CmoCh17G005930 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh17G005930
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
Description(Ribosome inactivating protein) (3.2.2.22)
LocationCmo_Chr17 : 6299793 .. 6300620 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATAGCGTGTCGGTGTTTTGTTTGGTAATTCTGGCCATCTTCCATGGAAGTTCAAGTTGTGTTGCGGGTGACGTGAGCTTCAACCTGTTGGGTTCGAATTCCAAATCCTATAGCAACTTCATCGAAGCCCTGAGGAGTGCTCTTCCATCCAAGGACAAAGTATACAATATATCACTATTGCTTGGCTCGGCAACAGGCGCAAGTCGCTACACACAAGTGAAACTCTCCAATTATGACGGGAAAGCCATCACGGTGGCAATAGATGTAACAAACGTTTACATCATGGGGTATCTGGTGAACTCAACTTCCTACTTCTTCAACGAATCTGATGCCCAATTGGCTTCCAAATATGTATTCAAAGACAGCACCCGTATTACACTTCCATACTCCGGCAATTATGAAAAGCTTCAAAATGCGGCAGGCAAAAAAAGGGAAAAAATTCCCCTTGGATTCCCAGCTTTGGACAATGCAATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTTATCGTGGTGATTCAGTGCACGGCTGAGGCTTCAAGATACAGATACATCGAGGCCCAAATGATTAAGAGAATTTCCAAAGACGATGTCCCAAGTCTGGCGATTATCAGTTTAGAAAACAGCTGGTCTGCTCTCTCCAAACAAATTCAATTGGCTCAAACAAATAAAGGAGTTTTTAAAGCTCCGGTTTTGATTACAGACGATCAAGGCCAACGAGTTGAAATAACCAATGTCAGTTCCAAGGTTGTAGCCAAAAACATCCAGTTGCTACTAAACTCAAAAAATATTGCAGATTTGAGCTACAATTAG

mRNA sequence

ATGAATAGCGTGTCGGTGTTTTGTTTGGTAATTCTGGCCATCTTCCATGGAAGTTCAAGTTGTGTTGCGGGTGACGTGAGCTTCAACCTGTTGGGTTCGAATTCCAAATCCTATAGCAACTTCATCGAAGCCCTGAGGAGTGCTCTTCCATCCAAGGACAAAGTATACAATATATCACTATTGCTTGGCTCGGCAACAGGCGCAAGTCGCTACACACAAGTGAAACTCTCCAATTATGACGGGAAAGCCATCACGGTGGCAATAGATGTAACAAACGTTTACATCATGGGGTATCTGGTGAACTCAACTTCCTACTTCTTCAACGAATCTGATGCCCAATTGGCTTCCAAATATGTATTCAAAGACAGCACCCGTATTACACTTCCATACTCCGGCAATTATGAAAAGCTTCAAAATGCGGCAGGCAAAAAAAGGGAAAAAATTCCCCTTGGATTCCCAGCTTTGGACAATGCAATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTTATCGTGGTGATTCAGTGCACGGCTGAGGCTTCAAGATACAGATACATCGAGGCCCAAATGATTAAGAGAATTTCCAAAGACGATGTCCCAAGTCTGGCGATTATCAGTTTAGAAAACAGCTGGTCTGCTCTCTCCAAACAAATTCAATTGGCTCAAACAAATAAAGGAGTTTTTAAAGCTCCGGTTTTGATTACAGACGATCAAGGCCAACGAGTTGAAATAACCAATGTCAGTTCCAAGGTTGTAGCCAAAAACATCCAGTTGCTACTAAACTCAAAAAATATTGCAGATTTGAGCTACAATTAG

Coding sequence (CDS)

ATGAATAGCGTGTCGGTGTTTTGTTTGGTAATTCTGGCCATCTTCCATGGAAGTTCAAGTTGTGTTGCGGGTGACGTGAGCTTCAACCTGTTGGGTTCGAATTCCAAATCCTATAGCAACTTCATCGAAGCCCTGAGGAGTGCTCTTCCATCCAAGGACAAAGTATACAATATATCACTATTGCTTGGCTCGGCAACAGGCGCAAGTCGCTACACACAAGTGAAACTCTCCAATTATGACGGGAAAGCCATCACGGTGGCAATAGATGTAACAAACGTTTACATCATGGGGTATCTGGTGAACTCAACTTCCTACTTCTTCAACGAATCTGATGCCCAATTGGCTTCCAAATATGTATTCAAAGACAGCACCCGTATTACACTTCCATACTCCGGCAATTATGAAAAGCTTCAAAATGCGGCAGGCAAAAAAAGGGAAAAAATTCCCCTTGGATTCCCAGCTTTGGACAATGCAATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTTATCGTGGTGATTCAGTGCACGGCTGAGGCTTCAAGATACAGATACATCGAGGCCCAAATGATTAAGAGAATTTCCAAAGACGATGTCCCAAGTCTGGCGATTATCAGTTTAGAAAACAGCTGGTCTGCTCTCTCCAAACAAATTCAATTGGCTCAAACAAATAAAGGAGTTTTTAAAGCTCCGGTTTTGATTACAGACGATCAAGGCCAACGAGTTGAAATAACCAATGTCAGTTCCAAGGTTGTAGCCAAAAACATCCAGTTGCTACTAAACTCAAAAAATATTGCAGATTTGAGCTACAATTAG
Homology
[Close]
BLAST of CmoCh17G005930 vs. Swiss-Prot
Match: RIPA_LUFAE (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-104
Identity = 206/265 (77.74%), Postives = 231/265 (87.17%), Query Frame = 1 

Query: 7   FCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSAT 66
           F ++ILAIF  +S+ V  DV F+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+
Sbjct: 4   FTVLILAIFVAAST-VEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSAS 63

Query: 67  GASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRI 126
           GASRYT + LSNYDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +
Sbjct: 64  GASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIV 123

Query: 127 TLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASR 186
           TLPYSGNYEKLQ AAGK REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAEASR
Sbjct: 124 TLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASR 183

Query: 187 YRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRV 246
           ++YIE Q+I+RISK+ VPSLA ISLEN WSALSKQIQLAQTN G FK PV+ITDD+GQRV
Sbjct: 184 FKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRV 243

Query: 247 EITNVSSKVVAKNIQLLLNSK-NIA 271
           EITNV+SKVV KNIQLLLN K N+A
Sbjct: 244 EITNVTSKVVTKNIQLLLNYKQNVA 267

[Close]
BLAST of CmoCh17G005930 vs. Swiss-Prot
Match: RIP_LUFAC (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula PE=1 SV=2)

HSP 1 Score: 365.2 bits (936), Expect = 6.5e-100
Identity = 194/241 (80.50%), Postives = 211/241 (87.55%), Query Frame = 1 

Query: 25  DVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAI 84
           DVSF+L GS+S SYS FI ALR ALPS   VYNI+LLL SA+GASRYT +KLSNYDGKAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 85  TVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKK 144
           TVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF  ST +TLPYSGNYEKLQ AAGK 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 145 REKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKRISKDDVP 204
           REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAE+SR++YIE Q+I RISK+ VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 205 SLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVAKNIQLLL 264
           SLA ISLEN WSALSKQIQLAQTN G FK PV+I D  GQRVEI NV SKVV KNIQLLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 265 N 266
           N
Sbjct: 241 N 241

[Close]
BLAST of CmoCh17G005930 vs. Swiss-Prot
Match: RIPB_LUFAE (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 5.0e-92
Identity = 180/249 (72.29%), Postives = 217/249 (87.15%), Query Frame = 1 

Query: 25  DVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAI 84
           +VSF+L G++SKSYS FI ALR ALPSK+KV NI LLL SA+GASRY  ++LSNYD KAI
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKK 144
           T+AIDVTNVYIMGYLVNSTSYF NESDA+LAS+YVFK ST +T+PYSGNYE+LQNAAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 145 REKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKRISKDDVP 204
           REKIPLGF ALD+A+T++FHYDSTAAAAAF+V++Q TAEASR++YIE Q+I+RI K++VP
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 205 SLAIISLEN-SWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVAK--NIQ 264
           S A +SLEN +WS LSKQIQLAQTN G F+ PV+I D++GQRVEITN++SKV  K  N +
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

Query: 265 LLLNSKNIA 271
           LLLN +NIA
Sbjct: 242 LLLNKQNIA 250

[Close]
BLAST of CmoCh17G005930 vs. Swiss-Prot
Match: RIP1_MOMCH (Ribosome-inactivating protein momordin I OS=Momordica charantia PE=1 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 3.2e-91
Identity = 178/272 (65.44%), Postives = 221/272 (81.25%), Query Frame = 1 

Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           M+  SV   +ILAIF G S  V GDVSF L G++ +SY  FI+ LR+ALP ++KVYNI L
Sbjct: 1   MSRFSVLSFLILAIFLGGS-IVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL S +GA RY  + L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LAS+YVF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KDSTR-ITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQ 180
           +D+ R ITLPYSGNYE+LQ AAGK REKIP+G PALD+AI+TL HYDSTAAA A +V+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 CTAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLIT 240
            TAEA+R++YIE Q+ +R  +D+VPSLA ISLENSWS LSKQIQLAQ N G+F+ P+++ 
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DDQGQRVEITNVSSKVVAKNIQLLLNSKNIAD 272
           D++G RV+ITNV+SKVV  NIQLLLN++NIA+
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIAE 271

[Close]
BLAST of CmoCh17G005930 vs. Swiss-Prot
Match: RIP1_CUCFI (Putative ribosome-inactivating protein OS=Cucumis ficifolius PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.5e-85
Identity = 172/273 (63.00%), Postives = 214/273 (78.39%), Query Frame = 1 

Query: 1   MNSVSVF-CLVILAIFHGSSSCVAGDVS--FNLLGSNSKSYSNFIEALRSALPSKDKVYN 60
           MN  SV  CLVIL+IFHG  +   GDV+  F+LLGSN KSYS FI ++R+ALP+   +YN
Sbjct: 1   MNRFSVLMCLVILSIFHGVPT-AEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYN 60

Query: 61  ISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASK 120
           I LL+ S +G+ RY  ++LSNY+G  IT+A+DVTNVYIMGYLVN TSYFFNE+DAQLASK
Sbjct: 61  IPLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASK 120

Query: 121 YVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVV 180
           +VF+ +  ITLPYSGNY+KLQ+ A K+R+ IPLGF ALD+AI+TL++YDS +A  AF+V+
Sbjct: 121 FVFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVL 180

Query: 181 IQCTAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVL 240
           IQ TAEA+RY+YIE Q+I RIS   VP LA ISLEN WS LSKQIQ+A++N G F+ PV 
Sbjct: 181 IQTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVK 240

Query: 241 ITDDQGQRVEITNVSSKVVAKNIQLLLNSKNIA 271
           I +D+G   E+TNVSS VV KNI LLLN  NIA
Sbjct: 241 IINDKGILTEVTNVSSLVVTKNIMLLLNKLNIA 272

[Close]
BLAST of CmoCh17G005930 vs. TrEMBL
Match: B0EVM6_LUFAC (rRNA N-glycosidase OS=Luffa acutangula GN=RIPI PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 8.3e-94
Identity = 189/270 (70.00%), Postives = 225/270 (83.33%), Query Frame = 1 

Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MN  +   L+I+ I     +    +VSF+L G++SKSYS FI ALR ALPSK+KV NI L
Sbjct: 1   MNRFTFLSLLIILI--AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL SA+GASRY  ++LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           K ST +TLPYSGNYE+LQNAAGK REKIPLGF A D+AIT+LFHYDSTAAA AF+V+IQ 
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASR++YIE Q+I+RI K++VPS A +SLEN WSALSKQIQLAQTN G F+ PV+I D
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 271
           ++GQRVEI +V+SKVV  NI+LLLN +NIA
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]
BLAST of CmoCh17G005930 vs. TrEMBL
Match: Q00980_LUFAE (rRNA N-glycosidase OS=Luffa aegyptiaca PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 8.3e-94
Identity = 189/270 (70.00%), Postives = 225/270 (83.33%), Query Frame = 1 

Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MN  +   L+I+ I     +    +VSF+L G++SKSYS FI ALR ALPSK+KV NI L
Sbjct: 1   MNRFTFLSLLIILI--AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL SA+GASRY  ++LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           K ST +TLPYSGNYE+LQNAAGK REKIPLGF A D+AIT+LFHYDSTAAA AF+V+IQ 
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASR++YIE Q+I+RI K++VPS A +SLEN WSALSKQIQLAQTN G F+ PV+I D
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 271
           ++GQRVEI +V+SKVV  NI+LLLN +NIA
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]
BLAST of CmoCh17G005930 vs. TrEMBL
Match: S6CNX9_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.0e-88
Identity = 177/268 (66.04%), Postives = 219/268 (81.72%), Query Frame = 1 

Query: 5   SVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGS 64
           SV   +ILAIF G S  V GDVSF L G++ +SY  FI+ LR+ALP ++KVYNI LLL S
Sbjct: 3   SVLSFLILAIFLGGS-IVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLLPS 62

Query: 65  ATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDST 124
            +GA RY  + L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LAS+YVF+D+ 
Sbjct: 63  VSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRDAR 122

Query: 125 R-ITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAE 184
           R ITLPYSGNYE+LQ AAGK REKIP+G PALD+AI+TL HYDSTAAA A +V+IQ TAE
Sbjct: 123 RKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQTTAE 182

Query: 185 ASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQG 244
           A+R++YIE Q+ +R  +D+VPSLA ISLENSWS LSKQIQLAQ N G+F+ P+++ D++G
Sbjct: 183 AARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDNKG 242

Query: 245 QRVEITNVSSKVVAKNIQLLLNSKNIAD 272
            RV+ITNV+SKVV  NIQLLLN++NIA+
Sbjct: 243 SRVQITNVTSKVVTSNIQLLLNTRNIAE 269

[Close]
BLAST of CmoCh17G005930 vs. TrEMBL
Match: S6CNX6_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.0e-88
Identity = 177/268 (66.04%), Postives = 219/268 (81.72%), Query Frame = 1 

Query: 5   SVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGS 64
           SV   +ILAIF G S  V GDVSF L G++ +SY  FI+ LR+ALP ++KVYNI LLL S
Sbjct: 3   SVLSFLILAIFLGGS-IVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLLPS 62

Query: 65  ATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDST 124
            +GA RY  + L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LAS+YVF+D+ 
Sbjct: 63  VSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRDAR 122

Query: 125 R-ITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAE 184
           R ITLPYSGNYE+LQ AAGK REKIP+G PALD+AI+TL HYDSTAAA A +V+IQ TAE
Sbjct: 123 RKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQTTAE 182

Query: 185 ASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQG 244
           A+R++YIE Q+ +R  +D+VPSLA ISLENSWS LSKQIQLAQ N G+F+ P+++ D++G
Sbjct: 183 AARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDNKG 242

Query: 245 QRVEITNVSSKVVAKNIQLLLNSKNIAD 272
            RV+ITNV+SKVV  NIQLLLN++NIA+
Sbjct: 243 NRVQITNVTSKVVTSNIQLLLNTRNIAE 269

[Close]
BLAST of CmoCh17G005930 vs. TrEMBL
Match: S6CNX3_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.0e-88
Identity = 177/268 (66.04%), Postives = 219/268 (81.72%), Query Frame = 1 

Query: 5   SVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGS 64
           SV   +ILAIF G S  V GDVSF L G++ +SY  FI+ LR+ALP ++KVYNI LLL S
Sbjct: 3   SVLSFLILAIFLGGS-IVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLLPS 62

Query: 65  ATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDST 124
            +GA RY  + L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LAS+YVF+D+ 
Sbjct: 63  VSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRDAR 122

Query: 125 R-ITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAE 184
           R ITLPYSGNYE+LQ AAGK REKIP+G PALD+AI+TL HYDSTAAA A +V+IQ TAE
Sbjct: 123 RKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQTTAE 182

Query: 185 ASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQG 244
           A+R++YIE Q+ +R  +D+VPSLA ISLENSWS LSKQIQLAQ N G+F+ P+++ D++G
Sbjct: 183 AARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDNKG 242

Query: 245 QRVEITNVSSKVVAKNIQLLLNSKNIAD 272
            RV+ITNV+SKVV  NIQLLLN++NIA+
Sbjct: 243 NRVQITNVTSKVVTSNIQLLLNTRNIAE 269

[Close]
BLAST of CmoCh17G005930 vs. NCBI nr
Match: gi|132590|sp|Q00465.1|RIPA_LUFAE (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 380.6 bits (976), Expect = 2.4e-102
Identity = 206/265 (77.74%), Postives = 231/265 (87.17%), Query Frame = 1 

Query: 7   FCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSAT 66
           F ++ILAIF  +S+ V  DV F+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+
Sbjct: 4   FTVLILAIFVAAST-VEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSAS 63

Query: 67  GASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRI 126
           GASRYT + LSNYDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +
Sbjct: 64  GASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIV 123

Query: 127 TLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASR 186
           TLPYSGNYEKLQ AAGK REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAEASR
Sbjct: 124 TLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASR 183

Query: 187 YRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRV 246
           ++YIE Q+I+RISK+ VPSLA ISLEN WSALSKQIQLAQTN G FK PV+ITDD+GQRV
Sbjct: 184 FKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRV 243

Query: 247 EITNVSSKVVAKNIQLLLNSK-NIA 271
           EITNV+SKVV KNIQLLLN K N+A
Sbjct: 244 EITNVTSKVVTKNIQLLLNYKQNVA 267

[Close]
BLAST of CmoCh17G005930 vs. NCBI nr
Match: gi|284018116|sp|P84530.2|RIP_LUFAC (RecName: Full=Ribosome-inactivating protein luffaculin 1; AltName: Full=rRNA N-glycosidase [Luffa acutangula])

HSP 1 Score: 365.2 bits (936), Expect = 1.0e-97
Identity = 194/241 (80.50%), Postives = 211/241 (87.55%), Query Frame = 1 

Query: 25  DVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAI 84
           DVSF+L GS+S SYS FI ALR ALPS   VYNI+LLL SA+GASRYT +KLSNYDGKAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 85  TVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKK 144
           TVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF  ST +TLPYSGNYEKLQ AAGK 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 145 REKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKRISKDDVP 204
           REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAE+SR++YIE Q+I RISK+ VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 205 SLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVAKNIQLLL 264
           SLA ISLEN WSALSKQIQLAQTN G FK PV+I D  GQRVEI NV SKVV KNIQLLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 265 N 266
           N
Sbjct: 241 N 241

[Close]
BLAST of CmoCh17G005930 vs. NCBI nr
Match: gi|34810785|pdb|1NIO|A (Chain A, Crystal Structure Of Beta-Luffin, A Ribosome Inactivating Protein At 2.0a Resolution)

HSP 1 Score: 354.8 bits (909), Expect = 1.4e-94
Identity = 185/246 (75.20%), Postives = 216/246 (87.80%), Query Frame = 1 

Query: 25  DVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAI 84
           +VSF+L G++SKSYS FI ALR ALPSK+KV NI LLL SA+GASRY  ++LSNYD KAI
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKK 144
           T+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +TLPYSGNYE+LQNAAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKV 121

Query: 145 REKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKRISKDDVP 204
           REKIPLGF A D+AIT+LFHYDSTAAA AF+V+IQ TAEASR++YIE Q+IKRI K++VP
Sbjct: 122 REKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFKYIEGQIIKRIPKNEVP 181

Query: 205 SLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVAKNIQLLL 264
           S A +SLEN WSALSKQIQLAQTN G F+ PV+I D++GQRVEI +V+SKVV  NI+LLL
Sbjct: 182 SPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEIKDVNSKVVTNNIKLLL 241

Query: 265 NSKNIA 271
           N +NIA
Sbjct: 242 NKQNIA 247

[Close]
BLAST of CmoCh17G005930 vs. NCBI nr
Match: gi|19150|emb|CAA44230.1| (b-luffin [Luffa aegyptiaca])

HSP 1 Score: 351.7 bits (901), Expect = 1.2e-93
Identity = 189/270 (70.00%), Postives = 225/270 (83.33%), Query Frame = 1 

Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MN  +   L+I+ I     +    +VSF+L G++SKSYS FI ALR ALPSK+KV NI L
Sbjct: 1   MNRFTFLSLLIILI--AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL SA+GASRY  ++LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           K ST +TLPYSGNYE+LQNAAGK REKIPLGF A D+AIT+LFHYDSTAAA AF+V+IQ 
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASR++YIE Q+I+RI K++VPS A +SLEN WSALSKQIQLAQTN G F+ PV+I D
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 271
           ++GQRVEI +V+SKVV  NI+LLLN +NIA
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]
BLAST of CmoCh17G005930 vs. NCBI nr
Match: gi|132591|sp|P22851.1|RIPB_LUFAE (RecName: Full=Ribosome-inactivating protein luffin-B; AltName: Full=rRNA N-glycosidase)

HSP 1 Score: 339.0 bits (868), Expect = 7.9e-90
Identity = 180/249 (72.29%), Postives = 217/249 (87.15%), Query Frame = 1 

Query: 25  DVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAI 84
           +VSF+L G++SKSYS FI ALR ALPSK+KV NI LLL SA+GASRY  ++LSNYD KAI
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKK 144
           T+AIDVTNVYIMGYLVNSTSYF NESDA+LAS+YVFK ST +T+PYSGNYE+LQNAAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 145 REKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKRISKDDVP 204
           REKIPLGF ALD+A+T++FHYDSTAAAAAF+V++Q TAEASR++YIE Q+I+RI K++VP
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 205 SLAIISLEN-SWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVAK--NIQ 264
           S A +SLEN +WS LSKQIQLAQTN G F+ PV+I D++GQRVEITN++SKV  K  N +
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

Query: 265 LLLNSKNIA 271
           LLLN +NIA
Sbjct: 242 LLLNKQNIA 250

The following BLAST results are available for this feature:
BLAST of CmoCh17G005930 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RIPA_LUFAE1.5e-10477.74Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca PE=1 SV=1[more]
RIP_LUFAC6.5e-10080.50Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula PE=1 SV=2[more]
RIPB_LUFAE5.0e-9272.29Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca PE=1 SV=1[more]
RIP1_MOMCH3.2e-9165.44Ribosome-inactivating protein momordin I OS=Momordica charantia PE=1 SV=2[more]
RIP1_CUCFI1.5e-8563.00Putative ribosome-inactivating protein OS=Cucumis ficifolius PE=2 SV=1[more]
BLAST of CmoCh17G005930 vs. TrEMBL
Match NameE-valueIdentityDescription
B0EVM6_LUFAC8.3e-9470.00rRNA N-glycosidase OS=Luffa acutangula GN=RIPI PE=2 SV=1[more]
Q00980_LUFAE8.3e-9470.00rRNA N-glycosidase OS=Luffa aegyptiaca PE=2 SV=1[more]
S6CNX9_MOMCH1.0e-8866.04Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
S6CNX6_MOMCH1.0e-8866.04Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
S6CNX3_MOMCH1.0e-8866.04Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
BLAST of CmoCh17G005930 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of CmoCh17G005930 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|132590|sp|Q00465.1|RIPA_LUFAE2.4e-10277.74RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g... [more]
gi|284018116|sp|P84530.2|RIP_LUFAC1.0e-9780.50RecName: Full=Ribosome-inactivating protein luffaculin 1; AltName: Full=rRNA N-g... [more]
gi|34810785|pdb|1NIO|A1.4e-9475.20Chain A, Crystal Structure Of Beta-Luffin, A Ribosome Inactivating Protein At 2.... [more]
gi|19150|emb|CAA44230.1|1.2e-9370.00b-luffin [Luffa aegyptiaca][more]
gi|132591|sp|P22851.1|RIPB_LUFAE7.9e-9072.29RecName: Full=Ribosome-inactivating protein luffin-B; AltName: Full=rRNA N-glyco... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001574Ribosome_inactivat_prot
IPR016138Ribosome_inactivat_prot_sub1
IPR016139Ribosome_inactivat_prot_sub2
IPR017989Ribosome_inactivat_1/2
Vocabulary: Biological Process
TermDefinition
GO:0017148negative regulation of translation
Vocabulary: Molecular Function
TermDefinition
GO:0030598rRNA N-glycosylase activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051607 defense response to virus
biological_process GO:0017148 negative regulation of translation
cellular_component GO:0005575 cellular_component
molecular_function GO:0030598 rRNA N-glycosylase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh17G005930.1CmoCh17G005930.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 35..223
score: 2.1
IPR001574Ribosome-inactivating proteinunknownSSF56371Ribosome inactivating proteins (RIP)coord: 25..267
score: 1.02
IPR016138Ribosome-inactivating protein, subdomain 1GENE3DG3DSA:3.40.420.10coord: 25..184
score: 2.3
IPR016139Ribosome-inactivating protein, subdomain 2GENE3DG3DSA:4.10.470.10coord: 186..268
score: 1.6
IPR017989Ribosome-inactivating protein subgroupPRINTSPR00396SHIGARICINcoord: 145..159
score: 1.4E-33coord: 174..194
score: 1.4E-33coord: 36..49
score: 1.4E-33coord: 83..98
score: 1.4E-33coord: 206..223
score: 1.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmoCh17G005930CmaCh17G006110Cucurbita maxima (Rimu)cmacmoB353
CmoCh17G005930Carg18910Silver-seed gourdcarcmoB1037
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh17G005930Cucurbita moschata (Rifu)cmocmoB327
CmoCh17G005930Cucumber (Gy14) v1cgycmoB1002
CmoCh17G005930Cucurbita maxima (Rimu)cmacmoB903
CmoCh17G005930Wild cucumber (PI 183967)cmocpiB367
CmoCh17G005930Cucumber (Chinese Long) v2cmocuB363
CmoCh17G005930Melon (DHL92) v3.5.1cmomeB310
CmoCh17G005930Watermelon (Charleston Gray)cmowcgB320
CmoCh17G005930Watermelon (97103) v1cmowmB350
CmoCh17G005930Cucurbita pepo (Zucchini)cmocpeB335
CmoCh17G005930Cucurbita pepo (Zucchini)cmocpeB356
CmoCh17G005930Bottle gourd (USVL1VR-Ls)cmolsiB324
CmoCh17G005930Cucumber (Gy14) v2cgybcmoB740
CmoCh17G005930Melon (DHL92) v3.6.1cmomedB355
CmoCh17G005930Silver-seed gourdcarcmoB0118
CmoCh17G005930Cucumber (Chinese Long) v3cmocucB0439
CmoCh17G005930Watermelon (97103) v2cmowmbB356
CmoCh17G005930Wax gourdcmowgoB0460
CmoCh17G005930Wax gourdcmowgoB0467