEEAKKVILQDKPEAQIIVLPVG-TIVTMEYRIDRVRLFVDRLDNIA 67

Query: 67 STPRIG 73
            PR+G
Sbjct: 68 QVPRVG 72

BLAST of CmoCh19G000740 vs. Swiss-Prot
Match: ITH5_CUCMA (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.4e-06
Identity = 26/64 (40.62%), Postives = 39/64 (60.94%), Query Frame = 1

Query: 9  KWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNST 68
          K  WP LVG+ G  A  II+++NP++  +++  G      +F  NRVR++V+  G V S 
Sbjct: 6  KSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTP-VTKDFRCNRVRIWVNKRGLVVSP 65

Query: 69 PRIG 73
          PRIG
Sbjct: 66 PRIG 68

BLAST of CmoCh19G000740 vs. Swiss-Prot
Match: HPI_HEVBR (Protease inhibitor HPI OS=Hevea brasiliensis GN=PI1 PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 3.2e-06
Identity = 28/61 (45.90%), Postives = 35/61 (57.38%), Query Frame = 1

Query: 12 WPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNSTPRI 71
          WPELVG +G+ A  IIQ EN ++  I++  G      +  FNRVRVFVD    V   P I
Sbjct: 11 WPELVGTNGDIAAGIIQTENANVKAIVVKEGLP-ITQDLNFNRVRVFVDENRVVTQVPAI 70

Query: 72 G 73
          G
Sbjct: 71 G 70

BLAST of CmoCh19G000740 vs. TrEMBL
Match: Q43421_CUCMA (Pumpkin fruit trypsin inhibitor OS=Cucurbita maxima GN=pfiAF4 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 9.3e-21
Identity = 51/65 (78.46%), Postives = 54/65 (83.08%), Query Frame = 1

Query: 8  EKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNS 67
          EK  WPELVG DGEEA+KIIQQENPSLDVILMPRGQNWA  ++  NRVRVF D  GKVNS
Sbjct: 3  EKLSWPELVGKDGEEAVKIIQQENPSLDVILMPRGQNWATKDYRPNRVRVFNDDSGKVNS 62

Query: 68 TPRIG 73
           PRIG
Sbjct: 63 IPRIG 67

BLAST of CmoCh19G000740 vs. TrEMBL
Match: Q6DKU8_CUCMA (Putative chymotrypsin protease inhibitor OS=Cucurbita maxima PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.6e-20
Identity = 51/65 (78.46%), Postives = 54/65 (83.08%), Query Frame = 1

Query: 8  EKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNS 67
          EK  WPELVG DGEEA+KIIQQENPSLDVILMPRGQNWA  ++  NRVRVF D  GKVNS
Sbjct: 3  EKSSWPELVGKDGEEAVKIIQQENPSLDVILMPRGQNWATLDYRPNRVRVFNDESGKVNS 62

Query: 68 TPRIG 73
           PRIG
Sbjct: 63 IPRIG 67

BLAST of CmoCh19G000740 vs. TrEMBL
Match: Q43420_CUCMA (Pumpkin Fruit Chymotrypsin Inhibitor OS=Cucurbita maxima GN=pfiBM7 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 7.8e-20
Identity = 51/65 (78.46%), Postives = 53/65 (81.54%), Query Frame = 1

Query: 8  EKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNS 67
          EK  WPELVG DGEEA+KIIQQENPSLDVILMPRGQNWA  +   NRVRVF D  GKVNS
Sbjct: 3  EKSSWPELVGEDGEEAVKIIQQENPSLDVILMPRGQNWATLDCRPNRVRVFNDESGKVNS 62

Query: 68 TPRIG 73
           PRIG
Sbjct: 63 IPRIG 67

BLAST of CmoCh19G000740 vs. TrEMBL
Match: A0A0K9RNA1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_047460 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.1e-08
Identity = 35/65 (53.85%), Postives = 45/65 (69.23%), Query Frame = 1

Query: 9  KWRWPELVGMDGEEAMKIIQQENPSL-DVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNS 68
          K  WPELVG DG EA  II+ EN S+ +V+++P G      ++  +RVRVFVD CGKV+S
Sbjct: 15 KKSWPELVGKDGNEAAGIIKSENCSVKNVVVLPEGSP-VTKDYRTDRVRVFVDTCGKVSS 74

Query: 69 TPRIG 73
          TP IG
Sbjct: 75 TPYIG 78

BLAST of CmoCh19G000740 vs. TrEMBL
Match: A0A161XY83_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_010421 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.4e-08
Identity = 30/67 (44.78%), Postives = 46/67 (68.66%), Query Frame = 1

Query: 6  NEEKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKV 65
          N  +  WPE+VGM GEEA K I++E+P + V ++P+  ++   +F  NRVRV+VD  GKV
Sbjct: 27 NRGRKTWPEVVGMTGEEAKKQIEEESPGISVHIVPQ-NSFVTMDFRTNRVRVYVDSSGKV 86

Query: 66 NSTPRIG 73
           + P++G
Sbjct: 87 ANAPKVG 92

BLAST of CmoCh19G000740 vs. TAIR10
Match: AT5G43580.1 (AT5G43580.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 53.1 bits (126), Expect = 8.0e-08
Identity = 27/72 (37.50%), Postives = 43/72 (59.72%), Query Frame = 1

Query: 2  SVMLNEEKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPN-FVFNRVRVFVD 61
          S+  +  K  WPEL+G  GE+A ++I++ENP +  +++  G     P  F+ +RV V+V+
Sbjct: 30 SICEDPGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGT--VVPEIFICSRVYVWVN 89

Query: 62 HCGKVNSTPRIG 73
           CG V   P IG
Sbjct: 90 DCGIVVQIPIIG 99

BLAST of CmoCh19G000740 vs. TAIR10
Match: AT2G38900.2 (AT2G38900.2 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 47.4 bits (111), Expect = 4.4e-06
Identity = 24/61 (39.34%), Postives = 36/61 (59.02%), Query Frame = 1

Query: 12 WPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNSTPRI 71
          WPEL+G +G+ A  +I+ EN SL+V+++  G N+   +    RVRV+VD    V   P  
Sbjct: 29 WPELLGTNGDYAASVIKGENSSLNVVVVSDG-NYVTEDLSCYRVRVWVDEIRIVVRNPTA 88

Query: 72 G 73
          G
Sbjct: 89 G 88

BLAST of CmoCh19G000740 vs. TAIR10
Match: AT2G38870.1 (AT2G38870.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 47.4 bits (111), Expect = 4.4e-06
Identity = 24/61 (39.34%), Postives = 37/61 (60.66%), Query Frame = 1

Query: 12 WPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNSTPRI 71
          WPEL G +G+ A  +I++ENP+++  ++  G      +F  +RVRVFVD    V  TP+ 
Sbjct: 11 WPELTGTNGDYAAVVIERENPTVNAAVILDGSP-VTADFRCDRVRVFVDGNRIVVKTPKS 70

Query: 72 G 73
          G
Sbjct: 71 G 70

BLAST of CmoCh19G000740 vs. NCBI nr
Match: gi|887420|emb|CAA57307.1| (Pumpkin fruit trypsin inhibitor [Cucurbita maxima])

HSP 1 Score: 107.1 bits (266), Expect = 1.3e-20
Identity = 51/65 (78.46%), Postives = 54/65 (83.08%), Query Frame = 1

Query: 8  EKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNS 67
          EK  WPELVG DGEEA+KIIQQENPSLDVILMPRGQNWA  ++  NRVRVF D  GKVNS
Sbjct: 3  EKLSWPELVGKDGEEAVKIIQQENPSLDVILMPRGQNWATKDYRPNRVRVFNDDSGKVNS 62

Query: 68 TPRIG 73
           PRIG
Sbjct: 63 IPRIG 67

BLAST of CmoCh19G000740 vs. NCBI nr
Match: gi|50262213|gb|AAT72726.1| (putative chymotrypsin protease inhibitor [Cucurbita maxima])

HSP 1 Score: 106.3 bits (264), Expect = 2.3e-20
Identity = 51/65 (78.46%), Postives = 54/65 (83.08%), Query Frame = 1

Query: 8  EKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNS 67
          EK  WPELVG DGEEA+KIIQQENPSLDVILMPRGQNWA  ++  NRVRVF D  GKVNS
Sbjct: 3  EKSSWPELVGKDGEEAVKIIQQENPSLDVILMPRGQNWATLDYRPNRVRVFNDESGKVNS 62

Query: 68 TPRIG 73
           PRIG
Sbjct: 63 IPRIG 67

BLAST of CmoCh19G000740 vs. NCBI nr
Match: gi|887418|emb|CAA57203.1| (Pumpkin Fruit Chymotrypsin Inhibitor [Cucurbita maxima])

HSP 1 Score: 104.0 bits (258), Expect = 1.1e-19
Identity = 51/65 (78.46%), Postives = 53/65 (81.54%), Query Frame = 1

Query: 8  EKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNS 67
          EK  WPELVG DGEEA+KIIQQENPSLDVILMPRGQNWA  +   NRVRVF D  GKVNS
Sbjct: 3  EKSSWPELVGEDGEEAVKIIQQENPSLDVILMPRGQNWATLDCRPNRVRVFNDESGKVNS 62

Query: 68 TPRIG 73
           PRIG
Sbjct: 63 IPRIG 67

BLAST of CmoCh19G000740 vs. NCBI nr
Match: gi|902228146|gb|KNA20995.1| (hypothetical protein SOVF_047460 [Spinacia oleracea])

HSP 1 Score: 67.0 bits (162), Expect = 1.5e-08
Identity = 35/65 (53.85%), Postives = 45/65 (69.23%), Query Frame = 1

Query: 9  KWRWPELVGMDGEEAMKIIQQENPSL-DVILMPRGQNWAFPNFVFNRVRVFVDHCGKVNS 68
          K  WPELVG DG EA  II+ EN S+ +V+++P G      ++  +RVRVFVD CGKV+S
Sbjct: 15 KKSWPELVGKDGNEAAGIIKSENCSVKNVVVLPEGSP-VTKDYRTDRVRVFVDTCGKVSS 74

Query: 69 TPRIG 73
          TP IG
Sbjct: 75 TPYIG 78

BLAST of CmoCh19G000740 vs. NCBI nr
Match: gi|1021043886|gb|KZN01667.1| (hypothetical protein DCAR_010421 [Daucus carota subsp. sativus])

HSP 1 Score: 65.9 bits (159), Expect = 3.4e-08
Identity = 30/67 (44.78%), Postives = 46/67 (68.66%), Query Frame = 1

Query: 6  NEEKWRWPELVGMDGEEAMKIIQQENPSLDVILMPRGQNWAFPNFVFNRVRVFVDHCGKV 65
          N  +  WPE+VGM GEEA K I++E+P + V ++P+  ++   +F  NRVRV+VD  GKV
Sbjct: 27 NRGRKTWPEVVGMTGEEAKKQIEEESPGISVHIVPQ-NSFVTMDFRTNRVRVYVDSSGKV 86

Query: 66 NSTPRIG 73
           + P++G
Sbjct: 87 ANAPKVG 92

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ICI2_HORVU2.2e-0740.63Subtilisin-chymotrypsin inhibitor-2A OS=Hordeum vulgare PE=1 SV=2[more]
ICIW_WHEAT2.9e-0737.31Subtilisin-chymotrypsin inhibitor WSCI OS=Triticum aestivum PE=1 SV=2[more]
ICI3_HORVU1.1e-0637.88Subtilisin-chymotrypsin inhibitor-2B (Fragment) OS=Hordeum vulgare PE=2 SV=1[more]
ITH5_CUCMA1.4e-0640.63Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1[more]
HPI_HEVBR3.2e-0645.90Protease inhibitor HPI OS=Hevea brasiliensis GN=PI1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
Q43421_CUCMA9.3e-2178.46Pumpkin fruit trypsin inhibitor OS=Cucurbita maxima GN=pfiAF4 PE=2 SV=1[more]
Q6DKU8_CUCMA1.6e-2078.46Putative chymotrypsin protease inhibitor OS=Cucurbita maxima PE=4 SV=1[more]
Q43420_CUCMA7.8e-2078.46Pumpkin Fruit Chymotrypsin Inhibitor OS=Cucurbita maxima GN=pfiBM7 PE=2 SV=1[more]
A0A0K9RNA1_SPIOL1.1e-0853.85Uncharacterized protein OS=Spinacia oleracea GN=SOVF_047460 PE=4 SV=1[more]
A0A161XY83_DAUCA2.4e-0844.78Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_010421 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G43580.18.0e-0837.50 Serine protease inhibitor, potato inhibitor I-type family protein[more]
AT2G38900.24.4e-0639.34 Serine protease inhibitor, potato inhibitor I-type family protein[more]
AT2G38870.14.4e-0639.34 Serine protease inhibitor, potato inhibitor I-type family protein[more]
Match NameE-valueIdentityDescription
gi|887420|emb|CAA57307.1|1.3e-2078.46Pumpkin fruit trypsin inhibitor [Cucurbita maxima][more]
gi|50262213|gb|AAT72726.1|2.3e-2078.46putative chymotrypsin protease inhibitor [Cucurbita maxima][more]
gi|887418|emb|CAA57203.1|1.1e-1978.46Pumpkin Fruit Chymotrypsin Inhibitor [Cucurbita maxima][more]
gi|902228146|gb|KNA20995.1|1.5e-0853.85hypothetical protein SOVF_047460 [Spinacia oleracea][more]
gi|1021043886|gb|KZN01667.1|3.4e-0844.78hypothetical protein DCAR_010421 [Daucus carota subsp. sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000864Prot_inh_pot1
Vocabulary: Molecular Function
TermDefinition
GO:0004867serine-type endopeptidase inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0009611response to wounding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0009611 response to wounding
cellular_component GO:0005575 cellular_component
molecular_function GO:0008233 peptidase activity
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh19G000740.1CmoCh19G000740.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRODOMPD002604coord: 12..72
score: 1.0
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 9..72
score: 8.9
IPR000864Proteinase inhibitor I13, potato inhibitor IPROSITEPS00285POTATO_INHIBITORcoord: 12..23
scor
IPR000864Proteinase inhibitor I13, potato inhibitor IunknownSSF54654CI-2 family of serine protease inhibitorscoord: 5..72
score: 1.26
NoneNo IPR availableGENE3DG3DSA:3.30.10.10coord: 7..72
score: 8.4
NoneNo IPR availablePANTHERPTHR33091FAMILY NOT NAMEDcoord: 7..72
score: 1.6

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None