CmoCh17G003910 (gene) Cucurbita moschata (Rifu)

NameCmoCh17G003910
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptiontRNA pseudouridine synthase (5.4.99.-)
LocationCmo_Chr17 : 2448557 .. 2452285 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTACTGGGCGGTGGGTTCTGCGGTTCTTCCCTCCGTTCCCCGTCTTCTTTCACCAAGCATCCCATCTCGAAATGGCGATTCATCTCCCACCGGTCGTGGATTTCCGGTAACGGTTCACGAATCTCTCATCTCGTCTTCTCATTGTTGTGGTTTAATTTTGACGATTCTGATGAGGTATTTGTCTTGTCTTGATTCTTAAAAAGATTTCGGAGGATTCTTCTTGTGTTACAGAGCCCGATTTCCCCAGCTTCAAATGGCGGTTGGTCATTGCTTATGACGGCACTCGATATGCAGGTTGAGATTTCTGTTTCTTCTCTCTAGTTGTCTATTCAGGCGAATGAATTGAACGATTTTATGTCTGCATTCCAGCTTTATCTTTTTAGGTCGATGTTAATTCTAAATGGATTAAGATATTTGATCAACAAGAATCATTGAAGCTAGACATGATCGATTCGAATCTCAAACAAAGTGTTCTTGGCTTGAGATATTCGATCTACAAGGTAATCCGAATCTTACTCCCCTTGTAGTGATTCCTTATCGTATCATTAATGATAAGATAAGGAATCAATACAAGGGGAATAAGATTCGGATGATAAGATAAGGAATCAATACAAGGGGAATAAGATTCGGATTACCTTGTTGATCGAATATCTCAAGACAAGAACATTGTTTGAGATTCGAATCACTCCACAAGCAAGATCGATCATGTCTAGCTTGAATGATTCTTGTTGATCAAATATCTCAACGCAAGAACACTTGATTGAGATTCGAATCACTCCACAAGCAAGATTGATCATGTCGAGCTTGAATGATTCTACATGCAACCTAAACTACATAGAGTTGCAAAGAAACTTAGCCATTGGCTAAAGAAAAGCACAAATGCTTCTTTTAATATATTTTCCAAGTCTTCCTTACGAATACAACATACATGGCTTTATATAGCCTCAAAATGAAAGCATTGTATGGCTTTATATAGCCTCAAAATGAAAGCATTCCAAAAGTTGTAACATTCATACTTAATGACCATAATTAGCCATTATGTAAATGTAACCTATAAAGTAAATAAAATGTCTTAAAATACAATAACTCTAAATTACTCGTAACCCACCCAAAATTTATAACCATCAAACTTCATTCCTCTTCAATGTGACATGAATTGCAACCTCTTTTGATAATTTTGACATCATTTTCTTCACATCTTCATTGAAGTATATTGTATGAATGATGTCTCTTGGTTCATATCAGTTTCTGTTGGATGTCTTTTTCATCATCGTAATATATTCTAAATGCTAATACCAAAATGCTGTTGGAATATTCAGTATTTTTTTTGTGTTCTTACAGAATGGATGAAGAAATTATTTTTCTTTCTCCTTGGAAATTGTAGAGAATCTCTCAAAGAATCAGTGTTCTTTCTGGAAAATGAACTCATTGTATCCTAGCAGTTACAAAAAACTGTAAAAGGATACGTTGAGATAACGTATATTTAATTAATCACTTAATCAAATAACAAATATTTAATTACAAACAATTTGTAATAACTTTCCATGGTTGTGTTTAGTTTATATTAGCTAGCTCAAAGCTTTGACATGATTTGGGGTCTGGTGTGAAGTGTTGTGCGTTAACTCAGAGAATTTTGCGCTCATTACTTCAATTTTGAGGCTCTCTTGGCCCAAAAGTTTGATAAATAATTTTGAGTTTGTTGATGCTGAATTAGGTTGGCAATATCAGCGGTCACCACCTACTATTCAGTGCCTTGTTGAAGAAGCCTTGACCGGAGCAACAAGGCTTGAGAGGAAGGATCTCCATTTAGTTGGTGCAAGCAGAACAGATACGGGAGTTCATGCCTGGGGTCAGGTCTGATGTGCTTTTATGGAGTAGTCTTAAAACACGTCCTTGAAAAACAATGCTTGCTGTTGCTTACAACAAGTTGCAGGTTTCAGGTGGCTCACTTTATTACACCATTTAACTACGACAGCTTGGACAGAATTCATGCTGCTCTGAATGGTCTTCTTCCTGCGGATATAAGAGTTCGAGAGATGAGTCCTGCTGTGCCTGACTTTCATGCTCGGTTTTCTACATTAAGCAAGGTTTATCACTACAAGATATATAATGACGCCATCTTGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAATTATGCAGCCATGAAAGAGGCTGCAAATTATTTCGTTGGAAAGCATGATTTTTCTGCCTTTGCTAATACAGCGCATAACGATCGAGTGGTAAATCCAGTGAAGGTCATTTTTCGTTTTGACGTCATAGAAATGGTAATGTTTTAACATTATTTAATCCTTGATTTGTTTGGTTAAATTAGAAGGTTAATTCATGAACTTTGAGGTTTGTATCAAACAGGTTTTTAACTTTTAAAAGTGTTCGATAAGCTCTTGAACTTTCAATTTTGTGTCTAATATGAACTTTAAAGAGGGTTTGATATGTTCATAAACTTTCAGTTTTGTGCCTAGTATATCCTTGAATTTTTAATTTTGCATCTACTTGATGTATTTTAGATCTCATACGTCTACTAGATACAAATTAAAAGTTTAGAGTTTCATTAGACATAAAAGTCGAAATTATATTTAATAAATCAATGTTTTATAGAAATTTTGGATATGTCAATTGATTTATGTGACAATTTTAAAATTTAGGGATACTTTTGATATAAGATTGAAAGTTCAAGAATCAATTAGACATTTTCGAAACTTCATGGACTAACTAGAAACATACCTAAGTTTAGGGACTAAACTTGTAATTTACTTTTTCATTTTTTCTATACTGACTGCCTCTCACGAGTCAGGTTTGGCTAGTTATGTATCCTCTTCTTTTGGTCATCTGTGCAAATCCTTCCGTTTTCAGCTTTATACAAAACCCGAGGTTAAATGCTTAGGGTGCAACTGTTTCTTTTCTTCAGGGAGCTCTTATTCAGCTAGAAGTTGAGGGTTCTGGGTTCCTGTATAGACAAGTTCGGAATATGGTAATTATATTTGTTAAACCTATCTTTTTAGACGAAGCCCCTTGTCTGATTTGTTTACTTATGCTGGTCGCTATAACGACCTTACGGTAGAAAGTAAACTCCCTGGAAGCGTAAATTTAAGTTCGTGAACGGTTTCATGCAACTGTAACCATTTTGTCCATTACTTTTGCAGGTTGCCTTGTTGCTTCAAATTGGAAAAGAAGCAATTCCTCCTGACATTGTTCCTAAGATTTTGGCTACTCGAGATCGCAAGGAGCTTGCCAAGTATACGACGTGCATTCCTCCTCAGGGTCTCTGTCTTGTGAGAATTAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTTTTGGCAGGAGTCATAGTGTGAGTAAATGTAAGCTCCCTTTTTATTGAACTAATGACGGCTCTGTTCTTGCACTTCTGCCATGAGAATAATCTGGGTTGCAGGGTGTCCAACAGCTTAGGAGGCTGAGATATTAGATAAGCAGTGTAGTTTAAGTAAACAAAATTTTGGATTAGTTGGTCTTTATTAATTTATGAATTAGAATTCTAGAGTGTGTTAGTTTGTTATTTGTCCATTTTTTCTTAGGCTAGAAGTGGGCTTTTTTAGTGTTGTATTGGGACTTTTGGAGCTTTGCACATTATTATTGCAAAACGTGGTATTTTAGAGGGTTTCTCTCAATTATGGAGAATGGAAGGTT

mRNA sequence

ATGAGTTACTGGGCGGTGGGTTCTGCGGTTCTTCCCTCCGTTCCCCGTCTTCTTTCACCAAGCATCCCATCTCGAAATGGCGATTCATCTCCCACCGGTCGTGGATTTCCGATTTCGGAGGATTCTTCTTGTGTTACAGAGCCCGATTTCCCCAGCTTCAAATGGCGGTTGGTCATTGCTTATGACGGCACTCGATATGCAGGTTGGCAATATCAGCGGTCACCACCTACTATTCAGTGCCTTGTTGAAGAAGCCTTGACCGGAGCAACAAGGCTTGAGAGGAAGGATCTCCATTTAGTTGGTGCAAGCAGAACAGATACGGGAGTTCATGCCTGGGGTCAGGTGGCTCACTTTATTACACCATTTAACTACGACAGCTTGGACAGAATTCATGCTGCTCTGAATGGTCTTCTTCCTGCGGATATAAGAGTTCGAGAGATGAGTCCTGCTGTGCCTGACTTTCATGCTCGGTTTTCTACATTAAGCAAGGTTTATCACTACAAGATATATAATGACGCCATCTTGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAATTATGCAGCCATGAAAGAGGCTGCAAATTATTTCGTTGGAAAGCATGATTTTTCTGCCTTTGCTAATACAGCGCATAACGATCGAGTGGTAAATCCAGTGAAGGTCATTTTTCGTTTTGACGTCATAGAAATGGGAGCTCTTATTCAGCTAGAAGTTGAGGGTTCTGGGTTCCTGTATAGACAAGTTCGGAATATGGTTGCCTTGTTGCTTCAAATTGGAAAAGAAGCAATTCCTCCTGACATTGTTCCTAAGATTTTGGCTACTCGAGATCGCAAGGAGCTTGCCAAGTATACGACGTGCATTCCTCCTCAGGGTCTCTGTCTTGTGAGAATTAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTTTTGGCAGGAGTCATAGTGTGAGTAAATGTAAGCTCCCTTTTTATTGAACTAATGACGGCTCTGTTCTTGCACTTCTGCCATGAGAATAATCTGGGTTGCAGGGTGTCCAACAGCTTAGGAGGCTGAGATATTAGATAAGCAGTGTAGTTTAAGTAAACAAAATTTTGGATTAGTTGGTCTTTATTAATTTATGAATTAGAATTCTAGAGTGTGTTAGTTTGTTATTTGTCCATTTTTTCTTAGGCTAGAAGTGGGCTTTTTTAGTGTTGTATTGGGACTTTTGGAGCTTTGCACATTATTATTGCAAAACGTGGTATTTTAGAGGGTTTCTCTCAATTATGGAGAATGGAAGGTT

Coding sequence (CDS)

ATGAGTTACTGGGCGGTGGGTTCTGCGGTTCTTCCCTCCGTTCCCCGTCTTCTTTCACCAAGCATCCCATCTCGAAATGGCGATTCATCTCCCACCGGTCGTGGATTTCCGATTTCGGAGGATTCTTCTTGTGTTACAGAGCCCGATTTCCCCAGCTTCAAATGGCGGTTGGTCATTGCTTATGACGGCACTCGATATGCAGGTTGGCAATATCAGCGGTCACCACCTACTATTCAGTGCCTTGTTGAAGAAGCCTTGACCGGAGCAACAAGGCTTGAGAGGAAGGATCTCCATTTAGTTGGTGCAAGCAGAACAGATACGGGAGTTCATGCCTGGGGTCAGGTGGCTCACTTTATTACACCATTTAACTACGACAGCTTGGACAGAATTCATGCTGCTCTGAATGGTCTTCTTCCTGCGGATATAAGAGTTCGAGAGATGAGTCCTGCTGTGCCTGACTTTCATGCTCGGTTTTCTACATTAAGCAAGGTTTATCACTACAAGATATATAATGACGCCATCTTGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAATTATGCAGCCATGAAAGAGGCTGCAAATTATTTCGTTGGAAAGCATGATTTTTCTGCCTTTGCTAATACAGCGCATAACGATCGAGTGGTAAATCCAGTGAAGGTCATTTTTCGTTTTGACGTCATAGAAATGGGAGCTCTTATTCAGCTAGAAGTTGAGGGTTCTGGGTTCCTGTATAGACAAGTTCGGAATATGGTTGCCTTGTTGCTTCAAATTGGAAAAGAAGCAATTCCTCCTGACATTGTTCCTAAGATTTTGGCTACTCGAGATCGCAAGGAGCTTGCCAAGTATACGACGTGCATTCCTCCTCAGGGTCTCTGTCTTGTGAGAATTAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTTTTGGCAGGAGTCATAGTGTGAGTAAATGTAAGCTCCCTTTTTATTGA
BLAST of CmoCh17G003910 vs. Swiss-Prot
Match: TRUA1_PARUW (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) GN=truA1 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.5e-54
Identity = 104/255 (40.78%), Postives = 164/255 (64.31%), Query Frame = 1

Query: 55  WRLVIAYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQ 114
           ++L IAYDGT Y+GWQ Q +  +IQ  ++EAL     L+++ + LVG+ RTD GVHA GQ
Sbjct: 4   YKLTIAYDGTNYSGWQIQPNASSIQQKIQEAL--CILLKKEKVVLVGSGRTDAGVHAKGQ 63

Query: 115 VAHFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAI 174
           VAHF    +Y  L R+H +LNGLLP DIR++ + P  P FH+++S + K YHY ++ + +
Sbjct: 64  VAHFHFQ-DYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKV 123

Query: 175 LDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVV-NPVKVIFRFDVI 234
           +DPFQR Y++H   K++   +K+AA  F G HDF++FAN AH      NPV+ ++R D+ 
Sbjct: 124 MDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIK 183

Query: 235 EMGALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIP 294
                ++LE EG GFLY+ VRN+V  L+ +       + + +I A ++R++    +   P
Sbjct: 184 PNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQA---SLAAP 243

Query: 295 PQGLCLVRINYKEDH 309
           P+GL L+++ Y+ ++
Sbjct: 244 PEGLFLIQVFYENEN 252

BLAST of CmoCh17G003910 vs. Swiss-Prot
Match: TRUA_CALS4 (tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) GN=truA PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.0e-46
Identity = 110/248 (44.35%), Postives = 147/248 (59.27%), Query Frame = 1

Query: 57  LVIAYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVA 116
           L+I YDGT Y GWQYQ++  T+Q ++E+A+   T    + ++L+G+SRTDTGVHA  QVA
Sbjct: 2   LIIEYDGTNYHGWQYQKNAITVQEVLEKAIEKVTG---EKVNLIGSSRTDTGVHALYQVA 61

Query: 117 HFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILD 176
           +F T       +++  ALN +LP D+ V +       FHAR+S  SK Y Y I N     
Sbjct: 62  NFKTKTKIPP-EKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPM 121

Query: 177 PFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMG 236
           P  R Y +H  Y L+  AMK+AA +F G HDFSAF   A  ++ VNPVK IF   V + G
Sbjct: 122 PTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAF--KASGNKKVNPVKTIFDLTVEKEG 181

Query: 237 ALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQG 296
            LI++EVE  GFLY  VR +V  L+ +G   I    +P I+ ++DR    K     PPQG
Sbjct: 182 DLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDR---TKAGITAPPQG 240

Query: 297 LCLVRINY 305
           L LVRI Y
Sbjct: 242 LYLVRIVY 240

BLAST of CmoCh17G003910 vs. Swiss-Prot
Match: TRUA_DESAP (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) GN=truA PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.5e-46
Identity = 105/251 (41.83%), Postives = 141/251 (56.18%), Query Frame = 1

Query: 56  RLVIAYDGTRYAGWQYQRSP--PTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWG 115
           RL +AYDGT Y G+Q Q     PTIQ  +E  L     L    L + GASRTD GVHA G
Sbjct: 5   RLTLAYDGTAYHGFQKQSGSGLPTIQETLERCLA---ELSGAALKVTGASRTDAGVHARG 64

Query: 116 QVAHFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDA 175
           QV +F+T       +RI AALNG+LP DI   +      DFHAR+S ++K Y Y +YN  
Sbjct: 65  QVVNFVTGRWGIPTERIPAALNGVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYNHP 124

Query: 176 ILDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVI 235
           +  PFQR Y+ H    L+  AM+ AA + +G+HDFS F   A    V + V+ + R D+ 
Sbjct: 125 VRSPFQRLYSLHVPRALDLRAMRLAARHLMGRHDFSTF--QAAGRPVQSAVRTLTRADIE 184

Query: 236 EMGALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIP 295
               L++L     GFLY  VR M   LL++G E + P+ +P I+A+ DR   A+    +P
Sbjct: 185 AQAPLVRLVFRADGFLYNMVRIMAGTLLEVGLERLDPEALPDIIASGDR---ARAGPTVP 244

Query: 296 PQGLCLVRINY 305
           P GLCL  + Y
Sbjct: 245 PHGLCLEEVEY 247

BLAST of CmoCh17G003910 vs. Swiss-Prot
Match: TRUA_CLOTH (tRNA pseudouridine synthase A OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) GN=truA PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.2e-45
Identity = 102/249 (40.96%), Postives = 144/249 (57.83%), Query Frame = 1

Query: 56  RLVIAYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQV 115
           +L+I YDGT Y GWQ Q++  T+Q  +E A+ G T  E+ DL  +GASRTD GVHA GQV
Sbjct: 5   KLIIEYDGTNYHGWQKQKNAKTVQDTIERAIKGLTG-EKVDL--IGASRTDFGVHALGQV 64

Query: 116 AHFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAIL 175
           A+FIT       DR   ALN +LP DI ++E      DFHAR+    K Y Y IYN    
Sbjct: 65  ANFITNSGIPG-DRFSYALNRMLPDDIVIKESQEVDMDFHARYKAKGKRYRYLIYNSQFP 124

Query: 176 DPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEM 235
               R  TYH  +KL++ +M+ AA+YF+G HDFSAF ++  + +    V+ I    +   
Sbjct: 125 SALLRHRTYHVSHKLDFESMQRAASYFLGTHDFSAFRSSGSSAK--TSVRTITDVSLERE 184

Query: 236 GALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQ 295
             +++ E+ G GFLY  VR +V  L+++G   I  D +P+I+ +R RK   +     P +
Sbjct: 185 DKIVKFEIAGDGFLYNMVRIIVGTLMEVGIGKIRADEIPQIIESRKRKRAGR---TAPAE 244

Query: 296 GLCLVRINY 305
           GL LV + Y
Sbjct: 245 GLYLVEVYY 244

BLAST of CmoCh17G003910 vs. Swiss-Prot
Match: TRUA_DESRM (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) GN=truA PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 8.0e-44
Identity = 101/251 (40.24%), Postives = 146/251 (58.17%), Query Frame = 1

Query: 56  RLVIAYDGTRYAGWQYQRSP--PTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWG 115
           +L +AYDGT Y G+Q QR     TIQ  +E+AL+    + +  + ++GA RTD GVHA G
Sbjct: 5   KLTLAYDGTNYHGFQEQRGTGLATIQEALEKALS---TIAQTPIQVIGAGRTDAGVHAQG 64

Query: 116 QVAHFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDA 175
           QV +F +       ++   ALN LLP DI+V +      DFHARFS ++K Y Y IY+  
Sbjct: 65  QVVNFRSEKWPVPAEKAPLALNVLLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHR 124

Query: 176 ILDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVI 235
           ++ PF R Y YH   +L+  AM+E A Y +G +DF +F   A    V + ++ I+R D+I
Sbjct: 125 VMSPFHRYYCYHEPRRLDVNAMQEGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADII 184

Query: 236 EMGALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIP 295
           E   +I L + G+GFLY  VR +   LL IG   I P+ + KI+ +++R       T  P
Sbjct: 185 EDAPVINLYLRGNGFLYNMVRIITGTLLNIGFGKIKPEDMVKIIESKNRTLAG---TTAP 244

Query: 296 PQGLCLVRINY 305
           PQGLCL+ + Y
Sbjct: 245 PQGLCLMEVEY 247

BLAST of CmoCh17G003910 vs. TrEMBL
Match: A0A0A0KB67_CUCSA (tRNA pseudouridine synthase OS=Cucumis sativus GN=Csa_6G001730 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 1.4e-159
Identity = 270/322 (83.85%), Postives = 292/322 (90.68%), Query Frame = 1

Query: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISED-SSCVTEPDFPSFKWRLVI 60
           M  WAVGS VLP +   LSP IPS NG SS + RGFP +ED +S + EP FP FKWRL+I
Sbjct: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFI 120
           AYDGTRYAGWQYQRSPPTIQCL+EEAL GAT+LERKDLHLVGASRTD GVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQ 180
           TPFNYDSL+ +HAALNGLLP DIRVREMSPAVPDFHARFSTLSKVYHYKIY+  I+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180

Query: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALI 240
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVIEMGAL+
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCL 300
           QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP++VPKILA+RDR+ELAKY + +PPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300

Query: 301 VRINYKEDHLKLPSDCPGTSFG 322
           V INYKEDHLKLPSDCPGTSFG
Sbjct: 301 VSINYKEDHLKLPSDCPGTSFG 322

BLAST of CmoCh17G003910 vs. TrEMBL
Match: A0A067G1J9_CITSI (tRNA pseudouridine synthase OS=Citrus sinensis GN=CISIN_1g043662mg PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.6e-134
Identity = 241/338 (71.30%), Postives = 270/338 (79.88%), Query Frame = 1

Query: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISEDSSCVTEP----DFPSFKWR 60
           MSY  VG   LPS P   +  +PS N      G G    E ++   +P     FP FKWR
Sbjct: 1   MSYGVVG---LPS-PLYSNGLLPSTNKQ----GVGVVGLEGTNNTVKPPTDDSFPGFKWR 60

Query: 61  LVIAYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVA 120
           LVIAYDGTRYAGWQ+Q SPPTIQC+VE+AL   T+LER+DL LVGASRTDTGVHAWGQVA
Sbjct: 61  LVIAYDGTRYAGWQFQTSPPTIQCIVEKALIRVTKLEREDLRLVGASRTDTGVHAWGQVA 120

Query: 121 HFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILD 180
           HFITPF+YD L+ IHAALNGLLP DIRVRE+S AVP+FHARFS  SK+YHYKIYNDA++D
Sbjct: 121 HFITPFSYDGLESIHAALNGLLPPDIRVREISAAVPEFHARFSAKSKIYHYKIYNDAVMD 180

Query: 181 PFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMG 240
           PFQR Y YH VYKLN   M++AA YFVGKHDFSAF N   NDR++NPVK +FRFDVIEMG
Sbjct: 181 PFQRHYAYHSVYKLNTTLMRQAAEYFVGKHDFSAFVNVQRNDRLLNPVKSVFRFDVIEMG 240

Query: 241 ALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQG 300
           AL+QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILAT+DRKELAKY    PP G
Sbjct: 241 ALLQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATQDRKELAKYALSAPPHG 300

Query: 301 LCLVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335
           LCLV + Y E+HL+LP+ CP +SFGR HSV KCKLPFY
Sbjct: 301 LCLVEVKYNEEHLRLPAACPLSSFGRHHSVRKCKLPFY 330

BLAST of CmoCh17G003910 vs. TrEMBL
Match: V4RXK8_9ROSI (tRNA pseudouridine synthase OS=Citrus clementina GN=CICLE_v10005313mg PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 5.8e-134
Identity = 242/338 (71.60%), Postives = 268/338 (79.29%), Query Frame = 1

Query: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISEDSSCVTEP----DFPSFKWR 60
           MSY  VG   LPS P   +  +PS N      G G    E ++   +P     FP FKWR
Sbjct: 1   MSYGVVG---LPS-PLYSNGLLPSTNKQ----GVGVVGLEGTNNTVKPPTDDSFPGFKWR 60

Query: 61  LVIAYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVA 120
           LVIAYDGTRYAGWQ+Q SPPTIQC+VE+AL   T+LER+DL LVGASRTDTGVHAWGQVA
Sbjct: 61  LVIAYDGTRYAGWQFQTSPPTIQCIVEKALIRVTKLEREDLRLVGASRTDTGVHAWGQVA 120

Query: 121 HFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILD 180
           HFITPF+YD L+ IHAALNGLLP DIRVRE+S AVP+FHARFS  SK+YHYKIYNDA++D
Sbjct: 121 HFITPFSYDGLESIHAALNGLLPPDIRVREISAAVPEFHARFSAKSKIYHYKIYNDAVMD 180

Query: 181 PFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMG 240
           PFQ  Y YH VYKLN   M+EAA YFVGKHDFSAF N   NDR +NPVK IFRFDVIEMG
Sbjct: 181 PFQHHYAYHSVYKLNTTLMREAAEYFVGKHDFSAFVNIQRNDRALNPVKSIFRFDVIEMG 240

Query: 241 ALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQG 300
           AL+QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILAT+DRKELAKY    PP G
Sbjct: 241 ALLQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATQDRKELAKYALSAPPHG 300

Query: 301 LCLVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335
           LCLV + Y E+HL+LP+ CP +SFGR HSV KCKLPFY
Sbjct: 301 LCLVEVKYNEEHLRLPAACPLSSFGRHHSVRKCKLPFY 330

BLAST of CmoCh17G003910 vs. TrEMBL
Match: A0A067K865_JATCU (tRNA pseudouridine synthase OS=Jatropha curcas GN=JCGZ_19094 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 9.9e-134
Identity = 229/322 (71.12%), Postives = 267/322 (82.92%), Query Frame = 1

Query: 15  PRLLSPSIPSRNGDSSPTGRGFPISEDSSCVTEPDF--PSFKWRLVIAYDGTRYAGWQYQ 74
           P L  P +   + +++       +++D++  T+      +FKWRLVIAYDGTRYAGWQ+Q
Sbjct: 11  PSLHHPRLSVSSFNNNNNNNRLDVADDANYTTKQSTGADAFKWRLVIAYDGTRYAGWQFQ 70

Query: 75  RSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRIHA 134
           +SPPTIQC+VE+AL  AT+LERKDLHLVGASRTDTGVHAWGQVAHF+TPFNYDSLD IHA
Sbjct: 71  QSPPTIQCIVEKALVRATKLERKDLHLVGASRTDTGVHAWGQVAHFVTPFNYDSLDSIHA 130

Query: 135 ALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNY 194
           ALNGLLP DIRVRE+SPA+  FHARFS  SK+YHYKIYND+I+DPFQR Y  H  Y+LN 
Sbjct: 131 ALNGLLPPDIRVREISPALAQFHARFSARSKIYHYKIYNDSIMDPFQRQYACHTAYRLNA 190

Query: 195 AAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLYRQ 254
           AAM++AA YF+GKHDFSAFAN + NDRV +PVK I+RFDVIEMGAL+QLEVEG+GFLYRQ
Sbjct: 191 AAMRQAAQYFIGKHDFSAFANASRNDRVPDPVKTIYRFDVIEMGALLQLEVEGTGFLYRQ 250

Query: 255 VRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCLVRINYKEDHLKLP 314
           VRNMVALLLQIGKEAIPP+IVPKILATRDRKELAKYT   PP GLCLV +NY E+ L+LP
Sbjct: 251 VRNMVALLLQIGKEAIPPEIVPKILATRDRKELAKYTLSAPPHGLCLVAVNYTEEQLRLP 310

Query: 315 SDCPGTSFGRSHSVSKCKLPFY 335
           +DCP  SFGR  S+ KCKLP Y
Sbjct: 311 ADCPAISFGRHRSIGKCKLPLY 332

BLAST of CmoCh17G003910 vs. TrEMBL
Match: D7T696_VITVI (tRNA pseudouridine synthase OS=Vitis vinifera GN=VIT_05s0020g00860 PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 2.4e-132
Identity = 233/334 (69.76%), Postives = 265/334 (79.34%), Query Frame = 1

Query: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISEDSSCVTEPDFPSFKWRLVIA 60
           MSY  VG         L +P + S +     +G G     DS  +       +KWRLVIA
Sbjct: 1   MSYGVVGG--------LAAPPLNSLSDGGGGSGGGGGGGGDSIHLGNTSASGYKWRLVIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFIT 120
           Y+GTRY+GWQYQ+SPPTIQC+VE+ALT AT+LER+DL+LVGASRTDTGVHAWGQVAHF+T
Sbjct: 61  YEGTRYSGWQYQQSPPTIQCIVEKALTRATKLEREDLYLVGASRTDTGVHAWGQVAHFLT 120

Query: 121 PFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQR 180
           PFNYD L+ IHAALNGLLP DIRVRE+SPAVP+FHARFS  SK+YHYKIYNDA++DPF R
Sbjct: 121 PFNYDGLESIHAALNGLLPPDIRVREISPAVPEFHARFSVKSKIYHYKIYNDAVMDPFLR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQ 240
            Y YH VYKLN  AM+EAA +F GKHDFSAF N +HNDR  NPVK IFRFDV EMGAL+Q
Sbjct: 181 HYVYHSVYKLNTLAMREAAKHFTGKHDFSAFVNASHNDRSPNPVKNIFRFDVNEMGALLQ 240

Query: 241 LEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCLV 300
           LEVEGSGFLYRQVRNMVALLLQIG+EAIPP+IVP ILATRDRKELAKY    PP GLCL+
Sbjct: 241 LEVEGSGFLYRQVRNMVALLLQIGREAIPPNIVPWILATRDRKELAKYALSAPPHGLCLM 300

Query: 301 RINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335
            + Y E+HL+LPS CP TS GR H+V KCKLPFY
Sbjct: 301 SVKYNEEHLQLPSGCPATSHGRHHTVRKCKLPFY 326

BLAST of CmoCh17G003910 vs. TAIR10
Match: AT3G06950.1 (AT3G06950.1 Pseudouridine synthase family protein)

HSP 1 Score: 438.7 bits (1127), Expect = 3.2e-123
Identity = 216/328 (65.85%), Postives = 254/328 (77.44%), Query Frame = 1

Query: 8   SAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISEDSSCVTEPDFPSFKWRLVIAYDGTRYA 67
           S V P++P    PS+ +    SSP  +   ++  +S V   D  ++KWRLVIAYDGTR+A
Sbjct: 2   SCVTPALP----PSLFTNGYLSSP--QNISMTNLNSSVKVSDSGAYKWRLVIAYDGTRFA 61

Query: 68  GWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSL 127
           GWQYQ SPPTIQ ++E+AL   T L RK+L L+GA RTD GVHAWGQVAHF+TPFNY SL
Sbjct: 62  GWQYQESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTSL 121

Query: 128 DRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCV 187
           D  HAALNGLLP DIRVRE+S AVP+FHARFS  SKVY Y+IYND  +DPFQR + YHC 
Sbjct: 122 DSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHCA 181

Query: 188 YKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSG 247
           YKLN + M+EAAN FVGKHDFSAFAN    D V +P+K I RFDVI+MG+L+QLEVEGSG
Sbjct: 182 YKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGSG 241

Query: 248 FLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTC-IPPQGLCLVRINYKE 307
           FLYRQVRNMVALL+QIGKEA+  DIVP IL T+DR+ LAKYT+    P GLCLV + YKE
Sbjct: 242 FLYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYKE 301

Query: 308 DHLKLPSDCPGTSFGRSHSVSKCKLPFY 335
           DHLKLP DCP TS GR H+++KCKLPFY
Sbjct: 302 DHLKLPLDCPVTSSGRHHTITKCKLPFY 323

BLAST of CmoCh17G003910 vs. TAIR10
Match: AT1G34150.1 (AT1G34150.1 Pseudouridine synthase family protein)

HSP 1 Score: 85.1 bits (209), Expect = 8.9e-17
Identity = 80/258 (31.01%), Postives = 111/258 (43.02%), Query Frame = 1

Query: 57  LVIAYDGTRYAGWQYQ-RSPPTIQCLVEEALTGATRL---ERKDLHLVGASRTDTGVHAW 116
           L I Y G R+ G+  + +  P+I+  V +AL   TRL   ++KD       RTD GV + 
Sbjct: 94  LKIMYFGKRFYGFSAEAQMEPSIESEVFKALE-RTRLLVGDKKDSCYSRCGRTDKGVSST 153

Query: 117 GQVAHFITPFNY-----DSLDRIHA------------ALNGLLPADIRVREMSPAVPDFH 176
           GQV              DS  +++              LN  LP DIRV   SPA  DFH
Sbjct: 154 GQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFH 213

Query: 177 ARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAF---- 236
           ARFS  ++ Y Y  +                   LN +AM  A   F+G+HDF  F    
Sbjct: 214 ARFSCYAREYKYFFWRQ----------------NLNLSAMDFAGKKFIGEHDFRNFCKMD 273

Query: 237 -ANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPP 289
            AN     R V   +V    +  E   L    + GS FL+ Q+R MVA+L  IG+     
Sbjct: 274 VANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAFLWHQIRCMVAVLFMIGQGVESV 333

BLAST of CmoCh17G003910 vs. TAIR10
Match: AT1G09800.1 (AT1G09800.1 Pseudouridine synthase family protein)

HSP 1 Score: 66.2 bits (160), Expect = 4.3e-11
Identity = 52/202 (25.74%), Postives = 94/202 (46.53%), Query Frame = 1

Query: 54  KWRLVIAYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWG 113
           ++ + I Y GTR++G Q Q    T+  +++EA     +   + + +  +SRTD GVHA  
Sbjct: 56  RYLVAIEYIGTRFSGSQQQAKDRTVVGVLQEAFH---KFIGQPVKIFCSSRTDAGVHALS 115

Query: 114 QVAHF------------ITPFNYDSLDRIHAALNGLLP---ADIRVREMSPAVPDFHARF 173
            V H             + P +   +  +  A+N  L     D+ V ++     ++HAR+
Sbjct: 116 NVCHVDVERISKRKPGEVLPPHEPGV--VQRAVNHFLQRKDGDVMVIDVRCVPSNYHARY 175

Query: 174 STLSKVYHYKIYNDAILDPFQ-----RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFAN 233
               + Y Y++ + +  DP       RC+  H   +LN   M+EA    VG HDFS+F  
Sbjct: 176 KAQERTYFYRLLSGS--DPLSILEKDRCW--HVPEELNLRFMQEACRVLVGSHDFSSF-- 235

Query: 234 TAHNDRVVNPVKVIFRFDVIEM 236
            A   +  +P++ +   ++ E+
Sbjct: 236 RAAGCQAKSPMRSLDELNITEV 246

BLAST of CmoCh17G003910 vs. NCBI nr
Match: gi|659116826|ref|XP_008458279.1| (PREDICTED: tRNA pseudouridine synthase-like 1 isoform X1 [Cucumis melo])

HSP 1 Score: 598.2 bits (1541), Expect = 8.8e-168
Identity = 281/334 (84.13%), Postives = 302/334 (90.42%), Query Frame = 1

Query: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISEDSSCVTEPDFPSFKWRLVIA 60
           M  WAVGS VLP +    SP IPS NG SS + RGFP +EDSS +TEP FPSFKWRL+IA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCL+EEAL GAT+LERKDLHLVGASRTD GVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQR 180
           PFNYDSL+ +HAALNGLLP DIRVREMSPA PDFHARFSTLSKVYHYKIYN  I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQ 240
           CYTYHCVYKLNYAAMKEAANYFVGKHDFSAF N AHN+RVVNPVKVIFRFDVIEMGAL+Q
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCLV 300
           LEVEGSGFLYRQVRNMV LL+QIGKEA+PP++VPKILA+RDRKELAKY + +PPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 RINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335
            INYKEDHLKLPSDCPGTS GRS SV KCK+PFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

BLAST of CmoCh17G003910 vs. NCBI nr
Match: gi|778709015|ref|XP_004138518.2| (PREDICTED: tRNA pseudouridine synthase-like 1 [Cucumis sativus])

HSP 1 Score: 593.2 bits (1528), Expect = 2.8e-166
Identity = 280/335 (83.58%), Postives = 304/335 (90.75%), Query Frame = 1

Query: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISED-SSCVTEPDFPSFKWRLVI 60
           M  WAVGS VLP +   LSP IPS NG SS + RGFP +ED +S + EP FP FKWRL+I
Sbjct: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80

Query: 61  AYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFI 120
           AYDGTRYAGWQYQRSPPTIQCL+EEAL GAT+LERKDLHLVGASRTD GVHAWGQVAHF+
Sbjct: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140

Query: 121 TPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQ 180
           TPFNYDSL+ +HAALNGLLP DIRVREMSPAVPDFHARFSTLSKVYHYKIY+  I+DPFQ
Sbjct: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200

Query: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALI 240
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVIEMGAL+
Sbjct: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260

Query: 241 QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCL 300
           QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP++VPKILA+RDR+ELAKY + +PPQGLCL
Sbjct: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320

Query: 301 VRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335
           V INYKEDHLKLPSDCPGTSFGR+ SVSKCK+PFY
Sbjct: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 355

BLAST of CmoCh17G003910 vs. NCBI nr
Match: gi|700190423|gb|KGN45627.1| (hypothetical protein Csa_6G001730 [Cucumis sativus])

HSP 1 Score: 570.5 bits (1469), Expect = 2.0e-159
Identity = 270/322 (83.85%), Postives = 292/322 (90.68%), Query Frame = 1

Query: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISED-SSCVTEPDFPSFKWRLVI 60
           M  WAVGS VLP +   LSP IPS NG SS + RGFP +ED +S + EP FP FKWRL+I
Sbjct: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFI 120
           AYDGTRYAGWQYQRSPPTIQCL+EEAL GAT+LERKDLHLVGASRTD GVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQ 180
           TPFNYDSL+ +HAALNGLLP DIRVREMSPAVPDFHARFSTLSKVYHYKIY+  I+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180

Query: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALI 240
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVIEMGAL+
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCL 300
           QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP++VPKILA+RDR+ELAKY + +PPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300

Query: 301 VRINYKEDHLKLPSDCPGTSFG 322
           V INYKEDHLKLPSDCPGTSFG
Sbjct: 301 VSINYKEDHLKLPSDCPGTSFG 322

BLAST of CmoCh17G003910 vs. NCBI nr
Match: gi|659116828|ref|XP_008458280.1| (PREDICTED: tRNA pseudouridine synthase-like 1 isoform X2 [Cucumis melo])

HSP 1 Score: 496.1 bits (1276), Expect = 4.7e-137
Identity = 231/263 (87.83%), Postives = 247/263 (93.92%), Query Frame = 1

Query: 72  QRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRIH 131
           QRSPPTIQCL+EEAL GAT+LERKDLHLVGASRTD GVHAWGQVAHF+TPFNYDSL+ +H
Sbjct: 42  QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVTPFNYDSLESVH 101

Query: 132 AALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLN 191
           AALNGLLP DIRVREMSPA PDFHARFSTLSKVYHYKIYN  I+DPFQRCYTYHCVYKLN
Sbjct: 102 AALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQRCYTYHCVYKLN 161

Query: 192 YAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLYR 251
           YAAMKEAANYFVGKHDFSAF N AHN+RVVNPVKVIFRFDVIEMGAL+QLEVEGSGFLYR
Sbjct: 162 YAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQLEVEGSGFLYR 221

Query: 252 QVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCLVRINYKEDHLKL 311
           QVRNMV LL+QIGKEA+PP++VPKILA+RDRKELAKY + +PPQGLCLV INYKEDHLKL
Sbjct: 222 QVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLVSINYKEDHLKL 281

Query: 312 PSDCPGTSFGRSHSVSKCKLPFY 335
           PSDCPGTS GRS SV KCK+PFY
Sbjct: 282 PSDCPGTSLGRSRSVGKCKVPFY 304

BLAST of CmoCh17G003910 vs. NCBI nr
Match: gi|641853366|gb|KDO72225.1| (hypothetical protein CISIN_1g043662mg [Citrus sinensis])

HSP 1 Score: 486.5 bits (1251), Expect = 3.7e-134
Identity = 241/338 (71.30%), Postives = 270/338 (79.88%), Query Frame = 1

Query: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISEDSSCVTEP----DFPSFKWR 60
           MSY  VG   LPS P   +  +PS N      G G    E ++   +P     FP FKWR
Sbjct: 1   MSYGVVG---LPS-PLYSNGLLPSTNKQ----GVGVVGLEGTNNTVKPPTDDSFPGFKWR 60

Query: 61  LVIAYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVA 120
           LVIAYDGTRYAGWQ+Q SPPTIQC+VE+AL   T+LER+DL LVGASRTDTGVHAWGQVA
Sbjct: 61  LVIAYDGTRYAGWQFQTSPPTIQCIVEKALIRVTKLEREDLRLVGASRTDTGVHAWGQVA 120

Query: 121 HFITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILD 180
           HFITPF+YD L+ IHAALNGLLP DIRVRE+S AVP+FHARFS  SK+YHYKIYNDA++D
Sbjct: 121 HFITPFSYDGLESIHAALNGLLPPDIRVREISAAVPEFHARFSAKSKIYHYKIYNDAVMD 180

Query: 181 PFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMG 240
           PFQR Y YH VYKLN   M++AA YFVGKHDFSAF N   NDR++NPVK +FRFDVIEMG
Sbjct: 181 PFQRHYAYHSVYKLNTTLMRQAAEYFVGKHDFSAFVNVQRNDRLLNPVKSVFRFDVIEMG 240

Query: 241 ALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQG 300
           AL+QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILAT+DRKELAKY    PP G
Sbjct: 241 ALLQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATQDRKELAKYALSAPPHG 300

Query: 301 LCLVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335
           LCLV + Y E+HL+LP+ CP +SFGR HSV KCKLPFY
Sbjct: 301 LCLVEVKYNEEHLRLPAACPLSSFGRHHSVRKCKLPFY 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TRUA1_PARUW6.5e-5440.78tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) GN=... [more]
TRUA_CALS45.0e-4644.35tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongen... [more]
TRUA_DESAP6.5e-4641.83tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) GN=tru... [more]
TRUA_CLOTH7.2e-4540.96tRNA pseudouridine synthase A OS=Clostridium thermocellum (strain ATCC 27405 / D... [more]
TRUA_DESRM8.0e-4440.24tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) GN=truA... [more]
Match NameE-valueIdentityDescription
A0A0A0KB67_CUCSA1.4e-15983.85tRNA pseudouridine synthase OS=Cucumis sativus GN=Csa_6G001730 PE=3 SV=1[more]
A0A067G1J9_CITSI2.6e-13471.30tRNA pseudouridine synthase OS=Citrus sinensis GN=CISIN_1g043662mg PE=3 SV=1[more]
V4RXK8_9ROSI5.8e-13471.60tRNA pseudouridine synthase OS=Citrus clementina GN=CICLE_v10005313mg PE=3 SV=1[more]
A0A067K865_JATCU9.9e-13471.12tRNA pseudouridine synthase OS=Jatropha curcas GN=JCGZ_19094 PE=3 SV=1[more]
D7T696_VITVI2.4e-13269.76tRNA pseudouridine synthase OS=Vitis vinifera GN=VIT_05s0020g00860 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G06950.13.2e-12365.85 Pseudouridine synthase family protein[more]
AT1G34150.18.9e-1731.01 Pseudouridine synthase family protein[more]
AT1G09800.14.3e-1125.74 Pseudouridine synthase family protein[more]
Match NameE-valueIdentityDescription
gi|659116826|ref|XP_008458279.1|8.8e-16884.13PREDICTED: tRNA pseudouridine synthase-like 1 isoform X1 [Cucumis melo][more]
gi|778709015|ref|XP_004138518.2|2.8e-16683.58PREDICTED: tRNA pseudouridine synthase-like 1 [Cucumis sativus][more]
gi|700190423|gb|KGN45627.1|2.0e-15983.85hypothetical protein Csa_6G001730 [Cucumis sativus][more]
gi|659116828|ref|XP_008458280.1|4.7e-13787.83PREDICTED: tRNA pseudouridine synthase-like 1 isoform X2 [Cucumis melo][more]
gi|641853366|gb|KDO72225.1|3.7e-13471.30hypothetical protein CISIN_1g043662mg [Citrus sinensis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001406PsdUridine_synth_TruA
IPR020094Pseudouridine synthase I, TruA, N-terminal
IPR020095PsdUridine_synth_TruA_C
IPR020097PsdUridine_synth_TruA_a/b_dom
IPR020103PsdUridine_synth_cat_dom_sf
Vocabulary: Biological Process
TermDefinition
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
Vocabulary: Molecular Function
TermDefinition
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006730 one-carbon metabolic process
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0008033 tRNA processing
biological_process GO:0009451 RNA modification
cellular_component GO:0005575 cellular_component
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh17G003910.1CmoCh17G003910.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001406Pseudouridine synthase I, TruAHAMAPMF_00171TruAcoord: 53..304
score: 32
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 54..322
score: 6.0E
IPR001406Pseudouridine synthase I, TruATIGRFAMsTIGR00071TIGR00071coord: 57..288
score: 1.3
IPR020094Pseudouridine synthase I, TruA, N-terminalGENE3DG3DSA:3.30.70.580coord: 54..159
score: 2.7
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3DG3DSA:3.30.70.660coord: 162..302
score: 5.8
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 198..304
score: 2.6E-25coord: 59..158
score: 2.
IPR020103Pseudouridine synthase, catalytic domainunknownSSF55120Pseudouridine synthasecoord: 53..310
score: 3.31
NoneNo IPR availablePANTHERPTHR11142:SF0TRNA PSEUDOURIDINE SYNTHASE-LIKE 1coord: 54..322
score: 6.0E