CmoCh17G005950 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh17G005950
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
Description(Ribosome inactivating protein) (3.2.2.22)
LocationCmo_Chr17 : 6320761 .. 6321603 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATAGACTCTCAGTGCTGTCTTTGCTAATTCTCACCATCTTCCATGAAGGCCACACTGCTGTAGGCGATAATCTCAGCTTCAGCTTGTCGGGTTCAAGTTCCAAATCTTACAGCAAATTCATTACATCTCTGAGGAACGCTCTTCCAAATGCTGGAAAGGTCTACAATATACCTCTATTACTTCCCAATCTCAAGGGCTCAGCACGCTACACACTAATGAAACTCTCCAACTATGAGGAGAAAACTATCACGGTGGCTATAGATGTAACCAACGTTTACATTATGGGGTATCTTGTCAATACAACATCCTACTTTTTCAACGAGCCCGCCGCTCAGTTAGCGTCTGAGTTCGTATTCCAAGGTACTAAGCACATTCTTCTTCCATACTCTGGCAATTACCAAAAGCTTCAAATTGCTGCAGACAAGGATAGAGATTCAATTCCTCTGGGATTCCTTGCCTTGGACACTGCAATTTCCGCTTTGTATCATTATGACACCAAAGCTGCTCCGGCGGCATTTCTCGTACTCATTCAGACCACTGCCGAGGCTTCAAGATTTAAATACATTGAGAAACAAATTATCGATAGAATTGGCAGAAACGAGGTGCCAAGTTTAGCAGCCATAAGTTTGGAAAACGAATGGTCTCTTCTGTCCAAACAAATTCAGATAGCGAACTCCAACAATGGAACATTTCAAACTCCTGTCAAGCTTATTAATGATAAAGGCCTTTCAGTGGAGGTTACTAACGTTAGCTCTCTTGTTGTAACCAATAACATCAAGCTGCTGCTAAACAAGCAAAATGTTGCAACTATTAAGCCTGAAGCTATTACAATTCCTTAG

mRNA sequence

ATGAATAGACTCTCAGTGCTGTCTTTGCTAATTCTCACCATCTTCCATGAAGGCCACACTGCTGTAGGCGATAATCTCAGCTTCAGCTTGTCGGGTTCAAGTTCCAAATCTTACAGCAAATTCATTACATCTCTGAGGAACGCTCTTCCAAATGCTGGAAAGGTCTACAATATACCTCTATTACTTCCCAATCTCAAGGGCTCAGCACGCTACACACTAATGAAACTCTCCAACTATGAGGAGAAAACTATCACGGTGGCTATAGATGTAACCAACGTTTACATTATGGGGTATCTTGTCAATACAACATCCTACTTTTTCAACGAGCCCGCCGCTCAGTTAGCGTCTGAGTTCGTATTCCAAGGTACTAAGCACATTCTTCTTCCATACTCTGGCAATTACCAAAAGCTTCAAATTGCTGCAGACAAGGATAGAGATTCAATTCCTCTGGGATTCCTTGCCTTGGACACTGCAATTTCCGCTTTGTATCATTATGACACCAAAGCTGCTCCGGCGGCATTTCTCGTACTCATTCAGACCACTGCCGAGGCTTCAAGATTTAAATACATTGAGAAACAAATTATCGATAGAATTGGCAGAAACGAGGTGCCAAGTTTAGCAGCCATAAGTTTGGAAAACGAATGGTCTCTTCTGTCCAAACAAATTCAGATAGCGAACTCCAACAATGGAACATTTCAAACTCCTGTCAAGCTTATTAATGATAAAGGCCTTTCAGTGGAGGTTACTAACGTTAGCTCTCTTGTTGTAACCAATAACATCAAGCTGCTGCTAAACAAGCAAAATGTTGCAACTATTAAGCCTGAAGCTATTACAATTCCTTAG

Coding sequence (CDS)

ATGAATAGACTCTCAGTGCTGTCTTTGCTAATTCTCACCATCTTCCATGAAGGCCACACTGCTGTAGGCGATAATCTCAGCTTCAGCTTGTCGGGTTCAAGTTCCAAATCTTACAGCAAATTCATTACATCTCTGAGGAACGCTCTTCCAAATGCTGGAAAGGTCTACAATATACCTCTATTACTTCCCAATCTCAAGGGCTCAGCACGCTACACACTAATGAAACTCTCCAACTATGAGGAGAAAACTATCACGGTGGCTATAGATGTAACCAACGTTTACATTATGGGGTATCTTGTCAATACAACATCCTACTTTTTCAACGAGCCCGCCGCTCAGTTAGCGTCTGAGTTCGTATTCCAAGGTACTAAGCACATTCTTCTTCCATACTCTGGCAATTACCAAAAGCTTCAAATTGCTGCAGACAAGGATAGAGATTCAATTCCTCTGGGATTCCTTGCCTTGGACACTGCAATTTCCGCTTTGTATCATTATGACACCAAAGCTGCTCCGGCGGCATTTCTCGTACTCATTCAGACCACTGCCGAGGCTTCAAGATTTAAATACATTGAGAAACAAATTATCGATAGAATTGGCAGAAACGAGGTGCCAAGTTTAGCAGCCATAAGTTTGGAAAACGAATGGTCTCTTCTGTCCAAACAAATTCAGATAGCGAACTCCAACAATGGAACATTTCAAACTCCTGTCAAGCTTATTAATGATAAAGGCCTTTCAGTGGAGGTTACTAACGTTAGCTCTCTTGTTGTAACCAATAACATCAAGCTGCTGCTAAACAAGCAAAATGTTGCAACTATTAAGCCTGAAGCTATTACAATTCCTTAG
Homology
[Close]
BLAST of CmoCh17G005950 vs. Swiss-Prot
Match: RIP1_CUCFI (Putative ribosome-inactivating protein OS=Cucumis ficifolius PE=2 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 1.5e-107
Identity = 208/280 (74.29%), Postives = 238/280 (85.00%), Query Frame = 1 

Query: 1   MNRLSVLS-LLILTIFHEGHTAVGD-NLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNI 60
           MNR SVL  L+IL+IFH   TA GD  + FSL GS+ KSYSKFITS+RNALPNAG +YNI
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNI 60

Query: 61  PLLLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEF 120
           PLL+P++ GS RY LM+LSNYE  TIT+A+DVTNVYIMGYLVN TSYFFNE  AQLAS+F
Sbjct: 61  PLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKF 120

Query: 121 VFQGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLI 180
           VFQGTK I LPYSGNYQKLQ  A K+RDSIPLGF+ALD+AIS LY+YD+++AP AFLVLI
Sbjct: 121 VFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLI 180

Query: 181 QTTAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKL 240
           QTTAEA+R+KYIEKQIIDRI  ++VP LAAISLENEWSLLSKQIQIA SNNG FQTPVK+
Sbjct: 181 QTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKI 240

Query: 241 INDKGLSVEVTNVSSLVVTNNIKLLLNKQNVATIKPEAIT 279
           INDKG+  EVTNVSSLVVT NI LLLNK N+A+ +   I+
Sbjct: 241 INDKGILTEVTNVSSLVVTKNIMLLLNKLNIASFEDHVIS 280

[Close]
BLAST of CmoCh17G005950 vs. Swiss-Prot
Match: RIPA_LUFAE (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.5e-91
Identity = 182/264 (68.94%), Postives = 216/264 (81.82%), Query Frame = 1 

Query: 8   SLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLLPNLKG 67
           ++LIL IF    T   D + FSLSGSSS SYSKFI  LR ALP+ G VYNI LLL +  G
Sbjct: 5   TVLILAIFVAASTVEAD-VRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSASG 64

Query: 68  SARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQGTKHIL 127
           ++RYTLM LSNY+ K ITVA+DVTNVYIMGYLVN+TSYFFNE  A+LAS++VF+G+  + 
Sbjct: 65  ASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVT 124

Query: 128 LPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTTAEASRF 187
           LPYSGNY+KLQ AA K R+ IPLGF ALD+AI+ L+HYD+ AA AAFLV+IQTTAEASRF
Sbjct: 125 LPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRF 184

Query: 188 KYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLINDKGLSVE 247
           KYIE QII+RI +N+VPSLA ISLENEWS LSKQIQ+A +NNGTF+TPV + +DKG  VE
Sbjct: 185 KYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVE 244

Query: 248 VTNVSSLVVTNNIKLLLN-KQNVA 271
           +TNV+S VVT NI+LLLN KQNVA
Sbjct: 245 ITNVTSKVVTKNIQLLLNYKQNVA 267

[Close]
BLAST of CmoCh17G005950 vs. Swiss-Prot
Match: RIP1_MOMCH (Ribosome-inactivating protein momordin I OS=Momordica charantia PE=1 SV=2)

HSP 1 Score: 327.8 bits (839), Expect = 1.2e-88
Identity = 176/271 (64.94%), Postives = 214/271 (78.97%), Query Frame = 1 

Query: 1   MNRLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPL 60
           M+R SVLS LIL IF  G    GD +SF LSG+  +SY  FI  LRNALP   KVYNIPL
Sbjct: 1   MSRFSVLSFLILAIFLGGSIVKGD-VSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVF 120
           LLP++ G+ RY LM L NY+ KTITVA+DVTNVYIMGYL +TTSYFFNEPAA+LAS++VF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 QGTKH-ILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQ 180
           +  +  I LPYSGNY++LQIAA K R+ IP+G  ALD+AIS L HYD+ AA  A LVLIQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 TTAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLI 240
           TTAEA+RFKYIE+QI +R  R+EVPSLA ISLEN WS LSKQIQ+A  NNG F+TP+ L+
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 NDKGLSVEVTNVSSLVVTNNIKLLLNKQNVA 271
           ++KG  V++TNV+S VVT+NI+LLLN +N+A
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIA 270

[Close]
BLAST of CmoCh17G005950 vs. Swiss-Prot
Match: RIP_LUFAC (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula PE=1 SV=2)

HSP 1 Score: 318.9 bits (816), Expect = 5.4e-86
Identity = 169/241 (70.12%), Postives = 200/241 (82.99%), Query Frame = 1 

Query: 25  NLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLLPNLKGSARYTLMKLSNYEEKTI 84
           ++SFSLSGSSS SYSKFI +LR ALP+ G VYNI LLL +  G++RYTLMKLSNY+ K I
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 85  TVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQGTKHILLPYSGNYQKLQIAADKD 144
           TVAIDVTNVYIMGYLVN+TSYFFNE  A+LAS++VF G+  + LPYSGNY+KLQ AA K 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 145 RDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTTAEASRFKYIEKQIIDRIGRNEVP 204
           R+ IPLGF ALD+AI+ L+HYD+ AA AAFLV+IQTTAE+SRFKYIE QII RI +N VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 205 SLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLINDKGLSVEVTNVSSLVVTNNIKLLL 264
           SLA ISLENEWS LSKQIQ+A +NNGTF+TPV +++  G  VE+ NV S VVT NI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 265 N 266
           N
Sbjct: 241 N 241

[Close]
BLAST of CmoCh17G005950 vs. Swiss-Prot
Match: RIPB_LUFAE (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 9.2e-86
Identity = 167/249 (67.07%), Postives = 210/249 (84.34%), Query Frame = 1 

Query: 25  NLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLLPNLKGSARYTLMKLSNYEEKTI 84
           N+SFSLSG+ SKSYSKFIT+LR ALP+  KV NIPLLLP+  G++RY LM+LSNY+ K I
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQGTKHILLPYSGNYQKLQIAADKD 144
           T+AIDVTNVYIMGYLVN+TSYF NE  A+LAS++VF+G+  + +PYSGNY++LQ AA K 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 145 RDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTTAEASRFKYIEKQIIDRIGRNEVP 204
           R+ IPLGF ALD+A+++++HYD+ AA AAFLV++QTTAEASRFKYIE QII+RI +NEVP
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 205 SLAAISLENE-WSLLSKQIQIANSNNGTFQTPVKLINDKGLSVEVTNVSSLVVTN--NIK 264
           S AA+SLENE WSLLSKQIQ+A +NNG F+TPV +I++KG  VE+TN++S V     N K
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

Query: 265 LLLNKQNVA 271
           LLLNKQN+A
Sbjct: 242 LLLNKQNIA 250

[Close]
BLAST of CmoCh17G005950 vs. TrEMBL
Match: Q00980_LUFAE (rRNA N-glycosidase OS=Luffa aegyptiaca PE=2 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 4.6e-92
Identity = 183/270 (67.78%), Postives = 224/270 (82.96%), Query Frame = 1 

Query: 1   MNRLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPL 60
           MNR + LSLLI+ I     T  G N+SFSLSG+ SKSYSKFIT+LR ALP+  KV NIPL
Sbjct: 1   MNRFTFLSLLIILIAF--FTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVF 120
           LLP+  G++RY LM+LSNY+ K IT+AIDVTNVYIMGYLVN+TSYFFNE  A+LAS++VF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 QGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQT 180
           +G+  + LPYSGNY++LQ AA K R+ IPLGF A D+AI++L+HYD+ AA  AFLV+IQT
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIN 240
           TAEASRFKYIE QII+RI +NEVPS AA+SLENEWS LSKQIQ+A +NNG F+TPV +I+
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DKGLSVEVTNVSSLVVTNNIKLLLNKQNVA 271
           +KG  VE+ +V+S VVTNNIKLLLNKQN+A
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]
BLAST of CmoCh17G005950 vs. TrEMBL
Match: B0EVM6_LUFAC (rRNA N-glycosidase OS=Luffa acutangula GN=RIPI PE=2 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 4.6e-92
Identity = 183/270 (67.78%), Postives = 224/270 (82.96%), Query Frame = 1 

Query: 1   MNRLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPL 60
           MNR + LSLLI+ I     T  G N+SFSLSG+ SKSYSKFIT+LR ALP+  KV NIPL
Sbjct: 1   MNRFTFLSLLIILIAF--FTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVF 120
           LLP+  G++RY LM+LSNY+ K IT+AIDVTNVYIMGYLVN+TSYFFNE  A+LAS++VF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 QGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQT 180
           +G+  + LPYSGNY++LQ AA K R+ IPLGF A D+AI++L+HYD+ AA  AFLV+IQT
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIN 240
           TAEASRFKYIE QII+RI +NEVPS AA+SLENEWS LSKQIQ+A +NNG F+TPV +I+
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DKGLSVEVTNVSSLVVTNNIKLLLNKQNVA 271
           +KG  VE+ +V+S VVTNNIKLLLNKQN+A
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]
BLAST of CmoCh17G005950 vs. TrEMBL
Match: S6CNX5_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 9.9e-87
Identity = 176/269 (65.43%), Postives = 213/269 (79.18%), Query Frame = 1 

Query: 3   RLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLL 62
           R SVLS LIL IF  G    GD +SF LSG+  +SY  FI  LRNALP   KVYNIPLLL
Sbjct: 1   RFSVLSFLILAIFLGGSIVKGD-VSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLL 60

Query: 63  PNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQG 122
           P++ G+ RY LM L NY+ KTITVA+DVTNVYIMGYL +TTSYFFNEPAA+LAS++VF+ 
Sbjct: 61  PSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRD 120

Query: 123 TKH-ILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTT 182
            +  I LPYSGNY++LQIAA K R+ IP+G LALD+AIS L HYD+ AA  A LVLIQTT
Sbjct: 121 ARRKITLPYSGNYERLQIAAGKPREKIPIGLLALDSAISTLLHYDSTAAAGALLVLIQTT 180

Query: 183 AEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIND 242
           AEA+RFKYIE+QI +R  R+EVPSLA ISLEN WS LSKQIQ+A  NNG F+TP+ L+++
Sbjct: 181 AEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDN 240

Query: 243 KGLSVEVTNVSSLVVTNNIKLLLNKQNVA 271
           KG  V++TNV+S VVT+NI+LLLN +N+A
Sbjct: 241 KGNRVQITNVTSKVVTSNIQLLLNTRNIA 268

[Close]
BLAST of CmoCh17G005950 vs. TrEMBL
Match: S6CNH1_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.2e-86
Identity = 176/269 (65.43%), Postives = 212/269 (78.81%), Query Frame = 1 

Query: 3   RLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLL 62
           R SVLS LIL IF  G    GD +SF LSG+  +SY  FI  LRNALP   KVYNIPLLL
Sbjct: 1   RFSVLSFLILAIFLGGSIVKGD-VSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLL 60

Query: 63  PNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQG 122
           P++ G+ RY LM L NY+ KTITVA+DVTNVYIMGYL +TTSYFFNEPAA+LAS++VFQ 
Sbjct: 61  PSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFQD 120

Query: 123 TKH-ILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTT 182
            +  I LPYSGNY++LQIAA K R+ IP+G  ALD+AIS L HYD+ AA  A LVLIQTT
Sbjct: 121 ARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQTT 180

Query: 183 AEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIND 242
           AEA+RFKYIE+QI +R  R+EVPSLA ISLEN WS LSKQIQ+A  NNG F+TP+ L+++
Sbjct: 181 AEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDN 240

Query: 243 KGLSVEVTNVSSLVVTNNIKLLLNKQNVA 271
           KG  V++TNV+S VVT+NI+LLLN +N+A
Sbjct: 241 KGNRVQITNVTSKVVTSNIQLLLNTRNIA 268

[Close]
BLAST of CmoCh17G005950 vs. TrEMBL
Match: S6CNX9_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 4.9e-86
Identity = 175/269 (65.06%), Postives = 212/269 (78.81%), Query Frame = 1 

Query: 3   RLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLL 62
           R SVLS LIL IF  G    GD +SF LSG+  +SY  FI  LRNALP   KVYNIPLLL
Sbjct: 1   RFSVLSFLILAIFLGGSIVKGD-VSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLL 60

Query: 63  PNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQG 122
           P++ G+ RY LM L NY+ KTITVA+DVTNVYIMGYL +TTSYFFNEPAA+LAS++VF+ 
Sbjct: 61  PSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRD 120

Query: 123 TKH-ILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTT 182
            +  I LPYSGNY++LQIAA K R+ IP+G  ALD+AIS L HYD+ AA  A LVLIQTT
Sbjct: 121 ARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQTT 180

Query: 183 AEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIND 242
           AEA+RFKYIE+QI +R  R+EVPSLA ISLEN WS LSKQIQ+A  NNG F+TP+ L+++
Sbjct: 181 AEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDN 240

Query: 243 KGLSVEVTNVSSLVVTNNIKLLLNKQNVA 271
           KG  V++TNV+S VVT+NI+LLLN +N+A
Sbjct: 241 KGSRVQITNVTSKVVTSNIQLLLNTRNIA 268

[Close]
BLAST of CmoCh17G005950 vs. NCBI nr
Match: gi|14195121|sp|Q9FRX4.1|RIP1_CUCFI (RecName: Full=Putative ribosome-inactivating protein; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 390.6 bits (1002), Expect = 2.3e-105
Identity = 208/280 (74.29%), Postives = 238/280 (85.00%), Query Frame = 1 

Query: 1   MNRLSVLS-LLILTIFHEGHTAVGD-NLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNI 60
           MNR SVL  L+IL+IFH   TA GD  + FSL GS+ KSYSKFITS+RNALPNAG +YNI
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNI 60

Query: 61  PLLLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEF 120
           PLL+P++ GS RY LM+LSNYE  TIT+A+DVTNVYIMGYLVN TSYFFNE  AQLAS+F
Sbjct: 61  PLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKF 120

Query: 121 VFQGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLI 180
           VFQGTK I LPYSGNYQKLQ  A K+RDSIPLGF+ALD+AIS LY+YD+++AP AFLVLI
Sbjct: 121 VFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLI 180

Query: 181 QTTAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKL 240
           QTTAEA+R+KYIEKQIIDRI  ++VP LAAISLENEWSLLSKQIQIA SNNG FQTPVK+
Sbjct: 181 QTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKI 240

Query: 241 INDKGLSVEVTNVSSLVVTNNIKLLLNKQNVATIKPEAIT 279
           INDKG+  EVTNVSSLVVT NI LLLNK N+A+ +   I+
Sbjct: 241 INDKGILTEVTNVSSLVVTKNIMLLLNKLNIASFEDHVIS 280

[Close]
BLAST of CmoCh17G005950 vs. NCBI nr
Match: gi|19150|emb|CAA44230.1| (b-luffin [Luffa aegyptiaca])

HSP 1 Score: 345.9 bits (886), Expect = 6.6e-92
Identity = 183/270 (67.78%), Postives = 224/270 (82.96%), Query Frame = 1 

Query: 1   MNRLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPL 60
           MNR + LSLLI+ I     T  G N+SFSLSG+ SKSYSKFIT+LR ALP+  KV NIPL
Sbjct: 1   MNRFTFLSLLIILIAF--FTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVF 120
           LLP+  G++RY LM+LSNY+ K IT+AIDVTNVYIMGYLVN+TSYFFNE  A+LAS++VF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 QGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQT 180
           +G+  + LPYSGNY++LQ AA K R+ IPLGF A D+AI++L+HYD+ AA  AFLV+IQT
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIN 240
           TAEASRFKYIE QII+RI +NEVPS AA+SLENEWS LSKQIQ+A +NNG F+TPV +I+
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DKGLSVEVTNVSSLVVTNNIKLLLNKQNVA 271
           +KG  VE+ +V+S VVTNNIKLLLNKQN+A
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]
BLAST of CmoCh17G005950 vs. NCBI nr
Match: gi|132590|sp|Q00465.1|RIPA_LUFAE (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 337.4 bits (864), Expect = 2.4e-89
Identity = 182/264 (68.94%), Postives = 216/264 (81.82%), Query Frame = 1 

Query: 8   SLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLLPNLKG 67
           ++LIL IF    T   D + FSLSGSSS SYSKFI  LR ALP+ G VYNI LLL +  G
Sbjct: 5   TVLILAIFVAASTVEAD-VRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSASG 64

Query: 68  SARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQGTKHIL 127
           ++RYTLM LSNY+ K ITVA+DVTNVYIMGYLVN+TSYFFNE  A+LAS++VF+G+  + 
Sbjct: 65  ASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVT 124

Query: 128 LPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTTAEASRF 187
           LPYSGNY+KLQ AA K R+ IPLGF ALD+AI+ L+HYD+ AA AAFLV+IQTTAEASRF
Sbjct: 125 LPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRF 184

Query: 188 KYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLINDKGLSVE 247
           KYIE QII+RI +N+VPSLA ISLENEWS LSKQIQ+A +NNGTF+TPV + +DKG  VE
Sbjct: 185 KYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVE 244

Query: 248 VTNVSSLVVTNNIKLLLN-KQNVA 271
           +TNV+S VVT NI+LLLN KQNVA
Sbjct: 245 ITNVTSKVVTKNIQLLLNYKQNVA 267

[Close]
BLAST of CmoCh17G005950 vs. NCBI nr
Match: gi|34810785|pdb|1NIO|A (Chain A, Crystal Structure Of Beta-Luffin, A Ribosome Inactivating Protein At 2.0a Resolution)

HSP 1 Score: 334.0 bits (855), Expect = 2.6e-88
Identity = 172/246 (69.92%), Postives = 210/246 (85.37%), Query Frame = 1 

Query: 25  NLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLLPNLKGSARYTLMKLSNYEEKTI 84
           N+SFSLSG+ SKSYSKFIT+LR ALP+  KV NIPLLLP+  G++RY LM+LSNY+ K I
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQGTKHILLPYSGNYQKLQIAADKD 144
           T+AIDVTNVYIMGYLVN+TSYFFNE  A+LAS++VF+G+  + LPYSGNY++LQ AA K 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKV 121

Query: 145 RDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTTAEASRFKYIEKQIIDRIGRNEVP 204
           R+ IPLGF A D+AI++L+HYD+ AA  AFLV+IQTTAEASRFKYIE QII RI +NEVP
Sbjct: 122 REKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIKRIPKNEVP 181

Query: 205 SLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLINDKGLSVEVTNVSSLVVTNNIKLLL 264
           S AA+SLENEWS LSKQIQ+A +NNG F+TPV +I++KG  VE+ +V+S VVTNNIKLLL
Sbjct: 182 SPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLLL 241

Query: 265 NKQNVA 271
           NKQN+A
Sbjct: 242 NKQNIA 247

[Close]
BLAST of CmoCh17G005950 vs. NCBI nr
Match: gi|530776893|emb|CCD28501.1| (ribosome inactivating protein, partial [Momordica charantia])

HSP 1 Score: 328.2 bits (840), Expect = 1.4e-86
Identity = 176/269 (65.43%), Postives = 213/269 (79.18%), Query Frame = 1 

Query: 3   RLSVLSLLILTIFHEGHTAVGDNLSFSLSGSSSKSYSKFITSLRNALPNAGKVYNIPLLL 62
           R SVLS LIL IF  G    GD +SF LSG+  +SY  FI  LRNALP   KVYNIPLLL
Sbjct: 1   RFSVLSFLILAIFLGGSIVKGD-VSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLL 60

Query: 63  PNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASEFVFQG 122
           P++ G+ RY LM L NY+ KTITVA+DVTNVYIMGYL +TTSYFFNEPAA+LAS++VF+ 
Sbjct: 61  PSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRD 120

Query: 123 TKH-ILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVLIQTT 182
            +  I LPYSGNY++LQIAA K R+ IP+G LALD+AIS L HYD+ AA  A LVLIQTT
Sbjct: 121 ARRKITLPYSGNYERLQIAAGKPREKIPIGLLALDSAISTLLHYDSTAAAGALLVLIQTT 180

Query: 183 AEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVKLIND 242
           AEA+RFKYIE+QI +R  R+EVPSLA ISLEN WS LSKQIQ+A  NNG F+TP+ L+++
Sbjct: 181 AEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDN 240

Query: 243 KGLSVEVTNVSSLVVTNNIKLLLNKQNVA 271
           KG  V++TNV+S VVT+NI+LLLN +N+A
Sbjct: 241 KGNRVQITNVTSKVVTSNIQLLLNTRNIA 268

The following BLAST results are available for this feature:
BLAST of CmoCh17G005950 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RIP1_CUCFI1.5e-10774.29Putative ribosome-inactivating protein OS=Cucumis ficifolius PE=2 SV=1[more]
RIPA_LUFAE1.5e-9168.94Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca PE=1 SV=1[more]
RIP1_MOMCH1.2e-8864.94Ribosome-inactivating protein momordin I OS=Momordica charantia PE=1 SV=2[more]
RIP_LUFAC5.4e-8670.12Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula PE=1 SV=2[more]
RIPB_LUFAE9.2e-8667.07Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca PE=1 SV=1[more]
BLAST of CmoCh17G005950 vs. TrEMBL
Match NameE-valueIdentityDescription
Q00980_LUFAE4.6e-9267.78rRNA N-glycosidase OS=Luffa aegyptiaca PE=2 SV=1[more]
B0EVM6_LUFAC4.6e-9267.78rRNA N-glycosidase OS=Luffa acutangula GN=RIPI PE=2 SV=1[more]
S6CNX5_MOMCH9.9e-8765.43Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
S6CNH1_MOMCH2.2e-8665.43Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
S6CNX9_MOMCH4.9e-8665.06Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
BLAST of CmoCh17G005950 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of CmoCh17G005950 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|14195121|sp|Q9FRX4.1|RIP1_CUCFI2.3e-10574.29RecName: Full=Putative ribosome-inactivating protein; AltName: Full=rRNA N-glyco... [more]
gi|19150|emb|CAA44230.1|6.6e-9267.78b-luffin [Luffa aegyptiaca][more]
gi|132590|sp|Q00465.1|RIPA_LUFAE2.4e-8968.94RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g... [more]
gi|34810785|pdb|1NIO|A2.6e-8869.92Chain A, Crystal Structure Of Beta-Luffin, A Ribosome Inactivating Protein At 2.... [more]
gi|530776893|emb|CCD28501.1|1.4e-8665.43ribosome inactivating protein, partial [Momordica charantia][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001574Ribosome_inactivat_prot
IPR016138Ribosome_inactivat_prot_sub1
IPR016139Ribosome_inactivat_prot_sub2
IPR017988Ribosome_inactivat_prot_CS
IPR017989Ribosome_inactivat_1/2
Vocabulary: Biological Process
TermDefinition
GO:0017148negative regulation of translation
Vocabulary: Molecular Function
TermDefinition
GO:0030598rRNA N-glycosylase activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051607 defense response to virus
biological_process GO:0017148 negative regulation of translation
cellular_component GO:0005575 cellular_component
molecular_function GO:0030598 rRNA N-glycosylase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh17G005950.1CmoCh17G005950.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 34..223
score: 5.5
IPR001574Ribosome-inactivating proteinunknownSSF56371Ribosome inactivating proteins (RIP)coord: 26..268
score: 3.14
IPR016138Ribosome-inactivating protein, subdomain 1GENE3DG3DSA:3.40.420.10coord: 26..184
score: 8.4
IPR016139Ribosome-inactivating protein, subdomain 2GENE3DG3DSA:4.10.470.10coord: 186..268
score: 3.5
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 178..194
scor
IPR017989Ribosome-inactivating protein subgroupPRINTSPR00396SHIGARICINcoord: 83..98
score: 1.1E-33coord: 36..49
score: 1.1E-33coord: 174..194
score: 1.1E-33coord: 206..223
score: 1.1E-33coord: 145..159
score: 1.1

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh17G005950Cucurbita pepo (Zucchini)cmocpeB335
CmoCh17G005950Cucurbita pepo (Zucchini)cmocpeB356
CmoCh17G005950Bottle gourd (USVL1VR-Ls)cmolsiB324
CmoCh17G005950Cucumber (Gy14) v2cgybcmoB740
CmoCh17G005950Melon (DHL92) v3.6.1cmomedB355
CmoCh17G005950Silver-seed gourdcarcmoB0118
CmoCh17G005950Silver-seed gourdcarcmoB1037
CmoCh17G005950Cucumber (Chinese Long) v3cmocucB0439
CmoCh17G005950Watermelon (97103) v2cmowmbB356
CmoCh17G005950Wax gourdcmowgoB0460
CmoCh17G005950Wax gourdcmowgoB0467
CmoCh17G005950Cucurbita moschata (Rifu)cmocmoB327
CmoCh17G005950Cucumber (Gy14) v1cgycmoB1002
CmoCh17G005950Cucurbita maxima (Rimu)cmacmoB353
CmoCh17G005950Cucurbita maxima (Rimu)cmacmoB903
CmoCh17G005950Wild cucumber (PI 183967)cmocpiB367
CmoCh17G005950Cucumber (Chinese Long) v2cmocuB363
CmoCh17G005950Melon (DHL92) v3.5.1cmomeB310
CmoCh17G005950Watermelon (Charleston Gray)cmowcgB320
CmoCh17G005950Watermelon (97103) v1cmowmB350